Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,388,285 A→G 12.8% intergenic (+24/+20) tyrR → / ← tpx aromatic amino acid biosynthesis and transport regulon transcriptional regulator; autorepressor; ATPase; phosphatase/lipid hydroperoxide peroxidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,388,2850AG12.8% 234.2 / 23.3 86intergenic (+24/+20)tyrR/tpxaromatic amino acid biosynthesis and transport regulon transcriptional regulator; autorepressor; ATPase; phosphatase/lipid hydroperoxide peroxidase
Reads supporting (aligned to +/- strand):  ref base A (42/33);  new base G (8/3);  total (50/36)
Fisher's exact test for biased strand distribution p-value = 3.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.04e-01

CCAGCTTTATCGCAATTATCCCAGCACGCGCAAACTGGCAAAACGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCGGTGGTGATTTCATCCACCAGCTGGCTGAAAATCACATTGTCATTTTCGTCAATAACCACAACGGCACGCGCTGCCAGACCTTTCAG  >  NC_000913/1388141‑1388433
                                                                                                                                                |                                                                                                                                                    
ccAGCTTTATCGCAATTATCCCAGCACGCGCAAACTGGCAAAACGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACAcc                                                                                                                                                  >  1:429662/1‑149 (MQ=255)
ccAGCTTTATCGCAATTATCCCAGCACGCGCAAACTGGCAAAACGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACAcc                                                                                                                                                  >  3:491449/1‑149 (MQ=255)
ccAGCTTTATCGCAATTATCCCAGCACGCGCAAACTGGCAAAACGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACAcc                                                                                                                                                  >  2:546331/1‑149 (MQ=255)
     tttATCGCAATTATCCCAGCACGCGCAAACTGGCAAAACGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGTTGCACCATCAg                                                                                                                                             <  1:506831/149‑1 (MQ=255)
     tttATCGCAATTATCCCAGCACGCGCAAACTGGCAAAACGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAg                                                                                                                                             >  1:333511/1‑149 (MQ=255)
         tCGCAATTATCCCAGCACGCGCAAACTGGCAAAACGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGTTGCACCATCAg                                                                                                                                             >  1:284178/1‑145 (MQ=255)
         tCGCAATTATCCCAGCACGCGCAAACTGGCAAAACGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGTTGCACCATCAg                                                                                                                                             <  2:284178/145‑1 (MQ=255)
         tCGCAATTATCCCAGCACGCGCAAACTGGCAAAACGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCat                                                                                                                                         >  1:158645/1‑149 (MQ=255)
         tCGCAATTATCCCAGCACACGCAAACTGGCAAAACGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTAGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTCAGGGTGCAAAACCATCAGGCat                                                                                                                                         >  4:177040/1‑149 (MQ=255)
           gCAATTATCCCAGCACGCGAAAACTGGCAAAACGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCatat                                                                                                                                       >  3:924581/1‑149 (MQ=255)
                tATCCCAGCACGCGCAAACTGGCAAAACGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAAt                                                                                                                                  <  4:491449/149‑1 (MQ=255)
                 aTCCCAGCACGCGCAAACTGGCAAAACGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAAtt                                                                                                                                 <  3:407603/149‑1 (MQ=255)
                    ccAGCACGCGCAAACTGGCAAAACGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATg                                                                                                                              >  4:795995/1‑149 (MQ=255)
                    ccAGCACGCGAAAACTGGCAAAACGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATg                                                                                                                              <  4:924581/149‑1 (MQ=255)
                     cAGCACGCGCAAACTGGCAAAACGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGc                                                                                                                             >  1:585439/1‑149 (MQ=255)
                          cgcgCAAACTGGCAAAACGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCa                                                                                                                        <  2:333511/149‑1 (MQ=255)
                                   tGGCAAAACGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCa                                                                                                                                              >  2:477101/1‑118 (MQ=255)
                                   tGGCAAAACGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCa                                                                                                                                              <  1:477101/118‑1 (MQ=255)
                                   tGGCAAAACGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCa                                                                                                               >  2:698618/1‑149 (MQ=255)
                                       aaaaCGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTaaa                                                                                                                                   >  4:879120/1‑125 (MQ=255)
                                       aaaaCGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTaaa                                                                                                                                   <  3:879120/125‑1 (MQ=255)
                                       aaaaCGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTAt                                                                                                                               >  1:539753/1‑129 (MQ=255)
                                       aaaaCGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTAt                                                                                                                               <  2:539753/129‑1 (MQ=255)
                                        aaaCGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAgct                                                                                                          >  4:314162/1‑149 (MQ=255)
                                        aaaCGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAgct                                                                                                          <  1:917518/149‑1 (MQ=255)
                                             tctcGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTc                                                                                                     >  3:318249/1‑149 (MQ=255)
                                               tcGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGt                                                                                                                      <  3:784799/130‑1 (MQ=255)
                                               tcGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGt                                                                                                   >  1:439617/1‑149 (MQ=255)
                                               tcGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGGCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCTACACCATCAGGCATATTAAATTATGCTTTCAGt                                                                                                                      >  4:784799/1‑130 (MQ=255)
                                                  gCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAAt                                                                                                >  1:292993/1‑149 (MQ=255)
                                                    gcttCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATcc                                                                                              <  1:417332/147‑1 (MQ=255)
                                                     tttCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCg                                                                                             <  4:461699/149‑1 (MQ=255)
                                                      ttCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCgg                                                                                            <  3:177888/149‑1 (MQ=255)
                                                         acaTACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTc                                                                                                     >  4:591134/1‑137 (MQ=255)
                                                         acaTACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTc                                                                                                     <  3:591134/137‑1 (MQ=255)
                                                           aTACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCa                                                                                                                        >  2:515650/1‑116 (MQ=255)
                                                           aTACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCa                                                                                                                        <  1:515650/116‑1 (MQ=255)
                                                           aTACCACGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCgg                                                                                       >  2:743656/1‑149 (MQ=255)
                                                             aCCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCggtg                                                                                     <  3:534964/149‑1 (MQ=255)
                                                              ccGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCggtgg                                                                                    >  1:750025/1‑149 (MQ=255)
                                                               cgcgATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCggtggt                                                                                   <  2:292993/149‑1 (MQ=255)
                                                                   aTTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAgctgc                                                                                                        <  2:689229/124‑1 (MQ=255)
                                                                   aTTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAgctgc                                                                                                        >  1:689229/1‑124 (MQ=255)
                                                                   aTTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCGGTGGTGAtt                                                                               <  3:177040/149‑1 (MQ=255)
                                                                        cAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATc                                                                                               <  2:677087/128‑1 (MQ=255)
                                                                        cAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATc                                                                                               >  1:677087/1‑128 (MQ=255)
                                                                        cAATAAGTTGCCGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCGGTGGTGATTTCATc                                                                          <  1:394476/149‑1 (MQ=255)
                                                                               ttGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCGGTGGTGATTTCATCCACCAgc                                                                   >  2:597663/1‑149 (MQ=255)
                                                                                tGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCGGTGGTGATTTCATCCACCAgct                                                                  <  2:299003/149‑1 (MQ=255)
                                                                                 gCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAgctg                                                                                                         <  2:758139/109‑1 (MQ=255)
                                                                                 gCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAgctg                                                                                                         >  1:758139/1‑109 (MQ=255)
                                                                                 gCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCGGTGGTGATTTCATCCACCAgctg                                                                 >  1:674430/1‑149 (MQ=255)
                                                                                     gAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCGGTGGTGATTTCATCCACCAgctggctg                                                             >  2:578101/1‑149 (MQ=255)
                                                                                         atGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCGGTGGTGATTTCATCCACCAGCTGGCTGaaaa                                                         <  2:439617/149‑1 (MQ=255)
                                                                                            gTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCGGTGGTGATTTCATCCACCAgctggct                                                              >  2:447199/1‑141 (MQ=255)
                                                                                            gTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCGGTGGTGATTTCATCCACCAgctggct                                                              <  1:447199/141‑1 (MQ=255)
                                                                                            gTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCGGTGGTGATTTCATCCACCAGCTGGCTGAAAATca                                                      <  2:750025/149‑1 (MQ=255)
                                                                                                    cAGAAGAAGAACGAAGAGTAAGCGCGGATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCGGTGGTGATTTCATCCACCAGCTGGCTGAAAATCACATTGTCa                                              <  2:158645/149‑1 (MQ=255)
                                                                                                         gaagaaCGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCGGTGGTGATTTCATCCACCAGCTGGCTGAAAATCACATTGTCATTTTc                                         >  1:564948/1‑149 (MQ=255)
                                                                                                           agaaCGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCGGTGGTGATTTCATCCACCAGCTGGCTGAAAATCACATTGTCATTTTCGt                                       <  1:320642/149‑1 (MQ=255)
                                                                                                            gaaCGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCGGTGGTGATTTCATCCACCAGCTGGCTGAAAATCACATTGTCATTTTCGTc                                      <  1:574941/149‑1 (MQ=255)
                                                                                                              aCGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCGGTGGTGATTTCATCCACCAGCTGGCTGAAAATCACATTGTCATTTTCGTCaa                                    >  2:382863/1‑149 (MQ=255)
                                                                                                                gAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCGGTGGTGATTTCATCCACCAGCTGGCTGAAAATCACATTGTCAtt                                            >  4:326405/1‑139 (MQ=255)
                                                                                                                gAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCGGTGGTGATTTCATCCACCAGCTGGCTGAAAATCACATTGTCAtt                                            <  3:326405/139‑1 (MQ=255)
                                                                                                                gAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCGGTGGTGATTTCATCCACCAGCTGGCTGAAAATCACATTGTCATTTTCGTCAATa                                  >  1:118152/1‑149 (MQ=255)
                                                                                                                   gagTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCGGTGGTGATTTCATCCACCAGCTGGCTGAAAATCACATTGTCATTTTCGTCAATAAcc                               <  1:215318/149‑1 (MQ=255)
                                                                                                                            cgAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCGGTGGTGATTTCATCCACCAGCTGGCTGAAAATCACATTGTCATTTTCGTCAATAACCACAACGGCa                      >  3:598571/1‑149 (MQ=255)
                                                                                                                             gAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCGGTGGTGATTTCATCCACCAGCTGGCTGAAAATCACATTGTCATTTTCGTCAATAACCACAACGGCAc                     >  1:606308/1‑149 (MQ=255)
                                                                                                                              aaTATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCGGTGGTGATTTCATCCACCAGCTGGCTGAAAATCACATTGTCATTTTCGTCAATAAc                                <  2:176531/137‑1 (MQ=255)
                                                                                                                              aaTATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCGGTGGTGATTTCATCCACCAGCTGGCTGAAAATCACATTGTCATTTTCGTCAATAAc                                >  1:176531/1‑137 (MQ=255)
                                                                                                                                    ccTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCGGTGGTGATTTCATCCACCAGCTGGCTGAAAATCACATTGTCATTTTCGTCAATAACCACAACGGCACGCGCTGc              <  2:361622/149‑1 (MQ=255)
                                                                                                                                     cTGATGGTGTTGCACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCGGTGGTGATTTCATCCACCAGCTGGCTGAAAATCACATTGTCATTTTCGTCAATAACCACAAc                          <  2:532125/136‑1 (MQ=255)
                                                                                                                                     cTGATGGTGTTGCACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCGGTGGTGATTTCATCCACCAGCTGGCTGAAAATCACATTGTCATTTTCGTCAATAACCACAAc                          >  1:532125/1‑136 (MQ=255)
                                                                                                                                     cTGATGGTGTTGCACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCGGTGGTGATTTCATCCACCAGCTGGCTGAAAATCACATTGTCATTTTCGTCAATAACCACAACGGCACGCGCTGcc             >  2:524199/1‑149 (MQ=255)
                                                                                                                                     cTGATGGTGTTGCACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCGGTGGTGATTTCATCCACCAGCTGGCTGAAAATCACATTGTCATTTTCGTCAATAACCACAACGGCACGCGCTGcc             >  1:401209/1‑149 (MQ=255)
                                                                                                                                     cTGATGGTGTTGCACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCGGTGGTGATTTCATCCACCAGCTGGCTGAAAATCACATTGTCATTTTCGTCAATAACCACAACGGCACGCGCTGcc             >  2:893971/1‑149 (MQ=255)
                                                                                                                                     cTGATGGTGTTGCACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCGGTGGTGATTTCATCCACCAGCTGGCTGAAAATCACATTGTCATTTTCGTCAATAACCACAACGGCACGCGCTGcc             >  2:194167/1‑149 (MQ=255)
                                                                                                                                     cTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCGGTGGTGATTTCATCCACCAGCTGGCTGAAAATCACATTGTCATTTTCGTCAATAACCACAACGGCACGCGCTGcc             >  2:314133/1‑149 (MQ=255)
                                                                                                                                     cTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCGGTGGTGATTTCATCCACCAGCTGGCTGAAAATCACATTGTCATTTTCGTCAATAACCACAACGGCACGCGCTGcc             >  3:533084/1‑149 (MQ=255)
                                                                                                                                      tGATGGTGTTGCACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCGGTGGTGATTTCATCCACCAGCTGGCTGAAAATCACATTGTCATTTTCGTCAATAACCACAACGGCACGCGCTGCCa            >  4:278826/1‑149 (MQ=255)
                                                                                                                                      tGATGGTGTTGCACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCGGTGGTGATTTCATCCACCAGCTGGCTGAAAATCACATTGTCATTTTCGTCAATAACCACAACGGCACGCGCTGCCa            >  1:891999/1‑149 (MQ=255)
                                                                                                                                            tGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCGGTGGTGATTTCATCCACCAGCTGGCTGAAAATCACATTGTCATTTTCGTCAATAACCACAACGGCACGCGCTGCCAGACCtt      >  2:24096/1‑149 (MQ=255)
                                                                                                                                             gCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGGGCTGCTTCGTAATCCGGCTCGGTGGTGATTTCATCCACCAGCTGGCTGAAAATCACATTGTCATTTTCGTCAATAACCACAACGGCACGCGCTGCCAGACCttt     >  2:788180/1‑149 (MQ=255)
                                                                                                                                             gCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCGGTGGTGATTTCATCCACCAGCTGGCTGAAAATCACATTGTCATTTTCGTCAATAACCACAACGGCACGCGCTGCCAGACCttt     <  1:382863/149‑1 (MQ=255)
                                                                                                                                             gCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCGGTGGTGATTTCATCCACCAGCTGGCTGAAAATCACATTGTCATTTTCGTCAATAACCACAACGGCACGCGCTGCCAGACCttt     >  1:112210/1‑149 (MQ=255)
                                                                                                                                              cAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCGGTGGTGATTTCATCCACCAGCTGGCTGAAAATCACATTGTCATTTTCGTCAATAACCACAACGGCACGCGCTGCCAGACCTTTc    >  3:875731/1‑149 (MQ=255)
                                                                                                                                               aaCACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCGGTGGTGATTTCATCCACCAGCTGGCTGAAAATCACATTGTCATTTTCGTCAATAACCACAACGGCACGCGCTGCCAGACCTTTCa   >  1:154928/1‑149 (MQ=255)
                                                                                                                                                acacCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCGGTGGTGATTTCATCCACCAGCTGGCTGAAAATCACATTGTCATTTTCGTCAATAACCACAACGGCACGCGCTGCCAGACCTTTCAg  >  3:898223/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                                    
CCAGCTTTATCGCAATTATCCCAGCACGCGCAAACTGGCAAAACGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCGGTGGTGATTTCATCCACCAGCTGGCTGAAAATCACATTGTCATTTTCGTCAATAACCACAACGGCACGCGCTGCCAGACCTTTCAG  >  NC_000913/1388141‑1388433

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: