| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,622,348:1 | +A | 6.0% | coding (115/639 nt) | purN → | phosphoribosylglycinamide formyltransferase 1 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,622,348 | 1 | . | A | 6.0% | 421.8 / 21.8 | 100 | coding (115/639 nt) | purN | phosphoribosylglycinamide formyltransferase 1 |
| Reads supporting (aligned to +/- strand): ref base . (48/46); new base A (3/3); total (51/49) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.06e-01 | |||||||||||
Only 100 of 103 total aligned reads displayed.
TCTGATTCCGAACAACGCGTGGTTATCGAATAATGAATATTGTGGTGCTTATTTCCGGCAACGGAAGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCATGAAATCGACATGTACGCACCCGATGTGGTCGTGCTGGCTGGTTT > NC_000913/2622202‑2622496 | tctGATTCCGAACAACGCGTGGTTATCGAATAATGAATATTGTGGTGCTTATTTCCGGCAACGGAAGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑Ac > 3:469026/1‑149 (MQ=255) gATTCCGAACAACGCGTGGTTATCGAATAATGAATATTGTGGTGCTTATTTCCGGCAACGGAAGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑Acgcg > 3:360345/1‑149 (MQ=255) aTTCCGAACAACGCGTGGTTATCGAATAATGAATATTGTGGTGCTTATTTCCGGCAACGGAAGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGt < 4:121291/149‑1 (MQ=255) ttCCGAACAACGCGTGGTTATCGAATAATGAATATTGTGGTGCTTATTTCCGGCAACGGAAGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGtt > 4:765895/1‑149 (MQ=255) tCCGAACAACGCGTGGTTATCGACTAATAAATATTGTGGTGCTTATTACCGGCAACGGAAGTAATTTACAGGCAAGTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTc < 3:781655/149‑1 (MQ=255) tCCGAACAACGCGTGGTTATCGAATAATGAATATTGTGGTGCTTATTTCCGGCAACGGAAGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTc < 1:833321/149‑1 (MQ=255) tCCGAACAACGCGTGGTTATCGAATAATGAATATTGTGGTGCTTATTTCCGGCAACGGAAGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTc < 2:229324/149‑1 (MQ=255) tCCGAACAACGCGTGGTTATCGAATAATGAATATTGTGGTGCTTATTTCCGGCAACGGAAGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTc < 3:248522/149‑1 (MQ=255) cGAACAACGCGTGGTTATCGAATAATGAATATTGTGGTGCTTATTTCCGGCAACGGAAGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCgg < 3:461262/149‑1 (MQ=255) cGAACAACGCGTGGTTATCGAATAATGAATATTGTGGTGCTTATTTCCGGCAACGGAAGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCgg < 1:780543/149‑1 (MQ=255) gAACAACGCGTGGTTATCGAATAATGAATATTGTGGTGCTTATTTCCGGCAACGGAAGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGc > 4:241356/1‑149 (MQ=255) gAACAACGCGTGGTTATCGAATAATGAATATTGTGGTGCTTATTTCCGGCAACGGAAGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGc < 4:301379/149‑1 (MQ=255) gAACAACGCGTGGTTATCGAATAATGAATATTGTGGTGCTTATTTCCGGCAACGGAAGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGc > 4:480098/1‑149 (MQ=255) gAACAACGCGTGGTTATCGAATAATGAATATTGTGGTGCTTATTTCCGGCAACGGAAGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGc > 4:539810/1‑149 (MQ=255) aacaacGCGTGGTTATCGAATAATGAATATTGTGGTGCTTATTTCCGGCAACGGAAGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGcc < 2:898416/149‑1 (MQ=255) acaacGCGTGGTTATCGAATAATGAATATTGTGGTGCTTATTTCCGGCAACGGAAGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCGAACGCG‑TCGGCCt < 4:708934/149‑1 (MQ=255) acaacGCGTGGTTATCGAATAATGAATATTGTGGTGCTTATTTCCGGCAACGGAAGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCGAACGCG‑TCGGCCt < 1:481557/149‑1 (MQ=255) caacGCGTGGTTATCGAATAATGAATATTGTGGTGCTTATTTCCGGCAACGGAAGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCtt > 2:345002/1‑149 (MQ=255) acGCGTGGTTATCGAATAATGAATATTGTGGTGCTTATTTCCGGCAACGGAAGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGa < 3:496743/149‑1 (MQ=255) cgcgTGGTTATCGAATAATGAATATTGTGGTGCTTATTTCCGGCAACGGAAGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGaa < 3:373788/149‑1 (MQ=255) tGGTTATCGAATAATGAATATTGTGGTGCTTATTTCCGGCAACGGAAGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAAcgcg > 1:812063/1‑149 (MQ=255) cGAATAATGAATATTGTGGTGCTTATTTCCGGCAACGGAAGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCa < 1:883685/149‑1 (MQ=255) gAATAATGAATATTGTGGTGCTTATTTCCGGCAACGGAAGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAACTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAg > 3:350999/1‑149 (MQ=255) ataatGAATATTGTGGTGCTTATTTCCGGCAACGGAAGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGc > 3:377238/1‑149 (MQ=255) taatGAATATTGTGGTGCTTATTTCCGGCAACGGAAGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCg < 1:917565/149‑1 (MQ=255) tGAATATTGTGGTGCTTATTTCCGGCAACGGAAGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGt > 2:160570/1‑149 (MQ=255) aaTATTGTGGTGCTTATTTCCGGCAACGGAAGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTAt < 1:257596/149‑1 (MQ=255) atatTGTGGTGCTTATTTCCGGCAACGGAAGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTAtt < 2:473883/149‑1 (MQ=255) atatTGTGGTGCTTATTTCCGGCAACGGAAGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTAtt < 1:630692/149‑1 (MQ=255) ttGTGGTGCTTATTTCCGGCAACGGAAGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTa < 4:159537/144‑1 (MQ=255) ttGTGGTGCTTATTTCCGGCAACGGAAGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAACTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCa < 4:350999/149‑1 (MQ=255) ttGTGGTGCTTATTTCCGGCAACGGAAGGAATTTACAGGCAATTATTGACGCCTGTAAAACTAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCATTAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTa > 3:159537/1‑144 (MQ=255) gtggtgCTTATTTCCGGCAACGGAAGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAAc < 1:502863/149‑1 (MQ=255) tttCCGGCAACGGAAGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTc > 4:135851/1‑149 (MQ=255) ccGGCAACGGAAGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATc < 1:345002/149‑1 (MQ=255) cGGCAACGGAAGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCg > 4:766611/1‑149 (MQ=255) cAACGGAAGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCa < 2:812985/149‑1 (MQ=255) gAAGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTAtt > 1:492194/1‑122 (MQ=255) gAAGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTAtt < 2:492194/122‑1 (MQ=255) gAAGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGcgcg > 3:104054/1‑149 (MQ=255) aaGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGTGTATTGCAACGCATACGCTCATCGCCAGCGCGt < 3:480098/149‑1 (MQ=255) gTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGttt < 4:509362/149‑1 (MQ=255) tACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGt < 1:323973/149‑1 (MQ=255) gCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGa > 4:636414/1‑149 (MQ=255) gCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGa > 3:429948/1‑149 (MQ=255) gCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGa > 2:848051/1‑149 (MQ=255) attattGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGc < 3:496327/149‑1 (MQ=255) ttattGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGcc > 2:428995/1‑149 (MQ=255) attGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTa > 1:772191/1‑149 (MQ=255) ttGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTAt < 1:428995/149‑1 (MQ=255) ttGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTAt < 3:357482/149‑1 (MQ=255) aCGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGAc > 3:238502/1‑149 (MQ=255) gCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCg > 4:565320/1‑149 (MQ=255) gCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCg > 1:777823/1‑149 (MQ=255) gCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCg > 2:428740/1‑149 (MQ=255) ccTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCgg < 3:765895/149‑1 (MQ=255) ccTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCgg < 3:126365/149‑1 (MQ=255) tAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTTAACGCGCCCGCCAGGCGGTGATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGTAGt < 4:586218/149‑1 (MQ=255) aaaCCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCGAACGCG‑TCGGCCtta > 4:29517/1‑65 (MQ=37) aaaCCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCGAACGCG‑TCGGCCtta < 3:29517/66‑2 (MQ=37) aaaCCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTg > 1:929069/1‑149 (MQ=255) aCCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGAt < 3:241356/149‑1 (MQ=255) aCCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGAt < 1:446117/149‑1 (MQ=255) caacaaAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCgg < 3:126960/80‑1 (MQ=255) caacaaAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCgg > 4:126960/1‑80 (MQ=255) caacaaAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTc > 3:906469/1‑149 (MQ=255) aacaaAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGcc > 1:403759/1‑129 (MQ=255) aacaaAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGcc < 2:403759/129‑1 (MQ=255) aacaaAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCa > 3:870984/1‑149 (MQ=255) aacaaAATTAAAGGCACCGTACGGGCAGTTGTCAGAAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCGCGCCATGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGAGAAGCCTATGACCGGGAGTTGATTCa > 4:774716/1‑149 (MQ=255) aaaaTTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCATGa > 4:378415/1‑149 (MQ=255) aaTTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTc > 1:781048/1‑143 (MQ=255) aaTTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTc < 2:781048/143‑1 (MQ=255) aTTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCATGAAAt > 1:104319/1‑149 (MQ=255) aTTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCATGAAAt > 2:287184/1‑149 (MQ=255) ttAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCATGAAATc > 3:648731/1‑149 (MQ=255) ttAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCATGAAATc < 1:478433/149‑1 (MQ=255) ttAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCATGAAATc > 3:688170/1‑149 (MQ=255) aaGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCATGAAATCGAc < 4:429948/149‑1 (MQ=255) gggCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCATGAAATCGACATGTACGCAccc > 1:857123/1‑149 (MQ=255) ggCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGtt > 2:631951/1‑120 (MQ=255) ggCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGtt < 1:631951/120‑1 (MQ=255) ggCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCATGAAATCGACATGTACGCACCCg > 1:422657/1‑149 (MQ=255) cAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCATGAAATCGACATGTACGCACCCGAt < 3:636414/149‑1 (MQ=255) gTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCATGAAATCGACATGTACGCACCCGAtgt < 3:822118/149‑1 (MQ=255) gTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCATGAAATCGACATGTACGCACCCGAtgt < 4:360345/149‑1 (MQ=255) ttttCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCATGAAATCGACATGTACGCACCCGAtgtg > 2:536105/1‑149 (MQ=255) tttCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCATGAAATCGACATGTACGCACCCGATGTgg < 4:648731/149‑1 (MQ=255) ttCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCATGAAATCGACATGTACGCACCCGATGTGGt > 4:446303/1‑149 (MQ=255) aGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCATGAAATCGACATGTACGCACCCGATGTGGTCGt > 3:684155/1‑149 (MQ=255) aGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCATGAAATCGACATGTACGCACCCGATGTGGTCGt > 2:317933/1‑149 (MQ=255) cAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCATGAAATCGACATGTACGCACCCGATGTGGTCGTgc < 2:857123/149‑1 (MQ=255) aaTAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCATGAAATCGACATGTACGCACCCGATGTGGTCGTgct > 2:2926/1‑149 (MQ=255) aaGGCCGAACGCG‑TCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCATGAAATCGACATGTACGCACCCGATGTGGTCGTgctggc > 4:350171/1‑149 (MQ=255) aGGCCGAACGCG‑TCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCATGAAATCGACATGTACGCACCCGATGTGGTCGTgctggct > 2:227854/1‑149 (MQ=255) aGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCATGAAATCGACATGTACGCACCCGATGTGGTCGTgctggct > 4:676359/1‑149 (MQ=255) gCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCATGAAATCGACATGTACGCACCCGATGTGGTCGTGctggctgg < 4:238502/149‑1 (MQ=255) g‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACa > 3:381892/1‑73 (MQ=255) g‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACa < 4:381892/73‑1 (MQ=255) g‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCATGAAATCGACATGTACGCACCCGATGTGGTCGTGCTGGCTGGttt < 4:469026/149‑1 (MQ=255) | TCTGATTCCGAACAACGCGTGGTTATCGAATAATGAATATTGTGGTGCTTATTTCCGGCAACGGAAGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCATGAAATCGACATGTACGCACCCGATGTGGTCGTGCTGGCTGGTTT > NC_000913/2622202‑2622496 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |