Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,020,631 C→A 17.3% S182* (TCG→TAG)  fliM → flagellar motor switching and energizing component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,020,6310CA17.3% 322.9 / 48.0 111S182* (TCG→TAG) fliMflagellar motor switching and energizing component
Reads supporting (aligned to +/- strand):  ref base C (47/44);  new base A (10/9);  total (58/53)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.62e-01

Only 100 of 113 total aligned reads displayed.

GGCGATGGACGCTTCCCGACCAAAGTGGAAGGTCGCGAGTTTACCCATACCGAACAGCGCGTCATCAACCGCATGTTGAAACTGGCGCTTGAAGGCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTACGTGCGTTCGGAAATGCAGGTGAAATTTACCAATATCACCACCTCGCCGAACGACATTGTGGTTAACACGCCGTTCCATGTGGAGATTGGCAACCTGACCGGCGAATTTAATATCTGCCTGCCATTCAGCATGATCGAGCCGCTACGGGAATTGTTG  >  NC_000913/2020483‑2020779
                                                                                                                                                    |                                                                                                                                                    
ggcgATGGACGCTTCCCGACCAAAGTGGAAGGTCGCGAGTTTACCCATACCGAACAGCGCGTCATCAACCGCATGTTGAAACTGGCGCTTGAAGGCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTACGTGCGTTc                                                                                                                                                      <  1:158283/149‑1 (MQ=255)
   gATGGACGCTTCCCGACCAAAGTGGAAGGTCGCGAGTTTACCCATACCGAACAGCGCGTCATCAACCGCATGTTGAAACTGGCGCTTGAAGGCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTACGTGCGTTCGGa                                                                                                                                                   >  3:874289/1‑149 (MQ=255)
       gACGCTTCCCGACCAAAGTGGAAGGTCGCGAGTTTACCCATACCGAACAGCGCGTCATCAACCGCATGTTGAAACTGGCGCTTGAAGGCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTACGTGCGTTCGGAAATg                                                                                                                                               >  1:580631/1‑149 (MQ=255)
       gACGCTTCCCGACCAAAGTGGAAGGTCGCGAGTTTACCCATACCGAACAGCGCGTCATCAACCGCATGTTGAAACTGGCGCTTGAAGGCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTACGTGCGTTCGGAAATg                                                                                                                                               >  3:343064/1‑149 (MQ=255)
        aCGCTTCCCGACCAAAGTGGAAGGTCGCGAGTTTACCCATACCGAACAGCGCGTCATCAACCGCATGTTGAAACTGGCGCTTGAAGGCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTACGTGCGTTCGGAAATGc                                                                                                                                              <  3:756789/149‑1 (MQ=255)
          gCTTCCCGACCAAAGTGGAAGGTCGCGAGTTTACCCATACCGAACAGCGCGTCATCAACCGCATGTTGAAACTGGCGCTTGAAGGCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTACGTGCGTTCGGAAATGCAg                                                                                                                                            >  4:750968/1‑149 (MQ=255)
          gCTTCCCGACCAAAGTGGAAGGTCGCGAGTTTACCCATACCGAACAGCGCGTCATCAACCGCATGTTGAAACTGGCGCTTGAAGGCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTACGTGCGTTCGGAAATGCAg                                                                                                                                            <  4:690028/149‑1 (MQ=255)
            ttCCCGACCAAAGTGGAAGGTCGCGAGTTTACCCATACCGAACAGCGCGTCATCAACCGCATGTTGAAACTGGCGCTTGAAGGCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTACGTGCGTTCGGAAATGCAGGt                                                                                                                                          <  3:699594/149‑1 (MQ=255)
              cccGACCAAAGTGGAAGGTCGCGAGTTTACCCATACCGAACAGCGCGTCATCAACCGCATGTTGAAACTGGCGCTTGAAGGCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTACGTGCGTTAGGAAATGCAGGTGa                                                                                                                                        <  3:482069/149‑1 (MQ=255)
               ccGACCAAAGTGGAAGGTCGCGAGTTTACCCATACCGAACAGCGCGTCATCAACCGCATGTTGAAACTGGCGCTTGAAGGCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTACGTGCGTTCGGAAATGCAGGTGaa                                                                                                                                       >  2:348659/1‑149 (MQ=255)
               ccGACCAAAGTGGAAGGTCGCGAGTTTACCCATACCGAACAGCGCGTCATCAACCGCATGTTGAAACTGGCGCTTGAAGGCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTACGTGCGTTCGGAAATGCAGGTGaa                                                                                                                                       >  4:633601/1‑149 (MQ=255)
               ccGACCAAAGTGGAAGGTCGCGAGTTTACCCATACCGAACAGCGCGTCATCAACCGCATGTTGAAACTGGCGCTTGAAGGCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTACGTGCGTTCGGAAATGCAGGTGaa                                                                                                                                       >  2:120739/1‑149 (MQ=255)
                 gACCAAAGTGGAAGGTCGCGAGTTTACCCATACCGAACAGCGCGTCATCAACCGCATGTTGAAACTGGCGCTTGAAGGCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTACGTGCGTTCGGAAATGCAGGTGAAAt                                                                                                                                     >  3:854839/1‑149 (MQ=255)
                 gACCAAAGTGGAAGGTCGCGAGTTTACCCATACCGAACAGCGCGTCATCAACCGCATGTTGAAACTGGCGCTTGAAGGCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTACGTGCGTTCGGAAATGCAGGTGAAAt                                                                                                                                     >  2:932073/1‑149 (MQ=255)
                 gACCAAAGTGGAAGGTCGCGAGTTTACCCATACCGAACAGCGCGTCATCAACCGCATGTTGAAACTGGCGCTTGAAGGCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTACGTGCGTTCGGAAATGCAGGTGAAAt                                                                                                                                     <  1:686788/149‑1 (MQ=255)
                    cAAAGTGGAAGGTCGCGAGTTTACCCATACCGAACAGCGCGTCATCAACCGCATGTTGAAACTGGCGCTTGAAGGCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTACGTGCGTTCGGAAATGCAGGTGAAATTTa                                                                                                                                  <  1:239371/149‑1 (MQ=255)
                    cAAAGTGGAAGGTCGCGAGTTTACCCATACCGAACAGCGCGTCATCAACCGCATGTTGAAACTGGCGCTTGAAGGCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTACGTGCGTTCGGAAATGCAGGTGAAATTTa                                                                                                                                  <  1:655460/149‑1 (MQ=255)
                     aaaGTGGAAGGTCGCGAGTTTACCCATACCGAACAGCGCGTCATCAACCGCATGTTGAAACTGGCGCTTGAAGGCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTACGTGCGTTCGGAAATGCAGGTGAAATTTAc                                                                                                                                 <  4:717709/149‑1 (MQ=255)
                     aaaGTGGAAGGTCGCGAGTTTACCCATACCGAACAGCGCGTCATCAACCGCATGTTGAAACTGGCGCTTGAAGGCTATAGCGACGCCTGGAAGGCGATGAATCCGCTGGAAGTTGAGTACGTGCGTTCGGAAATGCAGGTGAAATTTAc                                                                                                                                 >  4:569298/1‑149 (MQ=255)
                             aGGTCGCGAGTTTACCCATACCGAACAGCGCGTCATCAACCGCATGTTGAAACTGGCGCTTGAAGGCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTACGTGCGTTAGGAAATGCAGGTGAAATTTACCAATATca                                                                                                                         <  4:466248/149‑1 (MQ=255)
                               gTCGCGAGTTTACCCATACCGAACAGCGCGTCATCAACCGCATGTTGAAACTGGCGCTTGAAGGCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTACGTGCGTTCGGAAATGCAGGTGAAATTTACCAATATcacc                                                                                                                       <  3:569298/149‑1 (MQ=255)
                               gTCGCGAGTTTACCCATACCGAACAGCGCGTCATCAACCGCATGTTGAAACTGGCGCTTGAAGGCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTACGTGCGTTCGGAAATGCAGGTGAAATTTACCAATATcacc                                                                                                                       >  1:93757/1‑149 (MQ=255)
                                tCGCGAGTTTACCCATACCGAACAGCGCGTCATCAACCGCATGTTGAAACTGGCGCTTGAAGGCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTACGTGCGTTCGGAAATGCAGGTGAAATTTACCAATATcacca                                                                                                                      >  4:81466/1‑149 (MQ=255)
                                  gcgAGTTTACCCATACCGAACAGCGCGTCATCAACCGCATGTTGAAACTGGCGCTTGAAGGCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTACGTGCGTTCGGAAATGCAGGTGAAATTTa                                                                                                                                  <  4:649271/135‑1 (MQ=255)
                                  gcgAGTTTACCCATACCGAACAGCGCGTCATCAACCGCATGTTGAAACTGGCGCTTGAAGGCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTACGTGCGTTCGGAAATGCAGGTGAAATTTa                                                                                                                                  >  3:649271/1‑135 (MQ=255)
                                  gcgAGTTTACCCATACCGAACAGCGCGTCATCAACCGCATGTTGAAACTGGCGCTTGAAGGCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTACGTGCGTTAGGAAATGCAGGTGAAATTTACCAatat                                                                                                                           <  1:781169/142‑1 (MQ=255)
                                  gcgAGTTTACCCATACCGAACAGCGCGTCATCAACCGCATGTTGAAACTGGCGCTTGAAGGCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTACGTGCGTTAGGAAATGCAGGTGAAATTTACCAatat                                                                                                                           >  2:781169/1‑142 (MQ=255)
                                    gAGTTTACCCATACCGAACAGCGCGTCATCAACCGCATGTTGAAACTGGCGCTTGAAGGCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTACGTGCGTTCGGAAATGCAGGTGAAATTTACCAATATCACCACCTc                                                                                                                  <  2:656150/149‑1 (MQ=255)
                                            ccATACCGAACAGCGCGTCATCAACCGCATGTTGAAACTGGCGCTTGAAGGCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTACGTGCGTTCGGAATTGCAGGTGAAATTTACCAATATCACCACCTCGCCGAACg                                                                                                          >  3:607997/1‑149 (MQ=255)
                                            ccATACCGAACAGCGCGTCATCAACCGCATGTTGAAACTGGCGCTTGAAGGCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTACGTGCGTTCGGAAATGCAGGTGAAATTTACCAATATCACCACCTCGCCGAACg                                                                                                          >  2:686208/1‑149 (MQ=255)
                                              aTACCGAACAGCGCGTCATCAACCGCATGTTGAAACTGGCGGTTGAAGGCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTACGTGCGTTCGGAAATGCAGGTGAAATTTACCAATATc                                                                                                                          <  1:423512/131‑1 (MQ=255)
                                              aTACCGAACAGCGCGTCATCAACCGCATGTTGAAACTGGCGCTTGAAGGCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTACGTGCGTTCGGAAATGCAGGTGAAATTTACCAATATc                                                                                                                          >  2:423512/1‑131 (MQ=255)
                                              aTACCGAACAGCGCGTCATCAACCGCATGTTGAAACTGGCGCTTGAAGGCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTACGTGCGTTAGGAAATGCAGGTGAAATTTAcc                                                                                                                                <  4:195138/125‑1 (MQ=255)
                                              aTACCGAACAGCGCGTCATCAACCGCATGTTGAAACTGGCGCTTGAAGGCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTACGTGCGTTAGGAAATGCAGGTGAAATTTAcc                                                                                                                                >  3:195138/1‑125 (MQ=255)
                                               tACCGAACAGCGCGTCATCAACCGCATGTTGAAACTGGCGCTTGAAGGCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTACGTGCGTTCGGAAATGCAGGTGAAATTTACCAATATCACCACCTCGCCGAACGACa                                                                                                       >  2:377936/1‑149 (MQ=255)
                                                    aaCAGCGCGTCATCAACTGCATGTTGAAACTGGCGCTTGAAGGCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTACGTGCGTTCGGAAATGCAGGTGAACTTTACCAATATCACCACCTCGCCGAACGACATtgtg                                                                                                  <  4:577827/149‑1 (MQ=255)
                                                       aGCGCGTCATCCACCGCATGTTGAAACTGGCGCTTGAAGGCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTACGTGCGTTAGGAAATGCAGGTGAACTTTACCAATATCACTACCTCGCCGAACGACATTGTGGtt                                                                                               >  3:608903/1‑149 (MQ=255)
                                                       aGCGCGTCATCAACCGCATGTTGAAACTGGCGCTTGAAGGCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTACGTGCGTTAGGAAATGCAGGTGAAATTTACCAATATCACCACCTCGCCGAACGACATTGTGGtt                                                                                               >  2:920371/1‑149 (MQ=255)
                                                         cgcgTCATCAACCGCATGTTGAAACTGGCGCTTGAAGGCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTACGTGCGTTCGGAAATGCAGGTGAAATTTACCAATATCACCACCTCGCCGAACGACATTGTGGTTac                                                                                             <  4:327268/149‑2 (MQ=255)
                                                           cgTCATCAACCGCATGTTGAAACTGGCGCTTGAAGGCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTACGTGCGTTCGGAAATGCAGGTGAAATTTACCAATATCACCACCTCGCCGAACGACATTGTGGTTAaca                                                                                           <  1:241778/149‑1 (MQ=255)
                                                            gTCATCAACCGCATGTTGAAACTGGCGCTTGAAGGCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTACGTGCGTTAGGAAATGCAGGTGAAATTTAc                                                                                                                                 <  2:340784/110‑1 (MQ=255)
                                                              catcaACCGCATGTTGAAACTGGCGCTTGAAGGCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTACGTGCGTTCGGAAATGCAGGTGAAATTTACCAATATCACCACCTCGCCGAACGACATTGTGGTTAACACGc                                                                                        >  2:722637/1‑149 (MQ=255)
                                                               atcaACCGCATGTTGAAACTGGCGCTTGAAGGCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTACGTGCGTTCGGAAATGCAGGTGAAATTTACCAATATCACCACCTCGCCGAACGACATTGTGGTTAACACGcc                                                                                       >  1:195543/1‑149 (MQ=255)
                                                               atcaACCGCATGTTGAAACTGGCGCTTGAAGGCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTACGTGCGTTCGGAAATGCAGGTGAAATTTACCAATATCACCACCTCGCCGAACGACATTGTGGTTAACACGcc                                                                                       >  4:889905/1‑149 (MQ=255)
                                                                  aaCCGCTTGTTGAAACTGGCGCTTGAAGGCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTACGTGCGTTAGGAAATGCAGGTGAAATTTACCAATATCACCACCTCGCCGAACGACATTGTGGTTAAAACGCCGtt                                                                                    >  2:450752/1‑149 (MQ=255)
                                                                  aaCCGCATGTTGAAACTGGCGCTTGAAGGCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTACGTGCGTTCGGAAATGCAGGTGAAATTTACCAATATCACCACCTCGCCGAACGACATTGTGGTTAACACGc                                                                                        >  2:913104/1‑145 (MQ=255)
                                                                  aaCCGCATGTTGAAACTGGCGCTTGAAGGCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTACGTGCGTTCGGAAATGCAGGTGAAATTTACCAATATCACCACCTCGCCGAACGACATTGTGGTTAACACGc                                                                                        <  1:913104/145‑1 (MQ=255)
                                                                  aaCCGCATGTTGAAACTGGCGCTTGAAGGCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTACGTGCGTTCGGAAATGCAGGTGAAATTTACCAATATCACCACCTCGCCGAACGACATTGTGGTTAACACGCCGtt                                                                                    >  2:472021/1‑149 (MQ=255)
                                                                  aaCCGCATGTTGAAACTGGCGCTTGAAGGCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTACGTGCGTTAGGAAATGCAGGTGAAATTTACCAATATCACCACCTCGCCGAACGACATTGTGGTTAACACGCCGtt                                                                                    >  3:239146/1‑149 (MQ=255)
                                                                   aCCGCATGTTGAAACTGGCGCTTGAAGGCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTACGTGCGTTCGGAAATGCAGGTGAAATTTACCAATATCACCACCTCGCCGAACGACATTGTGGTTAACACGCCGTTc                                                                                   <  3:143897/149‑1 (MQ=255)
                                                                      gCATGTTGAAACTGGCGCTTGAAGGCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTACGTGCGTTCGGAAATGCAGGTGAAATTTACCAATATCACCACCTCGCCGAACGACATTGTGGTTAACACGCCGTTCCAt                                                                                <  1:348659/149‑1 (MQ=255)
                                                                      gCATGTTGAAACTGGCGCTTGAAGGCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTACGTGCGTTCGGAAATGCAGGTGAAATTTACCAATATCACCACCTCGCCGAACGACATTGTGGTTAACACGCCGTTCCAt                                                                                <  3:371129/149‑1 (MQ=255)
                                                                      gCATGTTGAAACTGGCGCTTGAAGGCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTACGTGCGTTCGGAAATGCAGGTGAAATTTACCAATATCACCACCTCGCCGAACGACATTGTGGTTAACACGCCGTTCCAt                                                                                <  4:223401/149‑1 (MQ=255)
                                                                         tGTTGAAACTGGCGCTTGAAGGCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTACGTGCGTTCGGAAATGCAGGTGAAATTTACCAATATCACCACCTCGCCGAACGACATTGTGGTTAACACGCCGt                                                                                     <  1:100836/141‑1 (MQ=255)
                                                                         tGTTGAAACTGGCGCTTGAAGGCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTACGTGCGTTCGGAAATGCAGGTGAAATTTACCAATATCACCACCTCGCCGAACGACATTGTGGTTAACACGCCGt                                                                                     >  2:100836/1‑141 (MQ=255)
                                                                         tGTTGAAACTGGCGCTTGAAGGCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTACGTGCGTTCGGAAATGCAGGTGAAATTTACCAATATCACCACCTCGCCGAACGACATTGTGGTTAACACGCCGTTCCAtgtg                                                                             <  3:633601/149‑1 (MQ=255)
                                                                           ttGAAACTGGCGCTTGAAGGCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTACGTGCGTTAGGAAATGCAGGTGAAATTTACCAATATCACCACCTCGCCGAACGACATTGTGGTTAACACGCCGTTCCATGTGga                                                                           >  4:30484/1‑149 (MQ=255)
                                                                              aaaCTGGCGCTTGAAGGCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTACGTGCGTTCGGAAATGCAGGTGAAATTTACCAATATCACCACCTCGCCGAACGACATTGTGGTTAACACGCCGTTCCATGTGGAGAt                                                                        >  3:182434/1‑149 (MQ=255)
                                                                                aCTGGCGCTTGAAGGCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTGGAGTACGTGCGTTCGGAAATGCAGGTGAAATTAACCAATATCACCCCCTCGCCGAACGACATTGTGGTTAACACGCCGTTCCATGTGGAGATTg                                                                      <  4:881432/149‑1 (MQ=255)
                                                                                 cTGGCGCTTGAAGGCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTACGTGCGTTCGGAAATGCAGGTGAAATTTACCAATATCACCACCTCGCCGAACGACATTGTGGTTAACACGCCGTTCCATGTGGAGATTgg                                                                     >  1:151356/1‑149 (MQ=255)
                                                                                   ggCGCTTGAAGGCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTACGTGCGTTCGGAAATGCAGGTGAAATTTACCAATATCACCACCTCGCCGAACGACATTGTGGTTAACACGCCGTTCCATGTGGAGATTGGCa                                                                   >  3:614571/1‑149 (MQ=255)
                                                                                   ggCGCTTGAAGGCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTACGTGCGTTCGGAAATGCAGGTGAAATTTACCAATATCACCACCTCGCCGAACGACATTGTGGTTAACACGCCGTTCCATGTGGAGATTGGCa                                                                   >  2:908505/1‑149 (MQ=255)
                                                                                   ggCGCTTGAAGGCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTACGTGCGTTCGGAAATGCAGGTGAAATTTACCAATATCACCACCTCGCCGAACGACATTGTGGTTAACACGCCGTTCCATGTGGAGATTGGCa                                                                   >  1:787289/1‑149 (MQ=255)
                                                                                     cgcTTGAAGGCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTACGTGCGTTCGGAAATGCAGGTGAAATTTACCAATATCACCACCTCGCCGAACGACATTGTGGTTAACACGCCGTTCCATGTGGAGATTGGCAAc                                                                 <  1:632081/149‑1 (MQ=255)
                                                                                       cTTGAAGGCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTACGTGCGTTAGGAAATGCAGGTGAAATTTACCAATATCACCACCTCGCCGAACGACATTGTGGTTAACACGCCGTTCCATGTGGAGATTGGCAACCt                                                               <  3:30484/149‑1 (MQ=255)
                                                                                          gAAGGCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTACGTGCGTTCGGAAATGCAGGTGAAATTTACCAATATCACCACCTCGCCGAACGACATTGTGGTTAACACGCCGTTCCATGTGGAGATTGGCAACCTGAc                                                            <  1:472021/149‑1 (MQ=255)
                                                                                          gAAGGCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTACGTGCGTTCGGAAATGCAGGTGAAATTTACCAATATCACCACCTCGCCGAACGACATTGTGGTTAACACGCCGTTCCATGTGGAGATTGGCAACCTGAc                                                            <  1:932073/149‑1 (MQ=255)
                                                                                            aGGCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTACGTGCGTTCGGAAATGCAGGTGAAATTTACCAATATCACCACCTCGCCGAACGACATTGTGGTTAACACGCCGTTCCATGTGGAGATTGGCAACCTGACCg                                                          >  3:908942/1‑149 (MQ=255)
                                                                                            aGGCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTACGTGCGTTCGGAAATGCAGGTGAAATTTACCAATATCACCACCTCGCCGAACGACATTGTGGTTAACACGCCGTTCCATGTGGAGATTGGCAACCTGACCg                                                          >  1:712134/1‑149 (MQ=255)
                                                                                              gCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTACGTGCGTTCGGAAATGCTGGTGAAATTTAc                                                                                                                                 <  2:825617/76‑1 (MQ=255)
                                                                                               cTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTACGTGCGTTCGGAAATGCAg                                                                                                                                            >  2:304690/1‑64 (MQ=255)
                                                                                               cTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTACGTGCGTTCGGAAATGCAg                                                                                                                                            <  1:304690/64‑1 (MQ=255)
                                                                                                    gCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTACGTGCGTTCGGAAATGCAGGTGAAATTTACCAATATCACCACCTCGCCGAACGACATTGTGGTTAACACGCCGTTCCATGTGGAGATTGGCAACCTGACCGGCGAAttt                                                  <  1:908505/149‑1 (MQ=255)
                                                                                                        cGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTACGTGCGTTCGGAAATGCAGGTGAAATTTACCAATATCACCACCTCGCCGAACGACATTGTGGTTAACACGCCGTTCCATGTGGAGATTGGCAACCTGACCGGCGAATTTAata                                              <  1:377936/149‑1 (MQ=255)
                                                                                                        cGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTACGTGCGTTCGGAAATGCAGGTGAAATTTACCAATATCACCACCTCGCCGAACGACATTGTGGTTAACACGCCGTTCCATGTGGAGATTGGCAACCTGACCGGCGAATTTAata                                              <  2:582342/149‑1 (MQ=255)
                                                                                                         gCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTACGTGCGTTCGGAAATGCAGGTGAAATTTACCAATATCACCACCTCGCCGAACGACATTGTGGTTAACACGCCGTTCCATGTGGAGATTGGCAACCTGACCGGCGAATTTaa                                                <  1:394311/146‑1 (MQ=255)
                                                                                                         gCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTACGTGCGTTCGGAAATGCAGGTGAAATTTACCAATATCACCACCTCGCCGAACGACATTGTGGTTAACACGCCGTTCCATGTGGAGATTGGCAACCTGACCGGCGAATTTaa                                                >  2:394311/1‑146 (MQ=255)
                                                                                                         gCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTACGTGCGTTCGGAAATGCAGGTGAAATTTACCAATATCACCACCTCGCCGAACGACATTGTGGTTAACACGCCGTTCCATGTGGAGATTGGCAACCTGACCGGCGAATTTAatat                                             <  1:752105/149‑1 (MQ=255)
                                                                                                                  gCGATTAATCCGCTGGAAGTTGAGTACGTGCGTTCGGAAATGCAGGTGAAATTTACCAATATCACCACCTCGCCGAACGACATTGTGGTTAACACGCCGTTCCATGTGGAGATTGGCAACCTGACCGGCGAATTTAATATCtgcctgcc                                    <  4:636778/149‑1 (MQ=255)
                                                                                                                    gATTAATCCGCTGGAAGTTGAGTACGTGCGTTCGGAAATGCAGGTGAAATTTACCAATATCACCACCTCGCCGAACGACATTGTGGTTAACACGCCGTTCCATGTGGAGATTGGCAACCTGACCGGCGAATTTAATATCTGCCTGCCAt                                  >  4:234810/1‑149 (MQ=255)
                                                                                                                          tCCGCTGGAAGTTGAGTACGTGCGTTCGGAAATGCAGGTGAAATTTACCAATATCACCACCTCGCCGAACGACATTGTGGTTAACACGCCGTTCCATGTGGAGATTGGCAACCTGACCGGCGAATTTAATATCTGCCTGCCATTCAGCa                            <  1:120739/149‑1 (MQ=255)
                                                                                                                            cGCTGGAAGTTGAGTACGTGCGTTAGGAAATGCAGGTGAAATTTACCAATATCACCACCTCGCCGAACGACATTGTGGTTAACACGCCGTTCCATGTGGAGATTGGCAACCTGACCGGCGAATTTAATATCTGCCTGCCATTCAGCATg                          <  1:450752/149‑1 (MQ=255)
                                                                                                                              cTGGAAGTTGAGTACGTGCGTTCGGAAATGCAGGTGAAATTTACCAATATCACCACCTCGCCGAACGACATTGTGGTTAACACGCCGTTCCATGTGGAGATTGGCAACCTGACCGGCGAATTTAATATCTGCCTGCCATTCAGCATGAt                        <  2:580631/149‑1 (MQ=255)
                                                                                                                              cTGGAAGTTGAGTACGTGCGTTCGGAAATGCAGGTGAAATTTACCAATATCACCACCTCGCCGAACGACATTGTGGTTAACACGCCGTTCCATGTGGAGATTGGCAACCTGACCGGCGAATTTAATATCTGCCTGCCATTCAGCATGAt                        <  4:343064/149‑1 (MQ=255)
                                                                                                                                ggAAGTTGAGTACCTGCGTTCGGAAATGCAGGTGAAATTTACCAATATCACCACCTCGCCGAACGACATTGTGGTTCCCACGCCGTTCCATGTGCCGATTGGCAACCTGACCGGCGAATTTAATATCTGCCTGCCATTCAGCATGATCg                      >  2:618987/1‑149 (MQ=255)
                                                                                                                                   aGTTGAGTACGTGCGTTAGGAAATGCAGGTGAAATTTACCAATATCACCACCTCGCCGAACGACATTGTGGTTAACACGCCGTTCCATGTGGAGATTGGCAACCTGACCGGCGAATTTAATATCTGCCTGCCATTCAGCATGATCGAGc                   >  2:58471/1‑149 (MQ=255)
                                                                                                                                    gTTGAGTACGTGCGTTCGGAAATGCAGGTGAAATTTACCAATATCACCACCTCGCCGAACGACATTGTGGTTAACACGCCGTTCCATGTGGAGATTGGCAACCTGACCGGCGAATTTAATATCTGCCTGCCATTCAGCATGATCGAGcc                  >  3:626205/1‑149 (MQ=255)
                                                                                                                                    gTTGAGTACGTGCGTTCGGAAATGCAGGTGAAATTTACCAATATCACCACCTCGCCGAACGACATTGTGGTTAACACGCCGTTCCATGTGGAGATTGGCAACCTGACCGGCGAATTTAATATCTGCCTGCCATTCAGCATGATCGAGcc                  <  2:195543/149‑1 (MQ=255)
                                                                                                                                    cttGAGTACGTGCGTTCGGAAATGCAGGTGAAATTTACCAATACCACCACCTCGCCGAACGACATTGTGGTTAACACGCCGTTCCATGTGGAGATTGGCAACCTGACCGGCGAATTTAATATCTGCCTGCCATTCAGCATGATCGAGcc                  <  3:81466/148‑1 (MQ=255)
                                                                                                                                     ttGAGTACGTGCGTTCGGAAATGCAGGTGAAATTTACCAATATCACCACCTCGCCGAACGACATTGTGGTTAACACGCCGTTCCATGTGGAGATTGGCAACCTGACCGGCGAATTTAATATctgc                                         <  3:769591/125‑1 (MQ=255)
                                                                                                                                     ttGAGTACGTGCGTTCGGAAATGCAGGTGAAATTTACCAATATCACCACCTCGCCGAACGACATTGTGGTTAACACGCCGTTCCATGTGGAGATTGGCAACCTGACCGGCGAATTTAATATctgc                                         >  4:769591/1‑125 (MQ=255)
                                                                                                                                      tGAGTACGTGCGTTCGGAAATGCAGGTGAAATTTACCAATATCACCACCTCGCCGAACGACATTGTGGTTAACACGCCGTTCCATGTGGAGATTGGCAACCTGACCGGCGAATTTAATATCTGCCTGCCATTCAGCATGATCGAGCCGc                >  1:581869/1‑149 (MQ=255)
                                                                                                                                         gTACGTGCGTTCGGAAATGCAGGTGAAATTTACCAATATCACCACCTCGCCGAACGACATTGTGGTTAACACGCCGTTCCATGTGGAGATTGGCAACCTGACCGGCGAATTTAATATCTGCCTGCCATTCAGCATGATCGAGCCGCTAc             >  4:481746/1‑149 (MQ=255)
                                                                                                                                         gTACGTGCGTTCGGAAATGCAGGTGAAATTTACCAATATCACCACCTCGCCGAACGACATTGTGGTTAACACGCCGTTCCATGTGGAGATTGGCAACCTGACCGGCGAATTTAATATCTGCCTGCCATTCAGCATGATCGAGCCGCTAc             >  2:923993/1‑149 (MQ=255)
                                                                                                                                           aCGTGCGTTCGGAAATGCAGGTGAAATTTACCAATATCACCACCTCGCCGAACGACATTGTGGTTAACACGCCGTTCCATGTGGAGATTGGCAACCTGACCGGCGAATTTAATATCTGCCTGCCATTCAGCATGATCGAGCCGCTACgg           <  1:686208/149‑1 (MQ=255)
                                                                                                                                           aCGTGCGTTAGGAAATGCAGGTGAAATTTACCAATATCACCACCTCGCCGAACGACATTGTGGTTAACACGCCGTTCCATGTGGAGATTGGCAACCTGACCGGCGAATTTAATATCTGCCTGCCATTCAGCATGATCGAGCCGCTACgg           <  2:646048/149‑1 (MQ=255)
                                                                                                                                                   tCGGAAATGCAGGTGAAATTTACCAATATCACCACCTCGCCGAACGACATTGTGGTTAACACGCCGTTCCATGTGGAGATTGGCAACCTGACCGGCGAATTTAATATCTGCCTGCCATTCAGCATGATCGAGCCGCTACGGGAAttgtt   <  4:626205/149‑1 (MQ=255)
                                                                                                                                                   tCGGAAATGCAGGTGAAATTTACCAATATCACCACCTCGCCGAACGACATTGTGGTTAACACGCCGTTCCATGTGGAGATTGGCAACCTGACCGGCGAATTTAATATCTGCCTGCCATTCAGCATGATCGAGCCGCTACGGGAAttgtt   <  3:750968/149‑1 (MQ=255)
                                                                                                                                                   tCGGAAATGCAGGTGAAATTTACCAATATCACCACCTCGCCGAACGACATTGTGGTTAACACGCCGTTCCATGTGGAGATTGGCAACCTGACCGGCGAATTTAATATCTGCCTGCCATTCAGCATGATCGAGCCGCTACGGGAAttgtt   <  3:250988/149‑1 (MQ=255)
                                                                                                                                                    cGGAAATGCAGGTGAAATTTACCAATATCACCACCTCGCCGAACGACATTGTGGTTAACACGCCTTTCCCTGTGCAGATTGGCAACCAGACCGGCCAATTTAATATCTGCCTGCCATTCAGCATGATCGAGCCGCTACAGGAAttgttg  <  4:295199/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GGCGATGGACGCTTCCCGACCAAAGTGGAAGGTCGCGAGTTTACCCATACCGAACAGCGCGTCATCAACCGCATGTTGAAACTGGCGCTTGAAGGCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTACGTGCGTTCGGAAATGCAGGTGAAATTTACCAATATCACCACCTCGCCGAACGACATTGTGGTTAACACGCCGTTCCATGTGGAGATTGGCAACCTGACCGGCGAATTTAATATCTGCCTGCCATTCAGCATGATCGAGCCGCTACGGGAATTGTTG  >  NC_000913/2020483‑2020779

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: