Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,025,834 C→A 67.7% R108R (CGC→CGA yedP → putative mannosyl‑3‑phosphoglycerate phosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,025,8340CA67.7% 113.6 / 110.8 99R108R (CGC→CGAyedPputative mannosyl‑3‑phosphoglycerate phosphatase
Reads supporting (aligned to +/- strand):  ref base C (17/15);  new base A (32/35);  total (49/50)
Fisher's exact test for biased strand distribution p-value = 6.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.26e-01

Only 100 of 107 total aligned reads displayed.

CGTTGGGGCTACAAGGTTTACCGCTGATTGCCGAAAATGGCGCAGTGATCCAGCTTGCTGAGCAATGGCAGGAGATAGACGGTTTTCCACGCATCATCTCAGGTATTAGCCATGGCGAAATCAGCCTGGTTTTAAATACGCTACGCGAGAAAGAACATTTTAAATTCACGACTTTTGATGATGTCGACGATGCAACCATCGCCGAATGGACGGGATTAAGCCGTAGCCAGGCGGCGCTGACGCAGCTTCATGAGGCGTCGGTAACGCTAATCTGGCGCGACAGTGACGAGC  >  NC_000913/2025689‑2025979
                                                                                                                                                 |                                                                                                                                                 
cGTTGGGGCTACAAGGTTTACCGCTGATTGCCGAAAATGGCGCAGTGATCCAGCTTGCTGAGCAATGGCAGGAGATAGACGGTTTTCCACGCATCATCTCAGGTATTAGCCATGGCGAAATCAGCCTGGTTTTAAATACGCTACGagag                                                                                                                                                <  4:208747/149‑1 (MQ=255)
cGTTGGGGCTACAAGGTTTACCGCTGATTGCCGAAAATGGCGCAGTGATCCAGCTTGCTGAGCAATGGCAGGAGATAGACGGTTTTCCACGCATCATCTCAGGTATTAGCCATGGCGAAATCAGCCTGGTTTTAAATACGCTACGCgag                                                                                                                                                <  2:925815/149‑1 (MQ=255)
  ttGGGGCTACAAGGTTTACCGCTGATTGCCGAAAATGGCGCAGTGATCCAGCTTGCTGAGCAATGGCAGGAGATAGACGGTTTTCCACGCATCATCTCAGGTATTAGCCATGGCGAAATCAGCCTGGTTTTAAATACGCTACGAGagaa                                                                                                                                              >  4:124635/1‑149 (MQ=255)
     gggCTACAAGGTTTACCGCTGATTGCCGAAAATGGCGCAGTGATCCAGCTTGCTGAGCAATGGCAGGAGATAGACGGTTTTCCACGCATCATCTCAGGTATTAGCCATGGCGAAATCAGCCTGGTTTTAAATACGCTACGagag                                                                                                                                                <  3:415600/144‑1 (MQ=255)
     gggCTACAAGGTTTACCGCTGATTGCCGAAAATGGCGCAGTGATCCAGCTTGCTGAGCAATGGCAGGAGATAGACGGTTTTCCACGCATCATCTCAGGTATTAGCCATGGCGAAATCAGCCTGGTTTTAAATACGCTACGagag                                                                                                                                                >  4:415600/1‑144 (MQ=255)
     gggCTACAAGGTTTACCGCTGATTGCCGAAAATGGCGCAGTGATCCAGCTTGCTGAGCAATGGCAGGAGATAGACGGTTTTCCACGCATCATCTCAGGTATTAGCCATGGCGAAATCAGCCTGGTTTTAAATACGCTACGAGagaaaga                                                                                                                                           >  4:610146/1‑149 (MQ=255)
       gCTTCAAGGTTTACCGCTGATTGCCGAAAATGGCGCAGTGATCCAGCTTGCTGAGCAATGGCAGGAGATAGACGGTTTTCCACGCATCATCTCAGGTATTAGCCATGGCGAAATCAGCCTGGTTTTAAATACGCTACGAGAGAAAGAAc                                                                                                                                         >  3:710975/1‑149 (MQ=255)
       gCTACAAGGTTTACCGCTGATTGCCGAAAATGGCGCAGTGATCCAGCTTGCTGAGCAATGGCAGGAGATAGACGGTTTTCCACGCATCATCTCAGGTATTAGCCATGGCGAAATCAGCCTGGTTTTAAATACGCTACGAGAGAAAGAAc                                                                                                                                         <  3:326037/149‑1 (MQ=255)
        cTACAAGGTTTACCGCTGATTGCCGAAAATGGCGCAGTGATCCAGCTTGCTGAGCAATGGCAGGAGATAGACGGTTTTCCACGCATCATCTCAGGTATTAGCCATGGCGAAATCAGCCTGGTTTTAAATACGCTACGAGAGAAAGAACa                                                                                                                                        >  3:373505/1‑149 (MQ=255)
         tACAAGGTTTACCGCTGATTGCCGAAAATGGCGCAGTGATCCAGCTTGCTGAGCAATGGCAGGAGATAGACGGTTTTCCACGCATCATCTCAGGTATTAGCCATGGCGAAATCAGCCTGGTTTTAAATACGCTACGAGAGAAAGAACAt                                                                                                                                       >  4:10845/1‑149 (MQ=255)
          aCAAGGTTTACCGCTGATTGCCGAAAATGGCGCAGTGATCCAGCTTGCTGAGCAATGGCAGGAGATAGACGGTTTTCCACGCATCATCTCAGGTATTAGCCATGGCGAAATCAGCCTGGTTTTAAATACGCTACGCGAGAAAGAACAtt                                                                                                                                      <  1:324497/149‑1 (MQ=255)
          aCAAGGTTTACCGCTGATTGCCGAAAATGGCGCAGTGATCCAGCTTGCTGAGCAATGGCAGGAGATAGACGGTTTTCCACGCATCATCTCAGGTATTAGCCATGGCGAAATCAGCCTGGTTTTAAATACGCTACGAGAGAAAGAACAtt                                                                                                                                      <  4:1539/149‑1 (MQ=255)
           cAAGGTTTACCGCTGATTGCCGAAAATGGCGCAGTGATCCAGCTTGCTGAGCAATGGCAGGAGATAGACGGTTTTCCACGCATCATCTCAGGTATTAGCCATGGCGAAATCAGCCTGGTTTTAAATACGCTACGCGAGAAAGAACAtt                                                                                                                                      >  1:195859/1‑148 (MQ=255)
           cAAGGTTTACCGCTGATTGCCGAAAATGGCGCAGTGATCCAGCTTGCTGAGCAATGGCAGGAGATAGACGGTTTTCCACGCATCATCTCAGGTATTAGCCATGGCGAAATCAGCCTGGTTTTAAATACGCTACGCGAGAAAGAACAtt                                                                                                                                      <  2:195859/148‑1 (MQ=255)
           cAAGGTTTACCGCTGATTGCCGAAAATGGCGCAGTGATCCAGCTTGCTGAGCAATGGCAGGAGATAGACGGTTTTCCACGCATCATCTCAGGTATTAGCCATGGCGAAATCAGCCTGGTTTTAAATACGCTACGAGAGAAAGAACAttt                                                                                                                                     >  3:676347/1‑149 (MQ=255)
           cAAGGTTTACCGCTGATTGCCGAAAATGGCGCAGTGATCCAGCTTGCTGAGCAATGGCAGGAGATAGACGGTTTTCCACGCATCATCTCAGGTATTAGCCATGGCGAAATCAGCCTGGTTTTAAATACGCTACGAGAGAAAGAACAttt                                                                                                                                     <  4:86358/149‑1 (MQ=255)
               gTTTACCGCTGATTGCCGAAAATGGCGCAGTGATCCAGCTTGCTGAGCAATGGCAGGAGATAGACGGTTTTCCACGCATCATCTCAGGTATTAGCCATGGCGAAATCAGCCTGGTTTTAAATACGCTACGAGAGAAAGAACATTTTaaa                                                                                                                                 >  1:730161/1‑149 (MQ=255)
               gTTTACCGCTGATTGCCGAAAATGGCGCAGTGATCCAGCTTGCTGAGCAATGGCAGGAGATAGACGGTTTTCCACGCATCATCTCAGGTATTAGCCATGGCGAAATCAGCCTGGTTTTAAATACGCTACGAGAGAAAGAACATTTTaaa                                                                                                                                 >  4:791303/1‑149 (MQ=255)
                   aCCGCTGATTGCCGAAAATGGCGCAGTGATCCAGCTTGCTGAGCAATGGCAGGAGATAGACGGTTTTCCACGCATCATCTCAGGTATTAGCCATGGCGAAATCAGCCTGGTTTTAAATACGCTACGAGAGAAAGAACATTTTAAATTCa                                                                                                                             <  2:615975/149‑1 (MQ=255)
                      gCTGATTGCCGAAAATGGCGCAGTGATCCAGCTTGCTGAGCAATGGCAGGAGATAGACGGTTTTCCACGCATCATCTCAGGTATTAGCCATGGCGAAATCAGCCTGGTTTTAAATACGCTACGCGAGAAAGAACATTTTAAATCCACGa                                                                                                                          >  1:519872/1‑149 (MQ=255)
                             gCCGAAAATGGCGCAGTGATCCAGCTTGCTGAGCAATGGCAGGAGATAGACGGTTTTCCACGCATCATCTCAGGTATTAGCCATGGCGAAATCAGCCTGGTTTTAAATACGCTACGAGAGAAAGAACATTTTAAATTCACGACTTTtga                                                                                                                   <  4:694938/149‑1 (MQ=255)
                              ccGAAAATGGCGCAGTGATCCAGCTTGCTGAGCAATGGCAGGAGATAGACGGTTTTCCACGCATCATCTCAGGTATTAGCCATGGCGAAATCAGCCTGGTTTTAAATACGCTACGCGAGAAAGAACATTTTAAATTCACGACTTTtgat                                                                                                                  <  3:494851/149‑1 (MQ=255)
                               cGAAAATGGCGCAGTGATCCAGCTTGCTGAGCAATGGCAGGAGATAGACGGTTTTCCACGCATCATCTCAGGTATTAGCCATGGCGAAATCAGCCTGGTTTTAAATACGCTACGAGAGAAAGAACATTTTAAATTCACGACTTTtgatg                                                                                                                 >  1:799927/1‑149 (MQ=255)
                                gAAAATGGCGCAGTGATCCAGCTTGCTGAGCAATGGCAGGAGATAGACGGTTTTCCACGCATCATCTCAGGTATTAGCCATGGCGAAATCAGCCTGGTTTTAAATACGCTACGAGAGAAAGAACAtttt                                                                                                                                    <  3:641962/129‑1 (MQ=255)
                                gAAAATGGCGCAGTGATCCAGCTTGCTGAGCAATGGCAGGAGATAGACGGTTTTCCACGCATCATCTCAGGTATTAGCCATGGCGAAATCAGCCTGGTTTTAAATACGCTACGAGAGAAAGAACAtttt                                                                                                                                    >  4:641962/1‑129 (MQ=255)
                                 aaaaTGGCGCAGTGATCCAGCTTGCTGAGCAATGGCAGGAGATAGACGGTTTTCCACGCATCATCTCAGGTATTAGCCATGGCGAAATCAGCCTGGTTTTAAATACGCTACGCGAGAAAGAACATTTTAAAtt                                                                                                                               <  4:278600/133‑1 (MQ=255)
                                 aaaaTGGCGCAGTGATCCAGCTTGCTGAGCAATGGCAGGAGATAGACGGTTTTCCACGCATCATCTCAGGTATTAGCCATGGCGAAATCAGCCTGGTTTTAAATACGCTACGCGAGAAAGAACATTTTAAAtt                                                                                                                               >  3:278600/1‑133 (MQ=255)
                                 aaaaTGGCGCAGTGATCCAGCTTGCTGAGCAATGGCAGGAGATAGACGGTTTTCCACGCATCATCTCAGGTATTAGCCATGGCGAAATCAGCCTGGTTTTAAATACGCTACGCGAGAAAGAACATTTTAAATTCACGACTTTTgatgat                                                                                                               <  4:896950/149‑1 (MQ=255)
                                 aaaaTGGCGCAGTGATCCAGCTTGCTGAGCAATGGCAGGAGATAGACGGTTTTCCACGCATCATCTCAGGTATTAGCCATGGCGAAATCAGCCTGGTTTTAAATACGCTACGAGAGAAAGAACATTTTAAATTCACGACTTTTgatgat                                                                                                               <  3:610146/149‑1 (MQ=255)
                                 aaaaTGGCGCAGTGATCCAGCTTGCTGAGCAATGGCAGGAGATAGACGGTTTTCCACGCATCATCTCAGGTATTAGCCATGGCGAAATCAGCCTGGTATTAAATACGCTACGAGAGAAAGAACATTTTAAATTCACGACTTTTgatgat                                                                                                               <  2:799927/149‑1 (MQ=255)
                                         gcAGTGATCCAGCTTGCTGAGCAATGGCAGGAGATAGACGGTTTTCCACGCATCATCTCAGGTATTAGCCATGGCGAAATCAGCCTGGTTTTAAATACGCTACGAGAGAAAGAACATTTTAAATTCACGACTTTTGATGATGTcgacga                                                                                                       <  3:10845/149‑1 (MQ=255)
                                                tCCAGCTTGCTGAGCAATGGCAGGAGATAGACGGTTTTCCACGCATCATCTCAGGTATTAGCCATGGCGAAATCAGCCTGGTTTTAAATACGCTACGAGAGAAAGAACATTTTAAATTCACGACTTTTGATGATGTCGACGATGCAAcc                                                                                                >  1:613512/1‑149 (MQ=255)
                                                 ccAGCTTGCTGAGCAATGGCAGGAGATAGACGGTTTTCCACGCATCATCTCAGGTATTAGCCATGGCGAAATCAGCCTGGTTTTAAATACGCTACGAGAGAAAGAACATTTTAAATTCACGACTTTTGATGATGTCGACGATGCAACCa                                                                                               >  3:746585/1‑149 (MQ=255)
                                                   aGCTTGCTGAGCAATGGCAGGAGATAGACGGTTTTCCACGCATCATCTCAGGTATTAGCCATGGCGAAATCAGCCTGGTTTTAAATACGCTACGCGAGAAAGAACATTTTAAATTCACGACTTTTGATGATGTCGACGATGCAACCATc                                                                                             >  1:503440/1‑149 (MQ=255)
                                                    gCTTGCTGAGCAATGGCAGGAGATAGACGGTTTTCCACGCATCATCTCAGGTATTAGCCATGGCGAAATCAGCCTGGTTTTAAATACGCTACGAGAGAAAGAACATTTTAAATTCACGACTTTTGATGATGTCGACGATGCAACCATCg                                                                                            >  1:339729/1‑149 (MQ=255)
                                                      ttGCTGAGCAATGGCAGGAGATAGACGGTTTTCCACGCATCATCTCAGGTATTAGCCATGGCGAAATCAGCCTGGTTTTAAATACGCTACGAGAGAAGGAACATTTTAAATTCACGACTTTtga                                                                                                                   >  4:821311/1‑124 (MQ=255)
                                                      ttGCTGAGCAATGGCAGGAGATAGACGGTTTTCCACGCATCAGCTCAGGTATTAGCCATGGCGAAATCAGCCTGGTTTTAAATACGCTACGAGGGAAGGAACATTTTAAATTCACGACTTTtga                                                                                                                   <  3:821311/124‑1 (MQ=255)
                                                          tGAGCAATGGCAGGAGATAGACGGTTTTCCACGCATCATCTCAGGTATTAGCCATGGCGAAATCAGCCTGGTTTTAAATACGCTACGAGAGAAAGAACATTTTAAATTCACGACTTTTGATGATGTCGACGATGCAACCATCGCCGAAt                                                                                      <  1:391403/149‑1 (MQ=255)
                                                                aTGGCAGGAGATAGACGGTTTTCCACGCATCATCTCAGGTATTAGCCATGGCGAAATCAGCCTGGTTTTAAATACGCTACGAGAGAAAGAACATTTTAAATTCACGACTTTTGATGATGTCGACGATGCAACCATCGCCGAATGGACgg                                                                                <  2:376935/149‑1 (MQ=255)
                                                                aTGGCAGGAGATAGACGGTTTTCCACGCATCATCTCAGGTATTAGCCATGGCGAAATCAGCCTGGTTTTAAATACGCTACGAGAGAAAGAACATTTTAAATTCACGACTTTTGATGATGTCGACGATGCAACCATCGCCGAATGGACgg                                                                                <  2:730161/149‑1 (MQ=255)
                                                                  ggCAGGAGATAGACGGTTTTCCACGCATCATCTCAGGTATTAGCCATGGCGAAATCAGCCTGGTTTTAAATACGCTACGAGAGAAAGAACATTTTAAATTCACGACTTTTGATGATGTCGACGATGCAACCATCGCCGAATGGACGGGa                                                                              <  2:177719/149‑1 (MQ=255)
                                                                   gCAGGAGATAGACGGTTTTCCACGCATCATCTCAGGTATTAGCCATGGCGAAATCAGCCTGGTTTTAAATACGCTACGAGAGAAAGAACATTTTAAATTCACGACTTTTGATGATGTCGACGATGCAACCATCGCCGAATGGACGGGAt                                                                             >  4:106659/1‑149 (MQ=255)
                                                                      ggAGATAGACGGTTTTCCACGCATCATCTCAGGTATTAGCCATGGCGAAATCAGCCTGGTTTTAAATACGCTACGCGAGAAAGAACATTTTAAATTCACGACTTTTGATGATGTCGACGATGCAACCATCGCCGAATGGACGGGa                                                                              >  4:332581/1‑145 (MQ=255)
                                                                      ggAGATAGACGGTTTTCCACGCATCATCTCAGGTATTAGCCATGGCGAAATCAGCCTGGTTTTAAATACGCTACGCGAGAAAGAACATTTTAAATTCACGACTTTTGATGATGTCGACGATGCAACCATCGCCGAATGGACGGGa                                                                              <  3:332581/145‑1 (MQ=255)
                                                                       gagaTAGACGGTTTTCCACGCATCATCTCAGGTATTAGCCATGGCGAAATCAGCCTGGTTTTAAATACGCTACGAGAGAAAGAACATTTTAAATTCACGACTTTTGATGATGTCGACGATGCAACCATCGCCGAATGGACGGGATTAAg                                                                         <  1:207070/149‑1 (MQ=255)
                                                                       gagaTAGACGGTTTTCCACGCATCATCTCAGGTATTAGCCATGGCGAAATCAGCCTGGTTTTAAATACGCTACGAGAGAAAGAACATTTTAAATTCACGACTTTTGATGATGTCGACGATGCAACCATCGCCGAATGGACGGGATTAAg                                                                         <  1:465911/149‑1 (MQ=255)
                                                                           tAGACGGTTTTCCACGCATCATCTCAGGTATTAGCCATGGCGAAATCAGCCTGGTTTTAAATACGCTACGAGAGAAAGAACATTTTAAATTCACGACTTTTGATGATGTCGACGATGCAACCATCGCCGAATGGACGGGATTAAGCCGt                                                                     >  4:388499/1‑149 (MQ=255)
                                                                           tAGACGGGTTTCCACGCATCATCTCAGGTATTAGCCATGGCGAAATCAGCCTGGTTTTAAATACGCTACGCGAGAAAGAACATTTTAAATTCACGACTTTTGATGATGTCGACGATGCAACCATCGCCGAATGGACGGGATTAAGCCGt                                                                     >  1:843414/1‑149 (MQ=255)
                                                                            aGACGGTTTTCCACGCATCATCTCAGGTATTAGCCATGGCGAAATCAGCCTGGTTTTAAATACGCTACGCGAGAAAGAACATTTTAAATTCACGACTTTTGATGATGTCGACGATGCAACCATCGCCGAATGGACGGGATTAAGCCGTa                                                                    <  3:401727/149‑1 (MQ=255)
                                                                             gACGGTTTTCCACGCATCATCTCAGGTATTAGCCATGGCGAAATCAGCCTGGTTTTAAATACGCTACGAGAGAAAGAACATTTTAAATTCACGACTTTTGATGATGTCGACGATGCAACCATCGCCGAATGGACGGGATTAAGCCGTAg                                                                   >  1:484147/1‑149 (MQ=255)
                                                                             gACGGTTTTCCACGCATCATCTCAGGTATTAGCCATGGCGAAATCAGCCTGGTTTTAAATACGCTACGAGAGAAAGAACATTTTAAATTCACGACTTTTGATGATGTCGACGATGCAACCATCGCCGAATGGACGGGATTAAGCCGTAg                                                                   >  3:453488/1‑149 (MQ=255)
                                                                              aCGGTTTTCCACGCATCATCTCAGGTATTAGCCATGGCGAAATCAGCCTGGTTTTAAATACGCTACGCGAGAAAGAACATTTTAAATTCACGACTTTtga                                                                                                                   >  2:657693/1‑100 (MQ=255)
                                                                              aCGGTTTTCCACGCATCATCTCAGGTATTAGCCATGGCGAAATCAGCCTGGTTTTAAATACGCTACGCGAGAAAGAACATTTTAAATTCACGACTTTtga                                                                                                                   <  1:657693/100‑1 (MQ=255)
                                                                              aCGGTTTTCCACGCATCATCTCAGGTATTAGCCATGGCGAAATCAGCCTGGTTTTAAATACGCTACGCGAGAAAGAACATTTTAAATTCACGACTTTTGATGATGTCGACGATGCAACCATCGCCGAATGGACGGGATTAAGCCGTAGc                                                                  >  1:632594/1‑149 (MQ=255)
                                                                              aCGGTTTTCCACGCATCATCTCAGGTATTAGCCATGGCGAAATCAGCCTGGTTTTAAATACGCTACGAGAGAAAGAACATTTTAAATTCACGACTTTTGATGATGTCGACGATGCAACCATCGCCGAATGGACGGGATTAAGcc                                                                       >  2:257331/1‑144 (MQ=255)
                                                                              aCGGTTTTCCACGCATCATCTCAGGTATTAGCCATGGCGAAATCAGCCTGGTTTTAAATACGCTACGAGAGAAAGAACATTTTAAATTCACGACTTTTGATGATGTCGACGATGCAACCATCGCCGAATGGACGGGATTAAGcc                                                                       <  1:257331/144‑1 (MQ=255)
                                                                              aCGGTTTTCCACGCATCATCTCAGGTATTAGCCATGGCGAAATCAGCCTGGTTTTAAATACGCTACGAGAGAAAGAACATTTTAAATTCACGACTTTTGATGATGTCGACGATGCAACCATCGCCGAATGGACGGGATTAAGCCGTAGc                                                                  <  4:746585/149‑1 (MQ=255)
                                                                              aCGGTTTTCCACGCATCATCTCAGGTATTAGCCATGGCGAAATCAGCCTGGTTTTAAATACGCTACGAGAGAAAGAACATTTTAAATTCACGACTTTTGATGATGTCGACGATGCAACCATCGCCGAATGGACGGGATTAAGCCGTAGc                                                                  >  4:82488/1‑149 (MQ=255)
                                                                                ggTTTTCCACGCATCATCTCAGGTATTAGCCATGGCGAAATCAGCCTGGTTTTAAATACGCTACGAGAGAAAGAACATTTTAAATTCACGACTTTTGATGATGTCGACGATGCAACCATCGCCGAATGGACGGGATTAAGCCGTAGCCa                                                                <  3:98301/149‑1 (MQ=255)
                                                                                 gTTTTCCACGCATCATCTCAGGTATTAGCCATGGCGAAATCAGCCTGGTTTTAAATACGCTACGAGAGAAAGAACATTTTAAATTCACGACTTTTGATGATGTCGACGATGCAACCATCGCCGAATGGACGGGATTAAGCCGTAGCCAg                                                               >  4:817220/1‑149 (MQ=255)
                                                                                   tttCCACGCATCATCTCAGGTATTAGCCATGGCGAAATCAGCCTGGTTTTAAATACGCTACGAGAGAAAGAACATTTTAAATTCACGACTTTTGATGATGTCGACGATGCAACCATCGCCGAATGGACGGGATTAAGCCGTAGCCAggc                                                             <  4:452913/149‑1 (MQ=255)
                                                                                      ccACGCATCATCTCAGGTATTAGCCATGGCGAAATCAGCCTGGTTTTAAATACGCTACGCGAGAAAGAACATTTTAAATTCACGACTTTTGATGATGTCGACGATGCAACCATCGCCGAATGGACGGGATTAAGCCGTAGCCAggcggc                                                          <  2:91130/149‑1 (MQ=255)
                                                                                       cACGCATCATCTCAGGTATTAGCCATGGCGAAATCAGCCTGGTTTTAAATACGCTACGAGAGAAAGAACATTTTAAATTCACGACTTTTGATGATGTCGACGATGCAACCATCGCCGAATGGACGGGATTAAGCCGTAGCCAGgcggcg                                                         >  4:274506/1‑149 (MQ=255)
                                                                                        aCGCATCATCTCAGGTATTAGCCATGGCGAAATCAGCCTGGTTTTAAATACGCTTCGAGAGAAAGAACATTTTAAATTCACGACTTTTGATGATGTCGACGATGCAACCATCGCCGAATGGACGGGATTAAGCCGTAGCCAGGCGgcgc                                                        >  2:218352/1‑149 (MQ=255)
                                                                                        aCGCATCATCTCAGGTATTAGCCATGGCGAAATCAGCCTGGTTTTAAATACGCTACGCGAGAAAGAACATTTTAAATTCACGACTTTTGATGATGTCGACGATGCAACCATCGCCGAATGGACGGGATTAAGCCGTAGCCAGGCGgcgc                                                        >  2:349060/1‑149 (MQ=255)
                                                                                                tctcAGGTATTAGCCATGGCGAAATCAGCCTGGTTTTAAATACGCTACGCGAGAAAGAACATTTTAAATTCACGACTTTTGATGATGTCGACGATGCAACCATCGCCGAATGGACGGGATTAAGCCGTAGCCAGGCGGCGCTGACGCAg                                                <  1:349060/149‑1 (MQ=255)
                                                                                                tctcAGGTATTAGCCATGGCGAAATCAGCCTGGTTTTAAATACGCTACGAGAGAAAGAACATTTTAAATTCACGACTTTTGATGATGTCGACGATGCAACCATCGCCGAATGGACGGGATTAAGCCGTAGCCAGGCGGCGCTGACGCAg                                                >  2:168418/1‑149 (MQ=255)
                                                                                                  tcAGGTATTAGCCATGGCGAAATCAGCCTGGTTTTAAATACGCTACGAGAGAAAGAACATTTTAAATTCACGACTTTTGATGATGTCGACGATGCAACCATCGCCGAATGGACGGGATTAAGCCGTAGCCAGGCGGCGCTGACGCAGCt                                              >  3:17/1‑149 (MQ=255)
                                                                                                    aGGTATTAGCCATGGCGAAATCAGCCTGGTTTTAAATACGCTACGCGAGAAAGAACATTTTAAATTCACGACTTTTGATGATGTCGACGATGCAACCATCGCCGAATGGACGGGATTAAGCCGTAGCCAGGCGGCGCTGACGCAGCTTc                                            >  1:179403/1‑149 (MQ=255)
                                                                                                      gTATTAGCCATGGCGAAATCAGCCTGGTTTTAAATACGCTACGCGAGAAAGAACATTTTAAATTCACGACTTTTGATGATGTCGACGATGCAACCATCGCCGAATGGACGGGATTAAGCCGTAGCCAGGCGGCGCTGACGCAGCTTCAt                                          >  3:352608/1‑149 (MQ=255)
                                                                                                      gTATTAGCCATGGCGAAATCAGCCTGGTTTTAAATACGCTACGAGAGAAAGAACATTTTAAATTCACGACTTTTGATGATGTCGACGATGCAACCATCGCCGAATGGACGGGATTAAGCCGTAGCCAGGCGGCGCTGACGCAGCTTCAt                                          <  4:161913/149‑1 (MQ=255)
                                                                                                       tATTAGCCATGGCGAAATCAGCCTGGTTTTAAATACGCTACGAGAGAAAGAACATTTTAAATTCACGACTTTTGATGATGTCGACGATGCAACCATCGCCGAATGGACgg                                                                                >  1:678072/1‑110 (MQ=255)
                                                                                                        aTTAGCCATGGCGAAATCAGCCTGGTTTTAAATACGCTACGCGAGAAAGAACATTTTAAATTCACGACTTTTGATGATGTCGACGATGCAACCATCGCCGAATGGACGGGATTAAGCCGTAGCCAGGCGGCGCTGACGCAGCTTCATGa                                        <  2:371646/149‑1 (MQ=255)
                                                                                                        aTTAGCCATGGCGAAATCAGCCTGGTTTTAAATACGCTACGAGAGAAAGAACATTTTAAATTCACGACTTTTGATGATGTCGACGATGCAACCATCGCCGAATGGACGGGATTAAGCCGTAGCCAGGCGGCGCTGACGCAGCTTCATGa                                        <  3:779520/149‑1 (MQ=255)
                                                                                                        aTTAGCCATGGCGAAATCAGCCTGGTTTTAAATACGCTACGAGAGAAAGAACATTTTAAATTCACGACTTTTGATGATGTCGACGATGCAACCATCGCCGAATGGACGGGATTAAGCCGTAGCCAGGCGGCGCTGACGCAGCTTCATGa                                        <  2:599332/149‑1 (MQ=255)
                                                                                                         ttAGCCATGGCGAAATCAGCCTGGTTTTAAATACGCTACGAGAGAAAGAACATTTTAAATTCACGACTTTTGATGATGTCGACGATGCAACCATCGCCGAATGGACGGGATTAAGCCGTAGCCAGGCGGCGCTGACGCAGCTTCATGAg                                       <  2:292570/149‑1 (MQ=255)
                                                                                                             ccATGGCGAAATCAGCCTGGTTTTAAATACGCTACGCGAGAAAGAACATTTTAAATTCACGACTTTTGATGATGTCGACGATGCAACCATCGCCGAATGGACGGGATTAAGCCGTAGCCAGGCGGCGCTGACGCAGCTTCATGAGGCGt                                   >  3:582240/1‑149 (MQ=255)
                                                                                                               aTGGCGAAATCAGCCTGGTTTTAAATACGCTACGAGAGAAAGAACATTTTAAATTCACGACTTTTGATGATGTCGACGATGCAACCATCGCCGAATGGACGGGATTAAGCCGTAGCCAGGCGGCGCTGACGCAGCTTCATGAGGCGTCg                                 <  1:241534/149‑1 (MQ=255)
                                                                                                                 ggCGAAATCAGCCTGGTTTTAAATACGCTACGCGAGAAAGAACATTTTAAATCCACGACTTTTGATGATGTCGACGATGCAACCATCGCCGAATGGACGGGATTAAGCCGTAGCCAGGCGGCGCTGACGCAGCTTCATGAGGCGTCGGt                               <  2:519872/149‑1 (MQ=255)
                                                                                                                  gCGAAATCAGCCTGGTTTTAAATACGCTACGCGAGAAAGAACATTTTAAATTCACGACTTTTGATGATGTCGACGATGCAACCATCGCCGAATGGACGGGATTAAGCCGTAGCCAGGCGGCGCTGACGCAGCTTCATGAGGCGTCGGTa                              >  1:51786/1‑149 (MQ=255)
                                                                                                                  gCGAAATCAGCCTGGTTTTAAATACGCTACGAGAGAAAGAACATTTTAAATTCACGACTTTTGATGATGTCGACGATGCAACCATCGCCGAATGGACGGGATTAAGCCGTAGCCAGGCGGCGCTGACGCAGCTTCATGAGGCGTCGGTa                              <  3:647785/149‑1 (MQ=255)
                                                                                                                       aTCAGCCTGGTTTTAAATACGCTACGCGAGAAAGAACATTTTAAATTCACGACTTTTGATGATGTCGACGATGCAACCATCGCCGAATGGACGGGATTAAGCCGTAGCCAGGCGGCGCTGACGCAGCTTCATGAGGCGTCGGTAACGCt                         >  3:813924/1‑149 (MQ=255)
                                                                                                                        tCAGCCTGGTTTTAAATACGCTACGAGAGAAAGAACATTTTAAATTCACGACTTTTGATGATGTCGACGATGCAACCATCGCCGAATGGACGGGATTAAGCCGTAGCCAGGCGGCGCTGACGCAGCTTCATGAGGCGTCGGTaa                             >  1:756015/1‑144 (MQ=255)
                                                                                                                        tCAGCCTGGTTTTAAATACGCTACGAGAGAAAGAACATTTTAAATTCACGACTTTTGATGATGTCGACGATGCAACCATCGCCGAATGGACGGGATTAAGCCGTAGCCAGGCGGCGCTGACGCAGCTTCATGAGGCGTCGGTaa                             <  2:756015/144‑1 (MQ=255)
                                                                                                                         cAGCCTGGTTTTAAATACGCTACGAGAGAAAGAACATTTTAAATTCACGACTTTTGATGATGTCGTCGATGCAACCATCGCCGAATAGACGGGATTAAGCCGTAGCCAGGCGGCGCTGACGCAGCTTCATGAGGCGTCGGTAACGCTaa                       <  4:523864/149‑1 (MQ=255)
                                                                                                                         cAGCCTGGTTTTAAATACGCTACGAGAGAAAGAACATTTTAAATTCACGACTTTTGATGATGTCGACGATGCAACCATCGCCGAATGGACGGGATTAAGCCGTAGCCAGGCGGCGCTGACGCAGCTTCATGAGGCGTCGGTAACGCTaa                       <  2:484147/149‑1 (MQ=255)
                                                                                                                         cAGCCTGGTTTTAAATACGCTACGAGAGAAAGAACATTTTAAATTCACGACTTTTGATGATGTCGACGATGCAACCATCGCCGAATGGACGGGATTAAGCCGTAGCCAGGCGGCGCTGACGCAGCTTCATGAGGCGTCGGTAACGCTaa                       <  4:453488/149‑1 (MQ=255)
                                                                                                                            ccTGGTTTTGAATACGCTACGCGAGAAAGAACATTTTAAATTCACGACTTTTGATGATGTCGACGATGCAACCATCGCCGAATGGACGGGATTAAGCCGTAGCCAGGCGGCGCTGACGCAGCTTCATGAGGCGTCGGTAACGCTAATCt                    >  1:590569/1‑149 (MQ=255)
                                                                                                                             cTGGTTTTAAATACGCTACGCGAGAAAGAACATTTTAAATTCACGACTTTTGATGATGTCGACGATGCAACCATTGCCGAATGGACGGGATTAAGCCGTAGCCAGGCGGCGCTGACGCAGCTTCATGAGGCGTCGGTAACGCTAATCTg                   >  2:537679/1‑149 (MQ=255)
                                                                                                                               ggTTTTAAATACGCTACGAGAGAAAGAACATTTTAAATTCACGACTTTTGATGATGTCGACGATGCAACCATCGCCGAATGGACGGGATTAAGCCGTAGCCAGGCGGCGCTGACGCAGCTTCGTGAGGCGTCGGTAACGCTAATCTGgc                 <  4:676347/149‑1 (MQ=255)
                                                                                                                                gTTTTAAATACGCTACGAGAGAAAGAACATTTTAAATTCACGACTTTTGATGATGTCGACGATGCAACCATCGCCGAATGGACGGGATTAAGCCGTAGCCAGGCGGCGCTGACGCAGCTTCATGAGGCGTCGGTAACGCTAATCTGgcg                >  3:133614/1‑149 (MQ=255)
                                                                                                                                 ttttAAATACGCTACGAGAGAAAGAACATTTTAAATTCACGACTTTTGATGATGTCGACGATGCAACCATCGCCGAATGGACGGGATTAAGCCGTAGCCAGGCGGCGCTGACGCAGCTTCATGAGGCGTCGGTAACGCTAATCTGgcgc               >  2:353488/1‑149 (MQ=255)
                                                                                                                                 ttttAAATACGCTACGAGAGAAAGAACATTTTAAATTCACGACTTTTGATGATGTCGACGATGCAACCATCGCCGAATGGACGGGATTAAGCCGTAGCCAGGCGGCGCTGACGCAGCTTCATGAGGCGTCGGTAACGCTAATCTGgcgc               >  2:215091/1‑149 (MQ=255)
                                                                                                                                   ttAAATACGCTACGAGAGAAAGAACATTTTAAATTCACGACTTTTGATGATGTCGACGATGCAACCATCGCCGAATGGACGGGATTAAGCCGTAGCCAGGCGGCGCTGACGCAGCTTCATGAGGCGTCGGTAACGCTAATCTGGCGCGa             <  3:106659/149‑1 (MQ=255)
                                                                                                                                     aaaTACGCTACGCGAGAAAGAACATTTTAAATTCACGACTTTTGATGATGTCGACGATGCAACCATCGCCGAATGGACGGGATTAAGCCGTAGCCAGGCGGCGCTGACGCAGCTTCATGAGGCGTCGGTAACGCTAATCTGGCGCGACa           >  4:502968/1‑149 (MQ=255)
                                                                                                                                       aTACGCTACGAGAGAAAGAACATTTTAAATTCACGACTTTTGATGATGTCGACGATGCAACCATCGCCGAATGGACGGGATTAAGCCGTAGCCAGGCGGCGCTGACGCAGCTTCATGAGGCGTCGGTAACGCTAATCTGGCGCGACAGt         <  2:339729/149‑1 (MQ=255)
                                                                                                                                         acgctacgaGAGAAAGAACATTTTAAATTCACGACTTTTGATGATGTCGACGATGCAACCATCGCCGAATGGACGGGATTAAGCCGTAGCCAGGCGGCGCTGACGCAGCTTCATGAGGCGTCGGTAACGCTAATCTGGCGCGACAGTGa       >  3:88198/1‑149 (MQ=255)
                                                                                                                                             tacgcGAGAAAGAACATTTTAAATTCACGACTTTTGATGATGTCGACGATGCAACCATCGCCGAATGGACGGGATTAAGCCGTAGCCAGGCGGCGCTGACGCAGCTTCATGAGGCGTCGGTAACGCTAATCTGGCGCGACAGTGACGAg   >  2:363973/1‑149 (MQ=255)
                                                                                                                                             tacgaGAGAAAGAACATTTTAAATTCACGACTTTTGATGATGTCGACGATGCAACCATCGCCGAATGGACGGGATTAAGCCGTAGCCAGGCGGCGCTGACGCAGCTTCATGAGGCGTCGGTAACGCTAATCTGGCGCGACAGTGACGAg   <  4:591890/149‑1 (MQ=255)
                                                                                                                                              acgcGAGAAAGAACATTTTAAATTCACGACTTTTGATGATGTCGACGATGCAACCATCGCCGAATGGACGGGATTAAGCCGTAGCCAGGCGGCGCTGACGCAGCTTCATGAGGCGTCGGTAACGCTAATCTGGCGCGACAGTGACGAGc  <  2:503440/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
CGTTGGGGCTACAAGGTTTACCGCTGATTGCCGAAAATGGCGCAGTGATCCAGCTTGCTGAGCAATGGCAGGAGATAGACGGTTTTCCACGCATCATCTCAGGTATTAGCCATGGCGAAATCAGCCTGGTTTTAAATACGCTACGCGAGAAAGAACATTTTAAATTCACGACTTTTGATGATGTCGACGATGCAACCATCGCCGAATGGACGGGATTAAGCCGTAGCCAGGCGGCGCTGACGCAGCTTCATGAGGCGTCGGTAACGCTAATCTGGCGCGACAGTGACGAGC  >  NC_000913/2025689‑2025979

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: