Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,330,399 A→T 6.6% intergenic (+18/+18) sohB → / ← yciN inner membrane protein, S49 peptidase family protein/DUF2498 protein YciN

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,330,3990AT 6.6% 314.5 / 10.2 91intergenic (+18/+18)sohB/yciNinner membrane protein, S49 peptidase family protein/DUF2498 protein YciN
Reads supporting (aligned to +/- strand):  ref base A (44/41);  new base T (2/4);  total (46/45)
Fisher's exact test for biased strand distribution p-value = 4.34e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.38e-01

GTCAATGTACGCTATATGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCGGTAAATACCAG  >  NC_000913/1330262‑1330542
                                                                                                                                          |                                                                                                                                               
gTCAATGTACGCTATATGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGTCTTAGCCTCGCGt                                                                                                                                      <  2:490696/149‑1 (MQ=255)
 tCAATGTACGCTATATGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGtt                                                                                                                                     >  1:783213/1‑149 (MQ=255)
  cAATGTACGCTATATGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGTCTTAGCCTCGCGttt                                                                                                                                    <  3:316804/149‑1 (MQ=255)
  cAATGTACGCTATATGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGTCTTAGCCTCGCGttt                                                                                                                                    <  1:719060/149‑1 (MQ=255)
   aaTGTACGCTATATGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGtttt                                                                                                                                   <  1:454436/149‑1 (MQ=255)
   aaTGTACGCTATATGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGtttt                                                                                                                                   >  1:429752/1‑149 (MQ=255)
     tGTACGCTATATGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTcgc                                                                                                                                        >  3:230745/1‑142 (MQ=255)
     tGTACGCTATATGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTcgc                                                                                                                                        <  4:230745/142‑1 (MQ=255)
        aCGCTATATGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCttt                                                                                                                              <  3:486646/149‑1 (MQ=255)
          gCTATATGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTaa                                                                                                                            <  1:336156/149‑1 (MQ=255)
            tataTGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATc                                                                                                                          <  2:735790/149‑1 (MQ=255)
            tataTGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATc                                                                                                                          <  4:343295/149‑1 (MQ=255)
               atGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAAc                                                                                                                       <  2:639595/149‑1 (MQ=255)
                       tAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAgatgat                                                                                                               >  3:809997/1‑149 (MQ=255)
                       tAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAgatgat                                                                                                               >  2:419925/1‑149 (MQ=255)
                         aaCGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGatat                                                                                                             >  2:104067/1‑149 (MQ=255)
                          aCGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATAtt                                                                                                            >  3:272645/1‑149 (MQ=255)
                             aCTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATAttttt                                                                                                         <  2:783213/149‑1 (MQ=255)
                               tCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCt                                                                                                       >  1:341077/1‑149 (MQ=255)
                               tCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCt                                                                                                       <  2:351463/149‑1 (MQ=255)
                               tCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCt                                                                                                       <  1:895584/149‑1 (MQ=255)
                                 aTTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGa                                                                                                     >  3:404137/1‑149 (MQ=255)
                                  ttGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGaa                                                                                                    >  4:748918/1‑149 (MQ=255)
                                   tGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGaaa                                                                                                   <  1:104067/149‑1 (MQ=255)
                                   tGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGaaa                                                                                                   <  1:271848/149‑1 (MQ=255)
                                      ccGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGca                                                                                                >  1:714263/1‑149 (MQ=255)
                                      ccGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGca                                                                                                >  3:585449/1‑149 (MQ=255)
                                         aTTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACAt                                                                                             >  2:305450/1‑149 (MQ=255)
                                            cACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATggg                                                                                          >  3:846842/1‑149 (MQ=255)
                                             aCCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATGTTTTTCTGAAAGCACATGGGc                                                                                         >  2:641051/1‑149 (MQ=255)
                                              ccGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGcc                                                                                        <  2:341077/149‑1 (MQ=255)
                                               cGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCa                                                                                       >  2:705016/1‑149 (MQ=255)
                                                ggCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAg                                                                                      <  1:207787/149‑1 (MQ=255)
                                                 gCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAgg                                                                                     >  4:212156/1‑149 (MQ=255)
                                                 gCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAgg                                                                                     >  1:127032/1‑149 (MQ=255)
                                                   aGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGgtg                                                                                   >  1:144827/1‑149 (MQ=255)
                                                      gcgGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATCTTTTTCTGAAAGCACATGGGCCAGGCGttt                                                                                <  4:583478/149‑1 (MQ=255)
                                                      gcgGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCATTTTGCTTTAATCAACTAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGttt                                                                                <  4:763714/149‑1 (MQ=255)
                                                         gCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGACGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGaa                                                                             <  4:794380/149‑1 (MQ=255)
                                                          cAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAAc                                                                            <  4:283353/149‑1 (MQ=255)
                                                          cAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAAc                                                                            <  3:88418/149‑1 (MQ=255)
                                                               agCGCCGATCGATTGTTGCTTCGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATAtt                                                                       <  3:559757/149‑1 (MQ=255)
                                                                  gcCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGTCTTAGCCTCGCGtttgtc                                                                                                                                 <  3:55954/88‑4 (MQ=37)
                                                                  gcCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGTCTTAGCCTCGCGtttgtc                                                                                                                                 >  4:55954/1‑85 (MQ=37)
                                                                  gcCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTaaa                                                                    <  4:435619/149‑1 (MQ=255)
                                                                  gcCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTaaa                                                                    >  4:429423/1‑149 (MQ=255)
                                                                   ccGATCGATTGTTGCTACGCTGGTGGTAGCGGGGTCAAAAGCCATTGATGTTAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACCTATTAAac                                                                   >  3:573127/1‑149 (MQ=255)
                                                                    cgatcgatTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAaca                                                                  <  1:419925/149‑1 (MQ=255)
                                                                          atTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCgg                                                            >  2:416133/1‑149 (MQ=255)
                                                                          atTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCgg                                                            >  3:249830/1‑149 (MQ=255)
                                                                           ttgttgCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGt                                                           <  1:431658/149‑1 (MQ=255)
                                                                           ttgttgCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGt                                                           <  2:716410/149‑1 (MQ=255)
                                                                           ttgttgCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGt                                                           >  2:229915/1‑149 (MQ=255)
                                                                           ttgttgCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGt                                                           >  1:822695/1‑149 (MQ=255)
                                                                           tngttgCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGt                                                           >  3:94853/1‑149 (MQ=255)
                                                                                  tACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTATAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAga                                                                                                                   <  4:149578/86‑1 (MQ=255)
                                                                                  tACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAga                                                                                                                   >  3:149578/1‑86 (MQ=255)
                                                                                  tACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTg                                                    <  4:888888/149‑1 (MQ=255)
                                                                                      cTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCttaatatgt                                                <  2:821319/149‑8 (MQ=255)
                                                                                      cTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTg                                                >  4:903367/1‑149 (MQ=255)
                                                                                         gtggCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTg                                             <  3:63323/149‑1 (MQ=255)
                                                                                         gtggCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTg                                             <  4:585449/149‑1 (MQ=255)
                                                                                          tggCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTgg                                            <  4:249830/149‑1 (MQ=255)
                                                                                          tggCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTgg                                            >  2:174758/1‑149 (MQ=255)
                                                                                              aGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAAt                                        >  2:168362/1‑149 (MQ=255)
                                                                                                   ggTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTg                                   <  2:822695/149‑1 (MQ=255)
                                                                                                    gTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGTCCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACGTGGGCCAGGTGTTTGAACATATTaaa                                                                    <  2:313296/115‑1 (MQ=255)
                                                                                                    gTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTATGAACATATTaaa                                                                    >  1:313296/1‑115 (MQ=255)
                                                                                                     tCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGtt                                 >  4:73478/1‑149 (MQ=255)
                                                                                                       aaaaGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCa                               <  4:272645/149‑1 (MQ=255)
                                                                                                        aaaGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGgtgt                                                                                  <  4:640828/97‑1 (MQ=255)
                                                                                                        aaaGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGgtgt                                                                                  >  3:640828/1‑97 (MQ=255)
                                                                                                         aaGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATc                             <  2:684744/149‑1 (MQ=255)
                                                                                                         aaGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATc                             >  2:496143/1‑149 (MQ=255)
                                                                                                          aGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCt                            <  1:465064/149‑1 (MQ=255)
                                                                                                              aTTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTaaaa                        >  3:539955/1‑149 (MQ=255)
                                                                                                              aTTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTaaaa                        >  1:136393/1‑149 (MQ=255)
                                                                                                               ttGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTaaaaa                       <  1:305450/149‑1 (MQ=255)
                                                                                                               ttGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTaaaaa                       >  1:849647/1‑149 (MQ=255)
                                                                                                                  aTGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAagta                    <  3:748918/149‑1 (MQ=255)
                                                                                                                   tGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAagtag                   >  1:899865/1‑149 (MQ=255)
                                                                                                                   tGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAagtag                   <  3:212156/149‑1 (MQ=255)
                                                                                                                     tAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTc                 >  2:918385/1‑149 (MQ=255)
                                                                                                                      gaaaGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCg                >  2:644416/2‑149 (MQ=255)
                                                                                                                      aaaaGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCg                >  2:782178/1‑149 (MQ=255)
                                                                                                                       aaaGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGc               >  3:153448/1‑149 (MQ=255)
                                                                                                                              aaCGCGAGGTCTTAGCCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCGGTaaa        >  3:468901/1‑149 (MQ=255)
                                                                                                                                   gAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCGGTAAATACCa   >  1:690463/1‑149 (MQ=255)
                                                                                                                                    aGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGCGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCGGTAAATACCAg  <  4:579352/149‑1 (MQ=255)
                                                                                                                                         tAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGt                                  >  1:374883/1‑113 (MQ=255)
                                                                                                                                         tAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGt                                  <  2:374883/113‑1 (MQ=255)
                                                                                                                                          aaGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCgg            <  2:896544/134‑1 (MQ=255)
                                                                                                                                          aaGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCgg            >  1:896544/1‑134 (MQ=255)
                                                                                                                                          |                                                                                                                                               
GTCAATGTACGCTATATGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCGGTAAATACCAG  >  NC_000913/1330262‑1330542

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: