| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,419,395 | C→G | 7.0% | V132L (GTG→CTG) | yfcE ← | phosphodiesterase activity on bis‑pNPP |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,419,395 | 0 | C | G | 7.0% | 455.5 / 21.1 | 129 | V132L (GTG→CTG) | yfcE | phosphodiesterase activity on bis‑pNPP |
| Reads supporting (aligned to +/- strand): ref base C (56/64); new base G (6/3); total (62/67) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.11e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.39e-02 | |||||||||||
Only 100 of 129 total aligned reads displayed.
GACCTGCGCAATGATACTTTGATCATTGAGTGCGATTACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGACGTTGTTTTTCCAGTAATACCTGTTGCCACGGCGCGGTTATCGGGAAATGCAG > NC_000913/2419249‑2419542 | gACCTGCGCAATGATACTTTGATCATTGAGTGCGATTACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTg < 1:813477/149‑1 (MQ=255) gcgcAATGATACTTTGATCATTGAGTGCGATTACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGa < 2:362055/149‑1 (MQ=255) gcgcAATGATACTTTGATCATTGAGTGCGATTACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGa > 4:906717/1‑149 (MQ=255) cgcAATGATACTTTGATCATTGAGTGCGATTACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGAt > 2:388977/1‑149 (MQ=255) cAATGATACTTTGATCATTGAGTGCGATTACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATgg < 3:711684/149‑1 (MQ=255) cAATGATACTTTGATCATTGAGTGCGATTACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATgg < 3:358295/149‑1 (MQ=255) aaTGATACTTTGATCATTGAGTGCGATTACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATggg > 1:96416/1‑149 (MQ=255) aTGATACTTTGATCATTGAGTGCGATTACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGt < 1:429300/149‑1 (MQ=255) aTACTTTGATCATTGAGTGCGATTACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATggg > 4:911252/1‑145 (MQ=255) aTACTTTGATCATTGAGTGCGATTACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATg < 1:642507/149‑1 (MQ=255) tACTTTGATCATTGAGTGCGATTACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGa < 3:273668/149‑1 (MQ=255) cTTTGATCATTGAGTGCGATTACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGTGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGAcc < 1:602309/149‑1 (MQ=255) tttGATCATTGAGTGCGATTACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCg > 4:583353/1‑149 (MQ=255) tCATTGAGTGCGATTACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTacac > 1:641112/1‑149 (MQ=255) tCATTGAGTGCGATTACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTacac > 4:65184/1‑149 (MQ=255) ttGAGTGCGATTACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAg > 2:335736/1‑149 (MQ=255) tGAGTGCGATTACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGc < 4:837655/149‑1 (MQ=255) aGTGCGATTACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGcac > 3:365868/1‑149 (MQ=255) gCGATTACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATc < 3:421714/149‑1 (MQ=255) aTTACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGtt < 1:660481/149‑1 (MQ=255) ttACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTc < 3:235194/149‑1 (MQ=255) tACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCt < 3:4287/149‑1 (MQ=255) cacTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTgg < 3:583353/149‑1 (MQ=255) acTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGt > 2:332728/1‑149 (MQ=255) acTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGt > 2:55920/1‑149 (MQ=255) aaGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTgg < 3:904829/145‑1 (MQ=255) aGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTaa < 1:645764/149‑1 (MQ=255) gTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTaaa > 3:355714/1‑149 (MQ=255) tACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAg > 1:52163/1‑149 (MQ=255) cGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAgca < 4:896149/149‑1 (MQ=255) gTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAgcag > 2:661084/1‑149 (MQ=255) gTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAgcag > 4:558033/1‑149 (MQ=255) gTCATAATCCAGCATGCCATAACTCGCCGGATTGCCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAgcag > 1:266695/1‑149 (MQ=255) tCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAgcagg < 1:335736/149‑1 (MQ=255) aTTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAgcaggca > 3:111739/1‑149 (MQ=255) ttATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAgcaggcag > 2:431862/1‑149 (MQ=255) tATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGa < 1:55920/149‑1 (MQ=255) aTCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGCTCTGGTTTAAAGCAGGCAGAt > 3:444242/1‑149 (MQ=255) tCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGAtt > 1:50100/1‑149 (MQ=255) cAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGAtttt > 1:703230/1‑149 (MQ=255) aGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTc > 1:401626/1‑149 (MQ=255) aGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTc < 4:227033/149‑1 (MQ=255) aGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTc < 3:246578/149‑1 (MQ=255) aGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTc < 1:661221/149‑1 (MQ=255) cATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCg > 1:568144/1‑149 (MQ=255) aTGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCgg < 4:397071/149‑1 (MQ=255) aTGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCgg < 4:656055/149‑1 (MQ=255) gCCCTAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGc < 3:380076/149‑1 (MQ=255) ccATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGcc > 1:168365/1‑149 (MQ=255) aCTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCaaaaa < 4:16043/149‑1 (MQ=255) tCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGa < 4:641537/149‑1 (MQ=255) cGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGAt < 4:381027/149‑1 (MQ=255) gCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATg > 1:470549/1‑149 (MQ=255) ccGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGa < 2:96416/149‑1 (MQ=255) ggATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGAcc > 1:231427/1‑149 (MQ=255) gATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCa > 3:138030/1‑149 (MQ=255) gATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCa > 1:935037/1‑149 (MQ=255) ttACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATg > 4:158063/1‑149 (MQ=255) tACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGc < 2:26433/149‑1 (MQ=255) ccGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGa > 2:764999/1‑138 (MQ=255) ccGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGa < 1:764999/138‑1 (MQ=255) ccGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGt > 4:28794/1‑149 (MQ=255) cGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTc > 3:438993/1‑149 (MQ=255) cGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTc > 1:596455/1‑149 (MQ=255) gAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGa < 2:856564/149‑1 (MQ=255) cTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGACgttg > 1:929568/1‑149 (MQ=255) tCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGACgttgt > 3:192626/1‑149 (MQ=255) tCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGACgttgt < 3:152275/149‑1 (MQ=255) tCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGACgttgt > 2:450313/1‑149 (MQ=255) aCCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGTTGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAATGATGACCATGCGTCAAAAACAGACGTTGttt < 4:566178/149‑1 (MQ=255) ccGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGACGTTGtttt > 3:550520/1‑149 (MQ=255) aGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGACGTTGTTTTTcc < 2:641600/149‑1 (MQ=255) gCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGACGTTGTTTTTCCa < 1:711270/149‑1 (MQ=255) gCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGACGTTGTTTTTCCa < 2:568144/149‑1 (MQ=255) gggTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGACGTTGTTTTTCCAGTaa < 2:875779/149‑1 (MQ=255) gTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGACGTTGTTTTTCCAGTAATACCTGtt < 4:355714/149‑1 (MQ=255) tGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGAc < 4:917060/124‑1 (MQ=255) tGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGAc > 3:917060/1‑124 (MQ=255) aaTCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGACGTTGTTTTTCCAGTAATACCTGTTGCCACg > 3:675999/1‑149 (MQ=255) aTCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGACGTTGTTTTTCCAGTAATACCTGTTGCCACgg < 2:703230/149‑1 (MQ=255) ctcCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGACGTTGTTTTTCCAGTAATACCTGTTGCCACGgcg > 1:336359/1‑149 (MQ=255) tcCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGACGTTGTTTTTCCAGTAATACCTGTTGCCACGgcgc < 3:158063/149‑1 (MQ=255) ccccccGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGACGTTGTTTTTCCAGTAATACCTGTTGCCACGGcgcg < 2:336359/149‑1 (MQ=255) cccGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGACGTTGTTTTTCCAGTAATACCTGt > 3:99036/1‑134 (MQ=255) cGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGACGTTGTTTTTCCAGTAATACCTGTTGCCACGGCGCGGTTAt < 1:388977/149‑1 (MQ=255) cTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGACGTTGTTTTTCCAGTAATACCTGTTGCCACGGCGCGGTTATCg < 4:365868/149‑1 (MQ=255) cTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGACGTTGTTTTTCCAGTAATACCTGTTGCCACGGCGCGGTTATCg < 2:747313/149‑1 (MQ=255) ccatCTGCCAGTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGACGTTGTTTTTCCAGTAATACCTGTTGCCACGGCGCGGTTATCgg > 4:613809/4‑149 (MQ=255) ccatCTGCCAGTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGACGTTGTTTTTCCAGTAATACCTGTTGCCACGGCGCGGTTATCgg > 1:441632/4‑149 (MQ=255) gTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGACGTTGTTTTTCCAGTAATACCTGTTGCCACGGCGCGGTTATCggg < 1:151276/149‑1 (MQ=255) catCTGCCAGTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGACGTTGTTTTTCCAGTAATACCTGTTGc > 1:848083/3‑131 (MQ=255) catCTGCCAGTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGACGTTGTTTTTCCAGTAATACCTGTTGc < 2:848083/129‑1 (MQ=255) catCTGCCAGTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGACGTTGTTTTTCCAGTAATACCTGTTGCCACGGCGCGGTTATCggg > 4:790656/3‑149 (MQ=255) atCTGCCAGTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGACGTTGTTTTTCCAGTAATACCTGTTGCCACGGCGCGGTTATCg < 2:362403/145‑1 (MQ=255) tGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGACGTTGTTTTTCCAGTAATACCTGTTGCCACGGCGCGGTTATCGGGaaaa > 3:80223/1‑148 (MQ=255) gCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGACGTTGTTTTTCCAGTAATACCTGTTGCCACGGCGCGGTTATCGGGAAATg < 2:641112/149‑1 (MQ=255) ccACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGACGTTGTTTTTCCAGTAATACCTGTTGCCACGGCGCGGTTATCGGGAAATgc < 4:438993/149‑1 (MQ=255) cACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGACGTTGTTTTTCCAGTAATACCTGTTGCCACGGCGCGGTTATCGGGAAATgca > 2:932318/1‑149 (MQ=255) cACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGACGTTGTTTTTCCAGTAATACCTGTTGCCACGGCGCGGTTATCGGGAAATgca < 1:332728/149‑1 (MQ=255) aCTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGACGTTGTTTTTCCAGTAATACCTGTTGCCACGGCGCGGTTATCGGGAAATgcag > 2:75223/1‑149 (MQ=255) | GACCTGCGCAATGATACTTTGATCATTGAGTGCGATTACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGACGTTGTTTTTCCAGTAATACCTGTTGCCACGGCGCGGTTATCGGGAAATGCAG > NC_000913/2419249‑2419542 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |