BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,692,978	A→C	5.6%	L429R (CTC→CGC)	uidB ←	glucuronide transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,692,978	0	A	C	5.6%	353.6 / 8.4	103	L429R (CTC→CGC)	uidB	glucuronide transporter
Reads supporting (aligned to +/- strand): ref base A (49/48); new base C (3/3); total (52/51)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 104 total aligned reads displayed.

CCATGGCCACTATTTTTCTCATAGTTGCACCTTTGATGTTCTGATTATTTTTATTGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGATGCCCATAATAACTTCCGGCGTTTGCACCTGATTGGCGATATATCCGCTTAACCCCAAAATAAAGGCAGGAATT  >  NC_000913/1692830‑1693124

ccATGGCCACTATTTTTCTCATAGTCGCACCTTTGATGTTCTTATTATTTTTATTGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGa                                                                                                                                                    <  3:120968/149‑1 (MQ=255)

  aTGGCCACTATTTTTCTCATAGTTGCACCTTTGATGTTCTGATTATTTTTATTGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGc                                                                                                                                                  <  1:204347/149‑1 (MQ=255)

     gCCACTATTTTTCTCATAGTTGCACCTTTGATGTTCTGATTATTTTTATTGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGa                                                                                                                                               <  2:326332/149‑1 (MQ=255)

       cACTATTTTTCTCATAGTTGCCCCTTTGATGTTCTGATTATTTTTATTGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTGTTTACAATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATa                                                                                                                                             >  4:193411/1‑149 (MQ=255)

       cACTATTTTTCTCATAGTTGCACCTTTGATGTTCTGATTATTTTTATTGAATATTAATTAGTGATAACGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATa                                                                                                                                             >  2:590051/1‑149 (MQ=255)

        aCTATTTTTCTCATAGTTGCACCTTTGATGTTCTGATTATTTTTATTGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGACTTTTTTATCCGTGAGCGGATaa                                                                                                                                            >  1:388308/1‑149 (MQ=255)

          tATTTTTCTCATAGTTGCACCTTTGATGTTCTGATTATTTTTATTGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATaaaa                                                                                                                                          <  1:763610/149‑1 (MQ=255)

          tATTTTTCTCATAGTTGCACCTTTGATGTTCTGATTATTTTTATTGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATaaaa                                                                                                                                          >  1:6638/1‑149 (MQ=255)

          tATTTTTCTCATAGTTGCACCTTTGATGTTCTGATTATTTTTATTGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATaaaa                                                                                                                                          <  2:527321/149‑1 (MQ=255)

          tATTTTTCTCATAGTTGCACCTTTGATGTTCTGATTATTTTTATTGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGCTCACGGATaaaa                                                                                                                                          >  4:189315/1‑149 (MQ=255)

           aTTTTTCTCATAGTTGCACCTTTGATGTTCTGATTATTTTTATTGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAAc                                                                                                                                         <  1:775113/149‑1 (MQ=255)

            tttttCTCATAGTTGCACCTTTGATGTTCTGATTATTTTTATTGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAAcc                                                                                                                                        <  2:414235/149‑1 (MQ=255)

            tttttCTCATAGTTGCACCTTTGATGTTCTGATTATTTTTATTGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGCTCACGGATaaaaaa                                                                                                                                        <  3:181336/149‑3 (MQ=255)

            tttttCTCATAGTTGCACCTTTGATGTTCTGATTATTTTTATTGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGCTCACGGATaaaaaa                                                                                                                                        <  3:189315/149‑3 (MQ=255)

             ttttCTCATAGTTGCACCTTTGATGTTCTGATTATTTTTATTGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCa                                                                                                                                       <  2:474026/149‑1 (MQ=255)

              tttCTCATAGTTGCACCTTTGATGTTCTGATTATTTTTATTGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAg                                                                                                                                      >  1:876194/1‑149 (MQ=255)

               ttCTCATAGTTGCACCTTTGATGTTCTGATTATTTTTATTGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGa                                                                                                                                     <  3:148714/149‑1 (MQ=255)

                  tcATAGTTGCACCTTTGATGTTCTGATTATTTTTATTGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGataa                                                                                                                                  >  2:466147/1‑149 (MQ=255)

                     tAGTTGCACCTTTGATGTTCTGATTATTTTTATTGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGAtaataa                                                                                                                               <  4:58263/149‑1 (MQ=255)

                         tGCACCTTTGATGTTCTGATTATTTTTATTGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGaa                                                                                                                           <  2:92429/149‑1 (MQ=255)

                          gCACCTTTGATGTTCTGATTATTTTTATTGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAAt                                                                                                                          >  2:461541/1‑149 (MQ=255)

                           cACCTTTGATGTTCTGATTATTTTTATTGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATg                                                                                                                         <  1:466147/149‑1 (MQ=255)

                               tttGATGTTCTGATTATTTTTATTGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAg                                                                                                                     >  2:107297/1‑149 (MQ=255)

                               tttGATGTTCTGATTATTTTTATTGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAg                                                                                                                     >  2:17741/1‑149 (MQ=255)

                                  gATGTTCTGATTATTTTTATTGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTATTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAg                                                                                                                  >  1:163124/1‑149 (MQ=255)

                                   aTGTTCTGATTATTTTTATTGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGc                                                                                                                 <  3:184421/149‑1 (MQ=255)

                                   aTGTTCTGATTATTTTTATTGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGc                                                                                                                 <  4:267131/149‑1 (MQ=255)

                                     gTTCTGATTATTTTTATTGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTa                                                                                                                   <  3:881859/145‑1 (MQ=255)

                                     gTTCTGATTATTTTTATTGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTa                                                                                                                   >  4:881859/1‑145 (MQ=255)

                                      ttCTGATTATTTTTATTGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTa                                                                                                                   >  2:127080/1‑144 (MQ=255)

                                      ttCTGATTATTTTTATTGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTa                                                                                                                   <  1:127080/144‑1 (MQ=255)

                                      ttCTGATTATTTTTATTGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATa                                                                                                              >  1:487750/1‑149 (MQ=255)

                                         tGATTATTTTTATTGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAAt                                                                                                           >  3:366715/1‑149 (MQ=255)

                                             tattTTTATTGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGc                                                                                                       <  2:854487/149‑1 (MQ=255)

                                                ttttATTGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAg                                                                                                    >  4:681387/1‑149 (MQ=255)

                                                 tttATTGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAgg                                                                                                   <  2:163124/149‑1 (MQ=255)

                                                     ttGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAAc                                                                                                                              <  4:815900/118‑1 (MQ=255)

                                                     ttGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAAc                                                                                                                              >  3:815900/1‑118 (MQ=255)

                                                     ttGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACt                                                                                               >  1:391969/1‑149 (MQ=255)

                                                     ttGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACt                                                                                               <  1:639659/149‑1 (MQ=255)

                                                        aaTATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTCGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAg                                                                                            <  1:894201/149‑1 (MQ=255)

                                                             taattaGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAAt                                                                                       <  3:681387/149‑1 (MQ=255)

                                                              aattaGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAAtt                                                                                      <  2:583979/149‑1 (MQ=255)

                                                              aattaGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAAtt                                                                                      <  2:711659/149‑1 (MQ=255)

                                                                  aGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGAtg                                                                                  <  1:17741/149‑1 (MQ=255)

                                                                   gTGATATCGCTGATTAATTGCTGCTGCCCTTTTTTACGATTATCACTTTCAACCACGGTTTCTTTGACTTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATAACAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGAtgt                                                                                 >  4:299808/1‑149 (MQ=255)

                                                                         tCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGc                                                                                                                        <  3:692345/104‑1 (MQ=255)

                                                                         tCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGc                                                                                                                        >  4:692345/1‑104 (MQ=255)

                                                                         tCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGAt                                                                           >  2:781007/1‑149 (MQ=255)

                                                                           gCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGaa                                                                                                                           >  1:242854/1‑99 (MQ=255)

                                                                           gCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGaa                                                                                                                           <  2:242854/99‑1 (MQ=255)

                                                                           gCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGATGc                                                                         <  4:188253/149‑1 (MQ=255)

                                                                             tGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGATGccc                                                                       >  4:528503/1‑149 (MQ=255)

                                                                                   aTTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGATGCCCataata                                                                 >  4:902502/1‑149 (MQ=255)

                                                                                   aTTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGATGCCCataata                                                                 >  2:512897/1‑149 (MQ=255)

                                                                                        tgctgcACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGATGCCCATAATAACTTc                                                            <  3:528503/149‑1 (MQ=255)

                                                                                        tgctgcACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGATGCCCATAATAACTTc                                                            <  3:491712/149‑1 (MQ=255)

                                                                                           tgcACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTaa                                                                                             <  2:863162/113‑1 (MQ=255)

                                                                                           tgcACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTaa                                                                                             >  1:863162/1‑113 (MQ=255)

                                                                                            gcACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGATGCCCATAATAACTTCCGGc                                                        <  4:701163/149‑1 (MQ=255)

                                                                                             cACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGATGCCCATAATAACTTCCGGCg                                                       >  3:300464/1‑149 (MQ=255)

                                                                                              aCTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCTGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGATGCCCATAATAACTTCCGGCGt                                                      >  2:765029/1‑149 (MQ=255)

                                                                                                    ttACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAAt                                                                                       <  4:423965/110‑1 (MQ=255)

                                                                                                    ttACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAAt                                                                                       >  3:423965/1‑110 (MQ=255)

                                                                                                    ttACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGATGCCCATAATAACTTCCGGCGTTTGCAc                                                >  1:511622/1‑149 (MQ=255)

                                                                                                    ttACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGATGCCCATAATAACTTCCGGCGTTTGCAc                                                <  2:388308/149‑1 (MQ=255)

                                                                                                    ttACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGATGCCCATAATAACTTCCGGCGTTTGCAc                                                >  3:792594/1‑149 (MQ=255)

                                                                                                      aCGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGATGCCCATAATAACTTCCGGCGTTTGCACCt                                              <  3:15832/149‑1 (MQ=255)

                                                                                                        gATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGCGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGATGCCCATAATAACTTCCGGCGTTTGCACCTGa                                            >  2:120218/1‑149 (MQ=255)

                                                                                                        gATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGATGCCCATAATAACTTCCGGCGTTTGCACCTGa                                            >  1:811479/1‑149 (MQ=255)

                                                                                                            aTCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGATGCCCATAATAACTTCCGGCGTTTGCACCTGATTgg                                        >  3:120383/1‑149 (MQ=255)

                                                                                                             tCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGATGCCCATAATAACTTCCGGCGTTTGCACCTGATTGGc                                       <  1:590051/149‑1 (MQ=255)

                                                                                                             tCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGATGCCCATAATAACTTCCGGCGTTTGCACCTGATTGGc                                       >  2:883621/1‑149 (MQ=255)

                                                                                                             tCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGATGCCCATAATAACTTCCGGCGTTTGCACCTGATTGGc                                       >  4:740722/1‑149 (MQ=255)

                                                                                                               aaTTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGATGCCCATAATAACTTCCGGCGTTTGCACCTGATTGGCGa                                     <  2:802608/149‑1 (MQ=255)

                                                                                                                aTTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGATGCCCATAATAACTTCCGGCGTTTGCACCTGATTGGCGat                                    <  1:461541/149‑1 (MQ=255)

                                                                                                                  ttCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGATGCCCATAATAACTTCCGGCGTTTGCACCTGATTGGCGatat                                  <  4:300464/149‑1 (MQ=255)

                                                                                                                       ccACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGATGCCCATAATAACTTCCGGCGTTTGCACCTGATTGGCGATATATCCg                             <  2:595695/149‑1 (MQ=255)

                                                                                                                       ccACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGATGCCCATAATAACTTCCGGCGTTTGCACCTGATTGGCGATATATCCg                             >  1:358059/1‑149 (MQ=255)

                                                                                                                        cACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGAtgt                                                                                 <  3:530026/96‑1 (MQ=255)

                                                                                                                        cACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGAtgt                                                                                 >  4:530026/1‑96 (MQ=255)

                                                                                                                        cACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGATGCCCATAATAACTTCCGGCGTTTGCACCTGATTGGCGATATATCCGc                            <  3:381551/149‑1 (MQ=255)

                                                                                                                        cACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGATGCCCATAATAACTTCCGGCGTTTGCACCTGATTGGCGATATATCCGc                            >  3:66725/1‑149 (MQ=255)

                                                                                                                        cACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGATGCCCATAATAACTTCCGGCGTTTGCACCTGATTGGCGATATATCCGc                            >  1:119263/1‑149 (MQ=255)

                                                                                                                        cACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGATGCCCATAATAACTTCCGGCGTTTGCACCTGATTGGCGATATATCCGc                            >  1:379182/1‑149 (MQ=255)

                                                                                                                        cACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGATGCCCATAATAACTTCCGGCGTTTGCACCTGATTGGCGATATATCCGc                            >  1:80727/1‑149 (MQ=255)

                                                                                                                          cGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGATGCCCATAATAACTTCCGGCGTTTGCACCTGATTGGCGATATATCCGCtt                          >  1:13132/1‑149 (MQ=255)

                                                                                                                                 tttGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGATGCCCATAATAACTTCCGGCGTTTGCACCTGATTGGCGATATATCCGCTTAACCCCa                   >  1:458203/1‑149 (MQ=255)

                                                                                                                                        tttttATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGATGCCCATAATAACTTCCGGCGTTTGCACCTGATTGGCGATATATCCGCTTAACCCCAAAATaaa            <  2:358059/149‑1 (MQ=255)

                                                                                                                                        tttttATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGATGCCCATAATAACTTCCGGCGTTTGCACCTGATTGGCGATATATCCGCTTAACCCCAAAATaaa            <  1:781007/149‑1 (MQ=255)

                                                                                                                                        tttttATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGATGCCCATAATAACTTCCGGCGTTTGCACCTGATTGGCGATATATCCGCTTAACCCCAAAATaaa            <  2:13132/149‑1 (MQ=255)

                                                                                                                                         ttttATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGATGCCCATAATAACTTCCGGCGTTTGCACCTGATTGGCGATATATCCGCTTAACCCCAAAATAAAg           <  4:532169/149‑1 (MQ=255)

                                                                                                                                         ttttATCCGCTCACGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGATGCCCATAATAACTTCCGGCGtt                                                     >  1:205391/1‑107 (MQ=255)

                                                                                                                                         ttttATCCGCTCACGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGATGCCCATAATAACTTCCGGCGtt                                                     <  2:205391/107‑1 (MQ=255)

                                                                                                                                            tATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGATGCCCATAATAACTTCCGGCGTTTGCACCTGATTGGCGATATATCCGCTTAACCCCAAAATAAAGGCa        <  2:511622/149‑1 (MQ=255)

                                                                                                                                            tATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGATGCCCATAATAACTTCCGGCGTTTGCACCTGATTGGCGATATATCCGCTTAACCCCAAAATAAAGGCa        <  2:876194/149‑1 (MQ=255)

                                                                                                                                              tCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGATGCCCATAATAACTTCCGGCGTTTGCACCTGATTGGCGATATATCCGCTTAACCCCAAAATAAAGGCAgg      <  4:120383/149‑1 (MQ=255)

                                                                                                                                              tCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGATGCCCATAATAACTTCCGGCGTTTGCACCTGATTGGCGATATATCCGCTTAACCCCAAAATAAAGGCAgg      >  1:758993/1‑149 (MQ=255)

                                                                                                                                                 gTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGATGCCCATAATAACTTCCGGCGTTTGCACCTGATTGGCGATATATCCGCTTAACCCCAAAATAAAGGCAGGAAt   >  2:515318/1‑149 (MQ=255)

                                                                                                                                                  tGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGATGCCCATAATAACTTCCGGCGTTTGCACCTGATTGGCGATATATCCGCTTAACCCCAAAATAAAGGCAGGAAtt  >  1:635119/1‑149 (MQ=255)

CCATGGCCACTATTTTTCTCATAGTTGCACCTTTGATGTTCTGATTATTTTTATTGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGATGCCCATAATAACTTCCGGCGTTTGCACCTGATTGGCGATATATCCGCTTAACCCCAAAATAAAGGCAGGAATT  >  NC_000913/1692830‑1693124

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`