Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 899,656 T→C 9.6% intergenic (+11/+188) rlmC → / ← artJ 23S rRNA m(5)U747 methyltransferase, SAM‑dependent/arginine ABC transporter periplasmic binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913899,6560TC 9.6% 316.4 / 19.5 104intergenic (+11/+188)rlmC/artJ23S rRNA m(5)U747 methyltransferase, SAM‑dependent/arginine ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base T (33/61);  new base C (3/7);  total (36/68)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.35e-01

Only 100 of 105 total aligned reads displayed.

TCCAGCTGTAACGCCCAAACTATGGCGAAAGATATCCGCGAACTGCCTGGGTTTCGTATTGAACGGGTGCAGCTTTTCGATATGTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGCCATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTACC  >  NC_000913/899511‑899802
                                                                                                                                                 |                                                                                                                                                  
tCCAGCTGTAACGCCCAAACTATGGCGAAAGATATCCGCGAACTGCCTGGGTTTCGTATTGAACGGGTGCAGCTTTTCGATATGTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCAtgtg                                                                                                                                                 >  1:369440/1‑149 (MQ=255)
 ccAGCTGTAACGCCCAAACTATGGCGAAAGATATCCGCGAACTGCCTGGGTTTCGTATTGAACGGGTGCAGCTTTTCGATATGTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGc                                                                                                                                                >  3:426980/1‑149 (MQ=255)
    gCTGTAACGCCCAAACTATGGCGAAAGATATCCGCGAACTGCCTGGGTTTCGTATTGAACGGGTGCAGCTTTTCGATATGTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGc                                                                                                                                             <  3:497523/149‑1 (MQ=255)
      tGTAACGCCCAAACTATGGCGAAAGATATCCGCGAACTGCCTGGGTTTCGTATTGAACGGGTGCAGCTTTTCGATATGTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCACATGCGGCtt                                                                                                                                           <  2:626790/149‑1 (MQ=255)
       gTAACGCCCAAACTATGGCGAAAGATATCCGCGAACTGCCTGGGTTTCGTATTGAACGGGTGCAGCTTTTCGATATGTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCACATGCGGCttt                                                                                                                                          <  1:395914/149‑2 (MQ=255)
       gTAACGCCCAAACTATGGCGAAAGATATCCGCGAACTGCCTGGGTTTCGTATTGAACGGGTGCAGCTTTTCGATATGTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCACATGCGGCttt                                                                                                                                          <  3:841172/149‑2 (MQ=255)
       gTAACGCCCAAACTATGGCGAAAGATATCCGCGAACTGCCTGGGTTTCGTATTGAACGGGTGCAGCTTTTCGATATGTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCACATGCGGCttt                                                                                                                                          <  1:261647/149‑2 (MQ=255)
        tAACGCCCAAACTATGGCGAAAGATATCCGCGAACTGCCTGGGTTTCGTATTGAACGGGTGCAGCTTTTCGATATGTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCa                                                                                                                                         <  1:547735/149‑1 (MQ=255)
          aCGCCCAAACTATGGCGAAAGATATCCGCGAACTGCCTGGGTTTCGTATTGAACGGGTGCAGCTTTTCGATATGTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGa                                                                                                                                       <  3:536229/149‑1 (MQ=255)
             cccAAACTATGGCGAAAGATATCCGCGAACTGCCTGGGTTTCGTATTGAACGGGTGCAGCTTTTCGATATGTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAg                                                                                                                                        <  1:439469/145‑1 (MQ=255)
              ccAAACTATGGCGAAAGTTATCCGCGAACTGCCTGGGTTTCGTATTGAACGGGTGCAGCTTTTCGATATGTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGc                                                                                                                                   <  3:763335/149‑1 (MQ=255)
              ccAAACTATGGCGAAAGATATCCGCGAACTGCCTGGGTTTCGTATTGAACGGGTGCAGCTTTTCGATATGTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGc                                                                                                                                   <  2:208709/149‑1 (MQ=255)
              ccAAACTATGGCGAAAGATATCCGCGAACTGCCTGGGTTTCGTATTGAACGGGTGCAGCTTTTCGATATGTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGc                                                                                                                                   >  2:392344/1‑149 (MQ=255)
               cAAACTATGGCGAAAGATATCCGCGAACTGCCTGGGTTTCGTATTGAACGGGTGCAGCTTTTCGATATGTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCt                                                                                                                                  <  1:299673/149‑1 (MQ=255)
               cAAACTATGGCGAAAGATATCCGCGAACTGCCTGGGTTTCGTATTGAACGGGTGCAGCTTTTCGATATGTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCt                                                                                                                                  <  1:248743/149‑1 (MQ=255)
               cAAACTATGGCGAAAGATATCCGCGAACTGCCTGGGTTTCGTATTGAACGGGTGCAGCTTTTCGATATGTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCt                                                                                                                                  <  2:554337/149‑1 (MQ=255)
               cAAACTATGGCGAAAGATATCCGCGAACTGCCTGGGTTTCGTATTGAACGGGTGCAGCTTTTCGATATGTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCt                                                                                                                                  <  1:544753/149‑1 (MQ=255)
                 aaCTATGGCGAAAGATATCCGCGAACTGCCTGGGTTTCGTATTGAACGGGTGCAGCTTTTCGATATGTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGa                                                                                                                                >  3:404840/1‑149 (MQ=255)
                 aaCTATGGCGAAAGATATCCGCGAACTGCCTGGGTTTCGTATTGAACGGGTGCAGCTTTTCGATATGTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGa                                                                                                                                <  2:456747/149‑1 (MQ=255)
                  aCTATGGCGAAAGATATCCGCGAACTGCCTGGGTTTCGTATTGAACGGGTGCAGCTTTTCGATATGTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGAc                                                                                                                               <  1:407062/149‑1 (MQ=255)
                       ggCGAAAGATATCCGCGAACTGCCTGGGTTTCGTATTGAACGGGTGCAGCTTTTCGATATGTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGt                                                                                                                          <  4:777124/149‑1 (MQ=255)
                           aaaGATATCCGCGAACTGCCTGGGTTTCGTATTGAACGGGTGCAGCTTTTCGATATGTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTgcgc                                                                                                                      <  4:779326/149‑1 (MQ=255)
                           aaaGATATCCGCGAACTGCCTGGGTTTCGTATTGAACGGGTGCAGCTTTTCGATATGTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTgcgc                                                                                                                      >  1:197957/1‑149 (MQ=255)
                                 atCCGCGAACTGCCTGGGTTTCGTATTGAACGGGTGCAGCTTTTCGATATGTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCgttgtt                                                                                                                <  3:757079/149‑1 (MQ=255)
                                   cagcgAACTGCCTGGGTTTCGTATTGAACGGGTGCAGCTTTTCGATATGTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTa                                                                                                              <  3:94260/147‑1 (MQ=255)
                                    cgcgAACTGCCTGGGTTTCGTATTGAACGGGTGCAGCTTTTCGATATGTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTAt                                                                                                             >  2:408061/1‑149 (MQ=255)
                                    cgcgAACTGCCTGGGTTTCGTATTGAACGGGTGCAGCTTTTCGATATGTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTAt                                                                                                             >  3:532535/1‑149 (MQ=255)
                                           tGCCTGGGTTTCGTATTGAACGGGTGCAGCTTTTCGATATGTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGAt                                                                                                      >  4:875414/1‑149 (MQ=255)
                                             ccTGGGTTTCGTATTGAACGGGTGCAGCTTTTCGATATGTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATgc                                                                                                    >  4:445805/1‑149 (MQ=255)
                                             ccTGGGTTTCGTATTGAACGGGTGCAGCTTTTCGATATGTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATgc                                                                                                    <  4:660189/149‑1 (MQ=255)
                                              cTGGGTTTCGTATTGAACGGGTGCAGCTTTTCGATATGTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATgcg                                                                                                   <  1:392344/149‑1 (MQ=255)
                                              cTGGGTTTCGTATTGAACGGGTGCAGCTTTTCGATATGTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATgcg                                                                                                   >  3:858259/1‑149 (MQ=255)
                                              cTGGGTTTAGTATTGAACGGGTGCAGCTTTTCGATATGTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATgcg                                                                                                   <  2:806154/149‑1 (MQ=255)
                                                      cGTATTGAACGGGTGCAGCTTTTCGATATGTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAAc                                                                                           <  2:816741/149‑1 (MQ=255)
                                                      cGTATTGAACGGGTGCAGCTTTTCGATATGTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAAc                                                                                           <  2:369440/149‑1 (MQ=255)
                                                      cGTATTGAACGGGTGCAGCTTTTCGATATGTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAAc                                                                                           <  1:837739/149‑1 (MQ=255)
                                                         aTTGAACGGGTGCAGCTTTTCGATATGTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTACGCCGCATCCGGCATAAAcaac                                                                                        <  3:864463/149‑4 (MQ=255)
                                                          ttGAACGGGTGCAGCTTTTCGATATGTTCCCGCATACCGCGCACTATGAAGTGTTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCt                                                                                       >  4:499890/1‑149 (MQ=255)
                                                          ttGAACGGGTGCAGCTTTTCGATATGTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCt                                                                                       >  4:827239/1‑149 (MQ=255)
                                                           tGAACGGGTGCAGCTTTTCGATATGTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCACATGCGGCttt                                                                                                                                          >  3:891478/1‑96 (MQ=255)
                                                           tGAACGGGTGCAGCTTTTCGATATGTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCACATGCGGCttt                                                                                                                                          <  4:891478/97‑2 (MQ=255)
                                                              aCGGGTGCAGCTTTTCGATATGTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATc                                                                                   <  3:715201/149‑1 (MQ=255)
                                                              aCGGGTGCAGCTTTTCGATATGTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATc                                                                                   <  1:419450/149‑1 (MQ=255)
                                                                gggTGCAGCTTTTCGATATGTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCt                                                                                                                                            >  4:407386/1‑90 (MQ=255)
                                                                gggTGCAGCTTTTCGATATGTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCt                                                                                                                                            <  3:407386/90‑1 (MQ=255)
                                                                  gTGCAGCTTTTCGATATGTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGc                                                                               <  1:370723/149‑1 (MQ=255)
                                                                   tGCAGCTTTTCGATATGTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGcc                                                                              <  1:170499/149‑1 (MQ=255)
                                                                   tGCAGCTTTTCGATATGTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGcc                                                                              >  3:4322/1‑149 (MQ=255)
                                                                              gATATGTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGt                                                                   <  2:914034/149‑1 (MQ=255)
                                                                              gATATGTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGt                                                                   <  2:197957/149‑1 (MQ=255)
                                                                                 atGTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTc                                                                                                                                          >  2:924694/1‑75 (MQ=255)
                                                                                 atGTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTc                                                                                                                                          <  1:924694/75‑1 (MQ=255)
                                                                                   gTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCaaa                                                              <  3:288303/149‑1 (MQ=255)
                                                                                   gTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCaaa                                                              <  1:438510/149‑1 (MQ=255)
                                                                                      cccGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTc                                                           >  1:636790/1‑149 (MQ=255)
                                                                                       ccGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCa                                                          >  2:19800/1‑149 (MQ=255)
                                                                                        cGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCaa                                                         <  2:207818/149‑1 (MQ=255)
                                                                                             aCCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACAt                                                    <  1:254898/149‑1 (MQ=255)
                                                                                               cgcgCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTg                                                                                                                                 <  2:235232/70‑1 (MQ=255)
                                                                                               cgcgCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTg                                                                                                                                 >  1:235232/1‑70 (MQ=255)
                                                                                               cgcgCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACATTg                                                  >  4:80517/1‑149 (MQ=255)
                                                                                                  gcACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCAt                                               <  4:4322/149‑1 (MQ=255)
                                                                                                   cACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATg                                              <  2:668051/149‑1 (MQ=255)
                                                                                                    aCTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTgc                                                                                                                        >  3:517602/1‑74 (MQ=255)
                                                                                                    aCTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTgc                                                                                                                        <  4:517602/74‑1 (MQ=255)
                                                                                                    aCTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATgg                                             <  1:849399/149‑1 (MQ=255)
                                                                                                     cTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATggg                                            <  1:233865/149‑1 (MQ=255)
                                                                                                     cTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATggg                                            >  2:174650/1‑149 (MQ=255)
                                                                                                      tATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATc                                                                                   <  4:706423/109‑1 (MQ=255)
                                                                                                      tATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATc                                                                                   >  3:706423/1‑109 (MQ=255)
                                                                                                      tATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGc                                           >  3:459833/1‑149 (MQ=255)
                                                                                                      tATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGc                                           >  4:407055/1‑149 (MQ=255)
                                                                                                             tGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGCCATGTAg                                    <  3:445805/149‑1 (MQ=255)
                                                                                                                 gACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGCCATGTAGGCCt                                <  4:843795/149‑1 (MQ=255)
                                                                                                                 caCGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGCCATGTAGGCCt                                <  3:80517/148‑1 (MQ=255)
                                                                                                                   cGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGCCATGTAGGCCTGa                              <  4:459833/149‑1 (MQ=255)
                                                                                                                      tgctgGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGTAAATTCAATACATTGCATGGGCCATg                                       <  4:319522/137‑1 (MQ=255)
                                                                                                                      tgctgGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGCCATg                                       >  3:319522/1‑137 (MQ=255)
                                                                                                                         tgGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCtt                                                                                      <  3:716195/87‑1 (MQ=255)
                                                                                                                         tgGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCtt                                                                                      >  4:716195/1‑87 (MQ=255)
                                                                                                                          ggTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGCCATGTAGGCCTGATAAGCGt                       <  3:841150/149‑1 (MQ=255)
                                                                                                                             gAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTCTCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGCCATGTAGGCCTGATAAGCGTAgc                    <  4:532535/149‑1 (MQ=255)
                                                                                                                               aGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGcc                                                                                        <  1:164604/79‑1 (MQ=255)
                                                                                                                               aGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGcc                                                                                        >  2:164604/1‑79 (MQ=255)
                                                                                                                                gCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGc                                           <  4:369566/123‑1 (MQ=255)
                                                                                                                                gCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGc                                           >  3:369566/1‑123 (MQ=255)
                                                                                                                                gCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGCCATGTAGGCCTGATAAGCGTAGCGCa                 <  1:703919/149‑1 (MQ=255)
                                                                                                                                 cAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGCCATGTAGGCCTGATAAGCGTAGCGCAt                <  4:559318/149‑1 (MQ=255)
                                                                                                                                  aaTAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGCCATGTAGGCCTGATAAGCGTAGCGCATc               <  1:100570/149‑1 (MQ=255)
                                                                                                                                     aaaaaaGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGCCATGTAGGCCTGATAAGCGTAGCGCATCAgg            >  3:432569/1‑149 (MQ=255)
                                                                                                                                     aaaaaaGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACACTGCATGGGCCATGTAGGCCTGATAAGCGTAGCGCATCAgg            >  3:839101/1‑149 (MQ=255)
                                                                                                                                      aaaaaGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGCCATGTAGGCCTGATAAGCGTAGCGCATCAGGc           >  2:720594/1‑149 (MQ=255)
                                                                                                                                        gaaGCCGCACATGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGcc                                                                              <  3:440641/79‑1 (MQ=18)
                                                                                                                                        gaaGCCGCACATGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGcc                                                                              >  4:440641/2‑80 (MQ=17)
                                                                                                                                        gaaGCCGCACATGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGCCATGTAGGCCTGATAAGCGTAg                     >  2:309088/2‑137 (MQ=255)
                                                                                                                                        gaaGCCGCACATGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGCCATGTAGGCCTGATAAGCGTAg                     <  1:309088/136‑1 (MQ=255)
                                                                                                                                         aaGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGCCATGTAGGCCTGATAAGCGTAGCGCATCAGGCAAt        <  4:289197/149‑1 (MQ=255)
                                                                                                                                              gCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCt                                                                                       >  2:219023/1‑65 (MQ=255)
                                                                                                                                              gCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCt                                                                                       <  1:219023/65‑1 (MQ=255)
                                                                                                                                               cATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGCCATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTAcc  <  1:757963/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
TCCAGCTGTAACGCCCAAACTATGGCGAAAGATATCCGCGAACTGCCTGGGTTTCGTATTGAACGGGTGCAGCTTTTCGATATGTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGCCATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTACC  >  NC_000913/899511‑899802

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: