Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,174,080 T→C 11.6% intergenic (‑116/+12) mfd ← / ← ycfT transcription‑repair coupling factor/inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,174,0800TC11.6% 366.6 / 30.1 130intergenic (‑116/+12)mfd/ycfTtranscription‑repair coupling factor/inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (60/54);  new base C (9/6);  total (70/60)
Fisher's exact test for biased strand distribution p-value = 7.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.50e-01

Only 100 of 130 total aligned reads displayed.

GGGCAGCGTATAACGATATTGTTCAGGCATAACGGTTGTCAGATTCTCGTTAGGATATGCCTCAACATATGGGGGCATATCTCTGATAAGCAATGCTGTTCATTATCCGTAATCGACATGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGAGAACAACAGATCGCTGAATGCACGCTGTGAAAGTTTTCTTACCAGCAAGCCCGCAACAGTACAGATGAACAAACTGACAAAGGGATAAAC  >  NC_000913/1173935‑1174223
                                                                                                                                                 |                                                                                                                                               
gggCAGCGTATAACGATATTGTTCAGGCATAACGGTTGTCAGATTCTCGTTAGGATATGCCTCAACATATGGGGGCATATCTCTGATAAGCAATGCTGTTCATTATCCGTAATCGACATGGTTTAGCAAATTGAATCGCCCCGGCTggg                                                                                                                                              >  4:310853/1‑149 (MQ=255)
     gCGTATAACGATCTTGTTCAGGCATAACGGTTGTCAGATTCTCGTTAGGATATGCCTCAACATATGGGGGCATATCTCTGATAAGCAATGCTGTTCATTATCCGTAATCGACATGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGAt                                                                                                                                         <  1:740015/149‑1 (MQ=255)
     gCGTATAACGATATTGTTCAGGCATAACGGTTGTCAGATTCTCGTTAGGATATGCCTCAACATATGGGGGCATATCTCTGATAAGCAATGCTGTTCATTATCCGTAATCGACATGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGAt                                                                                                                                         <  3:242859/149‑1 (MQ=255)
     gCGTATAACGATATTGTTCAGGCATAACGGTTGTCAGATTCTCGTTAGGATATGCCTCAACATATGGGGGCATATCTCTGATAAGCAATGCTGTTCATTATCCGTAATCGACATGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGAt                                                                                                                                         >  3:802021/1‑149 (MQ=255)
     gCGTATAACGATATTGTTCAGGCATAACGGTTGTCAGATTCTCGTTAGGATATGCCTCAACATATGGGGGCATATCTCTGATAAGCAATGCTGTTCATTATCCGTAATCGACATGGTTTAGCAAATTGAATCGCCCAAGCCGGGGCGAt                                                                                                                                         <  3:167287/149‑1 (MQ=255)
       gTATAACGATATTGTTCAGGCATAACGGTTGTCAGATTCTCGTTAGGATATGCCTCAACATATGGGGGCATATCTCTGATAAGCAATGCTGTTCATTATCCGTAATCGACATGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATgg                                                                                                                                       <  3:809165/149‑1 (MQ=255)
       gTATAACGATATTGTTCAGCCATAACGGTTGTCAGATTCTCGTTAGGATATGCCTCAACATATGGGGGCATATCTCTGATAAGCAATGCTGTTCATTATCCGTAATCGACATGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATgg                                                                                                                                       <  4:630104/149‑1 (MQ=255)
          taACGATATTGTTCAGGCATAACGGTTGTCAGATTCTCGTTAGGATATGCCTCAACATATGGGGGCATATCTCTGATAAGCAATGCTGTTCATTATCCGTAATCGACATGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATgg                                                                                                                                       >  2:393503/1‑146 (MQ=255)
          taACGATATTGTTCAGGCATAACGGTTGTCAGATTCTCGTTAGGATATGCCTCAACATATGGGGGCATATCTCTGATAAGCAATGCTGTTCATTATCCGTAATCGACATGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATgg                                                                                                                                       <  1:393503/146‑1 (MQ=255)
               atatTGTTCAGGCATAACGGTTGTCAGATTCTCGTTAGGATATGCCTCAAGATATGGGGGCATATCTCTGATAAGCAATGCTGTTCATTATCCGTAATCGAGATGGTTTAGCAAATTGAATCGCCCCAGCCGGGGCGAttcaattt                                                                                                                                  >  4:621282/1‑139 (MQ=255)
               atatTGTTCAGGCATAACGGTTGTCAGATTCTCGTTAGGATATGCCTCAACATATGGGGGCATATCTCTGATAAGCAATGCTGTTCATTATCCGTAATCGACATGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATGGCTTAGCgg                                                                                                                               <  3:171971/149‑1 (MQ=255)
               atatTGTTCAGGCATAACGGTTGTCAGATTCTCGTTAGGATATGCCTCAACATATGGGGGCATATCTCTGATAAGCAATGCTGTTCATTATCCGTAATCGACATGGTTTAGCAAATTGAATCGCCCCAGCCGGGGCGAttcaattt                                                                                                                                  <  3:621282/146‑8 (MQ=255)
                tatTGTTCAGGCATAACGGTTGTCAGATTCTCGTTAGGATATGCCTCAACATATGGGGGCATATCTCTGATAAGCAATGCTGTTCATTATCCGTAATCGACATGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATGGCTTAGCggg                                                                                                                              >  3:555204/1‑149 (MQ=255)
                     ttCAGGCATAACGGTTGTCAGATTCTCGTTAGGATATGCCTCAACATATGGGGGCATATCTCTGATAAGCAATGCTGTTCATTATCCGTAATCGACATGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTaa                                                                                                                         >  1:770956/1‑149 (MQ=255)
                         ggCATAACGGTTGTCAGATTCTCGTTAGGATATGCCTCAACATATGGGGGCATATCTCTGATAAGCAATGCTGTTCATTATCCGTAATCGACATGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGa                                                                                                                     <  1:333789/149‑1 (MQ=255)
                            aTAACGGTTGTCAGATTCTCGTTAGGATATGCCTCAACATATGGGGGCATATCTCTGATAAGCAATGCTGTTCATTATCCGTAATCGACATGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACgg                                                                                                                  >  4:286534/1‑149 (MQ=255)
                            aTAACGGTTGTCAGATTCTCGTTAGGATATGCCTCAACATATGGGGGCATATCTCTGATAAGCAATGCTGTTCATTATCCGTAATCGACATGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACgg                                                                                                                  >  2:504866/1‑149 (MQ=255)
                                cGGTTGTCAGATTCTCGTTAGGATATGCCTCAACATATGGGGGCATATCTCTGATAAGCAATGCTGTTCATTATCCGTAATCGACATGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGc                                                                                                              <  2:796377/149‑1 (MQ=255)
                                   ttGTCAGATTCTCGTTAGGATATGCCTCAACATATGGGGGCATATCTCTGATAAGCAATGCTGTTCATTATCCGTAATCGACATGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCggg                                                                                                           <  4:555204/149‑1 (MQ=255)
                                     gTCAGATTCTCGTTAGGATATGCCTCAACATATGGGGGCATATCTCTGATAAGCAATGCTGTTCATTATCCGTAATCGACATGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCa                                                                                                         >  4:316646/1‑149 (MQ=255)
                                      tCAGATTCTCGTTAGGATATGTCTCAACATATGGGGTCATATCTCTGATAAGCAATGCTGTTCATTATCCGTAATCGACATGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATTGCTTAGCGGGAGTAACTGACGGCAGCGGGCAg                                                                                                        <  3:310853/149‑1 (MQ=255)
                                      tCAGATTCTCGTTAGGATATGCCTCAACATATGGGGGCATATCTCTGATAAGCAATGCTGTTCATTATCCGTAATCGACATGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTaa                                                                                                                         <  1:483122/132‑1 (MQ=255)
                                       cAGATTCTCGTTAGGATATGCCTCAACATATGGGGGCATATCTCTGATAAGCAATGCTGTTCATTATCCGTAATCGACATGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGt                                                                                                       <  4:694244/149‑1 (MQ=255)
                                        aGATTCTCGTTAGGATATGCCTCAACATATGGGGGCATATCTCTGATAAGCAATGCTGTTCATTATCCGTAATCGACATGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTg                                                                                                      >  2:749518/1‑149 (MQ=255)
                                              tcGTTAGGATATGCCTCAACATATGGGGGCATATCTCTGATAAGCAATGCTGTTCATTATCCGTAATCGACATGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCg                                                                                                >  1:21759/1‑149 (MQ=255)
                                               cGTTAGGATATGCCTCAACATATGGGGGCATATCTCTGATAAGCAATGCTGTTCATTATCCGTAATCGACATGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCgg                                                                                               <  2:557841/149‑1 (MQ=255)
                                                gTTAGGATATGCCTCAACATATGGGGGCATATCTCTGATAAGCAATGCTGTTCATTATCCGTAATCGACATGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCggg                                                                                              <  1:125152/149‑1 (MQ=255)
                                                gTTAGGATATGCCTCAACATATGGGGGCATATCTCTGATAAGCAATGCTGTTCATTATCCGTAATCGACATGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCggg                                                                                              <  1:778046/149‑1 (MQ=255)
                                                gTTAGGATATGCCTCAACATATGGGGGCATATCTCTGATAAGCAATGCTGTTCATTATCCGTAATCGACATGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCggg                                                                                              >  1:642478/1‑149 (MQ=255)
                                                        atGCCTCAACATATGGGGGCATATCTCTGATAAGCAATGCTGTTCATTATCCGTAATCGACATGGTTTAGCAAATTGAATCGCCCCGGCCGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGAGaacaa                                                                                      >  3:172605/1‑149 (MQ=255)
                                                           ccTCAACATATGGGGGCATATCTCTGATAAGCAATGCTGTTCATTATCCGTAATCGACATGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGAGAACAACAg                                                                                   >  3:105149/1‑149 (MQ=255)
                                                            cTCAACATATGGGGGCATATCTCTGATAAGCAATGCTGTTCATTATCCGTAATCGACATGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGAGAACAACAGa                                                                                  >  1:745062/1‑149 (MQ=255)
                                                            cTCAACATATGGGGGCATATCTCTGATAAGCAATGCTGTTCATTATCCGTAATCGACATGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGAGAACAACAGa                                                                                  >  2:87085/1‑149 (MQ=255)
                                                             tCAACATATGGGGGCATATCTCTGATAAGCAATGCTGTTCATTATCCGTAATCGACATGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGAGAACAACAGAt                                                                                 <  3:202882/149‑1 (MQ=255)
                                                               aaCATATGGGGGCATATCTCTGATAAGCAATGCTGTTCATTATCCGTAATCGACATGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGAGAACAACAGATCg                                                                               >  1:545415/1‑149 (MQ=255)
                                                                 cATATGGGGGCATATCTCTGATCAGCAATTCTGTTCATTATCCGTAATCGACATGGTTTAGCAAATTGAATCGACCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGAGAACAACAGATCGCt                                                                             <  1:674215/149‑1 (MQ=255)
                                                                           cATATCTCTGATAAGCAATGCTGTTCATTATCCGTAATCGACATGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGAGAACAACAGATCGCTGAATGCACGc                                                                   >  3:36749/1‑149 (MQ=255)
                                                                             tatCTCTGATAAGCAATGCTGTTCATTATCCGTAATCGACATGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGga                                                                                            >  1:650768/1‑122 (MQ=255)
                                                                             tatCTCTGATAAGCAATGCTGTTCATTATCCGTAATCGACATGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGga                                                                                            <  2:650768/122‑1 (MQ=255)
                                                                              atCTCTGATAAGCAATGCTGTTCATTATCCGTAATCGACATGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGAGAACAACAGATCGCTGAATGCACGCtgt                                                                >  4:141862/1‑149 (MQ=255)
                                                                               tctcTGATAAGCAATGCTGTTCATTATCCGTAATCGACATGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGAGaa                                                                                         <  2:231161/123‑1 (MQ=255)
                                                                               tctcTGATAAGCAATGCTGTTCATTATCCGTAATCGACATGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGAGaa                                                                                         >  1:231161/1‑123 (MQ=255)
                                                                               tctcTGATAAGCAATGCTGTTCATTATCCGTAATCGACATGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGAGGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGAGAACAACAGATCGCTGAATGCACGCtgtg                                                               >  3:822883/1‑149 (MQ=255)
                                                                                ctctGATAAGCAATGCTGTTCATTATCCGTAATCGACATGGTTTAGCAAATTGAATCGCCCCGGCTGGGGTGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGAGAACAACAGATCGCTGAATGCACGCTGTGa                                                              >  3:458542/1‑149 (MQ=255)
                                                                                 tctGATAAGCAATGCTGTTCATTATCCGTAATCGACATGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGAGAACAACAGATCGCTGAATGCACGCTGTGaa                                                             >  3:2957/1‑149 (MQ=255)
                                                                                 tctGATAAGCAATGCTGTTCATTATCCGTAATCGACATGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGAGAACAACAGATCGCTGAATGCACGCTGTGaa                                                             <  3:450982/149‑1 (MQ=255)
                                                                                   tGATAAGCAATGCTGTTCATTATCCGTAATCGACATGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCATTGAAGGCGGGGAGAACAACAGATCGCTGAATGCACGCTGTGAAAg                                                           >  4:311684/1‑149 (MQ=255)
                                                                                   tGATAAGCAATGCTGTTCATTATCCGTAATCGACATGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGAGAACAACAGATCGCTGAATGCACGCTGTGAAAg                                                           >  3:687120/1‑149 (MQ=255)
                                                                                   tGATAAGCAATGCTGTTCATTATCCGTAATCGACATGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGAGAACAACAGATCGCTGAATGCACGCTGTGAAAg                                                           >  2:582473/1‑149 (MQ=255)
                                                                                    gATAAGCAATGCTGTTCATTATCCGTAATCGACATGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGAGAACAACAGATCGCTGAATGCACGCTGTGAAAGt                                                          <  1:749518/149‑1 (MQ=255)
                                                                                      tAAGCAATGCTGTTCATTATCCGTAATCGACATGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGAGAACAACAGATCGCTGAATGCACGCTGTGAAAGttt                                                        <  4:415695/149‑1 (MQ=255)
                                                                                      tAAGCAATGCTGTTCATTATCCGTAATCGACATGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGAGAACAACAGATCGCTGAATGCACGCTGTGAAAGttt                                                        >  3:88268/1‑149 (MQ=255)
                                                                                       aaGCAATGCTGTTCATTATCCGTAATCGACATGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGAGAACAACAGATCGCTGAATGCACGCTGTGAAAGtttt                                                       <  1:582473/149‑1 (MQ=255)
                                                                                          cAATGCTGTTCATTATCCGTAATCGACATGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGAGAACAACAGATCGCTGAATGCACGCTGTGAAAGTTTTCtt                                                    >  3:546022/1‑149 (MQ=255)
                                                                                           aaTGCTGTTCATTATCCGTACTCGAGATGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCgg                                                                                               <  3:670725/105‑1 (MQ=255)
                                                                                           aaTGCTGTTCATTATCCGTAATCGACATGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCgg                                                                                               >  4:670725/1‑105 (MQ=255)
                                                                                            aTGCTGTTCATTATCCGTAATCGACATGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGAGAACAACAGATCGCTGAATGCACGCTGTGAAAGTTTTCTTAc                                                  <  1:801879/149‑1 (MQ=255)
                                                                                                 gTTCATTATCCGTAATCGACATGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGAGAACAACAGATCGCTGAATGCACGCTGTGaaa                                                            <  4:233992/134‑1 (MQ=255)
                                                                                                 gTTCATTATCCGTAATCGACATGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGAGAACAACAGATCGCTGAATGCACGCTGTGaaa                                                            >  3:233992/1‑134 (MQ=255)
                                                                                                  ttCATTATCCGTAATCGACATGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGAGAACAACAGATCGCTGAATGCACGCTGTGAAAGTTTTCTTACCAGCaa                                            <  4:450603/149‑1 (MQ=255)
                                                                                                     aTTATCCGTAATCGACATGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGAGAACAACAGATCGCTGAATGCACGCTGTGAAAGTTTTCTTACCAGCAAGcc                                         >  4:202963/1‑149 (MQ=255)
                                                                                                      ttATCCGTAATCGACATGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGAGAACAACAGATCGCTGAATGCACGCTGTGAAAGTTTTCTTACCAGCAAGccc                                        >  3:498277/1‑149 (MQ=255)
                                                                                                      ttATCCGTAATCGACATGGTTTAGCAAATCGAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGAGAACAACAGATCGCTGAATGCACGCTGTGAAAGTTTTCTTACCAGCAAGccc                                        >  3:752050/1‑149 (MQ=255)
                                                                                                           cGTACTCGACATGGTTTAGCAAATAGAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGAGAACAACAGATCGCTGAATGCACGCTGTGAAAGTTTTCTTACCAGCAAGCCCGCAAc                                   >  1:795166/1‑149 (MQ=255)
                                                                                                            gTAATCGACATGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGAGAACAACAGATCGCTGAATGCa                                                                      <  3:915286/113‑1 (MQ=255)
                                                                                                            gTAATCGACATGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGAGAACAACAGATCGCTGAATGCa                                                                      >  4:915286/1‑113 (MQ=255)
                                                                                                            gTAATCGACATGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGAGAACAACAGATCGCTGAATGCACGCTGTGAAAGTTTTCTTACCAGCAAGCCCGCAACa                                  >  1:273519/1‑149 (MQ=255)
                                                                                                                 cGACATGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGAGAACAACAGATCGCTGAATGCACGCTGTGAAAGTTTTCTTACCAGCAAGCCCGCAACAGTACa                             <  3:141862/149‑1 (MQ=255)
                                                                                                                   aCATGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGAGAACAACAGATCGCTGAATGCACGCTGTGAAAGTTTTCTTACCAGCAAGGCCGCAACAGTACAGa                           <  2:21759/149‑1 (MQ=255)
                                                                                                                   aCATGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGAGAACAACAGATCGCTGAATGCACGCTGTGAAAGTTTTCTTACCAGCAAGCCCGCAACAGTACAGa                           <  4:88268/149‑1 (MQ=255)
                                                                                                                    cATGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGAGAACAACAGATc                                                                                <  4:137256/95‑1 (MQ=255)
                                                                                                                    cATGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGAGAACAACAGATc                                                                                >  3:137256/1‑95 (MQ=255)
                                                                                                                     aTGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGAGAACAACAGATCGCTGAATGCACGCTGTGAAAGTTTTCTTACCAGCAAGCCCGCAACAGTACAGATg                         <  3:589205/149‑1 (MQ=255)
                                                                                                                     aTGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGAGAACAACAGATCGCTGAATGCACGCTGTGAAAGTTTTCTTACCAGCAAGCCCGCAACAGTACAGATg                         >  4:276784/1‑149 (MQ=255)
                                                                                                                     aTGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGAGAACAACAGATCGCTGAATGCACGCTGTGAAAGTTTTCTTACCAGCAAGCCCGCAACAGTACAGATg                         >  2:112332/1‑149 (MQ=255)
                                                                                                                      tGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGAGAACAACAGATCGCTGAATGCACGCTGTGAAAGTTTTCTTACCAGCAAGCCCGCAACAGTACAGATGa                        >  3:57351/1‑149 (MQ=255)
                                                                                                                      tGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGAGAACAACAGATCGCTGAATGCACGCTGTGAAAGTTTTCTTACCAGCAAGCCCGCAACAGTACAGATGa                        <  2:545415/149‑1 (MQ=255)
                                                                                                                       ggTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGAGAACAACAGATCGCTGAATGCACGCTGTGAAAGTTTTCTTACCAGCAAGCCCGCAACAGTACAGATGaa                       <  1:87085/149‑1 (MQ=255)
                                                                                                                       ggTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGAGAACAACAGATCGCTGAATGCACGCTGTGAAAGTTTTCTTACCAGCAAGCCCGCAACAGTACAGATGaa                       <  4:36749/149‑1 (MQ=255)
                                                                                                                             gCAAATTGAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGAGAACAACAGATCGCTGAATGCACGCTGTGAAAGTTTTCTTACCAGCAAGCCCGCAACAGTACAGATGAACAAACt                 >  3:651344/1‑149 (MQ=255)
                                                                                                                                aaTTGAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGAGAACAACAGATCGCTGAATGCACGCTGTGAAAGTTTTCTTACCAGCAAGCCCGCAACAGTACAGATGAACAAACTGAc              >  1:682562/1‑149 (MQ=255)
                                                                                                                                   tgtaTCGCCCCGGCTGGGGCGGTGGCTTAGCGGGAGTAGCTGACGGCAGCGGGCAGTGAAGGCGGGGAGAACAACAGTTCGCTGAGTGCACGCTGTGAAAGTTTTCTTACCATCAAGCCCGCAACAGTACAGATGAACAAACTGACaaa           <  4:822883/146‑1 (MQ=255)
                                                                                                                                   tGAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGAGAACAACAGATCGCTGAATGCACGCTGTGAAAGTTTTCTTACCAGCAAGCCCGCAACAGTACAGATGAACAAACTGACaaa           >  1:890046/1‑149 (MQ=255)
                                                                                                                                    gAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGAGAACAACAGATCGCTGAATGCACGCTGTGAAAGTTTTCTTACCAGCAAGCCCGCAACAGTACAGATGAACAAACTGACAAAg          <  3:202963/149‑1 (MQ=255)
                                                                                                                                     caTCGCCCCAGCCGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGAGAACAACAGATCGCTGAATGCACGCTGTGAAAGTTTTCTTACCa                                                <  3:141594/109‑1 (MQ=255)
                                                                                                                                      aTCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGAGAACAACAGATCGCTGAATGCACGCTGTGAAAGTTTTCTTACCAGCAAGCCCGCAACAGTACAGAt                          >  3:27244/1‑131 (MQ=255)
                                                                                                                                      aTCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGAGAACAACAGATCGCTGAATGCACGCTGTGAAAGTTTTCTTACCAGCAAGCCCGCAACAGTACAGATGAACAAACTGACAAAggg        >  2:280495/1‑149 (MQ=255)
                                                                                                                                      aTCGCCCCAGCCGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGAGaacaa                                                                                      <  4:656666/71‑1 (MQ=255)
                                                                                                                                      aTCGCCCCAGCCGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGAGAACAACAGATCGCTGAATGCACGCTGTGAAAGtttt                                                       <  3:638488/102‑1 (MQ=255)
                                                                                                                                      aTCGCCCCAGCCGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGAGAACAACAGATCGCTGAATGCACGCTGTGAAAGtttt                                                       >  4:638488/1‑102 (MQ=255)
                                                                                                                                      aTCGCCCCAGCCGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGAGAACAACAGATCGCTGAATGCACGCTGTGAAAGTTTTCTTACCAGCAAGCCCGCAACAGTACAGATGa                        <  1:35726/133‑1 (MQ=255)
                                                                                                                                      aTCGCCCCAGCCGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGAGAACAACAGATCGCTGAATGCACGCTGTGAAAGTTTTCTTACCAGCAAGCCCGCAACAGTACAGATGa                        >  2:35726/1‑133 (MQ=255)
                                                                                                                                      aTCGCCCCAGCCGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGAGAACAACAGATCGCTGAATGCACGCTGTGAAAGTTTTCTTACCAGCAAGCCCGCAACAGTACAGATGAACAAACTGACAAAggg        >  2:901256/1‑149 (MQ=255)
                                                                                                                                      aTCGCCCCAGCCGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGAGAACAACAGATCGCTGAATGCACGCTGTGAAAGTTTTCTTACCAGCAAGCCCGCAACAGTACAGATGAACAAACTGACAAAggg        >  2:442788/1‑149 (MQ=255)
                                                                                                                                      aTCGCCCCAGCCGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGAGAACAACAGATCGCTGAATGCACGCTGTGAAAGTTTTCTTACCAGCAAGCCCGCAACAGTACAGATGAACAAACTGACAAAggg        >  3:3377/1‑149 (MQ=255)
                                                                                                                                          ccccGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGAGAACAACAGATCGCTGAATGCACGCTGTGAAAGTTTTTTTACCAGCAAGCCCGCAACAGTACAGATGAACAAACTGACAAAGGGATaa    >  2:565553/1‑149 (MQ=255)
                                                                                                                                          ccccGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGAGAACAACAGATCGCTGAATGCACGCTGTGAAAGTTTTCTTACCAGCAAGCCCGCAACAGTACAGAt                          <  4:346842/127‑1 (MQ=255)
                                                                                                                                          ccccGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGAGAACAACAGATCGCTGAATGCACGCTGTGAAAGTTTTCTTACCAGCAAGCCCGCAACAGTACAGAt                          >  3:346842/1‑127 (MQ=255)
                                                                                                                                            ccGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGAGAACAACAGATCGCTGAATGCACGCTGTGAAAGTTTTCTTACCAGCAAGCCCGCAACAGTACAGATGAACAAACTGACAAAGGGATaaa   >  3:677117/1‑148 (MQ=255)
                                                                                                                                            ccGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGAGAACAACAGATCGCTGAATGCACGCTGTGAAAGTTTTCTTACCAGCAAGCCCGCAACAGTACAGATGAACAAACTGACAAAGGGATaaa   <  4:677117/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
GGGCAGCGTATAACGATATTGTTCAGGCATAACGGTTGTCAGATTCTCGTTAGGATATGCCTCAACATATGGGGGCATATCTCTGATAAGCAATGCTGTTCATTATCCGTAATCGACATGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGAGAACAACAGATCGCTGAATGCACGCTGTGAAAGTTTTCTTACCAGCAAGCCCGCAACAGTACAGATGAACAAACTGACAAAGGGATAAAC  >  NC_000913/1173935‑1174223

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: