| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,071,555 | G→A | 36.4% | H71Y (CAT→TAT) | rutD ← | putative reactive intermediate detoxifying aminoacrylate hydrolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,071,555 | 0 | G | A | 36.4% | 5.8 / 6.6 | 11 | H71Y (CAT→TAT) | rutD | putative reactive intermediate detoxifying aminoacrylate hydrolase |
| Reads supporting (aligned to +/- strand): ref base G (4/3); new base A (2/2); total (6/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.01e-01 | |||||||||||
ATTCGTAGCCAGCCGTTAACGCTGATCAGCACAGTTACCGACGCGGGATAATCCAGCGCCAGCTGCATTCCCACCAGCGCACCGAGCGCATGGCCGACCACTGCGTAATGCTCAATCCCTGCGGCTACCAGCGCCTGATGCAGTTCCGCTGCCATCTGGGCGATACTGTAATCTTCTGCCAGCGTGTCGGGATTATTGCCGGTGCCGCGCTGGTCGTAACAGACTACCTGATACTCCTGCTCCAGCACCGCCAGTTGCGGTAACCAG > NC_000913/1071416‑1071682 | aTTCGTAGCCAGCCGTTAACGCTGATCAGCACAGTTACCGACGCGGGATAATCCAGCGCCAGCTGCATTCCCACCAGCGCACCGAGCGCATGGCCGACCACTGCGTAATGCTCAATCCCTGCGGCTACCAGCGCCTGATGCAGTTCCGc < 3:32176/149‑1 (MQ=255) ttCGTAGCCAGCCGTTAACGCTGATCAGCACAGTTACCGACGCGGGATAATCCAGCGCCAGCTGCATTCCCACCAGCGCACCGAGCGCATGGCCGACCACTGCGTAATGCTCAATCCCTGCGGCTACCAGCGCCTGATACAGTTCCGCt > 2:12028/1‑149 (MQ=255) ttCGTAGCCAGCCGTTAACGCTGATCAGCACAGTTACCGACGCGGGATAATCCAGCGCCAGCTGCATTCCCACCAGCGCACCGAGCGCATGGCCGACCACTGCGTAATGCTCAATCCCTGCGGCTACCAGCGCCTGATACAGTTCCGCt > 2:59034/1‑149 (MQ=255) cTGATCAGCACAGTTACCGACGCGGGATAATCCAGCGCCAGCTGCATTCCCACCAGCGCACCGAGCGCATGGCCGACCACTGCGTAATGCTCAATCCCTGCGGCTACCAGCGCCTGATGCAGTTCCGCTGCCATCTGGGCGATACTGTa > 1:48879/1‑149 (MQ=255) ccGACGCGGGATAATCCAGCGCCAGCTGCATTCCCACCAGCGCACCGAGCGCATGGCCGACCACTGCGTAATGCTCAATCCCTGCGGCTACCAGCGCCTGATGCa < 1:12055/105‑1 (MQ=255) ccGACGCGGGATAATCCAGCGCCAGCTGCATTCCCACCAGCGCACCGAGCGCATGGCCGACCACTGCGTAATGCTCAATCCCTGCGGCTACCAGCGCCTGATGCa > 2:12055/1‑105 (MQ=255) gACGCGGGATAATCCAGCGCCAGCTGCATTCCCACCAGCGCACCGAGCGCATGGCCGACCACTGCGTAATGCTCAATCCCTGCGGCTACCAGCGCCTGATGCAGTTCCGCTGCCATCTGGGCGATACTGTAATCTTCTGCCAGCGTGTc < 2:71383/149‑1 (MQ=255) aTCCAGCGCCAGCTGCATTCCCACCAGCGCACCGAGCGCATGGCCGACCACTGCGTAATGCTCAATCCCTGCGGCTACCAGCGCCTGATACAGTTCCGCTGCCATCTGGGCGATACTGTAATCTTCTGCCAGCGTGTCGGGATTATTGc < 1:12028/149‑1 (MQ=255) aTCCAGCGCCAGCTGCATTCCCACCAGCGCACCGAGCGCATGGCCGACCACTGCGTAATGCTCAATCCCTGCGGCTACCAGCGCCTGATACAGTTCCGCTGCCATCTGGGCGATACTGTAATCTTCTGCCAGCGTGTCGGGATTATTGc < 1:59034/149‑1 (MQ=255) gTAATGCTCAATCCCTGCGGCTACCAGCGCCTGATGCAGTTCCGCTGCCATCTGGGCGATACTGTAATCTTCTGCCAGCGTGTCGGGATTATTGCCGGTGCCGCGCTGGTCGTAACAGACTACCTGATACTCCTGCTCCAGCACCGCCa > 3:20199/1‑149 (MQ=255) cTGCGGCTACCAGCGCCTGATGCAGTTCCGCTGCCATCTGGGCGATACTGTAATCTTCTGCCAGCGTGTCGGGATTATTGCCGGTGCCGCGCTGGTCGTAACAGACTACCTGATACTCCTGCTCCAGCACCGCCAGTTGCGGTAACCAg > 2:42180/1‑149 (MQ=255) | ATTCGTAGCCAGCCGTTAACGCTGATCAGCACAGTTACCGACGCGGGATAATCCAGCGCCAGCTGCATTCCCACCAGCGCACCGAGCGCATGGCCGACCACTGCGTAATGCTCAATCCCTGCGGCTACCAGCGCCTGATGCAGTTCCGCTGCCATCTGGGCGATACTGTAATCTTCTGCCAGCGTGTCGGGATTATTGCCGGTGCCGCGCTGGTCGTAACAGACTACCTGATACTCCTGCTCCAGCACCGCCAGTTGCGGTAACCAG > NC_000913/1071416‑1071682 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |