BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,379,376	C→A	72.2%	G423V (GGG→GTG)	menF ←	isochorismate synthase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,379,376	0	C	A	72.2%	209.2 / 112.0	128	G423V (GGG→GTG)	menF	isochorismate synthase 2
Reads supporting (aligned to +/- strand): ref base C (19/17); new base A (53/39); total (72/56)
Fisher's exact test for biased strand distribution p-value = 6.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 130 total aligned reads displayed.

GTCGGTTAAATGCGCTTACTGACATGAGTTTGTCCGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCCGCGCCAGCATATAATCGCACGACATTGCCGCTAATTTTTGCTGAGCGCAGGGAAACGCAGAATTCGCTTTGTTGTAATGAGAGA  >  NC_000913/2379235‑2379522

gTCGGTTAAATGCGCTTACTGACATGAGTTTGTCCGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGc                                                                                                                                             >  3:113546/1‑149 (MQ=255)

   ggTTAAATGCGCTTACTGACATGAGTTTGTCCGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCACTGCCGCttt                                                                                                                                          <  4:31225/149‑1 (MQ=255)

   ggTTAAATGCGCTTACTGACATGAGTTTGTCCGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCACTGCCGCttt                                                                                                                                          <  1:722984/149‑1 (MQ=255)

   ggTTAAATGCGCTTACTGACATGAGTTTGTCCGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCACTGCCGCttt                                                                                                                                          <  2:831889/149‑1 (MQ=255)

   ggTTAAATGCGCTTACTGACATGAGTTTGTCCGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCACTGCCGCttt                                                                                                                                          >  4:1665/1‑149 (MQ=255)

   ggTTAAATGCGCTTACTGACATGAGTTTGTCCGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCACTGCCGCttt                                                                                                                                          <  2:264223/149‑1 (MQ=255)

    gTTAAATGCGCTTACTGACATGAGTTTGTCCGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCACTGCCGCTttg                                                                                                                                         >  2:225576/1‑149 (MQ=255)

     ttAAATGCGCTTACTGACATGAGTTTGTCCGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCACTGCCGCTttgt                                                                                                                                        >  4:545834/1‑149 (MQ=255)

      tAAATGCGCTTACTGACATGAGTTTGTCCGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCACTGCCGCTttgtt                                                                                                                                       <  1:132825/149‑1 (MQ=255)

      tAAATGCGCTTACTGACATGAGTTTGTCCGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCACTGCCGCTttgtt                                                                                                                                       <  3:204845/149‑1 (MQ=255)

         aTGCGCTTACTGACATGAGTTTGTCCGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCACTGCCGCTTTGTTGTc                                                                                                                                    <  3:97670/149‑1 (MQ=255)

         aTGCGCTTACTGACATGAGTTTGTCCGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCACTGCCGCTTTGTTGTc                                                                                                                                    >  3:65707/1‑149 (MQ=255)

             gcTTACTGACATGAGTTTGTCCGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGAtt                                                                                                                                >  4:251605/1‑149 (MQ=255)

             gcTTACTGACATGAGTTTGTCCGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCACTGCCGCTTTGTTGTCGAtt                                                                                                                                >  1:392125/1‑149 (MQ=255)

             gcTTACTGACATGAGTTTGTCCGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCACTGCCGCTTTGTTGTCGAtt                                                                                                                                >  4:350455/1‑149 (MQ=255)

             gcTTACTGAAATGTGTTTGTCCGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAATAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGAtt                                                                                                                                >  4:670241/1‑149 (MQ=255)

               ttACTGACATGAGTTTGTCCGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTc                                                                                                                              >  2:937771/1‑149 (MQ=255)

                   tGACATGAGTTTGTCCGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGc                                                                                                                          >  2:4332/1‑149 (MQ=255)

                   tGACATGAGTTTGTCCGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGc                                                                                                                          >  2:497361/1‑149 (MQ=255)

                   tGACATGAGTTTGTCCGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGATGTCGATTTCCTGc                                                                                                                          <  1:497361/149‑1 (MQ=255)

                   tGACAAGAGATTGTCCGATATCAATATTGCGGCTAAGTATAATGAGGTACAAAAATAGATTATTGATATGAATCGGTAATGATGAGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCACTGCCGCTTTGTGGTCGATTTCCTGc                                                                                                                          <  2:148685/149‑1 (MQ=255)

                     aCATGAGTTTGTCCGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAAGAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCACTGCCGCTTTGTTGTCGATTTCCTGCCa                                                                                                                        >  2:277709/1‑149 (MQ=255)

                     aCATGAGTTTGTCCGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCa                                                                                                                        >  2:222640/1‑149 (MQ=255)

                     aCATGAGTTTGTCCGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCACTGCCGCTTTGTTGTCGATTTCCTGCCa                                                                                                                        >  1:208110/1‑149 (MQ=255)

                       aTGAGTTTGTCCGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCACTGCCGCTTTGTTGTCGATTTCCTGCCAct                                                                                                                      >  4:230400/1‑149 (MQ=255)

                        tGAGTTTGTCCGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCActc                                                                                                                     >  3:331594/1‑149 (MQ=255)

                        tGAGTTTGTCCGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCACTGCCGCTTTGTTGTCGATTTCCTGCCActc                                                                                                                     >  4:130812/1‑149 (MQ=255)

                        tGAGTTTGTCCGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCACTGCCGCTTTGTTGTCGATTTCCTGCCActc                                                                                                                     >  2:197639/1‑149 (MQ=255)

                            tttGTCCGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCACTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGc                                                                                                                 <  2:208110/149‑1 (MQ=255)

                              tGTCCGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCACTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTc                                                                                                               <  3:674383/149‑1 (MQ=255)

                                 ccGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCggg                                                                                                            <  2:905808/149‑1 (MQ=255)

                                  cGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAATAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCACTGCCGATTTGTTGTCGATTTCCTGCCACTCTTGCTCgggg                                                                                                           >  1:766720/1‑149 (MQ=255)

                                  cGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCACTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCgggg                                                                                                           <  4:280603/149‑1 (MQ=255)

                                    gTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTGTAATAAGGTACGCAGCACTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTc                                                                                                         <  1:480260/149‑1 (MQ=255)

                                    gTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCACTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTc                                                                                                         <  1:225576/149‑1 (MQ=255)

                                       tCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGa                                                                                                      >  2:355291/1‑149 (MQ=255)

                                       tCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCACTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGa                                                                                                      >  2:709684/1‑149 (MQ=255)

                                        cAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGaa                                                                                                     >  4:402600/1‑149 (MQ=255)

                                        cAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCACTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGaa                                                                                                     <  2:257430/149‑1 (MQ=255)

                                         aaTATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAAc                                                                                                    >  1:149739/1‑149 (MQ=255)

                                            atTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCACTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCAc                                                                                                 <  4:866387/149‑1 (MQ=255)

                                               gCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCACTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGa                                                                                              <  1:36473/149‑1 (MQ=255)

                                                  gCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCACTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACaa                                                                                           <  3:130812/149‑1 (MQ=255)

                                                   cTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCACTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAAt                                                                                          <  3:1665/149‑1 (MQ=255)

                                                   cTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCACTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAAt                                                                                          >  1:705291/1‑149 (MQ=255)

                                                    tAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCACTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATg                                                                                         >  2:606513/1‑149 (MQ=255)

                                                      aGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGcc                                                                                       <  4:822318/149‑1 (MQ=255)

                                                           aaGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCACTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCCgcgc                                                                                  <  2:392125/149‑1 (MQ=255)

                                                                 cTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCACTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCCGCGCCAGCat                                                                            >  4:333740/1‑149 (MQ=255)

                                                                 cTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCACTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCCGCGCCAGCat                                                                            >  3:455621/1‑149 (MQ=255)

                                                                  tACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCACTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCCGCGCCAGCata                                                                           >  2:834423/1‑149 (MQ=255)

                                                                       aaaTAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCACTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCCGCGCCAGCATATAATc                                                                      >  1:867952/1‑149 (MQ=255)

                                                                          tAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCCGCGCCAGCATATAATCGCa                                                                   <  1:4332/149‑1 (MQ=255)

                                                                                  tGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCCGCGCCAGCATATAATCGCACGACATTg                                                           <  3:402600/149‑1 (MQ=255)

                                                                                    atatGAATCGGTAATGATGCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCCGCGCCAGCATATAATCGCACGACATTGcc                                                         >  2:662720/1‑149 (MQ=255)

                                                                                    atatGAATCGGTAATGATGCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCACTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCCGCGCCAGCATATAATCGCACGACATTGcc                                                         <  4:164242/149‑1 (MQ=255)

                                                                                      atGAATCGGTAATGATGCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCACTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCCGCGCCAGCATATAATCGCACGACATTGCCGc                                                       >  1:42852/1‑149 (MQ=255)

                                                                                          aTCGGTAATGATGCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCActct                                                                                                                    >  1:767586/1‑84 (MQ=255)

                                                                                                aaTGATGCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCACTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCCGCGCCAGCATATAATCGCACGACATTGCCGCTAATTTTTGc                                             >  2:376524/1‑149 (MQ=255)

                                                                                                   gatgCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCACTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCCGCGCCAGCATATAATCGCACGACATTGCCGCTAATTTTTGCTGa                                          >  1:143575/1‑149 (MQ=255)

                                                                                                     tgCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCCGCGCCAGCATATAATCGCACGACATTGCCGCTAATTTTTGCTGAgc                                        >  2:163476/1‑149 (MQ=255)

                                                                                                     tgCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCACTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCCGCGCCAGCATATAATCGCACGACATTGCCGCTAATTTTTGCTGAgc                                        >  4:636640/1‑149 (MQ=255)

                                                                                                        gACTCATTACTATTCCATTTGTAATAAAGTACGCAGCACTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCCGCGCCAGCATATAATCGCACGACATTGCCGCTAATTTTTGCTGAGCGCa                                     <  2:705291/149‑1 (MQ=255)

                                                                                                          cTCATTACTATTCCATTTGTAATAAAGTACGCAGCACTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCCGCGCCAGCATATAATCGCACGACATTGCCGCTAATTTTTGCTGAGCGCAgg                                   <  2:87422/149‑1 (MQ=255)

                                                                                                           tCATTACTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCCGCGCCAGCATATAATCGCACGACATTGCCGCTAATTTTTGCTGAGCGCAggg                                  >  3:315544/1‑149 (MQ=255)

                                                                                                           tCATTACTATTCCATTTGTAATAAAGTACGCAGCACTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCCGCGCCAGCATATAATCGCACGACATTGCCGCTAATTTTTGCTGAGCGCAggg                                  >  2:478390/1‑149 (MQ=255)

                                                                                                              ttACTATTCCATTTGTAATAAAGTACGCAGCACTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCCGCGCCAGCATATAATCGCACGACATTGCCGCTAATTTTTGCTGAGCGCAGGGaaa                               >  3:548379/1‑149 (MQ=255)

                                                                                                                  tATTCCATTTGTAATAAAGTACGCAGCACTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCCGCGCCAGCATATAATCGCACGACATTGCCGCTAATTTTTGCTGAGCGCAGGGAAACGCa                           >  2:239676/1‑149 (MQ=255)

                                                                                                                    ttCCATTTGTAATAAAGTACGCAGAACTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCCGCGCCAGCATATAATCGCACGACATTGCCGCTAATTTTTGCTGAGCGCAGGGAAACGCAGa                         >  3:770089/1‑149 (MQ=255)

                                                                                                                     tCCATTTGTAATAAAGTACGCAGCACTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCCGCGCCAGCATATAATCGCACGACATTGCCGCTAATTTTTGCTGAGCGCAGGGAAACGCAGaa                        >  2:235858/1‑149 (MQ=255)

                                                                                                                       cATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTc                                                                                                                              <  2:483734/45‑1 (MQ=255)

                                                                                                                       cATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTc                                                                                                                              >  1:483734/1‑45 (MQ=255)

                                                                                                                       cATTTGTAATAAAGTACGCAGCACTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCCGCGCCAGCATATAATCGCACGACATTGCCGCTAATTTTTGCTGAGCGCAGGGAAACGCAGAAtt                      <  1:834423/149‑1 (MQ=255)

                                                                                                                        aTTTGTAATAAAGTACGCAGCACTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACTGACAATGCCCGCGCCAGCATATAATGGAACGACATTGCCGCTAATTTTTGCTGAGCGCAGGGGAATGCAGAATTc                     >  3:781750/1‑149 (MQ=255)

                                                                                                                        aTTTGTAATAAAGTACGCAGCACTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCCGCGCCAGCATATAATCGCACGACATTGCCGCTAATTTTTGCTGAGCGCAGGGAAACGCAGAATTc                     >  4:562740/1‑149 (MQ=255)

                                                                                                                         tttGTAATAAAGTACGCAGCACTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCCGCGCCAGCATATAATCGCACGACATTGCCGCTAATTTTTGCTGAGCGCAGGGaa                                <  4:905778/137‑1 (MQ=255)

                                                                                                                         tttGTAATAAAGTACGCAGCACTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCCGCGCCAGCATATAATCGCACGACATTGCCGCTAATTTTTGCTGAGCGCAGGGaa                                >  3:905778/1‑137 (MQ=255)

                                                                                                                         tttGTAATAAAGTACGCAGCACTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCCGCGCCAGCATATAATCGCACGACATTGCCGCTAATTTTTGCTGAGCGCAGGGAAACGCAGAATTCg                    >  2:452061/1‑149 (MQ=255)

                                                                                                                          ttGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCCGCGCCAGCATATAATCGCACGACATTGCCGCTAATTTTTGCTGAGCGCAGGGAAACGCAGAATTCGc                   <  1:662720/149‑1 (MQ=255)

                                                                                                                            gTAATAAAGTACGCAGCACTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCCGCGCCAGCATATAATCGCACGACATTGCCGCTAATTTTTGCTGAGCGCAGGGAAACGCAGAATTCGCtt                 <  4:906817/149‑1 (MQ=255)

                                                                                                                              aataaAGTACGCAGCACTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCCGCGCCAGCATATAATCGCACGACATTGCCGCTAATTTTTGCTGAGCGCAGGGAAACGCAGAATTCGCTttg               >  1:400277/1‑149 (MQ=255)

                                                                                                                               ataaAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCCGCGCCAGCATATAATCGCACGACATTGCCGCTAATTTTTGCTGAGCGCAGGGAAACGCAGAATTCGCttt                <  4:317965/147‑1 (MQ=255)

                                                                                                                               ataaAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCCGCGCCAGCATATAATCGCACGACATTGCCGCTAATTTTTGCTGAGCGCAGGGAAACGCAGAATTCGCttt                >  3:317965/1‑147 (MQ=255)

                                                                                                                               ataaAGTACGCAGCACTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCCGCGCCAGCATATAATCGCACGACATTGCCGCTAATTTTTGCTGAGCGCAGGGAAACGCAGAATTCGCTttgt              >  1:747176/1‑149 (MQ=255)

                                                                                                                               ataaAGTACGCAGCACTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCCGCGCCAGCATATAATCGCACGACATTGCCGCTAATTTTTGCTGAGCGCAGGGAAACGCAGAATTCGCTttgt              <  4:501442/149‑1 (MQ=255)

                                                                                                                               ataaAGTACGCAGCACTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCCGCGCCAGCATATAATCGCACGACATTGCCGCTAATTTTTGCTGAGCGCAGGGAAACGCAGAATTCGCTttgt              >  2:733052/1‑149 (MQ=255)

                                                                                                                                taaAGTACGCAGCACTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCCGCGCCAGCATATAATCGCACGACATTGCCGCTAATTTTTGCTGAGCGCAGGGAAACGGAGAATTCGCTttgtt             >  2:195782/1‑149 (MQ=255)

                                                                                                                                  aaGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCCGCGCCAGCATATAATCGCACGACATTGCCGCTAATTTTTGCTGAGCGCAGGGAAACGCAGAATTCGCTTtgttgt           >  3:47905/1‑149 (MQ=255)

                                                                                                                                    gTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCCGCGCCAGCATATAATCGCACGACATTGCCGCTAATTTTTGCTGAGCGCAGGGAAACGCAGAATTCGCTTTGTTGTaa         <  4:315544/149‑1 (MQ=255)

                                                                                                                                    gTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCCGCGCCAGCATATAATCGCACGACATTGCCGCTAATTTTTGCTGAGCGCAGGGAAACGCAGAATTCGCTTTGTTGTaa         <  4:113546/149‑1 (MQ=255)

                                                                                                                                    gTACGCAGCACTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCCGCGCCAGCATATAATCGCACGACATTGCCGCTAATTTTTGCTGAGCGCAGGGAAACGCAGAATTCGCTTTGTTGTaa         <  4:902560/149‑1 (MQ=255)

                                                                                                                                       cGCAGCACTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCCGCGCCAGCATATAATCGCACGACATTGCCGCTAATTTTTGCTGAGCGCAGGGAAACGCAGAATTCGCTTTGTTGTAATga      <  1:57897/149‑1 (MQ=255)

                                                                                                                                       cGCAGCACTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCCGCGCCAGCATATAATCGCACGACATTGCCGCTAATTTTTGCTGAGCGCAGGGAAACGCAGAATTCGCTTTGTTGTAATga      <  1:204362/149‑1 (MQ=255)

                                                                                                                                       cGCAGCACTGCCGCTTTGTTGTCGATTACCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCCGCGCCAGCATATAATCGCACGACATTGCCGCTAATTTTTGCTGAGCGCAGGGAAACGCAGAATTCGCTTTGTTGTAATga      <  3:636640/149‑1 (MQ=255)

                                                                                                                                        gcagcaCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCCGCGCCAGCATATAATCGCACGACATTGCCGCTAATTTTTGCTGAGCGCAGGGAAACGCAGAATTCGCTTTGTTGTAATgag     >  3:72673/1‑149 (MQ=255)

                                                                                                                                        gcagcaCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCCGCGCCAGCATATAATCGCACGACATTGCCGCTAATTTTTGCTGAGCGCAGGGAAACGCAGAATTCGCTTTGTTGTAATgag     >  4:216756/1‑149 (MQ=255)

                                                                                                                                          aGCACTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCCGCGCCAGCATATAATCGCACGACATTGCCGCTAATTTTTGCTGAGCGCAGGGAAACGCAGAATTCGCTTTGTTGTAATgagag   <  3:32655/149‑1 (MQ=255)

                                                                                                                                          aGCACTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCCGCGCCAGCATATAATCGCACGACATTGCCGCTAATTTTTGCTGAGCGCAGGGAAACGCAGAATTCGCTTTGTTGTAATgaga    <  2:14936/148‑1 (MQ=255)

                                                                                                                                          aGCACTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCCGCGCCAGCATATAATCGCACGACATTGCCGCTAATTTTTGCTGAGCGCAGGGAAACGCAGAATTCGCTTTGTTGTAATgaga    >  1:14936/1‑148 (MQ=255)

                                                                                                                                           gCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCCGCGCCAGCATATAATCGCACGACATTGCCGCTAATTTTTGCTGAGCGCAGGGAAACGCAGAATTCGCTTTGTTGTAATgagaga  <  3:251605/149‑1 (MQ=255)

GTCGGTTAAATGCGCTTACTGACATGAGTTTGTCCGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCCGCGCCAGCATATAATCGCACGACATTGCCGCTAATTTTTGCTGAGCGCAGGGAAACGCAGAATTCGCTTTGTTGTAATGAGAGA  >  NC_000913/2379235‑2379522

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`