Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,674,958 A→T 6.1% intergenic (+11/+14) tqsA → / ← pntB pheromone AI‑2 transporter/pyridine nucleotide transhydrogenase, beta subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,674,9580AT 6.1% 346.7 / 10.0 99intergenic (+11/+14)tqsA/pntBpheromone AI‑2 transporter/pyridine nucleotide transhydrogenase, beta subunit
Reads supporting (aligned to +/- strand):  ref base A (40/53);  new base T (3/3);  total (43/56)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.00e-01

Only 100 of 108 total aligned reads displayed.

TTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCGGGTTTTGCACACCAGCATAGCCAGTGTTCATCGAACGTTTAAAGACAATCACGTT  >  NC_000913/1674812‑1675106
                                                                                                                                                  |                                                                                                                                                    
tttttGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCaga                                                                                                                                                    <  2:283414/149‑1 (MQ=255)
 ttttGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCagag                                                                                                                                                   <  4:476207/149‑1 (MQ=255)
 ttttGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCagag                                                                                                                                                   <  4:166411/149‑1 (MQ=255)
    tGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCTGCTGAGGcc                                                                                                                                                <  4:194950/149‑1 (MQ=255)
       ggATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCTGCTGAGGCCGTc                                                                                                                                             <  3:734659/149‑1 (MQ=255)
       ggATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTc                                                                                                                                             <  2:26283/149‑1 (MQ=255)
        gATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCTGCTGAGGCCGTCa                                                                                                                                            <  3:239677/149‑1 (MQ=255)
        gATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCa                                                                                                                                            >  4:909302/1‑149 (MQ=255)
         aTGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAg                                                                                                                                           >  1:229365/1‑149 (MQ=255)
          tGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAgg                                                                                                                                          >  3:384932/1‑149 (MQ=255)
          tGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAgg                                                                                                                                          <  4:32255/149‑1 (MQ=255)
            gttgttAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGt                                                                                                                                        <  4:444338/149‑1 (MQ=255)
             ttgttAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGtt                                                                                                                                       >  1:880260/1‑149 (MQ=255)
               gttAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTAc                                                                                                                                     >  2:489146/1‑149 (MQ=255)
                 tAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACag                                                                                                                                   >  4:161846/1‑149 (MQ=255)
                  aGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACaga                                                                                                                                  <  1:593622/149‑1 (MQ=255)
                   ggACCGGGGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACagag                                                                                                                                 >  4:405613/1‑149 (MQ=255)
                    gACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGc                                                                                                                                >  1:463495/1‑149 (MQ=255)
                       cGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCttt                                                                                                                             <  2:240390/149‑1 (MQ=255)
                         gTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCa                                                                                                                           >  4:455457/1‑149 (MQ=255)
                              tATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGAtt                                                                                                                      <  4:105821/149‑1 (MQ=255)
                              tATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGAtt                                                                                                                      <  4:814241/149‑1 (MQ=255)
                                 gctgctTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCa                                                                                                                   <  1:404925/149‑1 (MQ=255)
                                           gTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTcagca                                                                                                                                                      <  3:167429/104‑1 (MQ=255)
                                           gTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTcagca                                                                                                                                                      >  4:167429/1‑104 (MQ=255)
                                           gTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTgg                                                                                                         >  4:868057/1‑149 (MQ=255)
                                                gTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTg                                                                                                    <  3:695616/149‑1 (MQ=255)
                                                 ttGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTgg                                                                                                   >  1:81766/1‑149 (MQ=255)
                                                  tGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGc                                                                                                  <  2:445302/149‑1 (MQ=255)
                                                     cAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTc                                                                                               <  2:55395/149‑1 (MQ=255)
                                                      aaTTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCa                                                                                              >  4:243514/1‑149 (MQ=255)
                                                          attGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCaa                                                                                          >  2:934015/1‑149 (MQ=255)
                                                              tCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAg                                                                                      <  4:870824/149‑1 (MQ=255)
                                                               cAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGc                                                                                     <  3:561627/149‑1 (MQ=255)
                                                               cAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGCCACCAAACAGc                                                                                     <  1:489146/149‑1 (MQ=255)
                                                                aaaaTTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATc                                                                                                                 >  2:735613/1‑120 (MQ=255)
                                                                aaaaTTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATc                                                                                                                 <  1:735613/120‑1 (MQ=255)
                                                                     tGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTgg                                                                               <  4:288153/149‑1 (MQ=255)
                                                                       cgcTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGgt                                                                             <  4:47627/149‑1 (MQ=255)
                                                                        gcTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTgg                                                                               <  4:727222/146‑1 (MQ=255)
                                                                        gcTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTgg                                                                               >  3:727222/1‑146 (MQ=255)
                                                                          ttGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGtt                                                                          >  4:19073/1‑149 (MQ=255)
                                                                          ttGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGtt                                                                          <  3:86832/149‑1 (MQ=255)
                                                                          ttGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGtt                                                                          >  3:238497/1‑149 (MQ=255)
                                                                           tGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGttt                                                                         <  3:725404/149‑1 (MQ=255)
                                                                              acaaacaGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTcc                                                                      <  3:51279/149‑1 (MQ=255)
                                                                                aaacaGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCtt                                                                    >  1:926032/1‑149 (MQ=255)
                                                                                aaacaGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCtt                                                                    >  3:452038/1‑149 (MQ=255)
                                                                                   caGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTgaa                                                                 <  2:720383/149‑1 (MQ=255)
                                                                                       gggAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGCGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAAc                                                             <  3:300860/149‑1 (MQ=255)
                                                                                        ggAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACa                                                            <  3:19073/149‑1 (MQ=255)
                                                                                           ggTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCg                                                         <  2:199928/149‑1 (MQ=255)
                                                                                            gTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCACAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCgg                                                        <  1:692349/149‑1 (MQ=255)
                                                                                             tCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCggg                                                       <  4:184767/149‑1 (MQ=255)
                                                                                             tCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCggg                                                       >  1:177098/1‑149 (MQ=255)
                                                                                             tCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCggg                                                       >  2:17734/1‑149 (MQ=255)
                                                                                               aaaGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGc                                                                                                  <  3:427937/104‑1 (MQ=255)
                                                                                               aaaGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGc                                                                                                  >  4:427937/1‑104 (MQ=255)
                                                                                               aaaGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCGGGtt                                                     >  3:218391/1‑149 (MQ=255)
                                                                                               aaaGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCGGGtt                                                     <  2:880178/149‑1 (MQ=255)
                                                                                                   cATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCGGGTTTTGc                                                 >  2:714645/1‑149 (MQ=255)
                                                                                                          gTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCGGGTTTTGCACACCAg                                          >  4:732941/1‑149 (MQ=255)
                                                                                                          gTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCGGGTTTTGCACACCAg                                          <  2:894829/149‑1 (MQ=255)
                                                                                                           ttCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTcc                                                                      >  3:33129/1‑120 (MQ=255)
                                                                                                           ttCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTcc                                                                      <  4:33129/120‑1 (MQ=255)
                                                                                                             cTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCGGGTTTTGCACACCAGCAt                                       <  4:218391/149‑1 (MQ=255)
                                                                                                              tGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCGGGTTTTGCACACCAGCATa                                      <  4:819191/149‑1 (MQ=255)
                                                                                                                  aaGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCGGGTTTTGCACACCAGCATAGCCa                                  <  4:268410/149‑1 (MQ=255)
                                                                                                                  aaGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCGGGTTTTGCACACCAGCATAGCCa                                  >  2:299530/1‑149 (MQ=255)
                                                                                                                  aaGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCGGGTTTTGCACACCAGCATAGCCa                                  <  3:868057/149‑1 (MQ=255)
                                                                                                                  aaGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCGGGTTTTGCACACCAGCATAGCCa                                  >  3:659825/1‑149 (MQ=255)
                                                                                                                   aGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCGGGTTTTGCACACCAGCATAGCCAg                                 >  4:739349/1‑149 (MQ=255)
                                                                                                                    gCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCGGGTTTTGCACACCAGCATAGCCAgt                                <  4:452038/149‑1 (MQ=255)
                                                                                                                    gCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCGGGTTTTGCACACCAGCATAGCCAgt                                <  4:384932/149‑1 (MQ=255)
                                                                                                                    gCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCGGGTTTTGCACACCAGCATAGCCAgt                                >  4:805057/1‑149 (MQ=255)
                                                                                                                    gCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCGGGTTTTGCACACCAGCATAGCCAgt                                <  1:876511/149‑1 (MQ=255)
                                                                                                                      gATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCGGGTTTTGCACACCAGCATAGCCAgtgt                              <  3:739349/149‑1 (MQ=255)
                                                                                                                         ctcAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCGGGTTTTGCACACCAGCATAGCCAGTGTTCa                           <  3:304954/149‑1 (MQ=255)
                                                                                                                          tcAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCGGGTTTTGCACACCAGCATAGCCAGTGTTCAt                          >  4:771033/1‑149 (MQ=255)
                                                                                                                            aaTAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCGGGTTTTGCACACCAGCATAGCCAGTGTTCATc                         <  4:672244/148‑1 (MQ=255)
                                                                                                                            aaTAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCGGGTTTTGCACACCAGCATAGCCAGTGTTCATc                         >  3:672244/1‑148 (MQ=255)
                                                                                                                            aaTAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCGGGTTTTGCACACCAGCATAGCCAGTGTTCATCg                        >  4:430386/1‑149 (MQ=255)
                                                                                                                            aaTAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCGGGTTTTGCACACCAGCATAGCCAGTGTTCATCg                        >  3:82649/1‑149 (MQ=255)
                                                                                                                             aTAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCGGGTTTTGCACACCAGCATAGCCAGTGTTCATCGa                       >  2:139984/1‑149 (MQ=255)
                                                                                                                               aaaGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCGGGTTTTGCACACCAGCATAGCCAGTGTTCATCGAAc                     <  2:81766/149‑1 (MQ=255)
                                                                                                                                     tGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCGGGTTTTGCACACCAGCATAGCCAGTGTTCATCGAACGTTTaa               <  4:749323/149‑1 (MQ=255)
                                                                                                                                      gACGGCCTCTGCTGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCGGGTTTTGCACACCAGCATAGCCAGTGTTCATCGAACGTTTaaa              >  1:525450/1‑149 (MQ=255)
                                                                                                                                       aCGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCGGGTTTTGCACACCAGCATAGCCAGTGTTCATCGAACGTTTAAAg             <  1:282096/149‑1 (MQ=255)
                                                                                                                                        cGGCCTCTGCTGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCGGGTTTTGCACACCAGCATAGCCAGTGTTCATCGAACGTTTAAAGa            >  2:646623/1‑149 (MQ=255)
                                                                                                                                          gCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCGGGTTTTGCACACCAGCATAGCCAGTGTTCATCGAACGTTTAAAGACa          >  3:737443/1‑149 (MQ=255)
                                                                                                                                           ccTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCGGGTTTTGCACACCAGCATAGCCAGTGTTCATCGAACGTTTAAAGACaa         <  2:662428/149‑1 (MQ=255)
                                                                                                                                           ccTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCGGGTTTTGCACACCAGCATAGCCAGTGTTCATCGAACGTTTAAAGACaa         <  2:154039/149‑1 (MQ=255)
                                                                                                                                           ccTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCGGGTTTTGCACACCAGCATAGCCAGTGTTCATCGAACGTTTAAAGACaa         <  2:229365/149‑1 (MQ=255)
                                                                                                                                            cTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCGGGTTTTGCACACCAGCATAGc                                    >  2:289327/1‑121 (MQ=255)
                                                                                                                                            cTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCGGGTTTTGCACACCAGCATAGc                                    <  1:289327/121‑1 (MQ=255)
                                                                                                                                            cTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCGGGTTTTGCACACCAGCATAGCCAGTGTTCATCGAACGTTTAAAGACAAt        <  1:934015/149‑1 (MQ=255)
                                                                                                                                             tCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCGGGTTTTGCACACCAGCATAGCCAGTGTTCATCGAACGTTTAAAGACAATc       >  1:375899/1‑149 (MQ=255)
                                                                                                                                                gcagAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCGGGTTTTGCACACCAGCATAGCCAGTGTTCATCGAACGTTTAAAGACAATCACg    >  3:595609/1‑149 (MQ=255)
                                                                                                                                                  agagGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCGGGTTTTGCACACCAGCATAGCCAGTGTTCATCGAACGTTTAAAGACAATCACGtt  <  2:177098/149‑1 (MQ=255)
                                                                                                                                                  agagGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCGGGTTTTGCACACCAGCATAGCCAGTGTTCATCGAACGTTTAAAGACAATCACGtt  >  2:460635/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
TTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCGGGTTTTGCACACCAGCATAGCCAGTGTTCATCGAACGTTTAAAGACAATCACGTT  >  NC_000913/1674812‑1675106

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: