Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,302,295 G→T 100% I153I (ATC→ATA napA ← nitrate reductase, periplasmic, large subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,302,2950GT100.0% 367.9 / NA 99I153I (ATC→ATAnapAnitrate reductase, periplasmic, large subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (48/51);  total (48/51)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 101 total aligned reads displayed.

CGTCATAGCAGCCCATCGGCTCATCCATACCAAAGGTACGCATAAAGCCAACTACTGCCGACGCCATACAGTGACGCGCGTTCGGGTCGATGTTGTTCGAACGGAAGCCCGCTTTAAACAGCTTGGACGCGGCATAACCTTCCCAGATAGTCCACTGACCAGAACCGAACATACCGATCGATTCCGGCCCTTTTTCTTTCAGGGCGGTTTTGAATTTCTCTTCCATCACATCGAAGGCCTGATCCCAGGTGATTGGGGTAAATTCGCCTTCTTTGTCATATTTACCG  >  NC_000913/2302150‑2302436
                                                                                                                                                 |                                                                                                                                             
cgtcATAGCAGCCCATCGGCTCATCCATACCAAAGGTACGCATAAAGCCAACTACTGCCGACGCCATACAGTGACGCGCGTTCGGGTCGATGTTGTTCGAACGGAAGCCCGCTTTAAACAGCTTGGACGCGGCATAACCTTCCCAtata                                                                                                                                            <  1:654914/149‑1 (MQ=255)
cgtcATAGCAGCCCATCGGCTCATCCATACCAAAGGTACGCATAAAGCCAACTACTGCCGACGCCATACAGTGACGCGCGTTCGGGTCGATGTTGTTCGAACGGAAGCCCGCTTTAAACAGCTTGGACGCGGCATAACCTTCCCAtata                                                                                                                                            <  1:909083/149‑1 (MQ=255)
 gtcATAGCAGCCCATCGGCTCATCCATACCAAAGGTACGCATAAAGCCAACTACTGCCGACGCCATACAGTGACGCGCGTTCGGGTCGATGTTGTTCGAACGGAAGCCCGCTTTAAACAGCTTGGACGCGGCATAACCTTCCCATATAg                                                                                                                                           >  2:692435/1‑149 (MQ=255)
       gcagcCCATCGGCTCATCCATACCAAAGGTACGCATAAAGCCAACTACTGCCGACGCCATACAGTGACGCGCGTTCGGGTCGATGTTGTTCGAACGGAAGCCCGCTTTAAACAGCTTGGACGCGGCATAACCTTCCCATATAGTCCACt                                                                                                                                     <  3:657505/149‑1 (MQ=255)
         agcCCATCGGCTCATCCATACCAAAGGTACGCATAAAGCCAACTACTGCCGACGCCATACAGTGACGCGCGTTCGGGTCGATGTTGTTCGAACGGAAGCCCGCTTTAAACAGCTTGGACGCGGCATAACCTTCCCATATAGTCCACTGa                                                                                                                                   <  2:66886/149‑1 (MQ=255)
          gcCCATCGGCTCATCCATACCAAAGGTACGCATAAAGCCAACTACTGCCGACGCCATACAGTGACGCGCGTTCGGGTCGATGTTGTTCGAACGGAAGCCCGCTTTAAACAGCTTGGACGCGGCATAACCTTCCCATATAGTCCACTGAc                                                                                                                                  <  1:395893/149‑1 (MQ=255)
              aTCGGCTCATCCATACCAAAGGTACGCATAAAGCCAACTACTGCCGACGCCATACAGTGACGCGCGTTCGGGTCGATGTTGTTCGAACGGAAGCCCGCTTTAAACAGCTTGGACGCGGCATAACCTTCCCATATAGTCCACTGACCAGa                                                                                                                              >  1:811887/1‑149 (MQ=255)
                    tCATCCATACCAAAGGTACGCATAAAGCCAACTACTGCCGACGCCATACAGTGACGCGCGTTCGGGTCGATGTTGTTCGAACGGAAGCCCGCTTTAAACAGCTTGGACGCGGCATAACCTTCCCATATAGTCCACTGAc                                                                                                                                  <  1:741852/139‑1 (MQ=255)
                    tCATCCATACCAAAGGTACGCATAAAGCCAACTACTGCCGACGCCATACAGTGACGCGCGTTCGGGTCGATGTTGTTCGAACGGAAGCCCGCTTTAAACAGCTTGGACGCGGCATAACCTTCCCATATAGTCCACTGAc                                                                                                                                  >  2:741852/1‑139 (MQ=255)
                    tCATCCATACCAAAGGTACGCATAAAGCCAACTACTGCCGACGCCATACAGTGACGCGCGTTCGGGTCGATGTTGTTCGAACGGAAGCCCGCTTTAAACAGCTTGGACGCGGCATAACCTTCCCATATAGTCCACTGACCAGAACCGaa                                                                                                                        >  4:446924/1‑149 (MQ=255)
                    tCATCCATACCAAAGGTACGCATAAAGCCAACTACTGCCGACGCCATACAGTGACGCGCGTTCGGGTCGATGTTGTTCGAACGGAAGCCCGCTTTAAACAGCTTGGACGCGGCATAACCTTCCCATATAGTCCACTGACCAGAACCGaa                                                                                                                        >  1:347524/1‑149 (MQ=255)
                      aTCCATACCAAAGGTACGCATAAAGCCAACTACTGCCGACGCCATACAGTGACGCGCGTTCGGGTCGATGTTGTTCGAACGGACGCCCGCTTTAAACAGCTTGGACGCGGCATAACCTTCCCATATAGTCCACt                                                                                                                                     <  2:278078/134‑1 (MQ=255)
                      aTCCATACCAAAGGTACGCATAAAGCCAACTACTGCCGACGCCATACAGTGACGCGCGTTCGGGTCGATGTTGTTCGAACGGAAGCCCGCTTTAAACAGCTTGGACGCGGCATAACCTTCCCATATAGTCCACt                                                                                                                                     >  1:278078/1‑134 (MQ=255)
                      aTCCATACCAAAGGTACGCATAAAGCCAACTACTGCCGACGCCATACAGTGACGCGCGTTCGGGTCGATGTTGTTCGAACGGAAGCCCGCTTTAAACAGCTTGGACGCGGCATAACCTTCCCATATAGTCCACTGACCAGAACCGAACa                                                                                                                      >  1:353024/1‑149 (MQ=255)
                       tCCATACCAAAGGTACGCATAAAGCCAACTACTGCCGACGCCATACAGTGACGCGCGTTCGGGTCGATGTTGTTCGAACGGAAGCCCGCTTTAAACAGCTTGGACGCGGCATAACCTTCCCATATAGTCCACTGACCAGAACCGAACAt                                                                                                                     >  2:356978/1‑149 (MQ=255)
                         cATACCAAAGGTACGCATAAAGCCAACTACTGCCGACGCCATACAGTGACGCGCGTTCGGGTCGATGTTGTTCGAACGGAAGCCCGCTTTAAACAGCTTGGACGCGGCATAACCTTCCCATATAGTCCACTGACCAGAACCGAACATAc                                                                                                                   >  1:301730/1‑149 (MQ=255)
                              cAAAGGTACGCATAAAGCCAACTACTGCCGACGCCATACAGTGACGCGCGTTCGGGTCGATGTTGTTCGAACGGAAGCCCGCTTTAAACAGCTTGGACGCGGCATAACCTTCCCATATAGTCCACTGACCAGAACCGAACATACcgatc                                                                                                              <  1:58378/149‑1 (MQ=255)
                              cAAAGGTACGCATAAAGCCAACTACTGCCGACGCCATACAGTGACGCGCGTTCGGGTCGATGTTGTTCGAACGGAAGCCCGCTTTAAACAGCTTGGACGCGGCATAACCTTCCCATATAGTCCACTGACCAGAACCGAACATACcgatc                                                                                                              >  3:774005/1‑149 (MQ=255)
                                      cGCATAAAGCCAACTACTGCCGACGCCATACAGTGACGCGCGTTCGGGTCGATGTTGTTCGAACGGAAGCCCGCTTTAAACAGCTTGGACGCGGCATAACCTTCCCATATAGTCCACTGACCAGAACCGAACATACCGATCGATTCCgg                                                                                                      <  1:6851/149‑1 (MQ=255)
                                       gCATAAAGCCAACTACTGCCGACGCCATACAGTGACGCGCGTTCGGGTCTATGTTGTTCGAACGGAAGCCCGCTTTAAACAGCTTGGACGCGGCATAACCTTCCCATAGAGGCCACTTACCAGAACCGAACATACCGACCGATTCCGGc                                                                                                     >  4:511472/1‑149 (MQ=255)
                                       gCATAAAGCCAACTACTGCCGACGCCATACAGTGACGCGCGTTCGGGTCGATGTTGTTCGAACGGAAGCCCGCTTTAAACAGCTTGGACGCGGCATAACCTTCCCATATAGTCCACTGACCAGAACCGAACATACCGATCGATTCCGGc                                                                                                     <  4:17389/149‑1 (MQ=255)
                                         aTAAAGCCAACTACTGCCGACGCCATACAGTGACGCGCGTTCGGGTCGATGTTGTTCGAACGGAAGCCCGCTTTAAACAGCTTGGACGCGGCATAACCTTCCCATATAGTCCACTGACCAGAACCGAACATACCGATCGATTCCGGccc                                                                                                   >  3:695629/1‑149 (MQ=255)
                                          tAAAGCCAACTACTGCCGACGCCATACAGTGACGCGCGTTCGGGTCGATGTTGTTCGAACGGAAGCCCGCTTTAAACAGCTTGGACGCGGCATAACCTTCCCATATAGTCCACTGACCAGAACCGAACATACCGATCGATTCCGGCCCt                                                                                                  >  3:500750/1‑149 (MQ=255)
                                            aaGCCAACTACTGCCGACGCCATACAGTGACGCGCGTTCGGGTCGATGTTGTTCGAACGGAAGCCCGCTTTAAACAGCTTGGACGCGGCATAACCTTCCCATATAGTCCACTGACCAGAACCGAACATACCGATCGATTCCGGCCCttt                                                                                                >  3:688352/1‑149 (MQ=255)
                                             aGCCAACTACTGCCGACGCCATACAGTGACGCGCGTTCGGGTCGATGTTGTTCGAACGGAAGCCCGCTTTAAACAGCTTGGACGCGGCATAACCTTCCCATATAGTCCACTGACCAGAACCGAACATACCGATCGATTCCGGCCCtttt                                                                                               >  2:459064/1‑149 (MQ=255)
                                              gCCAACTACTGCCGACGCCATACAGTGACGCGCGTTCGGGTCGATGTTGTTCGAACGGAAGCCCGCTTTAAACAGCTTGGACGCGGCATAACCTTCCCATATAGTCCACTGACCAGAACCGAACATACCGATCGATTCCGGCCCttttt                                                                                              <  1:90921/149‑1 (MQ=255)
                                              gCCAACTACTGCCGACGCCATACAGTGACGCGCGTTCGGGTCGATGTTGTTCGAACGGAAGCCCGCTTTAAACAGCTTGGACGCGGCATAACCTTCCCATATAGTCCACTGACCAGAACCGAACATACCGATCGATTCCGGCCCttttt                                                                                              <  4:411092/149‑1 (MQ=255)
                                               ccAACTACTGCCGACGCCATACAGTGACGCGCGTTCGGGTCGATGTTGTTCGAACGGAAGCCCGCTTTAAACAGCTTGGACGCGGCATAACCTTCCCATATAGTCCACTGACCAGAACCGAACATACCGATCGATTCCGGCCCTTtttc                                                                                             >  2:627525/1‑149 (MQ=255)
                                               ccAACTACTGCCGACGCCATACAGTGACGCGCGTTCGGGTCGATGTTGTTCGAACGGAAGCCCGCTTTAAACAGCTTGGACGCGGCATAACCTTCCCATATAGTCCACTGACCAGAACCGAACATACCGATCGATTCCGGCCCTTtttc                                                                                             <  1:927498/149‑1 (MQ=255)
                                                 aaCTACTGCCGACGCCATACAGTGACGCGCGTTCGGGTCGATGTTGTTCGAACGGAAGCCCGCTTTAAACAGCTTGGACGCGGCATAACCTTCCCATATAGTCCACTGACCAGAACCGAACATACCGATCGATTCCGGCCCTTtttctt                                                                                           >  4:825253/1‑149 (MQ=255)
                                                 aaCTACTGCCGACGCCATACAGTGACGCGCGTTCGGGTCGATGTTGTTCGAACGGAAGCCCGCTTTAAACAGCTTGGACGCGGCATAACCTTCCCATATAGTCCACTGACCAGAACCGAACATACCGATCGATTCCGGCCCTTtttctt                                                                                           >  1:700493/1‑149 (MQ=255)
                                                 aaCTACTGCCGACGCCATACAGTGACGCGCGTTCGGGTCGATGTTGTTCGAACGGAAGCCCGCTTTAAACAGCTTGGACGCGGCATAACCTTCCCATATAGTCCACTGACCAGAACCGAACATACCGATCGATTCCGGCCCTTtttctt                                                                                           >  1:619256/1‑149 (MQ=255)
                                                    tactGCCGACGCCATACAGTGACGCGCGTTCGGGTCGATGTTGTTCGAACGGAAGCCCGCTTTAAACAGCTTGGACGCGGCATAACCTTCCCATATAGTCCACTGACCAGAACCGAACATACCGATCGATTCCGGCCCTTTTTCTTTCa                                                                                        <  1:597398/149‑1 (MQ=255)
                                                    tactGCCGACGCCATACAGTGACGCGCGTTCGGGTCGATGTTGTTCGAACGGAAGCCCGCTTTAAACAGCTTGGACGCGGCATAACCTTCCCATATAGTCCACTGACCAGAACCGAACATACCGATCGATTCCGGCCCTTTTTCTTTCa                                                                                        <  3:66955/149‑1 (MQ=255)
                                                        gCCGACGCCATACAGTGACGCGCGTTCGGGTCGATGTTGTTCGAACGGAAGCCCGCTTTAAACAGCTTGGACGCGGCATAACCTTCCCATATAGTCCACTGACCAGAACCGAACATACCGATCGATTCCGGCCCTTTTTCTTTCAGGGc                                                                                    <  3:840354/149‑1 (MQ=255)
                                                             cGCCATACAGTGACGCGCGTTCGGGTCGATGTTGTTCGAACGGAAGCCCGCTTTAAACAGCTTGGACGCGGCATAACCTTCCCATATAGTCCACTGACCAGAACCGAACATACCGATCGATTCCGGCCCTTTTTCTTTCAGGGCGGttt                                                                               <  3:825253/149‑1 (MQ=255)
                                                               ccATACAGTGACGCGCGTTCGGGTCGATGTTGTTCGAACGGAAGCCCGCTTTAAACAGCTTGGACGCGGCATAACCTTCCCATATAGTCCACTGACCAGAACCGAACATACCGATCGATTCCGGCCCTTTTTCTTTCAGGGCGGTTTTg                                                                             >  3:199517/1‑149 (MQ=255)
                                                                cATACAGTGACGCGCGTTCGGGTCGATGTTGTTCGAACGGAAGCCCGCTTTAAACAGCTTGGACGCGGCATAACCTTCCCATATAGTCCACTGACCAGAACCGAACATACCGATCGATTCCCGCCCTTTTTCTTTCAGGGCGGTTTTGa                                                                            <  4:832525/149‑1 (MQ=255)
                                                                 aTACAGTGACGCGCGTTCGGGTCGATGTTGTTCGAACGGAAGCCCGCTTTAAACAGCTTGGACGCGGCATAACCTTCCCATATAGTCCACTGACCAGAACCGAACATACCGATCGATTCCGGCCCTTTTTCTTTCAGGGCGGtttt                                                                              >  2:570948/1‑146 (MQ=255)
                                                                 aTACAGTGACGCGCGTTCGGGTCGATGTTGTTCGAACGGAAGCCCGCTTTAAACAGCTTGGACGCGGCATAACCTTCCCATATAGTCCACTGACCAGAACCGAACATACCGATCGATTCCGGCCCTTTTTCTTTCAGGGCGGtttt                                                                              <  1:570948/146‑1 (MQ=255)
                                                                 aTACAGTGACGCGCGTTCGGGTCGATGTTGTTCGAACGGAAGCCCGCTTTAAACAGCTTGGACGCGGCATAACCTTCCCATATAGTCCACTGACCAGAACCGAACATACCGATCGATTCCGGCCCTTTTTCTTTCAGGGCGGTTTTGaa                                                                           <  2:783709/149‑1 (MQ=255)
                                                                 aTACAGTGACGCGCGTTCGGGTCGATGTTGTTCGAACGGAAGCCCGCTTTAAACAGCTTGGACGCGGCATAACCTTCCCATATAGTCCACTGACCAGAACCGAACATACCGATCGATTCCGGCCCTTTTTCTTTCAGGGCGGTTTTGaa                                                                           >  1:831781/1‑149 (MQ=255)
                                                                 aTACAGTGACGCGCGTTCGGGTCGATGTTGTTCGAACGGAAGCCCGCTTTAAACAGCTTGGACGCGGCATAACCTTCCCATATAGTCCACTGACCAGAACCGAACATACCGATCGATTCCGGCCCTTTTTCTTTCAGGGCGGTTTTGaa                                                                           <  2:353024/149‑1 (MQ=255)
                                                                 aTACAGTGACGCGCGTTCGGGTCGATGTTGTTCGAACGGAAGCCCGCTTTAAACAGCTTGGACGCGGCATAACCTTCCCATATAGTCCACTGACCAGAACCGAACATACCGATCGATTCCGGCCCTTTTTCTTTCAGGGCGGTTTTGaa                                                                           <  1:125034/149‑1 (MQ=255)
                                                                 aTACAGTGACGCGCGTTCGGGTCGATGTTGTTCGAACGGAAGCCCGCTTTAAACAGCTTGGACGCGGCATAACCTTCCCATATAGTCCACTGACCAGAACCGAACATACCGATCGATTCCGGCCCTTTTTCTTTCAGGGCGGTTTTGaa                                                                           <  1:3003/149‑1 (MQ=255)
                                                                 aTACAGTGACGCGCGTTCGGGTCGATGTTGTTCGAACGGAAGCCCGCTTTAAACAGCTTGGACGCGGCATAACCTTCCCATATAGTCCACTGACCAGAACCGAACATACCGATCGATTCCGGCCCTTTTTCTTTCAGGGCGGTTTTGaa                                                                           <  1:356978/149‑1 (MQ=255)
                                                                 aTACAGTGACGCGCGTTCGGGTCGATGTTGTTCGAACGGAAGCCCGCTTTAAACAGCTTGGACGCGGCATAACCTTCCCATATAGTCCACTGACCAGAACCGAACATACCGATCGATTCCGGCCCTTTTTCTTTCAGGGCGGTTTTGaa                                                                           <  4:688352/149‑1 (MQ=255)
                                                                  tACAGTGACGCGCGTTCGGGTCGATGTTGTTCGAACGGAAGCCCGCTTTAAACAGCTTGGACGCGGCATAACCTTCCCATATAGTCCACTGACCAGAACCGAACATACCGATCGATTCCGGCCCTTTTTCTTTCAGGGCGGTTTTGAAt                                                                          <  4:758003/149‑1 (MQ=255)
                                                                    cAGTGACGCGCGTTCGGGTCGATGTTGTTCGAACGGAAGCCCGCTTTAAACAGCTTGGACGCGGCATAACCTTCCCATATAGTCCACTGACCAGAACCGAACATACCGATCGATTCCGGCCCTTTTTCTTTCAGGGCGGTTTTGAAttt                                                                        <  2:126266/149‑1 (MQ=255)
                                                                     aGTGACGCGCGTTCGGGTCGATGTTGTTCGAACGGAAGCCCGCTTTAAACAGCTTGGACGCGGCATAACCTTCCCATATAGTCCACTGACCAGAACCGAACATACCGATCGATTCCGGCCCTTTTTCTTTCAGGGCGGTTTTGAATTtc                                                                       <  1:692435/149‑1 (MQ=255)
                                                                      gTGACGCGCGTTCGGGTCGATGTTGTTCGAACGGAAGCCCGCTTTAAACAGCTTGGACGCGGCATAACCTTCCCATATAGTCCACTGACCAGAACCGAACATACCGATCGATTCCGGCCCTTTTTCTTTCAGGGCGGTTTTGAATTtct                                                                      <  3:17427/149‑1 (MQ=255)
                                                                             gcgTTCGGGTCGATGTTGTTCGAACGGAAGCCCGCTTTAAACAGCTTGGACGCGGCATAACCTTCCCATATAGTCCACTGACCAGAACCGAACATACCGATCGATTCCGGCCCTTTTTCTTTCAGGGCGGTTTTGAATTTCTCTTCCAt                                                               >  3:562878/1‑149 (MQ=255)
                                                                                 tCGGGTCGATGTTGTTCGAACGGAAGCCCGCTTTAAACAGCTTGGACGCGGCATAACCTTCCCATATAGTCCACTGACCAGAACCGAACATACCGATCGATTCCGGCCCTTTTTCTTTCAGGGCGGTTTTGAATTTCTCTTCCATcaca                                                           >  3:154442/1‑149 (MQ=255)
                                                                                       cGATGTTGTTCGAACGGAAGCCCGCTTTAAACAGCTTGGACGCGGCATAACCTTCCCATATAGTCCACTGACCAGAACCGAACATACCGATCGATTCCGGCCCTTTTTCTTTCAGGGCGGTTTTGAATTTCTCTTCCATCACATCGAAg                                                     <  2:537177/149‑1 (MQ=255)
                                                                                       cGATGTTGTTCGAACGGAAGCCCGCTTTAAACAGCTTGGACGCGGCATAACCTTCCCATATAGTCCACTGACCAGAACCGAACATACCGATCGATTCCGGCCCTTTTTCTTTCAGGGCGGTTTTGAATTTCTCTTCCATCACATCGAAg                                                     <  1:839521/149‑1 (MQ=255)
                                                                                        gATGTTGTTCGAACGGAAGCCCGCTTTAAACAGCTTGGACGCGGCATAACCTTCCCATATAGTCCACTGACCAGAACCGAACATACCGATCGATTCCGGCCCTTTTTCTTTCAGGGCGGTTTTGAATTTCTCTTCCATCACATCGAAgg                                                    >  1:111055/1‑149 (MQ=255)
                                                                                        gATGTTGTTCGAACGGAAGCCCGCTTTAAACAGCTTGGACGCGGCATAACCTTCCCATATAGTCCACTGACCAGAACCGAACATACCGATCGATTCCGGCCCTTATTCTTTCAGGGAGGTTTTGAATTTCTCTTCCATCACATCGAAgg                                                    >  2:815006/1‑149 (MQ=255)
                                                                                          tgttgtTCGAACGGAAGCCCGCTTTAAACAGCTTGGACGCGGCATAACCTTCCCATATAGTCCACTGACCAGAACCGAACATACCGATCGATTCCGGCCCTTTTTCTTTCAGGGCGGTTTTGAATTTCTCTTCCATCACATCGAAGGcc                                                  <  2:674838/149‑1 (MQ=255)
                                                                                           gttgttCGAACGGAAGCCCGCTTTAAACAGCTTGGACGCGGCATAACCTTCCCATATAGTCCACTGACCAGAACCGAACATACCGATCGATTCCGGCCCTTTTTCTTTCAGGGCGGTTTTGAATTTCTCTTCCATCACATCGAAGGCCt                                                 >  3:496120/1‑149 (MQ=255)
                                                                                              gttCGAACGGAAGCCCGCTTTAAACAGCTTGGACGCGGCATAACCTTCCCATATAGTCCACTGACCAGAACCGAACATACCGATCGATTCCGGCCCTTTTTCTTTCAGGGCGGTTTTGAATTTCTCTTCCATCACATCGAAGGCCTGAt                                              >  4:337500/1‑149 (MQ=255)
                                                                                               ttCGAACGGAAGCCCGCTTTAAACAGCTTGGACGCGGCATAACCTTCCCATATAGTCCACTGACCAGAACCGAACATACCGATCGATTCCGGCCCTTTTTCTTTCAGGGCGGTTTTGAATTTCTCTTCCATCACATCGAAGGCCTGATc                                             >  1:377944/1‑149 (MQ=255)
                                                                                                  gAACGGAAGCCCGCTTTAAACAGCTTGGACGCGGCATAACCTTCCCATATAGTCCACTGACCAGAACCGAACATACCGATCGATTCCGGCCCTTTTTCTTTCAGGGCGGTTTTGaa                                                                           >  1:315253/1‑116 (MQ=255)
                                                                                                  gAACGGAAGCCCGCTTTAAACAGCTTGGACGCGGCATAACCTTCCCATATAGTCCACTGACCAGAACCGAACATACCGATCGATTCCGGCCCTTTTTCTTTCAGGGCGGTTTTGaa                                                                           <  2:315253/116‑1 (MQ=255)
                                                                                                  gAACGGAAGCCCGCTTTAAACAGCTTGGACGCGGCATAACCTTCCCATATAGTCCACTGACCAGAACCGAACATACCGATCGATTCCGGCCCTTTTTCTTTCAGGGCGGTTTTGAATTTCTCTTCCATCACATCGAAGGCCTGATCCCa                                          <  2:227367/149‑1 (MQ=255)
                                                                                                    aCGGAAGCCCGCTTTAAACAGCTTGGACGCGGCATAACCTTCCCATATAGTCCACTGACCAGAACCGAACATACCGATCGATTCCGGCCCTTTTTCTTTCAGGGCGGTTTTGAATTTCTCTTCCATCACATCGAAGGCCTGATCCCAgg                                        >  1:11168/1‑149 (MQ=255)
                                                                                                      ggAAGCCCGCTTTAAACAGCTTGGACGCGGCATAACCTTCCCATATAGTCCACTGACCAGAACCGAACATACCGATCGATTCCGGCCCTTTTTCTTTCAGGGCGGTTTTGAATTTCTCTTCCATCACATCGAAGGCCTGATCCCAGGTg                                      <  1:248007/149‑1 (MQ=255)
                                                                                                           cccGCTTTAAACAGCTTGGACGCGGCATAACCTTCCCATATAGTCCACTGACCAGAACCGAACATACCGATCGATTCCGGCCCTTTTTCTTTCAGGGCGGTTTTGAATTTCTCTTCCATCACATCGAAGGCCTGATCCCAg                                         <  4:323014/141‑1 (MQ=255)
                                                                                                           cccGCTTTAAACAGCTTGGACGCGGCATAACCTTCCCATATAGTCCACTGACCAGAACCGAACATACCGATCGATTCCGGCCCTTTTTCTTTCAGGGCGGTTTTGAATTTCTCTTCCATCACATCGAAGGCCTGATCCCAg                                         >  3:323014/1‑141 (MQ=255)
                                                                                                           cccGCTTTAAACAGCTTGGACGCGGCATAACCTTCCCATATAGTCCACTGACCAGAACCGAACATACCGATCGATTCCGGCCCTTTTTCTTTCAGGGCGGTTTTGAATTTCTCTTCCATCACATCGAAGGCCTGATCCCAGGTGATTgg                                 <  1:459064/149‑1 (MQ=255)
                                                                                                            ccGCTTTAAACAGCTTGGACGCGGCATAACCTTCCCATATAGTCCACTGACCAGaa                                                                                                                             <  4:564116/56‑1 (MQ=255)
                                                                                                            ccGCTTTAAACAGCTTGGACGCGGCATAACCTTCCCATATAGTCCACTGACCAGaa                                                                                                                             >  3:564116/1‑56 (MQ=255)
                                                                                                            ccGCTTTAAACAGCTTGGACGCGGCATAACCTTCCCATATAGTCCACTGACCAGAACCGAACATACCGATCGATTCCGGCCCTTTTTCTTTCAGGGCGGTTTTGAATTTCTCTTCCATCACATCGAAGGCCTGATCCCAGGTGATTggg                                <  2:111055/149‑1 (MQ=255)
                                                                                                            ccGCTTTAAACAGCTTGGACGCGGCATAACCTTCCCATATAGTCCACTGACCAGAACCGAACATACCGATCGATTCCGGCCCTTTTTCTTTCAGGGCGGTTTTGAATTTCTCTTCCATCACATCGAAGGCCTGATCCCAGGTGATTggg                                >  1:809451/1‑149 (MQ=255)
                                                                                                            ccGCTTTAAACAGCTTGGACGCGGCATAACCTTCCCATATAGTCCACTGACCAGAACCGAACATACCGATCGATTCCGGCCCTTTTTCTTTCAGGGCGGTTTTGAATTTCTCTTCCATCACATCGAAGGCCTGATCCCAGGTGATTggg                                <  2:811887/149‑1 (MQ=255)
                                                                                                            ccGCTTTAAACAGCTTGGACGCGGCATAACCTTCCCATATAGTCCACTGACCAGAACCGAACATACCGATCGATTCCGGCCCTTTTTCTTTCAGGGCGGTTTTGAATTTCTCTTCCATCACATCGAAGGCCTGATCCCAGGTGATTggg                                >  4:583723/1‑149 (MQ=255)
                                                                                                             cGCTTTAAACAGCTTGGACGCGGCATAACCTTCCCATATAGTCCACTGACCAGAACCGAACATACCGATCGATTCCGGCCCTTTTTCTTTCAGGGCGGTTTTGAATTTCTCTTCCATCACATCGAAGGCCTGATCCCAGGTGATTgggg                               <  2:482255/149‑1 (MQ=255)
                                                                                                              gCTTTAAACAGCTTGGACGCGGCATAACCTTCCCATATAGTCCACTGACCAGAACCGAACATACCGATCGATTCCGGCCCTTTTTCTTTCAGGGCGGTTTTGAATTTCTCTTCCATCACATCGAAGGCCTGATCCCAGGTGATTGGGGt                              >  1:402298/1‑149 (MQ=255)
                                                                                                              gCTTTAAACAGCTTGGACGCGGCATAACCTTCCCATATAGTCCACTGACCAGAACCGAACATACCGATCGATTCCGGCCCTTTTTCTTTCAGGGCGGTTTTGAATTTCTCTTCCATCACATCGAAGGCCTGATCCCAGGTGATTGGGGt                              >  1:226788/1‑149 (MQ=255)
                                                                                                               cTTTAAACAGCTTGGACGCGGCATAACCTTCCCATATAGTCCACTGACCAGAACCGAACATACCGATCGATTCCGGCCCTTTTTCTTTCAGGGCGGTTTTGAATTTCTCTTCCATCACATCg                                                        >  1:517611/1‑122 (MQ=255)
                                                                                                               cTTTAAACAGCTTGGACGCGGCATAACCTTCCCATATAGTCCACTGACCAGAACCGAACATACCGATCGATTCCGGCCCTTTTTCTTTCAGGGCGGTTTTGAATTTCTCTTCCATCACATCg                                                        <  2:517611/122‑1 (MQ=255)
                                                                                                                 ttAAACAGCTTGGACGCGGCATAACCTTCCCATATAGTCCACTGACCAGAACCGAACATACCGATCGATTCCGGCCCTTTTTCTTTCAGGGCGGTTTTGAATTTCTCTTCCATCACATCGAAGGCCTGATCCCAGGTGATTGGGGTaaa                           <  3:822140/149‑1 (MQ=255)
                                                                                                                    aaCAGCTTGGACGCGGCATAACCTTCCCATATAGTCCACTGACCAGAACCGAACATACCGATCGATTCCGGCCCTTTTTCTTTCAGGGCGGTTTTGAATTTCTCTTCCATCACATCGAAGGCCTGATCCCAGGTGATTggg                                <  2:532412/141‑1 (MQ=255)
                                                                                                                    aaCAGCTTGGACGCGGCATAACCTTCCCATATAGTCCACTGACCAGAACCGAACATACCGATCGATTCCGGCCCTTTTTCTTTCAGGGCGGTTTTGAATTTCTCTTCCATCACATCGAAGGCCTGATCCCAGGTGATTggg                                >  1:532412/1‑141 (MQ=255)
                                                                                                                        gCTTGGACGCGGCATAACCTTCCCATATAGTCCACTGACCAGAACCGAACATACCGATCGATTCCGGCCCtttt                                                                                               <  4:172881/74‑1 (MQ=255)
                                                                                                                        gCTTGGACGCGGCATAACCTTCCCATATAGTCCACTGACCAGAACCGAACATACCGATCGATTCCGGCCCtttt                                                                                               >  3:172881/1‑74 (MQ=255)
                                                                                                                         cTTGGACGCGGCATAACCTTCCCATATAGTCCACTGACCAGAACCGAACATACCGATCGATTCCGGCCCTTTTTCTTTCAGGGCGGTTTTGAATTTCTCTTCCATCACATCGAAGGCCTGATCCCAGGTGATTGGGGTAAATTCGCctt                   <  1:656976/149‑1 (MQ=255)
                                                                                                                          ttGGACGCGGCATAACCTTCCCATATAGTCCACTGACCAGAACCGAACATACCGATCGATTCCGGCCCTTTTTCTTTCAGGGCGGTTTTGAATTTCTCTTCCATCACATCGAAGGCCTGATCCCAGGTGATTGGGGTAAATTCGCcttc                  >  4:254415/1‑149 (MQ=255)
                                                                                                                          ttGGACGCGGCATAACCTTCCCATATAGTCCACTGACCAGAACCGAACATACCGATCGATTCCGGCCCTTTTTCTTTCAGGGCGGTTTTGAATTTCTCTTCCATCACATCGAAGGCCTGATCCCAGGTGATTGGGGTAAATTCGCcttc                  >  2:227244/1‑149 (MQ=255)
                                                                                                                             gACGCGGCATAACCTTCCCATATAGTCCACTGACCAGAGCCGAACATACCGATCGATTCCGGCCCTTTTTCTTTCAGGGCGGTTTTGAATTTCTCTTCCATCACATCGAAGGCCTGATCCCAGGTGATTGGGGTAAATTCGCCTTCttt               <  1:284742/149‑1 (MQ=255)
                                                                                                                              aCGCGGCATAACCTTCCCATATAGTCCACTGACCAGAACCGAACATACCGATCGATTCCGGCCCTTTTTCTTTCAGGGCGGTTTTGAATTTCTCTTCCATCACATCGAAGGCCTGATCCCAGGTGATTGGGGTAAATTCGCCTTCTTTg              <  3:254415/149‑1 (MQ=255)
                                                                                                                               cgcgGCATAACCTTCCCATATAGTCCACTGACCAGAACCGAACATACCGATCGATTCCGGCCCTTTTTCTTTCAGGGCGGTTTTGAATTTCTCTTCCATCACATCGAAGGCCTGATCCCAGGTGATTGGGGTAAATTCGCCTTCTTTGt             <  2:831781/149‑1 (MQ=255)
                                                                                                                                gcgGCATAACCTTCCCATATAGTCCACTGACCAGAACCGAACATACCGATCGATTCCGGCCCTTTTTCTTTCAGGGCGGTTTTGAATTTCTCTTCCATCACATCGAAg                                                     >  1:390098/1‑108 (MQ=255)
                                                                                                                                gcgGCATAACCTTCCCATATAGTCCACTGACCAGAACCGAACATACCGATCGATTCCGGCCCTTTTTCTTTCAGGGCGGTTTTGAATTTCTCTTCCATCACATCGAAg                                                     <  2:390098/108‑1 (MQ=255)
                                                                                                                                 cgGCATAACCTTCCCATATAGTCCACTGACCAGAACCGAACATACCGATCGATTCCGGCCCTTTTTCTTTCAGGGCGGTTTTGAATTTCTCTTCCATCACATCGAAGGCCTGATCCCAGGTGATTGGGGTAAATTCGCCTTCTTTGTCa           <  4:562878/149‑1 (MQ=255)
                                                                                                                                   gCATAACCTTCCCATATAGTCCACTGACCAGAACCGAACATACCGATCGATTCCGGCCCTTTTTCTTTCAGGGCGGTTTTGAATTTCTCTTCCATCACATCGAAGGCCTGATCCCAGGTGATTGGGGTAAATTCGCCTTCTTTGTCata         <  3:90540/149‑1 (MQ=255)
                                                                                                                                     aTAACCTTCCCATATAGTCCACTGACCAGAACCGAACATACCGATCGATTCCGGCCCTTTTTCTTTCAGGGCGGTTTTGAATTTCTCTTCCATCACATCGAAGGCCTGATCCCAGGTGATTGGGGTAAATTCGCCTTCTTTGTCATAtt       >  4:110957/1‑149 (MQ=255)
                                                                                                                                       aaCCTTCCCATATAGTCCACTGACCAGAACCGAACATACCGATCGATTCCGGCCCTTTTTCTTTCAGGGCGGTTTTGAATTTCTCTTCCATCACATCGAAGGCCTGATCCCAGGTGATTGGGGTAAATTCGCCTTCTTTGTCATATTTa     >  2:175550/1‑149 (MQ=255)
                                                                                                                                        aCCTTCCCATATAGTCCACTGACCAGAACCGAACATACCGATCGATTCCGGCCCTTTTTCTTTCAGGGCGGTTTTGAATTTCTCTTCCATCACATCGAAGGCCTGATCCCAGGTGATTGGGGTAAATTCGCCTTCTTTGTCATATTTAc    <  2:619256/149‑1 (MQ=255)
                                                                                                                                          cTTCCCATATAGTCCACTGACCAGAACCGAACATACCGATCGATTCCGGCCCTTTTTCTTTCAGGGCGGTTTTGAATTTCTCTTCCATCACATCGAAGGCCTGATCCCAGGTGATTGGGGTAAATTCGCCTTCTTTGTCATATTTACCg  >  3:590695/1‑149 (MQ=255)
                                                                                                                                          cTTCCCATATAGTCCACTGACCAGAACCGAACATACCGATCGATTCCGGCCCTTTTTCTTTCAGGGCGGTTTTGAATTTCTCTTCCATCACATCGAAGGCCTGATCCCAGGTGATTGGGGTAAATTCGCCTTCTTTGTCATATTTACCg  >  4:852399/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                             
CGTCATAGCAGCCCATCGGCTCATCCATACCAAAGGTACGCATAAAGCCAACTACTGCCGACGCCATACAGTGACGCGCGTTCGGGTCGATGTTGTTCGAACGGAAGCCCGCTTTAAACAGCTTGGACGCGGCATAACCTTCCCAGATAGTCCACTGACCAGAACCGAACATACCGATCGATTCCGGCCCTTTTTCTTTCAGGGCGGTTTTGAATTTCTCTTCCATCACATCGAAGGCCTGATCCCAGGTGATTGGGGTAAATTCGCCTTCTTTGTCATATTTACCG  >  NC_000913/2302150‑2302436

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: