Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,070,413 T→G 8.0% intergenic (‑12/+102) yihR ← / ← yihS putative sulphoquinovose mutarotase/sulphoquinovose isomerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,070,4130TG 8.0% 599.8 / 26.9 184intergenic (‑12/+102)yihR/yihSputative sulphoquinovose mutarotase/sulphoquinovose isomerase
Reads supporting (aligned to +/- strand):  ref base T (91/77);  new base G (6/10);  total (97/87)
Fisher's exact test for biased strand distribution p-value = 2.95e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 186 total aligned reads displayed.

CCTGAAAGGTTAATTCAGCCAGACCAGCACCGACGGTCACAATGGTCGCGTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCC  >  NC_000913/4070266‑4070560
                                                                                                                                                   |                                                                                                                                                     
   gAAAGGTTAATTCAGCCAGACCAGCACCGACGGTCACAATGGTCGCGTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAgg                                                                                                                                                   >  4:831560/1‑149 (MQ=255)
    aaaGGTTAATTCAGCCAGACCAGCACCGACGGTCACAATGGTCGCGTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGc                                                                                                                                                  <  4:1439842/149‑1 (MQ=255)
       ggTTAATTCAGCCAGACCAGCACCGACGGTCACAATGGTCGCGTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGa                                                                                                                                              <  3:94368/149‑1 (MQ=255)
       ggTTAATTCAGCCAGACCAGCACCGACGGTCACAATGGTCGCGTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGa                                                                                                                                              <  3:1566368/149‑1 (MQ=255)
        gTTAATTCAGCCAGACCAGCACCGACGGTCACAATGGTCGCGTGGTAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGAt                                                                                                                                             <  3:1404626/149‑1 (MQ=255)
          tAATTCAGCCAGACCAGCACCGACGGTCACAATGGTCGCGTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATCAGCCTCACGGGAGGCTgtatgt                                                                                                                                           <  3:762131/149‑6 (MQ=255)
           aaTTCAGCCAGACCAGCACCGACGGTCACAATGGTCGCGTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTc                                                                                                                                          <  1:849699/149‑1 (MQ=255)
           aaTTCAGCCAGACCAGCACCGACGGTCACAATGGTCGCGTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTc                                                                                                                                          <  2:796074/149‑1 (MQ=255)
             ttCAGCCAGACCAGCACCGACGGTCACAATGGTCGCGTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCaa                                                                                                                                        >  3:151348/1‑149 (MQ=255)
              tCAGCCAGACCAGCACCGACGGTCACAATGGTCGCGTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATCAGCCTCACGGGAGGCTGTATGTcgt                                                                                                                                        >  4:865834/1‑146 (MQ=255)
                 gCCAGACCAGCACCGACGGTCACAATGGTCGCGTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAAccc                                                                                                                                    >  1:836428/1‑149 (MQ=255)
                 gCCAGACCAGCACCGACGGTCACAATGGTCGCGTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAAccc                                                                                                                                    <  3:1356608/149‑1 (MQ=255)
                        cAGCACCGACGGTCACAATGGTCGCGTGATAATCCCCCGCTGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACt                                                                                                                            >  1:499518/1‑149 (MQ=255)
                        cAGCACCGACGGTCACAATGGTCGCGTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACt                                                                                                                            >  3:282198/1‑149 (MQ=255)
                        cAGCACCGACGGTCACAATGGTCGCGTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACt                                                                                                                            >  4:706739/1‑149 (MQ=255)
                         aGCACCGACGGTCACAATGGTCGCGTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTg                                                                                                                           <  1:1181157/149‑1 (MQ=255)
                          gCACCGACGGTCACAATGGTCGCGTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGc                                                                                                                          >  3:1549156/1‑149 (MQ=255)
                          gCACCGACGGTCACAATGGTCGCGTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGc                                                                                                                          <  3:434415/149‑1 (MQ=255)
                                aCGGTCACAATGGTCGCGTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGa                                                                                                                    <  2:1261720/149‑1 (MQ=255)
                                    tCACAATGGTCGCGTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGt                                                                                                                >  1:19197/1‑149 (MQ=255)
                                        aaTGGTCGCGTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGa                                                                                                                    >  2:1069557/1‑141 (MQ=255)
                                        aaTGGTCGCGTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGa                                                                                                                    <  1:1069557/141‑1 (MQ=255)
                                         aTGGTCGCGTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCt                                                                                                           >  4:387165/1‑149 (MQ=255)
                                          tGGTCGCGTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTc                                                                                                          <  2:1500650/149‑1 (MQ=255)
                                            gTCGCGTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGc                                                                                                        <  2:966979/149‑1 (MQ=255)
                                               gcgTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGa                                                                                                                                                     >  3:862895/1‑103 (MQ=255)
                                                 gTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCtt                                                                                                   >  1:1377408/1‑149 (MQ=255)
                                                  tGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCttt                                                                                                  <  4:457215/149‑1 (MQ=255)
                                                  tGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCttt                                                                                                  >  2:1050379/1‑149 (MQ=255)
                                                  tGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCttt                                                                                                  <  2:143725/149‑1 (MQ=255)
                                                     tAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTggg                                                                                               <  2:1663678/149‑1 (MQ=255)
                                                     tAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTggg                                                                                               <  3:1579397/149‑1 (MQ=255)
                                                      aaTCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTgggg                                                                                              >  2:388532/1‑149 (MQ=255)
                                                        tCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGga                                                                                             <  3:526237/148‑1 (MQ=255)
                                                         cccccGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGgaga                                                                                           <  2:1572296/149‑1 (MQ=255)
                                                         cccccGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGgaga                                                                                           <  1:779516/149‑1 (MQ=255)
                                                         cccccGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGgaga                                                                                           <  4:154071/149‑1 (MQ=255)
                                                          ccccGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAAGGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGagag                                                                                          >  2:427042/1‑149 (MQ=255)
                                                          ccccGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCACAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGagag                                                                                          <  4:1075150/149‑1 (MQ=255)
                                                          ccccGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGagag                                                                                          >  4:1260580/1‑149 (MQ=255)
                                                           cccGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAgg                                                                                         >  4:1145133/1‑149 (MQ=255)
                                                           cccGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAgg                                                                                         >  1:1465397/1‑149 (MQ=255)
                                                           cccGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAgg                                                                                         >  3:1462289/1‑149 (MQ=255)
                                                           cccGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAgg                                                                                         <  1:427042/149‑1 (MQ=255)
                                                           cccGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAgg                                                                                         >  3:780058/1‑149 (MQ=255)
                                                            ccGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAggg                                                                                        >  2:814519/1‑149 (MQ=255)
                                                            ccGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTACCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAggg                                                                                        >  4:1348282/1‑149 (MQ=255)
                                                               cgGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTa                                                                                     <  3:387165/149‑1 (MQ=255)
                                                                ggCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAg                                                                                    <  1:990195/149‑1 (MQ=255)
                                                                   aaGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGga                                                                                 <  2:851543/149‑1 (MQ=255)
                                                                   aaGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGga                                                                                 >  3:1616708/1‑149 (MQ=255)
                                                                       gAAACTGTCTGTCCACTGCAATGCATATTGGTGATTAGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGGCCCTTTGGGGAGAGGGTTAGGGAGAgg                                                                             <  4:282166/149‑1 (MQ=255)
                                                                       gAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTgg                                                                                                >  1:1144312/1‑130 (MQ=255)
                                                                       gAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTgg                                                                                                <  2:1144312/130‑1 (MQ=255)
                                                                       gAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAgg                                                                             <  2:1418600/149‑1 (MQ=255)
                                                                       gAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAgg                                                                             <  3:1260580/149‑1 (MQ=255)
                                                                          gcGGTCTGCCCGCGCCAATGCAAATGGGGGATTTGCATACAAGGCACCTAAATTAACGCCATACAGCCTCCCGGGAGGCTGATGTAAAACCCAAAAACTGCACCGGACGGCCCCCTCGCCCCTTTGGGGAGAGGGTGAGGGAGAGGGGa                                                                          <  4:1549156/148‑1 (MQ=255)
                                                                              tCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAAc                                                                      >  1:309831/1‑149 (MQ=255)
                                                                                  tCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAggg                                                                            <  4:1570552/139‑1 (MQ=255)
                                                                                     aCTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTggg                                                                                               >  4:1602388/1‑117 (MQ=255)
                                                                                            tGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTAtttt                                                        >  4:545243/1‑149 (MQ=255)
                                                                                              cATATTGTTGATTTGCATACAAGGCCCATTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAACCTGCACCGGACGGCCCCCTCGCCCCTTTGGGGGGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCa                                                      <  3:1599614/149‑1 (MQ=39)
                                                                                              cATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCa                                                      >  3:334392/1‑149 (MQ=255)
                                                                                              cATATTGGTGATTTGCAAACAAGGCACCTTAATTAACAACATACCGCCTCCCGGGAGGCTGATGCCAAACCCAAAAACTGCCCCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCa                                                      <  3:1395246/149‑1 (MQ=38)
                                                                                              cATATTGGGGATTTGCAAACAAGGCACCTAAATTAACGACACACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGCCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCa                                                      <  4:1345926/149‑1 (MQ=39)
                                                                                                  ttGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATc                                                  >  3:75216/1‑149 (MQ=255)
                                                                                                   tGGTGATTTGCATACAAGGCACCTTAATTAACGACATACACCCCCCCGGGGGGCTGATGTAAAACCCAAAAACCGCCCCGGACGGTCCCCCCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCa                                                 <  1:463657/149‑1 (MQ=38)
                                                                                                     gTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGt                                               >  2:757405/1‑149 (MQ=255)
                                                                                                      tGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTa                                             >  2:58711/1‑149 (MQ=255)
                                                                                                        aTTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTccc                                                                                                             <  1:142270/84‑1 (MQ=255)
                                                                                                            gCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCtt                                                                                                   <  4:1687184/90‑1 (MQ=255)
                                                                                                              aTACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCa                                      <  3:1649199/149‑1 (MQ=255)
                                                                                                              aTACAAGGCACCTTAATTAACAACATACAGCCTCCCGTGTGGGTGATGTCAAACCGAAAAGCTGCACAGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCa                                      <  3:173649/149‑1 (MQ=38)
                                                                                                                aCAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGccc                                                                                                      <  4:758069/83‑1 (MQ=255)
                                                                                                                 cAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTa                                   >  4:646457/1‑149 (MQ=255)
                                                                                                                       aCCTTAATTAACGACATACAGCCTCCTGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATcc                             >  2:1286598/1‑149 (MQ=255)
                                                                                                                       aCCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATcc                             >  4:1633567/1‑149 (MQ=255)
                                                                                                                        ccTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATcc                             >  1:694804/1‑149 (MQ=255)
                                                                                                                         cTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCcag                           <  4:85663/149‑1 (MQ=255)
                                                                                                                         cTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCcag                           >  1:1399647/1‑149 (MQ=255)
                                                                                                                         cTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCcag                           >  1:1634505/1‑149 (MQ=255)
                                                                                                                         cTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCcag                           >  2:1326232/1‑149 (MQ=255)
                                                                                                                         cTTAATTAACGACATACAGCCCCCCGGGAGGCTGATGTCAAACCCAAAACCTGCCCCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCcag                           <  3:706739/149‑1 (MQ=255)
                                                                                                                            aattaaCGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCcagcag                        >  1:1299457/1‑149 (MQ=255)
                                                                                                                             attaaCGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACGGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGa                       <  4:593428/149‑1 (MQ=255)
                                                                                                                              ttaaCGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGAc                      >  2:730652/1‑149 (MQ=255)
                                                                                                                              gtaaCGACATACAGCCGCCCTTGAGGCTGATGTCAAACCCAAAAACTGCAACGGACGGGCACCTTGCCCCTTTGGGGAGAGGCTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCATTTATTTCGCGTTAATGTCCAGCAGAc                      >  2:215713/2‑149 (MQ=255)
                                                                                                                                 aCGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCg                   >  1:576399/1‑149 (MQ=255)
                                                                                                                                   gACATCCCGCCTCCCGTGAGGCTGATGTCAAACCCAAAACCTGCACCGGACGGTCCCCTCGCCCTTTTGGGGAGAGGGTTGGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCt                 <  2:1634505/149‑1 (MQ=255)
                                                                                                                                      aTACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCa              >  1:530781/1‑149 (MQ=255)
                                                                                                                                       tACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTc                                                                                                               <  1:1367300/51‑1 (MQ=255)
                                                                                                                                       atcAGCCTCACGGGAGGCTGTATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCa              >  3:1430324/3‑149 (MQ=39)
                                                                                                                                       atcAGCCTCACGGGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATcc                             >  4:412314/3‑133 (MQ=255)
                                                                                                                                       atcAGCCTCACGGGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATcc                             <  3:412314/131‑1 (MQ=255)
                                                                                                                                       atcAGCCTCACGGGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCaa             >  2:541496/3‑149 (MQ=255)
                                                                                                                                           gCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGc         <  3:566144/149‑1 (MQ=255)
                                                                                                                                            ccTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCt        <  4:28758/149‑1 (MQ=255)
                                                                                                                                              tCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTgg      >  1:1204140/1‑149 (MQ=255)
                                                                                                                                               cccGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGa     <  2:1374752/149‑1 (MQ=255)
                                                                                                                                                   tGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGcc  >  1:1008682/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
CCTGAAAGGTTAATTCAGCCAGACCAGCACCGACGGTCACAATGGTCGCGTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCC  >  NC_000913/4070266‑4070560

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: