Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 368,461 T→A 6.2% intergenic (‑41/‑150) mhpR ← / → mhpA mhp operon transcriptional activator/3‑(3‑hydroxyphenyl)propionate hydroxylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913368,4610TA 6.2% 363.7 / 7.6 108intergenic (‑41/‑150)mhpR/mhpAmhp operon transcriptional activator/3‑(3‑hydroxyphenyl)propionate hydroxylase
Reads supporting (aligned to +/- strand):  ref base T (46/55);  new base A (5/2);  total (51/57)
Fisher's exact test for biased strand distribution p-value = 2.51e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01

Only 100 of 110 total aligned reads displayed.

CAGCCCGACGCTGGCACCGCCATCAAGTTTATTTAACATATTTAATAACATTAGACCGCGGGTTAAGCCGCGCACGGTTTTGTATTCCGTCTGCTCATTGTTCTGCATATTAATTGACATTTCTATAGTTAAAACAACGTGGTGCACCTGGTGCACATTCGGGCATGTTTTGATTGTAGCCGAAAACACCCTTCCTATACTGAGCGCACAATAAAAAATCATTTACATGTTTTTAACAAAATAAGTTGCGCTGTACTGTGCGCGCAACGACATTTTGTCCGAGTCGTGAGGTACTGA  >  NC_000913/368313‑368609
                                                                                                                                                    |                                                                                                                                                    
cagcCCGACGCTGGCACCGCCATCAAGTTTATTTAACATATTTAATAACATTAGACCGCGGGTTAAGCCGCGCACGGTTTTGTATTCCGTCTGCTCATTGTTCTGCATATTAATTGACATTTCTATAGTTAAAACAACGTGGTGCACCt                                                                                                                                                      <  1:400948/149‑1 (MQ=255)
   cccGACGCTGGCACCGCCATCAAGTTTATTTAACATTTTTAATAACATTAGACCGCGGGTTAAGCCGCGCACGGTTTTGTATTCCGTCTGCTCATTGTTCTGCATATTAATTGACATTTCTATAGTTAAAACAACGTGGTGCACCTGGt                                                                                                                                                   <  3:802681/149‑1 (MQ=255)
   cccGACGCTGGCACCGCCATCAAGTTTATTTAACATATTTAATAACATTAGACCGCGGGTTAAGCCGCGCACGGTTTTGTATTCCGTCTGCTCATTGTTCTGCATATTAATTGACATTTCTATAGTTAAAACAACGTGGTGCACCTGGt                                                                                                                                                   >  3:732804/1‑149 (MQ=255)
    ccGACGCTGGCACCGCCATCAAGTTTATTTAACATATTTAATAACATTAGACCGCGGGTTAAGCCGCGCACGGTTTTGTATTCCGTCTGCTCATTGTTCTGCACATTAATTGACATTTCTATAGTTAAAACAACGTGGTGCACCTGGTg                                                                                                                                                  <  2:827964/149‑1 (MQ=255)
     cGACGCTGGCACCGCCATCAAGTTTATTTAACATATTTAATAACATTAGACCGCGGGTTAAGCCGCGCACGGTTTTGTATTCCGTCTGCTCATTGTTCTGCATATTAATTGACATTTCTATAGTTAAAACAACGTGGTGCACCTGGTGc                                                                                                                                                 <  1:542180/149‑1 (MQ=255)
     cGACGCTGGCACCGCCATCAAGTTTATTTAACATATTTAATAACATTAGACCGCGGGTTAAGCCGCGCACGGTTTTGTATTCCGTCTGCTCATTGTTCTGCATATTAATTGACATTTCTATAGTTAAAACAACGTGGTGCACCTGGTGc                                                                                                                                                 <  3:273081/149‑1 (MQ=255)
             gCACCGCCATCAAGTTTATTTAACATATTTAATAACATTAGACCGCGGGTTAAGCCGCGCACGGTTTTGTATTCCGTCTGCTCATTGTTCTGCATATTAATTGACATTTCTATAGTTAAAACAACGTGGTGCACCTGGTGCACATTCgg                                                                                                                                         <  2:935935/149‑1 (MQ=255)
             gCACCGCCATCAAGTTTATTTAACATATATAATAACATTAGACCGCGGGTTAAGCCGCGCACGGTTTTGTATTCCGTCTGCTCATTGTTCTGCATATTAATTGACATTTCTATAGTTAAAACAACGTGGTGCACCTGGTCCACATTCgg                                                                                                                                         <  4:795613/149‑1 (MQ=255)
               aCCGCCATCAAGTTTATTTAACATATTTAATAACATTAGACCGCGGGTTAAGCCGCGCACGGTTTTGTATTCCGTCTGCTCATTGTTCTGCATATTAATTGACATTTCTATAGTTAAAACAACGTGGTGCACCTGGTGCACATTCGGGc                                                                                                                                       >  3:868900/1‑149 (MQ=255)
               aCCGCCATCAAGTTTATTTAACATATTTAATAACATTAGACCGCGGGTTAAGCCGCGCACGGTTTTGTATTCCGTCTGCTCATTGTTCTGCATATTAATTGACATTTCTATAGTTAAAACAACGTGGTGCACCTGGTGCACATTCGGGc                                                                                                                                       >  3:45684/1‑149 (MQ=255)
                 cGCCATCAAGTTTATTTAACATATTTAATAACATTAGACCGCGGGTTAAGCCGCGCACGGTTTTGTATTCCGTCTGCTCATTGTTCTGCATATTAATTGACATTTCTATAGTTAAAACAACGTGGTGCACCTGGTGCACATTCGGGCAt                                                                                                                                     >  4:831693/1‑149 (MQ=255)
                  gCCATCAAGTTTATTTAACATATTTAATAACATTAGACCGCGGGTTAAGCCGCGCACGGTTTTGTATTCCGTCTGCTCATTGTTCTGCATATTAATTGACATTTCTATAGTTAAAACAACGTGGTGCACCTGGTGCACATTCGGGCATg                                                                                                                                    <  2:621443/149‑1 (MQ=255)
                      tCAAGTTTATTTAACATATTTAATAACATTAGACCGCGGGTTAAGCCGCGCACGGTTTTGTATTCCGTCTGCTCATTGTTCTGCATATTAATTGACATTTCTATAGTTAAAACAACGTGGTGCACCTGGTGCACATTCGGGCATGtttt                                                                                                                                >  1:659060/1‑149 (MQ=255)
                       cAAGTTTATTTAACATATTTAATAACATTAGACCGCGGGTTAAGCCGCGCACGGTTTTGTATTCCGTCTGCTCATTGTTCTGCATATTAATTGACATTTCTATAGTTAAAACAACGTGGTGCACCTGGTGCACATTCGGGCATGTTTTg                                                                                                                               <  1:7130/149‑1 (MQ=255)
                        aaGTTTATTTAACATATTTAATAACATTAGTCCGCGGGTTAAGCCGCGCACGGTTTTGTATTCCGTCTGCTCATTGTTCTGCATATTAATTGACATTTCTATAGTTAAAACAACGTGGTGCACCTGGTGCACATTCGGGCATGTTTTGa                                                                                                                              <  1:140191/149‑1 (MQ=255)
                        aaGTTTATTTAACATATTTAATAACATTAGACCGCGGGTTAAGCCGCGCACGGTTTTGTATTCCGTCTGCTCATTGTTCTGCATATTAATTGACATTTCTATAGTTAAAACAACGTGGTGCACCTGGTGCACATTCGGGCATGTTTTGa                                                                                                                              <  4:620207/149‑1 (MQ=255)
                        aaGTTTATTTAACATATTTAATAACATTAGACCGCGGGTTAAGCCGCGCACGGTTTTGTATTCCGTCTGCTCATTGTTCTGCATATTAATTGACATTTCTATAGTTAAAACAACGTGGTGCACCTGGTGCACATTCGGGCATGTTTTGa                                                                                                                              <  2:615830/149‑1 (MQ=255)
                        aaGTTTATTTAACATATTTAATAACATTAGACCGCGGGTTAAGCCGCGCACGGTTTTGTATTCCGTCTGCTCATTGTTCTGCATATTAATTGACATTTCTATAGTTAAAACAACGTGGTGCACCTGGTGCACATTCGGGCATGTTTTGa                                                                                                                              <  2:157736/149‑1 (MQ=255)
                        aaGTTTATTTAACATATTTAATAACATTAGACCGCGGGTTAAGCCGCGCACGGTTTTGTATTCCGTCTGCTCATTGTTCTGCATATTAATTGACATTTCTATAGTTAAAACAACGTGGTGCACCTGGTGCACATTCAGGCATGTTTTGa                                                                                                                              <  1:882680/149‑1 (MQ=255)
                         aGTTTATTTAACATATTTAATAACATTAGACCGCGGGTTAAGCCGCGCACGGTTTTGTATTCCGTCTGCTCATTGTTCTGCATATTAATTGACATTTCTATAGTTAAAACAACGTGGTGCACCTGGTGCACATTCGGGCATGTTTTGAt                                                                                                                             <  3:238476/149‑1 (MQ=255)
                         aGTTTATTTAACATATTTAATAACATTAGACCGCGGGTTAAGCCGCGCACGGTTTTGTATTCCGTCTGCTCATTGTTCTGCATATTAATTGACATTTCTATAGTTAAAACAACGTGGTGCACCTGGTGCACATTCGGGCATGTTTTGAt                                                                                                                             <  4:728395/149‑1 (MQ=255)
                          gTTTATTTAACATATTTAATAACATTAGACCGCGGGTTAAGCCGCGCACGGTTTTGTATTCCGTCTGCTCATTGTTCTGCATATTAATTGACATTTCTATAGTTAAAACAACGTGGTGCACCTGGTGCACATTCGGGCATGTTTTGAtt                                                                                                                            >  2:524748/1‑149 (MQ=255)
                          gTTTATTTAACATATTTAATAACATTAGACCGCGGGTTAAGCCGCGCACGGTTTTGTATTCCGTCTGCTCATTGTTCTGCATATTAATTGACATTTCTATAGTTAAAACAACGTGGTGCACCTGGTGCACATTCGGGCATGTTTTGAtt                                                                                                                            <  2:886276/149‑1 (MQ=255)
                          gTTTATTTAACATATTTAATAACATTAGACCGCGGGTTAAGCCGCGCACGGTTTTGTATTCCGTCTGCTCATTGTTCTGCATATTAATTGACATTTCTATAGTTAAAACAACGTGGTGCACCTGGTGCACATTCGGGCATGTTTTGAtt                                                                                                                            <  3:225545/149‑1 (MQ=255)
                           tttatttaACATATTTAATAACATTAGACCGCGGGTTAAGCCGCGCACGGTTTTGTATTCCGTCTGCTCATTGTTCTGCATATTAATTGACATTTCTATAGTTAAAACAACGTGGTGCACCTGGTGCACATTCGGGCATGTTTTGATTg                                                                                                                           >  1:250513/1‑149 (MQ=255)
                           tttatttaACATATTTAATAACATTAGACCGCGGGTTAAGCCGCGCACGGTTTTGTATTCCGTCTGCTCATTGTTCTGCATATTAATTGACATTTCTATAGTTAAAACAACGTGGTGCACCTGGTGCACATTCGGGCATGTTTTGATTg                                                                                                                           >  3:616550/1‑149 (MQ=255)
                            ttatttaACATATTTAATAACATTAGACCGCGGGTTAAGCCGCGCACGGTTTTGTATTCCGTCTGCTCATTGTTCTGCATATTAATTGACATTTCTATAGTTAAAACAACGTGGTGCACCTGGTGCACATTCGGGCATGTTTTGATTGt                                                                                                                          <  1:825287/149‑1 (MQ=255)
                              atttaACATATTTAATAACATTAGACCGCGGGTTAAGCCGCGCACGGTTTTGTATTCCGTCTGCTCATTGTTCTGCATATTAATTGACATTTCTATAGTTAAAACAACGTGGTGCACCTGGTGCACATTCGGGCATGTTTTGATTGTAg                                                                                                                        <  4:616550/149‑1 (MQ=255)
                               tttaACATATTTAATAACATTAGACCGCGGGTTAAGCCGCGCACGGTTTTGTATTCCGTCTGCTCATTGTTCTGCATATTAATTGACATTTCTATAGTTAAAACAACGTGGTGCACCTGGTGCACATTCGGGCATGTTTTGATTGTAGc                                                                                                                       <  3:738524/149‑1 (MQ=255)
                                   aCATATTTAATAACATTGGACCGCGGGTTAAGCCGCGCACGGTTTTGTATTCCGTCTGCTCATTGTTCTGCATATTAATTGACATTTCTATAGTTAAAACAACGTGGTGCACCTGGTGCACATTCGGGCATGTTTTGATTGTAGCCGaa                                                                                                                   >  4:208944/1‑149 (MQ=255)
                                     gtatTTAATAACATTAGACCGCGGGTTAAGCCGCGCACGGTTTTGTATTCCGTCTGCTCATTGTTCTGCATATTTATTGACATTTCTATAGTTAAAACAATGTGGTGCACCAGGTGCACATTCGTGCATGTATCGATTGTAGCCGaaaa                                                                                                                 >  2:916449/2‑149 (MQ=255)
                                     atatTTAATAACATTAGACCGCGGGTTAAGCCGCGCACGGTTTTGTATTCCGTCTGCTCATTGTTCTGCATATTAATTGACATTTCTATAGTTAAAACAACGTGGTGCACCTGGTGCACATTCGGGCATGttt                                                                                                                                 >  4:793982/1‑133 (MQ=255)
                                     atatTTAATAACATTAGACCGCGGGTTAAGCCGCGCACGGTTTTGTATTCCGTCTGCTCATTGTTCTGCATATTAATTGACATTTCTATAGTTAAAACAACGTGGTGCACCTGGTGCACATTCGGGCATGttt                                                                                                                                 <  3:793982/133‑1 (MQ=255)
                                     atatTTAATAACATTAGACCGCGGGTTAAGCCGCGCACGGTTTTGTATTCCGTCTGCTCATTGGTCTGCATATTAATTGACATTTCTATAGTTAAAACAACGTGGTGCACCTGGTGCACATTCGGGCATGTTTTGATTGTAGCCGaaaa                                                                                                                 >  4:632629/1‑149 (MQ=255)
                                      tatTTAATAACATTAGACCGCGGGTTAAGCCGCGCACGGTTTTGTATTCCGTCTGCTCATTGTTCTGCATATTAATTGACATTTCTATAGTTAAAACAACGTGGTGCACCTgg                                                                                                                                                    >  1:807694/1‑113 (MQ=255)
                                      tatTTAATAACATTAGACCGCGGGTTAAGCCGCGCACGGTTTTGTATTCCGTCTGCTCATTGTTCTGCATATTAATTGACATTTCTATAGTTAAAACAACGTGGTGCACCTgg                                                                                                                                                    <  2:807694/113‑1 (MQ=255)
                                        tttAATAACATTAGACCGCGGGTTAAGCCGCGCACGGTTTTGTATTCCGTCTGCTCATTGTTCTGCATATTAATTGACATTTCTATAGTTAAAACAACGTGGTGCACCTGGTGCACATTCGGGCATGTTTTGATTGTAGCCGAAAacac                                                                                                              >  4:407653/1‑149 (MQ=255)
                                        tttAATAACATTAGACCGCGGGTTAAGCCGCGCACGGTTTTGTATTCCGTCTGCTCATTGTTCTGCATATTAATTGACATTTCTATAGTTAAAACAACGTGGTGCACCTGGTGCACATTCGGGCATGTTTTGATTGTAGCCGAAAacac                                                                                                              <  4:45684/149‑1 (MQ=255)
                                         ttAATAACATTAGACCGCGGGTTAAGCCGCGCACGGTTTTGTATTCCGTCTGCTCATTGTTCTGCATATTAATTGACATTTCTATAGTTAAAACAACGTGGTGCACCTGGTGCACATTCGGGCATGTTTTGATTGTAGCCGAAAACAcc                                                                                                             >  4:164308/1‑149 (MQ=255)
                                           aataaCATTAGACCGCGGGTTAAGCCGCGCACGGTTTTGTATTCCGTCTGCTCATTGTTCTGCATATTAATTGACATTTCTATAGTTAAAACAACGTGGTGCACCTGGTGCACATTCGGGCATGTTTTGATTGTAGCCGAAAACACCCt                                                                                                           >  1:623421/1‑149 (MQ=255)
                                            ataaCATTAGACCGCGGGTTAAGCCGCGCACGGTTTTGTATTCCGTCTGCTCATTGTTCTGCATATTAATTGACATTTCTATAGTTAAAACAACGTGGTGCACCTGGTGCACATTCgg                                                                                                                                         >  3:143444/1‑118 (MQ=255)
                                            ataaCATTAGACCGCGGGTTAAGCCGCGCACGGTTTTGTATTCCGTCTGCTCATTGTTCTGCATATTAATTGACATTTCTATAGTTAAAACAACGTGGTGCACCTGGTGCACATTCgg                                                                                                                                         <  4:143444/118‑1 (MQ=255)
                                             taaCATTAGACCGCGGGTTAAGCCGCGCACGGTTTTGTATTCCGTCTGCTCATTGTTCTGCATATTAATTGACATTTCTATAGTTAAAACAACGTGGTGCACCTGGTGCACATTCGGGCATGTTTTGATTGTAGCCGAAAACACCCTTc                                                                                                         <  3:632629/149‑1 (MQ=255)
                                             taaCATTAGACCGCGGGTTAAGCCGCGCACGGTTTTGTATTCCGTCTGCTCATTGTTCTGCATATTAATTGACATTTCTATAGTTAAAACAACGTGGTGCACCTGGTGCACATTCGGGCATGTTTTGATTGTAGCCGAAAACACCCTTc                                                                                                         <  2:165669/149‑1 (MQ=255)
                                             taaCATTAGACCGCGGGTTAAGCCGCGCACGGTTTTGTATTCCGTCTGCTCATTGTTCTGCATATTAATTGACATTTCTATAGTTAAAACAACGTGGTGCACCTGGTGCACATTCGGGCATGTTTTGATTGTAGCCGAAAACACCCTTc                                                                                                         <  1:104385/149‑1 (MQ=255)
                                               aCATTAGACCGCGGGTTAAGCCGCGCACGGTTTTGTATTCCGTCTGCTCATTGTTCTGCATATTAATTGACATTTCTATAGTTAAAACAACGTGGTGCACCTGGTGCACATTCGGGCATGTTTTGATTGTAGCCGAAAACACCCTTCCt                                                                                                       <  3:527496/149‑1 (MQ=255)
                                                cATTAGACCGCGGGTTAAGCCGCGCACGGTTTTGTATTCCGTCTGCTCATTGTTCTGCATATTAATTGACATTTCTATAGTTAAAACAACGTGGTGCACCTGGTGCACATTCGGGCATGTTTTGATTGTAGCCGAAAACACCCTTCCta                                                                                                      <  1:936398/149‑1 (MQ=255)
                                                 aTTAGACCGCGGGTTAAGCCGCGCACGGTTTTGTATTCCGTCTGCTCATTGTTCTGCATATTAATTGACATTTCTATAGTTAAAACAACGTGGTGCACCTGGTGCACATTCGGGCATGTTTTGATTGTAGCCGAAAACACCCTTCCtat                                                                                                     >  3:153497/1‑149 (MQ=255)
                                                  ttAGACCGCGGGTTAAGCCGCGCACGGTTTTGTATTCCGTCTGCTCATTGTTCTGCATATTAATTGACATTTCTATAGTTAAAACAACGTGGTGCACCTGGTGCACATTCGGGCATGTTTTGATTGTAGCCGAAAACACCCTTCCtata                                                                                                    <  1:113088/149‑1 (MQ=255)
                                                  ttAGACCGCGGGTTAAGCCGCGCACGGTTTTGTATTCCGTCTGCTCATTGTTCTGCATATTAATTGACATTTCTATAGTTAAAACAACGTGGTGCACCTGGTGCACATTCGGGCATGTTTTGATTGTAGCCGAAAACACCCTTCCtata                                                                                                    <  3:254698/149‑1 (MQ=255)
                                                    aGACCGCGGGTTAAGCCGCGCACGGTTTTGTATTCCGTCTGCTCATTGTTCTGCATATTAATTGACATTTCTATAGTTAAAACAACGTGGTGCACCTGGTGCACATTCGGGCATGTTTTGATTGTAGCCGAAAACACCCTTCCTATACt                                                                                                  <  1:139848/149‑1 (MQ=255)
                                                     gACCGCGGGTTAAGCCGCGCACGGTTTTGTATTCCGTCTGCTCATTGTTCTGCATATTAATTGACATTTCTATAGTTAAAACAACGTGGTGCACCTGGTGCACATTCGGGCATGTTTTGATTGTAGCCGAAAACACCCTTCCTATACTg                                                                                                 >  2:411429/1‑149 (MQ=255)
                                                                 aGCCGCGCACGGTTTTGTATTCCGTCTGCTCATTGTTCTGCATATTAATTGACATTTCTATAGTTAAAACAACGTGGTGCACCTGGTGCACATTCGGGCATGTTTTGATTGTAGCCGAAAACACCCtt                                                                                                          <  1:532449/128‑1 (MQ=255)
                                                                 aGCCGCGCACGGTTTTGTATTCCGTCTGCTCATTGTTCTGCATATTAATTGACATTTCTATAGTTAAAACAACGTGGTGCACCTGGTGCACATTCGGGCATGTTTTGATTGTAGCCGAAAACACCCtt                                                                                                          >  2:532449/1‑128 (MQ=255)
                                                                  gCCGCGCACGGTTTTGTATTCCGTCTGCTCATTGTTCTGCATATTAATTGACATTTCTATAGTTAAAACAACGTGGTGCACCTGGTGCACATTCGGGCATGTTTTGATTGTAGcc                                                                                                                      >  2:839340/1‑115 (MQ=255)
                                                                  gCCGCGCACGGTTTTGTATTCCGTCTGCTCATTGTTCTGCATATTAATTGACATTTCTATAGTTAAAACAACGTGGTGCACCTGGTGCACATTCGGGCATGTTTTGATTGTAGcc                                                                                                                      <  1:839340/115‑1 (MQ=255)
                                                                  gCCGCGCACGGTTTTGTATTCCGTCTGCTCATTGTTCTGCATATTAATTGACATTTCTATAGTTAAAACAACGTGGTGCACCTGGTGCACATTCGGGCATGTTTTGATTGTAGCCGAAAACACCCTTCCTATACTGAGCGCACAATaaa                                                                                    >  1:188994/1‑149 (MQ=255)
                                                                  gCCGCGCACGGTTTTGTATTCCGTCTGCTCATTGTTCTGCATATTAATTGACATTTCTATAGTTAAAACAACGTGGTGCACCTGGTGCACATTCGGGCATGTTTTGATTGTAGCCGAAAACACCCTTCCTATACTGAGCGCACAATaaa                                                                                    >  2:652689/1‑149 (MQ=255)
                                                                  gCCGCGCACGGTTTAGTATTCCGTCTGCTCATTGTTCTGCATATTAATTGACATTTCTATAGTTAAAACAACGTGGTGCACCTGGTGCACATTCGGGCATGTTTTGATTGTAGCCGAAAACACCCTTCCTATACTGAGCGCACAATaaa                                                                                    >  1:701781/1‑149 (MQ=255)
                                                                     gcgcACGGTTTTGTATTCCGTCTGCTCATTGTTCTGCATATTAATTGACATTTCTATAGTTAAAACAACGTGGTGCACCTGGTGCACATTCGGGCATGTTTTGATTGTAGCCGAAAACACCCTTCCTATACTGAGCGCACAATaaaaaa                                                                                 >  3:119264/1‑149 (MQ=255)
                                                                      cgcACGGTTTTGTATTCCGTCTGCTCATTGTTCTGCATATTAATTGACATTTCTATAGTTAAAACAACGTGGTGCACCTGGTGCACATTCGGGCATGTTTTGATTGTAGCCGAAAACACCCTTCCTATACTGAGCGCACAATAAAAAAt                                                                                <  4:868900/149‑1 (MQ=255)
                                                                       gcACGGTTTTGTATTCCGTCTGCTCATTGTTCTGCATATTAATTGACATTTCTATAGTTAAAACAACGTGGTGCACCTGGTGCACATTCGGGCATGTTTTGATTGTAGCCGAAAACACCCTTCCTATACTGAGCGCACAATAAAAAATc                                                                               <  1:808771/149‑1 (MQ=255)
                                                                         aCGGTTTTGTATTCCGTCTGCTCATTGTTCTGCATATTAATTGACATTTCTATAGTTAAAACAACGTGGTGCACCTGGTGCACATTCGGGCATGTTTTGATTGTAGCCGAAAACACCCTTCCTATACTGAGCGCACAATAAAAAATCAt                                                                             >  2:734120/1‑149 (MQ=255)
                                                                          cagTTTTGTATTCCGTCTACTAATTGTTCTGCATATTAATTGACATTTCTATATTTAAAACAACGTAGTGCACCTAATGCACATTCGGGCATGTTTTGAATGTAGCCGAAAACACCCATCTTATACTGAGCGCACAAGAAAAAATCAtt                                                                            <  2:376817/147‑1 (MQ=255)
                                                                          cGGTTTTGTATTCCGTCTGCTCATTGTTCTGCATATTAATTGACATTTCTATAGTTAAAACAACGTGGTGCACCTGGTGCACATTCGGGCATGTTTTGATTGTAGCCGAAAACACCCTTCCTATACTGAGCGCGCAATAAAAAATCAtt                                                                            <  4:885723/149‑1 (MQ=255)
                                                                          cGGTTTTGTATTCCGTCTGCTCATTGTTCTGCATATTAATTGACATTTCTATAGTTAAAACAACGTGGTGCACCTGGTGCACATTCGGGCATGTTTTGATTGTAGCCGAAAACACCCTTCCTATACTGAGCGCACAATAAAAAATCAtt                                                                            <  4:638699/149‑1 (MQ=255)
                                                                             ttttGTATTCCGTCTGCTCATTGTTCTGCATATTAATTGACATTTCTATAGTTAAAACAACGTGGTGCACCTGGTGCACATTCGGGCATGTTTTGATTGTAGCCGAAAACACCCTTCCTATACTGAGCGCACAATAAAAAATCATTTAc                                                                         >  3:308412/1‑149 (MQ=255)
                                                                                  tATTCCGTCTGCTCATTGTTCTGCATATTAATTGACATTTCTATAGTTAAAACAACGTGGTGCACCTGGTGCACATTCGGGCATGTTTTGATTGTAGCCGAAAACACCCTTCCTATACTGAGCGCACAATAAAAAATCATTTACATGtt                                                                    <  4:899158/149‑1 (MQ=255)
                                                                                   aTTCCGTCTGCTCATTGTTCTGCATATTAATTGGCATTTCTATAGTTAAAACAACGTGGTGCACCTGGTCCACATTCGGGCATGTTTTGATTGTAGCCGAAAACACCCTTCCTATACTGAGCGCACAATAAAAAATCATTTACATGttt                                                                   <  1:916449/149‑1 (MQ=255)
                                                                                    ttCCGTCTGCTCATTGTTCTGCATATTAATTGACATTTCTATAGTTAAAACAACGTGGTGCACCTGGTGCACATTCGGGCATGTTTTGATTGTAGCCGAAAACACCCTTCCTATACTGAGCGCACAATAAAAAATCATTTACAt                                                                       >  4:707648/1‑144 (MQ=255)
                                                                                    ttCCGTCTGCTCATTGTTCTGCATATTAATTGACATTTCTATAGTTAAAACAACGTGGTGCACCTGGTGCACATTCGGGCATGTTTTGATTGTAGCCGAAAACACCCTTCCTATACTGAGCGCACAATAAAAAATCATTTACATGtttt                                                                  >  2:293808/1‑149 (MQ=255)
                                                                                        gTCTGCTCATTGTTCTGCATATTAATTGACATTTCTATAGTTAAAACAACGTGGTGCACCTGGTGCACATTCGGGCATGTTTTGATTGTAGCCGAAAACACCCTTCCTATACTGAGCGCACAATAAAAAATCATTTACATGTTTTTAAc                                                              >  3:238789/1‑149 (MQ=255)
                                                                                             cTCATTGTTCTGCATATTAATTGACATTTCTATAGTTAAAACAACGTGGTGCACCTGGTGCACATTCg                                                                                                                                          >  2:356759/1‑68 (MQ=255)
                                                                                             cTCATTGTTCTGCATATTAATTGACATTTCTATAGTTAAAACAACGTGGTGCACCTGGTGCACATTCGGGCATGTTTTGATTGTAGCCGAAAACACCCTTCCTATACTGAGCGTACAATAAAAAATCATTTACATGTTTTTAACAAAAt                                                         >  4:345245/1‑149 (MQ=255)
                                                                                              tCATTGTTCTGCATATTAATTGACATTCCTATAGTTAAAACAACGTGGTGCACCTGGTGCAGATTCGGGCATGTTTTGATTGTAGCCGAAAACACCCTTCCTATACTGAGCGCACAATAAAAAATCATTTACATGTTTTTAACAAAATa                                                        >  4:881853/1‑149 (MQ=255)
                                                                                                aTTGTTCTGCATATTAATTGACATTTCTATAGTTAAAACAACGTGGTGCACCTGGTGCACATTCGGGCATGTTTTGATTGTAGCCGAAAACACCCTTCCTATACTGAGCGCACAATAAAAAATCATTTa                                                                          >  3:321956/1‑129 (MQ=255)
                                                                                                aTTGTTCTGCATATTAATTGACATTTCTATAGTTAAAACAACGTGGTGCACCTGGTGCACATTCGGGCATGTTTTGATTGTAGCCGAAAACACCCTTCCTATACTGAGCGCACAATAAAAAATCATTTACATGTTTTTAACAAAATAAg                                                      >  2:295073/1‑149 (MQ=255)
                                                                                                           tatTAATTGACATTTCTATAGTTAAAACAACGTGGTGCACCTGGTGCACATTCGGGCATGTTTTGATTGTAGCCGAAAACACCCTTCCTATACTGAGCGCACAATAAAAAATCATTTACATGTTTTTAACAAAATAAGTTGCGCTGTAc                                           >  2:785586/1‑149 (MQ=255)
                                                                                                            atTAATTGACATTTCTATAGTTAAAACAACGTGGTGCACCTGGTGCACATTCGGGCATGTTTTGATTGTAGCCGAAAACACCCTTCCTATACTGAGCGCACAATAAAAAATCATTTACATGTTTTTAACAAAATAAGTTGCGCTGTACt                                          >  3:122479/1‑149 (MQ=255)
                                                                                                                aTTGACATTTCTATAGTTAAAACAACGTGGTGCACCTGGTGCACATTCGGGCATGTTTTGATTGTAGCCGAAAACACCCTTCCTATACTGAGCGCACAATAAAAAATCATTTACATGTTTTTAACAAAATAAGTTGCGCTGTACTGTgc                                      <  4:122479/149‑1 (MQ=255)
                                                                                                                      aTTTCTATAGTTAAAACAACGTGGTGCACCTGGTGCACATTCGGGCATGTTTTGATTGTAGCCGAAAACACCCTTCCTATACTGAGCGCACAATAAAAAATCATTTACATGTTTTTAACAAAATAAGTTGCGCTGTACTGTGCGCGCaa                                >  2:434402/1‑149 (MQ=255)
                                                                                                                      aTTTCTATAGTTAAAACAACGTGGTGCACCTGGTGCACATTCGGGCATGTTTTGATTGTAGCCGAAAACACCCTTCCTATACTGAGCGCACAATAAAAAATCATTTACATGTTTTTAACAAAATAAGTTGCGCTGTACTGTGCGCGCaa                                >  2:456727/1‑149 (MQ=255)
                                                                                                                           tataGTTAAAACAACGTGGTGCACCTGGTGCACATTCGGGCATGTTTTGATTGTAGCCGAAAACACCCTTCCTATACTGAGCGCACAATAAAAAATCATTTACATGTTTTTAACaaaa                                                          <  3:605877/118‑1 (MQ=255)
                                                                                                                           tataGTTAAAACAACGTGGTGCACCTGGTGCACATTCGGGCATGTTTTGATTGTAGCCGAAAACACCCTTCCTATACTGAGCGCACAATAAAAAATCATTTACATGTTTTTAACaaaa                                                          >  4:605877/1‑118 (MQ=255)
                                                                                                                             taGTTAAAACAACGTGGTGCACCTGGTGCACATTCGGGCATGTTTTGATTGTAGCCGAAAACACCCTTCCTATACTGAGCGCACAATAAAAAATCATTTACATGTTTTTAACAAAATAAGTTGCGCTGTACTGTGCGCGCAACGACAtt                         <  3:831693/149‑1 (MQ=255)
                                                                                                                               gTTAAAACAACGTGGTGCACCTGGTGCACATTCGGGCATGTTTTGATTGTAGCCGAAAACACCCTTCCTATACTGAGCGCACAATAAAAAATCATTTACATGTTTTTAACAAAATAAGTTGCGCTGTACTGTGCGCGCAACGACAtttt                       <  3:407653/149‑1 (MQ=255)
                                                                                                                                ttAAAACAACGTGGTGCACCTGGTGCACATTCGGGCATGTTTTGATTGTAGCCGAAAACACCCTTCCTATACTGAGCGCACAATAAAAAATCATTTACATGTTTTTAACAAAATAAGTTGCGCTGTACTGTGCGCGCAACGACATTTTg                      >  2:498923/1‑149 (MQ=255)
                                                                                                                                ttAAAACAACGTGGTGCACCTGGTGCACATTCGGGCATGTTTTGATTGTAGCCGAAAACACCCTTCCTATACTGAGCGCACAATAAAAAATCATTTACATGTGTTTAACAAAATAAGTTGCGCTGTACTGTGCGCGCAACGACATTTTg                      >  2:227952/1‑149 (MQ=255)
                                                                                                                                  aaaaCAACGTGGTGCACCTGGTGCACATTCGGGCATGTTTTGATTGTAGCCGAAAACACCCTTCCTATACTGAGCGCACAATAAAAAATCATTTACATGTTTTTAACAAAATAAGTTGCGCTGTACTGTGCGCGCAACGACATTTTGTc                    <  4:374556/149‑1 (MQ=255)
                                                                                                                                    aacaacGTGGTGCACCTGGTGCACATTCGGGCATGTTTTGATTGTAGCCGAAAACACCCTTCCTATACTGAGCGCACAATAAAAAATCATTTACATGTTTTTAACAAAATAAGTTGCGCTGTACTGTGCGCGCAACGACATTTTGTCCg                  >  4:582141/1‑149 (MQ=255)
                                                                                                                                    aacaacGTGGTGCACCTGGTGCACATTCGGGCATGTTTTGATTGTAGCCGAAAACACCCTTCCTATACTGAGCGCACAATAAAAAATCATTTACATGTTTTTAACAAAATAAGTTGCGCTGTACTGTGCGCGCAACGACATTTTGTCCg                  >  1:286120/1‑149 (MQ=255)
                                                                                                                                        cgaatgtgCACCAGGTGCACATTCGGGCATGTTTTGATTGTAGCCGAAAACACCCTTCCTATACTGAGCGCACAATAAAAAATCATTTACATGTTTTTAACAAAATAAGTTGCGCTGTACTGTGCGCGCAACGACATTTTGTc                    >  2:699289/6‑143 (MQ=255)
                                                                                                                                        cgaatgtgCACCAGGTGCACATTCGGGCATGTTTTGATTGTAGCCGAAAACACCCTTCCTATACTGAGCGCACAATAAAAAATCATTTACATGTTTTTAACAAAATAAGTTGCGCTGTACTGTGCGCGCAACGACATTTTGTc                    <  1:699289/138‑1 (MQ=255)
                                                                                                                                         gaatgtgCACCAGGTGCACATTCGGGCATGTTTTGATTGTAGCCGAAAACACCCTTCCTATACTGAGCGCGCAATAAAAAATCATTTACATGTTTTTAACAAAATAAGTTGCGTTGTACTGTGCGCGCAACGACATTTTGTCCGa                 <  2:798912/141‑1 (MQ=255)
                                                                                                                                         gaatgtgCACCAGGTGCACATTCGGGCATGCTTTGATTGTATCCGAAAACACCCTTCCTATACTGAGCGCACAATAAAAAATCATTTACATGTTTTTAACAAAATAAGTTGCGCTGTACTGTGCGCGCAACGACATTTTGTCCGAGTCg             >  1:194852/5‑149 (MQ=255)
                                                                                                                                          aatgtgCACCAGGTGCACATTCGGGCATGTTTTGATTGTAGCCGAAAACACCCTTCCTATACTGAGCGCACAATAAAAAATCATTTACATGTTTTTAACAAAATAAGTTGCGCTGTACTGTGCGCGCAACGACATTTTGTCCGAGTCGt            >  4:8168/4‑149 (MQ=255)
                                                                                                                                            ggtgCACCTGGTGCACATTCGGGCATGTTTTGATTGTAGCCGAAAACACCCTTCCTATACTGAGCGCACAATAAAAAATCATTTACATGTTTTTAACAAAATAAGTTGCGCTGTACTGTGCGCGCAACGACATTTTGTCCGAGTCGTGa          <  1:259958/149‑1 (MQ=255)
                                                                                                                                            ggtgCACCTGGTGCACATTCGGGCATGTTTTGATTGTAGCCGAAAACACCCTTCCTATACTGAGCGCACAATAAAAAATCATTTACATGTTTTTAACAAAATAAGTTGCGCTGTACTGTGCGCGCAACGACATTTTGTCCGAGTCGTGa          <  1:214247/149‑1 (MQ=255)
                                                                                                                                                  ccTGGTGCACATTCGGGCATGTTTTGATTGTAGCCGAAAACACCCTTCCTATACTGAGCGCACAATAAAAAATCATTTACATGTTTTTAACAAAATAAGTTGCGCTGTACTGTGCGCGCAACGACATTTTGTCCGAGTCGTGAGGTACt    >  1:709500/1‑149 (MQ=255)
                                                                                                                                                    tGGTGCACATTCGGGCATGTTTTGATTGTAGCCGAAAACACCCTTCCTATACTGAGCGCACAATAAAAAATCATTTACATGTTTTTAACAAAATAAGTTGCGCTGTACTGTGCGCGCAACGACATTTTGTCCGAGTCGTGAGGTACTGa  >  1:678938/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CAGCCCGACGCTGGCACCGCCATCAAGTTTATTTAACATATTTAATAACATTAGACCGCGGGTTAAGCCGCGCACGGTTTTGTATTCCGTCTGCTCATTGTTCTGCATATTAATTGACATTTCTATAGTTAAAACAACGTGGTGCACCTGGTGCACATTCGGGCATGTTTTGATTGTAGCCGAAAACACCCTTCCTATACTGAGCGCACAATAAAAAATCATTTACATGTTTTTAACAAAATAAGTTGCGCTGTACTGTGCGCGCAACGACATTTTGTCCGAGTCGTGAGGTACTGA  >  NC_000913/368313‑368609

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: