Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,868,865:1 +G 7.6% intergenic (+23/‑90) yeaG → / → yeaH protein kinase, endogenous substrate unidentified; autokinase/UPF0229 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,868,8651.G 7.6% 491.4 / 37.9 118intergenic (+23/‑90)yeaG/yeaHprotein kinase, endogenous substrate unidentified; autokinase/UPF0229 family protein
Reads supporting (aligned to +/- strand):  ref base . (54/55);  new base G (5/4);  total (59/59)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.02e-01

Only 100 of 122 total aligned reads displayed.

CAAAACGTCAACCGACGAGCAGAAGAAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCG  >  NC_000913/1868716‑1869010
                                                                                                                                                      |                                                                                                                                                 
cAAAACGTCAACCGACGAGCAGAAGAAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTAACATGt                                                                                                                                                    >  3:404614/1‑149 (MQ=255)
  aaaCGTCAACCGACGAGCAGAAGAAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGTTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGtt                                                                                                                                                  >  2:667763/1‑149 (MQ=255)
  aaaCGTCAACCGACGAGCAGAAGAAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGtt                                                                                                                                                  >  3:787922/1‑149 (MQ=255)
      gTCAACCGACGAGCAGAAGAAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCg                                                                                                                                              >  3:8301/1‑149 (MQ=255)
         aaCCGACGAGCAGAAGAAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGc                                                                                                                                           >  3:219124/1‑149 (MQ=255)
          aCCGACGAGCAGAAGAAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCgg                                                                                                                                             >  2:589029/1‑146 (MQ=255)
          aCCGACGAGCAGAAGAAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCgg                                                                                                                                             <  1:589029/146‑1 (MQ=255)
             gacgaGCAGAAGAAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTAc                                                                                                                                       >  3:279563/1‑149 (MQ=255)
                  gCAGAAGAAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGcc                                                                                                                                          >  3:283872/1‑141 (MQ=255)
                    agaagaAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACa                                                                                                                                <  1:110609/149‑1 (MQ=255)
                     gaagaaACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAg                                                                                                                               >  2:933032/1‑149 (MQ=255)
                      aagaaACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGc                                                                                                                              <  4:8301/149‑1 (MQ=255)
                            cacGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCg                                                                                                                        >  4:261760/1‑149 (MQ=255)
                             acgacgACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGt                                                                                                                       <  4:924478/149‑1 (MQ=255)
                              cgacgaCTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTg                                                                                                                      <  3:475850/149‑1 (MQ=255)
                              cgacgaCTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTg                                                                                                                      >  1:81586/1‑149 (MQ=255)
                              cgacgaCTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTg                                                                                                                      <  3:194683/149‑1 (MQ=255)
                                    cTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTAACATGTGTACCGGGccag                                                                                                                                        <  1:317176/125‑3 (MQ=255)
                                     tttGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTga                                                                                                               <  4:279563/149‑1 (MQ=255)
                                     tttGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTga                                                                                                               <  1:463076/149‑1 (MQ=255)
                                         tCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAAAAg                                                                                                           <  4:787922/149‑1 (MQ=255)
                                          cGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGc                                                                                                          <  1:854267/149‑1 (MQ=255)
                                           gACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCg                                                                                                         >  2:575907/1‑149 (MQ=255)
                                             ccGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTTCAAATGGTATTTGCGCGTACGTAAATCGTCTTAACACCCCTGGCCCGGTACACATGTTACCGGGCCTCCAACGTCAGCGAACCGTGGGCCTGAGATGCGGc                                                                                                       <  2:145665/149‑1 (MQ=255)
                                             ccGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCt                                                                                                                 <  1:602772/139‑1 (MQ=255)
                                             ccGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCt                                                                                                                 >  2:602772/1‑139 (MQ=255)
                                             ccGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGc                                                                                                       >  2:866272/1‑149 (MQ=255)
                                                tATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAac                                                                                                    >  2:266890/1‑149 (MQ=255)
                                                  tgatgGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAacac                                                                                                  >  3:463674/1‑149 (MQ=255)
                                                    atgGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGAc                                                                                                                                 >  3:313944/1‑116 (MQ=255)
                                                    atgGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGAc                                                                                                                                 <  4:313944/116‑1 (MQ=255)
                                                    atgGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAg                                                                                                >  1:638587/1‑149 (MQ=255)
                                                     tgGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAgg                                                                                               >  3:611991/1‑149 (MQ=255)
                                                     tgGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAgg                                                                                               >  2:759436/1‑149 (MQ=255)
                                                      ggAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGc                                                                                              <  3:530077/149‑1 (MQ=255)
                                                      ggAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGc                                                                                              >  1:194384/1‑149 (MQ=255)
                                                      ggAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGc                                                                                              <  3:706293/149‑1 (MQ=255)
                                                        aaaaaGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGt                                                                                            >  4:114992/1‑149 (MQ=255)
                                                            aGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGcc                                                                                                                                          >  1:705383/1‑99 (MQ=255)
                                                             ggCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCAt                                                                                       >  3:380582/1‑149 (MQ=255)
                                                             ggCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCAt                                                                                       >  2:300819/1‑149 (MQ=255)
                                                                 acacCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAg                                                                                   >  1:27850/1‑149 (MQ=255)
                                                                   acCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGtt                                                                                 <  3:472196/149‑1 (MQ=255)
                                                                   acCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGtt                                                                                 >  1:252501/1‑149 (MQ=255)
                                                                     ccGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGc                                                                                                                              >  2:741159/1‑102 (MQ=255)
                                                                       gTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCa                                                                             >  3:738786/1‑149 (MQ=255)
                                                                       gTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCa                                                                             >  1:119805/1‑149 (MQ=255)
                                                                        tAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTCGGCaa                                                                            <  1:266890/149‑1 (MQ=255)
                                                                               gTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATgtagt                                                                     >  4:831624/1‑149 (MQ=255)
                                                                               gTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATgtagt                                                                     >  3:38854/1‑149 (MQ=255)
                                                                                tGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAAGCGTGGGCCTGAGAAGCGGCAACACAGGCGTCGCATACAGTTGGCCAATgtagtg                                                                    >  1:163022/1‑148 (MQ=255)
                                                                                    tttACTGTGCGAATGGTATTCGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACggg                                                                >  1:560440/1‑149 (MQ=255)
                                                                                     ttACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACgggg                                                               <  2:638587/149‑1 (MQ=255)
                                                                                      tACTGTGCGAATGGTATTTGCGCGTGCGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACggggg                                                              <  1:493073/149‑1 (MQ=255)
                                                                                      tACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACggggg                                                              <  4:463674/149‑1 (MQ=255)
                                                                                        cTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGc                                                            <  1:933032/149‑1 (MQ=255)
                                                                                          gtgCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCat                                                          <  1:9318/149‑1 (MQ=255)
                                                                                              gAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGa                                                      <  3:573188/149‑1 (MQ=255)
                                                                                               aaTGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGAc                                                     <  2:214060/149‑1 (MQ=255)
                                                                                                aTGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGAcc                                                    >  3:424976/1‑149 (MQ=255)
                                                                                                 tGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATgtag                                                                      >  4:181938/1‑130 (MQ=255)
                                                                                                 tGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATgtag                                                                      <  3:181938/130‑1 (MQ=255)
                                                                                                 tGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCt                                                   >  4:7057/1‑149 (MQ=255)
                                                                                                     aTTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGtt                                               <  4:738786/149‑1 (MQ=255)
                                                                                                     aTTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGtt                                               <  2:532649/149‑1 (MQ=255)
                                                                                                      tttGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGttt                                              <  3:340874/149‑1 (MQ=255)
                                                                                                      tttGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGttt                                              >  3:361576/1‑149 (MQ=255)
                                                                                                       ttGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTa                                             <  2:81586/149‑1 (MQ=255)
                                                                                                       ttGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTa                                             <  4:424976/149‑1 (MQ=255)
                                                                                                        tGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTAt                                            <  4:361576/149‑1 (MQ=255)
                                                                                                        tGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTAt                                            <  3:114992/149‑1 (MQ=255)
                                                                                                        tGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATAACCTGGTTTAt                                            <  1:792594/149‑1 (MQ=255)
                                                                                                           gcgTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGa                                         <  3:619519/149‑1 (MQ=255)
                                                                                                                cgtaAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGc                                    <  3:831624/149‑1 (MQ=255)
                                                                                                                cgtaAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGc                                    <  4:443282/149‑1 (MQ=255)
                                                                                                                           ttAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGc                         >  4:375109/1‑149 (MQ=255)
                                                                                                                             aacaacCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGa                                         >  2:807041/1‑131 (MQ=255)
                                                                                                                             aacaacCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGa                                         <  1:807041/131‑1 (MQ=255)
                                                                                                                              acaacCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCaaa                      >  4:915/1‑149 (MQ=255)
                                                                                                                                aacCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCaaaaa                    >  1:84538/1‑149 (MQ=255)
                                                                                                                                aacCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCaaaaa                    <  3:24468/149‑1 (MQ=255)
                                                                                                                                aacCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCaaaaa                    >  4:24468/1‑149 (MQ=255)
                                                                                                                                aacCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCaaaaa                    >  4:503075/1‑149 (MQ=255)
                                                                                                                                aacCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAAAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCaaaaa                    >  4:400251/1‑149 (MQ=255)
                                                                                                                                 acCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAAc                   <  1:866272/149‑1 (MQ=255)
                                                                                                                                     tGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACaaaa               <  3:145776/149‑1 (MQ=255)
                                                                                                                                      ggCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAAc                            >  2:522337/1‑135 (MQ=255)
                                                                                                                                      ggCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAAc                            <  1:522337/135‑1 (MQ=255)
                                                                                                                                        cccGGTAACATGTGTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACgg                                                                 <  2:77186/96‑1 (MQ=255)
                                                                                                                                        cccGGTAACATGTGTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACgg                                                                 >  1:77186/1‑96 (MQ=255)
                                                                                                                                        cccGGTAACATGTGTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCa            >  3:168183/1‑149 (MQ=255)
                                                                                                                                         ccGGTAACATGTGTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCAt           >  4:378055/1‑149 (MQ=255)
                                                                                                                                         ccGGTAACATGTGTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCAt           >  1:372097/1‑149 (MQ=255)
                                                                                                                                          cGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATg          <  1:154712/149‑1 (MQ=255)
                                                                                                                                           ggTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATgg         <  4:611991/149‑1 (MQ=255)
                                                                                                                                                acaTGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAAt    >  1:485533/1‑149 (MQ=255)
                                                                                                                                                acaTGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAAt    <  2:57649/149‑1 (MQ=255)
                                                                                                                                                  aTGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCg  >  2:359522/1‑149 (MQ=255)
                                                                                                                                                   tGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTAt                                            >  4:611131/1‑106 (MQ=255)
                                                                                                                                                   tGTTACCGGGCCTACAACGACACCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTAt                                            <  3:611131/106‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                 
CAAAACGTCAACCGACGAGCAGAAGAAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCG  >  NC_000913/1868716‑1869010

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: