Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,808,680 T→A 9.9% intergenic (+24/+17) yniA → / ← yniB fructosamine kinase family protein/putative inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,808,6800TA 9.9% 384.4 / 26.6 121intergenic (+24/+17)yniA/yniBfructosamine kinase family protein/putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (50/59);  new base A (5/7);  total (55/66)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.23e-01

Only 100 of 122 total aligned reads displayed.

TGCCGATTTCCTTGAACGTCAACCGGTTTACCAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACG  >  NC_000913/1808533‑1808828
                                                                                                                                                   |                                                                                                                                                    
tGCCGATTTCCTTGAACGTCAACCGGTTTACCAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTc                                                                                                                                                     >  3:251632/1‑149 (MQ=255)
    gATTTCCTTGAACGTCAACCGGTTTACCAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTg                                                                                                                                                 >  1:64865/1‑149 (MQ=255)
         ccTTGAACGTCAACCGGTTTACCAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGGCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCACGGAGCGGcc                                                                                                                                            <  4:721799/149‑1 (MQ=255)
          cTTGAACGTCAACCGGTTTACCAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCACGGAGCGGcca                                                                                                                                           <  2:159326/149‑2 (MQ=255)
           ttGAACGTCAACCGGTTTACCAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCACGGAGCGGccat                                                                                                                                          >  2:274873/1‑147 (MQ=255)
            tGAACGTCAACCGGTTTACCAACTCTACACGCTTTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATGGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCACGGAGCGGCCatt                                                                                                                                         <  3:382336/149‑1 (MQ=255)
            tGAACGTCAACCGGTTTACCAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCttt                                                                                                                                         <  3:351511/149‑1 (MQ=255)
             gagcGTCAACCGGTTTACCAACTCTACACGCTGTTAAATCGTGCAACGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCACGGAGCGGCCatta                                                                                                                                        <  1:274873/146‑2 (MQ=255)
             gAACGTCAACCGGTTTACCAACTCTACACGCTGTTAAATCGTGCATGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCACGGAGCGGCCatta                                                                                                                                        <  1:506388/149‑2 (MQ=255)
             gAACGTCAACCGGTTTACCAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCtttt                                                                                                                                        <  1:364731/149‑1 (MQ=255)
             gAACGTCAACCGGTTTACCAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCATGCGGCCtttt                                                                                                                                        <  4:251632/149‑1 (MQ=255)
               aCGTCAACCGGTTTACCAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGa                                                                                                                                      <  4:194342/149‑1 (MQ=255)
               aCGTCAACCGGTTTACCAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGa                                                                                                                                      <  4:259724/149‑1 (MQ=255)
               aCGTCAACCGGTTTACCAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCACGGAGCGGccattatc                                                                                                                                      <  2:579052/149‑7 (MQ=255)
                cGTCAACCGGTTTACCAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGAt                                                                                                                                     <  1:661350/149‑1 (MQ=255)
                    aaCCGGTTTACCAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATtaaa                                                                                                                                 >  2:406677/1‑149 (MQ=255)
                    aaCCGGTTTACCAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATtaaa                                                                                                                                 >  3:500247/1‑149 (MQ=255)
                      ccGGTTTACCAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATtaaata                                                                                                                               >  2:256448/1‑149 (MQ=255)
                      ccGGTTTACCAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATtaaata                                                                                                                               >  3:494982/1‑149 (MQ=255)
                       cGGTTTACCAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATtaaataa                                                                                                                              >  2:30194/1‑149 (MQ=255)
                       cGGTTTACCAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATtaaataa                                                                                                                              >  3:239266/1‑149 (MQ=255)
                         gTTTACCAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCTGTGCGGCCTTTTGATTAAATAAAc                                                                                                                            <  3:474484/149‑1 (MQ=255)
                         gTTTACCAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAAc                                                                                                                            <  4:658313/149‑1 (MQ=255)
                         gTTTACCAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAAc                                                                                                                            >  4:118546/1‑149 (MQ=255)
                              ccAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAg                                                                                                                       >  3:506027/1‑149 (MQ=255)
                                    ctACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAgaga                                                                                                                 <  4:494982/149‑1 (MQ=255)
                                     tACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGaa                                                                                                                >  2:596883/1‑149 (MQ=255)
                                     tACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGaa                                                                                                                >  3:623326/1‑149 (MQ=255)
                                     tACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGaa                                                                                                                >  1:187595/1‑149 (MQ=255)
                                     tACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGaa                                                                                                                >  1:387629/1‑149 (MQ=255)
                                     tACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGaa                                                                                                                <  1:412608/149‑1 (MQ=255)
                                       cacGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGaaaa                                                                                                              <  4:159158/149‑1 (MQ=255)
                                       cacGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGaaaa                                                                                                              <  3:369636/149‑1 (MQ=255)
                                         cGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGa                                                                                                            >  4:432617/1‑149 (MQ=255)
                                          gCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGATAAGaa                                                                                                           <  1:218023/149‑1 (MQ=255)
                                          gCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGaa                                                                                                           <  3:830753/149‑1 (MQ=255)
                                          gCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGaa                                                                                                           <  3:463247/149‑1 (MQ=255)
                                            tGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAaca                                                                                                         <  4:500247/149‑1 (MQ=255)
                                            tGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAaca                                                                                                         <  1:249035/149‑1 (MQ=255)
                                            tGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATAAGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAaca                                                                                                         <  4:147955/149‑1 (MQ=255)
                                             gTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCGTGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAacac                                                                                                        <  2:86165/149‑1 (MQ=255)
                                              ttAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAAcaca                                                                                                       >  2:17284/1‑149 (MQ=255)
                                              ttAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAAcaca                                                                                                       >  3:88771/1‑149 (MQ=255)
                                              ttAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAAcaca                                                                                                       >  2:322544/1‑149 (MQ=255)
                                              ttAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGAAGAAATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGTTAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAAcaca                                                                                                       >  2:124093/1‑149 (MQ=255)
                                                aaaTCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATa                                                                                                     >  4:310283/1‑149 (MQ=255)
                                                 aaTCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATta                                                                                                                                   <  4:769654/118‑1 (MQ=255)
                                                       gCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTgca                                                                                              <  3:4129/149‑1 (MQ=255)
                                                           ggTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCaa                                                                                          >  1:620289/1‑149 (MQ=255)
                                                            gTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAAt                                                                                         >  3:172530/1‑149 (MQ=255)
                                                               aTTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACa                                                                                      <  4:861614/149‑1 (MQ=255)
                                                                 ttGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACaaa                                                                                    <  2:93877/149‑1 (MQ=255)
                                                                       gTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCg                                                                              >  1:840662/1‑149 (MQ=255)
                                                                          aGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCa                                                                           >  1:472964/1‑149 (MQ=255)
                                                                            cATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAgg                                                                         >  4:469752/1‑149 (MQ=255)
                                                                            cATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGCGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGaa                                                                                                           >  1:715758/1‑115 (MQ=255)
                                                                            cATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGCGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGaa                                                                                                           <  2:715758/115‑1 (MQ=255)
                                                                             aTTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGa                                                                        <  3:212243/149‑1 (MQ=255)
                                                                              tttGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGat                                                                       >  3:645959/1‑149 (MQ=255)
                                                                                   ttATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACa                                                                  >  4:86780/1‑149 (MQ=255)
                                                                                    tATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAg                                                                 <  1:354318/149‑1 (MQ=255)
                                                                                     aTTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGt                                                                <  2:840662/149‑1 (MQ=255)
                                                                                     aTTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGt                                                                <  4:244431/149‑1 (MQ=255)
                                                                                           cagcagTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGaaa                                                          >  3:353791/1‑149 (MQ=255)
                                                                                           cagcagTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGaaa                                                          <  2:226696/149‑1 (MQ=255)
                                                                                                 tCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGc                                                    >  2:891096/1‑149 (MQ=255)
                                                                                                 tCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGc                                                    <  2:664507/149‑1 (MQ=255)
                                                                                                 tCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGc                                                    >  3:902457/1‑149 (MQ=255)
                                                                                                  cATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCa                                                   >  4:61855/1‑149 (MQ=255)
                                                                                                  cATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCa                                                   <  3:118546/149‑1 (MQ=255)
                                                                                                  cATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCa                                                   <  2:472964/149‑1 (MQ=255)
                                                                                                  cATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCa                                                   <  2:22931/149‑1 (MQ=255)
                                                                                                    ttGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAgg                                                 >  4:37429/1‑149 (MQ=255)
                                                                                                          aGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATa                                           <  4:158990/149‑1 (MQ=255)
                                                                                                          aGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATa                                           <  2:387629/149‑1 (MQ=255)
                                                                                                              tattaGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTAt                                       >  4:504556/1‑149 (MQ=255)
                                                                                                                ttaGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATgg                                     >  2:542355/1‑149 (MQ=255)
                                                                                                                   gcagcaTGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGc                                  >  1:309164/1‑149 (MQ=255)
                                                                                                                      gcaTGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCaga                                                                                                                   <  1:886634/65‑1 (MQ=255)
                                                                                                                      gcaTGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGa                               >  2:413540/1‑149 (MQ=255)
                                                                                                                               gggTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATa                      <  2:590094/149‑1 (MQ=255)
                                                                                                                               gggTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATa                      <  4:645959/149‑1 (MQ=255)
                                                                                                                                ggTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATAc                     <  3:432617/149‑1 (MQ=255)
                                                                                                                                    gAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGa                 <  4:353791/149‑1 (MQ=255)
                                                                                                                                    gAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGa                 <  2:84131/149‑1 (MQ=255)
                                                                                                                                       gATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTc              >  2:400556/1‑149 (MQ=255)
                                                                                                                                       gATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTc              >  4:479710/1‑149 (MQ=255)
                                                                                                                                        caaaaggCCGCACGGAGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCa             >  2:188046/6‑149 (MQ=255)
                                                                                                                                         tAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCa                                                   >  3:95687/1‑110 (MQ=255)
                                                                                                                                          aaaggCCGCACGGAGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAAt           >  1:842727/4‑149 (MQ=255)
                                                                                                                                          aaaggCCGCACGGAGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAAt           >  3:221402/4‑149 (MQ=255)
                                                                                                                                          aaTGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAAt           <  4:506027/149‑1 (MQ=255)
                                                                                                                                           aaggCCGCACGGAGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAAtt          >  1:881477/3‑149 (MQ=255)
                                                                                                                                           aTGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAAtt          <  4:681051/149‑1 (MQ=255)
                                                                                                                                            tGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAAttt         <  1:480046/149‑1 (MQ=255)
                                                                                                                                            tGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAAttt         <  4:902457/149‑1 (MQ=255)
                                                                                                                                            tGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACCGGACTCAAttt         <  2:887829/149‑1 (MQ=255)
                                                                                                                                                cGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTc     <  3:281951/149‑1 (MQ=255)
                                                                                                                                                cGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTc     <  1:148758/149‑1 (MQ=255)
                                                                                                                                                   tCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCAcg  >  1:388543/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
TGCCGATTTCCTTGAACGTCAACCGGTTTACCAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACG  >  NC_000913/1808533‑1808828

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: