BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,703,593	A→G	5.2%	I239T (ATC→ACC)	ydgJ ←	putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,703,593	0	A	G	5.2%	360.4 / 4.2	108	I239T (ATC→ACC)	ydgJ	putative oxidoreductase
Reads supporting (aligned to +/- strand): ref base A (58/43); new base G (3/3); total (62/46)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 115 total aligned reads displayed.

GACACGTTCCTCACCTTCCACGCGGGTAAGTACGCCATCACGCATATCGTAGCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGTTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGCCAGCATGGTACCGTGTAAAATGACTCGCCGCTGTGGATAGGACAAGATGGCGTGGAAATAATCGGTCGACTGCGCTCCGGGCCGTAACTGTGCCAAATCTACCGTCATGCTGACCGGTAAAC  >  NC_000913/1703445‑1703740

gACACGTTCCTCACCTTCCACGCGGGTAAGTACGCCATCACGCATATCGTAGCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGTTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGa                                                                                                                                                     <  2:890596/149‑1 (MQ=255)

 acacGTTCCTCACCTTCCACGCGGGTAAGTACGCCATCACGCATATCGTAGCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGTTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGat                                                                                                                                                    <  3:720178/149‑1 (MQ=255)

 acacGTTCCTCACCTTCCACGCGGGTAAGTACGCCATCACGCATATCGTAGCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGTTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGat                                                                                                                                                    >  4:790271/1‑149 (MQ=255)

 acacGTTCCTCACCTTCCACGCGGGTAAGTACGCCATCACGCATATCGTAGCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGTTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGat                                                                                                                                                    <  4:887905/149‑1 (MQ=255)

  cacGTTCCTCACCTTCCACGCGGGTAAGTACGCCATCACGCATATCGTAGCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGTTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGata                                                                                                                                                   <  1:200658/149‑1 (MQ=255)

   acGTTCCTCACCTTCCACGCGGGTAAGTACGCCATCACGCATATCGTAGCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGTTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGatat                                                                                                                                                  >  3:546528/1‑149 (MQ=255)

         cTCACCTTCCACGCGGGTAAGTACGCCATCACGCATATCGTAGCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGTTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTg                                                                                                                                            >  2:430702/1‑149 (MQ=255)

          tCACCTTCCACGCGGGTAAGTACGCCATCACGCATATCGTAGCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGTTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGc                                                                                                                                           >  3:728339/1‑149 (MQ=255)

          tCACCTTCCACGCGGGTAAGTACGCCATCACGCATATCGTAGCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGTTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGc                                                                                                                                           >  2:613688/1‑149 (MQ=255)

          tCACCTTCCACGCGGGTAAGTACGCCATCACGCATATCGTAGCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGTTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGc                                                                                                                                           >  2:581373/1‑149 (MQ=255)

          tCACCTTCCACGCGGGTAAGTACGCCATCACGCATATCGTAGCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGTTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGc                                                                                                                                           >  4:797235/1‑149 (MQ=255)

           cACCTTCCACGCGGGTAAGTACGCCATCACGCATATCGTAGCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGTTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCt                                                                                                                                          <  4:16741/149‑1 (MQ=255)

            aCCTTCCACGCGGGTAAGTACGCCATCACGCATATCGTAGCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGTTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGGTATATCGTGcat                                                                                                                                         <  1:853632/149‑3 (MQ=255)

             ccTTCCACGCGGGTAAGTACGCCATCACGCATATCGTAGCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGTTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGa                                                                                                                                        <  4:185444/149‑1 (MQ=255)

              cTTCCACGCGGGTAAGTACGCCATCACGCATATCGTAGCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGTTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGAc                                                                                                                                       >  2:659392/1‑149 (MQ=255)

              cTTCCACGCGGGTAAGTACGCCATCACGCATATCGTAGCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGTTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGAc                                                                                                                                       >  1:878290/1‑149 (MQ=255)

               ttCCACGCGGGTAAGTACGCCATCACGCATATCGTAGCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGTTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACt                                                                                                                                      >  1:186069/1‑149 (MQ=255)

                   aCGCGGGTAAGTACGCCATCACGCATATCGTAGCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGTTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGc                                                                                                                                  >  2:316718/1‑149 (MQ=255)

                         gTAAGTACGCCATCACGCATATCGTAGCCCCCGTCTTCCTGCGGTAGCCGCTCGCCATTTTTCAGCCGTTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCGCGATATACCGTGCTGACTCAGCGGCTGc                                                                                                                            <  4:160945/149‑1 (MQ=255)

                               aCGCCATCACGCATATCGTAGCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGTTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGCCAGCAt                                                                                                                      <  2:808705/149‑1 (MQ=255)

                               aCGCCATCACGCATATCGTAGCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGTTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATACATGCACGATATACCGTGCTGACTCAGCGGCTGCCAGCAt                                                                                                                      <  4:536409/149‑1 (MQ=255)

                                  ccATCACGCATATCGTAGCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGTTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGTTGCCAGCATGGt                                                                                                                   >  2:522816/1‑149 (MQ=255)

                                   cATCACGCATATCGTAGCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGTTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGCCAGCATGGTa                                                                                                                  >  1:173748/1‑149 (MQ=255)

                                   cATCACGCATATCGTAGCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGTTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGCCAGCATGGTa                                                                                                                  >  4:821075/1‑149 (MQ=255)

                                          cATATCGTAGCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGTTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGCCAGCATGGTACCGTGTa                                                                                                           >  4:718035/1‑149 (MQ=255)

                                             atCGTAGCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGTTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGCCa                                                                                                                          <  4:851083/131‑1 (MQ=255)

                                             atCGTAGCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGTTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGCCa                                                                                                                          >  3:851083/1‑131 (MQ=255)

                                              tCGTAGCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGTTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGCCAGCATGGTACCGTGTAAAAt                                                                                                       <  4:860891/149‑1 (MQ=255)

                                                gTAGCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGTTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGCCAGCATGGTACCGTGTAAAATGa                                                                                                     <  1:87132/149‑1 (MQ=255)

                                                   gCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGTTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGTTGCCAGCATGGTACCGTGTAAAATGACTc                                                                                                  <  1:522816/149‑1 (MQ=255)

                                                    ccccAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGTTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGCCAGCATGGTACCGTGTAAAATGACTcg                                                                                                 >  3:197269/1‑149 (MQ=255)

                                                     cccAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGTTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGCCAGCATGGTACCGTGTAAAATGACTcgc                                                                                                <  4:358161/149‑1 (MQ=255)

                                                      ccAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGTTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGCCAGCATGGTACCGTGTAAAATGACTcgcc                                                                                               >  3:849229/1‑149 (MQ=255)

                                                      ccAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGTTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGCCAGCATGGTACCGTGTAAAATGACTcgcc                                                                                               >  3:635715/1‑149 (MQ=255)

                                                      ccAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGTTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGCCAGCATGGTACCGTGTAAAATGACTcgcc                                                                                               >  4:266967/1‑149 (MQ=255)

                                                            ttCCTGCGGTAGACGCTCGCCATTTTTCAGACGTTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGGTATATCGTGcat                                                                                                                                         <  3:81871/101‑3 (MQ=255)

                                                            ttCCTGCGGTAGACGCTCGCCATTTTTCAGACGTTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGGTATATCGTGcat                                                                                                                                         >  4:81871/1‑99 (MQ=255)

                                                            ttCCTGCGGTAGACGCTCGCCATTTTTCAGACGTTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGCCAGCATGGTACCGTGTAAAATGACTCGCCGCtgtg                                                                                         >  1:683659/1‑149 (MQ=255)

                                                            ttCCTGCGGTAGACGCTCGCCATTTTTCAGACGTTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGCCAGCATGGTACCGTGTAAAATGACTCGCCGCtgtg                                                                                         >  2:700217/1‑149 (MQ=255)

                                                              ccTGCGGTAGACGCTCGCCATTTTTCAGACGTTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGCCAGCATGGTACCGTGTAAAATGACTCGCCGCTGTGGa                                                                                       <  1:936725/149‑1 (MQ=255)

                                                               cTGCGGTAGACGCTCGCCATTTTTCAGACGTTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGCCAGCATGGTACCGTGTAAAATGACTCGCCGCTGTGGAt                                                                                      <  3:367460/149‑1 (MQ=255)

                                                                 gCGGTAGACGCTCGCCATTTTTCAGACGTTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGCCAGCATGGTACCGTGTAAAATGACTCGCCGCTGTGGATAg                                                                                    <  1:333693/149‑1 (MQ=255)

                                                                          gCTCGCCATTTTTCAGTCGTTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGCCAGCATGGTACCGTGTAAAATGACTCGCCGCTGTGGATAGGACAAGATg                                                                           <  2:533621/149‑1 (MQ=255)

                                                                            tCGCCATTTTTCAGACGTTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGCCAGCATGGTACCgt                                                                                                              >  3:827118/1‑112 (MQ=255)

                                                                              gCCATTTTTCAGACGTTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGCCAGCATGGTACCGTGTAAAATGACTCGCCGCTGTGGATAGGACAAGATGGCGt                                                                       >  3:174001/1‑149 (MQ=255)

                                                                              gCCATTTTTCAGACGTTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGCCAGCATGGTACCGTGTAAAATGACTCGCCGCTGTGGATAGGACAAGATGGCGt                                                                       >  2:257066/1‑149 (MQ=255)

                                                                               ccATTTTTCAGACGTTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGCCAGCATGGTACCGTGTAAAATGACTCGCCGCTGTGGATAGGACAAGATGGCGTg                                                                      >  4:704186/1‑149 (MQ=255)

                                                                               ccATTTTTCAGACGTTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGCCAGCATGGTACCGTGTAAAATGACTCGCCGCTGTGGATAGGACAAGATGGCGTg                                                                      <  4:902318/149‑1 (MQ=255)

                                                                                 aTTTTTCAGACGTTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGCCAGCATGGTACCGTGTAAAATGACTCGCCGCTGTGGATAGGACAAGATGGCGTGGa                                                                    <  3:704186/149‑1 (MQ=255)

                                                                                   ttttCAGACGTTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGCCAGCATGGTACCGTGTAAAATGACTCGCCGCTGTGGATAGGACAAGATGGCGTGGaaa                                                                  <  3:821075/149‑1 (MQ=255)

                                                                                   ttttCAGACGTTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGCCAGCATGGTACCGTGTAAAATGACTCGCCGCTGTGGATAGGACAAGATGGCGTGGaaa                                                                  <  1:700217/149‑1 (MQ=255)

                                                                                      tCAGACGTTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGCCAGCATGGTACCGTGTAAAATGACTCGCCGCTGTGGATAGGACAAGATGGCGTGGAaataa                                                               <  3:728601/149‑1 (MQ=255)

                                                                                      tCAGACGTTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGCCAGCATGGTACCGTGTAAAATGACTCGCCGCTGTGGATAGGACAAGATGGCGTGGAaataa                                                               >  3:128835/1‑149 (MQ=255)

                                                                                        aGACGTTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGCCAGCATGGTACCGTGTAAAATGACTCGCCGCTGTGGATAGGACAAGATGGCGTGGAAATAATc                                                             <  1:581373/149‑1 (MQ=255)

                                                                                          aCGTTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGCCAGCATGGTACCGTGTAAAATGACTCGTCGCTGTGGATAGGACAAGATGGCGTGGAAATAATCgg                                                           <  2:186069/149‑1 (MQ=255)

                                                                                           cGTTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGCCAGCATGGTACCGTGTAAAATGACTCGCCGCTGTGGATAGGACAAGATGGCGTGGAAATAATCGGt                                                          <  2:29313/149‑1 (MQ=255)

                                                                                            gTTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCGCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGCCAGCATGGTACCGTGGAAAATGACTCGCCGCGGTGGATAGGACAAGATCGCGTGGAAATAAGCGGTc                                                         >  2:503639/1‑149 (MQ=255)

                                                                                                ttcCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGCCAGCATGGTACCGTGTAAAATGACTCGCCGCTGTGGATAGGACAAGATGGCGTGGAAATAATCGGTCGACt                                                     >  4:363256/1‑149 (MQ=255)

                                                                                                   cTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGCCAGCATGGTACCGTGTAAAATGACTCGCCGCTGTGGATAGGACAAGATGGCGTGGAAATAATCGGTCGACTgcg                                                  >  1:535210/1‑149 (MQ=255)

                                                                                                     gtgGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGAGGCTGCCAGCATGGTACCGTGTAAAATGACTCGCCGCTGTGGATAGGACAAGATGGCGTGGAAATAATCGGTCGACTGCGCt                                                >  2:77388/1‑149 (MQ=255)

                                                                                                          tCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGCCAGCATGGTACCGTGTAAAATGACTCGCCGCTGTGGATAGGACAAGATGGCGTGGAAATAATCGGTCGACTGCGCTCCggg                                           >  4:596443/1‑149 (MQ=255)

                                                                                                          tCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGCCAGCATGGTACCGTGTAAAATGACTCGCCGCTGTGGATAGGACAAGATGGCGTGGAAATAATCGGTCGACTGCGCTCCggg                                           >  1:235865/1‑149 (MQ=255)

                                                                                                           cGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGCCAGCATGGTACCGTGTAAAATGACTCGCCGCTGTGGATAGGACAAGATGGCGTGGAAATAATCGGTCGACTGCGCTCCGGGc                                          >  2:753180/1‑149 (MQ=255)

                                                                                                            gAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGCCAGCATGGTACCGTGTAAAATGACTCGCCGCTGTGGATAGGACAAGATGGCGTGGAAATAATCGGTCGACTGCGCTCCGGGcc                                         >  4:723810/1‑149 (MQ=255)

                                                                                                               gCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGCCAGCATGGTACCGTGTAAAATGACTCGCCGCTGTGGATAGGACAAGATGGCGTGGAAATAATCGGTCGAc                                                      >  3:53677/1‑133 (MQ=255)

                                                                                                               gCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGCCAGCATGGTACCGTGTAAAATGACTCGCCGCTGTGGATAGGACAAGATGGCGTGGAAATAATCGGTCGAc                                                      <  4:53677/133‑1 (MQ=255)

                                                                                                               gCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGCCAGCATGGTACCGTGTAAAATGACTCGCCGCTGTGGATAGGACAAGATGGCGTGGAAATAATCGGTCGACTGCGCTCCGGGCCGTa                                      >  1:111804/1‑149 (MQ=255)

                                                                                                                ccATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGCCAGCATGGTACCGTGTAAAATGACTCGCCGCTGTGGATAGGACAAGATGGCGTGGAAATAATCGGTCGACTGCGCTCCGGGCCGTaa                                     <  2:797275/149‑1 (MQ=255)

                                                                                                                ccATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGCCAGCATGGTACCGTGTAAAATGACTCGCCGCTGTGGATAGGACAAGATGGCGTGGAAATAATCGGTCGACTGCGCTCCGGGCCGTaa                                     <  1:363880/149‑1 (MQ=255)

                                                                                                                 cATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGCCAGCATGGTACCGTGTAAAATGACTCGCCGCTGTGGATAGGACAAGATGGCGTGGAAATAATCGGTc                                                         >  2:433342/1‑128 (MQ=255)

                                                                                                                 cATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGCCAGCATGGTACCGTGTAAAATGACTCGCCGCTGTGGATAGGACAAGATGGCGTGGAAATAATCGGTc                                                         <  1:433342/128‑1 (MQ=255)

                                                                                                                 cATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGCCAGCATGGTACCGTGTAAAATGACTCGCCGCTGTGGATAGGACAAGATGGCGTGGAAATAATCGGTCGACTGCGCTCCGGGCCGTAAc                                    >  2:391707/1‑149 (MQ=255)

                                                                                                                     tttCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGCCAGCATGGTACCGTGTAAAATGACTCGCCGCTGTGGATAGGACAAGATGGCGTGGAAATAATCGGTCGACTGCGCTCCGGGCCGTAACtgtg                                >  4:379572/1‑149 (MQ=255)

                                                                                                                     tttCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGCCAGCATGGTACCGTGTAAAATGACTCGCCGCTGTGGATAGGACAAGATGGCGTGGAAATAATCGGTCGACTGCGCTCCGGGCCGTAACtgtg                                >  1:179308/1‑149 (MQ=255)

                                                                                                                     tttCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGCCAGCATGGTACCGTGTAAAATGACTCGCCGCTGTGGATAGGACAAGATGGCGTGGAAATAATCGGTCGACTGCGCTCCGGGCCGTAACtgtg                                >  3:467193/1‑149 (MQ=255)

                                                                                                                      ttCACATAACTGCCTCGGGATCCATGCTCGGTATACCGTGCTGACTCAGCGGCTGCCAGCAGGGGACCGTGTAAAATGACTCGCCGCTGTGGATAGGGCAAGATGGCGTGGAAATAAGCGGTCGACTGCGCTCCGGGCCGTAACTGTGc                               <  1:613688/149‑1 (MQ=255)

                                                                                                                      ttCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGCCAGCATGGTACCGTGTAAAATGACTCGCCGCTGTGGATAGGACAAGATGGCGTGGAAATAATCGGCCGACTGCGCTCCGGGCCGTAACTGTGc                               >  4:903961/1‑149 (MQ=255)

                                                                                                                         acaTAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGCCAGCATGGTACCGTGTAAAATGACTCGCCGCTGTGGATAGGACAAGATGGCGTGGAAATAATCGGTCGACTGCGCTCCGGGCCGTAACTGTGCCaa                            >  4:240333/1‑149 (MQ=255)

                                                                                                                          caTAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGCCAGCATGGTACCGTGTAAAATGACTCGCCGCTGTGGATAGGACAAGATGGCGTGGAAATAATCGGTCGACTGCGCTCCGGGCCGTAACTGTGCCaaa                           <  2:334983/149‑1 (MQ=255)

                                                                                                                            tAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGCCAGCATGGTACCGTGTAAAATGACTCGCCGCTGTGGATAGGACAAGATGGCGTGGAAATAATCGGTCGACTGCGCTCCGGGCCGTAACTGTGCCAAATc                         <  1:831318/149‑1 (MQ=255)

                                                                                                                             aaCTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGCCAGCATGGTACCGTGTAAAATGACTCGCCGCTGTGGATAGGACAAGATGGCGTGGAAATAATCGGTCGACTGCGCTCCGGGCCGTAACTGTGCCAAATCt                        >  1:676005/1‑149 (MQ=255)

                                                                                                                               cTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGCCAGCATGGTACCGTGTAAAATGACTCGCCGCTGTGGATAGGACAAGATGGCGTGGAAATAATCGGTCGACTGCGCTCCGGTCCGTAACTGTGCCAAATCTAc                      <  1:270113/149‑1 (MQ=255)

                                                                                                                               cTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGCCAGCATGGTACCGTGTAAAATGACTCGCCGCTGTGGATAGGACAAGATGGCGTGGAAATAATCGGTCGACTGCGCTCCGGGCCGTAACTGTGCCAAATCTAc                      <  3:266967/149‑1 (MQ=255)

                                                                                                                                 gCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGCCAGCATGGTACCGTGTAAAATGACTCGCCGCTGTGGATAGGACAAGATGGCGTGGAAATAATCGGTCGACTGCGCTCCGGGCCGTAACTGTGCCAAATCTACCg                    <  1:503045/149‑1 (MQ=255)

                                                                                                                                   cTCGGGATCCATGCACGCTATACCGTGCTGACTCAGCGGCTGCCAGCATGGTACCGTGTAAAATGACTCGCCGCTGTGGATAGGACAAGATCGCGTGGAAATAATCGGTCGACTGCGCTCCGGGCCGTAACTGTGCCAAATCTACCGTc                  >  4:177691/1‑149 (MQ=255)

                                                                                                                                   cTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGCCAGCATGGTACCGTGTAAAATGACTCGCCGCTGTGGATAGGACAAGATGGCGTGGAAATAATCGGTCGACTGCGCTCCGGGCCGTAACTGTGCCAAATCTACCGTc                  >  3:783845/1‑149 (MQ=255)

                                                                                                                                    tCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGCCAGCATGGTACCGTGTAAAATGACTCGCCGCTGTGGATAGGACAAGATGGCGTGGAAATAATCGGCCGACTGCGCTCCGGGCCGTAACTGTGCCAAATCTACCGTCa                 <  3:903961/149‑1 (MQ=255)

                                                                                                                                      gggATCCATGCACGATATACCGTGCTGACTCAGCGGCTGCCAGCATGGTACCGTGTAAAATGACTCGCCGCTGTGGATAGGACAAGATGGCGTGGAAATAATCGGTCGACTGCGCTCCGGGCCGTAACTGTGCCAAATCTACCGTCATg               >  3:529153/1‑149 (MQ=255)

                                                                                                                                       ggATCCATGCACGATATACCGTGCTGACTCAGCGGCTGCCAGCATGGTACCGTGTAAAATGACTCGCCGCTGTGGATAGGACAAGATGGCGTGGAAATAATCGGTCGACTGCGCTCCGGGCCGTAACTGTGCCAAATCTACCGTCATGc              <  1:430702/149‑1 (MQ=255)

                                                                                                                                       ggATCCATGCACGATATACCGTGCTGACTCAGCGGCTGCCAGCATGGTACCGTGTAAAATGACTCGCCGCTGTGGATAGGACAAGATGGCGTGGAAATAATCGGTCGACTGCGCTCCGGGCCGTAACTGTGCCAAATCTACCGTCATGc              <  3:775538/149‑1 (MQ=255)

                                                                                                                                        gATCCATGCACGATATACCGTGCTGACTCAGCGGCTGCCAGCATGGTACCGTGTAAAATGACTCGCCGCTGTGGATAGGACAAGATGGCGTGGAAATAATCGGTCGACTGCGCTCCGGGCCGTAACTGTGCCAAATCTACCGTCATGCt             >  2:274182/1‑149 (MQ=255)

                                                                                                                                               gCACGGTATATCGTGCTGACTCAGCGGCTGCCAGCATGGTACCGTGTAAAATGACTCGCCGCTGTGGATAGGACAAGATGGCGTGGAAATAATCGGTCGACTGCGCTCCGGGCCGTAACTGTGCCAAATCTACCGTCATGCTGACCGGt      >  4:120005/1‑149 (MQ=255)

                                                                                                                                               gCACGATATACCGTGCTGACTCAGCGGCTGCCAGCATGGTACCGTGTAAAATGACTCGCCGCTGTGGATAGGACAAGATGGCGTGGAAATAATCGGTCGACTGCGCTCCGGGCCGTAACTGTGCCAAATCTACCGTCATGCTGACCGGt      <  3:612809/149‑1 (MQ=255)

                                                                                                                                                cACGATATACCGTGCTGACTCAGCGGCTGCCAGCATGGTACCGTGTAAAATGACTCGCCGCTGTGGATAGGACAAGATGGCGTGGAAATAATCGGTCGACTGCGCTCCGGGCCGTAACTGTGCCAAATCTACCGTCATGCTGACCGGTa     >  1:145902/1‑149 (MQ=255)

                                                                                                                                                cACGATATACCGTGCTGACTCAGCGGCTGCCAGCATGGTACCGTGTAAAATGACTCGCCGCTGTGGATAGGACAAGATGGCGTGGAAATAATCGGTCGACTGCGCTCCGGGCCGTAACTGTGCCAAATCTACCGTCATGCTGACCGGTa     >  2:650126/1‑149 (MQ=255)

                                                                                                                                                 aCGGTATACCGTGCTGACTCAGCGGCTGCCAGCATGGTACCGTGTAAAATGACTCGCCGCTGTGGATAGGACAAGATGGCGTGGAAATAATCGGTCGACTGCGCTCCGGGCCGTAACTGTGCCAAATCTACCGTCATGCTGACCTGTaa    >  3:689479/1‑149 (MQ=255)

                                                                                                                                                 aCGATATACCGTGCTGACTCAGCGGCTGCCAGCATGGTACCGTGTAAAATGACTCGCCGCTGTGGATAGGACAAGATGGCGTGGAAATAATCGGTCGACTGCGCTCCGGGCCGTAACTGTGCCAAATCTACCGTCATGCTGACCGGTaa    >  2:572588/1‑149 (MQ=255)

                                                                                                                                                 aCGATATACCGTGCTGACTCAGCGGCTGCCAGCATGGTACCGTGTAAAATGACTCGCCGCTGTGGATAGGACAAGATGGCGTGGAAATAATCGGTCGACTGCGCTCCGGGCCGTAACTGTGCCAAATCTACCGTCATCCTGACGGTTaa    >  3:383216/1‑149 (MQ=255)

                                                                                                                                                   gATATACCGTGCTGACTCAGCGGCTGCCAGCATGGTACCGTGTAAAATGACTCGCCGCTGTGGATAGGACAAGATGGCGTGGAAATAATCGGTCGACTGCGCTCCGGGCCg                                        <  4:419373/111‑1 (MQ=255)

                                                                                                                                                   gATATACCGTGCTGACTCAGCGGCTGCCAGCATGGTACCGTGTAAAATGACTCGCCGCTGTGGATAGGACAAGATGGCGTGGAAATAATCGGTCGACTGCGCTCCGGGCCGTAACTGTGCCAAATCTACCGTCATGCTGACCGGTAAAc  <  4:849229/149‑1 (MQ=255)

GACACGTTCCTCACCTTCCACGCGGGTAAGTACGCCATCACGCATATCGTAGCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGTTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGCCAGCATGGTACCGTGTAAAATGACTCGCCGCTGTGGATAGGACAAGATGGCGTGGAAATAATCGGTCGACTGCGCTCCGGGCCGTAACTGTGCCAAATCTACCGTCATGCTGACCGGTAAAC  >  NC_000913/1703445‑1703740

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`