Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,483,342 G→C 6.2% Q94H (CAG→CAC hrpA → putative ATP‑dependent helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,483,3420GC 6.2% 379.7 / 9.9 110Q94H (CAG→CAChrpAputative ATP‑dependent helicase
Reads supporting (aligned to +/- strand):  ref base G (55/48);  new base C (4/3);  total (59/51)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

Only 100 of 116 total aligned reads displayed.

CATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGCCATACCCAGCCGCGTCGTCTGGCGGCAAGAACAGTGGCGAACCGTATTGCG  >  NC_000913/1483195‑1483489
                                                                                                                                                   |                                                                                                                                                   
cATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAgg                                                                                                                                                    >  1:310407/1‑149 (MQ=255)
 aTTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAggt                                                                                                                                                   <  4:861224/149‑1 (MQ=255)
  ttttCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAggtg                                                                                                                                                  >  3:277139/1‑149 (MQ=255)
  ttttCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAggtg                                                                                                                                                  >  3:710316/1‑149 (MQ=255)
  ttttCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAggtg                                                                                                                                                  >  3:879737/1‑149 (MQ=255)
    ttCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAggtggt                                                                                                                                                <  1:826947/149‑1 (MQ=255)
      ccAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGa                                                                                                                                              <  1:609014/149‑1 (MQ=255)
      ccAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGa                                                                                                                                              <  2:180814/149‑1 (MQ=255)
       cAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGAt                                                                                                                                             <  1:79181/149‑1 (MQ=255)
        aGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGAtc                                                                                                                                            >  4:478031/1‑149 (MQ=255)
          gagaTGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCACCTGGTGAtcac                                                                                                                                          <  1:129718/149‑3 (MQ=255)
            gaTGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGAtcgtcg                                                                                                                                        >  1:412492/1‑149 (MQ=255)
             aTGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGc                                                                                                                                       <  1:600810/149‑1 (MQ=255)
               ggCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCg                                                                                                                                     <  1:556225/149‑1 (MQ=255)
                  gAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCgggg                                                                                                                                  <  3:752231/149‑1 (MQ=255)
                        gaTTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACgg                                                                                                                            <  3:661473/149‑1 (MQ=255)
                         aTTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACggg                                                                                                                           <  4:128595/149‑1 (MQ=255)
                         aTTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACggg                                                                                                                           <  2:310407/149‑1 (MQ=255)
                         aTTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCACCTGGTGAtca                                                                                                                                           >  4:189856/1‑132 (MQ=255)
                         aTTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATACTCGAAGCGATTCGTGATCACCACCTGGTGAtca                                                                                                                                           <  3:189856/133‑2 (MQ=255)
                          ttGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGt                                                                                                                          <  2:611382/149‑1 (MQ=255)
                            gACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTc                                                                                                                        <  4:879737/149‑1 (MQ=255)
                              ccAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTg                                                                                                                      >  1:151077/1‑149 (MQ=255)
                                aGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGt                                                                                                                    >  3:875860/1‑149 (MQ=255)
                                        gggAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGAc                                                                                                            >  2:785241/1‑149 (MQ=255)
                                        gggAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGAc                                                                                                            <  3:140234/149‑1 (MQ=255)
                                          gAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTc                                                                                                          >  1:417700/1‑149 (MQ=255)
                                          gAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTc                                                                                                          >  3:18873/1‑149 (MQ=255)
                                          gAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCCATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTc                                                                                                          >  3:875597/1‑149 (MQ=255)
                                           aaaGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCa                                                                                                         <  3:887719/149‑1 (MQ=255)
                                             aGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTgg                                                                                                                     <  1:135136/135‑1 (MQ=255)
                                             aGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTgg                                                                                                                     >  2:135136/1‑135 (MQ=255)
                                             aGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGt                                                                                                       <  2:417700/149‑1 (MQ=255)
                                              gTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGtt                                                                                                      >  3:244768/1‑149 (MQ=255)
                                               tCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTa                                                                                                     >  4:800848/1‑149 (MQ=255)
                                                 ctgctgCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTc                                                                                                                        >  1:635633/1‑128 (MQ=255)
                                                 ctgctgCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTc                                                                                                                        <  2:635633/128‑1 (MQ=255)
                                                     tgcGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGaaa                                                                                               >  4:658625/1‑149 (MQ=255)
                                                            aGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTa                                                                                        <  4:244768/149‑1 (MQ=255)
                                                             gCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGATAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTAt                                                                                       <  3:578665/149‑1 (MQ=255)
                                                             gCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTAt                                                                                       >  4:454386/1‑149 (MQ=255)
                                                                gCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGa                                                                                    >  4:188095/1‑149 (MQ=255)
                                                                    gACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTg                                                                                <  3:169103/149‑1 (MQ=255)
                                                                    gACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGGGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTg                                                                                <  4:809070/149‑1 (MQ=255)
                                                                       cGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAAt                                                                                             >  2:628970/1‑133 (MQ=255)
                                                                       cGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAAt                                                                                             <  1:628970/133‑1 (MQ=255)
                                                                       cGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTgggg                                                                             >  3:60947/1‑149 (MQ=255)
                                                                         gAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGgcg                                                                           >  2:845729/1‑149 (MQ=255)
                                                                         gAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGgcg                                                                           >  4:102802/1‑149 (MQ=255)
                                                                                 ttATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTa                                                                   >  1:765739/1‑149 (MQ=255)
                                                                                 ttATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTa                                                                   >  1:539666/1‑149 (MQ=255)
                                                                                   aTCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTaaa                                                                 <  2:412492/149‑1 (MQ=255)
                                                                                   aTCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTaaa                                                                 <  3:694349/149‑1 (MQ=255)
                                                                                   aTCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTaaa                                                                 >  1:430615/1‑149 (MQ=255)
                                                                                   aTCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACAGAAAATCTGTATGGAGCTGGGGCGCTGGATTaaa                                                                 <  4:277139/149‑1 (MQ=255)
                                                                                    tCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAg                                                                >  4:281519/1‑149 (MQ=255)
                                                                                      cTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGa                                                              >  3:320405/1‑149 (MQ=255)
                                                                                      cTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGa                                                              <  1:384697/149‑1 (MQ=255)
                                                                                      cTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGa                                                              <  4:18873/149‑1 (MQ=255)
                                                                                      cTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGa                                                              <  3:62712/149‑1 (MQ=255)
                                                                                      cTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGa                                                              <  1:172627/149‑1 (MQ=255)
                                                                                       tGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGt                                                                                         >  3:210478/1‑121 (MQ=255)
                                                                                       tGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGt                                                                                         >  3:334836/1‑121 (MQ=255)
                                                                                       tGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGt                                                                                         <  4:334836/121‑1 (MQ=255)
                                                                                             tttACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCg                                                       >  1:316827/1‑149 (MQ=255)
                                                                                               tACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGc                                                     <  4:320405/149‑1 (MQ=255)
                                                                                                  cGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGCCAt                                                  >  4:455200/1‑149 (MQ=255)
                                                                                                  cGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGCCAt                                                  >  1:225207/1‑149 (MQ=255)
                                                                                                     ttAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTaaa                                                                 <  3:778545/131‑1 (MQ=255)
                                                                                                     ttAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTaaa                                                                 >  4:778545/1‑131 (MQ=255)
                                                                                                         tCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGCCATACCCAGc                                           >  2:276691/1‑149 (MQ=255)
                                                                                                           aGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGCCATACCCAGCcg                                         >  2:637888/1‑149 (MQ=255)
                                                                                                            gAAAAAACAGCACCTTGTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGCCATACCCAGCcgc                                        >  4:578310/1‑149 (MQ=255)
                                                                                                                  aCAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTGTGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGCCATACCCAGCCGCgtcgtc                                  >  2:10305/1‑149 (MQ=255)
                                                                                                                  aCAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGCCATACCCAGCCGCgtcgtc                                  >  2:889464/1‑149 (MQ=255)
                                                                                                                  aCAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGCCATACCCAGCCGCgtcgtc                                  >  4:130140/1‑149 (MQ=255)
                                                                                                                     ggACATTCTCGAAGCGATTCGTGATCAACAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGCCATACCCAGCCGCGTCGTCTgg                               >  2:109751/1‑149 (MQ=255)
                                                                                                                      gACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGCCATACCCAGCCGCGTCGTCTggc                              >  4:886876/1‑149 (MQ=255)
                                                                                                                         aTTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACGGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGCCATACCCAGCCGCGTCGTCTggcggc                           >  2:546742/1‑149 (MQ=255)
                                                                                                                             tcGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGCCATACCCAGCCGCGTCGTCTGGCGGCAAGa                       >  2:436886/1‑149 (MQ=255)
                                                                                                                               gAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGCCATACCCAGCCGCGTCGTCTGGCGGCAAGAAc                     >  2:492479/1‑149 (MQ=255)
                                                                                                                               gAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGCCATACCCAGCCGCGTCGTCTGGCGGCAAGAAc                     >  2:274412/1‑149 (MQ=255)
                                                                                                                                aaGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGCCATACCCAGCCGCGTCGTCTGGCGGCAAGAACa                    >  1:838443/1‑149 (MQ=255)
                                                                                                                                aaGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGCCATACCCAGCCGCGTCGTCTGGCGGCAAGAACa                    <  2:838443/149‑1 (MQ=255)
                                                                                                                                  gCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGCCATACCCAGCCGCGTCGTCTGGCGGCAAGAACAGt                  <  1:845729/149‑1 (MQ=255)
                                                                                                                                  gCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGCCATACCCAACCGCGTCGTCTGGCGGCAAGAACAGt                  <  3:188095/149‑1 (MQ=255)
                                                                                                                                    gATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGCCATACCCAGCCGCGTCGTCTGGCGGCAAGAACAGTgg                >  4:185551/1‑149 (MQ=255)
                                                                                                                                     aTTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGCCATACCCAGCCGCGTCGTCTGGCGGCAAGAACAGTGGc               <  2:151077/149‑1 (MQ=255)
                                                                                                                                      ttCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGCCATACCCAGCCGCGTCGTCTGGCGGCAAGAACAGTGGCg              >  1:243456/1‑149 (MQ=255)
                                                                                                                                        gacgATCACCACCTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGCCATACCCAGCCGCGTCGTCTGGCGGCAAGAACAGTGGCGaa            >  1:904456/4‑149 (MQ=255)
                                                                                                                                         acgATCACCACCTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAg                                                                <  2:807867/94‑1 (MQ=255)
                                                                                                                                         acgATCACCACCTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAg                                                                >  1:807867/3‑96 (MQ=255)
                                                                                                                                          tGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGtt                                                                                                      <  4:585662/57‑1 (MQ=255)
                                                                                                                                          tGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGtt                                                                                                      >  3:585662/1‑57 (MQ=255)
                                                                                                                                           gATCACCACCTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGCCATACCCAGCCGCGTCGTCTGGCGGCAAGAACAGTGGCGAACCg         >  2:173309/1‑149 (MQ=255)
                                                                                                                                             tCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGCCATACCCAGCCGCGTCGTCTGGCGGCAAGAACAGTGGCGAACCGTa       <  3:880884/149‑1 (MQ=255)
                                                                                                                                             tCACCAGGTGGTGATCGCCGCCGGGGAAACTGGTGCTGGTAAAACGACGCCGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTAATCGGCCATACCCAGCCGCGTCGTCTGGCGGCAAGAACAGTGGCGAACCGTa       <  1:12219/149‑1 (MQ=255)
                                                                                                                                              cACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGCCATACCCAGCCGCGTCGTCTGGCGGCAAGAACAGTGGCGAACCGTAt      >  3:866919/1‑149 (MQ=255)
                                                                                                                                               aCCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGCCATACCCAGCCGCGTCGTCTGGCGGCAAGAACAGTGGCGAACCGTAtt     <  3:448028/149‑1 (MQ=255)
                                                                                                                                                  aGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGCCATACCCAGCCGCGTCGTCTGGCGGCAAGAACAGTGGCGAACCGTATTGCg  >  4:253006/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
CATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGCCATACCCAGCCGCGTCGTCTGGCGGCAAGAACAGTGGCGAACCGTATTGCG  >  NC_000913/1483195‑1483489

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: