Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 2,425,971 | C→A | 7.1% | intergenic (‑55/+35) | hisQ ← / ← hisJ | histidine ABC transporter permease/histidine ABC transporter periplasmic binding protein |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 2,425,971 | 0 | C | A | 7.1% | 344.3 / 12.4 | 99 | intergenic (‑55/+35) | hisQ/hisJ | histidine ABC transporter permease/histidine ABC transporter periplasmic binding protein |
Reads supporting (aligned to +/- strand): ref base C (46/46); new base A (4/3); total (50/49) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.62e-01 |
Only 100 of 103 total aligned reads displayed.
AACCGATGATTACAGCGAGCACTACAGAGCTGATAGCCAGCTCCAGCGTGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAGCGCGCATTTCGGCAAAGGCTTTGTTCAGTGCTTCGCGCAGTTCGTTAT > NC_000913/2425823‑2426115 | aaCCGATGATTACAGCGAGCACTACAGAGCTGATAGCCAGCTCCAGCGTGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTAc < 1:142041/149‑1 (MQ=255) aaCCGATGATTACAGCGAGCACTACAGAGCTGATAGCCAGCTCCAGCGTGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTAc > 2:782916/1‑149 (MQ=255) aaCCGATGATTACAGCGAGCACTACAGAGCTGATAGCCAGCTCCAGCGTGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTAc < 2:392159/149‑1 (MQ=255) aCCGATGATTACAGCGAGCACTACAGAGCTGATAGCCAGCTCCAGCGTGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTAcc < 3:167860/149‑1 (MQ=255) gatgatTACAGCGAGCACTACAGAGCTGATAGCCAGCTCCAGCGTGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGa > 1:698075/1‑149 (MQ=255) atgatTACAGCGAGCACTACAGAGCTGATAGCCAGCTCCAGCGTGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGaa < 3:795313/149‑1 (MQ=255) atgatTACAGCGAGCACTACAGAGCTGATAGCCAGCTCCAGCGTGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGaa > 2:892309/1‑149 (MQ=255) atgatTACAGCGAGCACTACAGAGCTGATAGCCAGCTCCAGCGTGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGaa < 3:644124/149‑1 (MQ=255) gatTACAGCGAGCACTACAGAGCTGATAGCCAGCTCCAGCGTGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTTCGGGTAACCGGAgg < 1:791881/149‑1 (MQ=255) gatTACAGCGAGCACTACAGAGCTGATAGCCAGCTCCAGCGTGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAgg < 1:349434/149‑1 (MQ=255) ttACAGCGAGCACTACAGAGCTGATAGCCAGCTCCAGCGTGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGa > 3:576221/1‑149 (MQ=255) ttACAGCGAGCACTACAGAGCTGATAGCCAGCTCCAGCGTGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGa < 4:544516/149‑1 (MQ=255) aGCGAGCACTACAGAGCTGATAGCCAGCTCCAGCGTGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGACGGGAGCgg < 3:854127/149‑1 (MQ=255) aGCGAGCACTACAGAGCTGATAGCCAGCTCCAGCGTGACGAGCGCACCCTGTAAAATAAAACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCgg < 1:572117/149‑1 (MQ=255) gAGCACTACAGAGCTGATAGCCAGCTCCAGCGTGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGct < 2:698075/149‑1 (MQ=255) gAGCACTACAGAGCTGATAGCCAGCTCCAGCGTGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGct < 3:146452/149‑1 (MQ=255) aGCACTACAGAGCTGATAGCCAGCTCCAGCGTGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGctt < 4:104547/149‑1 (MQ=255) gCACTACAGAGCTGATAGCCAGCTCCAGCGTGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGcttt < 2:268729/149‑1 (MQ=255) aCTACAGAGCTGATAGCCAGCTCCAGCGTGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGctttct > 4:622917/1‑149 (MQ=255) aCTACAGAGCTGATAGCCAGCTCCAGCGTGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGctttct > 4:161653/1‑149 (MQ=255) tACAGAGCTGATAGCCAGCTCCAGCGTGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGctttcttt > 2:505050/1‑149 (MQ=255) tACAGAGCTGATAGCCAGCTCCAGCGTGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGctttcttt < 4:34785/149‑1 (MQ=255) tACAGAGCTGATAGCCAGCTCCAGCGTGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGctttcttt > 3:380375/1‑149 (MQ=255) aCAGAGCTGATAGCCAGCTCCAGCGTGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCtttctttc < 1:95048/149‑1 (MQ=255) cTGATAGCCAGCTCCAGCGTGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGAt < 1:505050/149‑1 (MQ=255) cTGATAGCCAGCTCCAGCGTGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGAt > 1:226794/1‑149 (MQ=255) tGATAGCCAGCTCCAGCGTGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATg > 4:182745/1‑149 (MQ=255) aTAGCCAGCTCCAGCGTGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGa < 4:222613/126‑1 (MQ=255) aTAGCCAGCTCCAGCGTGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGa > 3:222613/1‑126 (MQ=255) cAGCTCCAGCGTGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAGCATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTa < 4:469407/149‑1 (MQ=255) aGCTCCAGCGTGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAg > 3:173861/1‑149 (MQ=255) cTCCAGCGTGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGcc < 4:151114/149‑1 (MQ=255) tCCAGCGTGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGcca > 3:707142/1‑149 (MQ=255) aGCGTGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGccacca > 4:314549/1‑149 (MQ=255) gCGTGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCAt > 1:507511/1‑149 (MQ=255) gTGACGAGCGCACCCTGTAAAATAACACCAGAAAACCCGTACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATaa < 1:688651/149‑1 (MQ=255) gcgcACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACa < 2:47112/145‑1 (MQ=255) gcgcACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACa > 1:47112/1‑145 (MQ=255) ccTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGa < 1:216780/149‑1 (MQ=255) tAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTa < 3:432479/149‑1 (MQ=255) aTAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACttt > 2:286596/1‑149 (MQ=255) tAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGaa > 4:216365/1‑85 (MQ=255) tAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGaa < 3:216365/85‑1 (MQ=255) acacCTGAAAAGCCATACAACATGCTGCCTGTCCAGTCGTGTGTGGTGAATTACCGTCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGCGCTTTCTTTCACCGGTGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTc < 4:570299/149‑1 (MQ=255) acacCTGAAAACCCATACAACATGCTGCCTCTCCTGACGTGTGTGGTGAATTACCGCCTCTTCTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAGCAGCAAAATCGAAGTACtttttg > 2:252411/1‑148 (MQ=255) acCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGc < 1:268140/149‑1 (MQ=255) tGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTaa > 4:297724/1‑149 (MQ=255) tGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTaa > 1:23492/1‑149 (MQ=255) gAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAAt < 4:197984/149‑1 (MQ=255) aaaaCCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAAtt > 2:631202/1‑149 (MQ=255) ccATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCg > 1:243729/1‑149 (MQ=255) aTACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTa > 1:116162/1‑149 (MQ=255) acaTGCTGCCTGTCCTGTCGTGTGCGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAACTTCTCGTAAGTAc > 2:198483/1‑149 (MQ=255) aTGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCg > 4:774543/1‑149 (MQ=255) tgcCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAg > 4:265350/1‑149 (MQ=255) cctgtcctgtCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAgcg > 1:698527/1‑149 (MQ=255) cctgtcctgtCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAgcg > 2:858568/1‑149 (MQ=255) gtcctgtcGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAgcgcgc < 3:161653/149‑1 (MQ=255) gtcctgtcGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAgcgcgc < 3:622917/149‑1 (MQ=255) gtcGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAGCGCGCATTTc > 2:42149/1‑149 (MQ=255) gtcGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAGCGCGCATTTc < 2:532493/149‑1 (MQ=255) gtcGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAGCGCGCATTTc < 4:173861/149‑1 (MQ=255) tgtgGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAGCGCGCATTTCGGCaaa < 3:774543/149‑1 (MQ=255) gtggtgAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAGCGCGCATTTCGGCAAAg < 1:286596/149‑1 (MQ=255) gtggtgAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAGCGCGCATTTCGGCAAAg < 2:69988/149‑1 (MQ=255) tggtgAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAGCGCGCATTTCGGCAAAgg > 4:354580/1‑149 (MQ=255) tgAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAGCGCGCATTTCGGCAAAGGCtt > 1:287320/1‑149 (MQ=255) gAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAGCGCGCATTTCGGCAAAGGCttt > 4:766378/1‑149 (MQ=255) ttACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTaa > 3:104740/1‑104 (MQ=255) ttACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTaa < 4:104740/104‑1 (MQ=255) ttACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAGCGCGCATTTCGGCAAAGGCTTTGtt < 3:187026/149‑1 (MQ=255) ttACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAGCGCGCATTTCGGCAAAGGCTTTGtt > 3:871645/1‑149 (MQ=255) tACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAGCGCGCATTTCGGCAAAGGCTTTGTTc < 4:197318/149‑1 (MQ=255) ccGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAGCGCGCATTTCGGCAAAGGCTTTGTTCAg < 4:380375/149‑1 (MQ=255) ccGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAGCGCGCATTTCGGCAAAGGCTTTGTTCAg < 3:312218/149‑1 (MQ=255) ccGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAGCGCGCATTTCGGCAAAGGCTTTGTTCAg > 4:192223/1‑149 (MQ=255) ccTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAGCGCGCATTTCGGCAAAGGCTTTGTTCAGTGc > 2:494950/1‑149 (MQ=255) tctTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAGCGCGCATTTCGGCAAAGGCTTTGTTCAGTGCtt > 4:5885/1‑149 (MQ=255) tctTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAGCGCGCATTTCGGCAAAGGCTTTGTTCAGTGCtt < 3:88356/149‑1 (MQ=255) ctTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAGCGCGCATTTCGGCAAAGGCTTTGTTCAGTGCTTc < 1:902432/149‑1 (MQ=255) ctTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAGCGCGCATTTCGGCAAAGGCTTTGTTCAGTGCTTc < 3:297724/149‑1 (MQ=255) ttGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAGCGCGCATTTCGGCAAAGGCTTTGTTCAGTGCTTcg > 2:753732/1‑149 (MQ=255) ccgctcccttcGGGTAACCGGAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAGCGCGCATTTCGGCAAAGGCTTTGTTCAGTGCTTcgc > 1:333227/4‑149 (MQ=255) ctcccttcGGGTAACCGGAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAGCGCGCATTTCGGCAAAGGCTTTGTTCAGt > 3:700072/1‑139 (MQ=255) ctcccttcGGGTAACCGGAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAGCGCGCATTTCGGCAAAGGCTTTGTTCAGt < 4:700072/139‑1 (MQ=255) ctccctccGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAGCGCGCATTTCGGCAAAGGCTTTGTTCAGTGCTTCGCGCa < 1:892309/149‑1 (MQ=255) tcccttcGGGTAACCGGAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAGCGCGCATTTCGGCAAAGGCTTTGTTCAGTGCTTCGCGCAg > 4:20755/1‑149 (MQ=255) tcccttcGGGTAACCGGAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAGCGCGCATTTCGGCAAAGGCTTTGTTCAGTGCTTCGCGCAg > 4:334027/1‑149 (MQ=255) tccctccGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAGCGCGCATTTCGGCAAAGGCTTTGTTCAGTGCTTCGCGCAg > 3:185165/1‑149 (MQ=255) cctccGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAGCGCGCATTTCg > 2:898815/1‑118 (MQ=255) cctccGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAGCGCGCATTTCg < 1:898815/118‑1 (MQ=255) cctccGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAGCGCGCATTTCGGCAAAGGCTTTGTTCAGTGCTTCGCGCAGtt > 1:474034/1‑149 (MQ=255) cctccGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAGCGCGCATTTCGGCAAAGGCTTTGTTCAGTGCTTCGCGCAGtt > 2:752336/1‑149 (MQ=255) cttcGGGTAACCGGAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAGCGCGCATTTCGGCAAAGGCTTTGTTCAGTGCTTCGCGCAGTTc < 2:333227/146‑1 (MQ=255) ctccGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAGCGCGCATTTCGGCAAAGGCTTTGTTCAGTGCTTCGCGCAGTTc > 3:40987/1‑149 (MQ=255) ctccGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAGCGCGCATTTCGGCAAAGGCTTTGTTCAGTGCTTCGCGCAGTTc > 3:518985/1‑149 (MQ=255) tccGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAGCGCGCATTTCGGCAAAGGCTTTGTTCAGTGCTTCGCGCAGTTCg < 2:70949/149‑1 (MQ=255) ccGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAGCGCGCATTTCGGCAAAGGCTTTGTTCAGTGCTTCGCGCAGTTCGt < 3:182745/149‑1 (MQ=255) cGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAGCGCGCATTTCGGCAAAGGCTTTGTTCAGTGCTTCGCGCAGTTCGtt < 3:314549/149‑1 (MQ=255) gTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAGCGCGCATTTCGGCAAAGGCTTTGTTCAGTGCTTCGCGCAGTTCGTTAt < 4:707142/149‑1 (MQ=255) | AACCGATGATTACAGCGAGCACTACAGAGCTGATAGCCAGCTCCAGCGTGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAGCGCGCATTTCGGCAAAGGCTTTGTTCAGTGCTTCGCGCAGTTCGTTAT > NC_000913/2425823‑2426115 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |