Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 958,773 C→G 7.7% intergenic (+32/‑39) serC → / → aroA 3‑phosphoserine/phosphohydroxythreonine aminotransferase/5‑enolpyruvylshikimate‑3‑phosphate synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913958,7730CG 7.7% 529.0 / 26.8 153intergenic (+32/‑39)serC/aroA3‑phosphoserine/phosphohydroxythreonine aminotransferase/5‑enolpyruvylshikimate‑3‑phosphate synthetase
Reads supporting (aligned to +/- strand):  ref base C (60/81);  new base G (5/7);  total (65/88)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.32e-01

Only 100 of 157 total aligned reads displayed.

GAAAGGTCACCGTGTGGTCGGCGGAATGCGCGCTTCTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCGTTTCTAACCGCGCTTTATTGCTGGCGGCATTAGCACACG  >  NC_000913/958627‑958920
                                                                                                                                                  |                                                                                                                                                   
  aaGGTCACCGTGTGGTCGGCGGAATGCGCGCTTCTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCtgt                                                                                                                                                 <  3:13002/149‑1 (MQ=255)
     gTCACCGTGTGGTCGGCGGAATGCGCGCTTCTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTggg                                                                                                                                              >  1:186829/1‑149 (MQ=255)
      tCACCGTGTGGTCGGCGGAATGCGCGCTTCTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGGCTGGCTGTgggg                                                                                                                                             <  2:64560/149‑1 (MQ=255)
       cACCGTGTGGTCGGCGGAATGCGCGCTTCTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGGCTGGCTGTgggga                                                                                                                                            <  3:562522/149‑2 (MQ=255)
         ccGTGTGGTCGGCGGAATGCGCGCTTCTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGGCTGGCTGTGGGGatt                                                                                                                                          >  3:445032/1‑149 (MQ=255)
           gtgtGGTCGGCGGAATGCGCGCTTCTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGGCTGGCTGTGGGGattaa                                                                                                                                        <  4:445032/149‑3 (MQ=255)
           gtgtGGTCGGCGGAATGCGCGCTTCTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGGCTGGCTGTGGGGattaa                                                                                                                                        <  1:905536/149‑3 (MQ=255)
            tgtgGTCGGCGGAATGCGCGCTTCTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTa                                                                                                                                       <  4:35633/149‑1 (MQ=255)
            tgtgGTCAGCGGAATGCGCGCTTCTATTTATAACGCCATGCCGCTGGACGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTCCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTa                                                                                                                                       <  4:1450/149‑1 (MQ=255)
             gtgGTCGGCGGAATGCGCGCTTCTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTAt                                                                                                                                      <  2:252425/149‑1 (MQ=255)
               ggTCGGCGGAATGCGCGCTTCTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTAttt                                                                                                                                    <  1:25107/149‑1 (MQ=255)
               ggTCGGCGGAATGCGCGCTTCTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTAttt                                                                                                                                    <  2:546155/149‑1 (MQ=255)
                  cggcggAATGCGCGCTTCTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCtg                                                                                                                                 <  2:665374/149‑1 (MQ=255)
                    gcggAATGCGCGCTTCTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCtgtt                                                                                                                               <  4:500634/149‑1 (MQ=255)
                       gAATGCGCGCTTCTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTa                                                                                                                            <  4:321539/149‑1 (MQ=255)
                            cgcgcTTCTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTagagag                                                                                                                       <  2:377061/149‑1 (MQ=255)
                             gcgcTTCTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGGCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAgagt                                                                                                                      <  4:344791/149‑1 (MQ=255)
                             gcgcTTCTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAgagt                                                                                                                      >  3:144733/1‑149 (MQ=255)
                             gcgcTTCTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAgagt                                                                                                                      <  4:861445/149‑1 (MQ=255)
                              cgcTTCTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAgagtt                                                                                                                     <  2:929095/149‑1 (MQ=255)
                                cTTCTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAgagttga                                                                                                                   >  1:364478/1‑149 (MQ=255)
                                cTTCTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAgagttga                                                                                                                   >  1:892028/1‑149 (MQ=255)
                                 ttCTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAgagttgag                                                                                                                  <  3:265075/149‑1 (MQ=255)
                                    tATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTc                                                                                                               >  4:269355/1‑149 (MQ=255)
                                     aTTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCa                                                                                                              <  1:800639/149‑1 (MQ=255)
                                      tttATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCAt                                                                                                             <  1:934378/149‑1 (MQ=255)
                                      tttATAACGCCATGCCGCTCGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTAGTTCATg                                                                                                            <  4:651406/149‑1 (MQ=255)
                                       ttATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATg                                                                                                            <  3:831059/149‑1 (MQ=255)
                                         ataACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCt                                                                                                                                  >  3:181097/1‑125 (MQ=255)
                                         ataACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCt                                                                                                                                  <  4:181097/125‑1 (MQ=255)
                                         ataACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCAAGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGa                                                                                                          >  2:291565/1‑149 (MQ=255)
                                           aaCGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAAt                                                                                                        >  4:224844/1‑149 (MQ=255)
                                               ccATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCt                                                                                                    <  2:155412/149‑1 (MQ=255)
                                               ccATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCt                                                                                                    <  3:762719/149‑1 (MQ=255)
                                               ccATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCt                                                                                                    >  2:817942/1‑149 (MQ=255)
                                                 aTGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCGCAGCCAGCCTGTGGGGTTTTTATCTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGa                                                                                                  <  4:257952/149‑1 (MQ=255)
                                                 aTGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGa                                                                                                  <  4:567530/149‑1 (MQ=255)
                                                   gCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGGCTGGCTGTggggattaa                                                                                                                                        <  4:310754/109‑6 (MQ=255)
                                                   gCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGGCTGGCTGTGGGGattaa                                                                                                                                        >  3:310754/1‑107 (MQ=255)
                                                              gCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCAt                                                                                     <  2:458706/149‑1 (MQ=255)
                                                              gCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCAt                                                                                     <  1:817942/149‑1 (MQ=255)
                                                                gTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTagaga                                                                                                                        >  3:458326/1‑112 (MQ=255)
                                                                gTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTagaga                                                                                                                        <  4:458326/112‑1 (MQ=255)
                                                                   aaaGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTc                                                                                <  4:241905/149‑1 (MQ=255)
                                                                   aaaGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTc                                                                                <  4:832645/149‑1 (MQ=255)
                                                                   aaaGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTc                                                                                >  2:931676/1‑149 (MQ=255)
                                                                    aaGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCg                                                                               >  2:598981/1‑149 (MQ=255)
                                                                     aGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCgt                                                                              >  3:294127/1‑149 (MQ=255)
                                                                     aGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCgt                                                                              <  1:626316/149‑1 (MQ=255)
                                                                          tGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGa                                                                         >  3:793441/1‑149 (MQ=255)
                                                                           gACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGAt                                                                        >  1:689835/1‑149 (MQ=255)
                                                                             cAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAAACCCTGACGTTACAACCCATCGCTCGTGTCGATgg                                                                      <  2:436653/149‑1 (MQ=255)
                                                                              aGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCAt                                                                                                             >  3:723866/1‑109 (MQ=255)
                                                                              aGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCAt                                                                                                             <  4:723866/109‑1 (MQ=255)
                                                                              aGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGc                                                                     >  4:372066/1‑149 (MQ=255)
                                                                              aGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGc                                                                     >  4:893164/1‑149 (MQ=255)
                                                                               gACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCa                                                                    <  3:269355/149‑1 (MQ=255)
                                                                               gACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCa                                                                    <  3:893164/149‑1 (MQ=255)
                                                                                aCTTCATGGTTCAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCAGAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCAGGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCAc                                                                   >  1:762890/1‑149 (MQ=255)
                                                                                  ttCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAAt                                                                                                        <  2:380419/110‑1 (MQ=255)
                                                                                  ttCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTa                                                                 >  1:492164/1‑149 (MQ=255)
                                                                                  ttCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTa                                                                 >  3:879189/1‑149 (MQ=255)
                                                                                     aTGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGCTACAACCCATCGCTCGTGTCGATGGCACTATTa                                                              <  2:364478/149‑1 (MQ=255)
                                                                                      tGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTaa                                                             <  2:186829/149‑1 (MQ=255)
                                                                                                cGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGt                                                   <  2:757671/149‑1 (MQ=255)
                                                                                                  aaCGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTc                                                 >  2:629971/1‑149 (MQ=255)
                                                                                                   aCGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTcc                                                <  3:184208/149‑1 (MQ=255)
                                                                                                   aCGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTcc                                                <  4:144733/149‑1 (MQ=255)
                                                                                                      ccGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAag                                             >  4:877159/1‑149 (MQ=255)
                                                                                                       cGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAaga                                            >  1:822660/1‑149 (MQ=255)
                                                                                                        gTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAagag                                           >  4:286509/1‑149 (MQ=255)
                                                                                                         tCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAAGAGc                                          >  2:626190/1‑149 (MQ=255)
                                                                                                           aCGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCGt                                        <  4:261704/149‑1 (MQ=255)
                                                                                                           aCGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCGt                                        <  2:705577/149‑1 (MQ=255)
                                                                                                            aggTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCGtt                                       <  1:291565/148‑1 (MQ=255)
                                                                                                             ggTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCGttt                                      <  1:408706/149‑1 (MQ=255)
                                                                                                             ggTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCGttt                                      <  2:30665/149‑1 (MQ=255)
                                                                                                                 aaTGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCGTTTCTaa                                  <  1:628846/149‑1 (MQ=255)
                                                                                                                    gCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCaa                                              <  1:212664/134‑1 (MQ=255)
                                                                                                                      cGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCGTTTCTAACcgcg                             >  2:77855/1‑149 (MQ=255)
                                                                                                                       gAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATc                                                                                                       >  3:599851/1‑74 (MQ=255)
                                                                                                                           ttttGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATcc                                                                                                      >  1:683346/1‑71 (MQ=255)
                                                                                                                            tttGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCGTTTCTAACCGCGCTTTAt                       <  4:793441/149‑1 (MQ=255)
                                                                                                                             ttGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCGTTTCTAACCGCGCTTTAtt                      <  3:877159/149‑1 (MQ=255)
                                                                                                                             ttGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCGTTTCTAACCGCGCTTTAtt                      <  1:798733/149‑1 (MQ=255)
                                                                                                                              tGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCGTTTCTAACCGCGCTTTATTg                     <  1:561257/149‑1 (MQ=255)
                                                                                                                               gCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCGTTTCTAACCGCGCTTTATTGc                    <  1:598981/149‑1 (MQ=255)
                                                                                                                                 ttAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCGCGGTTCCAAGAGCGTTTCTAACCGCGCTTTATTGCTg                  >  3:601198/1‑149 (MQ=255)
                                                                                                                                   aaTCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCGTTTCTAACCGCGCTTTATTGCTggc                >  4:385311/1‑149 (MQ=255)
                                                                                                                                    aTCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCGTTTCTAACCGCGCTTTATTGCTggcg               <  3:358958/149‑1 (MQ=255)
                                                                                                                                     tCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCGTTTCTaa                                  >  2:804457/1‑129 (MQ=255)
                                                                                                                                       cccACAGGCTGGCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCGTTTCTAACCGCGCTTTATTGCTGGCGGCa            >  4:364740/1‑149 (MQ=255)
                                                                                                                                        ccACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCaa                                              <  1:307693/114‑1 (MQ=255)
                                                                                                                                        ccACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCGTTTCTAACCGCGCTTTATTGCTGGCGGCAt           <  3:635164/149‑1 (MQ=255)
                                                                                                                                        ccACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCGTTTCTAACCGCGCTTTATTGCTGGCGGCAt           <  3:228589/149‑1 (MQ=255)
                                                                                                                                         cacaGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCGttt                                      >  3:429102/1‑121 (MQ=255)
                                                                                                                                          acaGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCGTTTCTAACCGCGCTTTATTGCTGGCGGCATTa         <  4:615486/149‑1 (MQ=255)
                                                                                                                                           ctgccagccTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCGTTTCTAACCGCGCTTTATTGCTGGCGGCATTAg        <  2:271079/147‑1 (MQ=255)
                                                                                                                                           cagccagcCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCGTTTCTAACCGCGCTTTATTGCTGGCGGCATTAg        <  2:607380/149‑1 (MQ=255)
                                                                                                                                                 gccTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCGTTTCTAACCGCGCTTTATTGCTGGCGGCATTAGCACACg  <  1:544779/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
GAAAGGTCACCGTGTGGTCGGCGGAATGCGCGCTTCTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCGTTTCTAACCGCGCTTTATTGCTGGCGGCATTAGCACACG  >  NC_000913/958627‑958920

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: