Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 799,679 G→T 100% D299E (GAC→GAA ybhD ← putative DNA‑binding transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913799,6790GT100.0% 427.8 / NA 114D299E (GAC→GAAybhDputative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (59/55);  total (59/55)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 114 total aligned reads displayed.

CTATGCGGTCGGGCAGGGACCAATGTGGGTGGTGGTTAACGCACACTAACCGCTGATTTACCCGGCGCAGTCTCTCCTGCGCCGGTGTATTAACCTATCTCCTGTAACGCGTGTCTCTGGCGTTCGACGATATTGGTCCACAAATTGTCTTTATCGTCAGTCCATAAATTAATCAGCAAGGCAAAAAAGCGTTCTGCTGCTGGAGAAAGTACGGCATCTTTACGGCGAATTATTCCCAATGTTCGACGTATAACGGGTTCCACCAATGGGATACCAATAATCGAAGAATAGG  >  NC_000913/799533‑799824
                                                                                                                                                  |                                                                                                                                                 
cTATGCGGTCGGGCAGGGACCAATGTGGGTGGTGGTTAACGCACACTAACCGCTGATTTACCCGGCGCAGTCTCTCCTGCGCCGGTGTATTAACCTATCTCCTGTAACGCGTGTCTCTGGCGTTCGACGATATTGGTCCACAAATTTTc                                                                                                                                                 <  1:404540/149‑1 (MQ=255)
 tATGCGGTCGGGCAGGGACCAATGTGGGTGGTGGTTAACGCACACTAACCGCTGATTTACCCGGCGCAGTCTCTCCTGCGCCGGTGTATTAACCTATCTCCTGTAACGCGTGTCTCTGGCGTTCGACGATATTGGTCCACAAATTTTCt                                                                                                                                                <  4:304518/149‑1 (MQ=255)
 tATGCGGTCGGGCAGGGACCAATGTGGGTGGTGGTTAACGCACACTAACCGCTGATTTACCCGGCGCAGTCTCTCCTGCGCCGGTGTATTAACCTATCTCCTGTAACGCGTGTCTCTGGCGTTCGACGATATTGGTCCACAAATTTTCt                                                                                                                                                <  3:347973/149‑1 (MQ=255)
 tATGCGGTCGGGCAGGGACCAATGTGGGTGGTGGTTAACGCACACTAACCGCTGATTTACCCGGCGCAGTCTCTCCTGCGCCGGTGTATTAACCTATCTCCTGTAACGCGTGTCTCTGGCGTTCGACGATATTGGTCCACAAATTTTCt                                                                                                                                                >  1:883142/1‑149 (MQ=255)
 tATGCGGTCGGGCAGGGACCAATGTGGGTGGTGGTTAACGCACACTAACCGCTGATTTACCCGGCGCAGTCTCTCCTGCGCCGGTGTATTAACCTATCTCCTGTAACGCGTGTCTCTGGCGTTCGACGATATTGGTCCACAAATTTTCt                                                                                                                                                >  1:398153/1‑149 (MQ=255)
       gTCGGGCAGGGACCAATGTGGGTGGTGGTTAACGCACACTAACCGCTGATTTACCCGGCGCAGTCTCTCCTGCGCCGGTGTATTAACCTATCTCCTGTAACGCGTGTCTCTGGCGTTCGACGATATTGGTCCACAAATTTTCTTTATCg                                                                                                                                          >  4:691785/1‑149 (MQ=255)
       gTCGGGCAGGGACCAATGTGGGTGGTGGTTAACGCACACTAACCGCTGATTTACCCGGCGCAGTCTCTCCTGCGCCGGTGTATTAACCTATCTCCTGTAACGCGTGTCTCTGGCGTTCGACGATATTGGTCCACAAATTTTCTTTATCg                                                                                                                                          >  4:210744/1‑149 (MQ=255)
          gggCAGGGACCAATGTGGGTGGTGGTTAACGCACACTAACCGCTGATTTACCCGGCGCAGTCTCTCCTGCGCCGGTGTATTAACCTATCTCCTGTAACGCGTGTCTCTGGCGTTCGACGATATTGGTCCACAAATTTTCTTTATCGTCa                                                                                                                                       >  4:533491/1‑149 (MQ=255)
          gggCAGGGACCAATGTGGGTGGTGGTTAACGCACACTAACCGCTGATTTACCCGGCGCAGTCTCTCCTGCGCCGGTGTATTAACCTATCTCCTGTAACGCGTGTCTCTGGCGTTCGACGATATTGGTCCACAAATTTTCTTTATCGTCa                                                                                                                                       >  1:935279/1‑149 (MQ=255)
          gggCAGGGACCAATGTGGGTGGTGGTTAACGCACACTAACCGCTGATTTACCCGGCGCAGTCTCTCCTGCGCCGGTGTATTAACCTATCTCCTGTAACGCGTGTCTCTGGCGTTCGACGATATTGGTCCACAAATTTTCTTTATCGTCa                                                                                                                                       <  2:506304/149‑1 (MQ=255)
          gggCAGGGACCAATGTGGGTGGTGGTTAACGCACACTAACCGCTGATTTACCCGGCGCAGTCTCTCCTGCGCCGGTGTATTAACCTATCTCCTGTAACGCGTGTCTCTGGCGTTCGACGATATTGGTCCACAAATTTTCTTTATCGTCa                                                                                                                                       <  2:382630/149‑1 (MQ=255)
          gggCAGGGACCAATGTGGGTGGTGGTTAACGCACACTAACCGCTGATTTACCCGGCGCAGTCTCTCCTGCGCCGGTGTATTAACCTATCTCCTGTAACGCGTGTCTCTGGCGTTCGACGATATTGGTCCACAAATTTTCTTTATCGTCa                                                                                                                                       >  2:2977/1‑149 (MQ=255)
           ggCAGGGACCAATGTGGGTGGTGGTTAACGCACACTAACCGCTGATTTACCCGGCGCAGTCTCTCCTGCGCCGGTGTATTAACCTATCTCCTGTAACGCGTGTCTCTGGCGTTCGACGATATTGGTCCACAAATTTTCTTTATCGTCAg                                                                                                                                      <  3:277482/149‑1 (MQ=255)
                ggACCAATGTGGGTGGTGGTTAACGCACACTAACCGCTGATTTACCCGGCGCAGTCTCTCCTGCGCCGGTGTATTAACCTATCTCCTGTAACGCGTGTCTCTGGCGTTCGACGATATTGGTCCACAAATTTTCTTTATCGTCAGTCCAt                                                                                                                                 >  1:665667/1‑149 (MQ=255)
                ggACCAATGTGGGTGGTGGTTAACGCACACTAACCGCTGATTTACCCGGCGCAGTCTCTCCTGCGCCGGTGTATTAACCTATCTCCTGTAACGCGTGTCTCTGGCGTTCGACGATATTGGTCCACAAATTTTCTTTATCGTCAGTCCAt                                                                                                                                 >  1:696048/1‑149 (MQ=255)
                   ccAATGTGGGTGGTGGTTAACGCACACTAACCGCTGATTTACCCGGCGCAGTCTCTCCTGCGCCGGTGTATTAACCTATCTCCTGTAACGCGTGTCTCTGGCGTTCGACGATATTGGTCCACAAATTTTCTTTATCGTCAGTCCATaaa                                                                                                                              >  2:914093/1‑149 (MQ=255)
                   ccAATGTGGGTGGTGGTTAACGCACACTAACCGCTGATTTACCCGGCGCAGTCTCTCCTGCGCCGGTGTATTAACCTATCTCCTGTAACGCGTGTCTCTGGCGTTCGACGATATTGGTCCACAAATTTTCTTTATCGTCAGTCCATaaa                                                                                                                              <  1:130307/149‑1 (MQ=255)
                    cAATGTGGGTGGTGGTTAACGCACACTAACCGCTGATTTACCCGGCGCAGTCTCTCCTGCGCCGGTGTATTAACCTATCTCCTGTAACGCGTGTCTCTGGCGTTCGACGATATTGGTCCACAAATTTTCTTTATCGTCAGTCCATAAAt                                                                                                                             <  3:247485/149‑1 (MQ=255)
                    cAATGTGGGTGGTGGTTAACGCACACTAACCGCTGATTTACCCGGCGCAGTCTCTCCTGCGCCGGTGTATTAACCTATCTCCTGTAACGCGTGTCTCTGGCGTTCGACGATATTGGTCCACAAATTTTCTTTATCGTCAGTCCATAAAt                                                                                                                             >  1:193221/1‑149 (MQ=255)
                            gtggtggtTAACGCACACTAACCGCTGATTTACCCGGCGCAGTCTCTCCTGCGCCGGTGTATTAACCTATCTCCTGTAACGCGTGTCTCTGGCGTTCGACGATATTGGTCCACAAATTTTCTTTATCGTCAGTCCATAAATTAATCAGc                                                                                                                     <  2:279508/149‑1 (MQ=255)
                              ggtggtTAACGCACACTAACCGCTGATTTACCCGGCGCAGTCTCTCCTGCGCCGGTGTATTAACCTATCTCCTGTAACGCGTGTCTCTGGCGTTCGACGATATTGGTCCACAAATTTTCTTTATCGTCAGTCCATAAATTAATCAGCaa                                                                                                                   <  1:633302/149‑1 (MQ=255)
                                 ggtTAACGCACACTAACCGCTGATTTACCCGGCGCAGTCTCTCCTGCGCCGGTGTATTAACCTATCTCCTGTAACGCGTGTCTCTGGCGTTCGACGATATTGGTCCACAAATTTTCTTTATCGTCAGTCCATAAATTAATCAGCAAGGc                                                                                                                <  2:883142/149‑1 (MQ=255)
                                  gtTAACGCACACTAACCGCTGATTTACCCGGCGCAGTCTCTCCTGCGCCGGTGTATTAACCTATCTCCTGTAACGCGTGTCTCTGGCGTTCGACGATATTGGTCCACAAATTTTCTTTATCGTCAGTCCATAAATTAATCAGCAAGGc                                                                                                                >  4:682333/1‑148 (MQ=255)
                                  gtTAACGCACACTAACCGCTGATTTACCCGGCGCAGTCTCTCCTGCGCCGGTGTATTAACCTATCTCCTGTAACGCGTGTCTCTGGCGTTCGACGATATTGGTCCACAAATTTTCTTTATCGTCAGTCCATAAATTAATCAGCAAGGc                                                                                                                <  3:682333/148‑1 (MQ=255)
                                  gtTAACGCACACTAACCGCTGATTTACCCGGCGCAGTCTCTCCTGCGCCGGTGTATTAACCTATCTCCTGTAACGCGTGTCTCTGGCGTTCGACGATATTGGTCCACAAATTTTCTTTATCGTCAGTCCATAAATTAATCAGCAAGGCa                                                                                                               >  2:621033/1‑149 (MQ=255)
                                     aaCACACACTAACCGCTGATTTACCCGGCGCAGTCTCTCCTGCGCCGGTGTATTAACCTATCTCCTGTAACGCGTGTCTCTGGCGTTCGACGATATTGGTTCACAAATTTTCTTTATCGTCAGTCCATAAATTAATCAGCAAGGCaaaa                                                                                                            >  1:751288/1‑149 (MQ=255)
                                       cGCACACTAACCGCTGATTTACCCGGCGCAGTCTCTCCTGCGCCGGTGTATTAACCTATCTCCTGTAACGCGTGTCTCTGGCGTTCGACGATATTGGTCCACAAATTTTCTTTATCGTCAGTCCATAAATTAATCAGCAAGGCaaaaaa                                                                                                          <  3:340217/149‑1 (MQ=255)
                                       cGCACACTAACCGCTGATTTACCCGGCGCAGTCTCTCCTGCGCCGGTGTATTAACCTATCTCCTGTAACGCGTGTCTCTGGCGTTCGACGATATTGGTCCACAAATTTTCTTTATCGTCAGTCCATAAATTAATCAGCAAGGCaaaaaa                                                                                                          <  2:665667/149‑1 (MQ=255)
                                            acTAACCGCTGATTTACCCGGCGCAGTCTCTCCTGCGCCGGTGTATTAACCTATCTCCTGTAACGCGTGTCTCTGGCGTTCGACGATATTGGTCCACAAATTTTCTTTATCGTCAGTCCATAAATTAATCAGCAAGGCAAAAAAGCGtt                                                                                                     <  4:804662/149‑1 (MQ=255)
                                            acTAACCGCTGATTTACCCGGCGCAGTCTCTCCTGCGCCGGTGTATTAACCTATCTCCTGTAACGCGTGTCTCTGGCGTTCGACGATATTGGTCCACAAATTTTCTTTATCGTCAGTCCATAAATTAATCAGCAAGGCAAAAAAGCGtt                                                                                                     <  2:193221/149‑1 (MQ=255)
                                              tAACCGCTGATTTACCCGGCGCAGTCTCTCCTGCGCCGGTGTATTAACCTATCTCCTGTAACGCGTGTCTCTGGCGTTCGACGATATTGGTCCACAAATTTTCTTTATCGTCAGTCCATAAATTAATCAGCAAGGCAAAAAAGCGTTct                                                                                                   >  2:484619/1‑149 (MQ=255)
                                              tAACCGCTGATTTACCCGGCGCAGTCTCTCCTGCGCCGGTGTATTAACCTATCTCCTGTAACGCGTGTCTCTGGCGTTCGACGATATTGGTCCACAAATTTTCTTTATCGTCAGTCCATAAATTAATCAGCAAGGCAAAAAAGCGTTct                                                                                                   >  4:420965/1‑149 (MQ=255)
                                                   gCTGATTTACCCGGCGCAGTCTCTCCTGCGCCGGTGTATTAACCTATCTCCTGTAACGCGTGTCTCTGGCGTTCGACGATATTGGTCCACAAATTTTCTTTATCGTCAGTCCATAAATTAATCAGCAAGGCAAAAAAGCGTTctgctgc                                                                                              >  1:799730/1‑149 (MQ=255)
                                                   gCTGATTTACCCGGCGCAGTCTCTCCTGCGCCGGTGTATTAACCTATCTCCTGTAACGCGTGTCTCTGGCGTTCGACGATATTGGTCCACAAATTTTCTTTATCGTCAGTCCATAAATTAATCAGCAAGGCAAAAAAGCGTTctgctgc                                                                                              >  1:256197/1‑149 (MQ=255)
                                                   gCTGATTTACCCGGCGCAGTCTCTCCTGCGCCGGTGTATTAACCTATCTCCTGTAACGCGTGTCTCTGGCGTTCGACGATATTGGTCCACAAATTTTCTTTATCGTCAGTCCATAAATTAATCAGCAAGGCAAAAAAGCGTTctgctgc                                                                                              >  2:820647/1‑149 (MQ=255)
                                                     tGATTTACCCGGCGCAGTCTCTCCTGCGCCGGTGTATTAACCTATCTCCTGTAACGCGTGTCTCTGGCGTTCGACGATATTGGTCCACAAATTTTCTTTATCGTCAGTCCATAAATTAATCAGCAAGGCAAAAAAGCGTTctgctgctg                                                                                            >  3:249362/1‑149 (MQ=255)
                                                        tttACCCGGCGCAGTCTCTCCTGCGCCGGTGTATTAACCTATCTCCTGTAACGCGTGTCTCTGGCGTTCGACGATATTGGTCCACAAATTTTCTTTATCGTCAGTCCATAAATTAATCAGCAAGGCAAAAAAGCGTTCTGCTGCTGgag                                                                                         >  2:104020/1‑149 (MQ=255)
                                                        tttACCCGGCGCAGTCTCTCCTGCGCCGGTGTATTAACCTATCTCCTGTAACGCGTGTCTCTGGCGTTCGACGATATTGGTCCACAAATTTTCTTTATCGTCAGTCCATAAATTAATCAGCAAGGCAAAAAAGCGTTCTGCTGCTGgag                                                                                         >  1:587184/1‑149 (MQ=255)
                                                             ccGGCGCAGTCTCTCCTGCGCCGGTGTATTAACCTATCTCCTGTAACGCGTGTCTCTGGCGTTCGACGATATTGGTCCACAAATTTTCTTTATCGTCAGTCCATAAATTAATCAGCAAGGCAAAAAAGCGTTCTGCTGCTGGAGAAAGt                                                                                    >  2:3443/1‑149 (MQ=255)
                                                                     gTCTCTCCTGCGCCGGTGTATTAACCTATCTCCTGTAACGCGTGTCTCTGGCGTTCGACGATATTGGTCCACAAATTTTCTTTATCGTCAGTCCATAAATTAATCAGCAAGGCAAAAAAGCGTTCTGCTGCTGGAGAAAGTACGGCATc                                                                            >  1:38019/1‑149 (MQ=255)
                                                                       ctctcCTGCGCCGGTGTATTAACCTATCTCCTGTAACGCGTGTCTCTGGCGTTCGACGATATTGGTCCACAAATTTTCTTTATCGTCAGTCCATAAATTAATCAGCAAGGCAAAAAAGCGTTCTGCTGCTGGAGAAAGTACGGCATCtt                                                                          >  4:309032/1‑149 (MQ=255)
                                                                        tctcCTGCGCCGGTGTATTAACCTATCTCCTGTAACGCGTGTCTCTGGCGTTCGACGATATTGGTCCACAAATTTTCTTTATCGTCAGTCCATAAATTAATCAGCAAGGCAAAAAAGCGTTCTGCTGCTGGAGAAAGTACGGCATCttt                                                                         >  4:366549/1‑149 (MQ=255)
                                                                        tctcCTGCGCCGGTGTATTAACCTATCTCCTGTAACGCGTGTCTCTGGCGTTCGACGATATTGGTCCACAAATTTTCTTTATCGTCAGTCCATAAATTAATCAGCAAGGCAAAAAAGCGTTCTGCTGCTGGAGAAAGTACGGCATCttt                                                                         >  4:395340/1‑149 (MQ=255)
                                                                          tcCTGCGCCGGTGTATTAACCTATCTCCTGTAACGCGTGTCTCTGGCGTTCGACGATATTGGTCCACAAATTTTCTTTATCGTCAGTCCATAAATTAATCAGCAAGGCAAAAAAGCGTTCTGCTGCTGGAGAAAGTACGGCATCTTTAc                                                                       <  2:587184/149‑1 (MQ=255)
                                                                           ccTGCGCCGGTGTATTAACCTATCTCCTGTAACGCGTGTCTCTGGCGTTCGACGATATTGGTCCACAAATTTTCTTTATCGTCAGTCCATAAATTAATCAGCAAGGCAAAAAAGCGTTCTGCTGCTGGAGAAAGTACGGCATCTTTACg                                                                      >  1:324862/1‑149 (MQ=255)
                                                                           ccTGCGCCGGTGTATTAACCTATCTCCTGTAACGCGTGTCTCTGGCGTTCGACGATATTGGTCCACAAATTTTCTTTATCGTCAGTCCATAAATTAATCAGCAAGGCAAAAAAGCGTTCTGCTGCTGGAGAAAGTACGGCATCTTTACg                                                                      <  4:377967/149‑1 (MQ=255)
                                                                            cTGCGCCGGTGTATTAACCTATCTCCTGTAACGCGTGTCTCTGGCGTTCGACGATATTGGTCCACAAATTTTCTTTATCGTCAGTCCATAAATTAATCAGCAAGGCAAAAAAGCGTTCTGCTGCTGGAGAAAGTACGGCATCTTTACgg                                                                     <  3:366549/149‑1 (MQ=255)
                                                                             tGCGCCGGTGTATTAACCTATCTCCTGTAACGCGTGTCTCTGGCGTTCGACGATATTGGTCCACAAATTTTCTTTATCGTCAGTCCATAAATTAATCAGCAAGGCAAAAAAGCGTTCTGCTGCTGGAGAAAGTACGGCATCTTTACGGc                                                                    >  4:28708/1‑149 (MQ=255)
                                                                              gcgcCGGTGTATTAACCTATCTCCTGTAACGCGTGTCTCTGGCGTTCGACGATATTGGTCCACAAATTTTCTTTATCGTCAGTCCATAAATTAATCAGCAAGGCAAAAAAGCGTTCTGCTGCTGGAGAAAGTACGGCATCTTTACGGCg                                                                   <  2:30734/149‑1 (MQ=255)
                                                                                gcCGGTGTATTAACCTATCTCCTGTAACGCGTGTCTCTGGCGTTCGACGATATTGGTCCACAAATTTTCTTTATCGTCAGTCCATAAATTAATCAGCAAGGCAAAAAAGCGTTc                                                                                                    >  1:258555/1‑114 (MQ=255)
                                                                                gcCGGTGTATTAACCTATCTCCTGTAACGCGTGTCTCTGGCGTTCGACGATATTGGTCCACAAATTTTCTTTATCGTCAGTCCATAAATTAATCAGCAAGGCAAAAAAGCGTTc                                                                                                    <  2:258555/114‑1 (MQ=255)
                                                                                    gtgtATTAACCTATCTCCTGTAACGCGTGTCTCTGGCGTTCGACGATATTGGTCCACAAATTTTCTTTATCGTCAGTCCATAAATTAATCAGCAAgg                                                                                                                 >  2:572450/1‑97 (MQ=255)
                                                                                     tgtATTAACCTATCTCCTGTAACGCGTGTCTCTGGCGTTCGACGATATTGGTCCACAAATTTTCTTTATCGTCAGTCCATAAATTAATCAGCAAGGCAAAAAAGCGTTCTGCTGCTGGAGAAAGTACGGCATCTTTACGGCGAattatt                                                            <  3:210744/149‑1 (MQ=255)
                                                                                      gtATTAACCTATCTCCTGTAACGCGTGTCTCTGGCGTTCGACGATATTGGTCCACAAATTTTCTTTATCGTCAGTCCATAAATTAATCAGCAAGGCAAAAAAGCGTTCTGCTGCTGGAGAAAGTACGGCATCTTTACGGCGAATTATTc                                                           <  3:691785/149‑1 (MQ=255)
                                                                                       tATTAACTTATCTCCTGTAACGCGTGTCTCTGGCGTTCGACGATATTGGTCCACAAATTTTCTTTATCGTCAGTCCATAAATTAATCAGCAAGGCAAAAAAGCGTTCTGCTGCTGGAGAAAGTACGGCATCTTTACGGCGAATTATTcc                                                          <  4:249362/149‑1 (MQ=255)
                                                                                        aTTAACCTATCTCCTGTAACGCGTGTCTCTGGCGTTCGACGATATTGGTCCACAAATTTTCTTTATCGTCAGTCCATAAATTAATCAGCAAGGCAAAAAAGCGTTCTGCTGCTGGAGAAAGTACGGCATCTTTACGGCGAATTATTccc                                                         >  3:908483/1‑149 (MQ=255)
                                                                                        aTTAACCTATCTCCTGTAACGCGTGTCTCTGGCGTTCGACGATATTGGTCCACAAATTTTCTTTATCGTCAGTCCATAAATTAATCAGCAAGGCAAAAAAGCGTTCTGCTGCTCGAGAAAGTACGGTACCTTTACGGCGAATTACTccc                                                         <  2:533183/149‑1 (MQ=255)
                                                                                         ttAACCTATCTCCTGTAACGCGTGTCTCTGGCGTTCGACGATATTGGTCCACAAATTTTCTTTATCGTCAGTCCATAAATTAATCAGCAAGGCAAAAAAGCGTTCTGCTGCTGGAGAAAGTACGGCATCTTTACGGCGAATTATTCCCa                                                        >  1:727087/1‑149 (MQ=255)
                                                                                         ttAACCTATCTCCGTAACGCGTGTCTCTGGCGTTCGACGATATTGGTCCACAAATTTTCTTTATCGTCAGTCCATAAATTAATCAGCAAGGCAAAAAAGCGTTCTGCTGCTGGAGAAAGTACGGCATCTTTACGGCGAATTATTCCCaa                                                       <  1:486048/149‑1 (MQ=255)
                                                                                           aaCCTATCTCCTGTAACGCGTGTCTCTGGCGTTCGACGATATTGGTCCACAAATTTTCTTTATCGTCAGTCCATAAATTAATCAGCAAGGCAAAAAAGCGTTCTGCTGCTGGAGAAAGTACGGCATCTTTACGGCGAATTATTCCCAAt                                                      <  1:484619/149‑1 (MQ=255)
                                                                                            aCCTATCTCCTGTAACGCGTGTCTCTGGCGTTCGACGATATTGGTCCACAAATTTTCTTTATCGTCAGTCCATAAATTAATCAGCAAGGCAAAAAAGCGTTCTGCTGCTGGAGAAAGTACGGCATCTTTACGGCGAATTATTCCCAATg                                                     >  4:589085/1‑149 (MQ=255)
                                                                                              cTATCTCCTGTAACGCGTGTCTCTGGCGTTCGACGATATTGGTCCACAAATTTTCTTTATCGTCAGTCCATAAATTAATCAGCAAGGCAAAAAAGCGTTCTGCTGCTGGAGAAAGTACGGCATCTTTACGGCGAATTATTCCCAATGtt                                                   <  1:820647/149‑1 (MQ=255)
                                                                                              cTATCTCCTGTAACGCGTGTCTCTGGCGTTCGACGATATTGGTCCACAAATTTTCTTTATCGTCAGTCCATAAATTAATCAGCAAGGCAAAAAAGCGTTCTGCTGCTGGAGAAAGTACGGCATCTTTACGGCGAATTATTCCCAATGtt                                                   >  4:252638/1‑149 (MQ=255)
                                                                                               tATCTCCTGTAACGCGTGTCTCTGGCGTTCGACGATATTGGTCCACAAATTTTCTTTATCGTCAGTCCATAAATTAATCAGCAAGGCAAAAAAGCGTTCTGCTGCTGGAGAAAGTACGGCATCTTTACGGCGAATTATTCCCAATGTTc                                                  <  4:305300/149‑1 (MQ=255)
                                                                                               tATCTCCTGTAACGCGTGTCTCTGGCGTTCGACGATATTGGTCCACAAATTTTCTTTATCGTCAGTCCATAAATTAATCAGCAAGGCAAAAAAGCGTTCTGCTGCTGGAGAAAGTACGGCATCTTTACGGCGAATTATTCCCAATGTTc                                                  >  3:46430/1‑149 (MQ=255)
                                                                                               tATCTCCTGTAACGCGTGTCTCTGGCGTTCGACGATATTGGTCCACAAATTTTCTTTATCGTCAGTCCATAAATTAATCAGCAAGGCAAAAAAGCGTTCTGCTGCTGGAGAAAGTACGGCATCTTTACGGCGAATTATTCCCAATGTTc                                                  >  1:262927/1‑149 (MQ=255)
                                                                                                 tctcGTGTAACGCGTGTCTCTGGCGTTCGACGATATTGGTCCACAAATTTTCTTTATCGTCAGTCCATAAATTAATCAGCAAGGCAAAAAAGCGTTCTGCTGCTGGAGAAAGTACGGCATCTTTACGGCGAATTATTCCCAATGTTCGa                                                >  4:50659/1‑149 (MQ=255)
                                                                                                  ctcCTGTAACGCGTGTCTCTGGCGTTCGACGATATTGGTCCACAAATTTTCTTTATCGTCAGTCCATAAATTAATCAGCAAGGCAAAAAAGCGTTCTGCTGCTGGAGAAAGTACGGCATCTTTACGGCGAATTATTCCCAATGTTCGAc                                               <  2:855297/149‑1 (MQ=255)
                                                                                                  ctcCTGTAACGCGTGTCTCTGGCGTTCGACGATATTGGTCCACAAATTTTCTTTATCGTCAGTCCATAAATTAATCAGCAAGGCAAAAAAGCGTTCTGCTGCTGGAGAAAGTACGGCATCTTTACGGCGAATTATTCCCAATGTTCGAc                                               >  2:712263/1‑149 (MQ=255)
                                                                                                   tcCTGTAACGCGTGTCTCTGGCGTTCGACGATATTGGTCCACAAATTTTCTTTATCGTCAGTCCATAAATTAATCAGCAAGGCAAAAAAGCGTTCTGCTGCTGGAGAAAGTACGGCATCTTTACGGCGAATTATTCCCAATGTTCGACg                                              >  1:672109/1‑149 (MQ=255)
                                                                                                   tcCTGTAACGCGTGTCTCTGGCGTTCGACGATATTGGTCCACAAATTTTCTTTATCGTCAGTCCATAAATTAATCAGCAAGGCAAAAAAGCGTTCTGCTGCTGGAGAAAGTACGGCATCTTTACGGCGAATTATTCCCAATGTTCGACg                                              >  1:520974/1‑149 (MQ=255)
                                                                                                      tGTAACGCGTGTCTCTGGCGTTCGACGATATTGGTCCACAAATTTTCTTTATCGTCAGTCCATAAATTAATCAGCAAGGCAAAAAAGCGTTCTGCTGCTGGAGAAAGTACGGCATCTTTACGGCGAATTATTCCCAATGTTCGACGtat                                           >  4:56300/1‑149 (MQ=255)
                                                                                                         aaCGCGTGTCTCTGGCGTTCGACGATATTGGTCCACAAATTTTCTTTATCGTCAGTCCATAAATTAATCAGCAAGGCAAAAAAGCGTTCTGCTGCTGGAGAAAGTACGGCATCTTTACGGCGAATTATTCCCAATGTTCGACGTATAAc                                        <  3:230916/149‑1 (MQ=255)
                                                                                                          aCGCGTGTCTCTGGCGTTCGACGATATTGGTCCACAAATTTTCTTTATCGTCAGTCCATAAATTAATCAGCAAGGCAAAAAAGCGTTCTGCTGCTGGAGAAAGTACGGCATCTTTACGGCGAATTATTCCCAATGTTCGACGTATAACg                                       <  3:387612/149‑1 (MQ=255)
                                                                                                              gtgtCTCTGGCGTTCGACGATATTGGTCCACAAATTTTCTTTATCGTCAGTCCATAAATTAATCAGCAAGGCAAAAAAGCGTTCTGCTGCTGGAGAAAGTACGGCATCTTTACGGCGAATTATTCCCAATGTTCGACGTATAACGGGtt                                   >  4:231203/1‑149 (MQ=255)
                                                                                                                   tctGGCGTTCGACGATATTGGTCCACAAATTTTCTTTATCGTCAGTCCATAAATTAATCAGCAAGGCAAAAAAGCGTTCTGCTGCTGGAGAAAGTACGGCATCTTTACGGCGAATTATTCCCAATGTTCGACGTATAACGGGTTccacc                              >  2:402898/1‑149 (MQ=255)
                                                                                                                      ggCGTTCGACGATATTGGTCCACAAATTTTCTTTATCGTCAGTCCATAAATTAATCAGCAAg                                                                                                                  <  3:253089/62‑1 (MQ=255)
                                                                                                                      ggCGTTCGACGATATTGGTCCACAAATTTTCTTTATCGTCAGTCCATAAATTAATCAGCAAg                                                                                                                  >  4:253089/1‑62 (MQ=255)
                                                                                                                      ggCGTTCGACGATATTGGTCCACAAATTTTCTTTATCGTCAGTCCATAAATTAATCAGCAAGGCAAAAAAGCGTTCTGCTGCTGGAGAAAGTACGGCATCTTTACGGCGAATTATTCCCAATGTTCGACGTATAACGGGTTCCACCAAt                           <  2:696048/149‑1 (MQ=255)
                                                                                                                       gCGTTCGACGATATTGGTCCACAAATTTTCTTTATCGTCAGTCCATAAATTAATCAGCAAGGCAAAAAAGCGTTCTGCTGCTGGAGAAAGTACGGCATCTTTACGGCGAATTATTCCCAATGTTCGACGTATAACGGGTTCCACCAATg                          >  4:431169/1‑149 (MQ=255)
                                                                                                                       gCGTTCGACGATATTGGTCCACAAATTTTCTTTATCGTCAGTCCATAAATTAATCAGCAAGGCAAAAAAGCGTTCTGCTGCTGGAGAAAGTACGGCATCTTTACGGCGAATTATTCCCAATGTTCGACGTATAACGGGTTCCACCAATg                          >  4:389102/1‑149 (MQ=255)
                                                                                                                             gacgaTATTGGTCCACAAATTTTCTTTATCGTCAGTCCATAAATTAATCAGCAAGGCAAAAAAGCGTTCTGCTGCTGGAGAAAGTACGGCATCTTTACGGCGAATTATTCCCAATGTTCGACGTATAACGGGTTCCACCAATGGGATAc                    <  3:252638/149‑1 (MQ=255)
                                                                                                                             gacgaTATTGGTCCACAAATTTTCTTTATCGTCAGTCCATAAATTAATCAGCAAGGCAAAAAAGCGTTCTGCTGCTGGAGAAAGTACGGCATCTTTACGGCGAATTATTCCCAATGTTCGACGTATAACGGGTTCCACCAATGGGATAc                    <  3:414659/149‑1 (MQ=255)
                                                                                                                             gacgaTATTGGTCCACAAATTTTCTTTATCGTCAGTCCATAAATTAATCAGCAAGGCAAAAAAGCGTTCTGCTGCTGGAGAAAGTACGGCATCTTTACGGCGAATTATTCCCAATGTTCGACGTATAACGGGTTCCACCAATGGGATAc                    >  4:160672/1‑149 (MQ=255)
                                                                                                                              acgaTATTGGTCCACAAATTTTCTTTATCGTCAGTCCATAAATTAATCAGCAAGGCAAAAAAGCGTTCTGCTGCTGGAGAAAGTACGGCATCTTTACGGCGAATTATTCCCAATGTTCGACGTATAACGGGTTCCACCAATGGGATAcc                   <  1:2977/149‑1 (MQ=255)
                                                                                                                                   tttGGTCCACAAATTTTCTTTATCGTCAGTCCATAAATTAATCAGCAAGGCAAAAAAGCGTTCTGCTGCTAGAGAAAGTACGGCATCTTTACGGCGAATTATTCCCAATGTTCGACGTATAACGGGTTCCACCAATGGGATACCaataa              <  1:402898/148‑1 (MQ=255)
                                                                                                                                       gTCCACAAATTTTCTTTATCGTCAGTCCATAAATTAATCAGCAAGGCAAAAAAGCGTTCTGCTGCTGGAGAAAGTACGGCATCTTTACGGCGAATTATTCCCAATGTTCGACGTATAACGGGTTCCACCAATGGGATACCAATAATCga          >  4:597222/1‑149 (MQ=255)
                                                                                                                                        tCCACAAATTTTCTTTATCGTCAGTCCATAAATTAATCAGCAAGGCAAAAAAGCGTTCTGCTGCTGGAGAAAGTACGGCATCTTTACGGCGAATTATTCCCAATGTTCGACGTATAACGGGTTCCACCAATGGGATACCAATAATCgaa         <  3:5472/149‑1 (MQ=255)
                                                                                                                                         ccACAAATTTTCTTTATCGTCAGTCCATAAATTAATCAGCAAGGCAAAAAAGCGTTCTGCTGCTGGAGAAAGTACGGCATCTTTACGGCGAATTATTCCCAATGTTCGACGTATAACGGGTTCCACCAATGGGATACCAATAATCgaag        >  3:598716/1‑149 (MQ=255)
                                                                                                                                          cacaAATTTTCTTTATCGTCAGTCCATAAATTAATCAGCAAGGCAAAAAAGCGTTCTGCTGCTGGAGAAAGTa                                                                                   >  3:483498/1‑73 (MQ=255)
                                                                                                                                          cacaAATTTTCTTTATCGTCAGTCCATAAATTAATCAGCAAGGCAAAAAAGCGTTCTGCTGCTGGAGAAAGTa                                                                                   <  4:483498/73‑1 (MQ=255)
                                                                                                                                           acaAATTTTCTTTATCGTCAGTCCATAAATTAATCAGCAAGGCAAAAAAGCGTTCTGCTGCTGGAGAAAGTACGGCATCTTTACGGCGAATTATTCCCAATGTTCGACGTATAACGGGTTCCACCAATGGGATACCAATAATCgaagaa      >  2:77732/1‑149 (MQ=255)
                                                                                                                                           acaAATTTTCTTTATCGTCAGTCCATAAATTAATCAGCAAGGCAAAAAAGCGTTCTGCTGCTGGAGAAAGTACGGCATCTTTACGGCGAATTATTCCCAATGTTCGACGTATAACGGGTTCCACCAATGGGATACCAATAATCgaagaa      >  1:200362/1‑149 (MQ=255)
                                                                                                                                            caAATTTTCTTTATCGTCAGTCCATAAATTAATCAGCAAGGCAAAAAAGCGTTCTGCTGCTGGAGAAAGTACGGCATCTTTACGGCGAATTATTCCCAATGTTCGACGTATAACGGGTTCCACCAAt                           >  4:31950/1‑127 (MQ=255)
                                                                                                                                            caAATTTTCTTTATCGTCAGTCCATAAATTAATCAGCAAGGCAAAAAAGCGTTCTGCTGCTGGAGAAAGTACGGCATCTTTACGGCGAATTATTCCCAATGTTCGACGTATAACGGGTTCCACCAAt                           <  3:31950/127‑1 (MQ=255)
                                                                                                                                              aaTTTTCTTTATCGTCAGTCCATAAATTAATCAGCAAGGCAAAAAAGCGTTCTGCTGCTGGAGAAAGTACGGCATCTTTACGGCGAATTATTCCCAATGTTCGACGTATAACGTGTTCCACCAATGGGATACCAATAATCGAAGAATAg   >  1:583356/1‑149 (MQ=255)
                                                                                                                                              aaTTTTCTTTATCGTCAGTCCATAAATTAATCAGCAAGGCAAAAAAGCGTTCTGCTGCTGGAGAAAGTACGGCATCTTTACGGCGAATTATTCCCAATGTTCGACGTATAACGGGTTCCACCAATGGGATACCAATAATCGAAGAATAg   <  1:621033/149‑1 (MQ=255)
                                                                                                                                               aTTTTCTTTATCGTCAGTCCATAAATTAATCAGCAAGGCAAAAAAGCGTTCTGCTGCTGGAGAAAGTACGGCATCTTTACGGCGAATTATTCCCAATGTTCGACGTATAACGGGTTCCACCAATGGGATACCAATAATCGAAGAATAgg  <  3:395340/149‑1 (MQ=255)
                                                                                                                                               aTTTTCTTTATCGTCAGTCCATAAATTAATCAGCAAGGCAAAAAAGCGTTCTGCTGCTGGAGAAAGTACGGCATCTTTACGGCGAATTATTCCCAATGTTCGACGTATAACGGGTTCCACCAATGGGATACCAATAATCGAAGAATAgg  <  4:737381/149‑1 (MQ=255)
                                                                                                                                               aTTTTCTTTATCGTCAGTCCATAAATTAATCAGCAAGGCAAAAAAGCGTTCTGCTGCTGGAGAAAGTACGGCATCTTTACGGCGAATTATTCCCAATGTTCGACGTATAACGGGTTCCACCAATGGGATACCAATAATCGAAGAATAgg  <  3:589085/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
CTATGCGGTCGGGCAGGGACCAATGTGGGTGGTGGTTAACGCACACTAACCGCTGATTTACCCGGCGCAGTCTCTCCTGCGCCGGTGTATTAACCTATCTCCTGTAACGCGTGTCTCTGGCGTTCGACGATATTGGTCCACAAATTGTCTTTATCGTCAGTCCATAAATTAATCAGCAAGGCAAAAAAGCGTTCTGCTGCTGGAGAAAGTACGGCATCTTTACGGCGAATTATTCCCAATGTTCGACGTATAACGGGTTCCACCAATGGGATACCAATAATCGAAGAATAGG  >  NC_000913/799533‑799824

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: