Predicted mutation | ||||||
---|---|---|---|---|---|---|
evidence | seq id | position | mutation | annotation | gene | description |
RA | NC_000913 | 4,296,381 | +GC | intergenic (+587/+55) | gltP → / ← yjcO | glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 4,296,380 | 1 | . | C | 98.0% | 281.2 / ‑1.3 | 103 | intergenic (+586/+56) | gltP/yjcO | glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein |
Reads supporting (aligned to +/- strand): ref base . (1/1); new base C (56/44); total (58/45) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.19e-01 | |||||||||||
* | NC_000913 | 4,296,380 | 2 | . | G | 95.0% | 265.5 / NA | 103 | intergenic (+586/+56) | gltP/yjcO | glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein |
Reads supporting (aligned to +/- strand): ref base . (1/1); major base G (57/39); minor base T (0/5); total (58/45) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.37e-02 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01 |
Only 100 of 103 total aligned reads displayed.
TCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑A‑‑C‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAC‑CCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTGCGTTTGAAATACCAGGTTGCCTTGT > NC_000913/4296162‑4296638 || tCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGACGCGTCTTATCCGGCCTACGCCAGGCCGCGCAATAGCCTTATCTAGCGTCAGTTGGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCAACCCCAATCAATGCCTTATGCGCCGCTTGCCGCGTCGTTTCGGGCCTac > 3:312833/1‑274 (MQ=11) gATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACCTCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACTCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTCGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCACTGCTTGATTCGACGCTTACCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTa > 1:617340/1‑274 (MQ=11) cGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTAAGCCAGACAGCGAAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGAAGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTACATTTTcc > 2:563433/1‑274 (MQ=11) gccgcgTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAAct > 3:121062/3‑274 (MQ=255) ttATCAGACCTACCCCAGACAGCGAAATAGACTGATTTAGCGTTATTTTTTAGGTCTGATAAGGCGTTTATTCCGCATCATACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAc‑c < 2:375517/275‑1 (MQ=14) gtACGCGCGACAGGTCAATAGCCTGTGTTAGCGTGATTTTGTAGGTCGGAT‑AGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGAAGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAC‑CCGGTATCaaa < 1:422970/274‑1 (MQ=14) tACTCCAGACACCGAAATAGCCTTATTTAGGGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATTCGACGCTTAACTCGTCTTATCAGGCTTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAC‑CCGGTATCaaa < 2:664494/275‑1 (MQ=14) cGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCTTCTTATCAGGCCTACCCCAGACAGCGCAATAGCCTGCTTTCGCGTGATTTTGTATGTCGGATAAGGCGTTC‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGTCGCTTGCCGCTTCTTATAAGCCCTATCTTAACCGTAGGTTAATTTTTAAAACCCTTTACAC‑CCGGtcacaatcc > 3:402067/1‑267 (MQ=14) cGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGCCTTATCAGGCTTATCTTAACCGTTGGTTAATTTTTCCAACTCTTCCCAC‑CCGGTATTAAAcc > 2:150483/1‑275 (MQ=255) gCCAGACAGCGCAATAGCCTGATTTAGCTTGATTTTGTAGGTCGGATAAGGCGTGTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAC‑CCGGTATCAAAccc < 2:504410/275‑1 (MQ=255) gACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGTATAAGGCGTTT‑‑ACGC‑C‑GCACCTGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGCTCTATCTTAACTGTTTGTAACTTTTTCAAACTCTTCACAC‑CCGTTATAAAAcctctc > 3:292014/1‑270 (MQ=14) aGCGCAATAGCCTGAGTTAGCGTGATTGTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGGCAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAC‑CCGGTATCAAACCCTTCCATa < 1:607338/275‑1 (MQ=255) cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATCGCCTGATTTAGCGTGATTTTGTAGGTCGGATACGGCGTTT‑‑ACGC‑C‑GCATCCGACATCACTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTCACCGTTGGTTAATTTTTCCAACTCTTCACAC‑CCGGTAGCAAACTCTTccttcc > 2:579798/1‑270 (MQ=14) cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATTAGGCCTACCTTAACCGTCGGTTAATTTTTCAAATCCTTCACAC‑CCGGTGTCAAACCCTTCCATa > 1:303548/1‑273 (MQ=14) cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAC‑CCGGTATCAAACCCTTCCATa < 2:443651/273‑1 (MQ=255) cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAC‑CCGGTATCAAACCCTTCCATa < 1:121062/273‑1 (MQ=255) cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAC‑CCGGTATCAAACCCTTCCATAc > 1:520658/1‑274 (MQ=255) cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATATTGTCGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATTCCTGATTCGACCCTCGCCGTGTCGCATCAGTCTTATCTTAACCGTTGGCTACTTTTTACAACTCATCCCAC‑CCGGTAGCAAATCCTTCCATACa > 3:248865/1‑275 (MQ=14) cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTGAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAATTCTTCACCC‑CCTGTTTCAAACCCTTcactaaa > 1:273022/1‑269 (MQ=14) tAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGCTTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATTCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACCC‑CCTGTATCACACCCTTCCATACAGCTc > 3:687981/1‑274 (MQ=255) tAGCCTGATTTAGCGTGATTTTGTAGGTCGGAGAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAC‑CCGGTATCAAACCCTTCCATACAGCTCa < 2:53384/275‑1 (MQ=255) ccttttttAGCGTGATTGTGTAGGGCGGATAAGGGGTTTCTGCCGCATCCGGCATCAACGCCTGCTGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACGGCGCAATAGCCTGATTTAGCGTGATTTTTTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACACCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAC‑CCGGTATCAACCCCTTCCATACAGCTCAGGt < 1:494357/270‑1 (MQ=17) ccTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATCGCCTGATTTAGCGTGTTTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCGATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAA‑CCGGTATCAAATCCTTCCATCTAGCTCAgg > 3:728237/1‑274 (MQ=17) tGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCTATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCCCAC‑CCGTTATCAACCCCTTCCATCCATCTCAGGtt > 2:515310/1‑274 (MQ=17) tGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAC‑CCGGTATCAAACCCTTCCTTCCAGCTCAGttt > 1:6784/1‑274 (MQ=255) tGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAC‑CCGGTATCAAACCCTTCCATACAGCTCAGGtt > 2:408981/1‑274 (MQ=255) tGATTAAGCATAATTTTATAGGTCGGATAAGGCATTTATGCGGCATCCGACATCAACGCCGGATGCGACGCGTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATGGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAC‑CCGGTATCAAACCCTTCCATACAGCTCAGGTTc < 1:728237/275‑1 (MQ=17) gATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACCTCAATGCCTGATGCGACGACGGCCGCTTCTTCTCAGGCCTATCTTCACCGTTGGTTAATTTTTCAAACCCTTca > 3:731396/1‑238 (MQ=14) gATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGGTAATTTTTCAAACTCTTCACAC‑CCGGTATCAAACCCTTCCATACCGCTCAGGtt > 1:493325/1‑273 (MQ=255) gtAGCGTGATTTTGTCAGTCGGATAAGGCGTTTCTGCCGCATCCGGAATCAACACCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCGTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGATTAATTTTTCAAACTCTTCACAC‑CCGGTATCAAACCCTTCCATACAGCTCAGGTTCAAc < 1:741347/273‑1 (MQ=17) taggatGATTTGGTAAGTCGGTTAGAGCGTTTATTCCGCATCCGACATCAACGTCTGGTGCGACGCTTAACGCGTGTTAGCAGGCCTACGCCATACAGCGCAATCGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAC‑CCGGTATCAAACCCTTCCATACAGCTCAGGTTCAAc < 3:543117/268‑1 (MQ=18) cGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCGGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTCCG‑‑C‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTCACCTTTGGTTAATTTTTCAGATTCCTCACCC‑CCGGTATCAAACCCCTCCATACTACCCAGGTTCAAcaactt > 3:801856/1‑270 (MQ=14) cGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCTGACATCAATGTCCGATGCGACGCTTGCCGCCTCTTATCAGGCCTATCTTAACACTTGGTTAATTTTTCAAATCCTTC‑CACTCCGGTATCCAACCCTTCCATACAGTCCTCGTTCAACCattc > 3:401733/1‑272 (MQ=14) cGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAC‑CCGGTATCAAACCCTTGCTTACAGCACAGGTTCAACCattt > 2:349887/1‑272 (MQ=255) aTTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCCCAC‑CCGGTATCAAACCATTCCATACAGCCCAGGTTCACCCAGTGCAgc > 2:604170/1‑275 (MQ=255) tAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCTACGCTTGCCGCGTCGTCTCACGCCTACCTTAACCGTGGGTTAATTTTTCAAACTCTTCACAC‑CCGGTGTCAAACCCTTCCATACCTCTTCGGTTAAACCATTgaaactcgtt > 3:557944/1‑265 (MQ=14) cGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTACTTTTTCAAACTCTTCACAC‑CCGGTATCAAACCCTTCCATCCCTCTCATGTTCAACCAGTTCCGCCCCTTTTGCtt > 2:141072/1‑275 (MQ=17) aGGCGTTGATTACGCATCAGAAATCAACGCATGATGCGACGCTTAATGCGTCTTATCAGGCCTACGCCAGACGGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTG‑‑ACGC‑C‑GCATCCGACATCAATGCCTGCTGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAC‑CCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGttctt < 3:248102/275‑1 (MQ=21) aGGAGTTTTTGCCCTATCCAACATCCACGCCTTATCCGACGCTTAAGGAGTATTAGCAGGCCTAAGCCAGACCGCGCAATAGCCTGATTTAGCGTGATTTTGTGTGTCGGATAGGGCGTTT‑‑ACGC‑C‑GCGTCCGACATCAATGCGTGATGCTACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAC‑CCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGttctt < 3:276570/275‑1 (MQ=255) gCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTCACAGTTGGTTAATTTTTCAAACTCTTCACAC‑CCGGTATCAAACCCTTCCATACAGCTCAGTTTCACCCATTTCACCGCCTTTTTCTTCTtcctct > 3:17798/1‑275 (MQ=17) cGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTTATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATCCGACGCTTGCCGCGTCTTAGCAGGCCTATCTTAACCTTTGGTTAATTTTTCAAACTCTTCCAAC‑CACGTATCAAACCCTTCCATACAGCTCAGGGTCACCCAGCACAGCGCCTTTTTCttctcattc > 2:303121/1‑266 (MQ=17) tATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATACGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTCTCTTAACCATTGGTTATGTTTTCAAACCCTTCACAC‑CCGTTTTCTAACTCTTTCATTCATCTATGTATCACCTACTGCTGCCCCTTTCGATTGTTCTTCTCgct > 4:640604/1‑272 (MQ=14) tATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGCCGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAC‑CCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTACCGCGCCTTTTGCTTGTtcttctt > 3:169453/1‑270 (MQ=255) tATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGAGTCTTGCCGCGTCTTATCATGCCTCTCTTACCCGTTGGTTAATTTTTCATCCTCTTCACCC‑CCGCTTCCACCCCCTTCCTTACAGCTCGCGTTCAACCTGTGCCGCCCCTTTTGCTTGttcattcctat > 4:204542/1‑266 (MQ=14) tATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCATAC‑CCGGTATCAAACCCTTCCATACAGCTCAGGTTAAACCAGTCCCGCGCCCTCTGCTTGTTCTTCTCGAt > 3:422598/1‑274 (MQ=18) tATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAC‑CCGGTAGCAACCCCTTCCATACAGCCCAGGTTCAACCAGCGCAGCGCCTTTTGCTTGTTCTTCCCGATa > 3:124132/1‑275 (MQ=255) ccGCATCCGCCATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGGCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAC‑CCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATa < 1:169453/271‑1 (MQ=255) cGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGCTGGTTAATTTTTCAAACCCTTCACAC‑CCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCGTTGCAGAGCCTTTTGATTGTTCTTCCCGATAAAAcc > 1:418294/1‑275 (MQ=18) gCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAC‑CCGGTATCAAACCCTTCCATACATCTCAGGTTCAACCCGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAACACct > 2:206780/1‑275 (MQ=255) ccagcATCAACCGCTGATGAGACGCTGAACGCGTCTTCTCAGGCCGACGCCAGAGAACGCAATAGCCTGATTGCGGGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACTC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAC‑CCGGTATAAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACcttctt < 2:639448/271‑1 (MQ=25) ggcATCAAAGCCTGATCCGACGCTTTAGGCGTCTTATCAAGCCTAAGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGAAATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAC‑CCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCttcttc < 1:17798/272‑1 (MQ=25) tcatccccTGATGCTACGCTTAACGCGTCTTATAAGGACTAAACCAGAAAGAGCAATAGCCTTATTTAGCTGGATTGAGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAC‑CCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGt < 2:163204/269‑1 (MQ=25) cGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAC‑CCGGTATCAAACCCTTCCATACCGCTCAGGTTCAACCATTTCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTa > 1:446216/1‑274 (MQ=255) tGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAC‑CCGGTATCAAACCCTTCCATACAGCTCAGTTTCAACCAGTGCATCGCCTTTCTCTTGTTCTTCTCGTTAAAACCTTCTTCACCgctgtccct > 3:524156/1‑266 (MQ=21) gCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAC‑CCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAAc < 1:524156/251‑1 (MQ=255) accacttatgcgTCGTATCATGGCTAGGGCAGACAGCGCAATAGCCTGATATCGGGAGATTTTGATGGTCCGATCAGTCGTTT‑‑ACGC‑C‑GCATTCGACATCAATGCATGATACGACGCTTGCCGCGTCTGATCAGGCCTATCATAACCATTGGTTAATTTTGCTAGCTCTTCACAC‑CCGGTATCAAACCCTTCCCTTCAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCGTCACCGTTTAAGAACATCATTc < 3:446216/266‑1 (MQ=255) aCGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAC‑CCGGTATCAAATCCTTCCATACAGCTCAGGTTCAACCAGTGCTGCGCCTTTTGCTTGTTCTTTTCGATAAAACCTTTTTCACCGTTTAAGAACCTTAttt > 1:441729/1‑274 (MQ=25) ctaccgggttttgtCAGGCACACGCCAGCCAGCGCTATGTCCTTATTTAGCGTGATTTTTTAGGTCGGAGAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATTCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCCAACTCTTCACAC‑CCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGc < 4:237388/262‑1 (MQ=37) taccgcgTGTTTTCGGGCCTACGACATACAGCTCATTGTCCGTATTTCGAGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACTC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAC‑CCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGcc < 4:604170/272‑1 (MQ=37) tAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAC‑CCGGTATCAAACCCTTCCATACAGCTCTGGTTCAACCAGTGCAGCGCCTTTTGCTTTTTCTTCTCCTTAAAACCTTCCTCACCGTTTAACAACATCATTCCCTcc > 3:137564/1‑275 (MQ=25) gaaCGCGTCTGTTCCGGCCTCCGCCAGACAACGCAATAGCGTGATTTAGCGTGATTTGTTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAC‑CCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGcc < 3:449707/274‑1 (MQ=37) aaCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAC‑CCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGccc < 2:80064/275‑1 (MQ=255) ttATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAC‑CCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCg > 1:523204/1‑275 (MQ=255) gcttaggCCAGAAGGCGAAATGGCCTGTTTGATGGTGATTTTGTAGGTCGGATAATGCGTTT‑‑A‑‑CGCTGCATCCGGCATCAAGGCCTGATGCGACGCTTGCCGCGTCGTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAC‑CCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCGTTTGCTTGTTCTTCTCGATAAAGCCGTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAAc < 3:737752/269‑1 (MQ=255) gCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAC‑CCGGTATCAAACCCTTCCATACAGCTCCGGTTCAACCAGTTCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAAAACATCATTCCCGCCCCGTCTCCGGAATAAc > 4:441505/1‑275 (MQ=37) gCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAC‑CCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCCGCGCCTTTTTCTTGTTCTTCTCGTTAAAACCTTTTTCATCGTTAAAGAACATCATCCCCGCCCAGTTATCGTAATAAc > 4:382619/1‑275 (MQ=25) gCCTACGCCAGACAGCGCAATAGCCTGATGTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACGCTTCACAC‑CCGGTATCAAAACCTTCCATACAGCTCAGGTTAAACCAGTACAGACCCTTTCTCTTTTTCTTACCGATAAAACCTTACTCACCGGTTCAGCACTGCTTTCCCGCTCACTTCTCGGAATAAc > 3:388096/1‑275 (MQ=18) cGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACCCTTCACAC‑CCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCTTTTAATACCATCATTCCCGCCCCTTACTCGGAATAACCGGtt > 3:783214/1‑275 (MQ=37) cGCCAGACAGCGCAATAGCCTGACTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAC‑CCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCCCGATAACACCTTCTTCACCGTTTAATAACATCATTCCCCTCCAGTACCCCGAATACCCCGtc > 4:189167/1‑274 (MQ=25) gCCAGACCGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTATTATCATGCCTATCTTAACCGTTTGTTAATTTTTCAAACCCTTCACAC‑CCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGtt < 1:312833/274‑1 (MQ=255) aGACAGCGCAATAGCCTGCCTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCTACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAC‑CCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCg < 2:189167/259‑1 (MQ=255) gACACCGCAAGTGCCTGATTTAGCGTTATTTTGTAGGTCGGCTAAGGCGTTT‑‑ACTC‑C‑CCATCCGTCATCAATTCCTTATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAC‑CCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGa < 1:248865/275‑1 (MQ=255) aGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ATGC‑C‑GCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAC‑CCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCCGTCCTCGGAATACCCGGTTCGGGAAAt > 2:418355/1‑275 (MQ=37) cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAC‑CCGGTATCAAACCCTTCCATACAGCTCAGGtt > 1:520465/1‑171 (MQ=255) cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAC‑CCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCATTACATCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGCACATCATTCCCGCCCAGTACTCGGAATAACCGTTTCGGAAACTTg > 4:117658/1‑275 (MQ=37) cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAC‑CCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTCCACCGTTTAAGAAAATCATTCCCGCCCAGTACCCGAAATAACCGGTTCGGCATAttt > 1:537423/1‑274 (MQ=37) cgcAAGAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAC‑CCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGcc < 2:406330/244‑1 (MQ=255) aTAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAC‑CCGGTATCAAACCCTTCCATACAGCTCAGGTTCAAc < 3:520465/171‑1 (MQ=255) tAGCCTGGTTTAGCGTGATGTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTGCACTC‑CCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGCagag < 1:773428/275‑1 (MQ=255) tAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAC‑CCGGTATCAAACCCTTCCATACATCTCAGGTTCAACTAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACATTCTTCACCGTTTAAGAACTTCATTctccacca > 3:651075/1‑234 (MQ=37) gCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAC‑CCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACcttct > 2:586496/1‑210 (MQ=255) accTGTTTTTGCGTGATTTTGTAGGTCGGTTCAGTCCTTT‑‑ACGC‑C‑TCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAC‑CCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGCAGAGCt < 2:550512/274‑1 (MQ=39) ccTGACTTAGCGCGATTTTGTAGGTCGGATAAGGCGTAT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAC‑CCGGTATCAAACCCTTCCATCCAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAATctt > 1:177190/1‑207 (MQ=37) cTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCTTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCACACTCTTCACAC‑CCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTAc < 1:651075/241‑1 (MQ=255) gATGTAGCTTGATTTTCTCGGTCGGATAACGCGTTT‑‑ACTC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAC‑CCGGTATCAAACCCCTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTGCGt < 4:303121/275‑1 (MQ=39) aTTTAGCGTGATTTTGTAGGTCGGCTAAGGCGTAT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATTCGACGCTTTCCGCGTCTTATCAGACCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAC‑CCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCttcttc < 3:177190/207‑1 (MQ=255) aTTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAC‑CCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTGCGtt > 3:735821/1‑275 (MQ=255) gttAGCTTGATTTTGTAGGGCGGATAAGGCTTTT‑‑ACGC‑C‑GCATCCGAAATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAC‑CCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTGCGtt < 4:408981/273‑1 (MQ=255) tggCGTGATTTTGTAGTGCGGATAAGGCTTTT‑‑ACGC‑C‑GCATCCGACATCTATTCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAC‑CCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTGCGTTTg < 4:515310/272‑1 (MQ=255) tAGCGTGATTTTTGCGGTAGTATAAGGCGTTT‑‑ACGC‑C‑ACATCCTGATTCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAC‑CCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTGc < 1:596623/269‑1 (MQ=255) tAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAC‑CCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTGCGTTTg < 1:10381/274‑1 (MQ=255) tAGCGTGACTTGTTAGGTCTGATAAGGCGCTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAC‑CCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTGCGttt < 2:63554/273‑1 (MQ=255) cGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAC‑CCGGTATCAAACCCTTCCCTACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTCCTTGTTCTTCTCGATACAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATACCCGGTTCGGGAAATTGCAGAGCTGCGTTTGaa > 3:91009/1‑273 (MQ=255) tttttGTAGGTCGGGTAGGGCGTTT‑‑ACGC‑C‑GAATCCGACATCAATCCCTGATGCGACTCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAC‑CCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTGCGTTTGAAATAcc < 3:520658/273‑1 (MQ=255) tAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTTCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAC‑CCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTGCGTTTGAAATACCAGGTTGc < 1:687981/275‑1 (MQ=255) agggggtagaaGGCGTTT‑‑ACGC‑C‑GCATTCTACATCCATGCCTGATTCGACGCTTGCCGCCTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCCAACTCTTCACAC‑CCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTGCGTTTGAAATACCAGGTTGcc < 3:493325/266‑1 (MQ=255) tggATACGGAGTTT‑‑ACTC‑C‑GCATGCGACATCCATGCCTGATGCGACTCTTGCCGCTTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTCCACAC‑CCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCTCCTTTTGCTTGGTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGTAATAACCGGTTCGGGAAATTGCAGAGCTGCGTTTGAAATACCAGGTTGCCTTGt < 2:321531/274‑1 (MQ=255) cGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAC‑CCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGTATAACCGGTTAGGGAAATTGCAGAGCTGCGTTTGAACTACCAGTTTGCCTTGt > 2:403906/1‑275 (MQ=255) ggATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAC‑CCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGCAGATCTGCGTTTAAAATACCCTGTTGCCTTGt > 4:553681/1‑274 (MQ=255) aaGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAC‑CCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTGCGTTTGAAATACCAgg < 2:553681/261‑1 (MQ=255) || TCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑A‑‑C‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAC‑CCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTGCGTTTGAAATACCAGGTTGCCTTGT > NC_000913/4296162‑4296638 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |