Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,482,227 C→A 5.5% intergenic (+26/+28) ynbD → / ← azoR putative phosphatase inner membrane protein/NADH‑azoreductase, FMN‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,482,2270CA 5.5% 310.5 / 4.8 90intergenic (+26/+28)ynbD/azoRputative phosphatase inner membrane protein/NADH‑azoreductase, FMN‑dependent
Reads supporting (aligned to +/- strand):  ref base C (43/42);  new base A (3/2);  total (46/44)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01

TTGTTATGTTACGGACACTGTAAAACCGTTAATGAAGCGATTAGCTATATTCGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCATTTCCGGACCGTATGCGATCCCTTCGGCGAAGACAAATTTCACATCGGTAATGCCGATAAAG  >  NC_000913/1482079‑1482375
                                                                                                                                                    |                                                                                                                                                    
ttgttATGTTACGGACACTGTAAAACCGTTAATGAAGCGATTAGCTATATTCGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATc                                                                                                                                                      >  3:188642/1‑149 (MQ=255)
  gttatgttaCGGACACTGTAAAACCGTTAATGAAGCGATTAGCTATATTCGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTa                                                                                                                                                    <  2:587590/149‑1 (MQ=255)
   ttatgttaCGGACACTGTAAAACCGTTAATGAAGCGATTAGCTATATTCGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTAc                                                                                                                                                   >  3:424367/1‑149 (MQ=255)
       gttaCTGACACTGTAAAACCGTTAATGAAGCGATTAGCTATATTCGAGCCAGACGCCCGCAGAATGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAACACTAGTAAGTGGATTGAGATGTGGACTGTAGATATTCAGTc                                                                                                                                               <  4:151067/149‑1 (MQ=255)
       gttaCGGACACTGTAAAACCGTTAATGAAGCGATTAGCTATATTCGAGTCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTc                                                                                                                                               >  4:545786/1‑149 (MQ=255)
        ttaCGGACACTGTAAAACCGTTAATGAAGCGATTAGCTATATTCGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTcc                                                                                                                                              >  3:545897/1‑149 (MQ=255)
        ttaCGGACACTGTAAAACCGTTAATGAAGCGATTAGCTATATTCGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTcc                                                                                                                                              >  2:845746/1‑149 (MQ=255)
        ttaCGGACACTGTAAAACCGTTAATGAAGCGATTAGCTATATTCGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTcc                                                                                                                                              <  4:188642/149‑1 (MQ=255)
           cGGACACTGTAAAACCGTTAATGAAGCGATTAGCTATATTCGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGTAGATATTCAGTCcac                                                                                                                                            <  4:814609/148‑1 (MQ=255)
           cGGACACTGTAAAACCGTTAATGAAGCGATTAGCTATATTCGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGTAGATATTCAGTCcac                                                                                                                                            >  3:814609/1‑148 (MQ=255)
           cGGACACTGTAAAACCGTTAATGAAGCGATTAGCTATATTCGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCcaca                                                                                                                                           <  4:490277/149‑1 (MQ=255)
             gACACTGTAAAACCGTTAATGAAGCGATTAGCTATATTCGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATc                                                                                                                                         <  1:206021/149‑1 (MQ=255)
              acacTGTAAAACCGTTAATGAAGCGATTAGCTATATTCGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCa                                                                                                                                        >  2:833342/1‑149 (MQ=255)
              acacTGTAAAACCGTTAATGAAGCGATTAGCTATATTCGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCa                                                                                                                                        >  1:776884/1‑149 (MQ=255)
                 cTGTAAAACCGTTAATGAAGCGATTAGCTATATTCGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGAAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGa                                                                                                                                     <  3:528552/149‑1 (MQ=255)
                  tGTAAAACCGTTAATGAAGCGATTAGCTATATTCGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGAc                                                                                                                                    >  1:203195/1‑149 (MQ=255)
                  tGTAAAACCGTTAATGAAGCGATTAGCTATATTCGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGAc                                                                                                                                    <  2:776884/149‑1 (MQ=255)
                  tGTAAAACCGTTAATGAAGCGATTAGCTATATTCGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGAc                                                                                                                                    <  1:452130/149‑1 (MQ=255)
                   gTAAAACCGTTAATGAAGCGATTAGCTATATTCGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGAcc                                                                                                                                   >  4:342783/1‑149 (MQ=255)
                    tAAAACCGTTAATGAAGCGATTAGCTATATTCGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCg                                                                                                                                  >  3:570788/1‑149 (MQ=255)
                      aaaCCGTTAATGAAGCGATTAGCTATATTCGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCgtg                                                                                                                                >  3:747299/1‑149 (MQ=255)
                        aCCGTTAATGAAGCGATTAGCTATATTCGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTc                                                                                                                              <  4:747299/149‑1 (MQ=255)
                          cGTTAATGAAGCGATTAGCTATATTCGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCg                                                                                                                            >  1:46115/1‑149 (MQ=255)
                          cGTTAATGAAGCGATTAGCTATATTCGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATGGAGATGTGGACTGAATATCTACAGGCCACATCAAGACCGTGTCCg                                                                                                                            <  2:600295/149‑1 (MQ=255)
                           gTTAATGAAGCGATTAGCTATATTCGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCca                                                                                                                                             >  2:431073/1‑131 (MQ=255)
                           gTTAATGAAGCGATTAGCTATATTCGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCca                                                                                                                                             <  1:431073/131‑1 (MQ=255)
                           gTTAATGAAGCGATTAGCTATATTCGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCgg                                                                                                                           <  2:46115/149‑1 (MQ=255)
                           gTTAATGAAGCGATTAGCTATATTCGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGAAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCgg                                                                                                                           >  1:203777/1‑149 (MQ=255)
                              aaTGAAGCGATTAGCTATATTCGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTa                                                                                                                        <  4:562154/149‑1 (MQ=255)
                               aTGAAGCGATTAGCTATATTCGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTAt                                                                                                                       >  3:149443/1‑149 (MQ=255)
                                 gAAGCGATTAGCTATATTCGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTc                                                                                                                                               >  1:858210/1‑123 (MQ=255)
                                 gAAGCGATTAGCTATATTCGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTc                                                                                                                                               <  2:858210/123‑1 (MQ=255)
                                  aaGCGATTAGCTATATTCGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGAGCGTGTCCGGTTATGCa                                                                                                                    >  3:788443/1‑149 (MQ=255)
                                  aaGCGATTAGCTATATTCGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCa                                                                                                                    >  4:320024/1‑149 (MQ=255)
                                          aGCTATATTCGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAAt                                                                                                            <  3:311327/149‑1 (MQ=255)
                                            cTATATTCGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGc                                                                                                          <  4:590630/149‑1 (MQ=255)
                                            cTATATTCGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGc                                                                                                          >  2:90465/1‑149 (MQ=255)
                                             tataTTCGAGCCAGGCGCCCGCAGACTGTGCTGACAGACGGGCACAAAGCGATGCCGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTCCAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCt                                                                                                         <  2:724487/149‑1 (MQ=255)
                                             tataTTCGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGTACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCt                                                                                                         >  3:545346/1‑149 (MQ=255)
                                             tataTTCGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTc                                                                                                                                               <  3:298342/111‑1 (MQ=255)
                                             tataTTCGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCt                                                                                                         >  1:706399/1‑149 (MQ=255)
                                             tataTTCGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGAGTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTc                                                                                                                                               >  4:298342/1‑111 (MQ=255)
                                               tatTCGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGt                                                                                                       >  1:794465/1‑149 (MQ=255)
                                                atTCGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTc                                                                                                      <  2:794465/149‑1 (MQ=255)
                                                 ttCGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCg                                                                                                     >  3:715905/1‑149 (MQ=255)
                                                  tCGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGa                                                                                                    <  2:211165/149‑1 (MQ=255)
                                                    gAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATg                                                                                                  <  4:149443/149‑1 (MQ=255)
                                                             gCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCtttt                                                                                         <  3:320024/149‑1 (MQ=255)
                                                             gCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCtttt                                                                                         >  2:144000/1‑149 (MQ=255)
                                                               ccGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGc                                                                                       <  3:193119/149‑1 (MQ=255)
                                                               ccGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGc                                                                                       <  4:327737/149‑1 (MQ=255)
                                                               ccGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGc                                                                                       >  4:672169/1‑149 (MQ=255)
                                                                cGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCg                                                                                      >  1:481806/1‑149 (MQ=255)
                                                                  cAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTc                                                                                    <  4:545897/149‑1 (MQ=255)
                                                                     aTTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGa                                                                                 <  3:721722/149‑1 (MQ=255)
                                                                       tgtgCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACt                                                                               <  2:658841/149‑1 (MQ=255)
                                                                          gCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACtgtg                                                                            <  1:695500/149‑1 (MQ=255)
                                                                            tGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGTAGATATTCAGTcc                                                                                                                                              >  4:335677/1‑81 (MQ=38)
                                                                              aCAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCttt                                                                        <  1:833342/149‑1 (MQ=255)
                                                                                aGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCg                                                                      >  3:909471/1‑149 (MQ=255)
                                                                                aGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCg                                                                      >  1:548823/1‑149 (MQ=255)
                                                                                  aCGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCATGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTAGCt                                                                    <  4:788443/149‑1 (MQ=255)
                                                                                   cGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTg                                                                   <  4:381500/149‑1 (MQ=255)
                                                                                    gAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGc                                                                  >  2:709761/1‑149 (MQ=255)
                                                                                      gCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCaa                                                                                                                                       <  3:748814/78‑1 (MQ=255)
                                                                                      gCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCaa                                                                                                                                       >  4:748814/1‑78 (MQ=255)
                                                                                        acaAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAGACAATGCTGTCGACGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGACAtt                                                              >  4:12254/1‑149 (MQ=255)
                                                                                        acaAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGAATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCAtt                                                              >  4:763743/1‑149 (MQ=255)
                                                                                          aaaGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCATTTc                                                            <  3:342783/149‑1 (MQ=255)
                                                                                                    tGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATACAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCATTTCCGGACCGTAt                                                  >  4:548573/1‑149 (MQ=255)
                                                                                                        aTTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCATTTCCGGACCGTATGCGa                                              <  4:715905/149‑1 (MQ=255)
                                                                                                         ttATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCATTTCCGGACCGTATGCGAt                                             <  4:545346/149‑1 (MQ=255)
                                                                                                          tATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCATTTCCGGACCGTATGCGATc                                            <  2:706399/149‑1 (MQ=255)
                                                                                                            tGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCATTTCCGGACCGTATGCGATccc                                          <  4:909471/149‑1 (MQ=255)
                                                                                                            tGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCATTTCCGGACCGTATGCGATccc                                          <  3:548573/149‑1 (MQ=255)
                                                                                                               gAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCATTTCCGGACCGTATGCGATCCCTTc                                       >  1:356010/1‑149 (MQ=255)
                                                                                                                aaaaCAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCATTTCCGGACCGTATGCGATCCCTTCg                                      >  4:843387/1‑149 (MQ=255)
                                                                                                                  aaCAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCATTTCCGGACCGTATGCGATCCCTTCGGc                                    <  4:200983/149‑1 (MQ=255)
                                                                                                                    cAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCATTTCCGGACCGTATGCGATCCCTTCGGCGa                                  <  1:845746/149‑1 (MQ=255)
                                                                                                                          aGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCATTTCCGGACCGTATGCGATCCCTTCGGCGAAGACaa                            <  1:144000/149‑1 (MQ=255)
                                                                                                                                ttGCGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCATTTCCGGACCGTATGCGATCCCTTCGGCGAAGACAAATTTca                      <  3:263807/149‑1 (MQ=255)
                                                                                                                                       gtgGACTGTAGATATTCAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCATTTCCGGACCGTATGCGATCCCTTCGGCGAAGACAAATTTCACATCGGt               >  4:497646/1‑149 (MQ=255)
                                                                                                                                          gACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCATTTCCGGACCGTATGCGATCCCTTCGGCGAAGACAAATTTCACATCGGTAAt            >  3:103649/1‑149 (MQ=255)
                                                                                                                                          gACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCATTTCCGGACCGTATGCGATCCCTTCGGCGAAGACAAATTTCACATCGGTAAt            >  1:366516/1‑149 (MQ=255)
                                                                                                                                          gACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCATTTCCGGACCGTATGCGATCCCTTCGGCGAAGACAAATTTCACATCGGTAAt            >  4:80192/1‑149 (MQ=255)
                                                                                                                                           aCTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCATTTCCGGACCGTATGCGATCCCTTCGGCGAAGACAAATTTCACATCGGTAATg           >  1:721685/1‑149 (MQ=255)
                                                                                                                                            cTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCATTTCCGGACCGTATGCGATCCCTTCGGCGAAGACAAATTTCACATCGGTAATGc          <  3:12254/149‑1 (MQ=255)
                                                                                                                                             tGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTACGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCATTTCCGGACCGTATGCGATCCCTTCGGCGAAGACAAATTTCACATCGGTAATGcc         >  2:122893/1‑149 (MQ=255)
                                                                                                                                              gAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCATTTCCGGACCGTATGCGATCCCTTCGGCGAAGACAAATTTCACATCGGTAATGCCg        >  4:618011/1‑149 (MQ=255)
                                                                                                                                                atatCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCATTTCCGGACCGTATGCGATCCCTTCGGCGAAGACAAATTTCACATCGGTAATGCCGAt      >  3:761962/1‑149 (MQ=255)
                                                                                                                                                   tCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCATTTCCGGACCGTATGCGATCCCTTCGGCGAAGACAAATTTCACATCGGTAATGCCGATaaa   >  1:829062/1‑149 (MQ=255)
                                                                                                                                                    cTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCATGTCCGGACCGTATGCGATCCCTTCGGCGAAGACAAATTTCACATCGGTAATGCCGATAAAg  <  3:535321/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
TTGTTATGTTACGGACACTGTAAAACCGTTAATGAAGCGATTAGCTATATTCGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCATTTCCGGACCGTATGCGATCCCTTCGGCGAAGACAAATTTCACATCGGTAATGCCGATAAAG  >  NC_000913/1482079‑1482375

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: