Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,594,337 C→A 76.6% G508V (GGT→GTT)  tmcA ← elongator methionine tRNA (ac4C34) acetyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,594,3370CA76.6% 189.7 / 72.5 98G508V (GGT→GTT) tmcAelongator methionine tRNA (ac4C34) acetyltransferase
Reads supporting (aligned to +/- strand):  ref base C (11/12);  new base A (38/37);  total (49/49)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.41e-01

GCTTGCGCATCGCGACGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAACCGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAGGTCTTGCGTATATTGCAAAGCACCAGCAATAAGTTGCCGCCCTGTGCCTTCCCGCTGACGAGCCGGATGAACTGCTATC  >  NC_000913/2594199‑2594480
                                                                                                                                          |                                                                                                                                               
gCTTGCGCATCGCGACGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAACCGCAGCGTTg                                                                                                                                       >  3:186453/1‑149 (MQ=255)
  ttGCGCATCGCGACGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAACCGCAGCGTTGcc                                                                                                                                     >  4:349902/1‑149 (MQ=255)
  ttGCGCATCGCGACGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAAACGCAGCGTTGcc                                                                                                                                     >  2:73725/1‑149 (MQ=255)
  ttGCGCATCGCGACGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAAACGCAGCGTTGcc                                                                                                                                     >  4:92671/1‑149 (MQ=255)
  ttGCGCATCGCGACGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAAACGCAGCGTTGcc                                                                                                                                     >  1:910876/1‑149 (MQ=255)
        aTCGCGACGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAACCGCAGCGTTGCCAGAAAc                                                                                                                               <  2:312888/149‑1 (MQ=255)
          cgcgACGTAAACGGTAATGCTCGCGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAAACGCAGCGTTGCCAGAAACGc                                                                                                                             <  1:802527/149‑1 (MQ=255)
          cgcgACGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAAACGCAGCGTTGCCAGAAACGc                                                                                                                             <  1:73725/149‑1 (MQ=255)
          cgcgACGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAACACAAAAACGCAGCGTTGCCAGAAACGc                                                                                                                             <  3:317897/149‑1 (MQ=255)
          cgcgACGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTAGCTTCCCGATGATTACCCATCCGCACCAGCACAACACCGCAGCGTTGCCAGAAACGc                                                                                                                             <  4:137123/149‑1 (MQ=255)
               cGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAAACGCAGCGTTGCCAGAAACGCCATaa                                                                                                                        <  4:502963/149‑1 (MQ=255)
               cGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAAACGCAGCGTTGCCAGAAACGCCATaa                                                                                                                        <  2:531915/149‑1 (MQ=255)
               cGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAAACGCAGCGTTGCCAGAAACGCCATaa                                                                                                                        <  2:320399/149‑1 (MQ=255)
                gTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAACCGCAGCGTTGCCAGAAACGCCATAAc                                                                                                                       >  1:761744/1‑149 (MQ=255)
                 tAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAAACGCAGCGTTGc                                                                                                                                      >  2:853520/1‑133 (MQ=255)
                 tAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAAACGCAGCGTTGc                                                                                                                                      <  1:853520/133‑1 (MQ=255)
                 tAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAAACGCAGCGTTGCCAGAAACGCCATAACt                                                                                                                      >  1:209859/1‑149 (MQ=255)
                   aaCGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAAACGCAGCGTTGCCAGAAACGCCATAACTcc                                                                                                                    >  2:488149/1‑149 (MQ=255)
                    aCGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCTGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAACCGCAGCGTTGCCAGAAACGCCATAACTccc                                                                                                                   <  3:770358/149‑1 (MQ=255)
                    aCGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAAACGCAGCGTTGCCAGAAACGCCATAACTccc                                                                                                                   <  2:139021/149‑1 (MQ=255)
                            gCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAACCGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTaa                                                                                                           <  4:186453/149‑1 (MQ=255)
                              tCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAACCGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAAcc                                                                                                         <  3:865648/149‑1 (MQ=255)
                               cACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAAACGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCa                                                                                                        >  4:826465/1‑149 (MQ=255)
                                   ttCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAAACGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAAc                                                                                                    >  2:49370/1‑149 (MQ=255)
                                           gcTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAAACGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGaa                                                                                            >  3:911864/1‑149 (MQ=255)
                                              gTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAAACGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGAAAGa                                                                                         >  4:100649/1‑149 (MQ=255)
                                              gTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAAACGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGAAAGa                                                                                         >  2:425120/1‑149 (MQ=255)
                                               tttACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCTCACCAGCACAAAAACGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGaaa                                                                                           >  3:117985/1‑146 (MQ=255)
                                               tttACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAAACGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGAAAGAt                                                                                        >  3:917841/1‑149 (MQ=255)
                                               tttACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACAGCTGCTGGCTTCCCGCTGATTACCCATCCGCACCAGCACAAAAACGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGaaa                                                                                           <  4:117985/146‑1 (MQ=255)
                                                 tACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCGACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAAACGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGAAAGATAg                                                                                      <  1:728591/149‑1 (MQ=255)
                                                   cccGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAACCGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGAAAGATAGTc                                                                                    >  2:244386/1‑149 (MQ=255)
                                                   cccGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAAACGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGAAAGATAGTc                                                                                    <  1:425120/149‑1 (MQ=255)
                                                     cGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAACCGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGAAAGATAGTCGa                                                                                  >  4:629564/1‑149 (MQ=255)
                                                     cGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAAACGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGAAAGATAGTCGa                                                                                  <  3:826465/149‑1 (MQ=255)
                                                      gCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAACCGCAGCGTTGCAAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAg                                                                                 <  3:753803/149‑1 (MQ=255)
                                                         tCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAAACGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAGGTc                                                                              >  3:863159/1‑149 (MQ=255)
                                                           aCTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAAACGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAGGTCtt                                                                            >  1:761454/1‑149 (MQ=255)
                                                             tCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAACCGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGAAAGATAGTCGa                                                                                  >  2:857717/1‑141 (MQ=255)
                                                             tCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAACCGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGAAAGATAGTCGa                                                                                  <  1:857717/141‑1 (MQ=255)
                                                             tCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAAACGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCa                                                                                                 >  2:373110/1‑126 (MQ=255)
                                                             tCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAAACGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCa                                                                                                 <  1:373110/126‑1 (MQ=255)
                                                             tCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAAACGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAGGTCTTGc                                                                          >  3:332650/1‑149 (MQ=255)
                                                             tCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAAACGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAGGTCTTGc                                                                          >  2:172905/1‑149 (MQ=255)
                                                               aTCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAAACGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAGGTCTTGCGt                                                                        >  1:39721/1‑149 (MQ=255)
                                                               aTCAGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCTCACCAGCACAAAAACGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAGGTCTTGCGt                                                                        >  4:789172/1‑149 (MQ=255)
                                                                tCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAAACGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAGGTCTTGCGta                                                                       >  1:332710/1‑149 (MQ=255)
                                                                   gTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAACACAAAAACGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAGGTCTTGCGTatat                                                                    >  2:448292/1‑149 (MQ=255)
                                                                     aaCAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAAACGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCa                                                                                                        >  2:682140/1‑111 (MQ=255)
                                                                     aaCAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAAACGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCa                                                                                                        <  1:682140/111‑1 (MQ=255)
                                                                        aGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAAACGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAGGTCTTGCGTATATTGCaa                                                               >  4:823643/1‑149 (MQ=255)
                                                                         gcgcCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAAACGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAGGTCTTGCGTATATTGCaaa                                                              <  4:917841/149‑1 (MQ=255)
                                                                         gcgcCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAAACGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAGGTCTTGCGTATATTGCaaa                                                              <  2:475105/149‑1 (MQ=255)
                                                                             cATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAAACGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAGGTCTTGCGTATATTGCAAAGCAc                                                          >  2:392578/1‑149 (MQ=255)
                                                                               tCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAACCGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAGGTCTTGCGTATATTGCAAAGCACCa                                                        <  1:178359/149‑1 (MQ=255)
                                                                               tCGCCGTAGACCACCCGTTGCTGGCTTGCAGATGATTATCCCTACGCACCAGCACAAAACCGCAGCGTTGCCAGAGACTTCATATCTCCGCGGTGTACACAAAACTCCACGAAGGATAGTCGACGTATTGCGTATACTGCAAAGCACCa                                                        <  2:372687/149‑1 (MQ=255)
                                                                                    gTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAAACGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAGGTCTTGCGTATATTGCAAAGCACCAGCAAt                                                   <  1:732329/149‑1 (MQ=255)
                                                                                      ataGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAAACGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAGGTCTTGCGTATATTGCAAAGCACCAGCAATaa                                                 <  1:392578/149‑1 (MQ=255)
                                                                                      ataGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAAACGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAGGTCTTGCGTATATTGCAAAGCACCAGCAATaa                                                 <  4:863159/149‑1 (MQ=255)
                                                                                         gCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAAACGCAGCGTTGCCAg                                                                                                                                   >  3:129676/1‑64 (MQ=255)
                                                                                         gCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAAACGCAGCGTTGCCAg                                                                                                                                   <  4:129676/64‑1 (MQ=255)
                                                                                          cAACCGCTGCTGGCTTCCCGATGATTACCCATCCACACCAGCACAAAAACGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGAAAGATAGTCGATGTCTTGCGTATATTGCAAAGCACCAGCAATAAGTTg                                             >  3:819080/1‑149 (MQ=255)
                                                                                                 tgctgGCTTCCCGATGATTACCCATCCGCACCAGCACAAAAACGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAGGTCTTGCGTATATTGCAAAGCACCAGCAATAAGTTGCCGCCCt                                      <  2:209859/149‑1 (MQ=255)
                                                                                                   ctgGCTTCCCGATGATAACCCATCCGCACCAGCACAAAAACGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAGGTCTTGCGTATATTGCAAAGCACCAGCAATAAGTTGCCGCCCtgt                                    <  3:789172/149‑1 (MQ=255)
                                                                                                    tgGCTTCCCGATGATTACCCATCCGCACCAGCACAAAAACGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAGGTCTTGCGTATATTGCAAAGCACCAGCAATAAGTTGCCGCCCtgtg                                   <  1:353064/149‑1 (MQ=255)
                                                                                                     ggCTTCCCGATGATTACCCATCCGCACCAGCACAAAAACGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAGGTCTTGCGTATATTGCAAAGCACCAGCAATAAGTTGCCGCCCTGTGc                                  <  2:111886/149‑1 (MQ=255)
                                                                                                      gCTTCCCGATGATTACCCATCCGCACCAGCACAAAAACGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAGGTCTTGCGTATATTGCAAAGCACCAGCAATAAGTTGCCGCCCTGTGcc                                 >  4:224583/1‑149 (MQ=255)
                                                                                                          cccGATGATTACCCATCCGCACCAGCACAAAAACGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTGACCGAAAGATAGTCGAGGTCTTGCGTATATTGCAAAGCACCAGCAATAAGTTGCCGCCCTGTGCCTTcc                             >  4:51286/1‑149 (MQ=255)
                                                                                                           ccGATGATTACCCATCCGCACCAGCACAAGAACGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAGGTCTTGCGTATATTGCAAAGCACCAGCAATAAGTTGCCGCCCTGTGCCTTccc                            >  2:169534/1‑149 (MQ=255)
                                                                                                            cGATGATTACCCATCCGCACCAGCACAACACGGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAGGTCTTGCGTATATTGCAAAGCACCa                                                        >  4:567013/1‑120 (MQ=255)
                                                                                                            cGATGATTACCCATCCGCACCAGCACAAAACCGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAGGTCTTGCGTATATTGCAAAGCACCa                                                        >  2:92803/1‑120 (MQ=255)
                                                                                                            cGATGATTACCCATCCGCACCAGCACAAAACCGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAGGTCTTGCGTATATTGCAAAGCACCa                                                        <  3:567013/120‑1 (MQ=255)
                                                                                                            cGATGATTACCCATCCGCACCAGCACAAAACCGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAGGTCTTGCGTATATTGCAAAGCACCa                                                        <  1:92803/120‑1 (MQ=255)
                                                                                                            cGATGATTACCCATCCGCACCAGCACAAAAACGAAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAACCTCACAGAAAGATAGTCGAGGTCTTGCGTATATTGAAAAGCACCAGCAATAAGTTTACGCCCTATGCCTTCCCg                           >  4:571265/1‑149 (MQ=255)
                                                                                                             gatgatTACCCATCCGCACCAGCACAAAACCGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAGGTCTTGCGTATATTGCAAAGCACCAGCAATAAGTTGCCGCCCTGTGCCTTCCCGc                          >  2:717570/1‑149 (MQ=255)
                                                                                                             gatgatTACCCATCCGCACCAGCACAAAAACGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAGGTCTTGCGTATATTGCAAAGCACCAGCAATAAGTTGCCGCCCTGTGCCTTCCCGc                          >  4:845894/1‑149 (MQ=255)
                                                                                                                gatTACCCATCCGCACCAGCACAAAAACGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAGGTCTTGCGTATATTGCAAAGCACCAGCAATAAGTTGCCGCCCTGTGCCTTCCCGCTGa                       <  2:332710/149‑1 (MQ=255)
                                                                                                                 atTACCCATCCGCACCAGCACAAAAACGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAGGTCTTGCGTATATTGCAAAGCACCAGCAATAAGTTGCCGCCCTGTGCCTTCCCGCTGAc                      <  3:823643/149‑1 (MQ=255)
                                                                                                                    aCCCATCCGCACCAGCACAAACCCTCAGCGTCGCCAAAAACGCCATAACTCCCCGGTGTAACCAACACTCACCGAAACATACTCGAGCTCTTGCGTAGATTGCAAACCACCAGCAATAAGTTGCCGCCCTGTGCCTTCCCGCTGACGAg                   <  2:769431/149‑1 (MQ=255)
                                                                                                                      ccATCCGCACCAGCACAAAACCGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAGGTCTTGCGTATATTGCAAAGCACCAGCAATAAGTTGCCGCCCTGTGCCTTCCCGCTGACGAGcc                 >  1:420370/1‑149 (MQ=255)
                                                                                                                      ccATCCGCACCAGCACAAAAACGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAGGTCTTGCGTATATTGCAAAGCACCAGCAATAAGTTGCCGCCCTGTGCCTTCCCGCTGACGAGcc                 >  2:855039/1‑149 (MQ=255)
                                                                                                                      ccATCCGCACCAGCACAAAAACGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAGGTCTTGCGTATATTGCAAAGCACCAGCAATAAGTTGCCGCCCTGTGCCTTCCCGCTGACGAGcc                 >  4:860164/1‑149 (MQ=255)
                                                                                                                         tCCGCACCAGCACAAAAACGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAGGTCTTGCGTATATTGCAAAGCACCAGCAATAAGTTGCCGCCCTGTGCCTTCCCGCTGACGAGCCGGa              <  3:845894/149‑1 (MQ=255)
                                                                                                                         tCCGCACCAGCACAAAAACGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAGATCTTGCGTATATTGCAAAGCACCAGCAATAAGTTGCCGCCCTGTGCCTTCCCGCTGAc                      >  2:712610/1‑141 (MQ=255)
                                                                                                                         tCCGCACCAGCACAAAAACGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAGATCTTGCGTATATTGCAAAGCACCAGCAATAAGTTGCCGCCCTGTGCCTTCCCGCTGAc                      <  1:712610/141‑1 (MQ=255)
                                                                                                                          ccGCACCAGCACAAAAACGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAGGTCTTGCGTATATTGCAAAGCACCAGCAATAAGTTGCCGCCCTGTGCCTTCCCGCTGACGAGCCGGAt             <  1:507218/149‑1 (MQ=255)
                                                                                                                           cGCACCAGCACAAAAACGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAGGTCTTGCGTATATTGCAAAGCACCAGCAATAAGTTGCCGCCCTGTGCCTTCCCGCTGACGAGCCGGATg            <  3:92671/149‑1 (MQ=255)
                                                                                                                             cACCAGCACAAAAACGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAGGTCTTGCGTATATTGCAAAGCACCAGCAATAAg                                                >  1:676856/1‑111 (MQ=255)
                                                                                                                             cACCAGCACAAAAACGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAGGTCTTGCGTATATTGCAAAGCACCAGCAATAAg                                                <  2:676856/111‑1 (MQ=255)
                                                                                                                             cACCAGCACAAAAACGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAGGTCTTGCGTATATTGCAAAGCACCAGCAATAAGTTGCCGCCCTGTGCCTTCCCGCTGACGAGCCGGATGaa          <  2:910876/149‑1 (MQ=255)
                                                                                                                              aCCAGCACAAAAACGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAGGTCTTGCGTATATTGCAAAGCACCAGCAATAAGTTGCCGCCCTGTGCCTTCCCGCTGACGAGCCGGATGAAc         <  4:819080/149‑1 (MQ=255)
                                                                                                                              aCCAGCACAAAAACGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAGGTCTTGCGTATATTGCAAAGCACCAGCAATAAGTTGCCGCCCTGTGCCTTCCCGCTGACGAGCCGGATGAAc         <  3:22663/149‑1 (MQ=255)
                                                                                                                              aCCAGCACAAAAACGCAGCGTTGCCAGAAACGCAATAACTCCCCGGTGTAACAAAAACTCACCGAAAGATAGTCGAGGTCTTGCGTATATTGCAAAGCACCAGCAATAAGTTGCCGCCCTGTGCCTTCCCGCTGACGAGCCGGATGAAc         <  1:627886/149‑1 (MQ=255)
                                                                                                                                 aGCACAAAAACGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAGGTCTTGCGTATATTGCAAAGCACCAGCAATAAGTTGCCGCCCTGTGCCTTCCCGCTGACGAGCCGGATGAACTGc      <  3:740001/149‑1 (MQ=255)
                                                                                                                                    acaAAACCGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAGGTCTTGCGTATATTGCAAAGCACCAGCAATAAGTTGCCGCCCTGTGCCTTCCCGCTGACGAGCCGGATGAACTGCTAt   >  2:443191/1‑149 (MQ=255)
                                                                                                                                     caAAAACGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAGGTCTTGCGTATATTGCAAAGCACCAGCAATAAGTTGCCGCCCTGTGCCTTCCCGCTGACGAGCCGGATGAACTGCTATc  >  2:536909/1‑149 (MQ=255)
                                                                                                                                      aaaaACGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAGGTCTTGCGTATATTGCAAAGCACCAGCAATaa                                                 >  1:922466/1‑101 (MQ=255)
                                                                                                                                      aaaaACGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAGGTCTTGCGTATATTGCAAAGCACCAGCAATaa                                                 <  2:922466/101‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                               
GCTTGCGCATCGCGACGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAACCGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAGGTCTTGCGTATATTGCAAAGCACCAGCAATAAGTTGCCGCCCTGTGCCTTCCCGCTGACGAGCCGGATGAACTGCTATC  >  NC_000913/2594199‑2594480

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: