Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,051,262 A→C 6.2% noncoding (227/245 nt) csrC → CsrC sRNA sequesters CsrA, a carbon flux regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,051,2620AC 6.2% 539.2 / 21.9 157noncoding (227/245 nt)csrCCsrC sRNA sequesters CsrA, a carbon flux regulator
Reads supporting (aligned to +/- strand):  ref base A (75/72);  new base C (7/3);  total (82/75)
Fisher's exact test for biased strand distribution p-value = 3.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.51e-01

Only 100 of 159 total aligned reads displayed.

AGGAAGACAAACGTCCGGAGACGTAATTAAACGGAAATGGAATCAACACGGATTGTTCCGGCTAAAGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTATGAAACCATCATCTTCAAACTCACGCAGCAAAGGTCATGCAAAAGCGCGTCGAAAAACACGC  >  NC_000913/4051114‑4051409
                                                                                                                                                    |                                                                                                                                                   
aGGAAGACAAACGTCCGGAGACGTAATTAAACGGAAATGGAATCAACACGGATTGTTCCGGCTAAAGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTaa                                                                                                                                                     <  4:469513/149‑1 (MQ=255)
 ggAAGACAAACGTCCGGAGACGTAATTAAACGGAAATGGAATCAACACGGATTGTTCCGGCTAAAGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAAt                                                                                                                                                    >  2:966367/1‑149 (MQ=255)
 ggAAGACAAACGTCCGGAGACGTAATTAAACGGAAATGGAATCAACACGGATTGTTCCGGCTAAAGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAAt                                                                                                                                                    <  1:1395374/149‑1 (MQ=255)
  gAAGACAAACGTCCGGAGACGTAATTAAACGGAAATGGAATCAACACGGATTGTTCCGGCTAAAGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATc                                                                                                                                                   >  2:1103662/1‑149 (MQ=255)
     gACAAACGTCCGGAGACGTAATTAAACGGAAATGGAATCAACACGGATTGTTCCGGCTAAAGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGt                                                                                                                                                <  1:1103662/149‑1 (MQ=255)
     gACAAACGTCCGGAGACGTAATTAAACGGAAATGGAATCAACACGGATTGTTCCGGCTAAAGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGt                                                                                                                                                >  4:892782/1‑149 (MQ=255)
      aCAAACGTCCGGAGACGTAATTAAACGGAAATGGAATCAACACGGATTGTTCCGGCTAAAGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTc                                                                                                                                               <  2:1384517/149‑1 (MQ=255)
      aCAAACGTCCGGAGACGTAATTAAACGGAAATGGAATCAACACGGATTGTTCCGGCTAAAGGAAAAAAAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTc                                                                                                                                               >  3:805716/1‑149 (MQ=255)
        aaaCGTCCGGAGACGTAATTAAACGGAAATGGAATCAACACGGATTGTTCCGGCTAAAGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGc                                                                                                                                             >  4:989392/1‑149 (MQ=255)
           cGTCCGGAGACGTAATTAAACGGAAATGCAATCAACACGGATTGTTCCGGCTAAAGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATTTGTCGCCtt                                                                                                                                          <  4:1450892/149‑1 (MQ=255)
                ggAGACGTAATTAAACGGAAATGGAATCAACACGGATTGTTCCGGCTAAAGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGATTACTCTGTCGCCTTTTATc                                                                                                                                     >  4:661896/1‑149 (MQ=255)
                  agaCGTAATTAAACGGAAATGGAATCAACACGGATTGTTCCGGCTAAAGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCtt                                                                                                                                   <  3:756515/149‑1 (MQ=255)
                      gTAATTAAACGGAAATGGAATCAACACGGATTGTTCCGGCTAAAGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTttgct                                                                                                                               >  1:1066797/1‑149 (MQ=255)
                      gTAATTAAACGGAAATGGAATCAACACGGATTGTTCCGGCTAAAGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTttgct                                                                                                                               >  3:717674/1‑149 (MQ=255)
                      gTAATTAAACGGAAATGGAATCAACACGGATTGTTCCGGCTAAAGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTttgct                                                                                                                               >  4:604583/1‑149 (MQ=255)
                      gTAATTAAACGGAAATGGAATCAACACGGATTGTTCCGGCTAAAGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTttgct                                                                                                                               >  4:811734/1‑149 (MQ=255)
                         aTTAAACGGAAATGGAATCAACACGGATTGTTCCGGCTAAAGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTttgcttgc                                                                                                                            >  3:1502582/1‑149 (MQ=255)
                         aTTAAACGGAAATGGAATCAACACGGATTGTTCCGGCTAAAGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTttgcttgc                                                                                                                            <  3:1662914/149‑1 (MQ=255)
                          ttAAACGGAAATGGAATCAACACGGATTGTTCCGGCTAAAGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTtgcttgct                                                                                                                           >  4:21213/1‑149 (MQ=255)
                             aaCGGAAATGGAATCAACACGGATTGTTCCGGCTAAAGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTc                                                                                                                        >  1:92269/1‑149 (MQ=255)
                              aCGGAAATGGAATCAACACGGATTGTTCCGGCTAAAGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCt                                                                                                                       <  4:451012/149‑1 (MQ=255)
                              aCGGAAATGGAATCAACACGGATTGTTCCGGCTAAAGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCt                                                                                                                       <  1:633859/149‑1 (MQ=255)
                               cGGAAATGGAATCAACACGGATTGTTCCGGCTAAAGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACACAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTg                                                                                                                      <  4:1595687/149‑1 (MQ=255)
                                ggAAATGGAATCAACACGGATTGTTCCGGCTAAAGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCttt                                                                                                                         >  3:346357/1‑145 (MQ=255)
                                  aaaTGGAATCAACACGGATTGTTCCGGCTAAAGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTa                                                                                                                   <  2:1066797/149‑1 (MQ=255)
                                      ggAATCAACACGGATTGTTCCGGCTAAAGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCt                                                                                                                       <  3:1502831/141‑1 (MQ=255)
                                      ggAATCAACACGGATTGTTCCGGCTAAAGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCt                                                                                                                       >  4:1502831/1‑141 (MQ=255)
                                      ggAATCAACACGGATTGTTCCGGCTAAAGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGagt                                                                                                               >  2:110097/1‑147 (MQ=255)
                                      ggAATCAACACGGATTGTTCCGGCTAAAGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGAtt                                                                                                               >  1:205950/1‑149 (MQ=255)
                                        aaTCAACACGGATTGTTCCGGCTAAAGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTcc                                                                                                             >  3:1474023/1‑149 (MQ=255)
                                         aTCAACACGGATTGTTCCGGCTAAAGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTttgc                                                                                                                                <  1:1486926/129‑1 (MQ=255)
                                           cAACACGGATTGTTCCGGCTAAAGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAAGAAAAAAGGCGACAGATTACTCTGTCGCCtt                                                                                                                                          >  4:1065768/1‑117 (MQ=255)
                                             acacGGATTGTTCCGGCTAAAGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCcgccgc                                                                                                        >  1:1011280/1‑149 (MQ=255)
                                               acGGATTGTTCCTGCTAAAGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCaa                                                                                                      >  3:840630/1‑149 (MQ=255)
                                               acGGATTGTTCCGGCTAAAGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCaa                                                                                                      >  2:1337118/1‑149 (MQ=255)
                                               acGGATTGTTCCGGCTAAAGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCaa                                                                                                      >  3:570515/1‑149 (MQ=255)
                                                cGGATTGTTCCGGCTAAAGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGCTTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCaaa                                                                                                     >  4:433921/1‑149 (MQ=255)
                                                cGGATTGTTCCGGCTAAAGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCaaa                                                                                                     <  1:1147078/149‑1 (MQ=255)
                                                 ggATTGTTCCGGCTAAAGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAAt                                                                                                    <  3:21213/149‑1 (MQ=255)
                                                    ttGTTCCGGCTAAAGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCta                                                                                                 <  3:1029376/149‑1 (MQ=255)
                                                     tGTTCCGGCTAAAGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCtat                                                                                                <  2:579645/149‑1 (MQ=255)
                                                           ggCTAAAGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGaa                                                                                          <  3:408253/149‑1 (MQ=255)
                                                                  ggAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGaa                                                                                          >  4:1044171/1‑142 (MQ=255)
                                                                  ggAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGaa                                                                                          <  3:1044171/142‑1 (MQ=255)
                                                                   gAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACgg                                                                                  <  3:530424/149‑1 (MQ=255)
                                                                     aaaaCAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGAAAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCt                                                                                >  4:285613/1‑149 (MQ=255)
                                                                       aaCAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTttgctt                                                                                                                              <  2:1397766/101‑1 (MQ=255)
                                                                       aaCAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTccgc                                                                                                           >  1:114943/1‑120 (MQ=255)
                                                                       aaCAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTccgc                                                                                                           <  2:114943/120‑1 (MQ=255)
                                                                         cAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTaaaa                                                                            <  4:394044/149‑1 (MQ=255)
                                                                           gggTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGa                                                                          <  4:778996/149‑1 (MQ=255)
                                                                             gtgtgtTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGc                                                                                 <  3:410975/140‑1 (MQ=255)
                                                                             gtgtgtTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACg                                                                        >  4:166057/1‑149 (MQ=255)
                                                                              tgtgtTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGa                                                                       <  3:1133609/149‑1 (MQ=255)
                                                                               gtgtTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAATGACGaa                                                                      <  2:1217412/149‑1 (MQ=255)
                                                                                tgtTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAAc                                                                     <  3:605455/149‑1 (MQ=255)
                                                                                tgtTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAAc                                                                     <  2:92269/149‑1 (MQ=255)
                                                                                      cggcCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTAt                                                               <  1:1424957/149‑1 (MQ=255)
                                                                                         ccTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTATGaa                                                            <  4:1502582/149‑1 (MQ=255)
                                                                                            gCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTgcttgctt                                                                                                                          >  4:419274/1‑84 (MQ=255)
                                                                                            gCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTgcttgctt                                                                                                                          <  3:419274/84‑1 (MQ=255)
                                                                                            gCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTATGAAAcc                                                         >  2:405504/1‑149 (MQ=255)
                                                                                              aaGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTATGAAACcat                                                       <  2:1617238/149‑1 (MQ=255)
                                                                                                ggATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTATGAAACcatca                                                     <  2:1358620/149‑1 (MQ=255)
                                                                                                  aTTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTATGAAAACatcatc                                                   <  2:1138776/149‑1 (MQ=255)
                                                                                                   ttGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTATGAAACCATCATCt                                                  >  3:1527135/1‑149 (MQ=255)
                                                                                                      tAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTcc                                                                                                             <  2:17408/87‑1 (MQ=255)
                                                                                                      tAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTcc                                                                                                             >  1:17408/1‑87 (MQ=255)
                                                                                                      tAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTATGAAACCATCATCTTCa                                               >  1:1665084/1‑149 (MQ=255)
                                                                                                         gACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCAt                                                                  >  4:519489/1‑127 (MQ=255)
                                                                                                         gACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTATGAAACCATCATCTTCAAAc                                            >  4:1172231/1‑149 (MQ=255)
                                                                                                          aCCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTATGAAACCATCATCTTCAAACt                                           >  1:1225033/1‑149 (MQ=255)
                                                                                                             cttaacgggTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGATGAACCATTATGAAACCATCATCTTCAATCTCAc                                        <  1:489667/143‑1 (MQ=255)
                                                                                                               ttAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTATGAAACCATCATCTTCAAACTCACgc                                      >  2:625888/1‑149 (MQ=255)
                                                                                                                  aGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTATGAAACCATCATCTTCAAACTCACgcagc                                   >  3:1267531/1‑149 (MQ=255)
                                                                                                                      ttATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTATGAAACCATCATCTTCAAACTCACGCAGCAAAg                               >  3:1224245/1‑149 (MQ=255)
                                                                                                                       tATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTATGAAACCATCATCTTCAAACTCACGCAGCAAAg                               >  4:850385/1‑148 (MQ=255)
                                                                                                                       tATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTATGAAACCATCATCTTCAAACTCACGCAGCAAAg                               <  3:850385/148‑1 (MQ=255)
                                                                                                                             tCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTATGAAACCATCATCTTCAAACTCACGCAGCAAAg                               <  2:1592930/142‑1 (MQ=255)
                                                                                                                             tCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTATGAAACCATCATCTTCAAACTCACGCAGCAAAg                               >  1:1592930/1‑142 (MQ=255)
                                                                                                                             tCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTATGAAACCATCATCTTCAAACTCACGCAGCAAAGGTCATGc                        >  1:1003525/1‑149 (MQ=255)
                                                                                                                             tCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTATGAAACCATCATCTTCAAACTCACGCAGCAAAGGTCATGc                        >  3:1689126/1‑149 (MQ=255)
                                                                                                                               aGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTATGAAACCATCATCTTCAAACTCACGCAGCAAAGGTCATGCaa                      >  4:1182946/1‑149 (MQ=255)
                                                                                                                                 gAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTATGAAACCATCATCTTCAAACTCACGCAGCAAAGGTCATGCaaaa                    <  3:1182946/149‑1 (MQ=255)
                                                                                                                                 gAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTATGAAACCATCATCTTCAAACTCACGCAGCAAAGGTCATGCaaaa                    <  4:539446/149‑1 (MQ=255)
                                                                                                                                    aaaaGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTATGAAACCATCATCTTCAAACTCACGCAGCaaa                                >  1:379220/1‑134 (MQ=255)
                                                                                                                                      aaGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCTGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTATGAAACCATCATCTTCAAACTCACGCAGCAAAGGTCATGCAAAAGcgcg               >  2:856704/1‑149 (MQ=255)
                                                                                                                                       aGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTATGAAACCATCATCTTCAAACTCACGCAGCAAAGGTCATGCAAAAGCGCGt              <  4:1224245/149‑1 (MQ=255)
                                                                                                                                       aGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTATGAAACCATCATCTTCAAACTCACGCAGCAAAGGTCATGCAAAAGCGCGt              <  1:966367/149‑1 (MQ=255)
                                                                                                                                        ggCGACAGAGTACTCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTATGAAACCATCATCTTCAAACTCACGCAGCAAAGGTCATGCAAAAGCGCGTc             >  4:924222/1‑149 (MQ=255)
                                                                                                                                        ggCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTATGAAACCATCATCTTCAAACTCACGCAGCAAAGGTCATGCAAAAGCGCGTc             <  1:625888/149‑1 (MQ=255)
                                                                                                                                          cGACAGATTACTCTGTCGCCTTTTTTCTGTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTATGAAACCATCATCTTCAAACTCACGCAGCAAAGGTCATGCAAAAGCGCGTCGa           >  4:1036420/1‑149 (MQ=255)
                                                                                                                                           gACAGATTACTCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTATGAAACCATCATCTTCAAACTCACGCAGCAAAGGTCATGCAAAAGCGCGTCGaa          >  2:247554/1‑149 (MQ=255)
                                                                                                                                            aCAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTATGAAACCATCATCTTCAAACTCACGCAGCAAAGGTCATGCAAAAGCGCGTCGaaa         >  1:548804/1‑149 (MQ=255)
                                                                                                                                              agagTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTATGAAACCATCATCTTCAAACTCACGCAGCAAAGGTCATGCAAAAGCGCGTCGaaaaa       <  2:205950/149‑1 (MQ=255)
                                                                                                                                               gagTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTATGAAAcc                                                         <  1:1321428/98‑1 (MQ=255)
                                                                                                                                               gagTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTATGAAACCATCATCTTCAAACTCACGCAGCAAAGGTCATGCAAAAGCGCGTCGAAAAac      <  3:1535784/149‑1 (MQ=255)
                                                                                                                                                agTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTATGAAACCATCATCTTCAAACTCACGCAGCAAAGGTCATGCAAAAGCGCGTCGAAAAaca     >  1:285699/1‑149 (MQ=255)
                                                                                                                                                  tAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTATGAAACCATCATCTTCAAACTCACGCAGCAAAGGTCATGCAAAAgc                  <  4:1331098/134‑1 (MQ=255)
                                                                                                                                                   aaTCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTATGAAACCATCATCTTCAAACTCACGCAGCAAAGGTCATGCAAAAGCGCGTCGAAAAACAcgc  <  4:570515/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
AGGAAGACAAACGTCCGGAGACGTAATTAAACGGAAATGGAATCAACACGGATTGTTCCGGCTAAAGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTATGAAACCATCATCTTCAAACTCACGCAGCAAAGGTCATGCAAAAGCGCGTCGAAAAACACGC  >  NC_000913/4051114‑4051409

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: