Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,795,227 A→T 44.5% intergenic (‑75/+26) btuC ← / ← ihfA vitamin B12 ABC transporter permease/integration host factor (IHF), DNA‑binding protein, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,795,2270AT44.5% ‑4.4 / 6.3 9intergenic (‑75/+26)btuC/ihfAvitamin B12 ABC transporter permease/integration host factor (IHF), DNA‑binding protein, alpha subunit
Reads supporting (aligned to +/- strand):  ref base A (2/3);  new base T (2/2);  total (4/5)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.29e-01

CAGCATCAAAACTGACAGGCATAATAACCAGCGAATATTTTGTCGCTGTTGTTGGCGGGCAAGTGTCAGCATGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGAAGGTCACCACGCGCCGTGCTGTAATGGGAATATCCTCGCCCGTTTTCGGGTTACGT  >  NC_000913/1795081‑1795367
                                                                                                                                                  |                                                                                                                                            
cagcaTCAAAACTGACAGGCATAATAACCAGCGAATATTTTGTCGCTGTTGTTGGCGGGCAAGTGTCAGCATGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCaga                                                                                                                                            <  2:37649/149‑1 (MQ=255)
    aTCAAAACTGACAGGCATAATAACCAGCGAATATTTTGTCGCTGTTGTTGGCGGGCAAGTGTCAGCATGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCgg                                                                                                                                        <  1:35973/149‑1 (MQ=255)
           cTGACAGGCATAATAACCAGCGAATATTTTGTCGCTGTTGTTGGCGGGCAAGTGTCAGCATGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCTCTGCGGCCttttt                                                                                                                                 <  3:98674/149‑1 (MQ=255)
                                            gCTGTTGTTGGCGGGCAAGTGTCAGCATGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGGAGc                                                                                                >  1:91458/1‑149 (MQ=255)
                                                                 tCAGCATGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTaa                                                                           <  2:102697/149‑1 (MQ=255)
                                                                                   tgctgAAGTGTCATCGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGTGTCTGaa                                                         >  3:7273/1‑149 (MQ=255)
                                                                                                    gTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTATATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGAAGGTCACCACGCGCCGTg                                        <  4:7273/149‑1 (MQ=255)
                                                                                                                                      taaaaaGGCCGCTCTGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCgg                                                                <  1:86710/90‑1 (MQ=39)
                                                                                                                                      taaaaaGGCCGCTCTGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCgg                                                                >  2:86710/2‑91 (MQ=39)
                                                                                                                                          aaGGCCGCTCTGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGAAGGTCACCACGCGCCGTGCTGTAATGGGAATATCCTCGCCCGTTTTCGGGTTACGt  >  1:60281/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                            
CAGCATCAAAACTGACAGGCATAATAACCAGCGAATATTTTGTCGCTGTTGTTGGCGGGCAAGTGTCAGCATGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGAAGGTCACCACGCGCCGTGCTGTAATGGGAATATCCTCGCCCGTTTTCGGGTTACGT  >  NC_000913/1795081‑1795367

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: