Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,702,837 G→C 5.5% P11R (CCG→CGG)  dppF ← dipeptide/heme ABC transporter ATPas

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,702,8370GC 5.5% 644.4 / 19.2 181P11R (CCG→CGG) dppFdipeptide/heme ABC transporter ATPas
Reads supporting (aligned to +/- strand):  ref base G (80/90);  new base C (6/4);  total (87/94)
Fisher's exact test for biased strand distribution p-value = 5.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 185 total aligned reads displayed.

ACCGCAGCCAGATTCGCCCACTACTGCCAGCGTTTTGCCACGTTCAAGGTTAAACGAAACGCCATCCAGCGCTTTAACCAGACGTTCCGGCGCGAACATGCCTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCATCATCAAGTGGGTAATGGCATTTGGACTGACGCCCGTCAGCGAGCATATTCAGCGCCGGTTCTTCAGCGCGACATCTGTCAGTGGCATAGGGGCAGCGC  >  NC_000913/3702689‑3702985
                                                                                                                                                    |                                                                                                                                                    
aCCGCAGCCAGATTCGCCCACTACTGCCAGCGTTTTGCCACGTTCAAGGTTAAACGAAACGCCATCCAGCGCTTTAACCAGACGTTCCGGCGCGAACATGCCTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCg                                                                                                                                                      <  2:70797/149‑1 (MQ=255)
aCCGCAGCCAGATTCGCCCACTACTGCCAGCGTTTTGCCACGTTCAAGGTTAAACGAAACGCCATCCAGCGCTTTAACCAGACGTTCCGGCGCGAACATGCCTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCg                                                                                                                                                      >  4:1618155/1‑149 (MQ=255)
 ccGCAGCCAGATTCGCCCACTACTGCCAGCGTTTTGCCACGTTCAAGGTTAAACGAAACGCCATCCAGCGCTTTAACCAGACGTTCCGGCGCGAACATGCCTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCgg                                                                                                                                                     <  2:754762/149‑1 (MQ=255)
   gCAGCCAGATTCGCCCACTACTGCCAGCGTTTTGCCACGTTCAAGGTTAAACGAAACGCCATCCAGCGCTTTAACCAGACGTTCCGGCGCGAACATGCCTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGgtt                                                                                                                                                   >  2:208798/1‑149 (MQ=255)
     aGCCAGATTCGCCCACTACTGCCAGCGTTTTGCCACGTTCAAGGTTAAACGAAACGCCATCCAGCGCTTTAACCAGACGTTCCGGCGCGAACATGCCTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGgttgt                                                                                                                                                 <  3:405568/149‑1 (MQ=255)
       ccAGATTCGCCCACTACTGCCAGCGTTTTGCCACGTTCAAGGTTAAACGAAACGCCATCCAGCGCTTTAACCAGACGTTCCGGCGCGAACATGCCTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGttgttg                                                                                                                                               <  2:128656/149‑1 (MQ=255)
        cAGATTCGCCCACTACTGCCAGCGTTTTGCCACGTTCAAGGTTAAACGAAACGCCATCCAGCGCTTTAACCAGACGTTCCGGCGCGAACATGCCTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGc                                                                                                                                              >  2:664682/1‑149 (MQ=255)
          gATTCGCCCACTACTGCCAGCGTTTTGCCACGTTCAAGGTTAAACGAAACGCCATCCAGCGCTTTAACCAGACGTTCCGGCGCGAACATGCCTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAACCGCTGTTGCAg                                                                                                                                            <  3:213936/149‑1 (MQ=255)
           aTTCGCCCACTACTGCCAGCGTTTTGCCACGTTCAAGGTTAAACGAAACGCCATCCAGCGCTTTAACCAGACGTTCCGGCGCGAACATGCCTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAgg                                                                                                                                           <  4:771531/149‑1 (MQ=255)
           aTTCGCCCACTACTGCCAGCGTTTTGCCACGTTCAAGGTTAAACGAAACGCCATCCAGCGCTTTAACCAGACGTTCCGGCGCGAACATGCCTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAgg                                                                                                                                           <  3:258441/149‑1 (MQ=255)
              cGCCCACTACTGCCAGCGTTTTGCCACGTTCAAGGTTAAACGAAACGCCATCCAGCGCTTTAACCAGACGTTCCGGCGCGAACATGCCTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGgttgtt                                                                                                                                                >  1:557637/1‑141 (MQ=255)
              cGCCCACTACTGCCAGCGTTTTGCCACGTTCAAGGTTAAACGAAACGCCATCCAGCGCTTTAACCAGACGTTCCGGCGCGAACATGCCTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTg                                                                                                                                        <  1:1087391/149‑1 (MQ=255)
                cccACTACTGCCAGCGTTTTGCCACGTTCAAGGTTAAACGAAACGCCATCCAGCGCTTTAACCAGACGTTCCGGCGCGAACATGCCTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGc                                                                                                                                      <  3:1256385/149‑1 (MQ=255)
                 ccACTACTGCCAGCGTTTTGCCACGTTCAAGGTTAAACGAAACGCCATCCAGCGCTTTAACCAGACGTTCCGGCGCGAACATGCCTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGcc                                                                                                                                     >  1:153532/1‑149 (MQ=255)
                     tactGCCAGCGTTTTGCCACGTTCAAGGTTAAACGAAACGCCATCCAGCGCTTTAACCAGACGTTCCGGCGCGAACATGCCTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCtt                                                                                                                                 <  3:1531260/149‑1 (MQ=255)
                           cAGCGTTTTGCCACGTTCAAGGTTAAACGAAACGCCATCCAGCGCTTTAACCAGACGTTCCGGCGCGAACATGCCTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTAc                                                                                                                           >  1:1032229/1‑149 (MQ=255)
                            aGCGTTTTGCCACGTTCAAGGTTAAACGAAACGCCATCCAGCGCTTTAACCAGACGTTCCGGCGCGAACATGCCTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACt                                                                                                                          >  2:1655000/1‑149 (MQ=255)
                              cGTTTTGCCACGTTCAAGGTTAAACGAAACGCCATCCAGCGCTTTAACCAGACGTTCCGGCGCGAACATGCCTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCa                                                                                                                        <  3:946830/149‑1 (MQ=255)
                                ttttGCCACGTTCAAGGTTAAACGAAACGCCATCCAGCGCTTTAACCAGACGTTCCGGCGCGAACATGCCTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATa                                                                                                                      <  2:1351624/149‑1 (MQ=255)
                                     ccACGTTCAAGGTTAAACGAAACGCCATCCAGCGCTTTAACCAGACGTTCCGGCGCGAACATGCCTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTa                                                                                                                            >  1:506394/1‑138 (MQ=255)
                                     ccACGTTCAAGGTTAAACGAAACGCCATCCAGCGCTTTAACCAGACGTTCCGGCGCGAACATGCCTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTa                                                                                                                            <  2:506394/138‑1 (MQ=255)
                                     ccACGTTCAAGGTTAAACGAAACGCCATCCAGCGCTTTAACCAGACGTTCCGGCGCGAACATGCCTTCCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTc                                                                                                                 >  4:1369854/1‑149 (MQ=255)
                                      cACGTTCAAGGTTAAACGAAACGCCATCCAGCGCTTTAACCAGACGTTCCGGCGCGAACATGCCTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTCg                                                                                                                >  1:380373/1‑149 (MQ=255)
                                       aCGTTCAAGGTTAAACGAAACGCCATCCAGCGCTTTAACCAGACGTTCCGGCGCGAACATGCCTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGc                                                                                                                               <  4:297658/133‑1 (MQ=255)
                                          ttCAAGGTTAAACGAAACGCCATCCAGCGCTTTAACCAGACGTTCCGGCGCGAACATGCCTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAACCGCTGTTGCAg                                                                                                                                            <  1:682728/117‑1 (MQ=255)
                                             aaGGTTAAACGAAACGCCATCCAGCGCTTTAACCAGACGTTCCGGCGCGAACATGCCTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTccc                                                                                                         <  4:48789/149‑1 (MQ=255)
                                                gTTAAACGAAACGCCATCCAGCGCTTTAACCAGACGTTCCGGCGCGAACATGCCTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGc                                                                                                      <  4:1171951/149‑1 (MQ=255)
                                                gTTAAACGAAACGCCATCCAGCGCTTTAACCAGACGTTCCGGCGCGAACATGCCTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGc                                                                                                      >  4:821982/1‑149 (MQ=255)
                                                gTTAAACGAAACGCCATCCAGCGCTTTAACCAGACGTTCCGGCGCGAACATGCCTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGc                                                                                                      >  4:18336/1‑149 (MQ=255)
                                                    aacgaaacgCCATCCAGCGCTTTAACCAGACGTTCCGGCGCGAACATGCCTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGcatca                                                                                                  >  3:899780/1‑149 (MQ=255)
                                                     acgcaacgCCATCCAGCGCTTTGACCAGACGTTCCGGCGCGAACATGCCTTTCTTCACCGGATAATGCTTTTCCAGGTCGATAGCCTGCAACAGCTGTTGTTGCAGGGTGGCCTCTTGCGTACGCATAGTGTCGGCCTCCCGGcatcat                                                                                                 >  2:1410837/1‑149 (MQ=255)
                                                     acgaaacgCCATCCAGCGCTTTAACCAGACGTTCCGGCGCGAACATGCCTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATa                                                                                                                      >  1:206049/1‑128 (MQ=255)
                                                       gaaacgCCATCCAGCGCTTTAACCAGACGTTCCGGCGCGAACATGCCTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCAtcatca                                                                                               >  4:271511/1‑149 (MQ=255)
                                                         aacgCCATCCAGCGCTTTAACCAGACGTTCCGGCGCGAACATGCCTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCATCATCAAg                                                                                             >  1:95568/1‑149 (MQ=255)
                                                            gCCATCCAGCGCTTTAACCAGACGTTCCGGCGCGAACATGCCTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCATCATCAAGTgg                                                                                          <  1:593099/149‑1 (MQ=255)
                                                               aTCCAGCGCTTTAACCAGACGTTCCGGCGCGAACATGCCTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCATCATCAAGTGGGTa                                                                                       >  3:1263679/1‑149 (MQ=255)
                                                                         ttAACCAGACGTTCCGGCGCGAACATGCCTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCg                                                                                                        <  3:234190/122‑1 (MQ=255)
                                                                         ttAACCAGACGTTCCGGCGCGAACATGCCTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCg                                                                                                        >  4:234190/1‑122 (MQ=255)
                                                                           aaCCAGACGTTCCGGCGCGAACATGCCTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCATCATCAAGTGGGTAATGGCATTTGGa                                                                           <  4:6523/149‑1 (MQ=255)
                                                                            aCCAGACGTTCCGGCGCGAACATGCCTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCATCATCAAGTGGGTAATGGCATTTGGAc                                                                          <  4:1110095/149‑1 (MQ=255)
                                                                            aCCAGACGTTCCGGCGCGAACATGCCTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCATCATCAAGTGGGTAATGGCATTTGGAc                                                                          >  4:639384/1‑149 (MQ=255)
                                                                             ccAGACGTTCCGGCGCGAACATGCCTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATa                                                                                                                      >  3:510592/1‑104 (MQ=255)
                                                                             ccAGACGTTCCGGCGCGAACATGCCTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATa                                                                                                                      <  4:510592/104‑1 (MQ=255)
                                                                             ccAAACGTTCCGGCGCGAACATGCCTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCATCATCAAGTGGGTAATGGCATTTGGACt                                                                         >  2:1314992/1‑149 (MQ=255)
                                                                              cAGACGTTCCGGCGCGAACATGCCTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCATCATCAAGTgg                                                                                          >  1:308817/1‑131 (MQ=255)
                                                                              cAGACGTTCCGGCGCGAACATGCCTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCATCATCAAGTGGGTAATGGCATTTGGACTg                                                                        >  3:141233/1‑149 (MQ=255)
                                                                               aGACGTTCCGGCGCGAACATGCCTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCATCATCAAGTGGGTAATGGCATTTGGACTGa                                                                       <  4:899780/149‑1 (MQ=255)
                                                                                 aCGTTCCGGCGCGAACATGCCTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCATCATCAAGTGGGTAATGGCATTTGGACTGACg                                                                     >  1:363551/1‑149 (MQ=255)
                                                                                    ttCCGGCGCGAACATGCCTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCATCATCAAGTGGGTAATGGCATTTGGACTGACGccc                                                                  <  2:909416/149‑1 (MQ=255)
                                                                                        ggCGCGAACATGCCTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCATCATCAAGTGGGTAATGGCATTTGGACTGACGCCCGTCa                                                              >  4:1513424/1‑149 (MQ=255)
                                                                                            cgAACATGCCTTTCTTCACCGGATAATGTTTTTTCAGTTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCATCATCAAGTGGGTAATGGCATTTGGACTGACGCCCGTCAGCGa                                                          <  3:999246/149‑1 (MQ=255)
                                                                                            cgAACATGCCTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCATCATCAAGTGGGTAATGGCATTTGGACTGACGCCCGTCAGCGa                                                          <  4:141233/149‑1 (MQ=255)
                                                                                              aaCATGCCTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCATCATCAAGTGGGTAATGGCATTTGGACTGACGCCCGTCAGCGAGc                                                        <  2:867913/149‑1 (MQ=255)
                                                                                                cATGCCTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCATCATCAAGTGGGTAATGGCATTTGGACTGACGCCCGTCAGCGAGCat                                                      <  4:1307425/149‑1 (MQ=255)
                                                                                                 aTGCCTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCATCATCAAGTGGGTAATGGCATTTGGACTGACGCCCGTCAGCGAGCata                                                     >  2:584980/1‑149 (MQ=255)
                                                                                                    ccTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCATCATCAAGTGGGTAATGGCATTTGGACTGACGCCCGTCAGCGa                                                          >  1:140560/1‑141 (MQ=255)
                                                                                                    ccTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCATCATCAAGTGGGTAATGGCATTTGGACTGACGCCCGTCAGCGa                                                          <  2:140560/141‑1 (MQ=255)
                                                                                                    ccTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCATCATCAAGTGGGTAATGGCATTTGGACTGACGCCCGTCAGCGAGCATATTc                                                  >  1:385350/1‑149 (MQ=255)
                                                                                                     cTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGc                                                                                                                               >  3:851351/1‑71 (MQ=255)
                                                                                                     cTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGc                                                                                                                               <  4:851351/71‑1 (MQ=255)
                                                                                                     cTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCATCATCAAGTGGGTAATGGCATTTGGACTGACGGGCGTCAgtc                                                           >  2:42544/1‑137 (MQ=255)
                                                                                                     cTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCATCATCAAGTGGGTAATGGCATTTGGACTGACGCCCGTCAGCGAGCATATTCa                                                 >  1:786911/1‑149 (MQ=255)
                                                                                                     cTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCATCATCAAGTGGGTAATGGCATTTGGACTGACGCCCGTCAGCGAGCATATTCa                                                 <  2:786911/149‑1 (MQ=255)
                                                                                                     cTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCATCATCAAGTGGGTAATGGCATTTGGACTGACGCCCGTCAGCGAGCATATTCa                                                 >  1:1467786/1‑149 (MQ=255)
                                                                                                     cTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCATCATCAAGTGGGTAATGGCATTTGGACTGACGCCCGTCAGCGAGCATATTCa                                                 >  1:1002561/1‑149 (MQ=255)
                                                                                                     cTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCATCATCAAGTGGGTAATGGCATTTGGACTGACGCCCGTCAGCGAGCATATTCa                                                 >  3:551250/1‑149 (MQ=255)
                                                                                                     cTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCATCATCAAGTGGGTAATGGCATTTGGACTGACGCCCGTCAGCGAGCATATTCa                                                 >  3:1674158/1‑149 (MQ=255)
                                                                                                           tcACCGGATAATGTTTTTTCAGGTCGATAGTCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCATCATCAAGTGGGTAATGGCATTTGGACTGACGCCCGTCAGCGAGCATATTCa                                                 >  3:340214/1‑143 (MQ=255)
                                                                                                               cGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCATCATCaa                                                                                              <  1:1707154/94‑1 (MQ=255)
                                                                                                               cGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCATCATCaa                                                                                              >  2:1707154/1‑94 (MQ=255)
                                                                                                               cGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCATCATCAAGTGGGTAATGGCATTTGGACTGACGCCCGTCAGCGAGCATATTCAGCGCCGGttc                                       <  2:380373/149‑1 (MQ=255)
                                                                                                               cGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCATCATCAAGTGGGTAATGGCATTTGGACTGACGCCCGTCAGCGAGCATATTCAGCGCCGGttc                                       <  1:1324647/149‑1 (MQ=255)
                                                                                                                ggATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCATCATCAAGTGGGTAATGGCATTTGGACTGACGCCCGTCAGCGAGCATATTCAGCGCCGGttct                                      >  4:558516/1‑149 (MQ=255)
                                                                                                                  aTAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCATCATCAAGTGGGTAATGGCATTTGGACTGACGCCCGTCAGCGAGCATATTCAGCGCCGGttcttc                                    <  3:182106/149‑1 (MQ=255)
                                                                                                                   tAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCATCATCAAGTGGGTAATGGCATTTGGACTGACGCCCGTCAGCGAGCATATTCAGCGCCGGTTCTTCa                                   <  1:1314992/149‑1 (MQ=255)
                                                                                                                    aaTGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCATCATCAAGTGGGTAATGGCATTTGGACTGACGCCCGTCAGCGAGCATATTCAGCGCCg                                           >  3:1395382/1‑140 (MQ=255)
                                                                                                                    aaTGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCATCATCAAGTGGGTAATGGCATTTGGACTGACGCCCGTCAGCGAGCATATTCAGCGCCg                                           <  4:1395382/140‑1 (MQ=255)
                                                                                                                     aTGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGc                                                                                                                                      <  4:678366/48‑1 (MQ=255)
                                                                                                                     aTGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGc                                                                                                                                      >  3:678366/1‑48 (MQ=255)
                                                                                                                     aTGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTATTCATAGTGTCGGCCTCCCGGCATCATCAAGTGGGTAATGGCATTTGGACTGACGCCCGTCAGCGAGCATATTCAGCGCCGGTTCTTCAgc                                 <  4:1449521/149‑1 (MQ=255)
                                                                                                                     aTGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCATCATCAAGTGGGTAATGGCATTTGGACTGACGCCCGTCAGCGAGc                                                        <  3:1169095/126‑1 (MQ=255)
                                                                                                                              cAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCATCATCAAGTGGGTAATGGCATTTGGACTGACGCCCGTCAGCGAGCATATTCAGCGCCGGTTCTTCAGCGCGACATCt                        <  4:735681/149‑1 (MQ=255)
                                                                                                                              cAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCATCATCAAGTGGGTAATGGCATTTGGACTGACGCCCGTCAGCGAGCATATTCAGCGCCGGTTCTTCAGCGCGACATCt                        <  3:689791/149‑1 (MQ=255)
                                                                                                                              cAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCATCATCAAGTGGGTAATGGCATTTGGACTGACGCCCGTCAGCGAGCATATTCAGCGCCGGTTCTTCAGCGCGACATCt                        <  1:431940/149‑1 (MQ=255)
                                                                                                                               aGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCATCATCAAGTGGGTAATGGCATTTGGACTGACGCCCGTCAGCGAGCATATTCAGCGCCGGTTCTTCAgcg                                >  2:441039/1‑140 (MQ=255)
                                                                                                                                   cGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCATCATCAAGTGGGTAATGGCATTTGGACTGACGCCCGTCAGCGAGCATATTCAGCGCCGGTTCTTCAGCGCGACATCTGTCAg                   <  2:1467786/149‑1 (MQ=255)
                                                                                                                                    gATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCATCATCAAGTGGGTAATGGCATTTGGACTGACGCCCGTCAGCGAGCATATTCAGCGCCGGTTCTTCAGCGCGACATCTGTCAGt                  >  2:102981/1‑149 (MQ=255)
                                                                                                                                      tAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCATCATCAAGTGGGTAATGGCATTTGGACTGACGCCCGTCAGCGAGCATATTCAGCGCCGGTTCTTCAGCGCGACATCTGTCAGTgg                <  3:564577/149‑1 (MQ=255)
                                                                                                                                      cacccTGCAACAACCGCTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCATCATCAAGTGGGTAATGGCATTTGGACTGACGCCCGTCAGCGAGCATATTCAGCGCCGGTTCTTCAgc                                 >  3:1037896/4‑132 (MQ=255)
                                                                                                                                       aGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCATCATCAAGTGGGTAATGGCATTTGGACTGACGCCCGTCAGCGAGCATATTCAGCGCCGGTTCTTCAGCGCGACATCTGTCAGTGGc               >  1:904248/1‑149 (MQ=255)
                                                                                                                                        gCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCATCATCAAGTGGGTAATGGCATTTGGACTGACGCCCGTCAGCGAGCATATTCAGCGCCGGTTCTTCAGCGCGACATCTGTCAGTGGCa              >  4:365964/1‑149 (MQ=255)
                                                                                                                                         ccTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCATCATCAAGTGGGTAATGGCATTTGGACTGACGCCCGTCAGCGAGCATATTCAGCGCCGGttctt                                     <  1:1383690/125‑1 (MQ=255)
                                                                                                                                         ccTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCATCATCAAGTGGGTAATGGCATTTGGACTGACGCCCGTCAGCGAGCATATTCAGCGCCGGTTCTTCAGCGCGACATCTGTCAGTGGCAt             >  2:576935/1‑149 (MQ=255)
                                                                                                                                         ccTGCAACAACCGCTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCATCATCAAGTGGGTAATGGCATTTGGACTGACGCCCGTCAGCGAGCATATTCAGCGCCGGTTCTTCAGCGCGa                             <  1:122731/133‑1 (MQ=255)
                                                                                                                                         ccTGCAACAACCGCTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCATCATCAAGTGGGTAATGGCATTTGGACTGACGCCCGTCAGCGAGCATATTCAGCGCCGGTTCTTCAGCGCGACATCTGTCAGTGGCAt             >  3:791390/1‑149 (MQ=255)
                                                                                                                                         ccTGCAACAACCGCTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTCGCCCTCCCGGCATCATCAAGTGGGTAATGGCATTTGGACTGACGCCCGTCAGCGAGCATATTCAGCGCCGGTTCTTCAGCGCGa                             >  2:122731/1‑133 (MQ=255)
                                                                                                                                          cTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCatcatc                                                                                                >  1:583349/1‑65 (MQ=255)
                                                                                                                                            gCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCATCATCAAGTGGGTAATGGCATTTGGACTGACGCCCGTCAGCGAGCATATTCAGCGCCGGTTCTTCAGCGCGACATCTGTCAGTGGCATAgg          <  1:847517/149‑1 (MQ=255)
                                                                                                                                             cAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCATCATCAAGTGGGTAATGGCATTTGGACTGACGCCCGTCAGCGAGCATATTCAGCGCCGGTTCTTCAGCGCGACATCTGTCAGTGGCATAggg         <  1:576935/149‑1 (MQ=255)
                                                                                                                                                cAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCATCATCAAGTGGGTAATGGCATTTGGACTGACGCCCGTCAGCGAGCATATTCAGCGCCGGTTCTTCAGCGCGACATCTGTCAGTGGCATAGGGGCa      <  1:1682300/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
ACCGCAGCCAGATTCGCCCACTACTGCCAGCGTTTTGCCACGTTCAAGGTTAAACGAAACGCCATCCAGCGCTTTAACCAGACGTTCCGGCGCGAACATGCCTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCATCATCAAGTGGGTAATGGCATTTGGACTGACGCCCGTCAGCGAGCATATTCAGCGCCGGTTCTTCAGCGCGACATCTGTCAGTGGCATAGGGGCAGCGC  >  NC_000913/3702689‑3702985

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: