Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,056,531 A→G 5.1% intergenic (‑192/+94) glnL ← / ← glnA sensory histidine kinase in two‑component regulatory system with GlnG/glutamine synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,056,5310AG 5.1% 727.9 / 17.5 215intergenic (‑192/+94)glnL/glnAsensory histidine kinase in two‑component regulatory system with GlnG/glutamine synthetase
Reads supporting (aligned to +/- strand):  ref base A (104/100);  new base G (4/7);  total (108/107)
Fisher's exact test for biased strand distribution p-value = 3.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.46e-01

Only 100 of 218 total aligned reads displayed.

CTTTTTACGGATAAAAAGCGCGAAGCATCAGAGAATTGACGGAGAAAAAAGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATACGCACGCGG  >  NC_000913/4056384‑4056679
                                                                                                                                                   |                                                                                                                                                    
  ttttACGGATAAAAAGCGCGAAGCATCAGAGAATTGACGGAGAAAAAAGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATTATCACCGTGTAGGCCGGATAAGa                                                                                                                                                   <  2:1086380/149‑1 (MQ=255)
  ttttACGGATAAAAAGCGCGAAGCATCAGAGAATTGACGGAGAAAAAAGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGa                                                                                                                                                   <  4:159535/149‑1 (MQ=255)
  ttttACGGATAAAAAGCGCGAAGCATCAGAGAATTGACGGAGAAAAAAGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGa                                                                                                                                                   <  3:506439/149‑1 (MQ=255)
  ttttACGGATAAAAAGCGCGAAGCATCAGAGAATTGACGGAGAAAAAAGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGa                                                                                                                                                   <  4:1689859/149‑1 (MQ=255)
  ttttACGGATAAAAAGCGCGAAGCATCAGAGAATTGACGGAGAAAAAAGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGa                                                                                                                                                   <  2:49732/149‑1 (MQ=255)
    ttACGGATAAAAAGCGCGAAGCATCAGAGAATTGACGGAGAAAAAAGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACg                                                                                                                                                 >  1:651479/1‑149 (MQ=255)
       cGGATAAAAAGCGCGAAGCATCAGAGAATTGACGGAGAAAAAAGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCAt                                                                                                                                              <  3:1251715/149‑1 (MQ=255)
        ggATAAAAAGCGCGAAGCATCAGAGAATTGACGGAGAAAAAAGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCAtt                                                                                                                                             >  4:1371286/1‑149 (MQ=255)
        ggATAAAAAGCGCGAAGCATCAGAGAATTGACGGAGAAAAAAGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCAtt                                                                                                                                             <  4:724278/149‑1 (MQ=255)
           tAAAAAGCGCGAAGCATCAGAGAATTGACGGAGAAAAAAGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGc                                                                                                                                          >  3:79036/1‑149 (MQ=255)
           tAAAAAGCGCGAAGCATCAGAGAATTGACGGAGAAAAAAGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGc                                                                                                                                          >  1:778851/1‑149 (MQ=255)
              aaaGCGCGAAGCATCAGAGAATTGACGGAGAAAAAAGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTc                                                                                                                                       <  3:457715/149‑1 (MQ=255)
              aaaGCGCGAAGCATCAGAGAATTGACGGAGAAAAAAGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTc                                                                                                                                       <  2:714366/149‑1 (MQ=255)
              aaaGCGCGAAGCATCAGAGAATTGACGGAGAAAAAAGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTc                                                                                                                                       <  1:844050/149‑1 (MQ=255)
               aaGCGCGAAGCATCAGAGAATTGACGGAGAAAAAAGCCCATGCAGAGATGGGCTACAGATAGCTGACAAAGTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCAAATGCGTct                                                                                                                                      <  4:1214294/149‑2 (MQ=255)
                   gcgAAGCATCAGAGAATTGACGGAGAAAAAAGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATGCGACGCAAATGCGTcttatc                                                                                                                                  <  4:536249/149‑6 (MQ=255)
                   gcgAAGCATCAGAGAATTGACGGAGAAAAAAGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATGCGACGCAAATGCGTcttatc                                                                                                                                  <  4:606400/149‑6 (MQ=255)
                     gAAGCATCAGAGAATTGACGGAGAAAAAAGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATGCGACGCAAATGCGTCTTATCCg                                                                                                                                <  1:1447904/149‑1 (MQ=255)
                     gAAGCATCAGAGAATTGACGGAGAAAAAAGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCg                                                                                                                                <  4:626130/149‑1 (MQ=255)
                      aaGCCTCAGAGAATTGACGGAGAAAAAAGCCCATGCAGATATGGGCTCCAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGATCCGGCCCACCACATCATCACCGTGTAGGCCGGATGTGACGCAAATGCGTCTTATCCgg                                                                                                                               <  4:1420002/149‑1 (MQ=255)
                           tCAGAGAATTGACGGAGAAAAAAGCCCATGCAGAGATGGACTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCTTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGa                                                                                                                          >  2:1693722/1‑149 (MQ=255)
                            cAGAGAATTGACGGAGAAAAAAGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGAt                                                                                                                         <  2:227093/149‑1 (MQ=255)
                             agagAATTGACGGAGAAAAAAGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGAtg                                                                                                                        >  4:732955/1‑149 (MQ=255)
                              gagaATTGACGGAGAAAAAAGCCTATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCAttt                                                                                                                                            >  2:838967/1‑128 (MQ=255)
                              gagaATTGACGGAGAAAAAAGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGAtgt                                                                                                                       >  4:697047/1‑149 (MQ=255)
                                gaATTGACGGAGAAAAAAGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGc                                                                                                                     <  2:1126064/149‑1 (MQ=255)
                                  aTTGACGGAGAAAAAAGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCtt                                                                                                                   >  3:1240585/1‑149 (MQ=255)
                                  aTTGACGGAGAAAAAAGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCtt                                                                                                                   >  3:1020326/1‑149 (MQ=255)
                                    tGACGGAGAAAAAAGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGc                                                                                                                 >  1:1028837/1‑149 (MQ=255)
                                        ggAGAAAAAAGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGAt                                                                                                                         >  4:448382/1‑137 (MQ=255)
                                        ggAGAAAAAAGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCAcc                                                                                                             >  1:763764/1‑149 (MQ=255)
                                         gagaAAAAAGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCg                                                                                                            >  3:720072/1‑149 (MQ=255)
                                            aaaaaaGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGTCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATc                                                                                                         >  3:1167465/1‑149 (MQ=255)
                                             aaaaaGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATcc                                                                                                        <  3:128951/149‑1 (MQ=255)
                                              aaaaGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGAt                                                                                                                         <  1:99769/131‑1 (MQ=255)
                                               aaaGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCa                                                                                                      <  4:1167465/149‑1 (MQ=255)
                                               aaaGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCa                                                                                                      <  3:888498/149‑1 (MQ=255)
                                               aaaGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCa                                                                                                      <  2:1094972/149‑1 (MQ=255)
                                                aaGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAg                                                                                                     <  1:610992/149‑1 (MQ=255)
                                                  gCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATGCGACGCAAATGCGTCTTATCCGGcc                                                                                                                             <  3:631599/123‑2 (MQ=37)
                                                     cATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTg                                                                                                >  3:57989/1‑149 (MQ=255)
                                                      aTGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGc                                                                                               >  3:421545/1‑149 (MQ=255)
                                                      aTGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGc                                                                                               >  4:511028/1‑149 (MQ=255)
                                                      aTGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGc                                                                                               <  4:75548/149‑1 (MQ=255)
                                                        gCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATGCGACGCAAATGCGTcttat                                                                                                                                   <  4:1294574/111‑5 (MQ=255)
                                                        gCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATGCGACGCAAATGCGTcttat                                                                                                                                   >  3:1294574/1‑107 (MQ=255)
                                                          agagATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCaga                                                                                           >  3:587009/1‑149 (MQ=255)
                                                            agaTGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAgaga                                                                                         <  2:1266714/149‑1 (MQ=255)
                                                             gaTGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGAc                                                                                        <  3:732955/149‑1 (MQ=255)
                                                             gaTGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGAc                                                                                        <  1:530901/149‑1 (MQ=255)
                                                                gggCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAgg                                                                                     >  3:573738/1‑149 (MQ=255)
                                                                  gCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCg                                                                                   <  1:493440/149‑1 (MQ=255)
                                                                   cTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGa                                                                                  <  2:605111/149‑1 (MQ=255)
                                                                   cTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGa                                                                                  >  1:1081344/1‑149 (MQ=255)
                                                                         aTAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGtt                                                                            <  4:1400584/149‑1 (MQ=255)
                                                                              tGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCAc                                                                       <  1:310010/149‑1 (MQ=255)
                                                                               gACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGCTCCCAGGCCTGCCAGCGACAGGCGGAAAGTTTCCACg                                                                      >  4:1074148/1‑149 (MQ=255)
                                                                                      ttCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTa                                                               >  2:1553309/1‑149 (MQ=255)
                                                                                      ttCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTa                                                               >  3:287832/1‑149 (MQ=255)
                                                                                       tCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTaa                                                              <  4:1164754/149‑1 (MQ=255)
                                                                                            ttGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTaaacact                                                         >  3:379847/1‑145 (MQ=255)
                                                                                            ttGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAacac                                                         <  1:430335/149‑1 (MQ=255)
                                                                                               gagaGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGTGAAAAGTTTCTACGGCAACTAACACACTTa                                                      <  2:1028837/149‑1 (MQ=255)
                                                                                                 gagCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGa                                                    >  4:11410/1‑149 (MQ=255)
                                                                                                  agCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGAc                                                   >  1:1366536/1‑149 (MQ=255)
                                                                                                     gACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGc                                                                    >  3:1551345/1‑129 (MQ=255)
                                                                                                     gACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGc                                                                    <  4:1551345/129‑1 (MQ=255)
                                                                                                     gACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCt                                                <  4:363177/149‑1 (MQ=255)
                                                                                                     gACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCt                                                >  2:524023/1‑149 (MQ=255)
                                                                                                        tCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAacac                                                         >  1:740479/1‑137 (MQ=255)
                                                                                                        tCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAacac                                                         <  2:740479/137‑1 (MQ=255)
                                                                                                        tCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTgta                                             >  1:1004998/1‑149 (MQ=255)
                                                                                                          aCCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAg                                                                                      >  2:206789/1‑106 (MQ=255)
                                                                                                          aCCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTgtagt                                           >  1:502260/1‑149 (MQ=255)
                                                                                                          aCCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTgtagt                                           >  4:1469318/1‑149 (MQ=255)
                                                                                                          aCCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTgtagt                                           >  1:1239288/1‑149 (MQ=255)
                                                                                                           ccTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAg                                                                                                     >  3:140420/1‑90 (MQ=255)
                                                                                                            cTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTTTAGTAc                                         <  2:1500534/149‑1 (MQ=255)
                                                                                                             tGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCa                                                                                                      <  3:1143215/87‑1 (MQ=255)
                                                                                                               cTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTGTAGTACAGc                                      >  4:262725/1‑149 (MQ=255)
                                                                                                                tCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTa                                                      >  1:1146384/1‑132 (MQ=255)
                                                                                                                tCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTGGAGTACAGCt                                     >  3:364406/1‑149 (MQ=255)
                                                                                                                     ccTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAAc                                <  4:79036/149‑1 (MQ=255)
                                                                                                                       tACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAActc                              >  2:568077/1‑149 (MQ=255)
                                                                                                                       tACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAActc                              >  3:1189764/1‑149 (MQ=255)
                                                                                                                          cacaTCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTaaaa                                                            >  4:1532791/1‑116 (MQ=255)
                                                                                                                          cacaTCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTaaaa                                                            <  3:1532791/116‑1 (MQ=255)
                                                                                                                            catcatCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAg                                                                                      >  1:1693504/1‑88 (MQ=255)
                                                                                                                             atcatcACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCgg                        >  1:1614521/1‑149 (MQ=255)
                                                                                                                             atcatcACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCgg                        >  4:540081/1‑149 (MQ=255)
                                                                                                                                 tcaCCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGc                    <  3:307191/149‑1 (MQ=255)
                                                                                                                                 tcaCCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGc                    <  3:1385976/149‑1 (MQ=255)
                                                                                                                                  caCCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCg                   >  2:1171507/1‑149 (MQ=255)
                                                                                                                                       tgtAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTc              <  4:1181876/149‑1 (MQ=255)
                                                                                                                                           ggCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATac          <  1:1058184/149‑1 (MQ=255)
                                                                                                                                            gCCGGATGCGACGCAAATGCGTCTTATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATacg         >  2:441419/1‑149 (MQ=255)
                                                                                                                                            gCCGGATAAGACGCATTTGCGTCGCATCCGGCGGGATGGGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTGTGGTACAGCTCACACTCTACCGGATGCGGAGTCATaca         >  2:1654542/1‑148 (MQ=255)
                                                                                                                                                gATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATacgcacg     >  2:988394/1‑149 (MQ=255)
                                                                                                                                                gATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATacgcacg     >  2:324420/1‑149 (MQ=255)
                                                                                                                                                   aaGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATACGCACGCgg  <  4:1014679/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
CTTTTTACGGATAAAAAGCGCGAAGCATCAGAGAATTGACGGAGAAAAAAGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATACGCACGCGG  >  NC_000913/4056384‑4056679

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: