Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,684,207 C→T 6.4% intergenic (+151/+47) yphH → / ← glyA putative DNA‑binding transcriptional regulator/serine hydroxymethyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,684,2070CT 6.4% 348.3 / 10.1 110intergenic (+151/+47)yphH/glyAputative DNA‑binding transcriptional regulator/serine hydroxymethyltransferase
Reads supporting (aligned to +/- strand):  ref base C (50/52);  new base T (3/4);  total (54/56)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.48e-01

Only 100 of 112 total aligned reads displayed.

TAACGTAGAAAGGCTTCCCGAAGGAAGCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGCACATTGACAGCAAATCACCGTTTCGCTTATGCGTAAACCGGGTAACGTGCGCAGATGTCGAGAACTTTACCTTTGATGCGCTCGATAACGGCTTCATCATTGATGCTGTCCAGCACGTCACACATCCA  >  NC_000913/2684065‑2684355
                                                                                                                                              |                                                                                                                                                    
 aaCGTAGAAAGGCTTCCCGAAGGAAGCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATGCGGCGTGAACGCCTTATCCGGc                                                                                                                                               <  2:572058/149‑1 (MQ=37)
  aCGTAGAAAGGCTTCCCGAAGGAAGCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACGCCTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCa                                                                                                                                              >  3:548750/1‑149 (MQ=255)
     tAGAAAGGCTTCCCGAAGGAAGCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATGCGGCGTGAACGCCTTATCCgg                                                                                                                                                <  4:847985/144‑1 (MQ=37)
      aGAAAGGCTTCCCGAAGGAAGCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGaa                                                                                                                                          >  2:600907/1‑149 (MQ=255)
        aaaGGCTTCCCGAAGGAAGCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGaaca                                                                                                                                        >  2:733058/1‑149 (MQ=255)
         aaGGCTTCCCGAAGGAAGCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGaacaa                                                                                                                                       <  2:409981/149‑1 (MQ=255)
            gCTTCCCGAAGGAAGCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGa                                                                                                                                    <  4:543911/149‑1 (MQ=255)
             cTTCCCGAAGGAAGCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAg                                                                                                                                   >  1:806905/1‑149 (MQ=255)
              ttCCCGAAGGAAGCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGc                                                                                                                                  >  3:767294/1‑149 (MQ=255)
              ttCCCGAAGGAAGCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGc                                                                                                                                  >  1:929131/1‑149 (MQ=255)
                cccGAAGGAAGCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGcac                                                                                                                                <  3:230519/149‑1 (MQ=255)
                 ccGAAGGAAGCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGcaca                                                                                                                               >  3:139165/1‑149 (MQ=255)
                 ccGAAGGAAGCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGcaca                                                                                                                               >  1:208274/1‑149 (MQ=255)
                  cGAAGGAAGCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATGCGGCGTGAACGCCTTATCCGGc                                                                                                                                               <  3:346593/132‑1 (MQ=25)
                  cGAAGGAAGCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATGCGGCGTGAACGCCTTATCCGGc                                                                                                                                               >  4:346593/1‑132 (MQ=21)
                   gaaggaagCCTTGATGATCATAAACGAAAAATTGCCTGATTCGCTACGCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCTGGATAAGGCGGCCACCCCTCTTCCCGCATGCACCGCGATcacaat                                                                                                                             <  2:399954/149‑3 (MQ=21)
                           ccTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGGGGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGCACATTGACAGCaa                                                                                                                     >  3:507295/1‑149 (MQ=255)
                            cTTGATGATCATAAACGAAAAATTGACTGATGCGCTACGATTATCATGCCTACACGGAGATTGCAGTATATTGAATTTGCAAAGTTTTGTCGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGCACATTGACAGCaga                                                                                                                    >  4:228076/1‑147 (MQ=21)
                              tgatgaTCATAAACGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGCACATTGAc                                                                                                                          <  3:94003/141‑1 (MQ=255)
                                atgatCATAAACGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAGATTGCGATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGCACATTGACa                                                                                                                         <  2:906916/140‑1 (MQ=255)
                                atgatCATAAACGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAGATTGCGATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGCACATTGACa                                                                                                                         >  1:906916/1‑140 (MQ=255)
                                    tCATAAACGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGCACATTGACa                                                                                                                         >  3:372457/1‑136 (MQ=255)
                                    tCATAAACGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGCACATTGACa                                                                                                                         <  4:372457/136‑1 (MQ=255)
                                    tCATAAACGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGCACATTGACAGCAAAt                                                                                                                   <  4:697633/142‑1 (MQ=255)
                                    tCATAAACGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGCACATTGACAGCAAAt                                                                                                                   >  3:697633/1‑142 (MQ=255)
                                      aTAAACGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGCACATTGACAGCAAATCACCGTTTc                                                                                                          >  3:192342/1‑149 (MQ=255)
                                      aTAAACGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGCACATTGACAGCAAATCACCGTTTc                                                                                                          <  3:390389/149‑1 (MQ=255)
                                           cGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGCACATTGACAGCAAATCACCGTTTCGCTTa                                                                                                     <  4:46212/149‑1 (MQ=255)
                                            gAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGCACATTGACAGCAAATCACCGTTTCGCTTAt                                                                                                    >  1:274968/1‑149 (MQ=255)
                                             aaaaaTTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGCACATTGACAGCAAATCACCGTTTCGCTTATg                                                                                                   <  3:792576/149‑1 (MQ=255)
                                              aaagTTGCCTGATGCGCTCAGCTTATCGGTCCTAGACTGGGAAAGCAATATTTTGAATTTCGCTTGTTATGTCGGCCGGGTGAGGTGATGACGGTGCATCTGCAATGAACAATGAGCACATTGACAGCAAATCACCGTATCGCTCATgt                                                                                                  >  2:496243/1‑148 (MQ=255)
                                              aaaaTTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGCACATTGACAGCAAATCACCGTTTCGCTTATGc                                                                                                  <  1:600907/149‑1 (MQ=255)
                                                 aTTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGCACATTGACAGCAAATCACCGTTTCGCTTATGCGTa                                                                                               >  4:440589/1‑149 (MQ=255)
                                                  ttGCCTGATGCGCTACGCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGCACATTGACAGCAAATCACCGTTTCGCTTATGCGTaa                                                                                              >  3:767405/1‑149 (MQ=255)
                                                     ccTGATGCGCTACGCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGCACATTGACAGCAAATCACCGTTTCGCTTATGCGTAAAcc                                                                                           >  3:499784/1‑149 (MQ=255)
                                                       tGATGCGCTACGCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGCACATTGACAGCAAATCACCGTTTCGCTTATGCGTAAACCgg                                                                                         <  3:440589/149‑1 (MQ=255)
                                                        gATGCGCTACGCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGCACATTGACAGCAAATCACCGTTTCGCTTATGCGTAAACCggg                                                                                        >  4:622439/1‑149 (MQ=255)
                                                             gcTACGCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGCACATTGACAGCAAATCACCGTTTCGCTTATGCGTAAACCGGGTAACg                                                                                   >  1:691085/1‑149 (MQ=255)
                                                               tACGCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGCACATTGACAGCAAATCACCGTTTCGCTTATGCGTAAACCGGGTAACGTg                                                                                 >  3:380370/1‑149 (MQ=255)
                                                                  gCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGCACATTGACAGCAAATCACCGTTTCGCTTATGCGTAAACCGGGTAACGTgcgc                                                                              >  4:486505/1‑149 (MQ=255)
                                                                    ttATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGCACATTGACAGCAAATCACCGTTTCGCTTATGCGTAAACCgg                                                                                         <  2:516673/136‑1 (MQ=255)
                                                                    ttATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGCACATTGACAGCAAATCACCGTTTCGCTTATGCGTAAACCgg                                                                                         >  1:516673/1‑136 (MQ=255)
                                                                     tATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGCACATTGACAGCAAATCACCGTTTCGCTTATGCGTAAACCGGGTAACGTGCGCAGa                                                                           <  3:422536/149‑1 (MQ=255)
                                                                      aTCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGCACATTGACAGCAAATCACCGTTTCGCTTATGCGTAAACCGGGTAACGTGCGCAGAt                                                                          <  3:341127/149‑1 (MQ=255)
                                                                      aTCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGCACATTGACAGCAAATCACCGTTTCGCTTATGCGTAAACCGGGTAACGTGCGCAGAt                                                                          <  3:802770/149‑1 (MQ=255)
                                                                       tCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGCACATTGACAGCAAATCACCGTTTCGCTTATGCGTAAACCGGGTAACGTGCGCAGATg                                                                         >  2:264481/1‑149 (MQ=255)
                                                                          ggCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGCACATTGACAGCAAATCACCGTTTCGCTTATGCGTAAACCGGGTAACGTGCGCAGATGTCg                                                                      <  4:209331/149‑1 (MQ=255)
                                                                           gCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGCACATTGACAGCAAATCACCGTTTCGCTTATGCGTAAACCGGGTAACGTGCGCAGATGTCga                                                                     <  2:638784/149‑1 (MQ=255)
                                                                           gCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGCACATTGACAGCAAATCACCGTTTCGCTTATGCGTAAACCGGGTAACGTGCGCAGATGTCga                                                                     <  4:579234/149‑1 (MQ=255)
                                                                               acacGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGCACATTGACAGCAAATCACCGTTTCGCTTATGCGTAAACCGGGTAACGTGCGCAGATGTCGAGAAc                                                                 >  3:52176/1‑149 (MQ=255)
                                                                               acacGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGCACATTGACAGCAAATCACCGTTTCGCTTATGCGTAAACCGGGTAACGTGCGCAGATGTCGAGAAc                                                                 >  2:307885/1‑149 (MQ=255)
                                                                               acacGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGCACATTGACAGCAAATCACCGTTTCGCTTATGCGTAAACCGGGTAACGTGCGCAGATGTCGAGAAc                                                                 <  1:374119/149‑1 (MQ=255)
                                                                                cacGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGCACATTGACAGCAAATCACCGTTTCGCTTATGCGTAAACCGGGTAACGTGCGCAGATGTCGAGAACt                                                                <  2:691085/149‑1 (MQ=255)
                                                                                 acGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGCACATTGACAGCAAATCACCGTTTCGCTTATGCGTAAACCGGGTAACGTGCGCAGATGTCGAGAACtt                                                               >  1:819295/1‑149 (MQ=255)
                                                                                 acGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGAGAAGGCGTTCACGCCGCATCCGGCCAGAACAACGAGCACATTGACAGCAAATCACCGTTTCGCTTATGCGTAAACCGGGTAACGTGCGCAGATGTCGAGAACtt                                                               >  2:96943/1‑149 (MQ=255)
                                                                                   ggAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGCACATTGACAGCAAATCACCGTTTCGCTTAt                                                                                                    >  2:202525/1‑110 (MQ=255)
                                                                                   ggAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGCACATTGACAGCAAATCACCGTTTCGCTTAt                                                                                                    <  1:202525/110‑1 (MQ=255)
                                                                                     agaTTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGCACATTGACAGCAAATCACCGTTTCGCTTATGCGTAAACCGGGTAACGTGCGCAGATGTCGAGAACTTTAcc                                                           >  1:303351/1‑149 (MQ=255)
                                                                                          gCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGCACATTGACAGCAAATCACCGTTTCGCTTATGCGTAAACCGGGTAACGTGCGCAGATGTCGAGAACTTTACCTTTGa                                                      <  4:870404/149‑1 (MQ=255)
                                                                                          gCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGCACATTGACAGCAAATCACCGTTTCGCTTATGCGTAAACCGGGTAACGTGCGCAGATGTCGAGAACTTTACCTTTGa                                                      <  4:315609/149‑1 (MQ=255)
                                                                                          gCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGCACATTGACAGCAAATCACCGTTTCGCTTATGCGTAAACCGGGTAACGTGCGCAGATGTCGAGAACTTTACCTTTGa                                                      <  3:486505/149‑1 (MQ=255)
                                                                                          gCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGCACATTGACAGCAAATCACCGTTTCGCTTATGCGTAAACCGGGTAACGTGCGCAGATGTCGAGAACTTTACCTTTGa                                                      <  1:744480/149‑1 (MQ=255)
                                                                                           cAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGCACATTGACAGCAAATCACCGTTTCGCTTATGCGTAAACCGGGTAACGTGCGCAGATGTCGAGAACTTTACCTTTGAt                                                     <  2:326351/149‑1 (MQ=255)
                                                                                           cAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGCACATTGACAGCAAATCACCGTTTCGCTTATGCGTAAACCGGGTAACGTGCGCAGATGTCGAGAACTTTACCTTTGAt                                                     <  4:72784/149‑1 (MQ=255)
                                                                                            aaTATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGCACATTGACAGCAAATCACCGTTTCGCTTATGCGTAAACCGGGTAAc                                                                                    <  2:466122/117‑1 (MQ=255)
                                                                                            aaTATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGCACATTGACAGCAAATCACCGTTTCGCTTATGCGTAAACCGGGTAAc                                                                                    >  1:466122/1‑117 (MQ=255)
                                                                                            aaTATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGCACATTGACAGCAAATCACCGTTTCGCTTATGCGTAAACCGGGTAACGTGCGCAGATGTCGAGAACTTTACCTTTGATg                                                    >  2:283729/1‑149 (MQ=255)
                                                                                               atatTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGCACATTGACAGCAAATCACCGTTTCGCTTATGCGTAAACCGGGTAACGTGCGCAGATGTCGAGAACTTTACCTTTGATgcgc                                                 <  3:397652/149‑1 (MQ=255)
                                                                                                  ttGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGCACATTGACAGCAAATCACCGTTTCGCTTATGCGTAAACCGGGTAACGTGCGCAGATGTCGAGAACTTTACCTTTGATGCGCTCg                                              <  2:720538/149‑1 (MQ=255)
                                                                                                   tGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGCACATTGACAGCAAATCACCGTTTCGCTTATGCGTAAACCGGGTAACGTGCGCAGATGTCGAGAACTTTACCTTTGATGCGCTCGa                                             <  1:663014/149‑1 (MQ=255)
                                                                                                    gAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGCACATTGACAGCAAATCACCGTTTCGCTTATGCGTAAACCgg                                                                                         >  3:1068/1‑104 (MQ=255)
                                                                                                    gAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGCACATTGACAGCAAATCACCGTTTCGCTTATGCGTAAACCgg                                                                                         <  4:1068/104‑1 (MQ=255)
                                                                                                      aTTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGCACATTGACAGCAAATCACCGTTTCGCTTATGCGTAAACCGGGTAACGTGCGCAGATGTCGAGAACTTTACCTTTGATGCGCTCGATaa                                          <  2:929131/149‑1 (MQ=255)
                                                                                                      aTTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGCACATTGACAGCAAATCACCGTTTCGCTTATGCGTAAACCGGGTAACGTGCGCAGATGTCGAGAACTTTACCTTTGATGCGCTCGATaa                                          >  4:859719/1‑149 (MQ=255)
                                                                                                           cAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGCACATTGACAGCAAATCACCGTTTCGCTTATGCGTAAACCGGGTAACGTGCGCAGATGTCGAGAACTTTACCTTTGATGCGCTCGATAACGGCt                                     >  1:507443/1‑149 (MQ=255)
                                                                                                           cAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGCACATTGACAGCAAATCACCGTTTCGCTTATGCGTAAACCGGGTAACGTGCGCAGATGTCGAGAACTTTACCTTTGATGCGCTCGATAACGGCt                                     <  4:767405/149‑1 (MQ=255)
                                                                                                            aaaGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGCACATTGACAGCAAATCACCGTTTCGCTTATGCGTAAACCGGGTAACGTGCGCAGATGTCGAGAACTTTACCTTTGATGCGCTCGATAACGGCtt                                    >  3:672760/1‑149 (MQ=255)
                                                                                                            aaaGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGCACATTGACAGCAAATCACCGTTTCGCTTATGCGTAAACCGGGTAACGTGCGCAGATGTCGAGAACTTTACCTTTGATGCGCTCGATAACGGCtt                                    >  1:609654/1‑149 (MQ=255)
                                                                                                            aaaGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGCACATTGACAGCAAATCACCGTTTCGCTTATGCGTAAACCGGGTAACGTGCGCAGATGTCGAGAACTTTACCTTTGATGCGCTCGATAACGGCtt                                    >  2:213805/1‑149 (MQ=255)
                                                                                                             aaGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGCACATTGACAGCAAATCACCGTTTCGCTTATGCGTAAACCGGGTAACGTGCGCAGATGTCGAGAACTTTACCTTTGATGCGCTCGATAACGGCTtc                                   >  4:235469/1‑149 (MQ=255)
                                                                                                                ttttGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAGGAACACCGAGCACATTGACAGCAAATCACCGTGTCGCTTATGCGTAAACCGGGTAACGTGCGCAGATGTCGAGAACTTTACCTTTGATGCGCTCGATAACGGCTtcatc                                <  3:646221/149‑1 (MQ=255)
                                                                                                                ttttGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGCACATTGACAGCAAATCACCGTTTCGCTTATGCGTAAACCGGGTAACGTGCGCAGATGTCGAGAACTTTACCTTTGATGCGCTCGATAACGGCTtcatc                                >  1:390366/1‑149 (MQ=255)
                                                                                                                 tttGTAGGCCGGATAAGGCGGTCACGCCGCATGCGGCATGAACAACGAGCACATTGACAGCAAATCACCGTTTCGCGTATGTGTAAACCGGGTAACGTGCGCAGATGTCGAGAACTTTACCTTTGATGCGCTGGATATCGGCTtcatca                               <  4:650700/149‑1 (MQ=38)
                                                                                                                  ttGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGCACATTGACAGCAAATCACCGTTTCGCTTATGCGTAAACCGGGTAACGTGCGCAGATGTCGAGAACTTTACCTTTGATGCGCTCGATAACGGCTTcatcat                              <  1:307885/149‑1 (MQ=255)
                                                                                                                     catgCCGGATGCGGCGTGAACGCCTTATCCGGCATGAACAACGAGCACATTGACAGCAAATCACCGTTTCGCTTATGCGTAAACCGGGTAACGTGCGCAGATGTCGAGAACTTTACCTTTGATGCGCTCGATAACGGCTTCATCATTGa                           >  1:351091/4‑149 (MQ=38)
                                                                                                                      atgCCGGATGCGGCGTGAACGCCTTATCCGGCATGAACAACGAGCACATTGACAGCAAATCACCGTTTCGCTTATGCGTAAACCGGGTAACGTGCGCAGATGTCGAGAACTTTACCTTTGATGCGCTCGATAACGGCTTCATCATTGAt                          >  2:146038/3‑149 (MQ=38)
                                                                                                                        gCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGCACATTGACAGCAAATCACCGTTTCGCTTATGCGTAAACCGAGTAAGGTGCGCAGATGTCGAGAACTTTACCTTTGATGCGCTCGATAACGGCTTCATCATTGATga                        >  2:670260/1‑148 (MQ=255)
                                                                                                                         ccGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGCACATTGACAGCAAATCACCGTTTCGCTTATGCGTAAACCGGGTAACGTGCGCAGATGTCGAGAACTTTACCTTTGATGCGCTCGATAACGGCTTCATCATTGATGCt                       <  4:204664/149‑1 (MQ=255)
                                                                                                                              tAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGCACATTGACAGCAAATCACGGTTTCGCTTATGCGTAAACCGGGTAACGTGCGCAGATGTCGAGAACATTACCTTTGATGCGCCCGATAACGGCTTCATCATTGATGCTGTCCa                  <  3:228076/149‑1 (MQ=255)
                                                                                                                              tAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGCACATTGACAGCAAATCACCGTTTCGCTTATGCGTAAACCGGGTAACGTGCGCAGATGTCGAGAACTTTACCTTTGATGCGCTCGATAACGGCTTCATCATTGATGCTGTCCa                  <  3:276773/149‑1 (MQ=255)
                                                                                                                               aaGGCGTTCACGCCGCATCCGGCATGAACAACGAGCACATTGACAGCAAATCACCGTTTCGCTTATGCGTAAACCGGGTAACGTGCGCAGATGTCGAGAACTTTACCTTTGATGCGCTCGATAACGGCTTCATCATTGATGCTGTCCAg                 <  3:433181/149‑1 (MQ=255)
                                                                                                                                  gCGTTCACGCCGCATCCGGCATGAACAACGAGCACATTGACAGCAAATCACCGTTTCGCTTATGCGTAAACCGGGTAACGTGCGCAGATGTCGAGAACTTTACCTTTGATGCGCTCGATAACGGCTTCATCATTGATGCTGTCCAGCAc              >  4:5919/1‑149 (MQ=255)
                                                                                                                                    gTTCATGCCGGATGCGGCGTGAACAACGAGCACATTGACAGCAAATCACCGTTTCGCTTATGCGTAAACCGGGTAACGTGCGCAGATGTCGAGAACTTTACCTTTGATGCGCTCGATAACGGCTTCATCATTGATGCTGTCCAGCACGt            >  2:174816/1‑149 (MQ=255)
                                                                                                                                    gTTCACGCCGCATCCGGCATGAACAACGAGCACATTGACAGCAAATCACCGTTTCGCTTATGCGTAAACCGGGTAACGTGCGCAGATGTCGAGAACTTTACCTTTGATGCGCTCGATAACGGCTTCATCATTGATGCTGTCCAGCACGt            <  4:767294/149‑1 (MQ=255)
                                                                                                                                     ttCACGCCGCATCCGGCATGAACAACGAGCACATTGACAGCAAATCACCGTTTCGCTTATGCGTAAACCggg                                                                                        <  2:680596/72‑1 (MQ=255)
                                                                                                                                     ttCACGCCGCATCCGGCATGAACAACGAGCACATTGACAGCAAATCACCGTTTCGCTTATGCGTAAACCggg                                                                                        >  1:680596/1‑72 (MQ=255)
                                                                                                                                     ttCACGCCGCATCCGGCATGAACAACGAGCACATTGACAGCAAATCACCGTTTCGCTTATGCGTAAACCGGGTAACGTGCGCAGATGTCGAGAACTTTACCTTTGATGCGCTCGATAACGGCTTCATCATTGATGCTGTCCAGCACGTc           >  4:563957/1‑149 (MQ=255)
                                                                                                                                      gaaCGCCTTATCCGGCATGAACAACGAGCACATTGACAGCAAATCACCGTTTCGCTTATGCGTAAACCGGGTAACGTGCGCAGATGTCGAGAACTTTACCTTTGATGCGCTCGATAACGGCTTCATCATTGATGCTGTCCAGCACGTca          <  1:146038/147‑1 (MQ=255)
                                                                                                                                         tgccggATGCGGCGTGAACAACGAGCACATTGACAGCAAATCACCGTTTCGCTTATGCGTAAACCGGGTAACGTGCGCAGATGTCGAGAACTTTACCTTTGATGCGCTCGATAACGGCTTCATCATTGATGCTGTCCAGCACGTcacac       >  3:677599/2‑149 (MQ=255)
                                                                                                                                              cATCCGGCATGAACAACGAGCACATTGACAGCAAATCACCGTTTCGCTTATGCGTAAACCGGGTAACGTGCGCAGATGTCGAGAACTTTACCTTTGATGCGCTCGATAACGGCTTCATCATTGATGCTGTCCAGCACGTCACACATcca  <  2:303351/149‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                                    
TAACGTAGAAAGGCTTCCCGAAGGAAGCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGCACATTGACAGCAAATCACCGTTTCGCTTATGCGTAAACCGGGTAACGTGCGCAGATGTCGAGAACTTTACCTTTGATGCGCTCGATAACGGCTTCATCATTGATGCTGTCCAGCACGTCACACATCCA  >  NC_000913/2684065‑2684355

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: