Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,056,540 T→A 6.8% intergenic (‑201/+85) glnL ← / ← glnA sensory histidine kinase in two‑component regulatory system with GlnG/glutamine synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,056,5400TA 6.8% 770.2 / 33.6 220intergenic (‑201/+85)glnL/glnAsensory histidine kinase in two‑component regulatory system with GlnG/glutamine synthetase
Reads supporting (aligned to +/- strand):  ref base T (106/99);  new base A (5/10);  total (111/109)
Fisher's exact test for biased strand distribution p-value = 1.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.88e-01

Only 100 of 227 total aligned reads displayed.

GGATAAAAAGCGCGAAGCATCAGAGAATTGACGGAGAAAAAAGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATACGCACGCGGTCATCTT  >  NC_000913/4056392‑4056686
                                                                                                                                                    |                                                                                                                                                  
ggATAAAAAGCGCGAAGCATCAGAGAATTGACGGAGAAAAAAGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCAtt                                                                                                                                                    <  4:724278/149‑1 (MQ=255)
ggATAAAAAGCGCGAAGCAGCAGAGAATTGACGGAGAAAAAAGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCAtt                                                                                                                                                    >  2:1090282/1‑149 (MQ=255)
   tAAAAAGCGCGAAGCATCAGAGAATTGACGGAGAAAAAAGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGc                                                                                                                                                 >  2:1540071/1‑149 (MQ=255)
   tAAAAAGCGCGAAGCATCAGAGAATTGACGGAGAAAAAAGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATAACCGTGTAGGCCGGATAAGACGCATTTGc                                                                                                                                                 >  4:1462288/1‑149 (MQ=255)
      aaaGCGCGAAGCATCAGAGAATTGACGGAGAAAAAAGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTc                                                                                                                                              <  1:844050/149‑1 (MQ=255)
      aaaGCGCGAAGCATCAGAGAATTGACGGAGAAAAAAGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTc                                                                                                                                              <  3:457715/149‑1 (MQ=255)
             gAAGCATCAGAGAATTGACGGAGAAAAAAGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCg                                                                                                                                       <  4:626130/149‑1 (MQ=255)
             gAAGCATCAGAGAATTGACGGAGAAAAAAGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCg                                                                                                                                       >  2:310010/1‑149 (MQ=255)
             gAAGCATCAGAGAATTGACGGAGAAAAAAGCCCATGAAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCg                                                                                                                                       >  3:1144403/1‑149 (MQ=255)
                   tCAGAGAATTGACGGAGAAAAAAGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGa                                                                                                                                 >  1:1250584/1‑149 (MQ=255)
                    cAGAGAATTGACGGAGAAAAAAGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGAt                                                                                                                                <  2:227093/149‑1 (MQ=255)
                     agagAATTGACGGAGAAAAAAGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGAtg                                                                                                                               >  3:1291774/1‑149 (MQ=255)
                     agagAATTGACGGAGAAAAAAGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGAtg                                                                                                                               >  2:530901/1‑149 (MQ=255)
                      gagaATTGACGGAGAAAAAAGCCTATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCAttt                                                                                                                                                   >  2:838967/1‑128 (MQ=255)
                         aaTTGACGGAGAAAAAAGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCt                                                                                                                           <  4:743421/149‑1 (MQ=255)
                          aTTGACGGAGAAAAAAGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCtt                                                                                                                          >  3:1020326/1‑149 (MQ=255)
                            tGACGGAGAAAAAAGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGc                                                                                                                        >  1:1028837/1‑149 (MQ=255)
                              aCGGAGAAAAAAGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCa                                                                                                                      <  2:183026/149‑1 (MQ=255)
                                ggAGAAAAAAGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGAt                                                                                                                                >  4:448382/1‑137 (MQ=255)
                                ggAGAAAAAAGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCAcc                                                                                                                    >  1:763764/1‑149 (MQ=255)
                                ggAGAAAAAAGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCAcc                                                                                                                    >  2:512859/1‑149 (MQ=255)
                                 gagaAAAAAGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCg                                                                                                                   <  4:1074668/149‑1 (MQ=255)
                                   gaAAAAAGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGAt                                                                                                                 >  3:372386/1‑149 (MQ=255)
                                      aaaaGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGAt                                                                                                                                <  1:99769/131‑1 (MQ=255)
                                      aaaaGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATccc                                                                                                              >  4:511884/1‑149 (MQ=255)
                                      aaaaGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATccc                                                                                                              >  3:1303306/1‑149 (MQ=255)
                                       aaaGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCa                                                                                                             <  4:1167465/149‑1 (MQ=255)
                                       aaaGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCa                                                                                                             <  3:888498/149‑1 (MQ=255)
                                        aaGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAg                                                                                                            <  4:720072/149‑1 (MQ=255)
                                          gCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATGCGACGCAAATGCGTCTTATCCGGcc                                                                                                                                    <  3:631599/123‑2 (MQ=37)
                                             cATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTg                                                                                                       >  3:57989/1‑149 (MQ=255)
                                              aTGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGc                                                                                                      >  3:421545/1‑149 (MQ=255)
                                              aTGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGc                                                                                                      <  1:649394/149‑1 (MQ=255)
                                              aTGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCAGCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGc                                                                                                      <  4:1595324/149‑1 (MQ=255)
                                                gCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCAAATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCa                                                                                                    >  3:1173987/1‑149 (MQ=255)
                                                  agagATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCaga                                                                                                  >  3:587009/1‑149 (MQ=255)
                                                    agaTGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAgaga                                                                                                <  1:497292/149‑1 (MQ=255)
                                                    agaTGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAgaga                                                                                                >  3:1139098/1‑149 (MQ=255)
                                                    agaTGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAgaga                                                                                                <  2:1266714/149‑1 (MQ=255)
                                                     gaTGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGAc                                                                                               <  4:538833/149‑1 (MQ=255)
                                                       tGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAg                                                                                             <  4:1360319/149‑1 (MQ=255)
                                                        gggCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAgg                                                                                            >  3:573738/1‑149 (MQ=255)
                                                           cTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGa                                                                                         >  1:1081344/1‑149 (MQ=255)
                                                             aCAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGaaa                                                                                       <  1:1218880/149‑1 (MQ=255)
                                                              cAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGaaaa                                                                                      <  4:681070/149‑1 (MQ=255)
                                                                   aGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTc                                                                                 <  4:1139098/149‑1 (MQ=255)
                                                                      tGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCAc                                                                              <  1:310010/149‑1 (MQ=255)
                                                                          aaaCTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCa                                                                          >  1:567800/1‑149 (MQ=255)
                                                                            aCTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAAc                                                                        <  4:859263/149‑1 (MQ=255)
                                                                              ttCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTa                                                                      >  2:719483/1‑149 (MQ=255)
                                                                              ttCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTa                                                                      >  3:287832/1‑149 (MQ=255)
                                                                                 aCGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTaaaa                                                                   <  3:1191092/149‑1 (MQ=255)
                                                                                 aCGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTaaaa                                                                   <  4:1020326/149‑1 (MQ=255)
                                                                                 aCGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTaaaa                                                                   <  3:1667874/149‑1 (MQ=255)
                                                                                    ttGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAacac                                                                <  1:430335/149‑1 (MQ=255)
                                                                                       gagaGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGTGAAAAGTTTCTACGGCAACTAACACACTTa                                                             <  2:1028837/149‑1 (MQ=255)
                                                                                         gagCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGa                                                           >  3:363177/1‑149 (MQ=255)
                                                                                             gACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCt                                                       <  4:363177/149‑1 (MQ=255)
                                                                                              aCTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTg                                                      >  4:249205/1‑149 (MQ=255)
                                                                                                tCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAacac                                                                >  1:740479/1‑137 (MQ=255)
                                                                                                tCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTgta                                                    >  4:1541004/1‑149 (MQ=255)
                                                                                                tCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTgta                                                    >  1:1004998/1‑149 (MQ=255)
                                                                                                  aCCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAg                                                                                             >  2:206789/1‑106 (MQ=255)
                                                                                                  aCCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTgtagt                                                  >  1:1402362/1‑149 (MQ=255)
                                                                                                   ccTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAg                                                                                                            <  4:140420/90‑1 (MQ=255)
                                                                                                   ccTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTgtagta                                                 >  4:786610/1‑149 (MQ=255)
                                                                                                    cTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTGTAGTAc                                                >  3:1389754/1‑149 (MQ=255)
                                                                                                     tGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCa                                                                                                             >  4:1143215/1‑87 (MQ=255)
                                                                                                        tCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTa                                                             <  2:1146384/132‑1 (MQ=255)
                                                                                                            gCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCaaa                                        >  2:601129/1‑149 (MQ=255)
                                                                                                                aCCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAActct                                    <  3:786610/149‑1 (MQ=255)
                                                                                                                 ccACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGaaaa                                                                                      >  2:750157/1‑98 (MQ=255)
                                                                                                                  cacaTCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTaaaa                                                                   <  3:1532791/116‑1 (MQ=255)
                                                                                                                  cacaTCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTaaaa                                                                   >  4:1532791/1‑116 (MQ=255)
                                                                                                                    catcatCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAg                                                                                             >  1:1693504/1‑88 (MQ=255)
                                                                                                                     atcatcACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCgg                               >  1:1614521/1‑149 (MQ=255)
                                                                                                                       catcaCCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGAt                             <  4:1240585/149‑1 (MQ=255)
                                                                                                                        atcaCCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATg                            >  2:1466022/1‑149 (MQ=255)
                                                                                                                         tcaCCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGc                           >  1:512140/1‑149 (MQ=255)
                                                                                                                         tcaCCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGc                           >  4:1385976/1‑149 (MQ=255)
                                                                                                                          caCCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTGTAGGACAGCTCAAACTCTACCGGATGCg                          >  2:1119896/1‑149 (MQ=255)
                                                                                                                           aCCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCgg                         >  2:692381/1‑149 (MQ=255)
                                                                                                                            ccGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGa                        <  1:684892/149‑1 (MQ=255)
                                                                                                                             cGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAg                       <  3:728170/149‑1 (MQ=255)
                                                                                                                               tgtAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTc                     <  4:1181876/149‑1 (MQ=255)
                                                                                                                                   ggCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATac                 <  1:1058184/149‑1 (MQ=255)
                                                                                                                                   ggCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATac                 <  3:1279589/149‑1 (MQ=255)
                                                                                                                                    gCCGGATGCGACGCAAATGCGTCTTATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATacg                >  2:441419/1‑149 (MQ=255)
                                                                                                                                    gCCGGATAAGACGCATTTGCGTCGCATCCGGCGGGATGGGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTGTGGTACAGCTCACACTCTACCGGATGCGGAGTCATaca                >  2:1654542/1‑148 (MQ=255)
                                                                                                                                        gATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATacgcacg            >  2:988394/1‑149 (MQ=255)
                                                                                                                                           gcgACGCAAATGCGTCTTATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGc                                                                                           <  1:800585/65‑1 (MQ=25)
                                                                                                                                            aGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATACGCACGCGGt        <  3:1541004/149‑1 (MQ=255)
                                                                                                                                             gACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGaaaa                                                                                      <  4:582039/70‑1 (MQ=255)
                                                                                                                                             gACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATACGCACGCGGTc       >  4:542713/1‑149 (MQ=255)
                                                                                                                                             gACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATACGCACGCGGTc       >  3:636579/1‑149 (MQ=255)
                                                                                                                                             gACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATACGCACGCGGTc       >  3:873369/1‑149 (MQ=255)
                                                                                                                                              aCGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATACGCACGCGGTCa      >  1:1346919/1‑149 (MQ=255)
                                                                                                                                              aCGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATACGCACGCGGTCa      >  4:373125/1‑149 (MQ=255)
                                                                                                                                              aCGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATACGCAAGCGGTCa      <  3:697047/149‑1 (MQ=255)
                                                                                                                                                gCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATACGCACGCGGTCAtc    <  3:542713/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
GGATAAAAAGCGCGAAGCATCAGAGAATTGACGGAGAAAAAAGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATACGCACGCGGTCATCTT  >  NC_000913/4056392‑4056686

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: