| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,014,396 | G→T | 5.4% | D390Y (GAT→TAT) ‡ | fliF → | flagellar basal‑body MS‑ring and collar protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,014,396 | 0 | G | T | 5.4% | 373.6 / 6.8 | 106 | D390Y (GAT→TAT) ‡ | fliF | flagellar basal‑body MS‑ring and collar protein |
| Reads supporting (aligned to +/- strand): ref base G (46/54); new base T (3/3); total (49/57) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 106 total aligned reads displayed.
ACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGTCATACCAAAATGAACGTGGGCGATGTGCAACGTCTGTCAGTCGCGGTCGTGGTGAATTACAAAACCTTGCCAGATGGCAAACCGTTGCCTCTCAGCAACGAACAGATGAAGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCAGTGACGAAAGCGGCG > NC_000913/2014249‑2014537 | accaccAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGTCATACCAAAATGAACGTGGGCGATGTGCAACGTCTGTCAGTCGCGGTCGTGGTGAATTACAAAACCTTGCCAGa < 3:74893/149‑1 (MQ=255) ccaccaGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGTCATACCAAAATGAACGTGGGCGATGTGCAACGTCTGTCAGTCGCGGTCGTGGTGAATTACAAAACCTTGCCAGAt < 2:652596/149‑1 (MQ=255) caccaGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGTCATACCAAAATGAACGTGGGCGATGTGCAACGTCTGTCAGTCGCGGTCGTGGTGAATTACAAAACCTTGCCAGATg > 1:779582/1‑149 (MQ=255) ccaGCAAAAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGTCATACCAAAATGAACGTGGGCGATGTGCAACGTCTGTCAGTCGCGGTCGTGGTGAATTACAAAACCTTGCCAGATGGc < 2:657034/149‑1 (MQ=255) ccaGCAAAAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGTCATACCAAAATGAACGTGGGCGATGTGCAACGTCTGTCAGTCGCGGTCGTGGTGAATTACAAAACCTTGCAAGATGGc < 2:507756/149‑1 (MQ=255) aGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGTCATACCAAAATGAACGTGGGCGATGTGCAACGTCTGTCAGTCGCGGTCGTGGTGAATTACAAAACCTTGCCAGATGGCaa > 1:125991/1‑149 (MQ=255) aTAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGTCATACCAAAATGAACGTGGGCGATGTGCAACGTCTGTCAGTCGCGGTCGTGGTGAATTACAAAACCTTGCCATCTGGCaaggtt < 2:190210/149‑5 (MQ=255) tGGGCCGCGTAGCACACAGCGGAATGAAACCCGTAACTACGAAGTCGATCGCACCATTCGTCATACCAAAATGAACGTGGGCGATGTGCAACGTCTGTCAGTCGCGGTCGTGGTGAATTACAAAACCTTGCCAGATGGCAAACCGTTGc < 4:90563/149‑1 (MQ=255) ggCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGTCATACCAAAATGAACGTGGGCGATGTGCAACGTCTGTCAGTCGCGGTCGTGGTGAATTACAAAACCTTGCCATCTGGCaaggtttt < 3:365515/145‑7 (MQ=255) ggCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGTCATACCAAAATGAACGTGGGCGATGTGCAACGTCTGTCAGTCGCGGTCGTGGTGAATTACAAAACCTTGCCATCTGGCaaggtttt > 4:365515/1‑139 (MQ=255) ggCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGTCATACCAAAATGAACGTGGGCGATGTGCAACGTCTGTCAGTCGCGGTCGTGGTGAATTACAAAACCTTGCCAGATGGCAAACCGTTGCct > 3:250031/1‑149 (MQ=255) tAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGTCATACCAAAATGAACGTGGGCGATGTGCAACGTCTGTCAGTCGCGGTCGTGGTGAATTACAAAACCTTGCCAGATGGCAAACCGTTGCCTCTCAGCa > 3:898298/1‑149 (MQ=255) cacaGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGTCATACCAAAATGAACGTGGGCGATGTGCAACGTCTGTCAGTCGCGGTCGTGGTGAATTACAAAACCTTGCCAGATGGCAAACCGTTGCCTCTCAGCAACGaa < 4:748831/149‑1 (MQ=255) gCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGTCATACCAAAATGAACGTGGGCGATGTGCAACGTCTGTCAGTCGCGGTCGTGGTGAATTACAAAACCTTGCCAGATGGCAAACCGTTGCCTCTCAGCAACGAACAGa < 2:287744/149‑1 (MQ=255) cGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGTCATACCAAAATGAACGTGGGCGATGTGCAACGTCTGTCAGTCGCGGTCGTGGTGAATTACAAAACCTTGCCAGATGGCAAACCGTTGCCTCTCAGCAACGAACAGAt < 4:377084/149‑1 (MQ=255) gAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGTCATACCAAAATGAACGTGGGCGATGTGCAACGTCTGTCAGTCGCGGTCGTGGTGAATTACAAAACCTTGCCAGATGGCAAACCGTTGCCTCTCAGCAACGAACAGATGa < 2:36686/149‑1 (MQ=255) aaTGAAACCAGTAACTACGAAGTCGATCGCACCATTCGTCATACCAAAATGAACGTGGGCGATGTGCAACGTCTGTCAGTCGCGGTCGTGGTGAATTACAAAACCTTGCCAGATGGCAAACCGTTGCCTCTCAGCAACGAACAGATGaa < 2:700359/149‑1 (MQ=255) aaTGAAACCAGTAACTACGAAGTCGATCGCACCATTCGTCATACCAAAATGAACGTGGGCGATGTGCAACGTCTGTCAGTCGCGGTCGTGGTGAATTACAAAACCTTGCCAGATGGCAAACCGTTGCCTCTCAGCAACGAACAGATGaa < 3:824077/149‑1 (MQ=255) aTGAAACCAGTAACTACGAAGTCGATCGCCCCATTCGTCATACCAAAATGAACGTGGGCGATGTGCCACCTCTGTCAGTCGCGGTCGTGGTGAATTACAAAACCTTGCCAGATGGCaaa < 3:926122/119‑1 (MQ=255) aTGAAACCAGTAACTACGAAGTCGATCGCACCATTCGTCATACCAAAATGAACGTGGGCGATGTGCAACGTCTGTCAGTCGCGGTCGTGGTGAATTACAAAACCTTGCCAGATGGCAAACCGTTGCCTCTCAGCAACGAACAGATGAAg < 4:427119/149‑1 (MQ=255) aaaCCAGTAACTACGAAGTCGATCGCACCATTCGTCATACCAAAATGAACGTGGGCGATGTGCAACGTCTGTCAGTCGCGGTCGTGGTGAATTACAAAACCTTGCCAGATGGCAAACCGTTGCCTCTCAGCAACGAACAGATGAAGCaa > 2:620212/1‑149 (MQ=255) aCCAGTAACTACGAAGTCGATCGCACCATTCGTCATACCAAAATGAACGTGGGCGATGTGCAACGTCTGTCAGTCGCGGTCGTGGTGAATTACAAAACCTTGCCAGATGGCAAACCGTTGCCTCTCAGCAACGAACAGATGAAGCAAAt < 2:168163/149‑1 (MQ=255) ccAGTAACTACGAAGTCGATCGCACCATTCGTCATACCAAAATGAACGTGGGCGATGTGCAACGTATGTCAGTCGCGGTCGTGGTGAATTACAAAACCTTGCCAGATGGCAAACCGTTGCCTCTCAGCAACGAACAGATGAAGCAAAtt < 4:523871/149‑1 (MQ=255) gTAACTACGAAGTCGATCGCACCATTCGTCATACCAAAATGAACGTGGGCGATGTGCAACGTCTGTCAGTCGCGGTCGTGGTGAATTACAAAACCTTGCCAGATGGCAAACCGTTGCCTCTCAGCAACGAACAGATGAAGCAAATTGaa < 1:314860/149‑1 (MQ=255) aaCTACGAAGTCGATCGCACCATTCGTCATACCAAAATGAACGTGGGCGATGTGCAACGTCTGTCAGTCGCGGTCGTGGTGAATTACAAAACCTTGCCAGATGGCAAACCGTTGCCTCTCAGCAACGAACAGAt < 2:507760/134‑1 (MQ=255) aaCTACGAAGTCGATCGCACCATTCGTCATACCAAAATGAACGTGGGCGATGTGCAACGTCTGTCAGTCGCGGTCGTGGTGAATTACAAAACCTTGCCAGATGGCAAACCGTTGCCTCTCAGCAACGAACAGAt > 1:507760/1‑134 (MQ=255) tACGAAGTCGATCGCACCATTCGTCATACCAAAATGAACGTGGGCGATGTGCAACGTCTGTCAGTCGCGGTCGTGGTGAATTACAAAACCTTGCCAGATGGCAAACCGTTGCCTCTCAGCAACGAACAGATGAAGCAAATTGAAGATCt > 3:363623/1‑149 (MQ=255) tACGAAGTCGATCGCACCATTCGTCATACCAAAATGAACGTGGGCGATGTGCAACGTCTGTCAGTCGCGGTCGTGGTGAATTACAAAACCTTGCCAGATGGCAAACCGTTGCCTCTCAGCAACGAACAGATGAAGCAAATTGAAGATCt > 1:600326/1‑149 (MQ=255) tACGAAGTCGATCGCACCATTCGTCATACCAAAATGAACGTGGGCGATGTGCAACGTCTGTCAGTCGCGCTCGTGGTGAATTACAAAACCTTGCCAGATGGCAAACCGTTGCCTCTCAGCAACGAACAGATGAAGCAAATTGAAGATCt > 1:933548/1‑149 (MQ=255) cGAAGTCGATCGCACCATTCGTCATACCAAAATGAACGTGGGCGATGTGCAACGTCTGTCAGTCGCGGTCGTGGTGAATTACAAAACCTTGCCAGATGGCAAACCGTTGCCTCTCAGCAACGAACAGATGAAGCAAATTGAAGATCTGa < 4:616827/149‑1 (MQ=255) cGAAGTCGATCGCACCATTCGTCATACCAAAATGAACGTGGGCGATGTGCAACGTCTGTCAGTCGCGGTCGTGGTGAATTACAAAACCTTGCCAGATGGCAAACCGTTGCCTCTCAGCAACGAACAGATGAAGCAAATTGAAGATCTGa < 2:600326/149‑1 (MQ=255) gAAGTCGATCGCACCATTCGTCATACCAAAATGAACGTGGGCGATGTGCAACGTCTGTCAGTCGCGGTCGTGGTGAATTACAAAACCTTGCCAGATGGCAAACCGTTGCCTCTCAGCAACGAACAGATGAAGCAAATTGAAGATCTGAc < 3:612148/149‑1 (MQ=255) gAAGTCGATCGCACCATTCGTCATACCAAAATGAACGTGGGCGATGTGCAACGTCTGTCAGTCGCGGTCGTGGTGAATTACAAAACCTTGCCAGATGGCAAACCGTTGCCTCTCAGCAACGAACAGATGAAGCAAATTGAAGATCTGAc > 3:365695/1‑149 (MQ=255) aGTCGATCGCACCATTCGTCATACCAAAATGAACGTGGGCGATGTGCAACGTCTGTCAGTCGCGGTCGTGGTGAATTACAAAACCTTGCCAGATGGCAAACCGTTGCCTCTCAGCAACGaa > 1:421458/1‑121 (MQ=255) aGTCGATCGCACCATTCGTCATACCAAAATGAACGTGGGCGATGTGCAACGTCTGTCAGTCGCGGTCGTGGTGAATTACAAAACCTTGCCAGATGGCAAACCGTTGCCTCTCAGCAACGaa < 2:421458/121‑1 (MQ=255) gATCGCACCATTCGTCATACCAAAATGAACGTGGGCGATGTGCAACGTCTGTCAGTCGCGGTCGTGGTGAATTACAAAACCTTGCCAGATGGCAAACCGTTGCCTCTCAGCAACGAACAGATGAAGCAAATTGAAGATCTGACCCGCGa < 4:898298/149‑1 (MQ=255) aTCGCACCATTCGTCATACCAAAATGAACGTGGGCGATGTGCAACGTCTGTCAGTCGCGGTCGTGGTGAATTACAAAACCTTGCCAGATGGCAAACCGTTGCCTCTCAGCAACGAACAGATGAAGCAAATTGAAGATCTGACCCGCGAg > 3:741572/1‑149 (MQ=255) aTCGCACCATTCGTCATACCAAAATGAACGTGGGCGATGTGCAACGTCTGTCAGTCGCGGTCGTGGTGAATTACAAAACCTTGCCAGATGGCAAACCGTTGCCTCTCAGCAACGAACAGATGAAGCAAATTGAAGATCTGACCCGCGAg > 2:927813/1‑149 (MQ=255) gCACCATTCGTCATACCAAAATGAACGTGGGCGATGTGCAACGTCTGTCAGTCGCGGTCGTGGTGAATTACAAAACCTTGCCAGATGGCAAACCGTTGCCTCTCAGCAACGAACAGATGAAGCAAATTGAAGATCTGACCCGCGAGGCg < 4:559988/149‑1 (MQ=255) aTTCGTCATACCAAAATGAACGTGGGCGATGTGCAACGTCTGTCAGTCGCGGTCGTGGTGAATTACAAAACCTTGCCAGATGGCAAACCGTTGCCTCTCAGCAACGAACAGATGAAGCAAATTGAAGATCTGACCCGCGAGGCGATggg > 4:466318/1‑149 (MQ=255) ttCGTCATACCAAAATGAACGTGGGCGATGTGCAACGTCTGTCAGTCGCGGTCGTGGTGAATTACAAAACCTTGCCAGATGGCAAACCGTTGCCTCTCAGCAACGAACAGATGAAGCAAATTGAAGATCTGACCCGCGAGGCGATGGGc > 1:522308/1‑149 (MQ=255) gTCATACCAAAATGAACGTGGGCGATGTGCAACGTCTGTCAGTCGCGGTCGTGGTGAATTACAAAACCTTGCCAGATGGCAAACCGTTGCCTCTCAGCAACGAACAGATGAAGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCttt > 2:188864/1‑149 (MQ=255) gTCATACCAAAATGAACGTGGGCGATGTGCAACGTCTGTCAGTCGCGGTCGTGGTGAATTACAAAACCTTGCCAGATGGCAAACCGTTGCCTCTCAGCAACGAACAGATGAAGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCttt > 3:274948/1‑149 (MQ=255) tCATACCAAAATGAACGTGGGCGATGTGCAACGTCTGTCAGTCGCGGTCGTGGTGAATTACAAAACCTTGCCAGATGGCAAACCGTTGCCTCTCAGCAACGAACAGATGAAGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCtttt < 4:66262/149‑1 (MQ=255) tCATACCAAAATGAACGTGGGCGATGTGCAACGTCTGTCAGTCGCGGTCGTGGTGAATTACAAAACCTTGCCAGATGGCAAACCGTTGCCTCTCAGCAACGAACAGATGAAGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCtttt < 3:150120/149‑1 (MQ=255) aTACCAAAATGAACGTGGGCGATGTGCAACGTCTGTCAGTCGCGGTCGTGGTGAATTACAAAACCTTGCCAGATGGCAAACCGTTGCCTCTCAGCAACGAACAGATGAAGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCt > 1:120350/1‑149 (MQ=255) cAAAATGAACGTGGGCGATGTGCAACTGCTGTCAGTCGCGGTCGTGGTAAATTACAAAACCTTGCCAGATTGCAAACCGTTGCCTCTCAGGAACGAACAGATGAAGCAAAGTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGaaa > 4:340104/1‑149 (MQ=255) cAAAATGAACGTGGGCGATGTGCAACGTCTGTCAGTCGCGGTCGTGGTGAATTACAAAACCTTGCCAGATGGCAAACCGTTGCCTCTCAGCAACGAACAGATGAAGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGaaa > 1:415304/1‑149 (MQ=255) cAAAATGAACGTGGGCGATGTGCAACGTCTGTCAGTCGCGGTCGTGGTGAATTACAAAACCTTGCCAGATGGCAAACCGTTGCCTCTCAGCAACGAACAGATGAAGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGaaa > 2:863243/1‑149 (MQ=255) cAAAATGAACGTGGGCGATGTGCAACGTCTGTCAGTCGCGGTCGTGGTGAATTACAAAACCTTGCCAGATGGCAAACCGTTGCCTCTCAGCAACGAACAGATGAAGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGaaa > 3:38593/1‑149 (MQ=255) aaaaTGAACGTGGGCGATGTGCAACGTCTGTCAGTCGCGGTCGTGGTGAATTACAAAACCTTGCCAGATGGCAAACCGTTGCCTCTCAGCAACGAACAGATGAAGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGaaaa > 1:724365/1‑149 (MQ=255) gAACGTGGGCGATGTGCAACGTCTGTCAGTCGCGGTCGTGGTGAATTACAAAACCTTGCCAGATGGCAAACCGTTGCCTCTCAGCAACGAACAGATGAAGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAAcgcg < 2:702165/149‑1 (MQ=255) aCGTGGGCGATGTGCAACGTCTGTCAGTCGCGGTCGTGGTGAATTACAAAACCTTGCCAGATGGCAAACCGTTGCCTCTCAGCAACGAACAGATGAAGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGt < 4:363623/149‑1 (MQ=255) aCGTGGGCGATGTGCAACGTCTGTCAGTCGCGGTCGTGGTGAATTACAAAACCTTGCCAGATGGCAAACCGTTGCCTCTCAGCAACGAACAGATGAAGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGt < 3:248173/149‑1 (MQ=255) tGGGCGATGTGCAACGTCTGTCAGTCGCGGTCGTGGTGAATTACAAAACCTTGCCAGATGGCAAACCGTTGCCTCTCAGCAACGAACAGATGAAGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGa < 2:869368/148‑1 (MQ=255) tGGGCGATGTGCAACGTCTGTCAGTCGCGGTCGTGGTGAATTACAAAACCTTGCCAGATGGCAAACCGTTGCCTCTCAGCAACGAACAGATGAAGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGa > 1:869368/1‑148 (MQ=255) tGGGCGATGTGCAACGTCTGTCAGTCGCGGTCGTGGTGAATTACAAAACCTTGCCAGATGGCAAACCGTTGCCTCTCAGCAACGAACAGATGAAGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGAc > 4:430194/1‑149 (MQ=255) gggCGATGTGCAACGTCTGTCAGTCGCGGTCGTGGTGAATTACAAAACCTTGCCAGATGGCAAACCGTTGCCTCTCAGCAACGAACAGATGAAGCAAATTGAAGATCTGACCCGCGAGGCGa > 1:194149/1‑122 (MQ=255) gggCGATGTGCAACGTCTGTCAGTCGCGGTCGTGGTGAATTACAAAACCTTGCCAGATGGCAAACCGTTGCCTCTCAGCAACGAACAGATGAAGCAAATTGAAGATCTGACCCGCGAGGCGa < 2:194149/122‑1 (MQ=255) ggCGATGTGCAACGTCTGTCAGTCGCGGTCGTGGTGAATTACAAAACCTTGCCAGATGGCAAACCGTTGCCTCTCAGCAACGAACAGATGAAGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTc < 3:821222/149‑1 (MQ=255) ggCGATGTGCAACGTCTGTCAGTCGCGGTCGTGGTGAATTACAAAACCTTGCCAGATGGCAAACCGTTGCCTCTCAGCAACGAACAGATGAAGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTc < 3:623498/149‑1 (MQ=255) gATGTGCAACGTCTGTCAGTCGCGGTCGTGGTGAATTACAAAACCTTGCCAGATGGCAAACCGtt > 1:38243/1‑65 (MQ=255) tgtgCAACGTCTGTCAGTCGCGGTCGTGGTGAATTACAAAACCTTGCCAGATGGCAAACCGTTGCCTCTCAGCAACGAACAGATGAAGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCa > 4:803643/1‑149 (MQ=255) tgtgCAACGTCTGTCAGTCGCGGTCGTGGTGAATTACAAAACCTTGCCAGATGGCAAACCGTTGCCGCTCAGCAACGAACAGATGAAGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCa < 2:466085/149‑1 (MQ=255) gtgCAACGTCTGTCAGTCGCGGTCGTGGTGAATTACAAAACCTTGCCAGATGGCAAACCGTTGCCTCTCAGCAACGAACAGATGAAGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCaa < 4:241761/149‑1 (MQ=255) tgCAACGTCTGTCAGTCGCGGTCGTGGTGAATTACAAAACCTTGCCAGATGGCAAACCGTTGCCTCTCAGCAACGAACAGATGAAGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAAt > 2:609255/1‑149 (MQ=255) tgCAACGTCTGTCAGTCGCGGTCGTGGTGAATTACAAAACCTTGCCAGATGGCAAACCGTTGCCTCTCAGCAACGAACAGATGAAGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAAt < 3:313650/149‑1 (MQ=255) cAACGGCTGTCAGTCGCGGTCGTGGTGAATTACAAAACCTTGCCAGATGGCAAACCGTTGCCTCTCAGCAACGAACAGATGAAGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGt > 2:71565/1‑149 (MQ=255) aaCGTCTGTCAGTCGCGGTCGTGGTGAATTACAAAACCTTGCCAGATGGCAAACCGTTGCCTCTCAGCAACGAACAGATGAAGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTc < 3:500011/149‑1 (MQ=255) aaCGTCTGTCAGTCGCGGTCGTGGTGAATTACAAAACCTTGCCAGATGGCAAACCGTTGCCTCTCAGCAACGAACAGATGAAGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTc < 1:277023/149‑1 (MQ=255) aCGTCTGTCAGTCGCGGTCGTGGTGAATTACAAAACCTTGCCAGATGGCAAACCGTTGCCTCTCAGCAACGAACAGATGAAGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCg > 1:168959/1‑149 (MQ=255) cGTCTGTCAGTCGCGGTCGTGGTGAATTACAAAACCTTGCCAGATGGCAAACCGTTGCCTCTCAGCAACGAACAGATGAAGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGt < 4:194671/149‑1 (MQ=255) gTCTGTCAGTCGCGGTCGTGGTGAATTACAAAACCTTGCCAGa < 1:798795/43‑1 (MQ=255) gTCTGTCAGTCGCGGTCGTGGTGAATTACAAAACCTTGCCAGa > 2:798795/1‑43 (MQ=255) tCTGTCAGTCGCGGTCGTGGTGAATTACAAAACCTTGCCAGATGGCAAACCGTTGCCTCTCAGCAACGAACAGATGAAGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAAt < 1:328501/142‑1 (MQ=255) tCTGTCAGTCGCGGTCGTGGTGAATTACAAAACCTTGCCAGATGGCAAACCGTTGCCTCTCAGCAACGAACAGATGAAGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAAt > 2:328501/1‑142 (MQ=255) gTCGCGGTCGTGGTGAATTACAAAACCTTGCCAGATGGCAAACCGTTGCCTCTCAGCAACGAACAGATGAAGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGcc < 1:7010/149‑1 (MQ=255) tCGTGGTGAATTACAAAACCTTGCCAGATGGCAAACCGTTGCCTCTCAGCAACGAACAGATGAAGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGTCTCGCTCAATGTCGTTAACTCGCCGTTCAAt < 1:81238/149‑1 (MQ=255) tCGTGGTGAATTACAAAACCTTGCCAGATGGCAAACCGTTGCCTCTCAGCAACGAACAGATGAAGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAAt < 2:866845/149‑1 (MQ=255) ggtgAATTACAAAACCTTGCCAGATGGCAAACCGTTGCCTCTCAGCAACGAACAGATGAAGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCa > 3:232838/1‑149 (MQ=255) ggtgAATTACAAAACCTTGCCAGATGGCAAACCGTTGCCTCTCAGCAACGAACAGATGAAGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCa < 3:496497/149‑1 (MQ=255) ttACAAAACCTTGCCAGATGGCAAACCGTTGCCTCTCAGCAACGAACAGATGAAGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCAGTGACg > 1:484341/1‑149 (MQ=255) tACAAAACCTTGCCAGATGGCAAACCGTTGCCTCTCAGCAACGAACAGATGAAGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCAGTGACGa < 2:779582/149‑1 (MQ=255) aCAAAACCTTGCCAGATGGCAAACCGTTGCCTCTCAGCAACGAACAGATGAAGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTc < 1:50349/134‑1 (MQ=255) aCAAAACCTTGCCAGATGGCAAACCGTTGCCTCTCAGCAACGAACAGATGAAGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTc > 2:50349/1‑134 (MQ=255) aCAAAACCTTGCCAGATGGCAAACCGTTGCCTCTCAGCAACGAACAGATGAAGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCAGTGACGaa > 1:921899/1‑149 (MQ=255) aaaaCCTTGCCAGATGGCAAACCGTTGCCTCTCAGCAACGAACAGATGAAGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCAGTGACGAAAg > 4:227795/1‑149 (MQ=255) aaaCCTTGCCAGATGGCAAACCGTTGCCTCTCAGCAACGAACAGATGAAGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCAGTGACGAAAgc > 4:135123/1‑149 (MQ=255) aaaCCTTGCCAGATGGCAAACCGTTGCCTCTCAGCAACGAACAGATGAAGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCAGTGACGAAAgc < 2:125991/149‑1 (MQ=255) ggtttGCCATCTGGCAAACCGTTGCCTCTCAGCAACGAACAGATGAAGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTc < 4:375092/83‑1 (MQ=255) ggtttGCCATCTGGCAAACCGTTGCCTCTCAGCAACGAACAGATGAAGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTc > 3:375092/4‑86 (MQ=255) gtttGCCATCTGGCAAACCGTTGCCTCTCAGCAACGAACAGATGAAGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCAGTGACGAAAgcggc > 4:369253/3‑149 (MQ=255) ccTTGCCAGATGGCAAACCGTTGCCTCTCAGCAACGAACAGATGAAGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCAGTGACGAAAgcggc < 2:415304/149‑1 (MQ=255) cTTGCCAGATGGCAAACCGTTGCCTCTCAGCAACGAACAGATGAAGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCAGTGACGaaa < 4:392958/143‑1 (MQ=255) cTTGCCAGATGGCAAACCGTTGCCTCTCAGCAACGAACAGATGAAGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCAGTGACGaaa > 3:392958/1‑143 (MQ=255) cTTGCCAGATGGCAAACCGTTGCCTCTCAGCAACGAACAGATGAAGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCAGTGACGAAAgcggcg > 2:193886/1‑149 (MQ=255) aGATGGCAAACCGTTGCCTCTCAGCAACGAACAGATGAAGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACt < 2:127287/95‑1 (MQ=255) aGATGGCAAACCGTTGCCTCTCAGCAACGAACAGATGAAGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACt > 1:127287/1‑95 (MQ=255) aGATGGCAAACCGTTGCCTCTCAGCAACGAACAGATGAAGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCt < 3:170604/99‑1 (MQ=255) aGATGGCAAACCGTTGCCTCTCAGCAACGAACAGATGAAGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCt > 4:170604/1‑99 (MQ=255) | ACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGTCATACCAAAATGAACGTGGGCGATGTGCAACGTCTGTCAGTCGCGGTCGTGGTGAATTACAAAACCTTGCCAGATGGCAAACCGTTGCCTCTCAGCAACGAACAGATGAAGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCAGTGACGAAAGCGGCG > NC_000913/2014249‑2014537 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |