Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,371,933 T→A 5.2% L9I (TTA→ATA)  ycjN → putative ABC sugar transporter periplasmic binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,371,9330TA 5.2% 414.9 / 8.1 116L9I (TTA→ATA) ycjNputative ABC sugar transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base T (52/58);  new base A (3/3);  total (55/61)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 117 total aligned reads displayed.

AGCATGTAAATATTACTAATCTGCATGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGAATGTATCCATCGAATTTATGCATTCTTCGGTGGAGCAGGAGCGCCAGGCCGTTATCAGTAAATTGATTGCCCGTTTTGAAAAAGAAAACCC  >  NC_000913/1371789‑1372081
                                                                                                                                                |                                                                                                                                                    
aGCATGTAAATATTACTAATCTGCATGGTCGGGATCCGATTAGTGAAGTTGATATATTGGGTAATGAAATAACACTACGCCCCTGGCAGGTTATGTAGATTAAATAAAAAGGAACATCTCAAGATTGAATCAAAAATCGTGCTGTTAtg                                                                                                                                                  >  2:673362/1‑148 (MQ=255)
 gCATGTAAATATTACTAATCTGCATGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCa                                                                                                                                                 >  4:751259/1‑149 (MQ=255)
  cATGTAAATATTACTAATCTGCATGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAg                                                                                                                                                <  4:674135/149‑1 (MQ=255)
      tAAATATTACTAATCTGCATGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACt                                                                                                                                            >  1:137394/1‑149 (MQ=255)
        aaTATTACTAATCTGCATGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTgg                                                                                                                                          >  4:836959/1‑149 (MQ=255)
          tatTACTAATCTGCATGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGtt                                                                                                                                        <  3:836959/149‑1 (MQ=255)
            ttACTAATCTGCATGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTc                                                                                                                                      <  2:255567/149‑1 (MQ=255)
              aCTAATCTGCATGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCAt                                                                                                                                    <  2:664227/149‑1 (MQ=255)
                tAATCTGCATGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATgc                                                                                                                                  >  4:708907/1‑149 (MQ=255)
                   tCTGCATGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGcc                                                                                                                               <  4:312829/149‑1 (MQ=255)
                       cATGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTAGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGa                                                                                                                           <  1:399517/149‑1 (MQ=255)
                        aTGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGAt                                                                                                                          <  4:484637/149‑1 (MQ=255)
                        aTGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGAt                                                                                                                          <  1:820538/149‑1 (MQ=255)
                             cGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAg                                                                                                                     <  2:333142/149‑1 (MQ=255)
                             cGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAg                                                                                                                     >  2:725659/1‑149 (MQ=255)
                                gATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCt                                                                                                                  >  1:245903/1‑149 (MQ=255)
                                      aTTAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCAt                                                                                                                                    >  4:143626/1‑125 (MQ=255)
                                      aTTAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCAt                                                                                                                                    <  3:143626/125‑1 (MQ=255)
                                      aTTAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAaag                                                                                                            >  3:424897/1‑149 (MQ=255)
                                        tAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAaagaa                                                                                                          <  3:751259/149‑1 (MQ=255)
                                        tAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAaagaa                                                                                                          <  3:708907/149‑1 (MQ=255)
                                          gTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCAt                                                                                                                                    >  4:315762/1‑121 (MQ=255)
                                          gTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCAt                                                                                                                                    <  3:315762/121‑1 (MQ=255)
                                          gTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAagaaga                                                                                                        >  2:574782/1‑149 (MQ=255)
                                             aaGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATgc                                                                                                                                  >  3:261977/1‑120 (MQ=255)
                                             aaGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATgc                                                                                                                                  <  4:261977/120‑1 (MQ=255)
                                                ttGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATaa                                                                                                  >  3:586744/1‑149 (MQ=255)
                                                  gATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATaaaa                                                                                                <  4:201720/149‑1 (MQ=255)
                                                  gATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATaaaa                                                                                                <  1:122601/149‑1 (MQ=255)
                                                        ttGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGAATg                                                                                          >  4:687491/1‑149 (MQ=255)
                                                          gggTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTa                                                                                                               >  1:82234/1‑126 (MQ=255)
                                                          gggTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTa                                                                                                               <  2:82234/126‑1 (MQ=255)
                                                          gggTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGAATGTa                                                                                        <  2:137394/149‑1 (MQ=255)
                                                          gggTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGAATGTa                                                                                        <  2:388023/149‑1 (MQ=255)
                                                            gTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGaa                                                                                            >  1:882853/1‑143 (MQ=255)
                                                            gTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGaa                                                                                            <  2:882853/143‑1 (MQ=255)
                                                               aTGAAATAACGCTGCGCCCCTGGCAGGCTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTACAGGCTGTAAAGAAGAACATAAAACGAATGTATCCAt                                                                                   >  1:526296/1‑149 (MQ=255)
                                                                tGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGAATGTATCCATc                                                                                  >  4:276926/1‑149 (MQ=255)
                                                                   aaTAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGAATGTATCCATCGaa                                                                               >  4:60814/1‑149 (MQ=255)
                                                                    aTAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGAATGTATCCATCGAAt                                                                              >  2:579172/1‑149 (MQ=255)
                                                                      aaCGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGAATGTATCCATCGAAttt                                                                            >  1:347210/1‑149 (MQ=255)
                                                                         gCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGAAt                                                                                           <  1:540010/131‑1 (MQ=255)
                                                                         gCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGAAt                                                                                           >  2:540010/1‑131 (MQ=255)
                                                                         gCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGAATGTATCCATCGAAt                                                                              >  4:491912/1‑144 (MQ=255)
                                                                         gCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGAATGTATCCATCGAAt                                                                              <  3:491912/144‑1 (MQ=255)
                                                                          cTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGAATGTATCCATCGAATTTATGc                                                                        <  2:294540/149‑1 (MQ=255)
                                                                           tGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGAATGTATCCATCGAATTTATGCa                                                                       >  1:386425/1‑149 (MQ=255)
                                                                               ccccTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGAATGTATCCATCGAATTTATGCAttct                                                                   >  1:790353/1‑149 (MQ=255)
                                                                               ccccTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGAATGTATCCATCGAATTTATGCAttct                                                                   >  4:450957/1‑149 (MQ=255)
                                                                                cccTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGAATGTATCCATCGAATTTATGCAttctt                                                                  <  2:386425/149‑1 (MQ=255)
                                                                                  cTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGAATGTATCCATCGAATTTATGCATTCTTCg                                                                >  2:713178/1‑149 (MQ=255)
                                                                                   tGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGAATGTATCCATCGAATTTATGCATTCTTCgg                                                               >  3:828744/1‑149 (MQ=255)
                                                                                    ggCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGAATGTATCCATCGAATTTATGCATTCTTCGGt                                                              <  1:574782/149‑1 (MQ=255)
                                                                                       aGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGt                                                                                                                >  3:590472/1‑96 (MQ=255)
                                                                                       aGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGt                                                                                                                <  4:590472/96‑1 (MQ=255)
                                                                                        ggTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGAATGTATCCATCGAATTTATGCATTCTTCGGTGGAg                                                          <  4:424897/149‑1 (MQ=255)
                                                                                          ttATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGAATGTATCCATCGAATTTATGCATTCTTCGGTGGAGCa                                                        <  1:713178/149‑1 (MQ=255)
                                                                                           tATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAGATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGAATGTATCCATCGAATTTATGCATTCTTCGGTGGAGCAg                                                       <  1:599835/149‑1 (MQ=255)
                                                                                             tgtgGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGAATGTATCCATCGAATTTATGCATTCTTCGGTGGAGCAGGa                                                     <  1:428485/149‑1 (MQ=255)
                                                                                                 gATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGAATGTATCCATCGAATTTATGCATTCTTCGGTGGAGCAGGAgcgc                                                 >  1:81385/1‑149 (MQ=255)
                                                                                                 gATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGAATGTATCCATCGAATTTATGCATTCTTCGGTGGAGCAGGAgcgc                                                 >  1:705840/1‑149 (MQ=255)
                                                                                                  aTTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGAATGTATCCATCGAATTTATGCATTCTTCGGTGGAGCAGGAGCGcc                                                >  4:614269/1‑149 (MQ=255)
                                                                                                  aTTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGAATGTATCCATCGAATTTATGCATTCTTCGGTGGAGCAGGAGCGcc                                                >  1:454071/1‑149 (MQ=255)
                                                                                                    taaataaaAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTCCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGAATGTATCCATCGAATTTATGCATTCTTCGGTGGAGCAGGAGCGCCAg                                              <  4:586127/149‑1 (MQ=255)
                                                                                                       ataaaAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGAATGTATCCATCGAATTTATGCATTCTTCGGTGGAGCAGGAGCGCCAGGcc                                           <  2:526296/149‑1 (MQ=255)
                                                                                                       ataaaAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGAATGTATCCATCGAATTTATGCATTCTTCGGTGGAGCAGGAGCGCCAGGcc                                           <  3:760893/149‑1 (MQ=255)
                                                                                                       ataaaAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGAATGTATCCATCGAATTTATGCATTCTTCGGTGGAGCAGGAGCGCCAGGcc                                           <  1:725659/149‑1 (MQ=255)
                                                                                                        taaaAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGAATGTATCCATCGAATTTATGCATTCTTCGGTGGAGCAGGAGCGCCAGGCCg                                          >  3:122007/1‑149 (MQ=255)
                                                                                                          aaaaGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGAATGTATCCATCGAATTTATGCATTCTTCGGTGGAGCAGGAGCGCCAGGCCGtt                                        <  1:415839/149‑1 (MQ=255)
                                                                                                          aaaaGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGAATGTATCCATCGAATTTATGCATTCTTCGGTGGAGCAGGAGCGCCAGGCCGtt                                        >  2:138265/1‑149 (MQ=255)
                                                                                                           aaaGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGAATGTATCCATCGAATTTATGCATTCTTCGGTGGAGCAGGAGCGCCAGGCCGTTa                                       <  1:550311/149‑1 (MQ=255)
                                                                                                            aaGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGAATGTATCCATCGAATTTATGCATTCTTCGGTGGAGCAGGAGCGCCAGGCCGTTAt                                      <  2:615833/149‑1 (MQ=255)
                                                                                                               gAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGAATGTATCCATCGAATTTATGCATTCTTCGGTGGAGCAGGAGCGCCAGGCCGTTATCAg                                   <  3:450957/149‑1 (MQ=255)
                                                                                                                aaCATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGAATGTATCCATCGAATTTATGCATTCTTCgg                                                               <  2:374915/120‑1 (MQ=255)
                                                                                                                      tcATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATaa                                                                                                  <  2:818173/79‑1 (MQ=255)
                                                                                                                      tcATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATaa                                                                                                  >  1:818173/1‑79 (MQ=255)
                                                                                                                      tcATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGAATGTATCCATCGAATTTATGCATTCTTCGGTGGAGCAGGAGCGCCAGGCCGTTATCAGTAAattg                            >  4:743301/1‑149 (MQ=255)
                                                                                                                       cATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGAATGTATCCATCGAATTTATTCATTCTTCGGTGGAGCAGGAGCGCCAGGCCGTTATCAGTAAattga                           >  1:234348/1‑149 (MQ=255)
                                                                                                                        aTGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGAATGTATCCATCGAATTTATGCATTCTTCGGTGGAGCAGGAGCGCCAGGCCGTTATCAGTAAattgat                          <  2:397425/149‑1 (MQ=255)
                                                                                                                          gATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGAATGTATCCATCGAATTTATGCATTCTTCGGTGGAGCAGGAGCGCCAGGCCGTTATCAGTAAattgat                          <  1:44820/147‑1 (MQ=255)
                                                                                                                          gATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGAATGTATCCATCGAATTTATGCATTCTTCGGTGGAGCAGGAGCGCCAGGCCGTTATCAGTAAattgat                          >  2:44820/1‑147 (MQ=255)
                                                                                                                           aTTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGAATGTATCCATCGAATTTATGCATTCTTCGGTGGAGCAGGAGCGCCAGGCCGTTATCAGTAAATTGATTGc                       <  1:841115/149‑1 (MQ=255)
                                                                                                                            ttAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGAATGTATCCATCGAATTTATGCATTCTTCGGTGGAGCAGGAGCGCCAGGCCGTTATCAGTAAATTGATTGcc                      >  2:606347/1‑149 (MQ=255)
                                                                                                                             tAAATCAAAAATCGTGCTGTTAACAGCACAGGTTTCATGCGCCCTGATTTCTGGCTGTACAGAAGAAAATAAATCGAATGTATCCATCGAATTTATGCATTCTTCGGTGGAGCAGGAGCGCCAGGCCGTTATCAGTAAATTGATTGccc                     <  1:673362/149‑1 (MQ=255)
                                                                                                                                aTCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGAATGTATCCATCGAATTTATGCATTCTTCGGTGGa                                                           >  2:580971/1‑108 (MQ=255)
                                                                                                                                aTCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGAATGTATCCATCGAATTTATGCATTCTTCGGTGGAGCAGGAGCGCCAGGCCGTTATCAGTAAATTGATTGCCCGtt                  >  2:620042/1‑149 (MQ=255)
                                                                                                                                 tCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGAATGTATCCATCGAATTTATGCATTCTTCGGTGGAGCAGGAGCGCCAGGCCGTTATCAGTAAATTGATTGCCCGttt                 <  4:586744/149‑1 (MQ=255)
                                                                                                                                 tCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGAATGTATCCATCGAATTTATGCATTCTTCGGTGGAGCAGGAGCGCCAGGCCGTTATCAGTAAATTGATTGCCCGttt                 >  3:185390/1‑149 (MQ=255)
                                                                                                                                 tCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGAATGTATCCATCGAATTTATGCATTCTTCGGTGGAGCAGGAGCGCCAGGCCGTTATCAGTAAATTGATTGCCCGttt                 <  2:234348/149‑1 (MQ=255)
                                                                                                                                   aaaaaTCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGAATGTATCCATCGAATTTATGCATTCTTCGGTGGAGCAGGAGCGCCAGGCCGTTATCAGTAAatt                             >  2:658279/1‑135 (MQ=255)
                                                                                                                                   aaaaaTCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGAATGTATCCATCGAATTTATGCATTCTTCGGTGGAGCAGGAGCGCCAGGCCGTTATCAGTAAatt                             <  1:658279/135‑1 (MQ=255)
                                                                                                                                   aaaaaTCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGAATGTATCCATCGAATTTATGCATTCTTCGGTGGAGCAGGAGCGCCAGGCCGTTATCAGTAAATTGATTGCCCGTTTTg               >  4:130815/1‑149 (MQ=255)
                                                                                                                                     aaccagTGCTGATAACAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGAATGTATCCATCGAATTTATGCATTCTTCGGTGGAGCAGGAGCGCCAGGCCGTTATCAGTAAATTGATTGCCCGttt                 >  3:300898/6‑145 (MQ=255)
                                                                                                                                      accagTGCTGATAACAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGAATGTATCCATCGAATTTATGCATTCTTCGGTGGAGCAGGAgc                                                   <  1:319907/106‑1 (MQ=255)
                                                                                                                                      accagTGCTGATAACAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGAATGTATCCATCGAATTTATGCATTCTTCGGTGGAGCAGGAGCGCCAGGCCGTTATCAGTAAATTGATTGCCCGTTTTGa              <  1:494914/143‑1 (MQ=255)
                                                                                                                                      accagTGCTGATAACAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGAATGTATCCATCGAATTTATGCATTCTTCGGTGGAGCAGGAGCGCCAGGCCGTTATCAGTAAATTGATTGCCCGTTTTGa              >  2:494914/5‑147 (MQ=255)
                                                                                                                                       aTCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGAATGTATCCATCGAATTTATGCATTCTTCGGTGGAGCAGGAGCGCCAGGCCGTTATCAGTAAATTGATTGCCCGTTTTGaaaa           <  4:122007/149‑1 (MQ=255)
                                                                                                                                        tCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGAATGTATCCATCGAATTTATGCATTCTTCGGTGGAGCAGGAGCGCCAGGCCGTTATCAGTAAATTGATTGCCCGTTTTGaaaaa          <  3:512188/149‑1 (MQ=255)
                                                                                                                                         cGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGAATGTATCCATCGAATTTATGCATTCTTCGGTGGAGCAGGAGCGCCAGGCCGTTATCAGTAAATTGATTGCCCGTTTTGAAAAAg         >  3:72763/1‑149 (MQ=255)
                                                                                                                                             cTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGAATGTATCCATCGAATTTATGCATTCTTCGGTGGAGCAGGAGCGCCAGGCCGTTATCAGTAAATTGATTGCCCGTTTTGAAAAAGaaaa     >  1:557784/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                                    
AGCATGTAAATATTACTAATCTGCATGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGAATGTATCCATCGAATTTATGCATTCTTCGGTGGAGCAGGAGCGCCAGGCCGTTATCAGTAAATTGATTGCCCGTTTTGAAAAAGAAAACCC  >  NC_000913/1371789‑1372081

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: