Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,809,681 T→A 9.2% V381D (GTT→GAT) ‡ waaA → 3‑deoxy‑D‑manno‑octulosonic‑acid transferase (KDO transferase)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,809,6810TA 9.2% 168.4 / 5.0 53V381D (GTT→GAT) ‡waaA3‑deoxy‑D‑manno‑octulosonic‑acid transferase (KDO transferase)
Reads supporting (aligned to +/- strand):  ref base T (19/29);  new base A (3/2);  total (22/31)
Fisher's exact test for biased strand distribution p-value = 6.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCATAATCCGCTGGAAGCTGCCGCACACGCTATTCCGGTATTGATGGGGCCGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAAACCAGGGCGCGCTACAGCGTCTGCTTCAACTGCTGGAACCTTACCTGCCACCGAAAACGCATTGAGGTTGTTAT  >  NC_000913/3809538‑3809826
                                                                                                                                               |                                                                                                                                                 
gCATAATCCGCTGGAAGCTGCCGCACACGCTATTCCGGTATTGATGGGGCCGGATAACTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGGAAGCCGGCTGGATACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCct                                                                                                                                              <  4:95886/149‑1 (MQ=255)
gCATAATCCGCTGGAAGCTGCCGCACACGCTATTCCGGTATTGATGGGGCCGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCct                                                                                                                                              <  4:16024/149‑1 (MQ=255)
gCATAATCCGCTGGAAGCTGCCGCACACGCTATTCCGGTATTGATGGGGCCGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCct                                                                                                                                              <  2:34288/149‑1 (MQ=255)
  aTAATCCGCTGGAAGCTGCCGCACACGCTATTCCGGTATTGATGGGGCCGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCctct                                                                                                                                            >  2:85266/1‑149 (MQ=255)
  aTAATCCGCTGGAAGCTGCCGCACACGCTATTCCGGTATTGATGGGGCCGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCctct                                                                                                                                            <  3:38081/149‑1 (MQ=255)
  aTAATCCGCTGGAAGCTGCCGCACACGCTATTCCGGTATTGATGGGGCCGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCctct                                                                                                                                            >  1:60586/1‑149 (MQ=255)
     aTCCGCTGGAAGCTGCCGCACACGCTATTCCGGTATTGATGGGGCCGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTa                                                                                                                                         <  1:25027/149‑1 (MQ=255)
         gCTGGAAGCTGCCGCACACGCTATTCCGGTATTGATGGGGCCGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGAAACCTCttttgcaa                                                                                                                                     <  1:32053/149‑6 (MQ=255)
                  tGCCGCACACGCTATTCCGGTATTGATGGGGCCGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGAAACCTCtttt                                                                                                                                         <  4:89985/136‑2 (MQ=255)
                  tGCCGCACACGCTATTCCGGTATTGATGGGGCCGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGAAACCTCtttt                                                                                                                                         >  3:89985/1‑135 (MQ=255)
                    ccGCACACGCTATTCCGGTATTGATGGGGCCGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGAt                                                                                                                          >  3:94984/1‑149 (MQ=255)
                        acacGCTATTCCGGTATTGATGGGGCCGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTAcc                                                                                                                      <  1:28856/149‑1 (MQ=255)
                          acGCTATTCCGGTATTGATGGGGCCGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGAAACCTCtttt                                                                                                                                         >  4:83019/1‑127 (MQ=255)
                               aTTCCGGTATTGATGGGGCCGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGttt                                                                                                               >  3:32823/1‑149 (MQ=255)
                                      tATTGATGGGGCCGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGc                                                                                                        >  4:17877/1‑149 (MQ=255)
                                              gggCCGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGc                                                                                                >  4:73792/1‑149 (MQ=255)
                                              gggCCGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGc                                                                                                >  1:82641/1‑149 (MQ=255)
                                                   gCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTg                                                                                           >  4:47044/1‑149 (MQ=255)
                                                   gCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTg                                                                                           >  2:39502/1‑149 (MQ=255)
                                                       aCTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGt                                                                                       >  1:26432/1‑149 (MQ=255)
                                                        cTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTa                                                                                      <  1:41124/149‑1 (MQ=255)
                                                                tttAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCa                                                                              >  1:95928/1‑149 (MQ=255)
                                                                tttAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCa                                                                              >  3:76742/1‑149 (MQ=255)
                                                                   aaaGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCaaaa                                                                           <  4:94984/149‑1 (MQ=255)
                                                                      gACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAAACCa                                                                        <  2:30330/149‑1 (MQ=255)
                                                                      gACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAAACCa                                                                        <  2:82641/149‑1 (MQ=255)
                                                                        cATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAAACCAgg                                                                      <  2:68133/149‑1 (MQ=255)
                                                                        cATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAAACCAgg                                                                      <  1:90474/149‑1 (MQ=255)
                                                                         aTTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAAACCAggg                                                                     <  3:84027/149‑1 (MQ=255)
                                                                          tttGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAAACCAGGgc                                                                    >  4:59497/1‑149 (MQ=255)
                                                                                cgcgGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAAACCAGGGCGCGCTa                                                              <  4:76742/149‑1 (MQ=255)
                                                                                 gcgGCAGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCCCTACGCTTGCAGATGAGGATTCCTCTTTCCTCACCGACGCCGATTACCGTAGTTTCTATGGCCGCCATGCCGTTGAAGTACTGTATCAAACCCAGGGCGCGATAc                                                             >  2:42265/1‑149 (MQ=255)
                                                                                       ggAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAAACCAGGGCGCGCTACAGCGTc                                                       <  2:60586/149‑1 (MQ=255)
                                                                                          gCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAAACCAGGGCGCGCTACAGCGTCTGc                                                    <  3:43930/149‑1 (MQ=255)
                                                                                             ggCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAAACCAGGGCGCGCTACAGCGTCTGCTTc                                                 <  3:79661/149‑1 (MQ=255)
                                                                                             ggCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAAACCAGGGCGCGCTACAGCGTCTGCTTc                                                 >  2:42495/1‑149 (MQ=255)
                                                                                              gCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAAACCAGGGCGCGCTACAGCGTCTGCTTCa                                                >  1:103008/1‑149 (MQ=255)
                                                                                               cAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAAACCAGGGCGCGCTACAGCGTCTGCTTCaa                                               <  3:24534/149‑1 (MQ=255)
                                                                                                aaGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAAACCAGGGCGCGCTACAGCGTCTGCTTCAAc                                              >  2:56810/1‑149 (MQ=255)
                                                                                                aaGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAAACCAGGGCGCGCTACAGCGTCTGCTTCAAc                                              <  4:32823/149‑1 (MQ=255)
                                                                                                        tGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAAACCAGGGCGCGCTACAGCGTCTGCtt                                                  <  2:63650/137‑1 (MQ=255)
                                                                                                        tGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAAACCAGGGCGCGCTACAGCGTCTGCtt                                                  >  1:63650/1‑137 (MQ=255)
                                                                                                         gATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAAACCAGGGCGCGCTACAGCGTCTGCTTCAACTGCTGGAAc                                     <  3:82669/149‑1 (MQ=255)
                                                                                                             accgttaccgATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAAACCAGGGCGCGCTACAGCGTCTGCTTCAACTGCTGGAACCTTa                                 <  3:84695/149‑1 (MQ=255)
                                                                                                              ccgttaccgATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAAACCAGGGCGCGCTACAGCGTCTGCTTCAACTGCTGGAACCTTAc                                <  1:39502/149‑1 (MQ=255)
                                                                                                                 ttaccgATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAAACCAGGGCGCGCTACAGCGTCTGCTTCAACTGCTGGAACCTTACCTg                             >  4:16782/1‑149 (MQ=255)
                                                                                                                      gATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAAACCAGGGCGCGCTACAGCGTCTGCTTCAACTGCTGGAACCTTACCTGCCAcc                        >  2:40889/1‑149 (MQ=255)
                                                                                                                            aCTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAAACCAGGGCGCGCTACAGCGTCTGCTTCAACTGCTGGAACCTTACCTGCCACCGAAAAc                  <  4:19792/149‑1 (MQ=255)
                                                                                                                               aCGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAAACCAGGGCGCGCTACAGCGTCTGCTTCAACTGCTGGAACCTTACCTGCCCCCGAAAACGCa               <  3:59178/149‑1 (MQ=255)
                                                                                                                                cGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGCCGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAAACCAGGGCGCGCTACAGCGTCTGCTTCAACTGCTGGAACCTTACCTGCCACCGAAAACGCAt              <  4:66672/149‑1 (MQ=255)
                                                                                                                                       aaaaGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAAACCAGGGCGCGCTACAGCGTCTGCTTCAACTGCTGGAACCTTACCTGCCACCGAAAACGCATTGAGgtt       <  2:1578/149‑1 (MQ=255)
                                                                                                                                          agagGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAAACCAGGGCGCGCTACAGCGTCTGCTTCAACTGCTGGAACCTTACCTGCCACCGAAAACGCATTGAGgttgtt    <  1:82657/149‑1 (MQ=255)
                                                                                                                                            agGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAAACCAGGGCGCGCTACAGCGTCTGCTTCAACTGCTGGAACCTTACCTGCCACCGAAAACGCATTGAGGTTGTTAt  <  2:26432/149‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                 
GCATAATCCGCTGGAAGCTGCCGCACACGCTATTCCGGTATTGATGGGGCCGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAAACCAGGGCGCGCTACAGCGTCTGCTTCAACTGCTGGAACCTTACCTGCCACCGAAAACGCATTGAGGTTGTTAT  >  NC_000913/3809538‑3809826

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑