Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 3,787,624:1 | +A | 5.2% | coding (787/1260 nt) | envC → | activator of AmiB,C murein hydrolases, septal ring factor |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 3,787,624 | 1 | . | A | 5.2% | 412.5 / 16.9 | 97 | coding (787/1260 nt) | envC | activator of AmiB,C murein hydrolases, septal ring factor |
Reads supporting (aligned to +/- strand): ref base . (44/48); new base A (2/3); total (46/51) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.42e-01 |
Only 100 of 103 total aligned reads displayed.
AGGGCTGGAGTCTTCCATCCAGCAAGGTCAGCAACAGTTGAGCGAGCTGCGCGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCG‑ACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGTACCGGTGGTCTGGGCGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGC > NC_000913/3787476‑3787770 | aGGGCTGGAGTCTTCCATCCAGCAAGGTCAGCAACAGTTGAGCGAGCTGCGCGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTcgcg > 4:152006/1‑149 (MQ=255) aGGGCTGGAGTCTTCCATCCAGCAAGGTCAGCAACAGTTGAGCGAGCTGCGCGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTcgcg > 4:469063/1‑149 (MQ=255) aGGGCTGGAGTCTTCCATCCAGCAAGGTCAGCAACAGTTGAGCGAGCTGCGCGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTcgcg > 1:356183/1‑149 (MQ=255) gggCTGGAGTCTTCCATCCAGCAAGGTCAGCAACAGTTGAGCGAGCTGCGCGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCG‑a < 1:397674/149‑1 (MQ=255) gAGTCTTCCATCCAGCAAGGTCAGCAACAGTTGAGCGAGCTGCGCGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCG‑ACCGCCa > 1:528443/1‑149 (MQ=255) gAGTCTTCCATCCAGCAAGGTCAGCAACAGTTGAGCGAGCTGCGCGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGG‑TCGCGAACCGcct < 4:188721/149‑2 (MQ=255) cTTCCATCCAGCAAGGTCAGCAACAGTTGAGCGAGCTGCGCGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCG‑ACCGCCagaaa < 4:543963/149‑1 (MQ=255) ttCCATCCAGCAAGGTCAGCAACAGTTGAGCGAGCTGCGCGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCG‑ACCGCCagaaag > 2:520604/1‑149 (MQ=255) ttCCATCCAGCAAGGTCAGCAACAGTTGAGCGAGCTGCGCGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCG‑ACCGCCagaaag > 4:231035/1‑149 (MQ=255) ttCCATCCAGCAAGGTCAGCAACAGTTGAGCGAGCTGCGCGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGG‑TCGCGAACCGcct > 4:86563/1‑143 (MQ=255) ttCCATCCAGCAAGGTCAGCAACAGTTGAGCGAGCTGCGCGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGG‑TCGCGAACCGcct < 3:86563/144‑2 (MQ=255) tccatccaGCAAGGTCAGCAACAGTTGAGCGAGCTGCGCGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCG‑ACCGCCagaaaga < 1:636664/149‑1 (MQ=255) ccatccaGCAAGGTCAGCAACAGTTGAGCGAGCTGCGCGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCG‑ACCGCCagaacgaa < 2:592543/149‑1 (MQ=255) ccatccaGCAAGGTCAGCAACAGTTGAGCGAGCTGCGCGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCG‑ACCGCCagaaagaa > 1:376963/1‑149 (MQ=255) catccaGCAAGGTCAGCAACAGTTGAGCGAGCTGCGCGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCG‑ACCGCCAGAaagaag < 1:185590/149‑1 (MQ=255) atccaGCAAGGTCAGCAACAGTTGAGCGAGCTGCGCGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCG‑ACCGCCAGAAAGAAGc > 1:478497/1‑149 (MQ=255) atccaGCAAGGTCAGCAACAGTTGAGCGAGCTGCGCGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCG‑ACCGCCAGAAAGAAGc > 3:877412/1‑149 (MQ=255) atccaGCAAGGTCAGCAACAGTTGAGCGAGCTGCGCGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCG‑ACCGCCAGAAAGAAGc > 1:646528/1‑149 (MQ=255) ccaGCAAGGTCAGCAACAGTTGAGCGAGCTGCGCGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCG‑ACCGCCAGAAAGAAGCGa < 2:528443/149‑1 (MQ=255) aGCAAGGTCAGCAACAGTTGAGCGAGCTGCGCGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCG‑ACCGCCAGAAAGAAGCGAcg < 3:152006/149‑1 (MQ=255) ggTCAGCAACAGTTGAGCGAGCTGCGCGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCG‑ACCGCCAGAAAGAAGCGACGCGCaa > 1:147992/1‑149 (MQ=255) ggTCAGCAACAGTTGAGCGAGCTGCGCGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCG‑ACCGCCAGAAAGAAGCGACGCGCaa > 2:850290/1‑149 (MQ=255) gTCAGCAACAGTTGAGCGAGCTGCGCGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCG‑ACCGCCAGAAAGAAGCGACGCGCaaa < 2:491933/149‑1 (MQ=255) aGCAACAGTTGAGCGAGCTGCGCGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCTAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCG‑ACCGCCAGAAAGAAGCGACGCGCAAAGGc > 3:288230/1‑149 (MQ=255) aGCAACAGTTGAGCGAGCTGCGCGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCG‑ACCGCCAGAAAGAAGCGACGCGCAAAGGc < 4:877412/149‑1 (MQ=255) aGCAACAGTTGAGCGAGCTGCGCGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCG‑ACCGCCAGAAAGAAGCGACGCGCAAAGGc < 2:478497/149‑1 (MQ=255) gCAACAGTTGAGCGAGCTGCGCGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCG‑ACCGCCAGAAAGAAGCGACGCGCAAAGGca > 4:114693/1‑149 (MQ=255) aaCAGTTGAGCGAGCTGCGCGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCG‑ACCGCCAGAAAGAAGCGACGCGCAAAGGcacc > 4:772532/1‑149 (MQ=255) aCAGTTGAGCGAGCTGCGCGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCG‑ACCGCCAGAAAGAAGCGACGCGCAAAGGcacca > 3:144814/1‑149 (MQ=255) aCAGTTGAGCGAGCTGCGCGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGGTCGCG‑ACCGCCAGAAAGAAGCGACGCGCAAAGGcacca > 2:217244/1‑149 (MQ=255) cAGTTGAGCGAGCTGCGCGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCG‑ACCGCCAGAAAGAAGCGACGCGCAAAGGcaccac < 3:445343/149‑1 (MQ=255) gTTGAGCGAGCTGCGCGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCG‑ACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCt > 2:779618/1‑149 (MQ=255) ttGAGCGAGCTGCGCGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCG‑ACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTa < 2:697444/149‑1 (MQ=255) gcTGCGCGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGG‑TCGCGAACCGcctgg > 1:630906/1‑110 (MQ=255) gcTGCGCGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGG‑TCGCGAACCGcctgg < 2:630906/113‑4 (MQ=255) cgcCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCG‑ACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGaaa < 1:509117/149‑1 (MQ=255) cgcCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCG‑ACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGaaa < 2:356183/149‑1 (MQ=255) gcCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCG‑ACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAg > 4:558265/1‑149 (MQ=255) gcCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCG‑ACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAg < 3:165996/149‑1 (MQ=255) cAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCG‑ACCGCCAGAaagaag > 3:21417/1‑110 (MQ=255) cAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCG‑ACCGCCAGAaagaag < 4:21417/110‑1 (MQ=255) aaCGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCG‑ACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAg < 4:500484/146‑1 (MQ=255) aaCGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCG‑ACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAg > 3:500484/1‑146 (MQ=255) aaCGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCG‑ACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGa > 2:179054/1‑149 (MQ=255) aCGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCG‑ACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGaa < 1:779618/149‑1 (MQ=255) cGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCG‑ACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGaaa > 3:850989/1‑149 (MQ=255) aaTCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCG‑ACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGaaaaa > 2:745856/1‑149 (MQ=255) tCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCG‑ACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAacc < 2:288202/131‑1 (MQ=255) tCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCG‑ACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAacc > 1:288202/1‑131 (MQ=255) ccGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCG‑ACCGCCAGAAAGAAGCGAc > 1:708630/1‑105 (MQ=255) ccGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCG‑ACCGCCAGAAAGAAGCGAc < 2:708630/105‑1 (MQ=255) ccGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCG‑ACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGc < 3:740789/149‑1 (MQ=255) ccGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCG‑ACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGc < 3:61927/149‑1 (MQ=255) ccGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCG‑ACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGc < 2:721457/149‑1 (MQ=255) cGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCGCAGGCGGTTCGCG‑ACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCt < 3:629169/149‑1 (MQ=255) cGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCG‑ACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCt < 1:179054/149‑1 (MQ=255) tCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCG‑ACCGCCAGAAAGAAGCGACGCGCAAAGGc < 4:497545/112‑1 (MQ=255) tCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCG‑ACCGCCAGAAAGAAGCGACGCGCAAAGGc > 3:497545/1‑112 (MQ=255) tCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCG‑ACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGa < 3:558265/149‑1 (MQ=255) tGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCG‑ACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGaaa < 3:595314/132‑1 (MQ=255) tGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCG‑ACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGaaa > 4:595314/1‑132 (MQ=255) cGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCG‑ACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGc < 4:318362/142‑1 (MQ=255) cGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCG‑ACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGc > 3:318362/1‑142 (MQ=255) tAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCG‑ACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTccc < 2:734786/149‑1 (MQ=255) aCAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCG‑ACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTAc < 1:454473/113‑1 (MQ=255) aCAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCG‑ACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTAc > 2:454473/1‑113 (MQ=255) cAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCG‑ACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGTa > 1:526688/1‑149 (MQ=255) aGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCG‑ACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGTAc > 1:140695/1‑149 (MQ=255) aGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCG‑ACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGTAc > 3:770780/1‑149 (MQ=255) aGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCG‑ACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGTAc > 3:275262/1‑149 (MQ=255) aTTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCG‑ACCGCCAGAAAGAAGCGACGCGCAAAgg < 3:267808/98‑1 (MQ=255) aTTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCG‑ACCGCCAGAAAGAAGCGACGCGCAAAgg > 4:267808/1‑98 (MQ=255) aTTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCG‑ACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGTACCgg > 1:678974/1‑149 (MQ=255) cccGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCG‑ACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGTACCggtggt > 1:907378/1‑149 (MQ=255) cGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCG‑ACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGTACCGGTGGTCt < 1:850290/149‑1 (MQ=255) gTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCG‑ACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGTACCGGTGGTCTg > 3:864368/1‑149 (MQ=255) tGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCG‑ACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGTACCGGTGGTCTgg < 2:140695/149‑1 (MQ=255) tGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCG‑ACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGTACCGGTGGTCTgg > 2:79545/1‑149 (MQ=255) ggcgAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCG‑ACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGTACCGGTGGTCTGGGCGCGCCGCGTg < 1:198393/149‑1 (MQ=255) cgAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCG‑ACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGTACCGGTGGTCTGGGCGCGCCGCGTGGt < 2:92229/149‑1 (MQ=255) aaGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCG‑ACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGTACCGGTGGTCTGGGCGCGCCGCGTGGTCAg < 1:520604/149‑1 (MQ=255) gCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCG‑ACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGTACCGGTGGTCTGGGCGCGCCGCGTGGTCAGGCATTCTg < 3:406734/149‑1 (MQ=255) aGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCG‑ACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGTACCGGTGGTCTGGGCGCGCCGCGTGGTCAGGCATTCTGGc < 3:772532/149‑1 (MQ=255) gAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCG‑ACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAaccga > 1:923455/1‑79 (MQ=255) gAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCG‑ACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAaccga < 2:923455/79‑1 (MQ=255) gAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCG‑ACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGTACCGGTGGTCTGGGCGCGCCGCGTGGTCAGGCATTCTGGcc > 3:188649/1‑149 (MQ=255) aaCGAGAAGCACGTGAGGCCCAGGCGGTTCGCG‑ACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGTACCGGTGGTCTGGGCGCGCCGCGTGGTCAGGCATTCTGGCCg > 1:516864/1‑149 (MQ=255) aCGAGAAGCACGTGAGGCCCAGGCGGTTCGCG‑ACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGTACCGGTGGTCTGGGCGCGCCGCGTGGTCAGGCATTCTGGCCgg < 1:79545/149‑1 (MQ=255) gagaAGCACGTGAGGCCCAGGCGGTTCGCG‑ACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGTACCGGTGGTCTGGGCGCGCCGCGTGGTCAGGCATTCTGGCCGGtt > 3:419005/1‑149 (MQ=255) gaAGCACGTGAGGCCCAGGCGGTTCGCG‑ACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGTACCGGTGGTCTGGGCGCGCCGCGTGGTCAGGCATTCTGGCCGGTTcg > 3:207595/1‑149 (MQ=255) aaGCACGTGAGGCCCAGGCGGTTCGCG‑ACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGTACCGGTGGTCTGGGCGCGCCGCGTGGTCAGGCATTCTGGCCGGTTcgc > 1:260059/1‑149 (MQ=255) aaGCACGTGAGGCCCAGGCGGTTCGCG‑ACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGTACCGGTGGTCTGGGCGCGCCGCGTGGTCAGGCATTCTGGCCGGTTcgc < 3:863462/149‑1 (MQ=255) gCACGTGAGGCCCAGGCGGTTCGCG‑ACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGTACCGGTGGTCTGGGCGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCgg < 3:231035/149‑1 (MQ=255) aCGTGAGGCCCAGGCGGTTCGCG‑ACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGTACCGGTGGTCTGGGCGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGc < 4:334607/149‑1 (MQ=255) cccAGGCGGTTCGCG‑ACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGTACCGGTGGTCTGGGCGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTg > 1:73090/1‑149 (MQ=255) ccAGGCGGTTCGCG‑ACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGTACCGGTGGTCTGGGCGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGc > 4:108518/1‑140 (MQ=255) ccAGGCGGTTCGCG‑ACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGTACCGGTGGTCTGGGCGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGc < 3:108518/140‑1 (MQ=255) ttCGCG‑ACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGTACCGGTGGTCTGGGCGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTAt < 3:384899/149‑1 (MQ=255) cgcg‑ACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGTACCGGTGGTCTGGGCGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATgg < 4:127732/149‑1 (MQ=255) gcg‑ACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCAAAAAATCGCTGATGTCCCGTACCGGTGGTCTGGGCGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGc < 1:654624/149‑1 (MQ=255) | AGGGCTGGAGTCTTCCATCCAGCAAGGTCAGCAACAGTTGAGCGAGCTGCGCGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCG‑ACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGTACCGGTGGTCTGGGCGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGC > NC_000913/3787476‑3787770 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |