| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,706,607 | A→C | 8.9% | L173R (CTC→CGC) ‡ | lepA ← | back‑translocating elongation factor EF4, GTPase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,706,607 | 0 | A | C | 8.9% | 283.7 / 20.7 | 90 | L173R (CTC→CGC) ‡ | lepA | back‑translocating elongation factor EF4, GTPase |
| Reads supporting (aligned to +/- strand): ref base A (41/41); new base C (4/4); total (45/45) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.54e-01 | |||||||||||
GTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTCAATTTCTTCCGCCACGCGTTCAGGATCGGCTGCCGGCAGGTCAATCTTGTTCAGTACCGGCACAACTTCGA > NC_000913/2706462‑2706754 | gTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGaa < 2:641552/149‑1 (MQ=255) ccTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTcc < 1:589188/149‑1 (MQ=255) cTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCTCGAACGTctg < 4:559365/149‑3 (MQ=255) ttACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCTCGAACGTctgg < 3:494897/149‑4 (MQ=255) gCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGcaca > 2:489897/1‑149 (MQ=255) gCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGc < 2:671167/149‑1 (MQ=255) tttttAATACGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGt > 3:335962/1‑149 (MQ=255) tttAATACGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGttt < 2:896543/149‑1 (MQ=255) tAATACGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTc < 4:219198/149‑1 (MQ=255) aTACGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGc < 3:789887/149‑1 (MQ=255) aTACGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGc > 4:79490/1‑149 (MQ=255) cGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGa < 3:318655/149‑1 (MQ=255) gTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGcc > 1:769040/1‑130 (MQ=255) gTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGcc < 2:769040/130‑1 (MQ=255) tGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGaa < 4:39370/110‑1 (MQ=255) tGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGaa > 3:39370/1‑110 (MQ=255) tGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCa > 3:886541/1‑149 (MQ=255) tGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCa > 1:32185/1‑149 (MQ=255) tGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCa > 4:448306/1‑149 (MQ=255) aaaCAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCTGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCAcc > 2:255954/1‑149 (MQ=255) aaaCAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCAcc > 4:884436/1‑149 (MQ=255) acGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCTCGAACGTctggt > 3:419389/1‑107 (MQ=255) acGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCTCGAACGTctggt < 4:419389/111‑5 (MQ=255) acGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTc < 4:886541/149‑1 (MQ=255) gCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCgg > 4:392195/1‑149 (MQ=255) ccAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTg > 2:809123/1‑149 (MQ=255) cAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCAGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTgg < 4:609998/149‑1 (MQ=255) gTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGa > 2:349792/1‑149 (MQ=255) gTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGa < 1:827696/149‑1 (MQ=255) ttGTCGAACCATGAGTCGATTATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGAt > 2:654543/1‑149 (MQ=255) ttGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGAt < 4:422239/149‑1 (MQ=255) ttGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGAt > 3:282108/1‑149 (MQ=255) aaCCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCcgac > 4:259025/1‑149 (MQ=255) ccATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCcgacga < 3:392195/149‑1 (MQ=255) aTGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGata > 4:56578/1‑149 (MQ=255) aTGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGata < 2:32185/149‑1 (MQ=255) tGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGatat < 1:17103/149‑1 (MQ=255) gAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATc < 2:505824/149‑1 (MQ=255) aGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCt < 2:600969/149‑1 (MQ=255) tCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCTCGAACGTc < 4:270140/80‑1 (MQ=255) tCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCTCGAACGTc > 3:270140/1‑80 (MQ=255) gATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGc < 1:377182/134‑1 (MQ=255) gATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGc > 2:377182/1‑134 (MQ=255) gATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTCaa < 1:489897/149‑1 (MQ=255) gATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTCaa > 2:2952/1‑149 (MQ=255) aTAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTCAAt < 3:48548/149‑1 (MQ=255) ttAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTCAATttct < 2:318953/149‑1 (MQ=255) aGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTCAATttcttc < 1:474591/149‑1 (MQ=255) ccTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTCAATTTCTTCCGcc > 2:57453/1‑149 (MQ=255) cTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTCAATTTCTTCCGCCa > 3:750766/1‑149 (MQ=255) cTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTCAATTTCTTCCGCCa > 1:757376/1‑149 (MQ=255) aCGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTCAATTTCTTCCGCCACGCGt > 2:468202/1‑149 (MQ=255) aCGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTCAATTTCTTCCGCCACGCGt > 3:293544/1‑149 (MQ=255) gggCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTCAATTTCTTCCGCCACGCGTTc < 1:468202/149‑1 (MQ=255) ggCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTc < 3:504276/58‑1 (MQ=255) ggCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTc > 4:504276/1‑58 (MQ=255) ccTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTCAATTTCTTCCGCCACGCGTTCAgg < 2:757376/149‑1 (MQ=255) cTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTCAATTTCTTCCGCCACGCGTTCAGGa > 3:813987/1‑149 (MQ=255) ttCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTc < 2:15514/96‑1 (MQ=255) ttCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTc > 1:15514/1‑96 (MQ=255) ttCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTCAATTTCTTCCGCCACGCGTTCAGGAt > 4:804985/1‑149 (MQ=255) tCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTCAATTTCTTCCGCCACGCGTTCAGGATc < 4:263373/149‑1 (MQ=255) aTCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTCAATTTCTTCCGCCACGCGTTCAGGATCGGCTg > 4:798128/1‑149 (MQ=255) cGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTCAATTTCTTCCGCCACGCGTTCAGGATCGGCTGcc < 1:809123/149‑1 (MQ=255) cTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTCAATTTCTTCCGCCACGCGTTCAGGATCGGCTGCCGGc > 4:286457/1‑149 (MQ=255) cTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTCAATTTCTTCCGCCACGCGTTCAGGATCGGCTGCCGGc < 1:874681/149‑1 (MQ=255) ttCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTCAATTTCTTCCGCCACGCGTTCAGGATCGGCTGCCGGCa > 4:495938/1‑149 (MQ=255) ttCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTCAATTTCTTCCGCCACGCGTTCAGGATCGGCTGCCGGCa > 2:214831/1‑149 (MQ=255) tCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTCAATTTCTTCCGCCACGCGTTCAGGATCGGCTGCCGGCAg < 3:266051/149‑1 (MQ=255) cggcggcggAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTCAATTTCTTCCGCCACGCGTTCAGGATCGGCTGCCGGCAGGt < 1:57453/149‑1 (MQ=255) ggcggcggAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTCAATTTCTTCCGCCACGCGTTCAGGATCGGCTGCCGGCAGGTc > 2:463184/1‑149 (MQ=255) ggAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTCAATTTCTTCCGCCACGCGtt < 1:415684/121‑1 (MQ=255) ggAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTCAATTTCTTCCGCCACGCGtt > 2:415684/1‑121 (MQ=255) aaTGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTCAATTTCTTCCGCCACGCGTTCAGGATCGGCTGCCGGCAGGTCAATCTTGt < 2:700864/149‑1 (MQ=255) aTGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTCAATTTCTTCCGCCACGCGTTCAGGATCGGCTGCCGGCAGGTCAATCTTGtt < 1:100913/149‑1 (MQ=255) aTGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTCAATTTCTTCCGCCACGCGTTCAGGATCGGCTGCCGGCAGGTCAATCTTGtt < 4:783336/149‑1 (MQ=255) gcgcACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTTGATGCCGACGATATCTTCAATTTCTTCCGCCACGCGTTCAGGATCGGCTGCCGGCAGGTCAATCTTGTTCAGTa > 2:600173/1‑149 (MQ=255) gcACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTCAATTTCTTCCGCCACGCGTTCAGGATCGGCTGCCGGCAGGTCAATCTTGTTCAGTAcc > 2:657988/1‑149 (MQ=255) aCCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTCAATTTCTTCCGCCACGCGTTCAGGATCGGCTGCCGGCAGGTCAATCTTGtt > 1:611755/1‑140 (MQ=255) aCCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTCAATTTCTTCCGCCACGCGTTCAGGATCGGCTGCCGGCAGGTCAATCTTGtt < 2:611755/140‑1 (MQ=255) cAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTCAATTTCTTCCGCCACGCGTTCAGGATCGGCTGCCGGCAGGTCAATCTTGTTCAGTACCGGca < 1:121827/149‑1 (MQ=255) aggACGTTCTCGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTCAATTTCTTCCGCCACGCGTTCAGGATCGGCTGCCGGCAGGTCAATCTTGTTCAGTACCGGca > 4:260948/3‑149 (MQ=255) ggACGTTCTCGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTCAATTTCTTCCGCCACGCGTTCAGGATCGGCTGCCGGCAGGTCAATCTTGTTCAGTACCGGcac > 4:12790/2‑149 (MQ=255) aGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTCAATTTCTTCCGCCACGCGTTCAGGATCGGCTGCCGGCAGGTCAATCTTGTTCAGTACCGGcac < 2:109609/149‑1 (MQ=255) aGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGAGGCGTCGATGCCGACGATATCTTCAATTTCTTCCGCCACGCGTTCAGGATCGGCTGCCGGCAGGTCAATCTTGTTCAGTACCGGcac > 3:613096/1‑149 (MQ=255) gACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTCAATTTCTTCCGCCACGCGTTCAGGATCGGCTGCCGGCAGGTCAATCTTGTTCAGTACCGGcaca > 3:110623/1‑149 (MQ=255) gTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTCAATTTCTTCCGCCACGCGTTCAGGATCGGCTGCCGGCAGGTCAATCTTGTTTAGTACCGGCACAACt > 1:858583/1‑149 (MQ=255) ttCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTCAATTTCTTCCGCCACGCGTTCAGGATCGGCTGCCGGCAGGTCAATCTTGTTCAGTACCGGCACAACtt > 3:343925/1‑149 (MQ=255) cGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTCAATTTCTTCCGCCACGCGTTCAGGATCGGCTGCCGGCAGGTCAATCTTGTTCAGTACCGGCACAACTTCg < 2:475933/149‑1 (MQ=255) cGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTCAATTTCTTCCGCCACGCGTTCAGGATCGGCTGCCGGCAGGTCAATCTTGTTCAGTACCGGCACAACTTCg < 1:214831/149‑1 (MQ=255) gagaACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTCAATTTCTTCCGCCACGCGTTCAGGATCGGCTGCCGGCAGGTCAATCTTGTTCAGTACCGGCACAACTTCga < 1:654543/149‑1 (MQ=255) gagaACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTCAATTTCTTCCGCCACGCGTTCAGGATCGGCTGCCGGCAGGTCAATCTTGTTCAGTACCGGCACAACTTCga > 3:100843/1‑149 (MQ=255) | GTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTCAATTTCTTCCGCCACGCGTTCAGGATCGGCTGCCGGCAGGTCAATCTTGTTCAGTACCGGCACAACTTCGA > NC_000913/2706462‑2706754 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |