| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,981,814 | T→A | 5.7% | T192S (ACG→TCG) | otsB ← | trehalose‑6‑phosphate phosphatase, biosynthetic |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,981,814 | 0 | T | A | 5.7% | 372.3 / 9.3 | 106 | T192S (ACG→TCG) | otsB | trehalose‑6‑phosphate phosphatase, biosynthetic |
| Reads supporting (aligned to +/- strand): ref base T (46/54); new base A (2/4); total (48/58) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.87e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.65e-01 | |||||||||||
Only 100 of 106 total aligned reads displayed.
AAGCCAGCTCCAGACATCCGGCACACCCGCCAGTCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAA‑CGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCGACAACACACTTTCCCTGCTGTAACGCCATTTGTGGCCAGATCTGAGTAATACGTTGCGCTAATGTCAT > NC_000913/1981668‑1981958 | aaGCCAGCTCCAGACATCCGGCACACCCGCCAGTCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAA‑CGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTc < 3:481164/149‑1 (MQ=255)aaGCCAGCTCCAGACATCCGGCACACCCGCCAGTCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAA‑CGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTc > 2:788496/1‑149 (MQ=255)aaGCCAGCTCCAGACATCCGGCACACCCGCCAGTCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAA‑CGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTc > 1:309754/1‑149 (MQ=255) gCCAGCTCCAGACATCCGGCACACCCGCCAGTCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAA‑CGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGc > 2:288497/1‑149 (MQ=255) cAGCTCCAGACATCCGGCACACCCGCCAGTCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAA‑CGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGccc < 4:475991/149‑1 (MQ=255) cAGCTCCAGACATCCGGCACACCCGCCAGTCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAA‑CGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGccc > 3:97738/1‑149 (MQ=255) gCTCCAGACATCCGGCACACCCGCCAGTCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAA‑CGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGa < 4:454500/149‑1 (MQ=255) cTCCAGACATCCGGCACACCCGCCAGTCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAA‑CGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGAt < 3:247306/149‑1 (MQ=255) cTCCAGACATCCGGCACACCCGCCAGTCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAA‑CGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGAACGCCCgta < 4:870016/149‑3 (MQ=255) tCCAGACATCCGGCACACCCGCCAGTCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAA‑CGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGAACGCCCgtaa < 2:793994/149‑4 (MQ=255) ccAGACATCCGGCACACCCGCCAGTCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAA‑CGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGAACGCCCgtatt < 3:793868/149‑5 (MQ=255) aGACATCCGGCACACCCGCCAGTCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAA‑CGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAg > 3:476964/1‑149 (MQ=255) aGACATCCGGCACACCCGCCAGTCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAA‑CGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAg > 1:309157/1‑149 (MQ=255) aGACATCCGGCACACCCGCCAGTCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAA‑CGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAg > 1:190100/1‑149 (MQ=255) aGACATCCGGCACACCCGCCAGTCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAA‑CGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAg < 1:936922/149‑1 (MQ=255) gACATCCGGCACACCCGCCAGTCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAA‑CGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGAACGCCCgtatttct < 4:773293/149‑8 (MQ=255) aCATCCGGCACACCCGCCAGTCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAA‑CGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAggg < 4:684314/149‑1 (MQ=255) aTCCGGCACACCCGCCAGTCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAA‑CGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAg > 1:692796/1‑149 (MQ=255) aTCCGGCACACCCGCCAGTCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAA‑CGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAg > 1:800489/1‑149 (MQ=255) ggCACACCCGCCAGTCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAA‑CGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTc < 1:788496/149‑1 (MQ=255) gCACACCCGCCAGTCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAA‑CGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTcc > 1:863786/1‑149 (MQ=255) cacaCCCGCCAGTCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAA‑CGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCt < 4:887616/149‑1 (MQ=255) cacaCCCGCCAGTCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAA‑CGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCt < 2:294774/149‑1 (MQ=255) cacaCCCGCCAGTCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAA‑CGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCt < 3:349095/149‑1 (MQ=255) ccAGTCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAA‑CGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATaaaa < 3:273367/149‑1 (MQ=255) ccAGTCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAA‑CGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATaaaa < 1:647320/149‑1 (MQ=255) gTCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAA‑CGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATaaaa < 1:732303/146‑1 (MQ=255) gTCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAA‑CGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATaaaa > 2:732303/1‑146 (MQ=255) gTCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAA‑CGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCt < 4:249550/149‑1 (MQ=255) gTCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAA‑CGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCt < 2:73181/149‑1 (MQ=255) cGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAA‑CGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGc < 4:862919/149‑1 (MQ=255) gCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAA‑CGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCa < 4:270832/149‑1 (MQ=255) ccATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAA‑CGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATaaaa > 3:854405/1‑142 (MQ=255) ccATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAA‑CGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATaaaa < 4:854405/142‑1 (MQ=255) ccATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAA‑CGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCaa < 3:26608/149‑1 (MQ=255) cATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAA‑CGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGACATACGGGCGTTCGCCCGATAAAGGGAGCt < 3:793605/129‑1 (MQ=255) cATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAA‑CGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGCGCt > 2:474568/1‑129 (MQ=255) cATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAA‑CGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCt < 1:474568/129‑1 (MQ=255) cATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAA‑CGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCt > 4:793605/1‑129 (MQ=255) gatgCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAA‑CGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGc < 1:787211/149‑1 (MQ=255) tGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAA‑CGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCAcc > 2:447/1‑149 (MQ=255) aCCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAA‑CGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTgg > 2:60333/1‑149 (MQ=255) ccTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAA‑CGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGt < 4:122958/149‑1 (MQ=255) ccTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAA‑CGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGt < 3:563692/149‑1 (MQ=255) cTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAA‑CGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTa > 1:24058/1‑149 (MQ=255) ccAATTTTTACTGACATTCCGCCCAGTCGGTTAA‑CGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACctct > 1:212500/1‑149 (MQ=255) cAATTTTTACTGACATTCCGCCCAGTCGGTTAA‑CGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCtctc > 1:889827/1‑149 (MQ=255) aaTTTTTACTGACATTCCGCCCAGTCGGTTAA‑CGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTAc > 2:816716/1‑143 (MQ=255) aaTTTTTACTGACATTCCGCCCAGTCGGTTAA‑CGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTAc < 1:816716/143‑1 (MQ=255) aaTTTTTACTGACATTCCGCCCAGTCGGTTAA‑CGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCg < 3:82749/149‑1 (MQ=255) tttttACTGACATTCCGCCGAGTCGGTTAA‑CGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGt < 4:630615/149‑1 (MQ=255) tttttACTGACATTCCGCCCAGTCGGTTAA‑CGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGt < 1:48143/149‑1 (MQ=255) ttttACTGACATTCCGCCCAGTCGGTTAA‑CGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGtt < 3:335085/149‑1 (MQ=255) tttACTGACATTCCGCCCAGTCGGTTAA‑CGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGttt < 2:591740/149‑1 (MQ=255) tttACTGACATTCCGCCCAGTCGGTTAA‑CGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGttt > 3:38639/1‑149 (MQ=255) tACTGACATTCCGCCCAGTCGGTTAA‑CGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGa < 2:622563/149‑1 (MQ=255) aCTGACATTCCGCCCAGTCGGTTAA‑CGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGAt > 4:62906/1‑149 (MQ=255) aCATTCCGCCCAGTCGGTTAA‑CGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCg > 4:438523/1‑149 (MQ=255) aCATTCCGCCCAGTCGGTTAA‑CGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCg > 3:839440/1‑149 (MQ=255) aCATTCCGCCCAGTCGGTTAA‑CGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCg > 1:309875/1‑149 (MQ=255) cATTCCGCCCAGTCGGTTAA‑CGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCGa < 2:730334/149‑1 (MQ=255) ttCCGCCCAGTCGGTTAA‑CGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCGaca > 1:725020/1‑149 (MQ=255) gCCCAGTCGGTTAA‑CGACTGCGAAGCGAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCGACAacac > 2:134636/1‑149 (MQ=255) agtcgTTAACCGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCGACAACACACtt > 3:886341/5‑149 (MQ=255) cGGTTAA‑CGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTACCTCACCTTTACTGGTACCTCTCGGTTTGATCTCGACAACACACTTTcc < 4:531141/149‑1 (MQ=255) gTTAA‑CGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGAtctc < 4:652298/132‑1 (MQ=255) gTTAA‑CGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGAtctc > 3:652298/1‑132 (MQ=255) a‑CGACAGCGAAGCCAGAATCATCGGTTGAATCATCGCCCAGAAATACGGGCGTTCGCCCGATGACGGGAGTTTCCTGCACAAAAGCTGCAATTGCCGCACCTTTACTCGTACCTCTCGGTTTGATCTCGACAACACACTTTACctgctg > 2:671899/1‑149 (MQ=255) gACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCGACAACACACTTTCCCTGCTGTa > 3:210010/1‑149 (MQ=255) cGAAGCCAGATTCATCGGTTAAATCATCGCCCAGTAATACGGGCGTTCGCCCGATAAAGGGAGCTTCTTGCATAAATGCTGCAATTGCCTCACCATTACTGGTACCTCTCGGTTTGATCTCGACAACACACTTTCCCTGCTGTAACGcc < 4:925298/149‑1 (MQ=255) cGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCGACAACACACTTTCCCTGCTGTAACGcc > 1:98425/1‑149 (MQ=255) gCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCGACAACACACTTTCCCTGCTGTAACGCCAttt < 2:725020/149‑1 (MQ=255) aGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCGACAACACACTTTCCCTGCTGTAACGCCATTtgtg < 2:692796/149‑1 (MQ=255) aGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCGACAACACACTTTCCCTGCTGTAACGCCATTtgtg < 2:596969/149‑1 (MQ=255) gATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAGGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCGACAACACACTTTCCCTGCTGTAACGCCATTTGTgg < 2:309754/149‑1 (MQ=255) tCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCGACAACACACTTTCCCTGCTGTAACGCCATTTGTGGCCa < 2:98425/149‑1 (MQ=255) aTCGGTTAAATCATCGCCCAGCAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCGCACCTTTACTGGTACCTCTCGGTTTGATCTGGACAACACACTTTCCCTGCTGTAACGCCATTTGTGGCCAGa > 1:670583/1‑149 (MQ=255) aTCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCGACAACACACTTTCCCTGCTGTAACGCCATTTGTGGCCAGa < 2:309165/149‑1 (MQ=255) ggTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCGACAACACACTTTCCCTGCTGTAACGCCATTTGTGGCCAGATCt > 2:145823/1‑149 (MQ=255) gTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCGACAACACACTTTCCCTGCTGTAACGCCATTTGTGGCCAGATCTg < 4:886341/149‑1 (MQ=255) gTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCGACAACACACTTTCCCTGCTGTAACGCCATTTGTGGCCAGATCTg > 2:806800/1‑149 (MQ=255) gTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCGACAACACACTTTCCCTGCTGTAACGCCATTTGTGGCCAGATCTg > 4:394869/1‑149 (MQ=255) ttAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCGACAACACACTTTCCCTGCTGTAACGCCATTTGTGGCCAGATCTGa < 1:288497/149‑1 (MQ=255) catcGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCGACAACACACTTTCCCTGCTGTAACGCCATTTGTGGCCAGATCTGAGTAATa < 2:863786/149‑1 (MQ=255) tcGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCGACAACACACTTTCCCTGCTGTAACGCCATTTGTGGCCAGATCTGAGTAATACg > 1:741487/1‑149 (MQ=255) gCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCGACAACACACTTTCCCTGCTGTAACGCCATTTGTGGCCAGATCTGAGTAATACGtt < 4:599901/149‑1 (MQ=255) cccAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCGACAACACACTTTCCCTGCTGTAACGCCATTTGTGGCCAGATCTGAGTAATACGTTg < 2:497724/149‑1 (MQ=255) ccAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGc < 1:713177/58‑1 (MQ=255) ccAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCGACAACACACTTTCCCTGCTGTAACGCCATTTGTGGCCAGATCTGAGTAATACGTTgc > 4:650616/1‑149 (MQ=255) ccAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCGACAACACACTTTCCCTGCTGTAACGCCATTTGTGGCCAGATCTGAGTAATACGTTgc > 3:119018/1‑149 (MQ=255) caaGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGc > 2:713177/3‑58 (MQ=255) cAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCGACAACACACTTTCCCTGCTGTAACGCCATTTGTGGCCAGATCTGAGTAATACGTTgcg > 3:219886/1‑149 (MQ=255) aGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCGACAACACACTTTCCCTGCTGTAACGCCATTTGTGGCCAGATCTGAGTAATACGTTgcgc < 2:309157/149‑1 (MQ=255) gAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCGac < 1:827930/91‑1 (MQ=255) gAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCGac > 2:827930/1‑91 (MQ=255) ctttatcGGGCGAACGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCGACAACACACTTTCCCTGCTGTAACGCCATTTGTGGCCAGATCTGAGTAATACGTTGCGCt > 1:368181/7‑149 (MQ=255) tACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCGACAACACACTTTCCCTGCTGTAACGCCATTTGTGGCCAGATCTGAGTAATACGTTGCGCTAATg < 2:348724/149‑1 (MQ=255) atcGGGCGAACGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCGACAACACACTTTCCCTGCTGTAACGCCATTTGTGGCCAGATCTGAGTAATACGTTGCGCTAATg > 4:200647/3‑149 (MQ=255) gggCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCGACAACACACTTTCCCTGCTGTAACGCCATTTGTGGCCAGATCTGAGTAATACGTTGCGCTAATGTCa > 3:317647/1‑149 (MQ=255) ggCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCGACAACACACTTTCCCTGCTGTAACGCCATTTGTGGCCAGATCTGAGTAATACGTTGCGCTAATGTCAt < 4:775854/149‑1 (MQ=255) | AAGCCAGCTCCAGACATCCGGCACACCCGCCAGTCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAA‑CGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCGACAACACACTTTCCCTGCTGTAACGCCATTTGTGGCCAGATCTGAGTAATACGTTGCGCTAATGTCAT > NC_000913/1981668‑1981958 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |