Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,082,696 G→C 6.6% Q22E (CAG→GAG)  fdoH ← formate dehydrogenase‑O, Fe‑S subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,082,6960GC 6.6% 862.6 / 38.2 243Q22E (CAG→GAG) fdoHformate dehydrogenase‑O, Fe‑S subunit
Reads supporting (aligned to +/- strand):  ref base G (111/116);  new base C (10/6);  total (121/122)
Fisher's exact test for biased strand distribution p-value = 3.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

Only 100 of 245 total aligned reads displayed.

GGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCGCATCACCAACGAATGGC  >  NC_000913/4082555‑4082844
                                                                                                                                             |                                                                                                                                                    
   tGTCGGACACCCCAATGTTATTGCCGGCGGCATGGCGAATGTCGTTCCACTCTCAACACGCCACCTCACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTGCCGCGGCTGAGGCGAgggg                                                                                                                                            <  4:763977/149‑1 (MQ=25)
    gTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCggggg                                                                                                                                           <  4:708658/149‑1 (MQ=255)
    gTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGCCCCGCGCCTCAGGCGCgggac                                                                                                                                           <  1:76432/149‑3 (MQ=255)
       gTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTCAg                                                                                                                                                    <  1:434906/137‑1 (MQ=255)
         acacCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGa                                                                                                                                      >  3:1615877/1‑149 (MQ=255)
             cccAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCa                                                                                                                                        <  2:358648/143‑1 (MQ=255)
             cccAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGt                                                                                                                                  <  2:969780/149‑1 (MQ=255)
              ccAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGtt                                                                                                                                 >  3:283482/1‑149 (MQ=255)
              ccAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGtt                                                                                                                                 >  2:313042/1‑149 (MQ=255)
               cAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTa                                                                                                                                >  3:1409520/1‑149 (MQ=255)
                aaTGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAg                                                                                                                               >  2:1567589/1‑149 (MQ=255)
                  tGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTc                                                                                                                             <  3:1209978/149‑1 (MQ=255)
                    ttATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTcgc                                                                                                                           >  4:862908/1‑149 (MQ=255)
                    ttATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTcgc                                                                                                                           >  4:1384220/1‑149 (MQ=255)
                    ttATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTcgc                                                                                                                           <  2:361763/149‑1 (MQ=255)
                    ttATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTcgc                                                                                                                           >  1:156677/1‑149 (MQ=255)
                         gCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCGACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAac                                                                                                                      >  3:1597623/1‑149 (MQ=255)
                           cGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAacga                                                                                                                    <  1:331522/149‑1 (MQ=255)
                               ggTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGaa                                                                                                                <  2:1682151/149‑1 (MQ=255)
                                gTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAAt                                                                                                               <  3:51698/149‑1 (MQ=255)
                                 tATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATg                                                                                                              <  3:581280/149‑1 (MQ=255)
                                   tCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAacgac                                                                                                                   >  2:639538/1‑142 (MQ=255)
                                       gAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCt                                                                                                        <  1:268322/149‑1 (MQ=255)
                                        aaTGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCtt                                                                                                       <  1:1354511/149‑1 (MQ=255)
                                          tGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTga                                                                                                     <  3:433899/149‑1 (MQ=255)
                                           gTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTgag                                                                                                    >  3:207537/1‑149 (MQ=255)
                                            tCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTgaga                                                                                                   <  3:17300/149‑1 (MQ=255)
                                              gTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGAtt                                                                                                 <  4:1501816/149‑1 (MQ=255)
                                                tCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGa                                                                                               >  1:541994/1‑149 (MQ=255)
                                                   aCTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATaa                                                                                            >  3:857610/1‑149 (MQ=255)
                                                     tctGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGc                                                                                          <  1:1381684/149‑1 (MQ=255)
                                                       tGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCa                                                                                        >  2:531911/1‑149 (MQ=255)
                                                         aaCACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCata                                                                                      >  4:224429/1‑149 (MQ=255)
                                                          acacGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGTGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCatat                                                                                     >  2:281118/1‑149 (MQ=255)
                                                          acacGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGGACGACGAATGATATCTTGAGATTGATAAGCCatat                                                                                     <  1:1157023/149‑1 (MQ=255)
                                                          acacGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCatat                                                                                     <  1:1044273/149‑1 (MQ=255)
                                                           cacGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATg                                                                                    >  4:1688000/1‑149 (MQ=255)
                                                            acGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAacgac                                                                                                                   <  2:880273/117‑1 (MQ=255)
                                                            acGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTga                                                                                                     <  4:523099/131‑1 (MQ=255)
                                                             cGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATgtc                                                                                  <  3:1507673/149‑1 (MQ=255)
                                                             cGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATgtc                                                                                  <  3:1319836/149‑1 (MQ=255)
                                                              gCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATgtcg                                                                                 >  4:177621/1‑149 (MQ=255)
                                                                 aCCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCggg                                                                                                                                             <  4:1437498/86‑1 (MQ=255)
                                                                 aCCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAac                                                                                                                      <  4:1515021/109‑1 (MQ=255)
                                                                  ccTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGtctc                                                                             <  2:801216/149‑1 (MQ=255)
                                                                    tGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTcgc                                                                                                                           <  3:1336754/101‑1 (MQ=255)
                                                                    tGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTcgc                                                                                                                           >  4:1336754/1‑101 (MQ=255)
                                                                     gACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGt                                                                          >  2:697991/1‑149 (MQ=255)
                                                                      aCAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGtt                                                                         <  1:1660195/149‑1 (MQ=255)
                                                                      aCAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGCCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGtt                                                                         >  4:1120718/1‑149 (MQ=255)
                                                                       tcggCTGTAAAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTa                                                                        >  3:385832/3‑149 (MQ=255)
                                                                        aGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTac                                                                       <  2:1546601/149‑1 (MQ=255)
                                                                          gCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGt                                                                          <  1:598342/144‑1 (MQ=255)
                                                                          gCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTacac                                                                     >  3:947116/1‑149 (MQ=255)
                                                                            tttACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCt                                                                   >  3:1625537/1‑149 (MQ=255)
                                                                              tACAGCCGATACAGGTTGTAACGTCGATGATTTTCGCCACTTCTGCCTGGAAGTTCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACTAATGATATCTTGAGATTAATAAGCCATATGTCGTCTCCGTTAAACCttt                                                                 <  2:1487938/149‑1 (MQ=255)
                                                                                cAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTc                                                               >  2:15315/1‑149 (MQ=255)
                                                                                cAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTc                                                               <  1:1506686/149‑1 (MQ=255)
                                                                                cAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTc                                                               >  3:268613/1‑149 (MQ=255)
                                                                                   ccGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCata                                                                                      >  1:1392752/1‑123 (MQ=255)
                                                                                   ccGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCcac                                                            <  2:1447680/149‑1 (MQ=255)
                                                                                    cGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCcaca                                                           <  4:1247830/149‑1 (MQ=255)
                                                                                     gATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACAt                                                          >  4:480171/1‑149 (MQ=255)
                                                                                         cAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTcac                                                      <  2:1060696/149‑1 (MQ=255)
                                                                                               gtAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACaaggaa                                                >  3:835156/1‑149 (MQ=255)
                                                                                               gtAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCCCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGtct                                                                              <  4:117455/119‑1 (MQ=255)
                                                                                                tAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACaaggaag                                               <  3:1384220/149‑1 (MQ=255)
                                                                                                 aaCGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATgtc                                                                                  <  1:58278/113‑1 (MQ=255)
                                                                                                 aaCGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATgtc                                                                                  >  2:58278/1‑113 (MQ=255)
                                                                                                     tCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACtt                                          <  4:256482/149‑1 (MQ=255)
                                                                                                     tCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACtt                                          <  4:283482/149‑1 (MQ=255)
                                                                                                       gATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGCCTTaa                                        >  1:23844/1‑149 (MQ=255)
                                                                                                       gATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTaa                                        >  2:932569/1‑149 (MQ=255)
                                                                                                             tttCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCg                                  <  2:1291943/149‑1 (MQ=255)
                                                                                                                  ccACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTc                             <  4:602572/149‑1 (MQ=255)
                                                                                                                  ccACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTc                             >  2:503088/1‑149 (MQ=255)
                                                                                                                     cttcttCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGc                          <  1:898991/149‑1 (MQ=255)
                                                                                                                     cttcttCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGc                          >  1:1702627/1‑149 (MQ=255)
                                                                                                                     cttcttCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGc                          <  4:1137372/149‑1 (MQ=255)
                                                                                                                      ttcttcCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCg                         >  1:272177/1‑149 (MQ=255)
                                                                                                                       tcttcCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCgt                        <  1:932569/149‑1 (MQ=255)
                                                                                                                         ttcCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATgtcgtc                                                                               <  3:870377/92‑1 (MQ=255)
                                                                                                                           ccTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTc                    <  3:1150369/149‑1 (MQ=255)
                                                                                                                            cTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGata                                                                                                           <  2:241162/61‑1 (MQ=255)
                                                                                                                            cTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCatat                                                                                     >  2:1379535/1‑83 (MQ=255)
                                                                                                                             tGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCGc                  <  4:430808/149‑1 (MQ=255)
                                                                                                                             tGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCGc                  >  1:699668/1‑149 (MQ=255)
                                                                                                                              ggAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCGCa                 >  2:1216644/1‑149 (MQ=255)
                                                                                                                              ggAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCGCa                 <  4:1615877/149‑1 (MQ=255)
                                                                                                                              ggAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCGCa                 >  2:1614961/1‑149 (MQ=255)
                                                                                                                                   ccccGCGCCTCAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGGCGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCttt                                                                 <  2:904806/95‑1 (MQ=255)
                                                                                                                                   ccccGCGCCTCAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATgtcgt                                                                                >  3:1012892/2‑81 (MQ=255)
                                                                                                                                   ccccGCGCCTCAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATgtcgt                                                                                <  4:1012892/80‑1 (MQ=255)
                                                                                                                                   ccccGCGCCTCAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCGCATCAcc            >  3:563247/2‑149 (MQ=255)
                                                                                                                                   ccccGCGCCTCAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCGCATCAcc            >  2:194578/2‑149 (MQ=255)
                                                                                                                                    cccGCGCCTCAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCGCATCACCa           >  3:657227/1‑149 (MQ=255)
                                                                                                                                    cccGCGCCTCAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCGCATCACCa           >  2:73232/1‑149 (MQ=255)
                                                                                                                                     ccGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCGCATCACCaa          >  2:1394660/1‑149 (MQ=255)
                                                                                                                                     ccGCGCCTGAGGCGCGGGGGTCAGACCGGTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCGCATCACCaa          >  3:1449321/1‑149 (MQ=255)
                                                                                                                                         gcCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCGCATCACCAACGaa      <  1:503088/149‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                                    
GGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCGCATCACCAACGAATGGC  >  NC_000913/4082555‑4082844

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: