Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 2,594,337 | C→A | 76.6% | G508V (GGT→GTT) | tmcA ← | elongator methionine tRNA (ac4C34) acetyltransferase |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 2,594,337 | 0 | C | A | 76.6% | 189.7 / 72.5 | 98 | G508V (GGT→GTT) | tmcA | elongator methionine tRNA (ac4C34) acetyltransferase |
Reads supporting (aligned to +/- strand): ref base C (11/12); new base A (38/37); total (49/49) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.41e-01 |
GCTTGCGCATCGCGACGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAACCGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAGGTCTTGCGTATATTGCAAAGCACCAGCAATAAGTTGCCGCCCTGTGCCTTCCCGCTGACGAGCCGGATGAACTGCTATC > NC_000913/2594199‑2594480 | gCTTGCGCATCGCGACGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAACCGCAGCGTTg > 3:186453/1‑149 (MQ=255) ttGCGCATCGCGACGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAACCGCAGCGTTGcc > 4:349902/1‑149 (MQ=255) ttGCGCATCGCGACGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAAACGCAGCGTTGcc > 2:73725/1‑149 (MQ=255) ttGCGCATCGCGACGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAAACGCAGCGTTGcc > 4:92671/1‑149 (MQ=255) ttGCGCATCGCGACGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAAACGCAGCGTTGcc > 1:910876/1‑149 (MQ=255) aTCGCGACGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAACCGCAGCGTTGCCAGAAAc < 2:312888/149‑1 (MQ=255) cgcgACGTAAACGGTAATGCTCGCGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAAACGCAGCGTTGCCAGAAACGc < 1:802527/149‑1 (MQ=255) cgcgACGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAAACGCAGCGTTGCCAGAAACGc < 1:73725/149‑1 (MQ=255) cgcgACGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAACACAAAAACGCAGCGTTGCCAGAAACGc < 3:317897/149‑1 (MQ=255) cgcgACGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTAGCTTCCCGATGATTACCCATCCGCACCAGCACAACACCGCAGCGTTGCCAGAAACGc < 4:137123/149‑1 (MQ=255) cGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAAACGCAGCGTTGCCAGAAACGCCATaa < 4:502963/149‑1 (MQ=255) cGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAAACGCAGCGTTGCCAGAAACGCCATaa < 2:531915/149‑1 (MQ=255) cGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAAACGCAGCGTTGCCAGAAACGCCATaa < 2:320399/149‑1 (MQ=255) gTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAACCGCAGCGTTGCCAGAAACGCCATAAc > 1:761744/1‑149 (MQ=255) tAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAAACGCAGCGTTGc > 2:853520/1‑133 (MQ=255) tAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAAACGCAGCGTTGc < 1:853520/133‑1 (MQ=255) tAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAAACGCAGCGTTGCCAGAAACGCCATAACt > 1:209859/1‑149 (MQ=255) aaCGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAAACGCAGCGTTGCCAGAAACGCCATAACTcc > 2:488149/1‑149 (MQ=255) aCGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCTGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAACCGCAGCGTTGCCAGAAACGCCATAACTccc < 3:770358/149‑1 (MQ=255) aCGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAAACGCAGCGTTGCCAGAAACGCCATAACTccc < 2:139021/149‑1 (MQ=255) gCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAACCGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTaa < 4:186453/149‑1 (MQ=255) tCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAACCGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAAcc < 3:865648/149‑1 (MQ=255) cACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAAACGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCa > 4:826465/1‑149 (MQ=255) ttCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAAACGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAAc > 2:49370/1‑149 (MQ=255) gcTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAAACGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGaa > 3:911864/1‑149 (MQ=255) gTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAAACGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGAAAGa > 4:100649/1‑149 (MQ=255) gTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAAACGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGAAAGa > 2:425120/1‑149 (MQ=255) tttACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCTCACCAGCACAAAAACGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGaaa > 3:117985/1‑146 (MQ=255) tttACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAAACGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGAAAGAt > 3:917841/1‑149 (MQ=255) tttACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACAGCTGCTGGCTTCCCGCTGATTACCCATCCGCACCAGCACAAAAACGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGaaa < 4:117985/146‑1 (MQ=255) tACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCGACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAAACGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGAAAGATAg < 1:728591/149‑1 (MQ=255) cccGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAACCGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGAAAGATAGTc > 2:244386/1‑149 (MQ=255) cccGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAAACGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGAAAGATAGTc < 1:425120/149‑1 (MQ=255) cGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAACCGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGAAAGATAGTCGa > 4:629564/1‑149 (MQ=255) cGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAAACGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGAAAGATAGTCGa < 3:826465/149‑1 (MQ=255) gCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAACCGCAGCGTTGCAAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAg < 3:753803/149‑1 (MQ=255) tCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAAACGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAGGTc > 3:863159/1‑149 (MQ=255) aCTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAAACGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAGGTCtt > 1:761454/1‑149 (MQ=255) tCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAACCGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGAAAGATAGTCGa > 2:857717/1‑141 (MQ=255) tCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAACCGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGAAAGATAGTCGa < 1:857717/141‑1 (MQ=255) tCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAAACGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCa > 2:373110/1‑126 (MQ=255) tCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAAACGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCa < 1:373110/126‑1 (MQ=255) tCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAAACGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAGGTCTTGc > 3:332650/1‑149 (MQ=255) tCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAAACGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAGGTCTTGc > 2:172905/1‑149 (MQ=255) aTCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAAACGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAGGTCTTGCGt > 1:39721/1‑149 (MQ=255) aTCAGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCTCACCAGCACAAAAACGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAGGTCTTGCGt > 4:789172/1‑149 (MQ=255) tCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAAACGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAGGTCTTGCGta > 1:332710/1‑149 (MQ=255) gTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAACACAAAAACGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAGGTCTTGCGTatat > 2:448292/1‑149 (MQ=255) aaCAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAAACGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCa > 2:682140/1‑111 (MQ=255) aaCAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAAACGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCa < 1:682140/111‑1 (MQ=255) aGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAAACGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAGGTCTTGCGTATATTGCaa > 4:823643/1‑149 (MQ=255) gcgcCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAAACGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAGGTCTTGCGTATATTGCaaa < 4:917841/149‑1 (MQ=255) gcgcCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAAACGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAGGTCTTGCGTATATTGCaaa < 2:475105/149‑1 (MQ=255) cATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAAACGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAGGTCTTGCGTATATTGCAAAGCAc > 2:392578/1‑149 (MQ=255) tCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAACCGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAGGTCTTGCGTATATTGCAAAGCACCa < 1:178359/149‑1 (MQ=255) tCGCCGTAGACCACCCGTTGCTGGCTTGCAGATGATTATCCCTACGCACCAGCACAAAACCGCAGCGTTGCCAGAGACTTCATATCTCCGCGGTGTACACAAAACTCCACGAAGGATAGTCGACGTATTGCGTATACTGCAAAGCACCa < 2:372687/149‑1 (MQ=255) gTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAAACGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAGGTCTTGCGTATATTGCAAAGCACCAGCAAt < 1:732329/149‑1 (MQ=255) ataGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAAACGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAGGTCTTGCGTATATTGCAAAGCACCAGCAATaa < 1:392578/149‑1 (MQ=255) ataGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAAACGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAGGTCTTGCGTATATTGCAAAGCACCAGCAATaa < 4:863159/149‑1 (MQ=255) gCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAAACGCAGCGTTGCCAg > 3:129676/1‑64 (MQ=255) gCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAAACGCAGCGTTGCCAg < 4:129676/64‑1 (MQ=255) cAACCGCTGCTGGCTTCCCGATGATTACCCATCCACACCAGCACAAAAACGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGAAAGATAGTCGATGTCTTGCGTATATTGCAAAGCACCAGCAATAAGTTg > 3:819080/1‑149 (MQ=255) tgctgGCTTCCCGATGATTACCCATCCGCACCAGCACAAAAACGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAGGTCTTGCGTATATTGCAAAGCACCAGCAATAAGTTGCCGCCCt < 2:209859/149‑1 (MQ=255) ctgGCTTCCCGATGATAACCCATCCGCACCAGCACAAAAACGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAGGTCTTGCGTATATTGCAAAGCACCAGCAATAAGTTGCCGCCCtgt < 3:789172/149‑1 (MQ=255) tgGCTTCCCGATGATTACCCATCCGCACCAGCACAAAAACGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAGGTCTTGCGTATATTGCAAAGCACCAGCAATAAGTTGCCGCCCtgtg < 1:353064/149‑1 (MQ=255) ggCTTCCCGATGATTACCCATCCGCACCAGCACAAAAACGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAGGTCTTGCGTATATTGCAAAGCACCAGCAATAAGTTGCCGCCCTGTGc < 2:111886/149‑1 (MQ=255) gCTTCCCGATGATTACCCATCCGCACCAGCACAAAAACGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAGGTCTTGCGTATATTGCAAAGCACCAGCAATAAGTTGCCGCCCTGTGcc > 4:224583/1‑149 (MQ=255) cccGATGATTACCCATCCGCACCAGCACAAAAACGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTGACCGAAAGATAGTCGAGGTCTTGCGTATATTGCAAAGCACCAGCAATAAGTTGCCGCCCTGTGCCTTcc > 4:51286/1‑149 (MQ=255) ccGATGATTACCCATCCGCACCAGCACAAGAACGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAGGTCTTGCGTATATTGCAAAGCACCAGCAATAAGTTGCCGCCCTGTGCCTTccc > 2:169534/1‑149 (MQ=255) cGATGATTACCCATCCGCACCAGCACAACACGGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAGGTCTTGCGTATATTGCAAAGCACCa > 4:567013/1‑120 (MQ=255) cGATGATTACCCATCCGCACCAGCACAAAACCGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAGGTCTTGCGTATATTGCAAAGCACCa > 2:92803/1‑120 (MQ=255) cGATGATTACCCATCCGCACCAGCACAAAACCGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAGGTCTTGCGTATATTGCAAAGCACCa < 3:567013/120‑1 (MQ=255) cGATGATTACCCATCCGCACCAGCACAAAACCGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAGGTCTTGCGTATATTGCAAAGCACCa < 1:92803/120‑1 (MQ=255) cGATGATTACCCATCCGCACCAGCACAAAAACGAAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAACCTCACAGAAAGATAGTCGAGGTCTTGCGTATATTGAAAAGCACCAGCAATAAGTTTACGCCCTATGCCTTCCCg > 4:571265/1‑149 (MQ=255) gatgatTACCCATCCGCACCAGCACAAAACCGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAGGTCTTGCGTATATTGCAAAGCACCAGCAATAAGTTGCCGCCCTGTGCCTTCCCGc > 2:717570/1‑149 (MQ=255) gatgatTACCCATCCGCACCAGCACAAAAACGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAGGTCTTGCGTATATTGCAAAGCACCAGCAATAAGTTGCCGCCCTGTGCCTTCCCGc > 4:845894/1‑149 (MQ=255) gatTACCCATCCGCACCAGCACAAAAACGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAGGTCTTGCGTATATTGCAAAGCACCAGCAATAAGTTGCCGCCCTGTGCCTTCCCGCTGa < 2:332710/149‑1 (MQ=255) atTACCCATCCGCACCAGCACAAAAACGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAGGTCTTGCGTATATTGCAAAGCACCAGCAATAAGTTGCCGCCCTGTGCCTTCCCGCTGAc < 3:823643/149‑1 (MQ=255) aCCCATCCGCACCAGCACAAACCCTCAGCGTCGCCAAAAACGCCATAACTCCCCGGTGTAACCAACACTCACCGAAACATACTCGAGCTCTTGCGTAGATTGCAAACCACCAGCAATAAGTTGCCGCCCTGTGCCTTCCCGCTGACGAg < 2:769431/149‑1 (MQ=255) ccATCCGCACCAGCACAAAACCGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAGGTCTTGCGTATATTGCAAAGCACCAGCAATAAGTTGCCGCCCTGTGCCTTCCCGCTGACGAGcc > 1:420370/1‑149 (MQ=255) ccATCCGCACCAGCACAAAAACGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAGGTCTTGCGTATATTGCAAAGCACCAGCAATAAGTTGCCGCCCTGTGCCTTCCCGCTGACGAGcc > 2:855039/1‑149 (MQ=255) ccATCCGCACCAGCACAAAAACGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAGGTCTTGCGTATATTGCAAAGCACCAGCAATAAGTTGCCGCCCTGTGCCTTCCCGCTGACGAGcc > 4:860164/1‑149 (MQ=255) tCCGCACCAGCACAAAAACGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAGGTCTTGCGTATATTGCAAAGCACCAGCAATAAGTTGCCGCCCTGTGCCTTCCCGCTGACGAGCCGGa < 3:845894/149‑1 (MQ=255) tCCGCACCAGCACAAAAACGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAGATCTTGCGTATATTGCAAAGCACCAGCAATAAGTTGCCGCCCTGTGCCTTCCCGCTGAc > 2:712610/1‑141 (MQ=255) tCCGCACCAGCACAAAAACGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAGATCTTGCGTATATTGCAAAGCACCAGCAATAAGTTGCCGCCCTGTGCCTTCCCGCTGAc < 1:712610/141‑1 (MQ=255) ccGCACCAGCACAAAAACGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAGGTCTTGCGTATATTGCAAAGCACCAGCAATAAGTTGCCGCCCTGTGCCTTCCCGCTGACGAGCCGGAt < 1:507218/149‑1 (MQ=255) cGCACCAGCACAAAAACGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAGGTCTTGCGTATATTGCAAAGCACCAGCAATAAGTTGCCGCCCTGTGCCTTCCCGCTGACGAGCCGGATg < 3:92671/149‑1 (MQ=255) cACCAGCACAAAAACGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAGGTCTTGCGTATATTGCAAAGCACCAGCAATAAg > 1:676856/1‑111 (MQ=255) cACCAGCACAAAAACGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAGGTCTTGCGTATATTGCAAAGCACCAGCAATAAg < 2:676856/111‑1 (MQ=255) cACCAGCACAAAAACGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAGGTCTTGCGTATATTGCAAAGCACCAGCAATAAGTTGCCGCCCTGTGCCTTCCCGCTGACGAGCCGGATGaa < 2:910876/149‑1 (MQ=255) aCCAGCACAAAAACGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAGGTCTTGCGTATATTGCAAAGCACCAGCAATAAGTTGCCGCCCTGTGCCTTCCCGCTGACGAGCCGGATGAAc < 4:819080/149‑1 (MQ=255) aCCAGCACAAAAACGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAGGTCTTGCGTATATTGCAAAGCACCAGCAATAAGTTGCCGCCCTGTGCCTTCCCGCTGACGAGCCGGATGAAc < 3:22663/149‑1 (MQ=255) aCCAGCACAAAAACGCAGCGTTGCCAGAAACGCAATAACTCCCCGGTGTAACAAAAACTCACCGAAAGATAGTCGAGGTCTTGCGTATATTGCAAAGCACCAGCAATAAGTTGCCGCCCTGTGCCTTCCCGCTGACGAGCCGGATGAAc < 1:627886/149‑1 (MQ=255) aGCACAAAAACGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAGGTCTTGCGTATATTGCAAAGCACCAGCAATAAGTTGCCGCCCTGTGCCTTCCCGCTGACGAGCCGGATGAACTGc < 3:740001/149‑1 (MQ=255) acaAAACCGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAGGTCTTGCGTATATTGCAAAGCACCAGCAATAAGTTGCCGCCCTGTGCCTTCCCGCTGACGAGCCGGATGAACTGCTAt > 2:443191/1‑149 (MQ=255) caAAAACGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAGGTCTTGCGTATATTGCAAAGCACCAGCAATAAGTTGCCGCCCTGTGCCTTCCCGCTGACGAGCCGGATGAACTGCTATc > 2:536909/1‑149 (MQ=255) aaaaACGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAGGTCTTGCGTATATTGCAAAGCACCAGCAATaa > 1:922466/1‑101 (MQ=255) aaaaACGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAGGTCTTGCGTATATTGCAAAGCACCAGCAATaa < 2:922466/101‑1 (MQ=255) | GCTTGCGCATCGCGACGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAACCGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAGGTCTTGCGTATATTGCAAAGCACCAGCAATAAGTTGCCGCCCTGTGCCTTCCCGCTGACGAGCCGGATGAACTGCTATC > NC_000913/2594199‑2594480 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |