Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,963,299 Δ1 bp 9.5% coding (931/1485 nt) gpp ← guanosine pentaphosphatase/exopolyphosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,963,2990G. 9.5% 307.4 / 27.7 95coding (931/1485 nt)gppguanosine pentaphosphatase/exopolyphosphatase
Reads supporting (aligned to +/- strand):  ref base G (50/36);  new base . (3/6);  total (53/42)
Fisher's exact test for biased strand distribution p-value = 1.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.18e-01

AGGCGCTGATGAGCAAATCGCGGCTTATTGCTTCAAGATGCCATTCGTTTTCCACCTGATCGAAGAAGTTAGCCGCAACTTTGGCTACGCGCTGTGCCTGATCAATATCGATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCA  >  NC_000913/3963155‑3963447
                                                                                                                                                 |                                                                                                                                                    
aGGCGCTGATGAGCAAATCGCGGCTTATTGCTTCAAGATGCCATTCGTTTTCCACCTGATCGAAGAAGTTAGCCGCAACTTTGGCTACGCGCTGTGCCTGATCAATATCGATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTg                                                                                                                                                  >  4:670570/1‑149 (MQ=255)
 ggCGCTGATGAGCAAATCGCGGCTTATTGCTTCAAGATGCCATTCGTTTTCCACCTGATCGAAGAAGTTAGCCGCAACTTTGGCTACGCGCTGTGCCTGATCAATATCGATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGc                                                                                                                                                 <  4:550194/149‑1 (MQ=255)
  gcgcTGATGAGCAAATCGCGGCTTATTGCTTCAAGATGCCATTTGTTTTCCACCTGATCGAATAAGTTAGCCGCAACTCTGGCTACGCGCTGTGCCTGATCAATATCAATCATAAAGTGGCGTTGAATATTACGCAGCGTACGGCTGCg                                                                                                                                                >  3:773157/1‑149 (MQ=255)
     cTGATGAGCAAATCGCGGCTTATTGCTTCAAGATGCCATTCGTTTTCCGCCTGATCGAAGAAGTTAGCCGCAACTTTGGCTACGCGCTGTGCCTGATCAATATCGATCATAAAGCGGCGTTGAATATTACGCAGCCGTACGCTGCgtaa                                                                                                                                             <  4:209816/149‑4 (MQ=255)
      tgatgaGCAAATCGCGGCTTATTGCTTCAAGATGCCATTCGTTTTCCACCTGATCGAAGAAGTTAGCCGCAACTTTGGCTACGCGCTGTGCCTGATCAATATCGATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAata                                                                                                                                            >  4:818068/1‑149 (MQ=255)
      tgatgaGCAAATCGCGGCTTATTGCTTCAAGATGCCATTCGTTTTCCACCTGATCGAAGAAGTTAGCCGCAACTTTGGCTACGCGCTGTGCCTGATCAATATCGATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAata                                                                                                                                            >  2:662320/1‑149 (MQ=255)
       gatgaGCAAATCGCGGCTTATTGCTTCAAGATGCCATTCGTTTTCCACCTGATCGAAGAAGTTAGCCGCAACTTTGGCTACGCGCTGTGCCTGATCAATATCGATCATAAAGCGGCGTTGAATATTACGCAGCCGTACGCTGCgtaata                                                                                                                                           <  4:20004/149‑6 (MQ=255)
       gatgaGCAAATCGCGGCTTATTGCTTCAAGATGCCATTCGTTTTCCACCTGATCGAAGAAGTTAGCCGCAACTTTGGCTACGCGCTGTGCCTGATCAATATCGATCATAAAGCGGCGTTGAATATTACGCAGCCGTACGCTGCgtaata                                                                                                                                           <  1:499690/149‑6 (MQ=255)
        atgaGCAAATCGCGGCTTATTGCTTCAAGATGCCATTCGTTTTCCACCTGATCGAAGAAGTTAGCCGCAACTTTGGCTACGCGCTTTGCCTGATCAATATCGATCATAAAGCGGCGTTGAATATTACGCAGCCGTACGCTGCgtaata                                                                                                                                           <  4:283026/148‑6 (MQ=255)
        atgaGCAAATCGCGGCTTATTGCTTCAAGATGCCATTCGTTTTCCACCTGATCGAAGAAGTTAGCCGCAACTTTGGCTACGCGCTGTGCCTGATCAATATCGATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATc                                                                                                                                          <  1:545042/149‑1 (MQ=255)
         tgaGCAAATCGCGGCTTATTGCTTCAAGATGCCATTCGTTTTCCACCTGATCGAAGAAGTTAGCCGCAACTTTGGCTACGCGCTGTGCCTGATCAATATCGATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATcc                                                                                                                                         <  2:933920/149‑1 (MQ=255)
          gaGCAAATCGCGGCTTATTGCTTCAAGATGCCATTCGTTTTCCACCTGATCGAAGAAGTTAGCCGCAACTTTGGCTACGCGCTGTGCCTGATCAATATCGATCATAAAGCGGCGTTGAATATTACGAAGCGTACGGCTGCGAATATCCt                                                                                                                                        >  4:761812/1‑149 (MQ=255)
           aGCAAATCGCGGCTTATTGCTTCAAGATGCCATTCGTTTTCCACCTGATCGAAGAAGTTAGCCGCAACTTTGGCTACGCGCTGTGCCTGATCAATATCGATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTg                                                                                                                                       >  1:150126/1‑149 (MQ=255)
            gCAAATCGCGGCTTATTGCTTCAAGATGCCATTCGTTTTCCACCTGATCGAAGAAGTTAGCCGCAACTTTGGCTACGCGCTGTGCCTGATCAATATCGATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGc                                                                                                                                      <  1:608485/149‑1 (MQ=255)
             cAAATCGCGGCTTATTGCTTCAAGATGCCATTCGTTTTCCACCTGATCGAAGAAGTTAGCCGCAACTTTGGCTACGCGCTGTGCCTGATCAATATCGATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCt                                                                                                                                     <  1:69057/149‑1 (MQ=255)
              aaaTCGCGGCTTATTGCTTCAAGATGCCATTCGTTTTCCACCTGATCGAAGAAGTTAGCCGCAACTTTGGCTACGCGCTGTGCCTGATCAATATCGATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTc                                                                                                                                    >  3:894428/1‑149 (MQ=255)
                      gCTTATTGCTTCAAGATGCCATTCGTTTTCCACCTGATCGAAGAAGTTAGCCGCAACTTTGGCTACGCGCTGTGCCTGATCAATATCGATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCa                                                                                                                            >  4:26333/1‑149 (MQ=255)
                          aTTGCTTCAAGATGCCATTCGTTTTCCACCTGATCGAAGAAGTTAGCCGCAACTTTGGCTACGCGCTGTGCCTGATCAGTATCGATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATg                                                                                                                        >  4:664669/1‑149 (MQ=255)
                           ttGCTTCAAGATGCCATTCGTTTTCCACCTGATCGAAGAAGTTAGCCGCAACTTTGGCTACGCGCTGTGCCTGATCAATATCGATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGc                                                                                                                       <  1:422466/149‑1 (MQ=255)
                             gCTTCAAGATGCCATTCGTTTTCCACCTGATCGAAGAAGTTAGCCGCAACTTTGGCTACGCGCTGTGCCTGATCAATATCGATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCaa                                                                                                                     <  2:343532/149‑1 (MQ=255)
                              cTTCAAGATGCCATTCGTTTTCCACCTGATCGAAGAAGTTAGCCGCAACTTTGGCTACGCGCTGTGCCTGATCAATATCGATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAAc                                                                                                                    >  1:356805/1‑149 (MQ=255)
                               ttCAAGATGCCGTTCGTTTTCCACCTGATCGAAGAAGTTAGCCGCAACTTTGGCTACGCGCTGTGCCTGATCAATATCGATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACa                                                                                                                   >  3:541322/1‑149 (MQ=255)
                                  aaGATGCCATTCGTTTTCCACCTGATCGAAGAAGTTAGCCGCAACTTTGGCTACGCGCTGTGCCTGATCAATATCGATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGc                                                                                                                >  3:735974/1‑149 (MQ=255)
                                      tGCCATTCGTTTTCCACCTGATCGAAGAAGTTAGCCGCAACTTTGGCTACGCGCTGTGCCTGATCAATATCGATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTa                                                                                                            <  1:877527/149‑1 (MQ=255)
                                       gCCATTCGTTTTCCACCTGATCGAAGAAGTTAGCCGCAACTTTGGCTACGCGCTGTGCCTGATCAATATCGATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAg                                                                                                           <  1:454060/149‑1 (MQ=255)
                                        ccATTCGTTTTCCACCTGATCGAAGAAGTTAGCCGCAACTTTGGCTACGCGCTGTGCCTGATCAATATCGATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGa                                                                                                          >  4:615922/1‑149 (MQ=255)
                                          aTTCGTTTTCCACCTGATCGACGACGTTAATCGCAGCTTTGGCTACGCGCTGTGCCTGATCAATATCGATCATAAAGCGCCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCGCGACGGCCAGATGCAACATGCCGTAGAcc                                                                                                        >  4:775211/1‑149 (MQ=255)
                                          aTTCGTTTTCCACCTGATCGAAGAAGTTAGCCGCAACTTTGGCTACGCGCTGTGCCTGATCAATATCGATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGAcc                                                                                                        >  2:816298/1‑149 (MQ=255)
                                          aTTCGTTTTCCACCTGATCGAAGAAGTTAGCCGCAACTTTGGCTACGCGCTGTGCCTGATCAATATCGATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGAcc                                                                                                        >  1:422142/1‑149 (MQ=255)
                                               ttttCCACCTGATCGTAGAAGTTAGCCGCGCCTTTGGCTACGCGCTGTGCCTGATCAATATCGATCATTAAGCGACGTTGAATATGACGGAGCGTACGGCTGCGAATATCCTGCTCGACGGCGCGATGCAACATGCCGTAGACCAGGcc                                                                                                   >  4:775093/1‑149 (MQ=255)
                                               ttttCCACCTGATCGAAGAAGTTAGCCGCAACTTTGGCTACGCGCTGTGCCTGATCAATATCGATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGTcc                                                                                                   >  2:649944/1‑149 (MQ=255)
                                                      ccTGATCGAAGAAGTTAGCCGCAACTTTGGCTACGCGCTGTGCCTGATCAATATCGATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGc                                                                                            >  4:653069/1‑149 (MQ=255)
                                                      ccTGATCGAAGAAGTTAGCCGCAACTTTGGCTACGCGCTGTGCCTGATCAATATCGATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATAGCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGc                                                                                            <  1:873470/149‑1 (MQ=255)
                                                       cTGATCGAAGAAGTTAGCCGCAACTTTGGCTACGCGCTGTGCCTGATCAATATCGATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCa                                                                                           >  4:474944/1‑149 (MQ=255)
                                                         gATCGAAGAAGTTAGCCGCAACTTTGGCTACGCGCTGTGCCTGATCAATATCGAGCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAgc                                                                                         <  1:662320/149‑1 (MQ=255)
                                                             gaagaaGTTAGCCGCAACTTTGGCTACGCGCTGTGCCTGATCAATATCGATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCAc                                                                                     >  3:432319/1‑149 (MQ=255)
                                                                   gTTAGCCGCAACTTTGGCTACGCGCTGTGCCTGATCAATATCGATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCAccgcccg                                                                               >  2:880991/1‑149 (MQ=255)
                                                                    ttAGCCGCAACTTTGGCTACGCGCTGTGCCTGATCAATATCGATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGc                                                                                                    >  3:593639/1‑127 (MQ=255)
                                                                    ttAGCCGCAACTTTGGCTACGCGCTGTGCCTGATCAATATCGATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGc                                                                                                    <  4:593639/127‑1 (MQ=255)
                                                                    ttAGCCGCAACTTTGGCTACGCGCTGTGCCTGATCAATATCGATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCACAccgcccgc                                                                              >  4:791130/1‑149 (MQ=255)
                                                                        ccGCAACTTTGGCTACGCGCTGTGCCTGATCAATATCGATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAgg                                                                          <  2:356805/149‑1 (MQ=255)
                                                                         cGCAACCTTGGCTACGCGCTGTGCCTGATCAATATCGATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAAACCAGGCCTTCACGCAGCGCACCGCCCGCCAggg                                                                         <  3:615922/149‑1 (MQ=255)
                                                                          gCAACTTTGGCTACGCGCTGTGCCTGATCAATATCGATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGt                                                                        >  3:290655/1‑149 (MQ=255)
                                                                          gCAACTTTGGCTACGCGCTGTGCCTGATCAATATCGATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGt                                                                        >  3:35476/1‑149 (MQ=255)
                                                                            aaCTTTGGCTACGCGCTGTGCCTGATCAATATCGATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCa                                                                      <  4:735974/149‑1 (MQ=255)
                                                                              cTTTGGCTACGCGCTGTGCCTGATCAATATCGATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATa                                                                    <  4:894428/149‑1 (MQ=255)
                                                                               tttGGCTACGCGCTGTGCCTGATCAATATCGATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGa                                                                                                          >  4:129058/1‑110 (MQ=255)
                                                                               tttGGCTACGCGCTGTGCCTGATCAATATCGATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGa                                                                                                          <  3:129058/110‑1 (MQ=255)
                                                                               tttGGCTACGCGCTGTGCCTGATCAATATCGATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATac                                                                   >  2:220249/1‑149 (MQ=255)
                                                                                  ggCTACGCGCTGTGCCTGATCAATATCGATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACt                                                                >  4:787911/1‑149 (MQ=255)
                                                                                     tACGCGCTGTGCCTGATCAATATCGATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGaa                                                             <  1:576469/149‑1 (MQ=255)
                                                                                      aCGCGCTGTGCCTGATCAATATCGATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAat                                                            >  3:409778/1‑149 (MQ=255)
                                                                                         cgcTGTGCCTGATCAATATCGATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATAtt                                                         <  1:816298/149‑1 (MQ=255)
                                                                                          gcTGTGCCTGATCAATATCGATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTc                                                        <  1:220249/149‑1 (MQ=255)
                                                                                           cTGTGCCTGATCAATATCGATCATAAAGCGGCGTTGAATATTACTCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCa                                                       <  3:791130/149‑1 (MQ=255)
                                                                                           cTGTGCCTGATCAATATCGATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAata                                                                                                                                            >  1:407387/1‑64 (MQ=255)
                                                                                           cTGTGCCTGATCAATATCGATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAata                                                                                                                                            <  2:407387/64‑1 (MQ=255)
                                                                                            tgtgCCTGATCAATATCGATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAg                                                      <  2:150126/149‑1 (MQ=255)
                                                                                              tgCCTGATCAATATCGATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGtt                                                    <  4:35476/149‑1 (MQ=255)
                                                                                                ccTGATCAATATCGATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCg                                                  >  1:725726/1‑149 (MQ=255)
                                                                                                   gATCAATATCGATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTa                                               >  2:420575/1‑149 (MQ=255)
                                                                                                    aTCAATATCGATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTaa                                              >  3:696471/1‑149 (MQ=255)
                                                                                                    aTCAATATCGATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTaa                                              >  1:632749/1‑149 (MQ=255)
                                                                                                          atCGATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATgg                                        >  1:387597/1‑149 (MQ=255)
                                                                                                             gATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGa                                     >  4:848648/1‑149 (MQ=255)
                                                                                                              aTCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGAt                                    >  2:685887/1‑149 (MQ=255)
                                                                                                               tCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCa                                                       >  4:70220/1‑129 (MQ=255)
                                                                                                               tCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATc                                   >  2:46947/1‑149 (MQ=255)
                                                                                                               tCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCACACACTGAATATTCa                                                       <  3:70220/129‑1 (MQ=255)
                                                                                                                 aTAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGc                                       <  2:613990/143‑1 (MQ=255)
                                                                                                                 aTAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGc                                       >  1:613990/1‑143 (MQ=255)
                                                                                                                 aTAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAg                                 >  2:580939/1‑149 (MQ=255)
                                                                                                                 aTAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAg                                 >  3:511448/1‑149 (MQ=255)
                                                                                                                  tAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAgg                                >  2:213134/1‑149 (MQ=255)
                                                                                                                     aGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATc                             <  1:873745/149‑1 (MQ=255)
                                                                                                                     aGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATc                             >  3:555442/1‑149 (MQ=255)
                                                                                                                        ggcgTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCCTGATCGcc                          <  4:541322/149‑1 (MQ=255)
                                                                                                                         gcgTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCa                         <  1:567537/149‑1 (MQ=255)
                                                                                                                          cgTTGAATCTTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCATCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCTGTAAAAATGGCGATCCTGATCGCCat                        <  3:775211/149‑2 (MQ=255)
                                                                                                                           gTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCatt                       <  1:46947/149‑3 (MQ=255)
                                                                                                                            ttGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCCTGATCGCCatt                       <  1:851535/148‑3 (MQ=255)
                                                                                                                            ttGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCCTGATCGCCatt                       >  2:851535/1‑146 (MQ=255)
                                                                                                                            ttGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGAc                      >  4:499824/1‑149 (MQ=255)
                                                                                                                            ttGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGAc                      <  4:511448/149‑1 (MQ=255)
                                                                                                                                  tattcGCAGCCGTACGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATg                                         >  3:267516/5‑124 (MQ=255)
                                                                                                                                  tattcGCAGCCGTACGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATg                                         <  4:267516/120‑1 (MQ=255)
                                                                                                                                   ttACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCgg               >  3:273405/1‑149 (MQ=255)
                                                                                                                                   ttACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCgg               >  4:47480/1‑149 (MQ=255)
                                                                                                                                    tACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCggg              <  1:580939/149‑1 (MQ=255)
                                                                                                                                     tcGCAGCCGTACGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGc                                       >  1:27688/2‑123 (MQ=255)
                                                                                                                                     tcGCAGCCGTACGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGc                                       <  2:27688/122‑1 (MQ=255)
                                                                                                                                     tcGCAGCCGTACGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGa             >  3:263205/2‑149 (MQ=255)
                                                                                                                                      cGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAATTGGCGATCAGGATCGCCAGACCACTCGGGaa            <  2:4300/149‑1 (MQ=255)
                                                                                                                                      cGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGaa            >  1:887152/1‑149 (MQ=255)
                                                                                                                                        cAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAaca          <  1:420575/149‑1 (MQ=255)
                                                                                                                                                 ggCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCa  >  4:633532/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
AGGCGCTGATGAGCAAATCGCGGCTTATTGCTTCAAGATGCCATTCGTTTTCCACCTGATCGAAGAAGTTAGCCGCAACTTTGGCTACGCGCTGTGCCTGATCAATATCGATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCA  >  NC_000913/3963155‑3963447

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: