Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,888,032	T→A	5.4%	intergenic (‑41/+29)	rnd ← / ← fadD	ribonuclease D/acyl‑CoA synthetase (long‑chain‑fatty‑acid‑‑CoA ligase)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,888,032	0	T	A	5.4%	258.6 / 4.7	74	intergenic (‑41/+29)	rnd/fadD	ribonuclease D/acyl‑CoA synthetase (long‑chain‑fatty‑acid‑‑CoA ligase)
Reads supporting (aligned to +/- strand):  ref base T (41/29);  new base A (2/2);  total (43/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.70e-01

GTGCGCGTACGAACAAATTCAGTATCCAGGGCTATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCGGTAACTCATCACGAAACTCCACCAGCTTCGGTACTTTGTATC  >  NC_000913/1887885‑1888179                                                                                                                                                    |                                                                                                                                                    gTGCGCGTACGAACAAATTCAGTATCCAGGGCTATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTa                                                                                                                                                    <  3:700621/149‑1 (MQ=255)       gTACGAACAAATTCAGTATCCAGGGCTATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGc                                                                                                                                              <  2:737124/149‑1 (MQ=255)          cGAACAAATTCAGTATCCAGGGCTATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGTTAATCCGGCGtt                                                                                                                                           <  1:763627/149‑2 (MQ=255)          cGAACAAATTCAGTATCCAGGGCTATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTc                                                                                                                                           <  4:276719/149‑1 (MQ=255)           gAACAAATTCAGTATCCAGGGCTATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCt                                                                                                                                          >  3:200007/1‑149 (MQ=255)           gAACAAATTCAGTATCCAGGGCTATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCt                                                                                                                                          >  4:919795/1‑149 (MQ=255)           gAACAAATTCAGTATCCAGGGCTATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCt                                                                                                                                          >  3:738139/1‑149 (MQ=255)           gAACAAATTCAGTATCCAGGGCTATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCt                                                                                                                                          >  3:15870/1‑149 (MQ=255)           gAACAAATTCAGTATCCAGGGCTATCGCCGGAAAGGCACGGACGGATTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCt                                                                                                                                          >  1:878375/1‑149 (MQ=255)              cAAATTCAGTATCCAGGGCTATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTgac                                                                                                                                       >  2:912381/1‑149 (MQ=255)                aaTTCAGTATCCAGGGCTATCGCCGGAAAGGCACGGACGGCTTCACATAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTgacga                                                                                                                                     >  2:678041/1‑149 (MQ=255)                aaTTCAGTATCCAGGGCTATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTgacga                                                                                                                                     <  2:13774/149‑1 (MQ=255)                  ttCAGTATCCAGGGCTATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTGGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACgact                                                                                                                                   >  2:275114/1‑149 (MQ=255)                   tCAGTATCCAGGGCTATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACgactg                                                                                                                                  <  3:283554/149‑1 (MQ=255)                    cAGTATCCAGGGCTATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACgactga                                                                                                                                 <  3:116880/149‑1 (MQ=255)                      gTATCCAGGGCTATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACgactgact                                                                                                                               >  4:384255/1‑149 (MQ=255)                       tATCCAGGGCTATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACtt                                                                                                                              >  3:178666/1‑149 (MQ=255)                            aGGGCTATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGc                                                                                                                         >  4:546748/1‑149 (MQ=255)                            aGGGCTATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGc                                                                                                                         >  4:296265/1‑149 (MQ=255)                            aGGGCTATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGc                                                                                                                         >  2:890396/1‑149 (MQ=255)                               gCTATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCa                                                                                                                      <  4:15870/149‑1 (MQ=255)                                  aTCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGc                                                                                                                   >  4:759902/1‑149 (MQ=255)                                      ccGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTa                                                                                                               <  1:912381/149‑1 (MQ=255)                                        ggAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTAtt                                                                                                             <  1:275114/149‑1 (MQ=255)                                           aaGGCACGGACGGCTTCACACAAAGAAGCCGGCGCATCTTCCGTGGTAATCATTTGTGAATTCAAATTTTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTc                                                                                                          <  2:862214/149‑1 (MQ=255)                                           aaGGCACGGACGGCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTc                                                                                                          <  1:41568/149‑1 (MQ=255)                                            aGGCACGGACGGCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTcc                                                                                                         <  1:537197/149‑1 (MQ=255)                                             ggCACGGACGGCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCa                                                                                                        <  3:83379/149‑1 (MQ=255)                                              gCACGGACGGCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCAc                                                                                                       >  2:577713/1‑149 (MQ=255)                                              gCACGGACGGCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCAc                                                                                                       >  1:415677/1‑149 (MQ=255)                                                        cTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCcgcgc                                                                                             <  4:836895/149‑1 (MQ=255)                                                         ttCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCcgcgcg                                                                                            >  2:454590/1‑149 (MQ=255)                                                          tCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCgcgcgc                                                                                           >  4:266369/1‑149 (MQ=255)                                                            acacaAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACTGGCGCCTGACGACTGACTTAACGCTCAGGCTATATTGTCCACTTCGCCGCGCGCtt                                                                                         >  2:298600/1‑149 (MQ=255)                                                            acacaAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCtt                                                                                         >  1:698348/1‑149 (MQ=255)                                                            acacaAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCtt                                                                                         >  4:496852/1‑149 (MQ=255)                                                              acaAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCg                                                                                       >  1:258688/1‑149 (MQ=255)                                                              acaAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCg                                                                                       >  3:868326/1‑149 (MQ=255)                                                                aaaGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGTTAATCCGGCGttttt                                                                                                                                        >  3:202318/1‑94 (MQ=255)                                                                aaaGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGTTAATCCGGCGttttt                                                                                                                                        <  4:202318/98‑5 (MQ=255)                                                                  agaagCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCAc                                                                                   >  4:459295/1‑149 (MQ=255)                                                                  agaagCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCAc                                                                                   <  4:356960/149‑1 (MQ=255)                                                                   gaagCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACg                                                                                  >  2:113538/1‑149 (MQ=255)                                                                             cATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCtcgt                                                                        <  3:919795/149‑1 (MQ=255)                                                                              aTCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCtcgtc                                                                       <  2:664935/149‑1 (MQ=255)                                                                                      tGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAAtt                                                               >  3:502782/1‑149 (MQ=255)                                                                                         tAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAAttttt                                                            >  4:323538/1‑149 (MQ=255)                                                                                            tCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTc                                                                                        <  3:880715/118‑1 (MQ=255)                                                                                            tCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCAGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTc                                                                                        >  4:880715/1‑118 (MQ=255)                                                                                              aTTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGAc                                                       <  3:318612/149‑1 (MQ=255)                                                                                              aTTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGAc                                                       >  2:145885/1‑149 (MQ=255)                                                                                                  ggTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTa                                                   >  1:438126/1‑149 (MQ=255)                                                                                                  ggTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTa                                                   >  3:324331/1‑149 (MQ=255)                                                                                                  ggTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTa                                                   <  1:799051/149‑1 (MQ=255)                                                                                                    tAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGa                                                 <  1:525904/149‑1 (MQ=255)                                                                                                     aaTTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGAt                                                <  2:131679/149‑1 (MQ=255)                                                                                                              tGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCGGTAAc                                       <  3:521160/149‑1 (MQ=255)                                                                                                                    ctctTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCGGTAACtcatca                                 <  3:4946/149‑1 (MQ=255)                                                                                                                       tttAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGAc                                                       <  2:330755/124‑1 (MQ=255)                                                                                                                       tttAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGAc                                                       >  1:330755/1‑124 (MQ=255)                                                                                                                              ggCGTCAAAAAAACCGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCGGTAACTCATCACGAAACTcca                       <  4:502782/149‑1 (MQ=255)                                                                                                                                cGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCGGTAACTCATCACGAAACTccacc                     >  3:95156/1‑149 (MQ=255)                                                                                                                                 gTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCGGTAACTCATCACGAAACTccacca                    >  4:691343/1‑149 (MQ=255)                                                                                                                                    aaaaaaaaCGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCGGTAACTCATCACGAAACTCCACCAGCt                 >  1:633311/1‑149 (MQ=255)                                                                                                                                    aaaaaaaaCGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCGGTAACTCATCACGAAACTCCACCAGCt                 >  3:23711/1‑149 (MQ=255)                                                                                                                                    aaaaaaaaCGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCGGTAACTCATCACGAAACTCCACCAGCt                 >  3:716659/1‑149 (MQ=255)                                                                                                                                         agaCGCCGGTTAATCCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCGGTAACTCATCACGAAACTCCACCAGCTTCGGt            >  1:434504/3‑149 (MQ=255)                                                                                                                                           aCGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCGGTAACTCATCACGAAACTCCACCAGCTTCGGTAc          <  1:779747/149‑1 (MQ=255)                                                                                                                                             gCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGt                                                                                      <  4:684413/71‑1 (MQ=255)                                                                                                                                             gCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGt                                                                                      >  3:684413/1‑71 (MQ=255)                                                                                                                                             gCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCGGTAACTCATCACGAAACTccac                      >  2:336497/1‑135 (MQ=255)                                                                                                                                             gCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCGGTAACTCATCACGAAACTccac                      <  1:336497/135‑1 (MQ=255)                                                                                                                                             gCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCGGTAACTCATCACGAAACTCCACCAGCTTCGGTACtt        >  4:797723/1‑149 (MQ=255)                                                                                                                                               cGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCGGTAACTCATCACGAAACTCCACCAGCTTCGGTACTTTg      >  1:856649/1‑149 (MQ=255)                                                                                                                                                   ttAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTCAGTGTCCACTTTGCCGCGCGCTTCGGCACGTAATTCTCGTCGCAAAACGTTTCCGACGTTAGATTTCGGTAACTCATCACGAAACTCCACCAGCATCGGTACTTTGTATc  <  4:269839/149‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                    GTGCGCGTACGAACAAATTCAGTATCCAGGGCTATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCGGTAACTCATCACGAAACTCCACCAGCTTCGGTACTTTGTATC  >  NC_000913/1887885‑1888179

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑