Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,999,343 T→G 6.4% A46A (GCA→GCC dcyD ← D‑cysteine desulfhydrase, PLP‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,999,3430TG 6.4% 317.2 / 13.5 94A46A (GCA→GCCdcyDD‑cysteine desulfhydrase, PLP‑dependent
Reads supporting (aligned to +/- strand):  ref base T (47/41);  new base G (4/2);  total (51/43)
Fisher's exact test for biased strand distribution p-value = 6.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.71e-01

CAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTTGATGAAAATTTCCCGTCCTAGATAATCAGAAAAGCGCGGCAGATATTCGAGCGGCGTTGGCGCGCCGATAAACTCCAGCCGTGGAAAACGGGTTAAATTATGCAGTGGCATAACAGCCTC  >  NC_000913/1999202‑1999489
                                                                                                                                             |                                                                                                                                                  
cAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGTACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATgg                                                                                                                                             >  4:47282/1‑149 (MQ=255)
cAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATgg                                                                                                                                             <  3:798003/149‑1 (MQ=255)
  gACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATgggg                                                                                                                                           <  2:866743/149‑1 (MQ=255)
  gACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATgggg                                                                                                                                           <  4:301072/149‑1 (MQ=255)
    ccGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGt                                                                                                                                         <  3:529591/149‑1 (MQ=255)
     cGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTg                                                                                                                                        >  3:20575/1‑149 (MQ=255)
     cGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTg                                                                                                                                        >  4:216253/1‑149 (MQ=255)
       aGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGAc                                                                                                                                      <  3:196710/149‑1 (MQ=255)
         attGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGt                                                                                                                                    >  2:172970/2‑149 (MQ=255)
                  gACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGtt                                                                                                                           <  3:811111/149‑1 (MQ=255)
                      gCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGTATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTtgat                                                                                                                       <  3:435876/149‑1 (MQ=255)
                      gCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTtgat                                                                                                                       <  1:337597/149‑1 (MQ=255)
                      gCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTtgat                                                                                                                       <  2:860014/149‑1 (MQ=255)
                      gCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTtgat                                                                                                                       <  2:423975/149‑1 (MQ=255)
                      gCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTtgat                                                                                                                       <  2:895282/149‑1 (MQ=255)
                         gCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTTGATGaa                                                                                                                    >  2:754769/1‑149 (MQ=255)
                             tCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTTGATGAAAAtt                                                                                                                >  2:542328/1‑149 (MQ=255)
                             tCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTTGATGAAAAtt                                                                                                                >  1:862724/1‑149 (MQ=255)
                               tGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTTGATGAAAATTTc                                                                                                              >  2:206226/1‑149 (MQ=255)
                                gCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTTGATGAAAATTTcc                                                                                                             <  4:221864/149‑1 (MQ=255)
                                gCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTTGATGAAAATTTcc                                                                                                             <  3:310170/149‑1 (MQ=255)
                                     acaTGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTTGATGAAAATTTCCCGTcc                                                                                                        >  1:21930/1‑149 (MQ=255)
                                       aTGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTTGATGAAAATTTCCCGTCCTa                                                                                                      >  3:311328/1‑149 (MQ=255)
                                         ggTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTTGATGAAAATTTCCCGTCCTAGa                                                                                                    <  1:538856/149‑1 (MQ=255)
                                          gTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTTGATGAAAATTTCCCGTCCTAGAt                                                                                                   >  1:806649/1‑149 (MQ=255)
                                           ttAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTTGATGAAAATTTCCCGTCCTAGATa                                                                                                  >  2:607005/1‑149 (MQ=255)
                                           ttAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTTGATGAAAATTTCCCGTCCTAGATa                                                                                                  >  4:813600/1‑149 (MQ=255)
                                             aGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTTGATGAAAATTTCCCGTCCTAGATAAt                                                                                                <  3:33177/149‑1 (MQ=255)
                                              gACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTTGATGAAAATTTCCCGTCCTAGATAATc                                                                                               >  3:531248/1‑149 (MQ=255)
                                                 tGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTTGATGAAAATTTCCCGTCCTAGATAATCAGa                                                                                            >  3:404472/1‑149 (MQ=255)
                                                    aTCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTTGATGAAAATTTCCCGTCCTAGATAATCAGaaaa                                                                                         >  3:117686/1‑149 (MQ=255)
                                                    aTCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTTGATGAAAATTTCCCGTCCTAGATAATCAGaaaa                                                                                         >  1:116289/1‑149 (MQ=255)
                                                        ccccGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTTGATGAAAATTTCCCGTCCTAGATAATCAGAAAAgcgc                                                                                     >  1:737506/1‑149 (MQ=255)
                                                          ccGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTTGATGAAAATTTCCCGTCCTAGATAATCAGAAAAGCGCgg                                                                                   >  2:385668/1‑149 (MQ=255)
                                                              cAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTTGATGAAAATTTCCCGTCCTAGATAATCAGAAAAGCGCGGCAGa                                                                               <  4:478736/149‑1 (MQ=255)
                                                              cAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTTGATGAAAATTTCCCGTCCTAGATAATCAGAAAAGCGCGGCAGa                                                                               <  4:854253/149‑1 (MQ=255)
                                                               aGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTTGATGAAAATTTCCCGTCCTAGATAATCAGAAAAGCGCGGCAGat                                                                              >  1:805493/1‑149 (MQ=255)
                                                                  aaTCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTTGATGAAAATTTCCCGTCCTAGATAATCAGAAAAGCGCGGCAGATAtt                                                                           <  3:296184/149‑1 (MQ=255)
                                                                    tCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTTGATGAAAATTTCCCGTCCTAGATAATCAGAAAAGCGCGGCAGATATTCg                                                                         >  1:122720/1‑149 (MQ=255)
                                                                       gCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTTGATGAAAATTTCCCGTCCTAGATAATCAGAAAAGCGCGGCAGATATTCGAgc                                                                      >  4:302956/1‑149 (MQ=255)
                                                                       gCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTTGATGAAAATTTCCCGTCCTAGATAATCAGAAAAGCGCGGCAGATATTCGAgc                                                                      <  3:91668/149‑1 (MQ=255)
                                                                            tCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTTGATGAAAATTTCCCGTCCTAGATAATCAGAAAAGCGCGGCAGATATTCGAGCGGCGt                                                                 >  2:588026/1‑149 (MQ=255)
                                                                               gCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTTGATGAAAATTTCCCGTCCTAGATAATCAGAAAAGCGCGGCAGATATTCGAGCGGCGTTgg                                                              <  1:606025/149‑1 (MQ=255)
                                                                               gCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTTGATGAAAATTTCCCGTCCTAGATAATCAGAAAAGCGCGGCAGATATTCGAGCGGCGTTgg                                                              >  2:736517/1‑149 (MQ=255)
                                                                                   cTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTTGATGAAAATTTCCCGTCCTAGATAATCAGAAAAGCGCGGCAGATATTCGAGCGGCGTTg                                                               <  4:23275/144‑1 (MQ=255)
                                                                                   cTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTTGATGAAAATTTCCCGTCCTAGATAATCAGAAAAGCGCGGCAGATATTCGAGCGGCGTTg                                                               >  3:23275/1‑144 (MQ=255)
                                                                                    ttCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTTGATGAAAATTTCCCGTCCTAGATAATCAGAAAAGCGCGGCAGATATTCGAGCGGCGTTGgcgcgc                                                         >  2:443660/1‑149 (MQ=255)
                                                                                    ttCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTTGATGAAAATTTCCCGTCCTAGATAATCAGAAAAGCGCGGCAGATATTCGAGCGGCGTTGgcgcgc                                                         >  3:726398/1‑149 (MQ=255)
                                                                                        cGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTTGATGAAAATTTCCCGTCCTAGATAATCAGAAAAGCGCGGCAGATATTCGAGCGGCGTTGGCGCGCCGAt                                                     >  2:186889/1‑149 (MQ=255)
                                                                                         gCAGAGCATCTGCCGCGCGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTTGATGAAAATTTCCCGTCCTAGATAATCAGAAAAGCGCGGCAGATATTCGAGCGGCGTTGGCGCGCCGATa                                                    >  4:850529/1‑149 (MQ=255)
                                                                                         gCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTTGATGAAAATTTCCCGTCCTAGATAATCAGAAAAGCGCGGCAGATATTCGAGCGGCGTTGGCGCGCCGATa                                                    >  4:699117/1‑149 (MQ=255)
                                                                                         gCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTTGATGAAAATTTCCCGTCCTAGATAATCAGAAAAGCGCGGCAGATATTCGAGCGGCGTTGGCGCGCCGATa                                                    >  4:50950/1‑149 (MQ=255)
                                                                                         gCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTTGATGAAAATTTCCCGTCCTAGATAATCAGAAAAGCGCGGCAGATATTCGAGCGGCGTTGGCGCGCCGATa                                                    >  3:818684/1‑149 (MQ=255)
                                                                                         gCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGAAGTCATCCCGTTTGATGAAAATTTCCCGTCCTAGATAATCAGAAAAGCGCGGCAGATATTCGAGCGGCGTTGGCGCGCCGATa                                                    >  3:722399/1‑149 (MQ=255)
                                                                                            gagCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTTGATGAAAATTTCCCGTCCTAGATAATCAGAAAAGCGCGGCAGATATTCGAGCGGCGTTGGCGCGCCGATAAAc                                                 >  2:197000/1‑149 (MQ=255)
                                                                                               cATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTTGATGAAAATTTCCCGTCCTAGATAATCAGAAAAGCGCGGCAGATATTCGAGCGGCGTTGGCGCGCCGATAAACTcc                                              >  2:874975/1‑149 (MQ=255)
                                                                                                aTCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTTGATGAAAATTTCCCGTCCTAGATAATCAGAAAAGCGCGGCAGATATTCGAGCGGCGTTGGCGCGCCGATAAACTCCa                                             <  2:116289/149‑1 (MQ=255)
                                                                                                 tCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTTGATGAAAATTTCCCGTCCTAGATAATCAGAAAAGCGCGGCAGATATTCGAGCGGCGTTGGCGCGCCGATAAACTCCAg                                            <  1:639914/149‑1 (MQ=255)
                                                                                                     ccGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTTGATGAAAATTTCCCGTCCTAGATAATCAGAAAAGCGCGGCAGATATTCGAGCGGCGTTGGCGCGCCGATAAACTCCAGCCGt                                        <  2:862724/149‑1 (MQ=255)
                                                                                                       gcgAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTTGATGAAAATTTCCCGTCCTAGATAATCAGAAAAGCGCGGCAGATATTCGAGCGGCGTTGGCGCGCCGATAAACTCCAGCCGTgg                                      <  1:687444/149‑1 (MQ=255)
                                                                                                           gaAATTCCGGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTTGATGAAAATTTCCCGTCCTAGATAATCAGAAAAGCGCGGCAGATATTCGAGCGGCGTTGGCGCGCCGATAAACTCCAGCCGTGGaaaa                                  >  4:80095/1‑149 (MQ=255)
                                                                                                              aTTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTTGATGAAAATTTCCCGTCCTAGATAATCAGAAAAGCGCGGCAGATATTCGAGCGGCGTTGGCGCGCCGATAAACTCCAGCCGTGGAAAACgg                               <  3:845711/149‑1 (MQ=255)
                                                                                                              aTTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTTGATGAAAATTTCCCGTCCTAGATAATCAGAAAAGCGCGGCAGATATTCGAGCGGCGTTGGCGCGCCGATAAACTCCAGCCGTGGAAAACgg                               <  2:806649/149‑1 (MQ=255)
                                                                                                              aTTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTTGATGAAAATTTCCCGTCCTAGATAATCAGAAAAGCGCGGCAGATATTCGAGCGGCGTTGGCGCGCCGATAAACTCCAGCCGTGGAAAACgg                               <  1:172970/149‑1 (MQ=255)
                                                                                                                tCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTTGATGAAAATTTCCCGTCCTAGATAATCAGAAAAGCGCGGCAGATATTCGAGCGGCGTTGGCGCGCCGATAAACTCCAGCCGTGGAAAACGGGt                             <  3:610812/149‑1 (MQ=255)
                                                                                                                tCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTTGATGAAAATTTCCCGTCCTAGATAATCAGAAAAGCGCGGCAGATATTCGAGCGGCGTTGGCGCGCCGATAAACTCCAGCCGTGGAAAACGGGt                             <  1:896776/149‑1 (MQ=255)
                                                                                                                    gCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTTGATGAAAATTTCCCGTCCTAGATAATCAGAAAAGCGCGGCAGATATTCGAGCGGCGTTGGCGCGCCGATAAACTCCAGCCGTGGAAAACGGGTTaaa                         >  4:35241/1‑149 (MQ=255)
                                                                                                                     cTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTTGATGAAAATTTCCCGTCCTAGATAATCAGAAAAGCGCGGCAGATATTCGAGCGGCGTTGGCGCGCCGATAAACTCCAGCCGTGGAAAACGGGTTAAAt                        <  4:404472/149‑1 (MQ=255)
                                                                                                                       tACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTTGATGAAAATTTCCCGTCCTAGATAATCAGAAAAGCGCGGCAGATATTCGAGCGGCGTTGGCGCGCCGATAAACTCCAGCCGTGGAAAACGGGTTAAATTa                      <  4:531248/149‑1 (MQ=255)
                                                                                                                        aCGTAATTTATTGCCGCCCATTGCCATGGGAGTGACGTCATCCCGTTTGATGAAAATTTCCCGTCCTAGATAATCAGAAAAGCGCGGCAGATATTCGAGCGGCGTTGGCGCGCCGATAAACTCCAGCCGTGGAAAACGGGTTAAATTAt                     <  1:607005/149‑1 (MQ=255)
                                                                                                                         cGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTTGATGAAAATTTCCCGTCCTAGATAATCAGAAAAGCGCGGCAGATATTCGAGCGGCGTTGGCGCGCCGATAAACTCCAGCCGTGGAAAACGGGTTAAATTATg                    <  2:122720/149‑1 (MQ=255)
                                                                                                                          gTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTTGATGAAAATTTCCCGTCCTAGATAATCAGAAAAGCGCGGCAGATATTCGAGCGGCGTTGGCGCGCCGATAAACTCCAGCCGTGGAAAACGGGTTAAATTATGc                   >  2:637986/1‑149 (MQ=255)
                                                                                                                           tAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTATGATGAAAATTTCCCGTCCTAGATACTCGGAAAAGCGCGGCAGATATTCGAGCGGCGTTGGCGCGCCGATAAACTCCAGCCGTGGAAAACGGGTTAAATTATGcc                  >  3:233283/1‑148 (MQ=255)
                                                                                                                             aTTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTTGATGAAAATTTCCCGTCCTAGATAATCAGAAAAGCGCGGCAGATATTCGAGCGGCGTTGGCGCGCCGATAAACTCCAGCCGTGGAAAACGGGTTAAATTATGCAGt                >  2:128469/1‑149 (MQ=255)
                                                                                                                             aTTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTTGATGAAAATTTCCCGTCCTAGATAATCAGAAAAGCGCGGCAGATATTCGAGCGGCGTTGGCGCGCCGATAAACTCCAGCCGTGGAAAACGGGTTAAATTATGCAGt                >  4:118468/1‑149 (MQ=255)
                                                                                                                              tttATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTTGATGAAAATTTCCCGTCCTAGATAATCAGAAAAGCGCGGCAGATATTCGAGCGGCGTTGGCGCGCCGATAAACTCCAGCCGTGGAAAACGGGTTAAATTATGCAGTg               >  2:257764/1‑149 (MQ=255)
                                                                                                                              tttATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTTGATGAAAATTTCCCGTCCTAGATAATCAGAAAAGCGCGGCAGATATTCGAGCGGCGTTGGCGCGCCGATAAACTCCAGCCGTGGAAAACGGGTTAAATTATGCAGTg               <  3:118468/149‑1 (MQ=255)
                                                                                                                                tATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTTGATGAAAATTTCCCGTCCTAGATAATCAGAAAAGCGCGGCAGATATTCGAGCGGCGTTGGCGCGCCGATAAACTCCAGCCGTGGAAAACGGGTTAAATTATGCAGTGGc             <  2:736005/149‑1 (MQ=255)
                                                                                                                                tATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTTGATGAAAATTTCCCGTCCTAGATAATCAGAAAAGCGCGGCAGATATTCGAGCGGCGTTGGCGCGCCGATAAACTCCAGCCGTGGAAAACGGGTTAAATTATGCAGTGGc             <  3:216253/149‑1 (MQ=255)
                                                                                                                                   tcacccccATGGCAATGGGGGTGACGTCATCCCGTTTGATGAAAATTTCCCGTCCTAGATAATCAGAAAAGCGCGGCAGATATTCGAGCGGCGTTGgcgc                                                           >  2:757387/6‑100 (MQ=255)
                                                                                                                                   tcacccccATGGCAATGGGGGTGACGTCATCCCGTTTGATGAAAATTTCCCGTCCTAGATAATCAGAAAAGCGCGGCAGATATTCGAGCGGCGTTGgcgc                                                           <  1:757387/95‑1 (MQ=255)
                                                                                                                                   tcacccccATGGCAATGGGGGTGACGTCATCCCGTTTGATGAAAATTTCCCGTCCTAGATAATCAGAAAAGCGCGGCAGATATTCGAGCGGCGTTGGCGCGCCGATAAACTCCAGCCGTGGAAAACGGGTTAAATTATGCAGTgg              <  3:557748/140‑1 (MQ=255)
                                                                                                                                   tcacccccATGGCAATGGGGGTGACGTCATCCCGTTTGATGAAAATTTCCCGTCCTAGATAATCAGAAAAGCGCGGCAGATATTCGAGCGGCGTTGGCGCGCCGATAAACTCCAGCCGTGGAAAACGGGTTAAATTATGCAGTgg              >  4:557748/6‑145 (MQ=255)
                                                                                                                                    cacccccATGGCAATGGGGGTGACGTCATCCCGTTTGATGAAAATTTCCCGTCCTAGATAATCAGAAAAGCGCGGCAGATATTCGAGCGGCGTTGGCGCGCCGATAAACTCCAGCCGTGGAAAACGGGTTAAATTATGCAGTGGCATaa         >  1:514419/5‑149 (MQ=255)
                                                                                                                                     ccgccCATTGCCATGGGGGTGACGTCATCCCGTTTGATGAAAATTTCCCGTCCTAGATAATCAGAAAAGCGCGGCAGATATTCGAGCGGCGTTGGCGCGCCGATAAACTCCAGCCGTGGAAAACGGGTTAAATTATGCAGTGGCATAAc        <  2:178456/149‑1 (MQ=255)
                                                                                                                                      cgccCATTGCCATGGGGGTGACGTCATCCCGTTTGATGAAAATTTCCCGTCCTAGATAATCAGAAAAGCGCGGCAGATATTCGAGCGGCGTTGGCGCGCCGATAAACTCCAGCCGTGGAAAACGGGTTAAATTATGCAGTGGCATAACa       <  1:123991/149‑1 (MQ=255)
                                                                                                                                       gccCATTGCCATGGGGGTGACGTCATCCCGTTTGATGAAAATTTCCCGTCCTAGATAATCAGAAAAGCGCGGCAGATATTCGAGCGGCGTTGGCGCGCCGATAAACTCCAGCCGTGGAAAACGGGTTAAATTATGCAGTGGCATAACAg      >  3:769854/1‑149 (MQ=255)
                                                                                                                                       ccccATGGCAATGGGGGTGACGTCATCCCGTTTGATGAAAATTTCCCGTCCTAGATAATCAGAAAAGCGCGGCAGATATTCGAGCGGCGTTGGCGCGCCGATAAACTCCAGCCGTGGAAAACGGGTTAAATTATGCAGTGGCATAACAg      >  1:99460/2‑149 (MQ=255)
                                                                                                                                        cccATTGCCATGGGGGTGACGTCATCCCGTTTGATGAAAATTTCCCGTCCTAGATAATCAGAAAAGCGCGGCAGATATTCGAGCGGCGTTGGCGCGCCGATAAACTCCAGCCGTGGAAAACg                                <  4:503758/122‑1 (MQ=255)
                                                                                                                                        cccATTGCCATGGGGGTGACGTCATCCCGTTTGATGAAAATTTCCCGTCCTAGATAATCAGAAAAGCGCGGCAGATATTCGAGCGGCGTTGGCGCGCCGATAAACTCCAGCCGTGGAAAACg                                >  3:503758/1‑122 (MQ=255)
                                                                                                                                         ccATTGCCATGGGGGTGACGTCATCCCGTTTGATGAAAATTTCCCGTCCTAGATAATCAGAAAAGCGCGGCAGATATTCGAGCGGCGTTGGCGCGCCGATAAACTCCAGCCGTGGAAAACGGGTTAAATTATg                    <  2:480309/133‑1 (MQ=255)
                                                                                                                                         ccATTGCCATGGGGGTGACGTCATCCCGTTTGATGAAAATTTCCCGTCCTAGATAATCAGAAAAGCGCGGCAGATATTCGAGCGGCGTTGGCGCGCCGATAAACTCCAGCCGTGGAAAACGGGTTAAATTATg                    >  1:480309/1‑133 (MQ=255)
                                                                                                                                         ccATTGCCATGGGGGTGACGTCATCCCGTTTGATGAAAATTTCCCGTCCTAGATAATCAGAAAAGCGCGGCAGATATTCGAGCGGCGTTGGCGCGCCGATAAACTCCAGCCGTGGAAAACGGGTTAAATTATGCAGTGGCATAACAGcc    >  3:852007/1‑149 (MQ=255)
                                                                                                                                           aTTGCCATGGGGGTGACGTCATCCCGTTTGATGAAAATTTCCCGTCCTAGATAATCAGAAAAGCGCGGCAGATATTCGAGCGGCGTTGGCGCGCCGATAAACTCCAGCCGTGGAAAACGGGTTAAATTATGCAGTGGCATAACAGCCTc  >  4:505081/1‑149 (MQ=255)
                                                                                                                                             |                                                                                                                                                  
CAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTTGATGAAAATTTCCCGTCCTAGATAATCAGAAAAGCGCGGCAGATATTCGAGCGGCGTTGGCGCGCCGATAAACTCCAGCCGTGGAAAACGGGTTAAATTATGCAGTGGCATAACAGCCTC  >  NC_000913/1999202‑1999489

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: