Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 765,135 T→C 5.3% intergenic (+86/+18) sucD → / ← mngR succinyl‑CoA synthetase, NAD(P)‑binding, alpha subunit/transcriptional repressor for the mannosyl‑D‑glycerate catabolic operon

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913765,1350TC 5.3% 559.9 / 11.6 166intergenic (+86/+18)sucD/mngRsuccinyl‑CoA synthetase, NAD(P)‑binding, alpha subunit/transcriptional repressor for the mannosyl‑D‑glycerate catabolic operon
Reads supporting (aligned to +/- strand):  ref base T (80/77);  new base C (4/5);  total (84/82)
Fisher's exact test for biased strand distribution p-value = 7.45e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 172 total aligned reads displayed.

GGCGTGAAAACCGTTCGCAGCCTGGCGGATATCGGTGAAGCACTGAAAACTGTTCTGAAATAAATATCTGTAATAAGAAATAGCCCTCGCCGCTTCCCTCTACAGGAATGGCGAAGGGCTGTCGGTTTCGACATGGTTGGCCATCGTATGATGGCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATCGGTATTAAAAGCGTTGCGGCTATATTCAAACACCCGACCATCAACTAAATATCCACGCGATACTTTTTCAAGAATCGGCTT  >  NC_000913/764987‑765281
                                                                                                                                                    |                                                                                                                                                  
ggCGTGAAAACCGTTCGCAGCCTGGCGGATATCGGTGAATCACTGAAAACTGTTCTGAAATAAATATCTGTAATAAGAAATAGCCCTCGTCGCTTCCCTCTACAGGAATGGCGAAGGGCTGTCGGTTTCGACATGGTTGGCCATCGTat                                                                                                                                                    <  4:1052764/149‑1 (MQ=255)
      aaaaCCGTTCGCAGCCTGGCGGATATCGGTGAAGCACTGAAAACTGTTCTGAAATAAATATCTGTAATAAGAAATAGCCCTCGCCGCTTCCCTCTACAGGAATGGCGAAGGGCTGTCGGTTTCGACATGGTTGGCCATCGTATGATGGc                                                                                                                                              >  4:911997/1‑149 (MQ=255)
        aaCCGTTCGCAGCCTGGCGGATATCGGTGAAGCACTGAAAACTGTTCTGAAATAAATATCTGTAATAAGAAATAGCCCTCGCCGCTTCCCTCTACAGGAATGGCGAAGGGCTGTCGGTTTCGACATGGTTGGCCATCGTATGATGGCCt                                                                                                                                            >  4:859818/1‑149 (MQ=255)
         aCCGTTCGCAGCCTGGCGGATATCGGTGAAGCACTGAAAACTGTTCTGAAATAAATATCTGTAATAAGAAATAGCCCTCGCCGCTTCCCTCTACAGGAATGGCGAAGGGCTGTCGGTTTCGACATGGTTGGCCATCATACGATGGccaa                                                                                                                                           <  1:445600/149‑3 (MQ=255)
           cGTTCGCAGCCTGGCGGATATCGGTGAAGCACTGAAAACTGTTCTGAAATAAATATCTGTAATAAGAAATAGCCCTCGCCGCTTCCCTCTACAGGAATGGCGAAGGGCTGTCGGTTTCGACATGGTTGGCCATCGTATGATGGCCtttt                                                                                                                                         >  4:1197827/1‑149 (MQ=255)
           cGTTCGCAGCCTGGCGGATATCGGTGAAGCACTGAAAACTGTTCTGAAATAAATATCTGTAATAAGAAATAGCCCTCGCCGCTTCCCTCTACAGGAATGGCGAAGGGCTGTCGGTTTCGACATGGTTGGCCATCGTATGATGGCCtttt                                                                                                                                         >  3:1625422/1‑149 (MQ=255)
            gTTCGCAGCCTGGCGGATATCGGTGAAGCACTGAAAACTGTTCTGAAATAAATATCTGTAATAAGAAATAGCCCTCGCCGCTTCCCTCTACAGGAATGGCGAAGGGCTGTCGGTTTCGACATGGTTGGCCATCGTATGATGGCCttttt                                                                                                                                        <  1:1001990/149‑1 (MQ=255)
             ttCGCAGCCTGGCGGATATCGGTGAAGCACTGAAAACTGTTCTGAAATAAATATCTGTAATAAGAAATAGCCCTCGCCGCTTCCCTCTACAGGAATGGCGAAGGGCTGTCGGTTTCGACATGGTTGGCCATCGTATGATGGCCtttttt                                                                                                                                       <  4:234400/149‑1 (MQ=255)
               cGCAGCCTGGCGGATATCGGTGAAGCACTGAAAACTGTTCTGAAATAAATATCTGTAATAAGAAATAGCCCTCGCCGCTTCCCTCTACAGGAATGGCGAAGGGCTGTCGGTTTCGACATGGTTGGCCATCGTATGATGGCCTTTTTtgt                                                                                                                                     <  3:770462/149‑1 (MQ=255)
                gCAGCCTGGCGGATATCGGTGAAGCACTGAAAACTGTTCTGAAATAAATATCTGTAATAAGAAATAGCCCTCGCCGCTTCCCTCTACAGGAATGGCGAAGGGCTGTCGGTTTCGACATGGTTGGCCATCGTATGATGGCCTTTTTtgtg                                                                                                                                    >  1:523194/1‑149 (MQ=255)
                 cAGCCTGGCGGATATCGGTGAAGCACTGAAAACTGTTCTGAAATAAATATCTGTAATAAGAAATAGCCCTCGCCGCTTCCCTCTACAGGAATGGCGAAGGGCTGTCGGTTTCGACATGGTTGGCCATCGTATGATGGCCTTTTTTGTGc                                                                                                                                   <  3:398147/149‑1 (MQ=255)
                   gCCTGGCGGATATCGGTGAAGCACTGAAAACTGTTCTGAAATAAATATCTGTAATAAGAAATAGCCCTCGCCGCTTCCCTCTACAGGAATGGCGAAGGGCTGTCGGTTTCGACATGGTTGGCCATCGTATGATGGCCTTTTTTGTGCtt                                                                                                                                 >  3:1213459/1‑149 (MQ=255)
                   gCCTGGCGGATATCGGTGAAGCACTGAAAACTGTTCTGAAATAAATATCTGTAATAAGAAATAGCCCTCGCCGCTTCCCTCTACAGGAATGGCGAAGGGCTGTCGGTTTCGACATGGTTGGCCATCGTATGATGGCCTTTTTTGTGCtt                                                                                                                                 >  3:1516494/1‑149 (MQ=255)
                   gCCCGGCGGATATCGGTGAAGCACTGAAAACTGTTCTGAAATAAATATCTGTAATAAGAAATAGCCCTCGCCGCTTCCCTCTACAGGAATGGCGAAGGGCTGTCGGTTTCGACATGGTTGGCCATCGTATGATGGCCTTTTTTGTGCtt                                                                                                                                 >  3:541575/1‑149 (MQ=255)
                     cTGGCGGATATCGGTGAAGCACTGAAAACTGTTCTGAAATAAATATCTATAATAAGAAATAGCCCTCGCCGCTTCCCTCTACAGGAATGGCGAAGGGCTGTCGGTTTCGACATGGTTGGCCATCGTATGATGGCCTTTTTTGTGCTTAt                                                                                                                               >  4:1306085/1‑149 (MQ=255)
                          ggATATCGGTGAAGCACTGAAAACTGTTCTGAAATAAATATCTGTAATAAGAAATAGCCCTCGCCGCTTCCCTCTACAGGAATGGCGAAGGGCTGTCGGTTTCGACATGGTTGGCCATCGTATGATGGCCTTTTTTGTGCTTATCGCga                                                                                                                          >  3:739172/1‑149 (MQ=255)
                           gATATCGGTGAAGCACTGAAAACTGTTCTGAAATAAATATCTGTAATAAGAAATAGCCCTCGCCGCTTCCCTCTACAGGAATGGCGAAGGGCTGTCGGTTTCGACATGGTTGGCCATCGTATGATGGCCTTTTTTGTGCTTATCGCgat                                                                                                                         <  4:185871/149‑1 (MQ=255)
                                       gCACTGAAAACTGTTCTGAAATAAATATCTGTAATAAGAAATAGCCCTCGCCGCTTCCCTCTACAGGAATGGCGAAGGGCTGTCGGTTTCGACATGGTTGGCCATCGTATGATGGCCTTTTTTGTGCTTATCGCGATGATTTTCGCTgc                                                                                                             >  1:321496/1‑149 (MQ=255)
                                          cTGAAAACTGTTCTGAAATAAATATCTGTAATAAGAAATAGCCCTCGCCGCTTCCCTCTACAGGAATGGCGAAGGGCTGTCGGTTTCGACATGGTTGGCCATCGTATGATGGCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCt                                                                                                          >  2:1568587/1‑149 (MQ=255)
                                          cTGAAAACTGTTCTGAAATAAATATCTGTAATAAGAAATAGCCCTCGCCGCTTCCCTCTACAGGAATGGCGAAGGGCTGTCGGTTTCGACATGGTTGGCCATCGTATGATGGCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCt                                                                                                          >  1:108337/1‑149 (MQ=255)
                                             aaaaCTGTTCTGAAATAAATATCTGTAATAAGAAATAGCCCTCGCCGCTTCCCTCTACAGGAATGGCGAAGGGCTGTCGGTTTCGACATGGTTGGCCATCGTATGATGGCCTTTTTTGTGCTTATc                                                                                                                              >  1:1255567/1‑126 (MQ=255)
                                             aaaaCTGTTCTGAAATAAATATCTGTAATAAGAAATAGCCCTCGCCGCTTCCCTCTACAGGAATGGCGAAGGGCTGTCGGTTTCGACATGGTTGGCCATCGTATGATGGCCTTTTTTGTGCTTATCGCga                                                                                                                          <  2:1426945/130‑1 (MQ=255)
                                             aaaaCTGTTCTGAAATAAATATCTGTAATAAGAAATAGCCCTCGCCGCTTCCCTCTACAGGAATGGCGAAGGGCTGTCGGTTTCGACATGGTTGGCCATCGTATGATGGCCTTTTTTGTGCTTATCGCga                                                                                                                          >  1:1426945/1‑130 (MQ=255)
                                             aaaaCTGTTCTGAAATAAATATCTGTAATAAGAAATAGCCCTCGCCGCTTCCCTCTACAGGAATGGCGAAGGGCTGTCGGTTTCGACATGGTTGGCCATCGTATGATGGCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATc                                                                                                       >  2:582352/1‑149 (MQ=255)
                                                 cTGTTCTGAAATAAATATCTGTAATAAGAAATAGCCCTCGCCGCTTCCCTCTACAGGAATGGCGAAGGGCTGTCGGTTTCGACATGGTTGGCCATCGTATGATGGCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAggg                                                                                                   <  2:524103/149‑1 (MQ=255)
                                                  tGTTCTGAAATAAATATCTGTAATAAGAAATAGCCCTCGCCGCTTCCCTCTACAGGAATGGCGAAGGGCTGTCGGTTTCGACATGGTTGGCCATCGTATGATGGCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGt                                                                                                  >  3:1227695/1‑149 (MQ=255)
                                                     tCTGAAATAAATATCTGTAATAAGAAATAGCCCTCGCCGCTTCCCTCTACAGGAATGGCGAAGGGCTGTCGGTTTCGACATGGTTGGCCATCGTATGATGGCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTaaa                                                                                               >  1:215582/1‑149 (MQ=255)
                                                      cTGAAATAAATCTCTGTAATAAGAAATAGCCCTCGCCGCTTCCCTCTACAGGAATGGCGAAGGGCTGTCGGTTTCGACATGGTTGGCCATCGTatgat                                                                                                                                                 <  1:1120393/98‑1 (MQ=255)
                                                      cTGAAATAAATATCTGTAATAAGAAATAGCCCTCGCCGCTTCCCTCTACAGGAATGGCGAAGGGCTGTCGGTTTCGACATGGTTGGCCATCGTatgat                                                                                                                                                 >  2:1120393/1‑98 (MQ=255)
                                                      cTGAAATAAATATCTGTAATAAGAAATAGCCCTCGCCGCTTCCCTCTACAGGAATGGCGAAGGGCTGTCGGTTTCGACATGGTTGGCCATCGTATGATGGCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAAt                                                                                              <  1:1654488/149‑1 (MQ=255)
                                                          aataaataTCTGTAATAAGAAATAGCCCTCGCCGCTTCCCTCTACAGGAATGGCGAAGGGCTGTCGGTTTCGACATGGTTGGCCATCGTATGATGGCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTtat                                                                                          <  2:1458015/149‑1 (MQ=255)
                                                            taaataTCTGTAATAAGAAATAGCCCTCGCCGCTTCCCTCTACAGGAATGGCGAAGGGCTGTCGGTTTCGACATGGTTGGCCATCGTATGATGGCCTTTTTtgt                                                                                                                                     <  3:1450922/104‑1 (MQ=255)
                                                               atatCTGTAATAAGAAATAGCCCTCGCCGCTTCCCTCTACAGGAATGGCGAAGGGCTGTCGGTTTCGACATGGTTGGCCATCGTATGATGGCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCa                                                                                     <  1:1168391/149‑1 (MQ=255)
                                                                tatCTGTAATAAGAAATAGCCCTCGCCGCTTCCCTCTACAGGAATGGCGAAGGGCTGTCGGTTTCGACATGGTTGGCCATCGTATGATGGCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCAt                                                                                    <  4:597799/149‑1 (MQ=255)
                                                                 atCTGTAATAAGAAATGGCCCTCGCCGCTTCCCTCTACAGGAATGGCGAAGGGCTGTCGGTTTCGACATGGTTGGCCATCGTATGATGGCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATc                                                                                   <  1:607740/149‑1 (MQ=255)
                                                                 atCTGTAATAAGAAATAGCCCTCGCCGCTTCCCTCTACAGGAATGGCGAAGGGCTGTCGGTTTCGACATGGTTGGCCATCGTATGATGGCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATc                                                                                   <  2:704761/149‑1 (MQ=255)
                                                                  tCTGTAATAAGAAATAGCCCTCGCCGCTTCCCTCTACAGGAATGGCGAAGGGCTGTCGGTTTCGACATGGTTGGCCATCGTATGATGGCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAgg                                                                                                    >  1:143112/1‑131 (MQ=255)
                                                                  tCTGTAATAAGAAATAGCCCTCGCCGCTTCCCTCTACAGGAATGGCGAAGGGCTGTCGGTTTCGACATGGTTGGCCATCGTATGATGGCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATCg                                                                                  >  3:207101/1‑149 (MQ=255)
                                                                  tCTGTAATAAGAAATAGCCCTCGCCGCTTCCCTCTACAGGAATGGCGAAGGGCTGTCGGTTTCGACATGGTTGGCCATCGTATGATGGCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATCg                                                                                  <  2:1195207/149‑1 (MQ=255)
                                                                    tGTAATAAGAAATAGCCCTCGCCGCTTCCCTCTACAGGAATGGCGAAGGGCTGTCGGTTTCGACATGGTTGGCCATCGTATGATGGCCTTTTTtgt                                                                                                                                     >  2:1321046/1‑96 (MQ=255)
                                                                    tGTAATAAGAAATAGCCCTCGCCGCTTCCCTCTACAGGAATGGCGAAGGGCTGTCGGTTTCGACATGGTTGGCCATCGTATGATGGCCTTTTTtgt                                                                                                                                     <  1:1321046/96‑1 (MQ=255)
                                                                    tGTAATAAGAAATAGCCCTCGCCGCTTCCCTCTACAGGAATGGCGAAGGGCTGTCGGTTTCGACATGGTTGGCCATCGTATGATGGCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATCGGt                                                                                >  2:365837/1‑149 (MQ=255)
                                                                     gTAATAAGAAATAGCCCTCGCCGCTTCCCTCTACAGGAATGGCGAAGGGCTGTCGGTTTCGACATGGTTGGCCATCGTATGATGGCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATCGGTa                                                                               <  4:1227695/149‑1 (MQ=255)
                                                                     gTAATAAGAAATAGCCCTCGCCGCTTCCCTCTACAGGAATGGCGAAGGGCTGTCGGTTTCGACATGGTTGGCCATCGTATGATGGCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATCGGTa                                                                               <  1:1582796/149‑1 (MQ=255)
                                                                     gTAATAAGAAATAGCCCTCGCCGCTTCCCTCTACAGGAATGGCGAAGGGCTGTCGGTTTCGACATGGTTGGCCATCGTATGATGGCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATCGGTa                                                                               <  2:691196/149‑1 (MQ=255)
                                                                      taataaGAAATAGCCCTCGCCGCTTCCCTCTACAGGAATGGCGAAGGGCTGTCGGTTTCGACATGGTTGGCCATCGTATGATGGCCTTTTTTGTGCTTATCGCGATGATTTTCGCt                                                                                                               <  3:1339045/116‑1 (MQ=255)
                                                                      taataaGAAATAGCCCTCGCCGCTTCCCTCTACAGGAATGGCGAAGGGCTGTCGGTTTCGACATGGTTGGCCATCGTATGATGGCCTTTTTTGTGCTTATCGCGATGATTTTCGCt                                                                                                               >  4:1339045/1‑116 (MQ=255)
                                                                       aataaGAAATAGCCCTCGCCGCTTCCCTCTACAGGAATGGCGAAGGGCTGTCGGTTTCGACATGGTTGGCCATCGTATGATGGCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATCGGTAtt                                                                             <  1:101297/149‑1 (MQ=255)
                                                                         taaGAAATAGCCCTCGCCGCTTCCCTCTACAGGAATGGCGAAGGGCTGTCGGTTTCGACATGGTTGGCCATCGTATGATGGCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATCGGTATTaa                                                                           >  3:83971/1‑149 (MQ=255)
                                                                           aGAAATAGCCCTCGCCGCTTCCCTCTACAGGAATGGCGAAGGGCTGTCGGTTTCGACATGGTTGGCCATCGTATGATGGCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATCGGTATTaaaa                                                                         >  2:1452978/1‑149 (MQ=255)
                                                                             aaaTAGCCCTCGCCGCTTCCCTCTACAGGAATGGCGAAGGGCTGTCGGTTTCGACATGGTTGGCCATCGTATGATGGCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATCGGTa                                                                               <  4:1567387/141‑1 (MQ=255)
                                                                             aaaTAGCCCTCGCCGCTTCCCTCTACAGGAATGGCGAAGGGCTGTCGGTTTCGACATGGTTGGCCATCGTATGATGGCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATCGGTa                                                                               >  3:1567387/1‑141 (MQ=255)
                                                                                     cTCGCCGCTTCCCTCTACAGGAATGGCGAAGGGCTGTCGGTTTCGACATGGTTGGCCATCGTATGATGGCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATCGGTATTAAAAGCGTTGCGGc                                                               <  1:1130279/149‑1 (MQ=255)
                                                                                      tCGCCGCTTCCCTCTACAGGAATGGCGAAGGGCTGTCGGTTTCGACATGGTTGGCCATCGTATGATGGCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATCGGTATTAAAAGCGTTGCGGCt                                                              >  3:1350382/1‑149 (MQ=255)
                                                                                      tCGCCGCTTCCCTCTACAGGAATGGCGAAGGGCTGTCGGTTTCGACATGGTTAGCCATCGTATGATGGCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATCGGTATTAAAAGCGTTGCGGCt                                                              <  4:1471449/149‑1 (MQ=255)
                                                                                       cgccgcTTCCCTCTACAGGAATGGCGAAGGGCTGTCGGTTTCGACATGGTTGGCCATCGTATGATGGCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCTTCGGTATTAAAAGCGTTGCGGCta                                                             >  3:1080326/1‑149 (MQ=255)
                                                                                               cccTCTACAGGAATGGCGAAGGGCTGTCGGTTTCGACATGGTTGGCCATCGTATGATGGCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATCGGTATTAAAAGCGTTGCGGCTATATTCaaa                                                     <  3:889615/149‑1 (MQ=255)
                                                                                                  tctACAGGAATGGCGAAGGGCTGTCGGTTTCGACATGGTTGGCCATCGTATGATGGCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATCGGTATTAAAAGCGTTGCGGCTATATTCAAacac                                                  >  1:1220693/1‑149 (MQ=255)
                                                                                                   ctACAGGAATGGCGAAGGGCTGTCGGTTTCGACATGGTTGGCCATCGTATGATGGCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATCGGTATTAAAAGCGTTGCGGCTATATTCAAACAcc                                                 <  3:1306085/149‑1 (MQ=255)
                                                                                                    tACAGGAATGGCGAAGGGCTGTCGGTTTCGACATGGTTGGCCATCGTATGATGGCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATCGGTATTAAAAGCGTTGCGGCTATATTCAAACAccc                                                >  3:1405535/1‑149 (MQ=255)
                                                                                                    tACAGGAATGGCGAAGGGCTGTCGGTTTCGACATGGTTGGCCATCGTATGATGGCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATCGGTATTAAAAGCGTTGCGGCTATATTCAAACAccc                                                <  1:258127/149‑1 (MQ=255)
                                                                                                      cAGGAATGGCGAAGGGCTGTCGGTTTCGACATGGTTGGCCATCGTATGATGGCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATCGGTATTAAAAGCGTTGCGGCTATATTCAAACACCCGa                                              <  4:1433870/149‑1 (MQ=255)
                                                                                                       aGGAATGGCGAAGGGCTGTCGGTTTCGACATGGTTGGCCATCGTATGATGGCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTaaa                                                                                               <  4:650191/99‑1 (MQ=255)
                                                                                                       aGGAATGGCGAAGGGCTGTCGGTTTCGACATGGTTGGCCATCGTATGATGGCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTaaa                                                                                               >  3:650191/1‑99 (MQ=255)
                                                                                                         gAATGGCGAAGGGCTGTCGGTTTCGACATGGTTGGCCATCGTATGATGGCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATCGGTATTAAAAGCGTTGCGGCTATATTCAAACACCCGACCa                                           >  4:1112809/1‑149 (MQ=255)
                                                                                                           aTGGCGAAGGGCTGTCGGTTTCGACATGGTTGGCCATCGTATGATGGCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATCGGTATTAAAAGCGTTGCGGCTATATTCAAACACCCGACCATc                                         <  3:911997/149‑1 (MQ=255)
                                                                                                              gCGAAGGGCTGTCGGTTTCGACATGGTTGGCCATCGTATGATGGCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATCGGTATTAAAAGCGTTGCGGCTATATTCAAACACCCGACCATCAAc                                      <  2:321496/149‑1 (MQ=255)
                                                                                                              gCGAAGGGCTGTCGGTTTCGACATGGTTGGCCATCGTATGATGGCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATCGGTATTAAAAGCGTTGCGGCTATATTCAAACACCCGACCATCAAc                                      <  3:558850/149‑1 (MQ=255)
                                                                                                              gCGAAGGGCTGTCGGTTTCGACATGGTTGGCCATCGTATGATGGCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATCGGTATTAAAAGCGTTGCGGCTATATTCAAACACCCGACCATCAAc                                      <  2:175558/149‑1 (MQ=255)
                                                                                                                  aGGGCTGTCGGTTTCGACATGGTTGGCCATCGTATGATGGCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATCGGTATTAAAAGCGTTGCGGCTATATTCAAACACCCGACCATCAACTaaa                                  <  2:100523/149‑1 (MQ=255)
                                                                                                                  aGGGCTGTCGGTTTCGACATGGTTGGCCATCGTATGATGGCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATCGGTATTAAAAGCGTTGCGGCTATATTCAAACACCCGACCATCAACTaaa                                  <  2:1299855/149‑1 (MQ=255)
                                                                                                                     gCTGTCGGTTTCGACATGGTTGGCCATCGTATGATGGCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATCGGTATTAAAAGCGTTGCGGCTATATTCAAACACCCGACCATCAACTAAatat                               <  4:541575/149‑1 (MQ=255)
                                                                                                                      cTGTCGGTTTCGACATGGTTGGCCATCGTATGATGGCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATCGGTATTAAAAGCGTTGCGGCTATATTCAAACACCCGACCATCAACTAAATATc                              <  3:461614/149‑1 (MQ=255)
                                                                                                                      cTGTCGGTTTCGACATGGTTGGCCATCGTATGATGGCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATCGGTATTAAAAGCGTTGCGGCTATATTCAAACACCCGACCATCAACTAAATATc                              >  3:1195759/1‑149 (MQ=255)
                                                                                                                        gTCGGTTTCGACATGGTTGGCCATCGTATGATGGCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATCGGTATTAAAAGCGTTGCGGCTATATTCAAACACCCGACCATCAACTAAATATCCa                            <  4:83971/149‑1 (MQ=255)
                                                                                                                         tCGGTTTCGACATGGTTGGCCATCGTATGATGGCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATCGGTATTAAAAGCGTTGCGGCTATATTCAAACACCCGACCATCAACTAAATATCCAc                           <  4:207101/149‑1 (MQ=255)
                                                                                                                         tCGGTTTCGACATGGTTGGCCATCGTATGATGGCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATCGGTATTAAAAGCGTTGCGGCTATATTCAAACACCCGACCATCAACTAAATATCCAc                           <  1:536427/149‑1 (MQ=255)
                                                                                                                          cGGTTTCGACATGGTTGGCCATCGTATGATGGCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATCGGTATTAAAAGCGTTGCGGCTATATTCAAACACCCGACCATCAACTAAATATCCAcg                          <  2:639146/149‑1 (MQ=255)
                                                                                                                               tCGACATGGTTGGCCATCGTATGATGGCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATCGGTATTAAAAGCGTTGCGGCTATATTCAAACACCCGACCATCAACTAAATATCCACGCGATa                     <  2:244381/149‑1 (MQ=255)
                                                                                                                                  aCATGGTTGGCCATCGTATGATGGCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATCGGTATTAAAAGCGTTGCGGCTATATTCAAACACCCGACCATCAACTAAATATCCACGCGATACtt                  >  1:1556872/1‑149 (MQ=255)
                                                                                                                                    aTGGTTGGCCATCGTATGATGGCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATCGGTATTAAAAGCGTTGCGGCTATATTCAAACACCCGACCATCAACTAAATATCCACGCGATACttta                >  4:1451231/1‑148 (MQ=255)
                                                                                                                                     tGGTTGGCCATCGTATGATGGCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATCGGTATTAAAAGCGTTGCGGCTATATTCAAACACCCGACCATCAACTAAATATCCACGCGATACttttt               <  3:813041/149‑1 (MQ=255)
                                                                                                                                     tGGTTGGCCATCGTATGATGGCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATCGGTATTAAAAGCGTTGCGGCTATATTCAAACACCCGACCATCAACTAAATATCCACGCGATACttttt               >  4:835183/1‑149 (MQ=255)
                                                                                                                                        ttGGCCATCGTATGATGGCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATCGGTATTAAAAGCGTTGCGGCTATATTCAAACACCCGACCATCAACTAAATATCCACGCGATACTTTTTCaa            <  3:1274180/149‑1 (MQ=255)
                                                                                                                                        ttGGCCATCGTATGATGGCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATCGGTATTAAAAGCGTTGCGGCTATATTCAAACACCCGACCATCAACTAAATATCCACGCGATACTTTTTCaa            <  2:1105638/149‑1 (MQ=255)
                                                                                                                                         aggCCATCATACGATGGCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATCGGTATTAAAAGCGTTGCGGCTATATTCAAACACCCGACCATCAACTAAATATCCACGCGATACTTTTTCAAg           >  2:1275148/2‑149 (MQ=255)
                                                                                                                                         aggCCATCATACGATGGCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATCGGTATTAAAAGCGTTGCGGCTATATTCAAACACCCGACCATCAACTAAATATCCACGCGATACTTTTTCAAg           >  1:1273416/2‑149 (MQ=255)
                                                                                                                                          ggCCATCATACGATGGCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATCGGTATTAAAAGCGTTGCGGCTATATTCAAACACCCGACCATCAACTAAATATCCACGCGATACTTTTTCAAGa          >  2:1712974/1‑149 (MQ=255)
                                                                                                                                            ccATCGTATGATGGCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATCGGTATTAAAAGCGTTGCGGCTATATTCAAACACCCGACCATCAACTAAATATCCACGCGATACTTTTTCAAGAAt        >  1:663139/1‑149 (MQ=255)
                                                                                                                                             cATCGTATGATGGCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATCGGTATTAAAAGCGTTGCGGCTATATTCAAACACCCGACCATCAACTAAATATCCACGCGATACTTTTTCAAGAATc       >  2:904847/1‑149 (MQ=255)
                                                                                                                                             cATCGTACGATGGCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATCGGTATTAAAAGCGTTGCGGCTATATTCAAACACCCGACCATCAACTAAATATCCACGCGATACTTTTTCAAGAATc       <  3:1451231/149‑1 (MQ=255)
                                                                                                                                              aTCGTATGATGGCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATCGGTATTAAAAGCGTTGCGGCTATATTCAAACACCCGACCATCAACTAAATATCCACGCGATACTTTTTCAAGAATCg      >  4:700528/1‑149 (MQ=255)
                                                                                                                                              aTCGTATGATGGCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATCGGTATTAAAAGCGTTGCGGCTATATTCAAACACCCGACCATCAACTAAATATCCACGCGATACTTTTTCAAGAATCg      <  4:1516494/149‑1 (MQ=255)
                                                                                                                                               tCGTATGATGGCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATCGGTATTAAAAGCGTTGCGGCTATATTCAAACACCCGACCATCAACTAAATATCCACGCGATACTTTTTCAAGAATCgg     <  1:1452978/149‑1 (MQ=255)
                                                                                                                                                 gTATGATGGCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATCGGTa                                                                               >  3:1179881/1‑73 (MQ=255)
                                                                                                                                                 gTATGATGGCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATCGGTa                                                                               <  4:1179881/73‑1 (MQ=255)
                                                                                                                                                 gTATGATGGCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATCGGTATTAAAAGCGTTGCGGCTATATTCAAACACCCGACCATCAACTAAATATCCACGCGATACTTTTTCAAGAATCGGCt   >  1:376715/1‑149 (MQ=255)
                                                                                                                                                  tATGATGGCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATCGGTATTAAAAGCGTTGCGGCTATATTCAAACACCCGACCATCAACTAAATCTCCACGCGATACTTTTTCAAGAATCGGCtt  >  1:1574838/1‑149 (MQ=255)
                                                                                                                                                    tgatgGCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATCGGTATTAAAAGCGTTGCGGCTATATTCAAACACCCGACCATCAACTAAATATCCACGCGATACtttt                >  1:883425/1‑133 (MQ=255)
                                                                                                                                                    tgatgGCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATCGGTATTAAAAGCGTTGCGGCTATATTCAAACACCCGACCATCAACTAAATATCCACGCGATACtttt                <  2:883425/133‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
GGCGTGAAAACCGTTCGCAGCCTGGCGGATATCGGTGAAGCACTGAAAACTGTTCTGAAATAAATATCTGTAATAAGAAATAGCCCTCGCCGCTTCCCTCTACAGGAATGGCGAAGGGCTGTCGGTTTCGACATGGTTGGCCATCGTATGATGGCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATCGGTATTAAAAGCGTTGCGGCTATATTCAAACACCCGACCATCAACTAAATATCCACGCGATACTTTTTCAAGAATCGGCTT  >  NC_000913/764987‑765281

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: