Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,482,229 A→T 5.1% intergenic (+28/+26) ynbD → / ← azoR putative phosphatase inner membrane protein/NADH‑azoreductase, FMN‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,482,2290AT 5.1% 321.4 / 4.8 93intergenic (+28/+26)ynbD/azoRputative phosphatase inner membrane protein/NADH‑azoreductase, FMN‑dependent
Reads supporting (aligned to +/- strand):  ref base A (45/42);  new base T (3/2);  total (48/45)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.91e-01

GTTATGTTACGGACACTGTAAAACCGTTAATGAAGCGATTAGCTATATTCGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCATTTCCGGACCGTATGCGATCCCTTCGGCGAAGACAAATTTCACATCGGTAATGCCGATAAAGC  >  NC_000913/1482081‑1482376
                                                                                                                                                    |                                                                                                                                                   
gttatgttaCGGACACTGTAAAACCGTTAATGAAGCGATTAGCTATATTCGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTa                                                                                                                                                     <  2:587590/149‑1 (MQ=255)
 ttatgttaCGGACACTGTAAAACCGTTAATGAAGCGATTAGCTATATTCGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTAc                                                                                                                                                    >  3:424367/1‑149 (MQ=255)
     gttaCTGACACTGTAAAACCGTTAATGAAGCGATTAGCTATATTCGAGCCAGACGCCCGCAGAATGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAACACTAGTAAGTGGATTGAGATGTGGACTGTAGATATTCAGTc                                                                                                                                                <  4:151067/149‑1 (MQ=255)
     gttaCGGACACTGTAAAACCGTTAATGAAGCGATTAGCTATATTCGAGTCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTc                                                                                                                                                >  4:545786/1‑149 (MQ=255)
      ttaCGGACACTGTAAAACCGTTAATGAAGCGATTAGCTATATTCGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTcc                                                                                                                                               <  4:188642/149‑1 (MQ=255)
      ttaCGGACACTGTAAAACCGTTAATGAAGCGATTAGCTATATTCGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTcc                                                                                                                                               >  3:545897/1‑149 (MQ=255)
      ttaCGGACACTGTAAAACCGTTAATGAAGCGATTAGCTATATTCGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTcc                                                                                                                                               >  2:845746/1‑149 (MQ=255)
         cGGACACTGTAAAACCGTTAATGAAGCGATTAGCTATATTCGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGTAGATATTCAGTCcac                                                                                                                                             <  4:814609/148‑1 (MQ=255)
         cGGACACTGTAAAACCGTTAATGAAGCGATTAGCTATATTCGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGTAGATATTCAGTCcac                                                                                                                                             >  3:814609/1‑148 (MQ=255)
         cGGACACTGTAAAACCGTTAATGAAGCGATTAGCTATATTCGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCcaca                                                                                                                                            <  4:490277/149‑1 (MQ=255)
           gACACTGTAAAACCGTTAATGAAGCGATTAGCTATATTCGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATc                                                                                                                                          <  1:206021/149‑1 (MQ=255)
            acacTGTAAAACCGTTAATGAAGCGATTAGCTATATTCGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCa                                                                                                                                         >  2:833342/1‑149 (MQ=255)
            acacTGTAAAACCGTTAATGAAGCGATTAGCTATATTCGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCa                                                                                                                                         >  1:776884/1‑149 (MQ=255)
               cTGTAAAACCGTTAATGAAGCGATTAGCTATATTCGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGAAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGa                                                                                                                                      <  3:528552/149‑1 (MQ=255)
                tGTAAAACCGTTAATGAAGCGATTAGCTATATTCGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGAc                                                                                                                                     >  1:203195/1‑149 (MQ=255)
                tGTAAAACCGTTAATGAAGCGATTAGCTATATTCGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGAc                                                                                                                                     <  2:776884/149‑1 (MQ=255)
                tGTAAAACCGTTAATGAAGCGATTAGCTATATTCGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGAc                                                                                                                                     <  1:452130/149‑1 (MQ=255)
                 gTAAAACCGTTAATGAAGCGATTAGCTATATTCGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGAcc                                                                                                                                    >  4:342783/1‑149 (MQ=255)
                  tAAAACCGTTAATGAAGCGATTAGCTATATTCGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCg                                                                                                                                   >  3:570788/1‑149 (MQ=255)
                    aaaCCGTTAATGAAGCGATTAGCTATATTCGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCgtg                                                                                                                                 >  3:747299/1‑149 (MQ=255)
                      aCCGTTAATGAAGCGATTAGCTATATTCGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTc                                                                                                                               <  4:747299/149‑1 (MQ=255)
                        cGTTAATGAAGCGATTAGCTATATTCGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCg                                                                                                                             >  1:46115/1‑149 (MQ=255)
                        cGTTAATGAAGCGATTAGCTATATTCGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATGGAGATGTGGACTGAATATCTACAGGCCACATCAAGACCGTGTCCg                                                                                                                             <  2:600295/149‑1 (MQ=255)
                         gTTAATGAAGCGATTAGCTATATTCGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCca                                                                                                                                              <  1:431073/131‑1 (MQ=255)
                         gTTAATGAAGCGATTAGCTATATTCGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCca                                                                                                                                              >  2:431073/1‑131 (MQ=255)
                         gTTAATGAAGCGATTAGCTATATTCGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCgg                                                                                                                            <  2:46115/149‑1 (MQ=255)
                         gTTAATGAAGCGATTAGCTATATTCGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGAAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCgg                                                                                                                            >  1:203777/1‑149 (MQ=255)
                            aaTGAAGCGATTAGCTATATTCGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTa                                                                                                                         <  4:562154/149‑1 (MQ=255)
                             aTGAAGCGATTAGCTATATTCGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTAt                                                                                                                        >  3:149443/1‑149 (MQ=255)
                               gAAGCGATTAGCTATATTCGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTc                                                                                                                                                >  1:858210/1‑123 (MQ=255)
                               gAAGCGATTAGCTATATTCGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTc                                                                                                                                                <  2:858210/123‑1 (MQ=255)
                                aaGCGATTAGCTATATTCGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGAGCGTGTCCGGTTATGCa                                                                                                                     >  3:788443/1‑149 (MQ=255)
                                aaGCGATTAGCTATATTCGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCa                                                                                                                     >  4:320024/1‑149 (MQ=255)
                                        aGCTATATTCGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAAt                                                                                                             <  3:311327/149‑1 (MQ=255)
                                          cTATATTCGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGc                                                                                                           >  2:90465/1‑149 (MQ=255)
                                          cTATATTCGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGc                                                                                                           <  4:590630/149‑1 (MQ=255)
                                           tataTTCGAGCCAGGCGCCCGCAGACTGTGCTGACAGACGGGCACAAAGCGATGCCGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTCCAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCt                                                                                                          <  2:724487/149‑1 (MQ=255)
                                           tataTTCGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGTACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCt                                                                                                          >  3:545346/1‑149 (MQ=255)
                                           tataTTCGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTc                                                                                                                                                <  3:298342/111‑1 (MQ=255)
                                           tataTTCGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCt                                                                                                          >  1:706399/1‑149 (MQ=255)
                                           tataTTCGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGAGTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTc                                                                                                                                                >  4:298342/1‑111 (MQ=255)
                                             tatTCGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGt                                                                                                        >  1:794465/1‑149 (MQ=255)
                                              atTCGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTc                                                                                                       <  2:794465/149‑1 (MQ=255)
                                               ttCGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCg                                                                                                      >  3:715905/1‑149 (MQ=255)
                                                tCGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGa                                                                                                     <  2:211165/149‑1 (MQ=255)
                                                  gAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATg                                                                                                   <  4:149443/149‑1 (MQ=255)
                                                           gCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCtttt                                                                                          <  3:320024/149‑1 (MQ=255)
                                                           gCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCtttt                                                                                          >  2:144000/1‑149 (MQ=255)
                                                             ccGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGc                                                                                        <  3:193119/149‑1 (MQ=255)
                                                             ccGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGc                                                                                        >  4:672169/1‑149 (MQ=255)
                                                             ccGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGc                                                                                        <  4:327737/149‑1 (MQ=255)
                                                              cGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCg                                                                                       >  1:481806/1‑149 (MQ=255)
                                                                cAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTc                                                                                     <  4:545897/149‑1 (MQ=255)
                                                                   aTTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGa                                                                                  <  3:721722/149‑1 (MQ=255)
                                                                     tgtgCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACt                                                                                <  2:658841/149‑1 (MQ=255)
                                                                        gCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACtgtg                                                                             <  1:695500/149‑1 (MQ=255)
                                                                          tGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGTAGATATTCAGTcc                                                                                                                                               >  4:335677/1‑81 (MQ=38)
                                                                            aCAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCttt                                                                         <  1:833342/149‑1 (MQ=255)
                                                                              aGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCg                                                                       >  1:548823/1‑149 (MQ=255)
                                                                              aGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCg                                                                       >  3:909471/1‑149 (MQ=255)
                                                                                aCGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCATGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTAGCt                                                                     <  4:788443/149‑1 (MQ=255)
                                                                                 cGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTg                                                                    <  4:381500/149‑1 (MQ=255)
                                                                                  gAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGc                                                                   >  2:709761/1‑149 (MQ=255)
                                                                                    gCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCaa                                                                                                                                        >  4:748814/1‑78 (MQ=255)
                                                                                    gCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCaa                                                                                                                                        <  3:748814/78‑1 (MQ=255)
                                                                                      acaAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAGACAATGCTGTCGACGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGACAtt                                                               >  4:12254/1‑149 (MQ=255)
                                                                                      acaAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGAATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCAtt                                                               >  4:763743/1‑149 (MQ=255)
                                                                                        aaaGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCATTTc                                                             <  3:342783/149‑1 (MQ=255)
                                                                                                  tGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATACAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCATTTCCGGACCGTAt                                                   >  4:548573/1‑149 (MQ=255)
                                                                                                      aTTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCATTTCCGGACCGTATGCGa                                               <  4:715905/149‑1 (MQ=255)
                                                                                                       ttATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCATTTCCGGACCGTATGCGAt                                              <  4:545346/149‑1 (MQ=255)
                                                                                                        tATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCATTTCCGGACCGTATGCGATc                                             <  2:706399/149‑1 (MQ=255)
                                                                                                          tGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCATTTCCGGACCGTATGCGATccc                                           <  4:909471/149‑1 (MQ=255)
                                                                                                          tGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCATTTCCGGACCGTATGCGATccc                                           <  3:548573/149‑1 (MQ=255)
                                                                                                             gAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCATTTCCGGACCGTATGCGATCCCTTc                                        >  1:356010/1‑149 (MQ=255)
                                                                                                              aaaaCAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCATTTCCGGACCGTATGCGATCCCTTCg                                       >  4:843387/1‑149 (MQ=255)
                                                                                                                aaCAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCATTTCCGGACCGTATGCGATCCCTTCGGc                                     <  4:200983/149‑1 (MQ=255)
                                                                                                                  cAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCATTTCCGGACCGTATGCGATCCCTTCGGCGa                                   <  1:845746/149‑1 (MQ=255)
                                                                                                                        aGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCATTTCCGGACCGTATGCGATCCCTTCGGCGAAGACaa                             <  1:144000/149‑1 (MQ=255)
                                                                                                                              ttGCGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCATTTCCGGACCGTATGCGATCCCTTCGGCGAAGACAAATTTca                       <  3:263807/149‑1 (MQ=255)
                                                                                                                                     gtgGACTGTAGATATTCAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCATTTCCGGACCGTATGCGATCCCTTCGGCGAAGACAAATTTCACATCGGt                >  4:497646/1‑149 (MQ=255)
                                                                                                                                        gACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCATTTCCGGACCGTATGCGATCCCTTCGGCGAAGACAAATTTCACATCGGTAAt             >  1:366516/1‑149 (MQ=255)
                                                                                                                                        gACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCATTTCCGGACCGTATGCGATCCCTTCGGCGAAGACAAATTTCACATCGGTAAt             >  3:103649/1‑149 (MQ=255)
                                                                                                                                        gACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCATTTCCGGACCGTATGCGATCCCTTCGGCGAAGACAAATTTCACATCGGTAAt             >  4:80192/1‑149 (MQ=255)
                                                                                                                                         aCTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCATTTCCGGACCGTATGCGATCCCTTCGGCGAAGACAAATTTCACATCGGTAATg            >  1:721685/1‑149 (MQ=255)
                                                                                                                                          cTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCATTTCCGGACCGTATGCGATCCCTTCGGCGAAGACAAATTTCACATCGGTAATGc           <  3:12254/149‑1 (MQ=255)
                                                                                                                                           tGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTACGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCATTTCCGGACCGTATGCGATCCCTTCGGCGAAGACAAATTTCACATCGGTAATGcc          >  2:122893/1‑149 (MQ=255)
                                                                                                                                            gAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCATTTCCGGACCGTATGCGATCCCTTCGGCGAAGACAAATTTCACATCGGTAATGCCg         >  4:618011/1‑149 (MQ=255)
                                                                                                                                              atatCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCATTTCCGGACCGTATGCGATCCCTTCGGCGAAGACAAATTTCACATCGGTAATGCCGAt       >  3:761962/1‑149 (MQ=255)
                                                                                                                                                 tCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCATTTCCGGACCGTATGCGATCCCTTCGGCGAAGACAAATTTCACATCGGTAATGCCGATaaa    >  1:829062/1‑149 (MQ=255)
                                                                                                                                                  cTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCATGTCCGGACCGTATGCGATCCCTTCGGCGAAGACAAATTTCACATCGGTAATGCCGATAAAg   <  3:535321/149‑1 (MQ=255)
                                                                                                                                                   tACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGAGTGTGCTTTCGCTGCCATGTCCGGACCGTATGCGATCCCTTCGGCGAAGACAAATTTCACATCGGTAATGCCGATAAAGc  <  4:381870/149‑1 (MQ=255)
                                                                                                                                                   tACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCATTTCCGGACCGTATGCGATCCCTTCGGCGAAGACAAATTTCACATCGGTAATGCCGATAAAGc  >  1:751115/1‑149 (MQ=255)
                                                                                                                                                   tACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCATTTCCGGACCGTATGCGATCCCTTCGGCGAAGACAAATTTCACATCGGTAATGCCGATAAAGc  >  4:133554/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GTTATGTTACGGACACTGTAAAACCGTTAATGAAGCGATTAGCTATATTCGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCATTTCCGGACCGTATGCGATCCCTTCGGCGAAGACAAATTTCACATCGGTAATGCCGATAAAGC  >  NC_000913/1482081‑1482376

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: