Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,577,657 A→G 5.5% intergenic (‑65/+74) gntK ← / ← gntR gluconate kinase 2/d‑gluconate inducible gluconate regulon transcriptional repressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,577,6570AG 5.5% 650.3 / 16.0 195intergenic (‑65/+74)gntK/gntRgluconate kinase 2/d‑gluconate inducible gluconate regulon transcriptional repressor
Reads supporting (aligned to +/- strand):  ref base A (86/98);  new base G (4/7);  total (90/105)
Fisher's exact test for biased strand distribution p-value = 5.51e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

Only 100 of 199 total aligned reads displayed.

CACTTCACTGGCGACCGCAGATTTGCCGCTGCCCGATACGCCCATCAAGACGTAAATGTGGTGATCATGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAATAGATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACG  >  NC_000913/3577509‑3577805
                                                                                                                                                    |                                                                                                                                                    
cACTTCACTGGCGACCGCAGATTTGCCGCTGCCCGATACGCCCATCAAGACGTAAATGTGGTGATCATGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCa                                                                                                                                                      >  1:828028/1‑149 (MQ=255)
    tCACTGGCGACCGCAGATTTGCCGCTGCCCGATACGCCCATCAAGACGTAAATGTGGTGATCATGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCg                                                                                                                                                  <  1:191967/149‑1 (MQ=255)
     cACTGGCGACCGCAGATTTGCCGCTGCCCGATACGCCCATCAAGACGTAAATGTGGTGATCATGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCgg                                                                                                                                                 <  2:605187/149‑1 (MQ=255)
       cTGGCGACCGCAGATTTGCCGCTGCCCGATACGCCCATCAAGACGTAAATGTGGTGATCATGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGAc                                                                                                                                               <  2:1399039/149‑1 (MQ=255)
           cGACCGCAGATTTGCCGCTGCCCGATACGCCCATCAAGACGTAAATGTGGTGATCATGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCGGCTGGACAatg                                                                                                                                           <  3:1429024/149‑2 (MQ=255)
           cGACCGCAGATTTGCCGCTGCCCGATACGCCCATCAAGACGTAAATGTGGTGATCATGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCGGCTGGACAatg                                                                                                                                           <  4:1593386/149‑2 (MQ=255)
              ccGCAGATTTGCCGCTGCCCGATACGCCCATCAAGACGTAAATGTGGTGATCATGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCa                                                                                                                                        <  4:1191617/149‑1 (MQ=255)
               cGCAGATTTGCCGCTGCCCGATACGCCCATCAAGACGTAAATGTGGTGATCATGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCGGCTGGACAatgttac                                                                                                                                       <  1:197445/149‑6 (MQ=255)
               cGCAGATTTGCCGCTGCCCGATACGCCCATCAAGACGTAAATGTGGTGATCATGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAg                                                                                                                                       >  3:510336/1‑149 (MQ=255)
                 cAGATTTGCCGCTGCCCGATACGCCCATCAAGACGTAAATGTGGTGATCATGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGaa                                                                                                                                     >  3:1140805/1‑149 (MQ=255)
                   gATTTGCCGCTGCCCGATACGCCAATCAAGACGTAAATGTGGTGATCATGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAACATCAGAAGc                                                                                                                                   <  4:1449696/149‑1 (MQ=255)
                                   atccGCCCATCAAGACGTAAATGTGGTGATCATGGTTAGTCGTGCTCAAAGTGGTGCCCCTACAATACACGACATAAAAATGTTACGGGTAACTGTTATCGGTATCATTGTCCAGCCGGACAATATCAGCAGCAATATCCATCCATGcc                                                                                                                   <  4:282379/146‑1 (MQ=255)
                                       gCCCATCAAGACGTAAATGTGGTGATCATGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTaa                                                                                                               <  3:642621/149‑1 (MQ=255)
                                       gCCCATCAAGACGTAAATGTGGTGATCATGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTaa                                                                                                               >  2:86609/1‑149 (MQ=255)
                                         ccATCAAGACGTAAATGTGGTGGTCATGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACAGTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAgt                                                                                                             >  4:1051390/1‑149 (MQ=255)
                                         ccATCAAGACGTAAATGTGGTGATCATGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGaa                                                                                                                                     <  2:435752/125‑1 (MQ=255)
                                         ccATCAAGACGTAAATGTGGTGATCATGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGaa                                                                                                                                     >  1:435752/1‑125 (MQ=255)
                                         ccATCAAGACGTAAATGTGGTGATCATGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAgt                                                                                                             <  3:1283888/149‑1 (MQ=255)
                                         ccATCAAGACGTAAATGTGGTGATCATGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAgt                                                                                                             >  1:1066766/1‑149 (MQ=255)
                                         ccATCAAGACGTAAATGTGGTGATCATGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAgt                                                                                                             <  1:665804/149‑1 (MQ=255)
                                             cAAGACGTAAATGTGGTGATCATGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGtat                                                                                                         <  3:1408287/149‑1 (MQ=255)
                                                 aCGTAAATGTGGTGATCATGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAgt                                                                                                     <  3:1620463/149‑1 (MQ=255)
                                                     aaaTGTGGTGATCATGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATGCAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGa                                                                                                 <  2:1602566/149‑1 (MQ=255)
                                                        tgtgGTGATCATGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCt                                                                                              >  1:917184/1‑149 (MQ=255)
                                                          ttgtgATCATGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGACTTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCATCCGGACAATATCTGAAGCAATATCCATCCATTCCTTAAGTGTATAAGTGTCAGCTAc                                                                                            <  4:1075554/147‑1 (MQ=255)
                                                          tggtgATCATGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTAc                                                                                            >  1:1084586/1‑149 (MQ=255)
                                                               aTCATGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCaa                                                                                       <  4:908793/149‑1 (MQ=255)
                                                                tCATGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTaa                                                                                                               >  1:783034/1‑124 (MQ=255)
                                                                  aTGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAAtt                                                                                    >  1:628732/1‑149 (MQ=255)
                                                                     gTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTtgt                                                                                 <  1:535956/149‑1 (MQ=255)
                                                                     gTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTtgt                                                                                 <  3:1649181/149‑1 (MQ=255)
                                                                     gTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTtgt                                                                                 <  2:1518215/149‑1 (MQ=255)
                                                                      ttAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTtgtg                                                                                <  2:1055455/149‑1 (MQ=255)
                                                                      ttAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTtgtg                                                                                <  1:457077/149‑1 (MQ=255)
                                                                       tAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGATAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTgg                                                                               <  2:1084586/149‑1 (MQ=255)
                                                                       tAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTgg                                                                               <  3:111783/149‑1 (MQ=255)
                                                                        aGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTggg                                                                              <  4:780643/149‑1 (MQ=255)
                                                                         gTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGc                                                                             <  2:380003/149‑1 (MQ=255)
                                                                          tCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCt                                                                            >  1:584587/1‑149 (MQ=255)
                                                                              gCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTaaa                                                                        <  1:1496419/149‑1 (MQ=255)
                                                                               cTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATAtccatcca                                                                                                                       <  3:1583358/101‑1 (MQ=255)
                                                                               cTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATAtccatcca                                                                                                                       >  4:1583358/1‑101 (MQ=255)
                                                                               cTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAAt                                                                       <  2:688221/149‑1 (MQ=255)
                                                                                tCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTaaa                                                                        >  2:568496/1‑147 (MQ=255)
                                                                                tCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAATa                                                                      >  2:1103716/1‑149 (MQ=255)
                                                                                tCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAATa                                                                      >  1:275490/1‑149 (MQ=255)
                                                                                tCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAATa                                                                      >  3:533913/1‑149 (MQ=255)
                                                                                tCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAATa                                                                      >  1:56598/1‑149 (MQ=255)
                                                                                tCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAATa                                                                      >  4:123310/1‑149 (MQ=255)
                                                                                tCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAATa                                                                      >  2:1578088/1‑149 (MQ=255)
                                                                                  aaaGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAATAGa                                                                    <  1:1126344/149‑1 (MQ=255)
                                                                                   aaGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAATAGAt                                                                   <  1:1456730/149‑1 (MQ=255)
                                                                                             cccACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAATAGATCCGCCCGGTg                                                         >  1:1663679/1‑149 (MQ=255)
                                                                                               cacaATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACa                                                                                                                                              <  2:295592/62‑1 (MQ=255)
                                                                                                      cAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAATAGATCCGCCCGGTGACAAGGTGa                                                >  1:815614/1‑149 (MQ=255)
                                                                                                      cAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAATAGATCCGCCCGGTGACAAGGTGa                                                >  4:659075/1‑149 (MQ=255)
                                                                                                       aaGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAATAGATCCGCCCGGTGACAAGGTGaa                                               >  1:1263599/1‑149 (MQ=255)
                                                                                                       aaGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAATAGATCCGCCCGGTGACAAGGTGaa                                               >  3:249368/1‑149 (MQ=255)
                                                                                                       aaGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAATAGATCCGCCCGGTGACAAGGTGaa                                               >  4:376475/1‑149 (MQ=255)
                                                                                                       aaGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAATAGATCCGCCCGGTGACAAGGTGaa                                               >  4:582866/1‑149 (MQ=255)
                                                                                                         gAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAg                                                                                                              <  2:1572619/84‑1 (MQ=255)
                                                                                                         gAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAg                                                                                                              >  1:1572619/1‑84 (MQ=255)
                                                                                                         gAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAATAGATCCGCCCGGTGACAAGGTGAAAc                                             <  3:595933/149‑1 (MQ=255)
                                                                                                         gAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAATAGATCCGCCCGGTGACAAGGTGAAAc                                             <  1:1578088/149‑1 (MQ=255)
                                                                                                          aaTTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAATAGATcc                                                                 <  4:544251/128‑1 (MQ=255)
                                                                                                              aaaaaTGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAATAGATCCGCCCGGTGACAAGGTGAAACCTaaa                                        <  3:123310/149‑1 (MQ=255)
                                                                                                               aaaaTGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAATAGATCCGCCCGGTGACAAGGTGAAACCTAAAt                                       >  2:915663/1‑149 (MQ=255)
                                                                                                               aaaaTGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAATAGATCCGCCCGGTGACAAGGTGAAACCTAAAt                                       <  2:195026/149‑1 (MQ=255)
                                                                                                               aaaaTGTTACGGGTAACTGGTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAATAGATCCGCCCGGTGACAAGGTGAAACCTAAAt                                       >  1:411788/1‑149 (MQ=255)
                                                                                                                aaaTGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAATAGATCCGCCCGGTGACAAGGTGAAACCTAAATc                                      >  4:1484383/1‑149 (MQ=255)
                                                                                                                aaaTGTTACGGGTAACTGCTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTATGAGCTACTTCAAATTTGTGGGCTTAAATAGATCCGCCCGGTGACAAGGt                                                  >  3:978406/1‑137 (MQ=255)
                                                                                                                 aaTGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTtgt                                                                                 <  3:1466952/105‑1 (MQ=255)
                                                                                                                 aaTGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTtgt                                                                                 >  4:1466952/1‑105 (MQ=255)
                                                                                                                   tGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAATAGATCCGCCCGGTGACAAGGTGAAACCTAAATCTaa                                   <  1:1054865/149‑1 (MQ=255)
                                                                                                                   tGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAATAGATCCGCCCGGTGACAAGGTGAAACCTAAATCTaa                                   <  3:376475/149‑1 (MQ=255)
                                                                                                                    gTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAATAGATCCGCCCGGTGACAAGGTGAAACCTAAATCTAAc                                  >  4:611191/1‑149 (MQ=255)
                                                                                                                     ttACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAATAGATCCGCCCGGt                                                          <  4:1121275/124‑1 (MQ=255)
                                                                                                                       aCGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAATAGATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACAtt                               <  3:215963/149‑1 (MQ=255)
                                                                                                                       aCGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAATAGATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACAtt                               <  2:1007916/149‑1 (MQ=255)
                                                                                                                         gggTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTgg                                                                               >  2:807086/1‑99 (MQ=255)
                                                                                                                           gTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAATAGATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCg                           <  2:116469/149‑1 (MQ=255)
                                                                                                                            tAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAATAGATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCgg                          >  1:345164/1‑149 (MQ=255)
                                                                                                                              aCTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTgg                                                                               >  2:1673008/1‑94 (MQ=255)
                                                                                                                                tGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAATAGATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTc                      >  3:1331231/1‑149 (MQ=255)
                                                                                                                                tGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAATAGATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTc                      >  1:1046552/1‑149 (MQ=255)
                                                                                                                                tGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCAATCCATGCTTTAAGTGTATAAgt                                                                                                     <  3:118407/70‑1 (MQ=255)
                                                                                                                                tGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCAATCCATGCCTTAAGTGTATACgt                                                                                                     >  4:118407/1‑70 (MQ=255)
                                                                                                                                 gTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAATAGATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTca                     <  4:360204/149‑1 (MQ=255)
                                                                                                                                  ttATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGta                                                                                                          >  3:1188938/1‑63 (MQ=255)
                                                                                                                                     tCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAATAGATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGa                 <  2:990410/149‑1 (MQ=255)
                                                                                                                                     tCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAATAGATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGa                 >  2:896222/1‑149 (MQ=255)
                                                                                                                                        gTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTgg                                                                               >  1:1466542/1‑84 (MQ=255)
                                                                                                                                        gTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTgg                                                                               <  2:1466542/84‑1 (MQ=255)
                                                                                                                                            taTTGTCCGGCTGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAATAGAt                                                                   <  3:1457665/91‑1 (MQ=255)
                                                                                                                                            cATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAATAGATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGgt                         >  1:1625093/1‑134 (MQ=255)
                                                                                                                                                gTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAATAGATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGaa      <  1:887485/149‑1 (MQ=255)
                                                                                                                                                 tCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAATAGATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACGGATTCTCCACGAAt     >  3:1044904/1‑149 (MQ=255)
                                                                                                                                                    aGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAATAGATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTc              >  1:1416342/1‑137 (MQ=255)
                                                                                                                                                    aGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAATAGATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTc              <  2:1416342/137‑1 (MQ=255)
                                                                                                                                                    aGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAATAGATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATAcg  >  3:75077/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CACTTCACTGGCGACCGCAGATTTGCCGCTGCCCGATACGCCCATCAAGACGTAAATGTGGTGATCATGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAATAGATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACG  >  NC_000913/3577509‑3577805

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: