Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,577,660 C→T 5.5% intergenic (‑68/+71) gntK ← / ← gntR gluconate kinase 2/d‑gluconate inducible gluconate regulon transcriptional repressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,577,6600CT 5.5% 659.6 / 18.3 198intergenic (‑68/+71)gntK/gntRgluconate kinase 2/d‑gluconate inducible gluconate regulon transcriptional repressor
Reads supporting (aligned to +/- strand):  ref base C (88/99);  new base T (4/7);  total (92/106)
Fisher's exact test for biased strand distribution p-value = 5.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.42e-01

Only 100 of 199 total aligned reads displayed.

TTCACTGGCGACCGCAGATTTGCCGCTGCCCGATACGCCCATCAAGACGTAAATGTGGTGATCATGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAATAGATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGC  >  NC_000913/3577512‑3577806
                                                                                                                                                    |                                                                                                                                                  
 tCACTGGCGACCGCAGATTTGCCGCTGCCCGATACGCCCATCAAGACGTAAATGTGGTGATCATGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCg                                                                                                                                                   <  1:191967/149‑1 (MQ=255)
            cGCAGATTTGCCGCTGCCCGATACGCCCATCAAGACGTAAATGTGGTGATCATGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCGGCTGGACAatgttac                                                                                                                                        <  1:197445/149‑6 (MQ=255)
            cGCAGATTTGCCGCTGCCCGATACGCCCATCAAGACGTAAATGTGGTGATCATGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCGGCTGGACAatgttac                                                                                                                                        <  4:1586181/149‑6 (MQ=255)
              cAGATTTGCCGCTGCCCGATACGCCCATCAAGACGTAAATGTGGTGATCATGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGaa                                                                                                                                      <  3:1296625/149‑1 (MQ=255)
              cAGATTTGCCGCTGCCCGATACGCCCATCAAGACGTAAATGTGGTGATCATGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGaa                                                                                                                                      <  3:1487330/149‑1 (MQ=255)
               aGATTTGCCGCTGCCCGATACGCCCATCAAGACGTAAATGTGGTGATCATGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAg                                                                                                                                     <  4:1425508/149‑1 (MQ=255)
                        gCTGCCCGATACGCCCATCAAGACGTAAATGTGGTGATCATGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCGGCTGGACAatg                                                                                                                                            <  2:3532/133‑2 (MQ=255)
                          tGCCCGATACGCCCATCAAGACGTAAATGTGGTGATCATGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATatccatc                                                                                                                          <  4:1140805/149‑1 (MQ=255)
                             ccGATACGCCCATCAAGACGTAAATGTGGTGATCATGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATccatccat                                                                                                                       <  3:1185293/149‑1 (MQ=255)
                                    gCCCATCAAGACGTAAATGTGGTGATCATGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAatat                                                                                                                               >  2:1576318/1‑134 (MQ=255)
                                      ccATCAAGACGTAAATGTGGTGATCATGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGaa                                                                                                                                      >  1:435752/1‑125 (MQ=255)
                                      ccATCAAGACGTAAATGTGGTGATCATGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAgt                                                                                                              <  3:748587/149‑1 (MQ=255)
                                      ccATCAAGACGTAAATGTGGTGATCATGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAgt                                                                                                              >  4:360685/1‑149 (MQ=255)
                                      ccATCAAGACGTAAATGTGGTGATCATGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAgt                                                                                                              <  1:665804/149‑1 (MQ=255)
                                      ccATCAAGACGTAAATGTGGTGATCATGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAgt                                                                                                              >  1:1066766/1‑149 (MQ=255)
                                        aTCAAGACGTAAATGTGGTGATCATGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAgtgt                                                                                                            <  4:1474551/149‑1 (MQ=255)
                                         tCAAGACGTAAATGTGGTGATCATGGTTAGTCGTGCTCAAATTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGta                                                                                                           >  1:934040/1‑149 (MQ=255)
                                         tCAAGACGTAAATGTGGTGATCATGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGta                                                                                                           >  3:1272804/1‑149 (MQ=255)
                                              aCGTAAATGTGGTGATCATGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAgt                                                                                                      <  3:982063/149‑1 (MQ=255)
                                              aCGTAAATATGGTGCTCATGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGCTAACTGTTATCGGTAACCTTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAgt                                                                                                      <  3:1413668/149‑1 (MQ=255)
                                                  aaaTGTGGTGATCATGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGa                                                                                                  <  3:1634849/149‑1 (MQ=255)
                                                  aaaTGTGGTGATCATGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGa                                                                                                  <  4:1066746/149‑1 (MQ=255)
                                                       tggtgATCATGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTAc                                                                                             <  3:267606/149‑1 (MQ=255)
                                                       tggtgATCATGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTAc                                                                                             >  1:1084586/1‑149 (MQ=255)
                                                           gATCATGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCTAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGa                                                                                                  >  2:1465344/1‑140 (MQ=255)
                                                            aTCATGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCaa                                                                                        <  4:908793/149‑1 (MQ=255)
                                                             tCATGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTaa                                                                                                                <  2:783034/124‑1 (MQ=255)
                                                             tCATGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTaa                                                                                                                >  1:783034/1‑124 (MQ=255)
                                                               aTGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTaa                                                                                                                >  1:908697/1‑122 (MQ=255)
                                                               aTGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTaa                                                                                                                <  2:908697/122‑1 (MQ=255)
                                                               aTGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAAtt                                                                                     >  1:628732/1‑149 (MQ=255)
                                                                tGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAAttt                                                                                    <  3:636906/149‑1 (MQ=255)
                                                                  gTTAGTCGTGCTCAAAGTGTTGCCCCAACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTtgt                                                                                  <  2:108876/149‑1 (MQ=255)
                                                                  gTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTtgt                                                                                  <  1:535956/149‑1 (MQ=255)
                                                                   ttAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTtgtg                                                                                 <  1:457077/149‑1 (MQ=255)
                                                                    tAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTgg                                                                                <  3:385075/149‑1 (MQ=255)
                                                                     aGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTggg                                                                               <  4:780643/149‑1 (MQ=255)
                                                                      gTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGc                                                                              <  4:1555779/149‑1 (MQ=255)
                                                                       tCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCt                                                                             <  4:322116/149‑1 (MQ=255)
                                                                       tCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCt                                                                             >  1:584587/1‑149 (MQ=255)
                                                                           gCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTaaa                                                                         <  1:1496419/149‑1 (MQ=255)
                                                                            cTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAAt                                                                        >  2:887485/1‑149 (MQ=255)
                                                                             tCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAATa                                                                       >  1:56598/1‑149 (MQ=255)
                                                                             tCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAATa                                                                       >  1:275490/1‑149 (MQ=255)
                                                                              cAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAATAg                                                                      <  2:275490/149‑1 (MQ=255)
                                                                               aaaGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGACGCAATATCCATCCATGCCTTAAGTGTATAAATGTGAGCTACTTCAAATTTGTGGTCTTAAATAGa                                                                     >  3:1011871/1‑149 (MQ=255)
                                                                               aaaGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAATAGa                                                                     <  1:1126344/149‑1 (MQ=255)
                                                                                aaGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAATAGAt                                                                    <  1:1456730/149‑1 (MQ=255)
                                                                                aaGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAATAGAt                                                                    <  2:934040/149‑1 (MQ=255)
                                                                                   tggtgCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAATAGATCCg                                                                 <  2:828028/149‑1 (MQ=255)
                                                                                       gCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAat                                                                                                                                 <  2:1414943/81‑1 (MQ=255)
                                                                                       gCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAGTATCTATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAATAGATCCGCCCg                                                             >  3:1223811/1‑149 (MQ=255)
                                                                                         ccccACAATACAAGAATTAAAAATGTTAAGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAATAGATCCGCCCGGt                                                           >  3:1079411/1‑149 (MQ=255)
                                                                                          cccACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAATAGATCCGCCCGGTg                                                          >  1:1663679/1‑149 (MQ=255)
                                                                                          cccACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAATAGATCCGCCCGGTg                                                          >  3:531438/1‑149 (MQ=255)
                                                                                          cccACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAATAGATCCGCCCGGTg                                                          >  2:1054865/1‑149 (MQ=255)
                                                                                           ccACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATaa                                                                                                        <  2:1241297/102‑1 (MQ=255)
                                                                                            cacaATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAATAGATCCGCCCGGTGAc                                                        <  3:144266/149‑1 (MQ=255)
                                                                                                 tACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAATAGATCCGCCCGGTGACAAGGt                                                   <  2:558993/149‑1 (MQ=255)
                                                                                                 tACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAATAGATCCGCCCGGTGACAAGGt                                                   <  4:1223811/149‑1 (MQ=255)
                                                                                                   cAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAATAGATCCGCCCGGTGACAAGGTGa                                                 >  4:659075/1‑149 (MQ=255)
                                                                                                   cAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAATAGATCCGCCCGGTGACAAGGTGa                                                 >  1:815614/1‑149 (MQ=255)
                                                                                                    aaGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAATAGATCCGCCCGGTGACAAGGTGaa                                                >  4:376475/1‑149 (MQ=255)
                                                                                                    aaGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAATAGATCCGCCCGGTGACAAGGTGaa                                                >  2:1542887/1‑149 (MQ=255)
                                                                                                    aaGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAATAGATCCGCCCGGTGACAAGGTGaa                                                >  4:582866/1‑149 (MQ=255)
                                                                                                    aaGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAATAGATCCGCCCGGTGACAAGGTGaa                                                >  1:1263599/1‑149 (MQ=255)
                                                                                                    aaGAATTAAAAATGTTACGGGTAACTGGTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAATAGATCCGCCCGGTGACAACGTGaa                                                >  3:1183254/1‑149 (MQ=255)
                                                                                                      gAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAg                                                                                                               >  1:1572619/1‑84 (MQ=255)
                                                                                                      gAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAATAGATCCGCCCGGTGACAAGGTGAAAc                                              <  1:1578088/149‑1 (MQ=255)
                                                                                                       aaTTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAATAGATcc                                                                  <  4:544251/128‑1 (MQ=255)
                                                                                                       aaTTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAATAGATcc                                                                  >  3:544251/1‑128 (MQ=255)
                                                                                                       aaTTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAATAGATCCGCCCGGTGACAAGGTGAAAcc                                             >  3:1372465/1‑149 (MQ=255)
                                                                                                            aaaaTGTTACGGGTAACTGGTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAATAGATCCGCCCGGTGACAAGGTGAAACCTAAAt                                        >  1:411788/1‑149 (MQ=255)
                                                                                                                tcttACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAATAGATCCGCCCGGTGACAAGGTGAAACCTAAATCTaa                                    <  4:1079411/147‑1 (MQ=255)
                                                                                                                tGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAATAGATCCGCCCGGTGACAAGGTGAAACCTAAATCTaa                                    <  1:1054865/149‑1 (MQ=255)
                                                                                                                 gTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAATAGATCCGCCCGGTGACAAGGTGAAACCTAAATCTAAc                                   >  4:611191/1‑149 (MQ=255)
                                                                                                                  tttcGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAATAGATCCGCCCGGt                                                           >  3:1121275/4‑124 (MQ=255)
                                                                                                                       ggTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAATAGATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTc                             <  2:1066766/149‑1 (MQ=255)
                                                                                                                       ggTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAATAGATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTc                             <  2:1590935/149‑1 (MQ=255)
                                                                                                                        gTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAATAGATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCg                            <  1:86609/149‑1 (MQ=255)
                                                                                                                        gTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAATAGATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCg                            >  2:1709371/1‑149 (MQ=255)
                                                                                                                        gTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAATAGATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCg                            >  2:41929/1‑149 (MQ=255)
                                                                                                                         tAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAATAGATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCgg                           >  1:345164/1‑149 (MQ=255)
                                                                                                                          aaCTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAATAGATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGgt                          <  2:1485963/149‑1 (MQ=255)
                                                                                                                          aaCTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAATAGATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGgt                          <  2:584587/149‑1 (MQ=255)
                                                                                                                             tGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAATAGATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTc                       >  1:1046552/1‑149 (MQ=255)
                                                                                                                               ttATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGGCTTAAGTGta                                                                                                           <  4:1188938/63‑1 (MQ=255)
                                                                                                                                  tCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAATAGATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGa                  >  3:771651/1‑149 (MQ=255)
                                                                                                                                  tCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAATAGATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGa                  <  3:1619238/149‑1 (MQ=255)
                                                                                                                                     gTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGta                                                                                                           <  3:219002/57‑1 (MQ=255)
                                                                                                                                     gTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGta                                                                                                           >  4:219002/1‑57 (MQ=255)
                                                                                                                                     gTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTgg                                                                                >  1:1466542/1‑84 (MQ=255)
                                                                                                                                       gataTTGTCCGGCTGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAAt                                                                        >  2:616180/4‑90 (MQ=255)
                                                                                                                                         taTTGTCCGGCTGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAATAGAt                                                                    >  4:1457665/2‑92 (MQ=255)
                                                                                                                                         cATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAATAGATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGgt                          <  2:1625093/134‑1 (MQ=255)
                                                                                                                                         cATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAATAGATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGgt                          >  1:1625093/1‑134 (MQ=255)
                                                                                                                                         cATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAATAGATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCa           >  3:1665364/1‑149 (MQ=255)
                                                                                                                                          aTTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAATAGATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCAc          >  3:1464282/1‑149 (MQ=255)
                                                                                                                                          aTTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAATAGATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCAc          >  2:1137413/1‑149 (MQ=255)
                                                                                                                                                 aGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAATAGATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTc               >  1:1416342/1‑137 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
TTCACTGGCGACCGCAGATTTGCCGCTGCCCGATACGCCCATCAAGACGTAAATGTGGTGATCATGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAATAGATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGC  >  NC_000913/3577512‑3577806

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: