Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,674,402 T→G 5.1% intergenic (+27/‑384) yhjD → / → yhjE inner membrane putative BrbK family alternate lipid exporter/putative MFS transporter; membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,674,4020TG 5.1% 487.2 / 16.1 138intergenic (+27/‑384)yhjD/yhjEinner membrane putative BrbK family alternate lipid exporter/putative MFS transporter; membrane protein
Reads supporting (aligned to +/- strand):  ref base T (58/73);  new base G (3/4);  total (61/77)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.45e-01

Only 100 of 140 total aligned reads displayed.

GCTGGGGCTGATGGCATTTTTCTACTTCTTCGCCCGTTTGACGCTGTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACCTCTTGAAACTCGTCCCCAGAGCCTCTTCAGCCATCTATTTGGGAGCAAACAATTTCATT  >  NC_000913/3674258‑3674550
                                                                                                                                                |                                                                                                                                                    
gCTGGGGCTGATGGCATTTTTCTACTTCTTCGCCCGTTTGACGCTGTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATc                                                                                                                                                  >  1:1686233/1‑149 (MQ=255)
 cTGGGGCTGATGGCATTTTTCTACTTCTTCGCCCGTTTGACGCTGTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATcc                                                                                                                                                 <  2:1360151/149‑1 (MQ=255)
   ggggCTGATGGCATTTTTCTACTTCTTCGCCCGTTTGACGCTGTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCgg                                                                                                                                               >  4:524209/1‑149 (MQ=255)
    gggCTGATGGCATTTTTCTACTTCTTCGCCCGTTTGACGCTGTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGc                                                                                                                                              <  2:621810/149‑1 (MQ=255)
            ggCATTTTTCTACTTCTTCGCCCGTTTGACGCTGTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTGTCCGGCATACGAtt                                                                                                                                      <  4:552319/149‑1 (MQ=255)
            ggCATTTTTCTACTTCTTCGCCCGTTTGACGCTGTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGAtt                                                                                                                                      <  3:555580/149‑1 (MQ=255)
            ggCATTTTTCTACTTCTTCGCCCGTTTGACGCTGTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGAtt                                                                                                                                      >  1:1404176/1‑149 (MQ=255)
             gCATTTTTCTACTTCTTCGCCCGTTTGACGCTGTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGAttt                                                                                                                                     <  2:1099149/149‑1 (MQ=255)
               aTTTTTCTACTTCTTCGCCCGTTTGACGCTGTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTa                                                                                                                                   >  2:118093/1‑149 (MQ=255)
                tttttCTACTTCTTCGCCCGTTTGACGCTGTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTAt                                                                                                                                  >  4:122144/1‑149 (MQ=255)
                  tttCTACTTCTTCGCCCGTTTGACGCTGTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTc                                                                                                                                <  4:681722/149‑1 (MQ=255)
                      tACTTCTTCGCCCGTTTGACGCTGTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGcc                                                                                                                            <  4:1624555/149‑1 (MQ=255)
                      tACTTCTTCGCCCGTTTGACGCTGTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGcc                                                                                                                            >  3:135770/1‑149 (MQ=255)
                         ttcttcGCCCGTTTGACGCTGTTTTGCGCGGCGTGGATTGCCACCGCCGAATTTAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTgg                                                                                                                         >  1:1426796/1‑149 (MQ=255)
                           cttcGCCCGTTTGACGCTGTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCt                                                                                                                       >  2:1115601/1‑149 (MQ=255)
                               gCCCGTTTGACGCTGTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATcc                                                                                                                                                 >  2:749300/1‑119 (MQ=255)
                               gCCCGTTTGACGCTGTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATcc                                                                                                                                                 <  1:749300/119‑1 (MQ=255)
                               gCCCGTTTGACGCTGTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTcccc                                                                                                                   <  3:980780/149‑1 (MQ=255)
                                   gTTTGACGCTGTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGc                                                                                                                                              <  1:1424737/118‑1 (MQ=255)
                                   gTTTGACGCTGTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAg                                                                                                               <  1:1098238/149‑1 (MQ=255)
                                       gACGCTGTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCg                                                                                                           <  1:1058332/149‑1 (MQ=255)
                                         cGCTGTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGa                                                                                                         <  1:1695316/149‑1 (MQ=255)
                                         cGCTGTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGa                                                                                                         <  3:122144/149‑1 (MQ=255)
                                         cGCTGTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATAAGGCGTTTACGCCGCATCCGGCCTatt                                                                                                                                         >  2:1405924/1‑115 (MQ=37)
                                           cTGTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATa                                                                                                       <  2:314902/149‑1 (MQ=255)
                                            tGTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCGGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATaa                                                                                                      <  3:1673773/149‑1 (MQ=255)
                                            tGTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATaa                                                                                                      >  2:507545/1‑149 (MQ=255)
                                                 tGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATgc                                                                                                 <  2:378492/149‑1 (MQ=255)
                                                 tGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATgc                                                                                                 <  1:118093/149‑1 (MQ=255)
                                                          tGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAgcatc                                                                                        <  3:1376816/149‑1 (MQ=255)
                                                          tGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATGTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAgcatc                                                                                        <  1:1364837/149‑1 (MQ=255)
                                                            gATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGAGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAgcatcgc                                                                                      >  4:71294/1‑149 (MQ=255)
                                                                gCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATcc                                                                                  <  2:1686233/149‑1 (MQ=255)
                                                                gCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATcc                                                                                  <  1:1221018/149‑1 (MQ=255)
                                                                 ccACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCg                                                                                 >  2:67219/1‑149 (MQ=255)
                                                                 ccACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCg                                                                                 >  4:372879/1‑149 (MQ=255)
                                                                  cACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCgg                                                                                >  2:1097287/1‑149 (MQ=255)
                                                                  cACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCgg                                                                                <  1:282374/149‑1 (MQ=255)
                                                                  cACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCgg                                                                                >  2:1591411/1‑149 (MQ=255)
                                                                   aCCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGc                                                                               >  1:935902/1‑149 (MQ=255)
                                                                   aCCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGc                                                                               >  4:287634/1‑149 (MQ=255)
                                                                   aCCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGc                                                                               <  2:189166/149‑1 (MQ=255)
                                                                        cgAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATaa                                                                          >  3:407155/1‑149 (MQ=255)
                                                                            tataAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATgcg                                                                      >  1:1487296/1‑149 (MQ=255)
                                                                              taAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGca                                                                    <  1:102182/149‑1 (MQ=255)
                                                                                 aGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATgc                                                                                                 >  2:1641951/1‑117 (MQ=255)
                                                                                 aGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATgc                                                                                                 <  1:1641951/117‑1 (MQ=255)
                                                                                  gacgacCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGTCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATa                                                                >  3:371252/1‑149 (MQ=255)
                                                                                   acgacCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATaa                                                               <  3:478813/149‑1 (MQ=255)
                                                                                     gacCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAAcc                                                             >  4:30353/1‑149 (MQ=255)
                                                                                     gacCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAAcc                                                             >  2:1340820/1‑149 (MQ=255)
                                                                                     gacCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAAcc                                                             >  4:832515/1‑149 (MQ=255)
                                                                                     gacCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAAcc                                                             >  4:1672545/1‑149 (MQ=255)
                                                                                        ccGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACctct                                                          >  4:1495132/1‑149 (MQ=255)
                                                                                        ccGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACctct                                                          >  1:263470/1‑149 (MQ=255)
                                                                                        ccGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACctct                                                          <  1:67219/149‑1 (MQ=255)
                                                                                        ccGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACctct                                                          >  2:213274/1‑149 (MQ=255)
                                                                                         cgcgAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACCTCtt                                                         <  3:1510642/149‑1 (MQ=255)
                                                                                         cgcgAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACCTCtt                                                         <  2:997465/149‑1 (MQ=255)
                                                                                           cgAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACCTCTTGa                                                       <  2:582575/149‑1 (MQ=255)
                                                                                             aaTGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACCTCTTGaaa                                                     <  1:359737/149‑1 (MQ=255)
                                                                                                gCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACCTCTTGAAACTc                                                  <  3:902666/149‑1 (MQ=255)
                                                                                                  cGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACCTCTTGAAACTCGt                                                >  4:222608/1‑149 (MQ=255)
                                                                                                    gggAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACCTCTTGAAACTCGTcc                                              >  1:1088335/1‑149 (MQ=255)
                                                                                                     ggAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACCTCTTGAAACTCGTccc                                             <  3:832515/149‑1 (MQ=255)
                                                                                                     ggAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACCTCTTGAAACTCGTccc                                             <  2:871708/149‑1 (MQ=255)
                                                                                                     ggAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACCTCTTGAAACTCGTccc                                             >  1:632002/1‑149 (MQ=255)
                                                                                                     ggAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACCTCTTGAAACTCGTccc                                             <  3:30353/149‑1 (MQ=255)
                                                                                                           cGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACCTCTTGAAACTCGTCCCCAGAGc                                       <  2:1404176/149‑1 (MQ=255)
                                                                                                            gCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACCTCTTGAAACTCGTCCCCAGAGcc                                      >  3:1312010/1‑149 (MQ=255)
                                                                                                                 cTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACCTCTTGAAACTCGTCCCCAGAGcc                                      <  1:929198/144‑1 (MQ=255)
                                                                                                                 cTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACCTCTTGAAACTCGTCCCCAGAGcc                                      >  2:929198/1‑144 (MQ=255)
                                                                                                                  ttAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATaa                                                                                                      >  1:1607934/1‑79 (MQ=255)
                                                                                                                  ttAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATaa                                                                                                      <  2:1607934/79‑1 (MQ=255)
                                                                                                                  ttAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACCTCTTGAAACTCGTCCCCAGAGCCTCTTCa                                <  1:1234599/149‑1 (MQ=255)
                                                                                                                     ataTAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACCTCTTGAAACTCGTCCCCAGAGCCTCTTCAGcc                             <  3:550233/147‑1 (MQ=255)
                                                                                                                       aTAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACCTCTTGaaa                                                     <  3:1470139/123‑1 (MQ=255)
                                                                                                                        tAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCa                                                                                             <  3:591953/82‑1 (MQ=255)
                                                                                                                        tAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCa                                                                                             >  4:591953/1‑82 (MQ=255)
                                                                                                                           gCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACCTCTTGAAACTCGTCCCCAGAGCCTCTTCAGCCATCTAt                       >  2:805131/1‑149 (MQ=255)
                                                                                                                           gCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACCTCTTGAAACTCGTCCCCAGAGCCTCTTCAGCCATCTAt                       <  1:28486/149‑1 (MQ=255)
                                                                                                                              ggATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACCTCTTGAAACTCGTCCCCAGAGCCTCTTCAGCCATCTATTTg                    >  2:493336/1‑149 (MQ=255)
                                                                                                                              ggATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACCTCTTGAAACTCGTCCCCAGAGCCTCTTCAGCCATCTATTTg                    <  4:407155/149‑1 (MQ=255)
                                                                                                                               gATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACCTCTTGAAACTCGTCCCCAGAGCCTCTTCAGCCATCTATTTgg                   <  2:1331025/149‑1 (MQ=255)
                                                                                                                               gATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACCTCTTGAAACTCGTCCCCAGAGCCTCTTCAGCCATCTATTTgg                   <  1:315357/149‑1 (MQ=255)
                                                                                                                               gATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACCTCTTGAAACTCGTCCCCAGAGCCTCTTCAGCCATCTATTTgg                   <  1:484912/149‑1 (MQ=255)
                                                                                                                                 tGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACCTCTTGAAACTCGTCCCCAGAGCCTCTTCAGCCATCTATTTGGGa                 <  4:904092/149‑1 (MQ=255)
                                                                                                                                 tGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACCTCTTGAAACTCGTCCCCAGAGCCTCTTCAGCCATCTATTTGGGa                 <  1:507545/149‑1 (MQ=255)
                                                                                                                                    ggcgTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACCTCTTGAAACTCGTCCCCAGAGCCTCTTCAGCCATCTATTTGGGAGCa              <  2:1426796/149‑1 (MQ=255)
                                                                                                                                      cgTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACCTCTTGAAACTCGTCCCCAGAGCCTCTTCAGCCATCTATTTGGGAGCaaa            <  2:585754/149‑1 (MQ=255)
                                                                                                                                       gTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACCTCTTGAAACTCGTCCCCAGAGCCTCTTCAGCCATCTATTTGGGAGCAAAc           >  4:1056558/1‑149 (MQ=255)
                                                                                                                                       gTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACCTCTTGAAACTCGTCCCCAGAGCCTCTTCAGCCATCTATTTGGGAGCAAAc           >  1:1648052/1‑149 (MQ=255)
                                                                                                                                        tttaCGCCGCATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACCTCTTGAAACTCGTCCCCAGAGCCTCTTCa                                <  2:24641/124‑1 (MQ=255)
                                                                                                                                          aaCGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACCTCTTGAAACTCGTCCCCAGAGCCTCTTCAGCCATCt                         >  4:1483185/1‑132 (MQ=255)
                                                                                                                                          aaCGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACCTCTTGAAACTCGTCCCCAGAGCCTCTTCAGCCATCTATTTGGGAGCAAACAAt        <  4:678001/149‑1 (MQ=255)
                                                                                                                                             gCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACCTCTTGAAACTCGTCCCCAGAGCCTCTTCAGCCATCTATTTGGGAGCAAACAATTTc     >  2:1546537/1‑149 (MQ=255)
                                                                                                                                               cTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACCTCTTGAAACTCGTCCCCAGAGCCTCTTCAGCCATCTATTTGGGAGCAAACAATTTCAt   <  3:372879/149‑1 (MQ=255)
                                                                                                                                               cTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACCTCTTGAAACTCGTCCCCAGAGCCTCTTCAGCCATCTATTTGGGAGCAAACAATTTCAt   <  2:935902/149‑1 (MQ=255)
                                                                                                                                                ttATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACCTCTTGAAACTCGTCCCCAGAGCCTCTTCAGCCATCTATTTGGGAGCAAACAATTTCAtt  >  2:1190436/1‑149 (MQ=255)
                                                                                                                                                ttATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACCTCTTGAAACTCGTCCCCAGAGCCTCTTCAGCCATCTATTTGGGAGCAAACAATTTCAtt  <  1:1402274/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                    
GCTGGGGCTGATGGCATTTTTCTACTTCTTCGCCCGTTTGACGCTGTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACCTCTTGAAACTCGTCCCCAGAGCCTCTTCAGCCATCTATTTGGGAGCAAACAATTTCATT  >  NC_000913/3674258‑3674550

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: