Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 773,145 T→C 6.7% L35P (CTC→CCC)  cydB → cytochrome d terminal oxidase, subunit II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913773,1450TC 6.7% 490.9 / 16.2 151L35P (CTC→CCC) cydBcytochrome d terminal oxidase, subunit II
Reads supporting (aligned to +/- strand):  ref base T (67/73);  new base C (3/7);  total (71/80)
Fisher's exact test for biased strand distribution p-value = 3.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01

Only 100 of 152 total aligned reads displayed.

TTCCACGACTACTCAGCCGGCACGCTAAGACAGGAGTCGTCAAATGATCGATTATGAAGTATTGCGTTTTATCTGGTGGCTGCTGGTTGGCGTTCTGCTGATTGGTTTTGCAGTCACTGACGGTTTCGACATGGGGGTGGGCATGCTCACCCGTTTCCTCGGTCGTAACGACACCGAGCGTCGAATTATGATTAACTCCATTGCACCACACTGGGACGGTAACCAGGTTTGGCTGATCACCGCGGGCGGCGCACTCTTTGCTGCCTGGCCGATGGTCTATGCCGCTGCGTTCTCC  >  NC_000913/772999‑773293
                                                                                                                                                  |                                                                                                                                                    
ttCCACGACTACTCAGCCGGCACGCTAAGACAGGAGTCGTCAAATGATCGATTATGAAGTATTGCGTTTTATCTGGTGGCTGCTGGTTGGCGTTCTGTTGATTGGTTTTGCAGTCACTGACGGTTTCGACATGGGGGTGAGCATGCCCa                                                                                                                                                    >  2:1039345/1‑149 (MQ=255)
ttCCACGACTACTCAGCCGGCACGCTAAGACAGGAGTCGTCAAATGATCGATTATGAAGTATTGCGTTTTATCTGGTGGCTGCTGGTTGGCGTTCTGTTGATTGGTTTTGCAGTCACTGACGGTTTCGACATGGGGGTGAGCATGCCCa                                                                                                                                                    >  4:688258/1‑149 (MQ=255)
 tCCACGACTACTCAGCCGGCACGCTAAGACAGGAGTCGTCAAATGATCGATTATGAAGTATTGCGTTTTATCTGGTGGCTGCTGGTTGGCGTTCTGCTGATTGGTTTTGCAGTCACTGACGGTTTCGACATGGGGGTGGGCATGCTCAc                                                                                                                                                   <  3:442511/149‑1 (MQ=255)
   cACGACTACTCAGCCGGCACGCTAAGACAGGAGTCGTCAAATGATCGATTATGAAGTATTGCGTTTTATCTGGTGGCTGCTGGTTGGCGTTCTGCTGATTGGTTTTGCAGTCACTGACGGTTTCGACATGGGGGTGGGCATGCTCAccc                                                                                                                                                 <  3:580869/149‑1 (MQ=255)
   cACGACTACTCAGCCGGCACGCTAAGACAGGAGTCGTCAAATGATCGATTATGAAGTATTGCGTTTTATCTGGTGGCTGCTGGTTGGCGTTCTGCTGATTGGTTTTGCAGTCACTGACGGTTTCGACATGGGGGTGGGCATGCTCAccc                                                                                                                                                 <  3:135540/149‑1 (MQ=255)
   cACGACTACTCAGCCGGCACGCTAAGACAGGAGTCGTCAAATGATCGATTATGAAGTATTGCGTTTTATCTGGTGGCTGCTGGTTGGCGTTCTGCTGATTGGTTTTGCAGTCACTGACGGTTTCGACATGGGGGTGGGCATGCTCAccc                                                                                                                                                 <  1:1403984/149‑1 (MQ=255)
      gACTACTCAGCCGGCACGCTAAGACAGGAGTCGTCAAATGATCGATTATGAAGTATTGCGTTTTATCTGGTGGCTGCTGGTTGGCGTTCTGCTGATTGGTTTTGCAGTCACTGACGGTTTCGACATGGGGGTGGGCATGCTCACCCGtt                                                                                                                                              <  2:1336822/149‑1 (MQ=255)
      gACTACTCAGCCGGCACGCTAAGACAGGAGTCGTCAAATGATCGATTATGAAGTATTGCGTTTTATCTGGTGGCTGCTGGTTGGCGTTCTGCTGATTGGGTTTGCAGTCACTGACGGTTTCGACATGGGGGTGGGCATGCTCACCCGtt                                                                                                                                              >  1:836661/1‑149 (MQ=255)
       actactCAGCCGGCACGCTAAGACAGGAGTCGTCAAATGATCGATTATGAAGTATTGCGTTTTATCTGGTGGCTGCTGGTTGGCGTTCTGCTGATTGGTTTTGCAGTCACTGACGGTTTCGACATGGGGGTGAGCATGCCCAcccccat                                                                                                                                             <  4:964249/149‑5 (MQ=255)
       actactCAGCCGGCACGCTAAGACAGGAGTCGTCAAATGATCGATTATGAAGTATTGCGTTTTATCTGGTGGCTGCTGGTTGGCGTTCTGCTGATTGGTTTTGCAGTCACTGACGGTTTCGACATGGGGGTGAGCATGCCCAcccccat                                                                                                                                             <  2:361208/149‑5 (MQ=255)
         tactCAGCCGGCACGCTAAGACAGGAGTCGTCAAATGATCGATTATGAAGTATTGCGTTTTATCTGGTGGCTGCTGGTTGGCGTTCTGCTGATTGGTTTTGCAGTCACTGACGGTTTCGACATGGGGGTGAGCATGCCCAcccccatgt                                                                                                                                           <  4:898546/149‑7 (MQ=255)
          actCAGCCGGCACGCTAAGACAGGAGTCGTCAAATGATCGATTATGAAGTATTGCGTTTTATCTGGTGGCTGCTGGTTGGCGTTCTGTTGATTGGTTTTGCAGTCACTGACGGTTTCGACATGGGGGTGAGCATGCCCAcccccatgtc                                                                                                                                          <  1:1039345/149‑8 (MQ=255)
          actCAGCCGGCACGCTAAGACAGGAGTCGTCAAATGATCGATTATGAAGTATTGCGTTTTATCTGGTGGCTGCTGGTTGGCGTTCTGCTGATTGGTTTTGCAGTCACTGACGGTTTCGACATGGGGGTGAGCATGCTCACCCGTTTCCt                                                                                                                                          >  2:1491200/1‑149 (MQ=255)
            tCAGCCGGCACGCTAAGACAGGAGTCGTCAAATGATCGATTATGAAGTATTGCGTTTTATCTGGTGGCTGCTGGTTGGCGTTCTGCTGATTGGTTTTGCAGTCACTGACGGTTTCGACATGGGGGTGGGCATGCTCACCCGTTTCCTCg                                                                                                                                        >  3:1425922/1‑149 (MQ=255)
            tCAGCCGGCACGCTAAGACAGGAGTCGTCAAATGATCGATTATGAAGTATTGCGTTTTATCTGGTGGCTGCTGGTTGGCGTTCTGCTGATTGGTTTTGCAGTCACTGACGGTTTCGACATGGGGGTGGGCATGCTCACCCGTTTCCTCg                                                                                                                                        >  2:1137612/1‑149 (MQ=255)
               gCCGGCACGCTAAGACAGGAGTCGTCAAATGATCGATTATGAAGTATTGCGTTTTATCTGGTGGCTGCTGGTTGGCGTTCTGCTGATTGGTTTTGCAGTCACTGACGGTTTCGACATGGGGGTGGGCATGCTCACCCGTTTCCTCGGTc                                                                                                                                     <  3:500237/149‑1 (MQ=255)
               gCCGGCACGCTAAGACAGGAGTCGTCAAATGATCGATTATGAAGTATTGCGTTTTATCTGGTGGCTGCTGGTTGGCGTTCTGCTGATTGGTTTTGCAGTCACTGACGGTTTCGACATGGGGGTGGGCATGCTCACCCGTTTCCTCGGTc                                                                                                                                     <  2:1378296/149‑1 (MQ=255)
               gCCGGCACGCTAAGACAGGAGTCGTCAAATGATCGATTATGAAGTATTGCGTTTTATCTGGTGGCTGCTGGTTGGCGTTCTGCTGATTGGTTTTGCAGTCACTGACGGTTTCGACATGGGGGTGGGCATGCTCACCCGTTTCCTCGGTc                                                                                                                                     <  1:1276505/149‑1 (MQ=255)
                   gCACGCTAAGACAGGAGTCGTCAAATGATCGATTATGAAGTATTGCGTTTTATCTGGTGGCTGCTGGTTGGCGTTCTGCTGATTGGTTTTGCAGTCACTGACGGTTTCGACATGGGGGTGGGCATGCTCACCCGTTTCCTCGGTCGTaa                                                                                                                                 <  2:163056/149‑1 (MQ=255)
                       gCTAAGACAGGAGTCGTCAAATGATCGATTATGAAGTATTGCGTTTTATCTGGTGGCTGCTGGTTGGCGTTCTGCTGATTGGTTTTGCAGTCACTGACGGTTTCGACATGGGGGTGGGCATGCTCACCCGTTTCCTCGGTCGTAACGac                                                                                                                             >  4:1604125/1‑149 (MQ=255)
                         tAAGACAGGAGTCGTCAAATGATCGATTATGAAGTATTGCGTTTTATCTGGTGGCTGCTGGTTGGCGTTCTGCTGATTGGTTTTGCAGTCACTGACGGTTTCGACATGGGGGTGGGCATGCTCACCCGTTTcc                                                                                                                                           >  3:742394/1‑133 (MQ=255)
                         tAAGACAGGAGTCGTCAAATGATCGATTATGAAGTATTGCGTTTTATCTGGTGGCTGCTGGTTGGCGTTCTGCTGATTGGTTTTGCAGTCACTGACGGTTTCGACATGGGGGTGGGCATGCTCACCCGTTTcc                                                                                                                                           <  4:742394/133‑1 (MQ=255)
                         tAAGACAGGAGTCGTCAAATGATCGATTATGAAGTATTGCGTTTTATCTGGTGGCTGCTGGTTGGCGTTCTGCTGATTGGTTTTGCAGTCACTGACGGTTTCGACATGGGGGTGGGCATGCTCACCCGTTTCCTCGGTCGTAACGacac                                                                                                                           >  3:967810/1‑149 (MQ=255)
                          aaGACAGGAGTCGTCAAATGATCGATTATGAAGTATTGCGTTTTATCTGGTGGCTGCTGGTTGGCGTTCTGCTGATTGGTTTTGCAGTCACTGACGGTTTCGACATGGGGGTGGGCATGCTCACCCGTTTCCTCGGTCGTAACGACAcc                                                                                                                          >  2:1362054/1‑149 (MQ=255)
                           aGACAGGAGTCGTCAAATGATCGATTATGAAGTATTGCGTTTTATCTGGTGGCTGCTGGTTGGCGTTCTGCTGATTGGTTTTGCAGTCACTGACGGTTTCGACATGGGGGTGGGCATGCTCAccc                                                                                                                                                 <  3:1272683/125‑1 (MQ=255)
                           aGACAGGAGTCGTCAAATGATCGATTATGAAGTATTGCGTTTTATCTGGTGGCTGCTGGTTGGCGTTCTGCTGATTGGTTTTGCAGTCACTGACGGTTTCGACATGGGGGTGGGCATGCTCAccc                                                                                                                                                 >  4:1272683/1‑125 (MQ=255)
                            gACAGGAGTCGTCAAATGATCGATTATGAAGTATTGCGTTTTATCTGGTGGCTGCTGGTTGGCGTTCTGCTGATTGGTTTTGCAGTCACTGACGGTTTCGACATGGGGGTGGGCATGCTCACCCGTTTCCTCGGTCGTAACGACACCGa                                                                                                                        <  3:1604125/149‑1 (MQ=255)
                             aCAGGAGTCGTCAAATGATCGATTATGAAGTATTGCGTTTTATCTGGTGGCTGCTGGTTGGCGTTCTGCTGATTGGTTTTGCAGTCACTGACGGTTTCGACATGGGGGTGGGCATGCTCAcc                                                                                                                                                  >  2:1693875/1‑122 (MQ=255)
                             aCAGGAGTCGTCAAATGATCGATTATGAAGTATTGCGTTTTATCTGGTGGCTGCTGGTTGGCGTTCTGCTGATTGGTTTTGCAGTCACTGACGGTTTCGACATGGGGGTGGGCATGCTCACCCGTTTCCTCGGTCGTAACGACACCGAg                                                                                                                       >  2:431988/1‑149 (MQ=255)
                             aCAGGAGTCGTCAAATGATCGATTATGAAGTATTGCGTTTTATCTGGTGGCTGCTGGTTGGCGTTCTGCTGATTGGTTTTGCAGTCACTGACGGTTTCGACATGGGGGTGGGCATGCTCACCCGTTTACTCGGTCGTAACGACACCGAg                                                                                                                       >  4:1592468/1‑149 (MQ=255)
                              cAGGAGTCGTCAAATGATCGATTATGAAGTATTGCGTTTTATCTGGTGGCTGCTGGTTGGCGTTCTGCTGATTGGTTTTGCAGTCACTGACGGTTTCGACATGGGGGTGGGCATGCTCACCCGTTTCCTCGGTCGTAACGACACCGAGc                                                                                                                      <  2:113601/149‑1 (MQ=255)
                              cAGGAGTCGTCAAATGATCGATTATGAAGTATTGCGTTTTATCTGGTGGCTGCTGGTTGGCGTTCTGCTGATTGGTTTTGCAGTCACTGACGGTTTCGACATGGGGGTGGGCATGCTCACCCGTTTCCTCGGTCGTAACGACACCGAGc                                                                                                                      <  3:731568/149‑1 (MQ=255)
                                    tcgtcAAATGATCGATTATGAAGTATTGCGTTTTATCTGGTGGCTGCTGGTTGGCGTTCTGCTGATTGGTTTTGCAGTCACTGACGGTTTCGACATGGGGGTGGGCATGCTCACCCGTTTCCTCGGTCGTAACGACACCGAGCGTCGaa                                                                                                                <  3:1606502/149‑1 (MQ=255)
                                     cgtcAAATGATCGATTATGAAGTATTGCGTTTTATCTGGTGGCTGCTGGTTGGCGTTCTGCTGATTGGTTTTGCAGTCACTGACGGTTTCGACATGGGGGTGGGCATGCTCACCCGTTTCCTCGGTCGTAACGACACCGAGCGTCGAAt                                                                                                               <  1:1274053/149‑1 (MQ=255)
                                      gtcAAATGATCGATTATGAAGTATTGCGTTTTATCTGGTGGCTGCTGGTTGGCGTTCTGCTGATTGGTTTTGCAGTCACTGACGGTTTCGACATGGGGGTGGGCATGCTCACCCGTTTCCTCGGTCGTAACGACACCGAGCGTCGAAtt                                                                                                              >  3:934390/1‑149 (MQ=255)
                                      gtcAAATGATCGATTATGAAGTATTGCGTTTTATCTGGTGGCTGCTGGTTGGCGTTCTGCTGATTGGTTTTGCAGTCACTGACGGTTTCGACATGGGGGTGGGCATGCTCACCCGTTTCCTCGGTCGTAACGACACCGAGCGTCGAAtt                                                                                                              >  1:1253505/1‑149 (MQ=255)
                                       tcAAATGATCGATTATGAAGTATTGCGTTTTATCTGGTGGCTGCTGGTTGGCGTTCTGCTGATTGGTTTTGCAGTCACTGACGGTTTCGACATGGGGGTGGGCATGCTCAccc                                                                                                                                                 <  2:746615/113‑1 (MQ=255)
                                       tcAAATGATCGATTATGAAGTATTGCGTTTTATCTGGTGGCTGCTGGTTGGCGTTCTGCTGATTGGTTTTGCAGTCACTGACGGTTTCGACATGGGGGTGGGCATGCTCACCCGTTTCCTCGGTCGTAACGACACCGAGCGTCGAATTa                                                                                                             >  1:85571/1‑149 (MQ=255)
                                       tcAAATGATCGATTATGAAGTATTGCGTTTTATCTGGTGGCTGCTGGTTGGCGTTCTGCTGATTGGTTTTGCAGTCACTGACGGTTTCGACATGGGGGTGGGCATGCTCACCCGTTTCCTCGGTCGTAACGACACCGAGCGTCGAATTa                                                                                                             >  1:692684/1‑149 (MQ=255)
                                       tcAAATGATCGATTATGAAGTATTGCGTTTTATCTGGTGGCTGCTGGTTGGCGTTCTGCTGATTGGTTTTGCAGTCACTGACGGTTTCGACATGGGGGTGGGCATGCTCACCCGTTTCCTCGGTCGTAACGACACCGAGCGTCGAATTa                                                                                                             >  4:243392/1‑149 (MQ=255)
                                        cAAATGATCGATTATGAAGTATTGCGTTTTATCTGGTGGCTGCTGGTTGGCGTTCTGCTGATTGGTTTTGCAGTCACTGACGGTTTCGACATGGGGGTGAGCATGCTCACCCGTTTCCTCGGTCGTAACGACACCGAGCGTCGAATTAt                                                                                                            <  1:1491200/149‑1 (MQ=255)
                                          aaTGATCGATTATGAAGTATTGCGTTTTATCTGGTGGCTGCTGGTTGGCGTTCTGCTGATTGGTTTTGCAGTCACTGACGGTTTCGACATGGGGGTGGGCATGCTCACCCGTTTCCTCGGTCGTAACGACACCGAGCGTCGAATTATGa                                                                                                          <  1:1527204/149‑1 (MQ=255)
                                            tGATCGATTATGAAGTATTGCGTTTTATCTGGTGGCTGCTGGTTGGCGTTCTGCTGATTGGTTTTGCAGTCACTGACGGTTTCGACATGGGGGTGGGCATGCTCACCCGTTTCCTCGGTCGTAACGACACCGAGCGTCGAATTATGAtt                                                                                                        >  3:1313105/1‑149 (MQ=255)
                                             gATCGATTATGAAGTATTGCGTTTTATCTGGTGGCTGCTGGTTGGCGTTCTGCTGATTGGTTTTGCAGTCACTGACGGTTTCGACATGGGGGTGGGCATGCTCACCCGTTTCCTCGGTCGTAACGACACCGAGCGTCGAATTATGATTa                                                                                                       >  4:1052692/1‑149 (MQ=255)
                                              aTCGATTATGAAGTATTGCGTTTTATCTGGTGGCTGCTGGTTGGCGTTCTGCTGATTGGTTTTGCAGTCACTGACGGTTTCGACATGGGGGTGGGCATGCTCACCCGTTTCCTCGGTCGTAACGACACCGAGCGTCGAATTATGATTaa                                                                                                      <  3:1319050/149‑1 (MQ=255)
                                               tCGATTATGAAGTATTGCGTTTTATCTGGTGGCTGCTGGTTGGCGTTCTGCTGATTGGTTTTGCAGTCACTGACGGTTTCGACATGGGGGTGGGCATGCTCACCCGTTTCCTCGGTCGTAACGACACCGAGCGTCGAATTATGATTAAc                                                                                                     <  4:1293098/149‑1 (MQ=255)
                                                 gATTATGAAGTATTGCGTTTTATCTGGTGGCTGCTGGTTGGCGTTCTGCTGATTGGTTTTGCAGTCACTGACGGTTTCGACATGGGGGTGGGCATGCTCACCCGTTTCCTCGGTCGTAACGACACCGAGCGTCGAATTATGATTAACTc                                                                                                   <  1:548981/149‑1 (MQ=255)
                                                  aTTATGAAGTATTGCGTTTTATCTGGTGGCTGCTGGTTGGCGTTCTGCTGATTGGTTTTGCAGTCACTGACGGTTTCGACATGGGGGTGGGCATGCTCACTCGTTTCCTCGGTCGTAACGACACCGAGCGTCGAATTATGATTAACTcc                                                                                                  <  1:1110306/149‑1 (MQ=255)
                                                   ttATGAAGTATTGCGTTTTATCTGGTGGCTGCTGGTTGGCGTTCTGCTGATTGGTTTTGCAGTCACTGACGGTTTCGACATGGGGGTGGGCATGCTCACCCGTTTCCTCGGTCGTAACGACACCGAGCGTCGAATTATGATTAACTCCa                                                                                                 >  1:1084876/1‑149 (MQ=255)
                                                   ttATGAAGTATTGCGTTTTATCTGGTGGCTGCTGGTTGGCGTTCTGCTGATTGGTTTTGCAGTCACTGACGGTTTCGACATGGGGGTGGGCATGCTCACCCGTTTCCTCGGTCGTAACGACACCGAGCGTCGAATTATGATTAACTCCa                                                                                                 <  1:759282/149‑1 (MQ=255)
                                                       gAAGTATTGCGTTTTATCTGGTGGCTGCTGGTTGGCGTTCTGCTGATTGGTTTTGCAGTCACTGACGGTTTCGACATGGGGGTGGGCATGCTCACCCGTTTCCTCGGTCGTAACGACACCGAGCGTCGAATTATGATTAACTCCAt                                                                                                >  2:455894/1‑146 (MQ=255)
                                                       gAAGTATTGCGTTTTATCTGGTGGCTGCTGGTTGGCGTTCTGCTGATTGGTTTTGCAGTCACTGACGGTTGCGACATGGGGGTGGGCATGCTCACCCGTTTCCTCGGTCGTAACGACACCGAGCGTCGAATTATGATTAACTCCATTGc                                                                                             >  4:45855/1‑149 (MQ=255)
                                                        aaGTATTGCGTTTTATCTGGTGGCTGCTGGTTGGCGTTCTGCTGATTGGTTTTGCAGTCACTGACGGTTTCGACATGGGGGTGGGCATGCTCACCCGTTTCCTCGGTCGTAACGACACCGAGCGTCGAATTATGATTAACTCCATTGca                                                                                            <  1:431988/149‑1 (MQ=255)
                                                          gTATTGCGTTTTATCTGGTGGCTGCTGGTTGGCGTTCTGCTGATTGGTTTTGCAGTCACTGACGGTTTCGACATGGGGGTGGGCATGCTCACCCGTTTCCTCGGTCGTAACGACACCGAGCGTCGAATTATGATTAACTCCATTGcacc                                                                                          <  2:945276/149‑1 (MQ=255)
                                                                   tttATCTGGTGGCTGCTGGTTGGCGTTCTGCTGATTGGTTTTGCAGTCACTGACGGTTTCGACATGGGGGTGGGCATGCTCACCCGTTTCCTCGGTCGTAACGACACCGAGCGTCGAATTATGATTAACTCCATTGCACCACACTGGGa                                                                                 <  2:1253505/149‑1 (MQ=255)
                                                                    ttATCTGGTGGCTGCTGGTTGGCGTTCTGCTGATTGGTTTTGCAGTCACTGACGGTTTCGACATGGGGGTGGGCATGCTCACCCGTTTCCTCGGTCGTAACGACACCGAGCGTCGAATTATGATTAACTCCATTGCACCACACTGGGAc                                                                                <  3:553341/149‑1 (MQ=255)
                                                                    ttATCTGGTGGCTGCTGGTTGGCGTTCTGCTGATTGGTTTTGCAGTCACTGACGGTTTCGACATGGGGGTGGGCATGCTCACCCGTTTCCTCGGTCGTAACGACACCGAGCGTCGAATTATGATTAACTCCATTGCACCACACTGGGAc                                                                                <  2:918518/149‑1 (MQ=255)
                                                                         tggtggCTGCTGGTTGGCGTTCTGCTGATTGGTTTTGCAGTCACTGACGGTTTCGACATGGGGGTGGGCATGCTCACCCGTTTCCTCGGTCGTAACGACACCGAGCGTCGAATTATGATTAACTCCATTGCACCACACTGGGACGGTaa                                                                           <  3:1090932/149‑1 (MQ=255)
                                                                           gtggCTGCTGGTTGGCGTTCTGCTGATTGGTTTTGCAGTCACTGACGGTTTCGACATGGGGGTGGGCATGCTCACCCGTTTCCTCGGTCGTAACGACACCGAGCGTCGAATTATGATTAACTCCATTGCACCACACTGGGACGGTAAcc                                                                         >  1:1281263/1‑149 (MQ=255)
                                                                             ggCTGCTGGTTGGCGTTCTGCTGATTGGTTTTGCAGTCACTGACGGTTTCGACATGGGGGTGGGCATGCTCACCCGTTTCCTCGGTCGTAACGACACCGAGCGTCGAATTATGATTAACTCCATTGCACCACACTGGGACGGTAACCAg                                                                       <  1:1517642/149‑1 (MQ=255)
                                                                               ctgctgGTTGGCGTTCTGCTGATTGGTTTTGCAGTCACTGACGGTTTCGACATGGTGGTGGGCATGCTCACCCGTTTCCTCGGTCGTAACGACACCGAGCGTCGAATTATGATTAACTCCATTGCACCACACTGGGACGGTAACCAGGt                                                                     <  3:294027/149‑1 (MQ=255)
                                                                                tgctgGTTGGCGTTCTGCTGATTGGTTTTGCAGTCACTGACGGTTTCGACATGGGGGTGGGCATGCTCACCCGTTTCCTCGGTCGTAACGACACCGAGCGTCGAATTATGATTAACTCCATTGCACCACACTGGGACGGTAACCAGGtt                                                                    >  4:796611/1‑149 (MQ=255)
                                                                                  ctgGTTGGCGTTCTGCTGATTGGTTTTGCAGTCACTGACGGTTTCGACATGGGGGTGGGCATGCTCACCCGTTTCCTCGGTCGTAACGACACCGAGCGTCGAATTATGATTAACTCCATTGCACCACACTGGGACGGTAACCAGGTTTg                                                                  <  2:855119/149‑1 (MQ=255)
                                                                                   tgGTTGGCGTTCTGCTGATTGGTTTTGCAGTCACTGACGGTTTCGACATGGGGGTGGGCATGCTCACCCGTTTCCTCGGTCGTAACGACACCGAGCGTCGAATTATGATTAACTCCATTGCACCACACTGGGACGGTAACCAGGTTTgg                                                                 <  2:512935/149‑1 (MQ=255)
                                                                                    ggTTGGCGTTCTGCTGATTGGTTTTGCAGTCACTGACGGTTTCGACATGGGGGTGGGCATGCTCACCCGTTTCCTCGGTCGTAACGACACCGAGCGTCGAATTATGATTAACTCCATTGCACCACACTGGGACGGTAACCAGGTTTGGc                                                                <  1:1598986/149‑1 (MQ=255)
                                                                                    ggTTGGCGTTCTGCTGATTGGTTTTGCAGTCACTGACGGTTTCGACATGGGGGTGGGCATGCTCACCCGTTTCCTCGGTCGTAACGACACCGAGCGTCGAATTATGATTAACTCCATTGCACCACACTGGGACGGTAACCAGGTTTGGc                                                                <  2:1336721/149‑1 (MQ=255)
                                                                                    ggTTGGCGTTCTGCTGATTGGTTTTGCAGTCACTGACGGTTTCGACATGGGGGTGGGCATGCTCACCCGTTTCCTCGGTCGTAACGACACCGAGCGTCGAATTATGATTAACTCCATTGCACCACACTGGGACGGTAACCAGGTTTGGc                                                                <  3:243392/149‑1 (MQ=255)
                                                                                        ggCGTTCTGCTGATTGGTTTTGCAGTCACTGACGGTTTCGACATGGGGGTGGGCATGCTCACCCGTTTCCTCGGTCGTAACGACACCGAGCGTCGAATTATGATTAACTCCATTGCACCACACTGGGACGGTAACCAGGTTTGGCTGAt                                                            >  1:1688979/1‑149 (MQ=255)
                                                                                               tgctgATTGGTTTTGCAGTCACTGACGGTTTCGACATGGGGGTGGGCATGCTCACCCGTTTCCTCGGTCGTAACGACACCGAGCGTCGAATTATGATTAACTCCATTGCACCACACTGGGACGGTAACCa                                                                        <  4:322850/130‑1 (MQ=255)
                                                                                               tgctgATTGGTTTTGCAGTCACTGACGGTTTCGACATGGGGGTGGGCATGCTCACCCGTTTCCTCGGTCGTAACGACACCGAGCGTCGAATTATGATTAACTCCATTGCACCACACTGGGACGGTAACCAGGTTTGGCTGATCACcgcg                                                     >  3:1250367/1‑149 (MQ=255)
                                                                                                gctgATTGGTTTTGCAGTCACTGACGGTTTCGACATGGGGGTGGGCATGCTCACCCGTTTCCTCGGTCGTAACGACACCGAGCGTCGAATTATGATTAACTCCATTGCACCACACTGGGACGGTAACCAGGTTTGGCTGATCACCgcgg                                                    <  1:205414/149‑1 (MQ=255)
                                                                                                  tgATTGGTTTTGCAGTCACTGACGGTTTCGACATGGGGGTGGGCATGCTCACCCGTTTCCTCGGTCGTAACGACACCGAGCGTCGAATTATGa                                                                                                          >  4:353177/1‑93 (MQ=255)
                                                                                                  tgATTGGTTTTGCAGTCACTGACGGTTTCGACATGGGGGTGGGCATGCTCACCCGTTTCCTCGGTCGTAACGACACCGAGCGTCGAATTATGa                                                                                                          <  3:353177/93‑1 (MQ=255)
                                                                                                  tgATTGGTTTTGCAGTCACTGACGGTTTCGACATGGGGGTGGGCATGCGCACCCGTTTCCTCGGTCGTAACGACACCGAGCGTCGAATTATGATTAACTCCATTGCACCACACTGGGACGGTAACCAGGTTTGGCTGATCACCgcgggc                                                  >  1:1109621/1‑149 (MQ=255)
                                                                                                    aTTGGTTTTGCAGTCACTGACGGTTTCGACATGGGGGTGGGCATGCTCACCCGTTTCCTCGGTCGTAACGACACCGAGCGTCGaa                                                                                                                <  1:1227688/85‑1 (MQ=255)
                                                                                                    aTTGGTTTTGCAGTCACTGACGGTTTCGACATGGGGGTGGGCATGCTCACCCGTTTCCTCGGTCGTAACGACACCGAGCGTCGaa                                                                                                                >  2:1227688/1‑85 (MQ=255)
                                                                                                    aTTGGTTTTGCAGTCACTGACGGTTTCGACATGGGGGTGGGCATGCTCACCCGTTTCCTCGGTCGTAACGACACCGAGCGTCGAATTACGATTAACTCCATTGCACCACACTGGGACGGTAACCAg                                                                       <  1:868883/126‑1 (MQ=255)
                                                                                                     ttGGTTTTGCAGTCACTGACGGTTTCGACATGGGGGTGGGCATGCTCACCCGTTTCCTCGGTCGTAACGACACCGAGCGTCGAATTATGATTAACTCCATTGCACCACACTgg                                                                                   >  3:1274829/1‑113 (MQ=255)
                                                                                                      tGGTTTTGCAGTCACTGACGGTTTCGACATGGGGGTGGGCATGCTCACCCGTTTCCTCGGTCGTAACGACACCGAGCGTCGAATTATGATTAACTCCATTGCACCACACTGGGACGGTAACCAGGTTTGGCTGATCACCGCGGgcggcg                                              >  4:599828/1‑149 (MQ=255)
                                                                                                             gCAGTCACTGACGGTTTCGACATGGGGGTGGGCATGCTCACCCGTTTCCTCGGTCGTAACGACACCGAGCGTCGAATTATGATTAACTCCATTGCACCACACTGGGACGGTAACCAGGTTTGGCTGATCACCGCGGGCGGCGCACTCtt                                       <  1:361727/149‑1 (MQ=255)
                                                                                                               aGTCACTGACGGTTTCGACATGGGGGTGGGCATGCTCACCCGTTTCCTCGGTCGTAACGACACCGAGCGTCGAATTATGATTaa                                                                                                      <  2:1485329/84‑1 (MQ=255)
                                                                                                                gTCACTGACGGTTTCGACATGGGGGTGGGCATGCTCACCCGTTTCCTCGGTCGTAACGACACCGAGCGTCGAATTATGATTAACTCCATTGCACCACACTGGGACGGTAACCAGGTTTGGCTGATCACCGCGGGCGGCGCACTCTTtgc                                    <  1:648974/149‑1 (MQ=255)
                                                                                                                 tCACTGACGGTTTCGACATGGGGGTGGGCATGCTCACCCGTTTCCTCGGTCGTAACGACACCGAGCGTCGAATTATGATTAACTCCATTGCACCACACTGGGACGGTAACCAGGTTTGGCTGATCACCGCGGGCGGCGCACTCTTtgct                                   >  2:634313/1‑149 (MQ=255)
                                                                                                                 tCACTGACGGTTTCGACATGGGGGTGGGCATGCTCACCCGTTTCCTCGGTCGTAACGACACCGAGCGTCGAATTATGATTAACTCCATTGCACCACACTGGGACGGTAACCAGGTTTGGCTGATCACCGCGGGCGGCGCACTCTTtgct                                   <  1:1507318/149‑1 (MQ=255)
                                                                                                                     tGACGGTTTCGACATGGGGGTGGGCATGCTCACCCGTTTCCTCGGTCGTAACGACACCGAGCGTCGAATTATGATTAACTCCATTGCACCACACTGGGACGGTAACCAGGTTTGGCTGATCACCGCGGGCGGCGCACTCTTTGCTGCCt                               >  1:1464761/1‑149 (MQ=255)
                                                                                                                     tGACGGTTTCGACATGGGGGTGGGCATGCTCACCCGTTTCCTCGGTCGTAACGACACCGAGCGTCGAATTATGATTAACTCCATTGCACCACACTGGGACGGTAACCAGGTTTGGCTGATCACCGCGGGCGGCGCACTCTTTGCTGCCt                               >  4:1672221/1‑149 (MQ=255)
                                                                                                                     tGACGGTTTCGACATGGGGGTGGGCATGCTCACCCGTTTCCTCGGTCGTAACGACACCGAGCGTCGAATTATGATTAACTCCATTGCACCACACTGGGACGGTAACCAGGTTTAGCTGATCACCGCGGGCGGCGCACTCTTTGCTGCCt                               <  1:919286/149‑1 (MQ=255)
                                                                                                                       aCGGTTTCGACATGGGGGTGGGCATGCTCACCCGTTTCCTCGGTCGTAACGACACCGAGCGTCGAATTATGATTAACTCCATTGCACCACACTGGGACGGTAACCAGGTTTGGCTGATCACCGCGGGCGGCGCACTCTTTGCTGCCTgg                             >  1:1217011/1‑149 (MQ=255)
                                                                                                                        cGGTTTCGACATGGGGGTGGGCATGCTCACCCGTTTCCTCGGTCGTAACGACACCGAGCGTCGAATTATGATTAACTCCATTGCACCACACTGGGACGGTAACCAGGTTTGGCTGATCACCGCGGGCGGCGCACTCTTTGCTGCCTGGc                            <  2:1569629/149‑1 (MQ=255)
                                                                                                                           tttCGACATGGGGGTGGGCATGCTCACCCGTTTCCTCGGTCGTAACGACACCGAGCGTCGAATTATGATTAACTCCATTGCACCACACTGGGACGGTAACCAGGTTTGGCTGATCACCGCGGGCGGCGCACTCTTTGCTGCCTGGCCGa                         >  2:729965/1‑149 (MQ=255)
                                                                                                                           tttCGACATGGGGGTGGGCATGCTCACCCGTTTCCTCGGTCGTAACGACACCGAGCGTCGAATTATGATTAACTCCATTGCACCACACTGGGACGGTAACCAGGTTTGGCTGATCACCGCGGGCGGCGCACTCTTTGCTGCCTGGCCGa                         >  4:860893/1‑149 (MQ=255)
                                                                                                                             tCGACATGGGGGTGGGCATGCTCACCCGTTTCCTCGGTCGTAACGACACCGAGCGTCGAATTATGATTAACTCCATTGCACCACACTGGGACGGTAACCAGGTTTGGCTGATCACCGCGGGCGGCGCACTCTTTGCTGCCTGGCCGATg                       <  2:845786/149‑1 (MQ=255)
                                                                                                                               gACATGGGGGTGGGCATGCTCACCCGTTTCCTCGGTCGTAACGACACCGAGCGTCGAATTATGATTAACTCCATTGCACCACACTGGGACGGTAACCAGGTTTGGCTGATCACCGCGGGCGGCGCACTCTTTGCTGCCTGGCCGATGGt                     >  3:1678025/1‑149 (MQ=255)
                                                                                                                                  aTGGGGGTGGGCATGCTCACCCGTTTCCTCGGTCGTAACGACACCGAGCGTCGAATTATGATTAACTCCATTGCACCACACTGGGACGGTAACCAGGTTTGGCTGATCACCGCGGGCGGCGCACTCTTTGCTGCCTGGCCGATGGTCTa                  >  2:1556327/1‑149 (MQ=255)
                                                                                                                                            gCATGCTCACCCGTTTCCTCGGTCGTAACGACACCGAGCGTCGAATTATGATTAACTCCATTGCACCACACTGGGACGGTAACCAGGTTTGGCTGATCACCGCGGGCGGCGCa                                            <  2:306955/113‑1 (MQ=255)
                                                                                                                                            gCATGCTCACCCGTTTCCTCGGTCGTAACGACACCGAGCGTCGAATTATGATTAACTCCATTGCACCACACTGGGACGGTAACCAGGTTTGGCTGATCACCGCGGGCGGCGCa                                            >  1:306955/1‑113 (MQ=255)
                                                                                                                                                gCTCACCCGTTTCCTCGGTCGTAACGACACCGAGCGTCGAATTATGATTAACTCCATTGc                                                                                             >  4:290828/1‑60 (MQ=255)
                                                                                                                                                gCTCACCCGTTTCCTCGGTCGTAACGACACCGAGCGTCGAATTATGATTAACTCCATTGc                                                                                             <  3:290828/60‑1 (MQ=255)
                                                                                                                                                 cTCACCCGTTTCCTCGGTCGTAACGACACCGAGCGTCGAATTATGATTAACTCAATTGCACCACACTGGGACGGTACCCAGGTTTGGCTGATCACCGCGGGCGGCGCACTCTTTGCTGCCTGGCCGATGGTCTATGCCGCTGCGCtctc   <  2:550585/149‑1 (MQ=255)
                                                                                                                                                  tCACCCGTTTCCTCGGTCGTAACGACACCGAGCGTCGAATTATGATTAACTCCATTGCACCACACTAGGACGGTAACCAGGTTTGGCTGATCACCGCGGGCGGCGCACTCTTTGCTGCCTGGCCGATGGTCTATGCCGCTGGGTTCTcc  <  4:1425922/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
TTCCACGACTACTCAGCCGGCACGCTAAGACAGGAGTCGTCAAATGATCGATTATGAAGTATTGCGTTTTATCTGGTGGCTGCTGGTTGGCGTTCTGCTGATTGGTTTTGCAGTCACTGACGGTTTCGACATGGGGGTGGGCATGCTCACCCGTTTCCTCGGTCGTAACGACACCGAGCGTCGAATTATGATTAACTCCATTGCACCACACTGGGACGGTAACCAGGTTTGGCTGATCACCGCGGGCGGCGCACTCTTTGCTGCCTGGCCGATGGTCTATGCCGCTGCGTTCTCC  >  NC_000913/772999‑773293

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: