Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 95,071 T→C 5.5% V141A (GTA→GCA) ‡ murF → UDP‑N‑acetylmuramoyl‑tripeptide:D‑alanyl‑D‑ alanine ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091395,0710TC 5.5% 361.1 / 7.8 109V141A (GTA→GCA) ‡murFUDP‑N‑acetylmuramoyl‑tripeptide:D‑alanyl‑D‑ alanine ligase
Reads supporting (aligned to +/- strand):  ref base T (53/50);  new base C (3/3);  total (56/53)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.96e-01

Only 100 of 111 total aligned reads displayed.

GTTCGCCAGCAAGTTCCGGCGCGCGTGGTTGCTCTGACGGGGTCCTCCGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAGGGCGAAATAGCCTGGACTGTGAGTCTGACTCGCCCGGAAGCTGCGCTGGTCAACAACCTGGCAGCGGCGCATCTGGAA  >  NC_000913/94929‑95219
                                                                                                                                              |                                                                                                                                                    
gTTCGCCAGCAAGTTCCGGCGCGCGTGGTTGCTCTGACGGGGTCCTCCGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGAt                                                                                                                                                <  4:816175/149‑1 (MQ=255)
gTTCGCCAGCAAGTTCCGGCGCGCGTGGTTGCTCTGACGGGGTCCTCCGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGAt                                                                                                                                                <  4:223915/149‑1 (MQ=255)
    gCCAGCAAGTTCCGGCGCGCGTGGTTGCTCTGACGGGGTCCTCCGGAAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACg                                                                                                                                            >  1:216874/1‑149 (MQ=255)
      cAGCAAGTTCCGGCGCGCGTGGTTGCTCTGACGGGGTCCTCCGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCt                                                                                                                                          >  2:307935/1‑149 (MQ=255)
       aGCAAGTTCCGGCGCGCGTGGTTGCTCTGACGGGGTCCTCCGGCAAAACCTCCGTTAAAGAGATGCCGCCGGCGATTTTAAGCCGGTGCGGCAACAGGCTTATTACGGCGGCCAACCTAAACAACGACATGGGTGTACGGATGACGcgg                                                                                                                                         >  2:745535/1‑147 (MQ=255)
         cAAGTTCCGGCGCGCGTGGTTGCTCTGACGGGGTCCTCCGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAGCGTCATCGGTACACCGATGTCgttgtt                                                                                                                                       <  4:163244/149‑1 (MQ=255)
         cAAGTTCCGGCGCGCGTGGTTGCTCTGACGGGGTCCTCCGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGtt                                                                                                                                       <  2:381266/149‑1 (MQ=255)
            gTTCCGGCGCGCGTGGTTGCTCTGACGGGGTCCTCCGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTgcg                                                                                                                                    >  2:163773/1‑149 (MQ=255)
             ttCCGGCGCGCGTGGTTGCTCTGACGGGGTCCTCCGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTgcgc                                                                                                                                   >  2:664838/1‑149 (MQ=255)
                      gcgTGGTTGCTCTGACGGGGTCCTCCGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGACTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCg                                                                                                                          >  1:360254/1‑149 (MQ=255)
                           gTTGCTCTGACGGGGTCCTCCGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCgg                                                                                                                         <  3:400471/145‑1 (MQ=255)
                           gTTGCTCTGACGGGGTCCTCCGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATa                                                                                                                     >  1:778541/1‑149 (MQ=255)
                             tGCTCTGACGGGGTCCTCCGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTAc                                                                                                                                                    <  3:411504/116‑1 (MQ=255)
                             tGCTCTGACGGGGTCCTCCGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTAc                                                                                                                                                    >  4:411504/1‑116 (MQ=255)
                               ctctGACGGGGTCCTCCGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGAt                                                                                                                 <  4:902155/149‑1 (MQ=255)
                                tctGACGGGGTCCTCCGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGAtt                                                                                                                >  2:836016/1‑149 (MQ=255)
                                tctGACGGGGTCCTCCGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCGGTTGCGCTTAACGCCGGAATACGAtt                                                                                                                >  1:373641/1‑149 (MQ=255)
                                    aCGGGGTCCTCCGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGc                                                                                                            >  1:251503/1‑149 (MQ=255)
                                     cGGGGTCCTCCGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGGTGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCa                                                                                                           <  4:763355/149‑1 (MQ=255)
                                      ggggTCCTCCGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAg                                                                                                          <  1:84147/149‑1 (MQ=255)
                                        ggTCCTCCGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGtt                                                                                                        >  4:82869/1‑149 (MQ=255)
                                        ggTCCTCCGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGtt                                                                                                        >  1:638308/1‑149 (MQ=255)
                                        ggTCCTCCGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACCCTGTTGCGCTTAACGCCGGAATACGATTACGCAGtt                                                                                                        <  4:876003/149‑1 (MQ=255)
                                        ggTCATCCGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGtt                                                                                                        <  4:27850/149‑1 (MQ=255)
                                          tcctccGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTAt                                                                                                      >  2:843449/1‑149 (MQ=255)
                                          tcctccGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTAt                                                                                                      >  3:605769/1‑149 (MQ=255)
                                           cctccGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTAtt                                                                                                     <  1:478798/149‑1 (MQ=255)
                                            ctccGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTg                                                                                                    >  1:334218/1‑149 (MQ=255)
                                            ctccGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTg                                                                                                    <  2:251503/149‑1 (MQ=255)
                                             tccGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCgg                                                                                                                         >  3:626127/1‑127 (MQ=255)
                                             tccGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTAtt                                                                                                     <  1:822406/147‑1 (MQ=255)
                                             tccGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTAtt                                                                                                     >  2:822406/1‑147 (MQ=255)
                                             tccGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGa                                                                                                   >  2:144442/1‑149 (MQ=255)
                                             tccGGCAAAACCTCAGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCgg                                                                                                                         <  4:626127/127‑1 (MQ=255)
                                                 gCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGGCAGTGCGGCAACACGCTTTATACGGCAGGCAATCACAACAACGACAACGGTGTACCGATGACTCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTCATTGACCtt                                                                                               <  4:585770/149‑1 (MQ=255)
                                                 gCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACtt                                                                                               >  3:582862/1‑149 (MQ=255)
                                                  cAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTg                                                                                              >  2:513680/1‑149 (MQ=255)
                                                   aaaaCCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAGCGTCATCGGTACACCGATGTCGttgttga                                                                                                                                     >  1:662794/1‑108 (MQ=37)
                                                    aaaCCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGaa                                                                                                  <  2:388246/143‑1 (MQ=255)
                                                    aaaCCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGaa                                                                                                  >  1:388246/1‑143 (MQ=255)
                                                        cTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGa                                                                                        >  1:545369/1‑149 (MQ=255)
                                                        cTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGa                                                                                        >  1:286906/1‑149 (MQ=255)
                                                        cTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGa                                                                                        >  1:822864/1‑149 (MQ=255)
                                                         tCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGaa                                                                                       >  3:64355/1‑149 (MQ=255)
                                                          ccGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAAc                                                                                      <  1:843449/149‑1 (MQ=255)
                                                            gTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATa                                                                                                                     >  4:776831/1‑116 (MQ=255)
                                                             ttAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCAt                                                                                   >  2:687742/1‑149 (MQ=255)
                                                                aaGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAg                                                                                <  2:80386/149‑1 (MQ=255)
                                                                 agagATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAgg                                                                               <  1:745535/149‑1 (MQ=255)
                                                                 agagATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAgg                                                                               <  1:163773/149‑1 (MQ=255)
                                                                 agagATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAgg                                                                               <  1:604143/149‑1 (MQ=255)
                                                                 agagATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAgg                                                                               <  2:778541/149‑1 (MQ=255)
                                                                      tGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAGGGCGaa                                                                          >  2:831216/1‑149 (MQ=255)
                                                                           gcggcgATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAGGGCGAAATAGc                                                                     <  1:852238/149‑1 (MQ=255)
                                                                             ggcgATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGATTACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAGGGCGAAATAGCCt                                                                   <  4:582862/149‑1 (MQ=255)
                                                                                      aaGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAGGGCGAAATAGCCTGGACTGTGa                                                          <  3:487811/149‑1 (MQ=255)
                                                                                        gCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAGGGCGAAATAGCCTGGACTGTGAGt                                                        >  3:594635/1‑149 (MQ=255)
                                                                                        gCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAGGGCGAAATAGCCTGGACTGTGAGt                                                        <  1:664838/149‑1 (MQ=255)
                                                                                        gCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAGGGCGAAATAGCCTGGACTGTGAGt                                                        >  3:305475/1‑149 (MQ=255)
                                                                                           aGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCgg                                                                                                                         <  4:58663/81‑1 (MQ=255)
                                                                                           aGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCgg                                                                                                                         >  3:58663/1‑81 (MQ=255)
                                                                                            gTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAGGGCGAAATAGCCTGGACTGTGAGTCTGa                                                    <  1:825861/149‑1 (MQ=255)
                                                                                            gTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAGGGCGAAATAGCCTGGACTGTGAGTCTGa                                                    >  1:707390/1‑149 (MQ=255)
                                                                                             tGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACCACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCAGCAGGGCGAAATAGCCAGGACTGTGAGTCTGAc                                                   <  2:180572/149‑1 (MQ=255)
                                                                                                ggCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAGGGCGAAATAGCCTGGACTGTGAGTCTGACTCg                                                >  3:154089/1‑149 (MQ=255)
                                                                                                ggCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAGGGCGAAATAGCCTGGACTGTGAGTCTGACTCg                                                >  4:555152/1‑149 (MQ=255)
                                                                                                  cAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAGGGCGAAATAGCCTGGACTGTGAGTCTGACTCGcc                                              >  1:760034/1‑149 (MQ=255)
                                                                                                   aaCACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAGCTTGGCGCGAACCATCAGGGCGAAATAGCCTGGAATATGAGTCTGACTCGccc                                             >  1:722436/1‑149 (MQ=255)
                                                                                                   aaCACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAGGGCGAAATAGCCTGGACTGTGAGTCTGACTCGccc                                             <  3:818517/149‑1 (MQ=255)
                                                                                                   aaCACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAGGGCGAAATAGCCTGGACTGTGAGTCTGACTCGccc                                             <  1:170064/149‑1 (MQ=255)
                                                                                                    acacGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAGGGc                                                                             >  3:462045/1‑116 (MQ=255)
                                                                                                    acacGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAGGGc                                                                             <  4:462045/116‑1 (MQ=255)
                                                                                                    acacGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAGGGCGAAATAGCCTGGACTGTGAGTCTGACTCGCCCg                                            >  2:26549/1‑149 (MQ=255)
                                                                                                     cacGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAGGGCGAAATAGCCTGGACTGTGAGTCTGACTCGCCCgg                                           >  1:740475/1‑149 (MQ=255)
                                                                                                      acGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAGGGCGAAATAGc                                                                     <  3:847443/122‑1 (MQ=255)
                                                                                                      acGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAGGGCGAAATAGc                                                                     >  4:847443/1‑122 (MQ=255)
                                                                                                      acGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAGGGCGAAATAGCCTGGACTGTGAGTCTGACTCGCCCGGa                                          <  2:760034/149‑1 (MQ=255)
                                                                                                       cGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGGCGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAGGGCTAAATAGCCTGGACtgt                                                            >  3:631872/1‑130 (MQ=255)
                                                                                                       cGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCAGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAGGGCGAAATAGCCTGGACtgt                                                            <  4:631872/130‑1 (MQ=255)
                                                                                                           ttATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAGGGCGAAATAGCCTGGACTGTGAGTCTGACTCGCCCGGAAGCTg                                     <  4:154089/149‑1 (MQ=255)
                                                                                                                 ggcaggcaATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAGGGCGAAATAGCCTGGACTGTGAGTCTGACTCGCCCGGAAGCTGCGCTgg                               >  4:690417/1‑149 (MQ=255)
                                                                                                                      gcaATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAGGGCGAAATAGCCTGGACTGTGAGTCTGACTCGCCCGGAAGCTGCGCTGGTcaac                          <  4:461139/149‑1 (MQ=255)
                                                                                                                       caATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAGGGCGAAATAGCCTGGACt                                                              <  3:395709/112‑1 (MQ=255)
                                                                                                                       caATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAGGGCGAAATAGCCTGGACt                                                              >  4:395709/1‑112 (MQ=255)
                                                                                                                        aaTCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGaa                                                                                                                       <  2:420334/54‑1 (MQ=255)
                                                                                                                           ctcAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAGGGCGAAATAGCCTGGACTGTGAGTCTGACTCGCCCGGAAGCTGCGCTGGTCAACAACCt                     <  1:26549/149‑1 (MQ=255)
                                                                                                                           ctcAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAGGGCGAAATAGCCTGGACTGTGAGTCTGACTCGCCCGGAAGCTGCGCTGGTCAACAACCt                     <  2:334218/149‑1 (MQ=255)
                                                                                                                            tcAACAACGACCTCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAGGGCGAAATAGCCTGGACTGTGAGTCTGACTCGCCCGGAAGCTGCGCTGGTCAACAACCTg                    >  1:693751/1‑149 (MQ=255)
                                                                                                                            tcAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAGGGCGAAATAGCCTGGACTGTGAGTCTGACTCGCCCGGAAGCTGCGCTGGTCAACAACCTg                    >  1:669709/1‑149 (MQ=255)
                                                                                                                            gcaacagCGTCATCGGTACACCGATGTCGTTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAGGGCGAAATAGCCTGGACTGTGAGTCTGAc                                                   <  1:794172/117‑1 (MQ=37)
                                                                                                                            acaacagCGTCATCGGTACACCGATGTCGTTGTTGCGCTTAACGCCAGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAGGGCGAAATAGCCTGGACTGTGAGTCTGAc                                                   >  2:794172/2‑118 (MQ=37)
                                                                                                                             caacaaCGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAGGGCGAAATAGCCTGGACTGTGAGTCTGACTCGCCCGGAAGCTGCGCTGGTCAACAACCTgg                   >  2:159598/1‑149 (MQ=255)
                                                                                                                              aacaacGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAGGGCGAAATAGCCTGGACTGTGAGTCTGACTCGCCCGGAAGCTGCGCTGGTCAACAACCTg                    >  1:894028/1‑147 (MQ=255)
                                                                                                                              aacaacGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAGGGCGAAATAGCCTGGACTGTGAGTCTGACTCGCCCGGAAGCTGCGCTGGTCAACAACCTg                    <  2:894028/147‑1 (MQ=255)
                                                                                                                              aacaacGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAGGGCGAAATAGCCTGGACTGTGAGTCTGACTCGCCCGGAAGCTGCGCTGGTCAACAACCTGGc                  <  2:360254/149‑1 (MQ=255)
                                                                                                                                  acGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAGGGCGAAATAGCCTGGACTGTGAGTCTGACTCGCCCGGAAGCTGCGCTGGTCAACAACCTGGCAgcg              <  2:740475/149‑1 (MQ=255)
                                                                                                                                     tcATCGGTACACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAGGGCGAAATAGCCTGGACTGTGAGTCTGACTCGCCCGGAAGCTGCGCTGGTCAACAACCTGGCAgcggcg           >  4:39861/2‑149 (MQ=255)
                                                                                                                                       aTCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAGGGCGAAATAGCCTGGa                                                                <  3:867714/94‑1 (MQ=255)
                                                                                                                                       aTCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAGGGCGAAATAGCCTGGa                                                                >  4:867714/1‑94 (MQ=255)
                                                                                                                                       aTCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAGGGCGAAATAGCCTGGACTGTGAGTCTGACTCGCCCGGAAGCTGCGCTGGTCATCAACCTGGCAGCGGCGCa         >  1:915584/1‑149 (MQ=255)
                                                                                                                                              |                                                                                                                                                    
GTTCGCCAGCAAGTTCCGGCGCGCGTGGTTGCTCTGACGGGGTCCTCCGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAGGGCGAAATAGCCTGGACTGTGAGTCTGACTCGCCCGGAAGCTGCGCTGGTCAACAACCTGGCAGCGGCGCATCTGGAA  >  NC_000913/94929‑95219

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: