Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,282,375 C→T 100% H582Y (CAC→TAC)  yejH → putative ATP‑dependent DNA or RNA helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,282,3750CT100.0% 419.3 / NA 112H582Y (CAC→TAC) yejHputative ATP‑dependent DNA or RNA helicase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (54/58);  total (54/58)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 112 total aligned reads displayed.

TCCCGCTGCGCTGGATCACCGCCGCCGATATCCTCGCCCAGCAAGCCTTATTGCGACACCCGGATTTTGTCGTCGCCCGCATGAAAGGCCAGTACTGGCAGGTGCGTGAAAAAGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGCACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTAATTTAAGAGAGAAAGAAA  >  NC_000913/2282229‑2282517
                                                                                                                                                  |                                                                                                                                              
tCCCGCTGCGCTGGATCACCGCCGCCGATATCCTCGCCCAGCAAGCCTTATTGCGACACCCGGATTTTGTCGTCGCCCGCATGAAAGGCCAGTACTGGCAGGTGCGTGAAAAAGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGTAc                                                                                                                                              <  4:371058/149‑1 (MQ=255)
           tgGATCACCGCCGCCGATATCCTCGCCCAGCAAGCCTTATTGCGACACCCGGATTTTGTCGTCGCCCGCATGAAAGGCCAGTACTGGCAGGTGCGTGAAAAAGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGTACGAATTACGCgg                                                                                                                                   >  1:209817/1‑149 (MQ=255)
            ggATCACCGCCGCCGATATCCTCGCCCAGCAAGCCTTATTGCGACACCCGGATTTTGTCGTCGCCCGCATGAAAGGCCAGTACTGGCAGGTGCGTGAAAAAGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGTACGAATTACGCGGt                                                                                                                                  >  1:192935/1‑149 (MQ=255)
             gATCACCGCCGCCGATATCCTCGCCCAGCAAGCCTTATTGCGACACCCGGATTTTGTCGTCGCCCGCATGAAAGGCCAGTACTGGCAGGTGCGTGAAAAAGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGTACGAATTACGCGGtt                                                                                                                                 >  3:701787/1‑149 (MQ=255)
               tCACCGCCGCCGATATCCTCGCCCAGCAAGCCGTATTGCGACACCCGGATTTTGTCGTCGCCCGCATGAAAGGCCAGTACTGGCAGGTGCGTGAAAAAGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGTACGAATCACGCGGTTaa                                                                                                                               >  4:590561/1‑149 (MQ=255)
                 aCCGCCGCCGATATCCTCGCCCAGCAAGCCTTATTGCGACACCCGGATTTTGTCGTCGCCCGCATGAAAGGCCAGTACTGGCAGGTGCGTGAAAAAGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGTACGAATTACGCGGTTAATg                                                                                                                             >  3:667917/1‑149 (MQ=255)
                  ccgccgccgATATCCTCGCCCAGCAAGCCTTATTGCGACACCCGGATTTTGTCGTCGCCCGCATGAAAGGCCAGTACTGGCAGGTGCGTGAAAAAGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGTACGAATTACGCGGTTAATgg                                                                                                                            >  2:444212/1‑149 (MQ=255)
                   cgccgccgATATCCTCGCCGAGCAAGCCTTATTGCGACACCCGGATTTTGTCGTCGCCCGCATGAAAGGCCAGTACTGGCAGGTGCGTGAAAAAGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGTACGAATTACGCGGTTAATGGt                                                                                                                           >  4:237899/1‑149 (MQ=255)
                    gccgccgATATCCTCGCCCAGCAAGCCTTATTGCGACACCCGGATTTTGTCGTCGCCCGCATGAAAGGCCAGTACTGGCAGGTGCGTGAAAAAGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGTACGAATTACGCGGTTAATGGTa                                                                                                                          <  1:564844/149‑1 (MQ=255)
                    gccgccgATATCCTCGCCCAGCAAGCCTTATTGCGACACCCGGATTTTGTCGTCGCCCGCATGAAAGGCCAGTACTGGCAGGTGCGTGAAAAAGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGTACGAATTACGCGGTTAATGGTa                                                                                                                          <  2:540592/149‑1 (MQ=255)
                    gccgccgATATCCTCGCCCAGCAAGCCTTATTGCGACACCCGGATTTTGTCGTCGCCCGCATGAAAGGCCAGTACTGGCAGGTGCGTGAAAAAGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGTACGAATTACGCGGTTAATGGTa                                                                                                                          <  2:209817/149‑1 (MQ=255)
                     ccgccgATATCCTCGCCCAGCAAGCCTTATTGCGACACCCGGATTTTGTCGTCGCCCGCATGAAAGGCCAGTACTGGCAGGTGCGTGAAAAAGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGTACGAATTACGCGGTTAATGGTAg                                                                                                                         >  1:158264/1‑149 (MQ=255)
                       gccgATATCCTCGCCCAGCAAGCCTTATTGCGACACCCGGATTTTGTCGTCGCCCGCATGAAAGGCCAGTACTGGCAGGTGCGTGAAAAAGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGTACGAATTACGCGGTTAATGGTAGtt                                                                                                                       >  4:307535/1‑149 (MQ=255)
                        ccgATATCCTCGCCCAGCAAGCCTTATTGCGACACCCGGATTTTGTCGTCGCCCGCATGAAAGGCCAGTACTGGCAGGTGCGTGAAAAAGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGTACGAATTACGCGGTTAATGGTAGttt                                                                                                                      <  2:158264/149‑1 (MQ=255)
                           atatCCTCGCCCAGCAAGCCTTATTGCTACACCCGGATTTTGTCGTCGCCCGCATGAAAGGCCAGTACTGGCAGGTGCGTGAAAAAGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGTACGAATTACGCGGTTAATGGTAGTTttga                                                                                                                   <  1:276481/149‑1 (MQ=255)
                           atatCCTCGCCCAGCAAGCCTTATTGCGACACCCGGATTTTGTCGTCGCCCGCATGAAAGGCCAGTACTGGCAGGTGCGTGAAAAAGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGTACGAATTACGCGGTTAATGGTAGTTttga                                                                                                                   >  3:201714/1‑149 (MQ=255)
                               ccTCGCCCAGCAAGCCTTATTGCGACACCCGGATTTTGTCGTCGCCCGCATGAAAGGCCAGTACTGGCAGGTGCGTGAAAAAGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGTACGAATTACGCGGTTAATGGTAGTTttgattga                                                                                                               <  4:543310/149‑1 (MQ=255)
                               ccTCGCCCAGCAAGCCTTATTGCGACACCCGGATTTTGTCGTCGCCCGCATGAAAGGCCAGTACTGGCAGGTGCGTGAAAAAGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGTACGAATTACGCGGTTAATGGTAGTTttga                                                                                                                   >  3:350648/1‑145 (MQ=255)
                               ccTCGCCCAGCAAGCCTTATTGCGACACCCGGATTTTGTCGTCGCCCGCATGAAAGGCCAGTACTGGCAGGTGCGTGAAAAAGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGTACGAATTACGCGGTTAATGGTAGTTttga                                                                                                                   <  4:350648/145‑1 (MQ=255)
                                cTCGCCCAGCAAGCCTTATTGCGACACCCGGATTTTGTCGTCGCCCGCATGAAAGGCCAGTACTGGCAGGTGCGTGAAAAAGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGTACGAATTACGCGGTTAATGGTAGTTTtgattgat                                                                                                              <  3:210688/149‑1 (MQ=255)
                                cTCGCCCAGCAAGCCTTATTGCGACACCCGGATTTTGTCGTCGCCCGCATGAAAGGCCAGTACTGGCAGGTGCGTGAAAAAGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGTACGAATTACGCGGTTAATGGTAGTTTtgattgat                                                                                                              <  4:713088/149‑1 (MQ=255)
                                 tCGCCCAGCAAGCCTTATTGCGACACCCGGATTTTGTCGTCGCCCGCATGAAAGGCCAGTACTGGCAGGTGCGTGAAAAAGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGTACGAATTACGCGGTTAATGGTAGTTTTGATTGATg                                                                                                             >  2:203664/1‑149 (MQ=255)
                                 tCGCCCAGCAAGCCTTATTGCGACACCCGGATTTTGTCGTCGCCCGCATGAAAGGCCAGTACTGGCAGGTGCGTGAAAAAGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGTACGAATTACGCGGTTAATGGTAGTTTTGATTGATg                                                                                                             <  1:127228/149‑1 (MQ=255)
                                     ccAGCAAGCCTTATTGCGACACCCGGATTTTGTCGTCGCCCGCATGAAAGGCCAGTACTGGCAGGTGCGTGAAAAAGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGTACGAATTACGCGGTTAATGGTAGTTTTGATTGATGtata                                                                                                         >  1:937364/1‑149 (MQ=255)
                                      cAGCAAGCCTTATTGCGACACCCGGATTTTGTCGTCGCCCGCATGAAAGGCCAGTACTGGCAGGTGCGTGAAAAAGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGTACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATaa                                                                                                        >  4:699811/1‑149 (MQ=255)
                                            gCCTTATTGCGACACCCGGATTTTGTCGTCGCCCGCATGAAAGGCCAGTACTGGCAGGTGCGTGAAAAAGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGTACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGAt                                                                                                  >  1:387139/1‑149 (MQ=255)
                                            gCCTTATTGCGACACCCGGATTTTGTCGTCGCCCGCATGAAAGGCCAGTACTGGCAGGTGCGTGAAAAAGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGTACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGAt                                                                                                  >  3:128258/1‑149 (MQ=255)
                                              cTTATTGCGACACCCGGATTTTGTCGTCGCCCGCATGAAAGGCCAGTACTGGCAGGTGCGTGAAAAAGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGTACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTg                                                                                                >  2:199124/1‑149 (MQ=255)
                                               ttATTGCGACACCCGGATTTTGTCGTCGCCCGCATGAAAGGCCAGTACTGGCAGGTGCGTGAAAAAGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGTACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGa                                                                                               <  1:783411/149‑1 (MQ=255)
                                                tATTGCGACACCCGGATTTTGTCGTCGCCCGCATGAAAGGCCAGTACTGGCAGGTGCGTGAAAAAGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGTACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAg                                                                                              <  4:463676/149‑1 (MQ=255)
                                                 aTTGCGACACCCGGATTTTGTCGTCGCCCGCATGAAAGGCCAGTACTGGCAGGTGCGTGAAAAAGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGTACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGt                                                                                             >  4:329386/1‑149 (MQ=255)
                                                 aTTGCGACACCCGGATTTTGTCGTCGCCCGCATGAAAGGCCAGTACTGGCAGGTGCGTGAAAAAGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGTACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGt                                                                                             >  3:881737/1‑149 (MQ=255)
                                                  ttGCGACACCCGGATTTTGTCGTCGCCCGCATGAAAGGCCAGTACTGGCAGGTGCGTGAAAAAGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGTACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGta                                                                                            >  4:321187/1‑149 (MQ=255)
                                                      gACACCCGGATTTTGTCGTCGCCCGCATGAAAGGCCAGTACTGGCAGGTGCGTGAAAAAGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGTACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGa                                                                                        >  3:244711/1‑149 (MQ=255)
                                                       acacCCGGATTTTGTCGTCGCCCGCATGAAAGGCCAGTACTGGCAGGTGCGTGAAAAAGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGTACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGaa                                                                                       <  3:745185/149‑1 (MQ=255)
                                                       acacCCGGATTTTGTCGTCGCCCGCATGAAAGGCCAGTACTGGCAGGTGCGTGAAAAAGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGTACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGaa                                                                                       <  3:590561/149‑1 (MQ=255)
                                                           ccGGATTTTGTCGTCGCCCGCATGAAAGGCCAGTACTGGCAGGTGCGTGAAAAAGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGTACGAATTACGCGGTTAATGGTAGTTTtgattgat                                                                                                              <  2:849272/122‑1 (MQ=255)
                                                           ccGGATTTTGTCGTCGCCCGCATGAAAGGCCAGTACTGGCAGGTGCGTGAAAAAGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGTACGAATTACGCGGTTAATGGTAGTTTtgattgat                                                                                                              >  1:849272/1‑122 (MQ=255)
                                                           ccGGATTTTGTCGTCGCCCGCATGAAAGGCCAGTACTGGCAGGTGCGTGAAAAAGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGTACGAATTACGCGGTTAATGGTAGTTTTGATTGATg                                                                                                             >  4:508081/1‑123 (MQ=255)
                                                           ccGGATTTTGTCGTCGCCCGCATGAAAGGCCAGTACTGGCAGGTGCGTGAAAAAGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGTACGAATTACGCGGTTAATGGTAGTTTTGATTGATg                                                                                                             <  3:508081/123‑1 (MQ=255)
                                                           ccGGATTTTGTCGTCGCCCGCATGAAAGGCCAGTACTGGCAGGTGCGTGAAAAAGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGTACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAAtctc                                                                                   >  3:268044/1‑149 (MQ=255)
                                                           ccGGATTTTGTCGTCGCCCGCATGAAAGGCCAGTACTGGCAGGTGCGTGAAAAAGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGTACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAAtctc                                                                                   >  2:604810/1‑149 (MQ=255)
                                                                ttttGTCGTCGCCCGCATGAAAGGCCAGTACTGGCAGGTGCGTGAAAAAGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGTACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGa                                                                                               >  3:592537/1‑132 (MQ=255)
                                                                ttttGTCGTCGCCCGCATGAAAGGCCAGTACTGGCAGGTGCGTGAAAAAGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGTACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGa                                                                                               <  4:592537/132‑1 (MQ=255)
                                                                     tcgtcgCCCGCATGAAAGGCCAGTACTGGCAGGTGCGTGAAAAAGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGTACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCtttt                                                                         >  2:833509/1‑149 (MQ=255)
                                                                       gtcgCCCGCATGAAAGGCCAGTACTGGCAGGTGCGTGAAAAAGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGTACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGAt                                                                                                  <  1:541675/122‑1 (MQ=255)
                                                                       gtcgCCCGCATGAAAGGCCAGTACTGGCAGGTGCGTGAAAAAGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGTACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGAt                                                                                                  >  2:541675/1‑122 (MQ=255)
                                                                       gtcgCCCGCATGAAAGGCCAGTACTGGCAGGTGCGTGAAAAAGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGTACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGc                                                                       <  4:268044/149‑1 (MQ=255)
                                                                          gCCCGCATGAAAGGCCAGTACTGGCAGGTGCGTGAAAAAGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGTACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCAt                                                                     >  2:920479/1‑148 (MQ=255)
                                                                          gCCCGCATGAAAGGCCAGTACTGGCAGGTGCGTGAAAAAGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGTACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCAt                                                                     <  1:920479/148‑1 (MQ=255)
                                                                          gCCCGCATGAAAGGCCAGTACTGGCAGGTGCGTGAAAAAGGGTTCGATTACGAAGGTCGTTTTCGTCTGGCGTACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCAt                                                                     >  4:504756/1‑148 (MQ=255)
                                                                           cccGCATGAAAGGCCAGTACTGGCAGGTGCGTGAAAAAGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGTACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATAc                                                                   <  1:156139/149‑1 (MQ=255)
                                                                            ccGCATGAAAGGCCAGTACTGGCAGGTGCGTGAAAAAGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGTACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACg                                                                  >  3:595242/1‑149 (MQ=255)
                                                                            ccGCATGAAAGGCCAGTACTGGCAGGTGCGTGAAAAAGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGTACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACg                                                                  >  4:123/1‑149 (MQ=255)
                                                                            ccGCATGAAAGGCCAGTACTGGCAGGTGCGTGAAAAAGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGTACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACg                                                                  >  1:323621/1‑149 (MQ=255)
                                                                             cGCATGAAAGGCCAGTACTGGCAGGTGCGTGAAAAAGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGTACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAACCTCGCCCGCTTTTGCATACGc                                                                 >  4:872772/1‑149 (MQ=255)
                                                                                aTGAAAGGCCAGTACTGGCAGGTGCGTGAAAAAGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGTACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCaaa                                                              <  1:894622/149‑1 (MQ=255)
                                                                                 tGAAAGGCCAGTACTGGCAGGTGCGTGAAAAAGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGTACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAg                                                             <  1:371907/149‑1 (MQ=255)
                                                                                  gAAAGGCCAGTACTGGCAGGTGCGTGAAAAAGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGTACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGc                                                            >  1:328724/1‑149 (MQ=255)
                                                                                   aaaGGCCAGTACTGGCAGGTGCGTGAAAAAGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGTACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCa                                                           <  4:602956/149‑1 (MQ=255)
                                                                                     aGGCCAGTACTGGCAGGTGCGTGAAAAAGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGTACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGa                                                         <  1:438810/149‑1 (MQ=255)
                                                                                         cAGTACTGGCAGGTGCGTGAAAAAGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGTACGAATTACGCGGTTAATGGTAGTTttga                                                                                                                   <  4:365688/87‑1 (MQ=255)
                                                                                         cAGTACTGGCAGGTGCGTGAAAAAGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGTACGAATTACGCGGTTAATGGTAGTTttga                                                                                                                   >  3:365688/1‑87 (MQ=255)
                                                                                          aGTACTGGCAGGTGCGTGAAAAAGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGTACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACt                                                    <  1:203664/149‑1 (MQ=255)
                                                                                                 gCAGGTGCGTGAAAAAGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGTACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCAc                                                     <  1:148825/141‑1 (MQ=255)
                                                                                                 gCAGGTGCGTGAAAAAGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGTACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCAc                                                     >  2:148825/1‑141 (MQ=255)
                                                                                                 gCAGGTGCGTGAAAAAGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGTACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGt                                             >  3:103581/1‑149 (MQ=255)
                                                                                                 gCAGGTGCGTGAAAAAGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGTACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGt                                             >  2:282884/1‑149 (MQ=255)
                                                                                                 gCAGGTGCGTGAAAAAGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGTACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGt                                             >  2:224605/1‑149 (MQ=255)
                                                                                                       gCGTGAAAAAGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGTACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTgg                                       <  4:128258/149‑1 (MQ=255)
                                                                                                       gCGTGAAAAAGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGTACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTgg                                       <  2:728539/149‑1 (MQ=255)
                                                                                                         gTGAAAAAGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGTACGAATTACGCGGTTAATGGTAGTTTtgattgat                                                                                                              <  1:364990/76‑1 (MQ=255)
                                                                                                         gTGAAAAAGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGTACGAATTACGCGGTTAATGGTAGTTTtgattgat                                                                                                              >  2:364990/1‑76 (MQ=255)
                                                                                                         gTGAAAAAGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGTACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGt                                     <  2:645271/149‑1 (MQ=255)
                                                                                                         ctGAAAAAGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGTACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGt                                     <  2:820054/148‑1 (MQ=255)
                                                                                                            aaaaaGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGTACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGc                                  <  1:645876/149‑1 (MQ=255)
                                                                                                            aaaaaGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGTACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGc                                  <  3:321187/149‑1 (MQ=255)
                                                                                                            aaaaaGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGTACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGc                                  >  1:800131/1‑149 (MQ=255)
                                                                                                            aaaaaGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGTACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGc                                  >  2:3731/1‑149 (MQ=255)
                                                                                                             aaaaGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGTACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGcc                                 >  4:280883/1‑149 (MQ=255)
                                                                                                              aaaGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGTACGAATTACGCGGTTAATGGTAGTTttgatt                                                                                                                 >  2:620329/1‑68 (MQ=255)
                                                                                                              aaaGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGTACGAATTACGCGGTTAATGGTAGTTttgatt                                                                                                                 <  1:620329/68‑1 (MQ=255)
                                                                                                              aaaGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGTACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCt                                >  3:280719/1‑149 (MQ=255)
                                                                                                                 gtgtTCGATTACGAAGGTCGTTTTCGTCTGGCGTACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTa                             <  3:175847/149‑1 (MQ=255)
                                                                                                                  tgtTCGATTACGAAGGTCGTTTTCGTCTGGCGTACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAg                            >  1:338527/1‑149 (MQ=255)
                                                                                                                        aTTACGAAGGTCGTTTTCGTCTGGCGTACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGAt                      <  1:604810/149‑1 (MQ=255)
                                                                                                                         ttACGAAGGTCGTTTTCGTCTGGCGTACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGAtt                     >  3:346303/1‑149 (MQ=255)
                                                                                                                          tACGAAGGTCGTTTTCGTCTGGCGTACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGcc                                 <  1:297869/136‑1 (MQ=255)
                                                                                                                          tACGAAGGTCGTTTTCGTCTGGCGTACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGcc                                 >  2:297869/1‑136 (MQ=255)
                                                                                                                          tACGAAGGTCGTTTTCGTCTGGCGTACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTa                    <  4:701787/149‑1 (MQ=255)
                                                                                                                           aCGAAGGTCGTTTTCGTCTGGCGTACGAATTACGCGGTTAATGGTAGGTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTaa                   <  2:202851/149‑1 (MQ=255)
                                                                                                                            cGAAGGTCGTTTTCGTCTGGCGTACGAATTACGCGGTTAATGGTAGTTTTGATTTATGTATAAGGCGATTGAGTATAGAATCTCGCCAGCTTTTGCATACACAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTAAt                  <  4:881737/149‑1 (MQ=255)
                                                                                                                            cGAAGGTCGTTTTCGTCTGGCGTACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTAAt                  <  3:463239/149‑1 (MQ=255)
                                                                                                                               aGGTCGTTTTCGTCTGGCGTACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTAATTTa               >  3:80849/1‑149 (MQ=255)
                                                                                                                                ggTCGTTTTCGTCTGGCGTACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTAATTTaa              <  2:294910/149‑1 (MQ=255)
                                                                                                                                ggTCGTTTTCGTCTGGCGTACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTAATTTaa              <  2:411048/149‑1 (MQ=255)
                                                                                                                                ggTCGTTTTCGTCTGGCGTACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTAATTTaa              <  1:190086/149‑1 (MQ=255)
                                                                                                                                    gTTTTCGTCTGGCGTACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTAATTTAagaga          <  4:782084/149‑1 (MQ=255)
                                                                                                                                        tCGTCTGGCGTACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTAATTTAAGAGagaaa      <  2:338527/149‑1 (MQ=255)
                                                                                                                                            cTGGCGTACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTAATTTAAGAGAgaaagaaa  <  2:328724/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                              
TCCCGCTGCGCTGGATCACCGCCGCCGATATCCTCGCCCAGCAAGCCTTATTGCGACACCCGGATTTTGTCGTCGCCCGCATGAAAGGCCAGTACTGGCAGGTGCGTGAAAAAGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGCACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTAATTTAAGAGAGAAAGAAA  >  NC_000913/2282229‑2282517

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: