Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,863,803 G→A 5.7% intergenic (+37/‑47) gapA → / → yeaD glyceraldehyde‑3‑phosphate dehydrogenase A/D‑hexose‑6‑phosphate epimerase‑like protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,863,8030GA 5.7% 344.5 / 6.6 105intergenic (+37/‑47)gapA/yeaDglyceraldehyde‑3‑phosphate dehydrogenase A/D‑hexose‑6‑phosphate epimerase‑like protein
Reads supporting (aligned to +/- strand):  ref base G (54/45);  new base A (2/4);  total (56/49)
Fisher's exact test for biased strand distribution p-value = 4.14e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01

Only 100 of 108 total aligned reads displayed.

AAAGCTGGTATCGCTCTGAACGACAACTTCGTGAAACTGGTATCCTGGTACGACAACGAAACCGGTTACTCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCCGTCGTAAACTGGATGAACTGGACCTCATTGTGGTCGATCATCCC  >  NC_000913/1863656‑1863951
                                                                                                                                                   |                                                                                                                                                    
aaaGCTGGTATCGCTCTGAACGACAACTTCGTGAAACTGGTATCCTGGTACGACAACGAAACCGGTTACTCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCgg                                                                                                                                                     <  1:588906/149‑1 (MQ=255)
 aaGCTGGTATCGCTCTGAACGACAACTGCGTGAAACTGGTATCCTGGTACGACAACGAAACCGGTTACTCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGt                                                                                                                                                    <  2:533315/149‑1 (MQ=255)
       gTATCGCTCTGAACGACAACTTCGTGAAACTGGTATCCTGGTACGACAACGAAACCGGTTACTCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGctct                                                                                                                                              >  1:348690/1‑149 (MQ=255)
        tATCGCTCTGAACGACAACTTCGTGAAACTGGTATCCTGGTACGACAACGAAACCGGTTACTCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCtt                                                                                                                                             >  3:498210/1‑149 (MQ=255)
         aTCGCTCTGAACGACAACTTCGTGAAACTGGTATCCTGGTACGACAACGAAACCGGTTACTCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCttt                                                                                                                                            <  3:386391/149‑1 (MQ=255)
         aTCGCTCTGAACGACAACTTCGTGAAACTGGTATCCTGGTACGACAACGAAACCGGTTACTCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACCGAAGTCGCTCttt                                                                                                                                            <  3:128919/149‑1 (MQ=255)
          tCGCTCTGAACGACAACTTCGTGAAACTGGTATCCTGGTACGACAACGAAACCGGTTACTCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACCGAAGTCGCTCtttt                                                                                                                                           <  3:236088/149‑1 (MQ=255)
                     gACAACTTCGTGAAACTGGTATCCTGGTACGACAACGAAACCGGTTACTCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGAt                                                                                                                                >  2:609514/1‑149 (MQ=255)
                        aaCTTCGTGAAACTGGTATCCTGGTACGACAACGAAACCGGTTACTCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATaaa                                                                                                                             >  2:690688/1‑149 (MQ=255)
                          cTTCGTGAAACTGGTATCCTGGTACGACAACGAAACCGGTTACTCCAACAAAGTTCTTGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACCGAAGTCGCTCttt                                                                                                                                            <  2:435345/132‑1 (MQ=255)
                          cTTCGTGAAACTGGTATCCTGGTACGACAACGAAACCGGTTACTCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAAt                                                                                                                           <  3:735066/149‑1 (MQ=255)
                          cTTCGTGAAACTGGTATCCTGGTACGACAACGAAACCGGTTACTCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACCGAAGTCGCTCttt                                                                                                                                            >  1:435345/1‑132 (MQ=255)
                           ttCGTGAAACTGGTATCCTGGTACGACAACGAAACCGGTTACTCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACCGAAGTCGCTCttt                                                                                                                                            <  3:465131/131‑1 (MQ=255)
                           ttCGTGAAACTGGTATCCTGGTACGACAACGAAACCGGTTACTCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACCGAAGTCGCTCttt                                                                                                                                            >  4:465131/1‑131 (MQ=255)
                             cGTGAAACTGGTATCCTGGTACGACAACGAAACCGGTTACTCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGaa                                                                                                                        <  1:91923/149‑1 (MQ=255)
                             cGTGAAACTGGTATCCTGGTACGACAACGAAACCGGTTACTCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGaa                                                                                                                        <  4:44400/149‑1 (MQ=255)
                              gTGAAACTGGTATCCTGGTACGACAACGAAACCGGTTACTCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAg                                                                                                                       <  1:486082/149‑1 (MQ=255)
                               tGAAACTGGTATCCTTGTACGACAACGAAACCGGTTACTCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGACGCTATTTTTTTTACCTGATAAAATGAAGt                                                                                                                      >  2:826151/1‑149 (MQ=255)
                                  aaCTGGTATCCTGGTACGACAACGAAACCGGTTACTCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTaa                                                                                                                   >  2:104869/1‑149 (MQ=255)
                                    cTGGTATCCTGGTACGACAACGAAACCGGTTACTCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATTAGGTTAAAg                                                                                                                 <  2:102996/149‑1 (MQ=255)
                                     tGGTATCCTGGTACGACAACGAAACCGGTTACTCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAgg                                                                                                                >  2:909534/1‑149 (MQ=255)
                                       gTATCCTGGTACGACAACGAAACCGGTTACTCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGAc                                                                                                              >  4:139523/1‑149 (MQ=255)
                                        tATCCTGGTACGACAACGAAACCGGTTACTCCAACAAAGTTCTGTACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACt                                                                                                             >  4:133324/1‑149 (MQ=255)
                                        tATCCTGGTACGACAACGAAACCGGTTACTCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACt                                                                                                             >  4:358572/1‑149 (MQ=255)
                                         aTCCTGGTACGACAACGAAACCGGTTACTCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTg                                                                                                            <  1:368254/149‑1 (MQ=255)
                                                tACGACAACGAAACCGGTTACTCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATg                                                                                                     >  2:541644/1‑149 (MQ=255)
                                                  cGACAACGAAACCGGTTACTCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGAt                                                                                                   <  3:701954/149‑1 (MQ=255)
                                                    aCAACGAAACCGGTTACTCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTa                                                                                                 >  3:379094/1‑149 (MQ=255)
                                                      aaCGAAACCGGTTACTCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAg                                                                                               >  2:808049/1‑149 (MQ=255)
                                                      aaCGAAACCGGTTACTCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAg                                                                                               <  2:810931/149‑1 (MQ=255)
                                                             ccGGTTACTCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAACAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAGTGAAGTTAAAGGACTGCGTCATGATTAAGAAAAttt                                                                                        >  4:625426/1‑149 (MQ=255)
                                                               ggTTACTCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAAttttt                                                                                      <  3:416602/149‑1 (MQ=255)
                                                               ggTTACTCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAAttttt                                                                                      <  1:226930/149‑1 (MQ=255)
                                                               ggTTACTCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAAttttt                                                                                      >  1:922616/1‑149 (MQ=255)
                                                                     tCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCtt                                                                                >  4:32336/1‑149 (MQ=255)
                                                                      ccAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTc                                                                               <  4:498210/149‑1 (MQ=255)
                                                                        aacaaAGTTCTGGACTTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTaa                                                                                                <  4:685126/130‑1 (MQ=255)
                                                                        aacaaAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTaa                                                                                                >  3:685126/1‑130 (MQ=255)
                                                                        aacaaAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGa                                                                                              >  1:666628/1‑132 (MQ=255)
                                                                        aacaaAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGa                                                                                              <  2:666628/132‑1 (MQ=255)
                                                                        aacaaAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAAttttt                                                                                      >  4:641683/1‑140 (MQ=255)
                                                                        aacaaAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAAttttt                                                                                      <  3:641683/140‑1 (MQ=255)
                                                                        aacaaAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCg                                                                             <  3:139523/149‑1 (MQ=255)
                                                                        aacaaAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCg                                                                             >  1:411439/1‑149 (MQ=255)
                                                                        aacaaAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCg                                                                             >  4:49711/1‑149 (MQ=255)
                                                                        aacaaAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCg                                                                             <  3:49711/149‑1 (MQ=255)
                                                                         acaaAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCgg                                                                            >  4:423960/1‑149 (MQ=255)
                                                                          caaAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGaa                                                                                                                        >  3:563657/1‑104 (MQ=255)
                                                                          caaAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGaa                                                                                                                        <  4:563657/104‑1 (MQ=255)
                                                                           aaaGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTc                                                                          <  1:609514/149‑1 (MQ=255)
                                                                           aaaGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTc                                                                          >  2:189603/1‑149 (MQ=255)
                                                                            aaGCTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCa                                                                         >  2:215826/1‑149 (MQ=255)
                                                                              gTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATc                                                                       <  2:411439/149‑1 (MQ=255)
                                                                                  tGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAAc                                                                   <  3:32336/149‑1 (MQ=255)
                                                                                   ggACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACa                                                                  >  3:282312/1‑149 (MQ=255)
                                                                                    gACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCttttttt                                                                                                                                        >  1:559990/1‑78 (MQ=255)
                                                                                    gACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCttttttt                                                                                                                                        <  2:559990/78‑1 (MQ=255)
                                                                                      ccTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAAt                                                               <  1:830007/149‑1 (MQ=255)
                                                                                        tGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGc                                                                                                           >  1:667272/1‑103 (MQ=255)
                                                                                        tGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGc                                                                                                           <  2:667272/103‑1 (MQ=255)
                                                                                           tCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTccc                                                          <  3:423960/149‑1 (MQ=255)
                                                                                             gCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCt                                                        >  2:833903/1‑149 (MQ=255)
                                                                                             gCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCt                                                        >  3:92620/1‑149 (MQ=255)
                                                                                               tCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGt                                                      >  2:706574/1‑149 (MQ=255)
                                                                                                cacaTCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTc                                                     <  1:139938/149‑1 (MQ=255)
                                                                                                cacaTCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTc                                                     <  2:489918/149‑1 (MQ=255)
                                                                                                 acaTCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTaaa                                                                                                                  >  1:490116/1‑87 (MQ=255)
                                                                                                 acaTCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTaaa                                                                                                                  <  2:490116/87‑1 (MQ=255)
                                                                                                     ctcCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCtctc                                                <  4:494355/149‑1 (MQ=255)
                                                                                                     ctcCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCtctc                                                >  2:492969/1‑149 (MQ=255)
                                                                                                      tcCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCGCTGTCCTCTcc                                               <  3:540397/149‑1 (MQ=255)
                                                                                                      tcCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCTCTcc                                               >  1:171863/1‑149 (MQ=255)
                                                                                                       ccAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCTCTccc                                              <  3:359293/149‑1 (MQ=255)
                                                                                                         aaaTAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCcgt                                            >  1:550494/1‑149 (MQ=255)
                                                                                                         aaaTAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCcgt                                            >  1:62503/1‑149 (MQ=255)
                                                                                                          aaTAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCcgtc                                           >  4:897861/1‑149 (MQ=255)
                                                                                                           aTAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCcgtcg                                          >  2:151969/1‑149 (MQ=255)
                                                                                                               gTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCCGTCGTaaa                                      <  1:690688/149‑1 (MQ=255)
                                                                                                                    gaTGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCCGTCGTAAACTGGa                                 <  2:171863/149‑1 (MQ=255)
                                                                                                                     aTGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCCGTCGTAAACTGGAt                                >  4:38236/1‑149 (MQ=255)
                                                                                                                         cacTGTGATCTAAAAAGAGCGACTTCGGTCGTTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCCGTCGTAAACTGGATGAAc                            <  1:826151/149‑1 (MQ=255)
                                                                                                                             gtgATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCCGTCGTAAACTGGATGAACTGGa                        <  3:897861/149‑1 (MQ=255)
                                                                                                                             gtgATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCCGTCGTAAACTGGATGAACTGGa                        <  3:917376/149‑1 (MQ=255)
                                                                                                                             gtgATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCCGTCGTAAACTGGATGAACTGGa                        <  1:104869/149‑1 (MQ=255)
                                                                                                                                aTCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCCGTCGTAAACTGGATGAACTGGACCt                     >  3:781037/1‑149 (MQ=255)
                                                                                                                                aTCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCCGTCGTAAACTGGATGAACTGGACCt                     >  1:553434/1‑149 (MQ=255)
                                                                                                                                   tAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCCGTCGTAAACTGGATGAACTGGACCTCAt                  >  1:176060/1‑149 (MQ=255)
                                                                                                                                   tAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCCGTCGTAAACTGGATGAACTGGACCTCAt                  >  3:583318/1‑149 (MQ=255)
                                                                                                                                      aaaGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCCGTCGTAAACTGGATGAACTGGACCTCATtgt               >  1:566407/1‑149 (MQ=255)
                                                                                                                                        agagCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCCGTCGTAAACTGGATGAACTGGACCTCa                   <  2:513860/143‑1 (MQ=255)
                                                                                                                                        agagCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCCGTCGTAAACTGGATGAACTGGACCTCa                   >  1:513860/1‑143 (MQ=255)
                                                                                                                                         gagCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATc                                                                       >  2:198692/1‑90 (MQ=255)
                                                                                                                                         gagCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATc                                                                       <  1:198692/90‑1 (MQ=255)
                                                                                                                                         gagCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCCGTCGTAAACTGGATGAACTGGACCTCATTGTGGt            <  2:309388/149‑1 (MQ=255)
                                                                                                                                            cGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACAGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCCGTCGTAAACTGGATGAACTGGACCTCATTGTGGTCGa         >  1:605833/1‑149 (MQ=255)
                                                                                                                                             gACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCCGTCGTAAACTGGATGAACTGGACCTCATTGTGGTCGat        <  1:747706/149‑1 (MQ=255)
                                                                                                                                                ttCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCCGTCGTAAACTGGATGAACTGGACCTCATTGTGGTCGatcat     >  1:653197/1‑149 (MQ=255)
                                                                                                                                                 tCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCCGTCGTAAACTGGATGAACTGGACCTCATTGTGGTCGatcatc    <  4:282312/149‑1 (MQ=255)
                                                                                                                                                   ggTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCCGTCGTAAACTGGATGAACTGGACCTCATTGTGGTCGATCATccc  <  2:518881/149‑1 (MQ=255)
                                                                                                                                                   ggTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCCGTCGTAAACTGGATGAACTGGACCTCATTGTGGTCGATCATccc  <  2:62503/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
AAAGCTGGTATCGCTCTGAACGACAACTTCGTGAAACTGGTATCCTGGTACGACAACGAAACCGGTTACTCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCCGTCGTAAACTGGATGAACTGGACCTCATTGTGGTCGATCATCCC  >  NC_000913/1863656‑1863951

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: